AlfalfaGEDB | Msa1239730

M. sativa cultivar zhongmu-4 / Msa1239730

KEGG: K08850
GO: GO:0000226 GO:0000228 GO:0000278 GO:0000775 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0005875 GO:0005876 GO:0006325 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0007052 GO:0008150 GO:0008152 GO:0009987 GO:0010564 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016569 GO:0016570 GO:0016572 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022402 GO:0031974 GO:0031981 GO:0032133 GO:0032465 GO:0032991 GO:0035173 GO:0035174 GO:0035175 GO:0035404 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043987 GO:0043988 GO:0044022 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0048471 GO:0050789 GO:0050794 GO:0051233 GO:0051276 GO:0051302 GO:0051726 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0098687 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140096 GO:1901564 GO:1902850 GO:1903047
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1239730	XP_003627679.2	90.244	123	4	1	1	115	1	123	4.08e-65	220
Msa1239730	XP_003627679.2	98.175	274	5	0	145	418	5	278	0.0	561

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1239730	sp\|O64629\|AUR3_ARATH	79.630	270	55	0	148	417	14	283	1.18e-163	464
Msa1239730	sp\|O64629\|AUR3_ARATH	68.966	116	28	1	8	115	14	129	9.49e-48	167

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1239730	Msa1281010	0.868693	5.402093e-66	-8.615850e-47
Msa1239730	Msa1321690	0.810231	1.277298e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1239730	MtrunA17_Chr8g0351491	98.175	274	5	0	145	418	5	278	0.0	561
Msa1239730	MtrunA17_Chr8g0351491	90.244	123	4	1	1	115	1	123	3.75e-69	220
Msa1239730	MtrunA17_Chr8g0353621	87.037	270	35	0	148	417	2	271	1.29e-178	498
Msa1239730	MtrunA17_Chr8g0353621	81.897	116	13	1	8	115	2	117	6.02e-58	191
Msa1239730	MtrunA17_Chr3g0125381	88.372	215	22	1	205	416	1	215	1.57e-138	395
Msa1239730	MtrunA17_Chr3g0125381	84.746	59	9	0	57	115	1	59	3.42e-26	105
Msa1239730	MtrunA17_Chr3g0140631	65.314	271	94	0	146	416	27	297	1.00e-133	385
Msa1239730	MtrunA17_Chr3g0140631	58.333	120	42	1	4	115	25	144	8.88e-39	141
Msa1239730	MtrunA17_Chr2g0304111	34.100	261	164	3	156	410	23	281	1.44e-54	187
Msa1239730	MtrunA17_Chr2g0304111	26.733	101	66	1	16	108	23	123	4.36e-11	64.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1239730	AT2G45490.1	79.630	270	55	0	148	417	14	283	1.19e-164	464
Msa1239730	AT2G45490.1	68.966	116	28	1	8	115	14	129	9.64e-49	167
Msa1239730	AT2G45490.2	80.498	241	47	0	148	388	14	254	1.62e-147	419
Msa1239730	AT2G45490.2	68.966	116	28	1	8	115	14	129	4.30e-49	167
Msa1239730	AT4G32830.1	65.056	269	94	0	148	416	23	291	7.64e-134	386
Msa1239730	AT4G32830.1	58.621	116	40	1	8	115	23	138	1.58e-38	140
Msa1239730	AT2G25880.1	65.056	269	94	0	148	416	17	285	1.09e-133	385
Msa1239730	AT2G25880.1	57.500	120	43	1	4	115	13	132	1.17e-38	141
Msa1239730	AT2G25880.4	65.056	269	94	0	148	416	30	298	2.78e-133	385
Msa1239730	AT2G25880.4	57.500	120	43	1	4	115	26	145	1.59e-38	141

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Find 122 sgRNAs with CRISPR-Local

