AlfalfaGEDB | Msa1243620

M. sativa cultivar zhongmu-4 / Msa1243620

KEGG: -
GO: GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF01453.27

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1243620	GAU16886.1	54.805	385	95	6	136	498	1	328	5.61e-117	372
Msa1243620	GAU16886.1	70.079	381	73	5	590	962	2	349	1.27e-173	519

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1243620	sp\|Q39086\|SD17_ARATH	50.769	130	60	1	373	498	691	820	3.82e-32	138
Msa1243620	sp\|Q39086\|SD17_ARATH	35.075	134	69	5	819	941	694	820	2.43e-13	78.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1243620	Msa1324340	0.845294	4.441499e-59	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1243620	MtrunA17_Chr8g0357711	68.551	283	64	6	590	861	227	495	2.01e-108	354
Msa1243620	MtrunA17_Chr8g0357711	54.305	302	104	10	103	377	187	481	6.45e-77	268
Msa1243620	MtrunA17_Chr8g0357711	88.889	126	14	0	373	498	650	775	6.67e-65	234
Msa1243620	MtrunA17_Chr8g0357711	87.619	105	13	0	858	962	692	796	1.55e-50	192
Msa1243620	MtrunA17_Chr8g0357711	57.971	138	41	4	1	124	1	135	1.19e-35	145
Msa1243620	MtrunA17_Chr8g0357711	58.333	108	41	2	533	637	40	146	5.40e-28	121
Msa1243620	MtrunA17_Chr8g0357761	67.138	283	68	6	590	861	230	498	1.14e-100	333
Msa1243620	MtrunA17_Chr8g0357761	53.974	302	105	10	103	377	190	484	5.82e-74	260
Msa1243620	MtrunA17_Chr8g0357761	91.270	126	11	0	373	498	653	778	1.08e-65	236
Msa1243620	MtrunA17_Chr8g0357761	69.178	146	41	2	819	962	656	799	2.53e-52	197

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1243620	AT1G65790.1	50.769	130	60	1	373	498	691	820	3.88e-33	138
Msa1243620	AT1G65790.1	35.075	134	69	5	819	941	694	820	2.47e-14	78.2
Msa1243620	AT4G23290.1	54.701	117	52	1	373	488	492	608	4.29e-33	137
Msa1243620	AT4G23290.1	47.297	74	39	0	858	931	535	608	6.35e-17	86.3
Msa1243620	AT4G23290.2	54.701	117	52	1	373	488	535	651	5.98e-33	137
Msa1243620	AT4G23290.2	38.596	114	65	3	821	931	540	651	6.12e-17	86.3
Msa1243620	AT4G23180.1	51.240	121	59	0	373	493	520	640	7.05e-33	136
Msa1243620	AT4G23180.1	41.667	84	49	0	853	936	557	640	3.99e-15	80.5
Msa1243620	AT1G65800.1	52.459	122	54	1	373	490	695	816	1.80e-32	136
Msa1243620	AT1G65800.1	37.302	126	61	5	819	933	698	816	2.10e-14	78.2

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Find 190 sgRNAs with CRISPR-Local