Find 257 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGACAATGTGTGGAACAT+TGG	0.485065	8_2:+21660385	Msa1239730:CDS
AAAGAGAATGCAAACCATAC+GGG	0.560670	8_2:+21660901	Msa1239730:CDS
AAATCTGTTGCTTGACCATG+AGG	0.635996	8_2:+21660022	Msa1239730:CDS
AAATGGATCAAAATCAGAAA+CGG	0.491602	8_2:+21657040	Msa1239730:exon
AAGAATCAAGAAAACACGCT+CGG	0.619506	8_2:-21657598	None:intergenic
AAGAATCAAGAAAACACGCT+CGG	0.619506	8_2:-21659086	None:intergenic
AATATGCCAATGCCTTTGTG+AGG	0.683355	8_2:-21659959	None:intergenic
AATGGATCAAAATCAGAAAC+GGG	0.478303	8_2:+21657041	Msa1239730:exon
ACATCTTGCGTCTCTATGGT+TGG	0.508211	8_2:+21657565	Msa1239730:CDS
ACATCTTGCGTCTCTATGGT+TGG	0.508211	8_2:+21659053	Msa1239730:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATGGATCAAAATCAGAAAC+GGG	+	chr8_2:21657041-21657060	Msa1239730:exon	30.0%
AAACTTTCAGCAGTGAAAAA+GGG	-	chr8_2:21657174-21657193	None:intergenic	30.0%
AAAACTTTCAGCAGTGAAAA+AGG	-	chr8_2:21657175-21657194	None:intergenic	30.0%
TTATAGAGACTATAGAGTGT+TGG	+	chr8_2:21657266-21657285	Msa1239730:intron	30.0%
TTATGTTGTAGTAAGGACTA+AGG	+	chr8_2:21657365-21657384	Msa1239730:intron	30.0%
AGTAGATCAGGTAAATTTAG+CGG	+	chr8_2:21657390-21657409	Msa1239730:intron	30.0%
GTTAGAATGACATAAAAAGC+AGG	-	chr8_2:21657854-21657873	None:intergenic	30.0%
TCCATAACTTAGATACAGTT+AGG	-	chr8_2:21657920-21657939	None:intergenic	30.0%
TAACTGTATCTAAGTTATGG+AGG	+	chr8_2:21657919-21657938	Msa1239730:intron	30.0%
TCCATAACTTAGATACAGTT+AGG	-	chr8_2:21657920-21657939	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	21656849	21661233	21656849	ID=Msa1239730;Name=Msa1239730
chr8_2	mRNA	21656849	21661233	21656849	ID=Msa1239730-mRNA-1;Parent=Msa1239730;Name=Msa1239730-mRNA-1;_AED=0.22;_eAED=0.22;_QI=129\|0.66\|0.7\|1\|0.88\|0.8\|10\|297\|418
chr8_2	exon	21656849	21656965	21656849	ID=Msa1239730-mRNA-1:exon:7753;Parent=Msa1239730-mRNA-1
chr8_2	exon	21657030	21657152	21657030	ID=Msa1239730-mRNA-1:exon:7754;Parent=Msa1239730-mRNA-1
chr8_2	exon	21657477	21657720	21657477	ID=Msa1239730-mRNA-1:exon:7755;Parent=Msa1239730-mRNA-1
chr8_2	exon	21658401	21658480	21658401	ID=Msa1239730-mRNA-1:exon:7756;Parent=Msa1239730-mRNA-1
chr8_2	exon	21658572	21658667	21658572	ID=Msa1239730-mRNA-1:exon:7757;Parent=Msa1239730-mRNA-1
chr8_2	exon	21658941	21659183	21658941	ID=Msa1239730-mRNA-1:exon:7758;Parent=Msa1239730-mRNA-1
chr8_2	exon	21659948	21660043	21659948	ID=Msa1239730-mRNA-1:exon:7759;Parent=Msa1239730-mRNA-1
chr8_2	exon	21660332	21660546	21660332	ID=Msa1239730-mRNA-1:exon:7760;Parent=Msa1239730-mRNA-1
chr8_2	exon	21660679	21660751	21660679	ID=Msa1239730-mRNA-1:exon:7761;Parent=Msa1239730-mRNA-1
chr8_2	exon	21660838	21661233	21660838	ID=Msa1239730-mRNA-1:exon:7762;Parent=Msa1239730-mRNA-1
chr8_2	five_prime_UTR	21656849	21656965	21656849	ID=Msa1239730-mRNA-1:five_prime_utr;Parent=Msa1239730-mRNA-1
chr8_2	five_prime_UTR	21657030	21657041	21657030	ID=Msa1239730-mRNA-1:five_prime_utr;Parent=Msa1239730-mRNA-1
chr8_2	CDS	21657042	21657152	21657042	ID=Msa1239730-mRNA-1:cds;Parent=Msa1239730-mRNA-1
chr8_2	CDS	21657477	21657720	21657477	ID=Msa1239730-mRNA-1:cds;Parent=Msa1239730-mRNA-1
chr8_2	CDS	21658401	21658480	21658401	ID=Msa1239730-mRNA-1:cds;Parent=Msa1239730-mRNA-1
chr8_2	CDS	21658572	21658667	21658572	ID=Msa1239730-mRNA-1:cds;Parent=Msa1239730-mRNA-1
chr8_2	CDS	21658941	21659183	21658941	ID=Msa1239730-mRNA-1:cds;Parent=Msa1239730-mRNA-1
chr8_2	CDS	21659948	21660043	21659948	ID=Msa1239730-mRNA-1:cds;Parent=Msa1239730-mRNA-1
chr8_2	CDS	21660332	21660546	21660332	ID=Msa1239730-mRNA-1:cds;Parent=Msa1239730-mRNA-1
chr8_2	CDS	21660679	21660751	21660679	ID=Msa1239730-mRNA-1:cds;Parent=Msa1239730-mRNA-1
chr8_2	CDS	21660838	21660936	21660838	ID=Msa1239730-mRNA-1:cds;Parent=Msa1239730-mRNA-1
chr8_2	three_prime_UTR	21660937	21661233	21660937	ID=Msa1239730-mRNA-1:three_prime_utr;Parent=Msa1239730-mRNA-1