Find 760 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACATGGTTTGCAGTAAAA+AGG	0.151981	8_2:-30021214	Msa1243620:intron
AAACGAAAATATATACTTGG+AGG	0.547086	8_2:-30027635	Msa1243620:intron
AAACGAAAATATGTGCTTCA+AGG	0.468674	8_2:-30019369	Msa1243620:intron
AAACTGGGCAACTGATCAAC+AGG	0.450413	8_2:-30028614	Msa1243620:CDS
AAAGGAAATAATGATATTAT+AGG	0.260900	8_2:-30017397	Msa1243620:three_prime_UTR
AAATGGACTCTATTAGAAAC+TGG	0.296765	8_2:-30020419	Msa1243620:CDS
AAATGGACTCTATTAGAAAC+TGG	0.296765	8_2:-30028630	Msa1243620:CDS
AACATGGTTTGTAGTGAAAA+AGG	0.190042	8_2:-30029425	Msa1243620:CDS
AAGAAACCAGCATACTATGG+TGG	0.667087	8_2:-30017681	Msa1243620:CDS
AAGAAACCAGCATACTATGG+TGG	0.667087	8_2:-30025749	Msa1243620:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGTCCAAAGAAAAGATGTT+TGG	-	chr8_2:30017573-30017592	Msa1243620:exon	30.0%
TGTCCAAAGAAAAGATGTTT+GGG	-	chr8_2:30017574-30017593	Msa1243620:exon	30.0%
CTCAAAATAGAATCCAACAT+TGG	-	chr8_2:30017671-30017690	Msa1243620:CDS	30.0%
CAAAATAGAATCCAACATTG+GGG	-	chr8_2:30017673-30017692	Msa1243620:CDS	30.0%
GGTGAAGAGTAAAGAATTAT+TGG	+	chr8_2:30017701-30017720	None:intergenic	30.0%
CAAAACAATCTTCATCCTAT+TGG	+	chr8_2:30017801-30017820	None:intergenic	30.0%
ATAACCAATTCTCTTCTTCT+TGG	+	chr8_2:30017968-30017987	None:intergenic	30.0%
CATTTGGTAAGTTCTTCATT+TGG	+	chr8_2:30018121-30018140	None:intergenic	30.0%
CAAATGAAGAACTTACCAAA+TGG	-	chr8_2:30018119-30018138	Msa1243620:intron	30.0%
AGTTTCTAATAGAGTCCATT+TGG	+	chr8_2:30018137-30018156	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	30017344	30029444	30017344	ID=Msa1243620;Name=Msa1243620
chr8_2	mRNA	30017344	30029444	30017344	ID=Msa1243620-mRNA-1;Parent=Msa1243620;Name=Msa1243620-mRNA-1;_AED=0.23;_eAED=0.24;_QI=0\|0.21\|0.2\|1\|0.64\|0.6\|15\|246\|962
chr8_2	exon	30017344	30017898	30017344	ID=Msa1243620-mRNA-1:exon:10159;Parent=Msa1243620-mRNA-1
chr8_2	exon	30019083	30019282	30019083	ID=Msa1243620-mRNA-1:exon:10158;Parent=Msa1243620-mRNA-1
chr8_2	exon	30019370	30019504	30019370	ID=Msa1243620-mRNA-1:exon:10157;Parent=Msa1243620-mRNA-1
chr8_2	exon	30020051	30020275	30020051	ID=Msa1243620-mRNA-1:exon:10156;Parent=Msa1243620-mRNA-1
chr8_2	exon	30020300	30020518	30020300	ID=Msa1243620-mRNA-1:exon:10155;Parent=Msa1243620-mRNA-1
chr8_2	exon	30020906	30021113	30020906	ID=Msa1243620-mRNA-1:exon:10154;Parent=Msa1243620-mRNA-1
chr8_2	exon	30021122	30021221	30021122	ID=Msa1243620-mRNA-1:exon:10153;Parent=Msa1243620-mRNA-1
chr8_2	exon	30025725	30025966	30025725	ID=Msa1243620-mRNA-1:exon:10152;Parent=Msa1243620-mRNA-1
chr8_2	exon	30026060	30026193	30026060	ID=Msa1243620-mRNA-1:exon:10151;Parent=Msa1243620-mRNA-1
chr8_2	exon	30027409	30027552	30027409	ID=Msa1243620-mRNA-1:exon:10150;Parent=Msa1243620-mRNA-1
chr8_2	exon	30027636	30027743	30027636	ID=Msa1243620-mRNA-1:exon:10149;Parent=Msa1243620-mRNA-1
chr8_2	exon	30028274	30028518	30028274	ID=Msa1243620-mRNA-1:exon:10148;Parent=Msa1243620-mRNA-1
chr8_2	exon	30028549	30028796	30028549	ID=Msa1243620-mRNA-1:exon:10147;Parent=Msa1243620-mRNA-1
chr8_2	exon	30029040	30029245	30029040	ID=Msa1243620-mRNA-1:exon:10146;Parent=Msa1243620-mRNA-1
chr8_2	exon	30029279	30029444	30029279	ID=Msa1243620-mRNA-1:exon:10145;Parent=Msa1243620-mRNA-1
chr8_2	CDS	30029279	30029444	30029279	ID=Msa1243620-mRNA-1:cds;Parent=Msa1243620-mRNA-1
chr8_2	CDS	30029040	30029245	30029040	ID=Msa1243620-mRNA-1:cds;Parent=Msa1243620-mRNA-1
chr8_2	CDS	30028549	30028796	30028549	ID=Msa1243620-mRNA-1:cds;Parent=Msa1243620-mRNA-1
chr8_2	CDS	30028274	30028518	30028274	ID=Msa1243620-mRNA-1:cds;Parent=Msa1243620-mRNA-1
chr8_2	CDS	30027636	30027743	30027636	ID=Msa1243620-mRNA-1:cds;Parent=Msa1243620-mRNA-1
chr8_2	CDS	30027409	30027552	30027409	ID=Msa1243620-mRNA-1:cds;Parent=Msa1243620-mRNA-1
chr8_2	CDS	30026060	30026193	30026060	ID=Msa1243620-mRNA-1:cds;Parent=Msa1243620-mRNA-1
chr8_2	CDS	30025725	30025966	30025725	ID=Msa1243620-mRNA-1:cds;Parent=Msa1243620-mRNA-1
chr8_2	CDS	30021122	30021221	30021122	ID=Msa1243620-mRNA-1:cds;Parent=Msa1243620-mRNA-1
chr8_2	CDS	30020906	30021113	30020906	ID=Msa1243620-mRNA-1:cds;Parent=Msa1243620-mRNA-1
chr8_2	CDS	30020300	30020518	30020300	ID=Msa1243620-mRNA-1:cds;Parent=Msa1243620-mRNA-1
chr8_2	CDS	30020051	30020275	30020051	ID=Msa1243620-mRNA-1:cds;Parent=Msa1243620-mRNA-1
chr8_2	CDS	30019370	30019504	30019370	ID=Msa1243620-mRNA-1:cds;Parent=Msa1243620-mRNA-1
chr8_2	CDS	30019083	30019282	30019083	ID=Msa1243620-mRNA-1:cds;Parent=Msa1243620-mRNA-1
chr8_2	CDS	30017590	30017898	30017590	ID=Msa1243620-mRNA-1:cds;Parent=Msa1243620-mRNA-1
chr8_2	three_prime_UTR	30017344	30017589	30017344	ID=Msa1243620-mRNA-1:three_prime_utr;Parent=Msa1243620-mRNA-1