Gene Sequence

>Msa1239730

ATGGATCAAAATCAGAAACGGGAGGAGGAATGGTCGATCAACGACTTCGAGATCGGAAAGCCACTCGGGAGAGGCAAATTCGGCAGAGTCTATGTTGCCAGAGAAGTCAAGGTCATCTTTAAGGAACAACTAGAAAAGTATAACATTCTTCACCAACTGAGGAGGGAAATGGAGATACAGATCAGTCTTAAACATCCAAACATCTTGCGTCTCTATGGTTGGTTTCATGATGCCGAGCGTGTTTTCTTGATTCTTGAATATGCTCATAACGGTGAACTATACAAGGAGCTTCGCAAAAAAGGTCATTTCTCTGAGAAACAAGCTGCAACGGTAATCCTTTATTTGCTTGTTGAATCATTTCCCTCTCTATCTCTTTCTCGTGCGATTCTCCTCCGCCAGCCACGTTCGGAGCGCCTCCGTCACATTTACAAACAGAAACGGGAGGAGGAATGGTCGATCAACGACTTCGAGATCGGAAAGCCACTCGGGAGAGGCAAATTCGGCAGAGTCTATGTTGCCAGAGAAGTCAAGAGTAAATATGTGATTGCATTGAAGGTCATCTTTAAGGAACAACTAGAAAAGTATAACATTCTTCACCAACTGAGGAGGGAAATGGAGATACAGATCAGTCTTAAACATCCAAACATCTTGCGTCTCTATGGTTGGTTTCATGATGCCGAGCGTGTTTTCTTGATTCTTGAATATGCTCATAACGGTGAACTATACAAGGAGCTTCGCAAAAAAGGTCATTTCTCTGAGAAACAAGCTGCAACGTACATTTTAAGCCTCACAAAGGCATTGGCATATTGTCACGAGAAGCATGTTATTCACAGAGATATCAAGCCTGAAAATCTGTTGCTTGACCATGAGGGTCGTCTTAAGATTGCAGACTTTGGTTGGTCTGTACAATCTGTAAAAAAAAGAAAGACAATGTGTGGAACATTGGATTATTTAGCTCCTGAAATGGTAGAAAACAAAGGTCATGATTATGCAGTTGATAACTGGACTCTGGGTATTCTTTGTTATGAGTTCCTCTATGGTGCTCCTCCATTTGAGGCTGAGAGTCAAGAAGATACCTTCAAAAGGATAAGAGATGTTGATCTGAACTTCCCTCCTACCCCTCATGTTTCTAAGAATGCCAAAAATCTGATAAGTCAGCTACTGGTAAAGGATTCTTCAAAAAGGCTCTCACTTCAGAAGATAATGGAGCATCCTTGGATTAAAGAGAATGCAAACCATACGGGCGTCTGCGAATAG

Protein sequence

>Msa1239730

MDQNQKREEEWSINDFEIGKPLGRGKFGRVYVAREVKVIFKEQLEKYNILHQLRREMEIQISLKHPNILRLYGWFHDAERVFLILEYAHNGELYKELRKKGHFSEKQAATVILYLLVESFPSLSLSRAILLRQPRSERLRHIYKQKREEEWSINDFEIGKPLGRGKFGRVYVAREVKSKYVIALKVIFKEQLEKYNILHQLRREMEIQISLKHPNILRLYGWFHDAERVFLILEYAHNGELYKELRKKGHFSEKQAATYILSLTKALAYCHEKHVIHRDIKPENLLLDHEGRLKIADFGWSVQSVKKRKTMCGTLDYLAPEMVENKGHDYAVDNWTLGILCYEFLYGAPPFEAESQEDTFKRIRDVDLNFPPTPHVSKNAKNLISQLLVKDSSKRLSLQKIMEHPWIKENANHTGVCE*