Gene Sequence

>Msa1243620

ATGGTTTGTAGTGAAAAAGGGAAACCTCAACTTTTGTTTCTCTTCACTTACATGACCTTGTGGTGGTGGTGGTGTACTACTATTTGTATCCATGTAAAAGCAGAAAATGATAGTTTGAAGCTAGGAGAAACATTGAATTCCACATCCATGTTGCTTTCTAAACAAAATCCTAAAAGCCTCACTTATCTTCAAATATTTGTCCAAAGAAAAGATGTTTGGGCAGTGTGGTTCGCTAACAGAAACCAACCTGTTGAAATGGGTTCTGCAGTATTAGCACTAAACAACTCTGGTGTACTCAAAATAGAATCCAACATTGGGGAACCAATAATTCTTTACTCTTCACCTCAACCTTTTAACCAAAGCACTATCATACATGGAGAAAAAGTTTACTGGACAAGCGGAGAATTCAAAAACAATAGATTTGAGCATATATCTGCAGAAGACTTTCAGTACAGAATTGTGTCGAATCAGAATGAAGAGTACTTCTCATACACAGCTCCAAATGAAGAACTTACCAAATGGACTCTATTAGAAACTGGGCAACTGATCAACAGGAAAGGAAGTGACATAGCAAGGGCTGATCTATGTTATGGTTATAACACTAATGGAGGGTGCCAAAATGGTGATGTGTTTGAAAGCAAATCAGGTTATCCCAATAATATAATGTTAAATGATATAGGAAATGCTAATTATAGCATAAGTGACTGTCAAACTATTTGTTGGAGAAATTGCAGCTGTTTTGGATTCAAAACTTCTTATGGTAACGGAACTGGTTGTATGTTCTTTCTATCGATGGAAGGTCTTAATATTGCCAGTGGTGGTGATCAGTTATACATTTTAGTAAAGAACACTCGTCACAAAAAAGGTAAACACATATGGATATGGATAAGCACTGCCGTAGCTATTGCTCTACTCATAATCTGTCTTTCCATCTTGTATCGAGCCAAAATGAAACGAAAATATATACTTGGAGTGAATGAAAGAAACAGAATGGTGACTATGCAAGATCTTGAAGGTTCTGGTGGATCCTCTAGCAACAATGATCTAGAAGCTGATTTGAGCAACGGAGATGAATTGAAAGTGTTCAGCTATTCATCAATAATATTAGCTACCAATGAATATGCTATGGAAGGTGTGTTTTCTACAAAGTCTGACGTGTATAGCTTTGGAGTCCTGCTCCTTGAAATTATTAGTGGTGAAAAAAACAATAGCTTCTATAGCGAAGACAGACCACTTAATCTAGTAGGACATACATGGGACCTATGGAAAGAAGGTATGGTTCTTCAGTTGGTGGATCCATCGCTAAATGAGTCGTTTTCTGAAGATGAGGTGCTAAGATGTGTTCATGCTGGTTTATTATGCGTAGAAGAAAATGTGGACGATCGTCCCACCATGTCCAATGTTATATCAATGTTAACAAACAAAATTAAAGTGGATGTTTTGCCTAAGAAACCAGCATACTATGGTGGAACAAGAGTTTTTGAGGAAGAGACTAAAAAGGGAAACCTCAACTTTTGCTTCTCTTCACATACATTTCCTTGTGGTGGTGGTATACTAATATTTCTACCTATGTCAAAGCAGAAATTATTTATACCAGGAGATAAACTGAATGCCGGTGCCACATCCAGGTTGTGTTCTAAACAAGATAGATACTGCATGAATTTTAACCAAAATCCGGATCCTCAAAACCTCACTTATCTCTGCATATTTGGCCAAGGGAAAGATAGTTGGTTAGTATGGATTGCTAACAGAAACCAAAGTGTTGACAAAAATTCAGCAATTCTATCACTAGACTACTCTGACTTTAAGGTCAAAGTTGTGTCTAATGAGGAAGAAGAATACTTCTCATACACAACTCCAAATGAAGACGGTCTTACCAAATGGACTCTATTAGAAACTGGGCAGCTGATAAACAGAGCAGGAAGTGACATAGCAAGGGCTGATATGTGTTATGGGTATAACTCTAATGATGGATGCCAAAAATGGGGTGAAGCAGAGATACCTACTTGCAGGAATGTTGGTTACCCGAATGAAAATATGGTATACCAGATAGCAAATGCTAGTTACGGCATAAGTGATTGCCAAGCTAATTGCTGGAGTAACTGCAGCTGCTTTGGATTCAAAAGCTTGTATGATAATGGAACTGGTTGTGTAATCTTGCAATCAACTAAAGGTCTAAACATCGCAAGCAGTGGTGATCATTACTTGTTTTATATTCTAGTTAAGAACACCAACGATCACAAAGAAATTAAGTTATCCAAGGGAGGTAACAATAACAGCAAGTGGATATGGATTTCCGTTGGAATGGGGACTGTTCTACTCATTATTGGTCTTTCCATTCTGTTTCGAGCCTTAAGGAAACGAAAATATGTGCTTCAAGAGAAGGAACGAATGAGAATTCAGATTGAGATTCAAGATCTTGAAGGTTCTAGGCGATACTCTGATAGCGATGATCTAGAAGGCGATTTAAGCAGCAGAGATGACTTAAAAGTGTTTACCTATTCATCGATAATTTTGGCTACCAATGGCTTTTCTCCTGAAAACAAGCTAGGACAGGGTGGCTTTGGACCTGTTTTTAAGGCATGGGAGCTATGGAAAGAAGGTGTGGTTCTTCAGGTTGTGGATCCATTACTAAATGAGACGTTTTCTGAGGATGAGGTGCTAAGATGTGTTCATATAGGTTTATTATGCGTAGAAGAAAATGCCGATGATCGTCCGACAATGTCCAATGTAATATCAATGTTAACTAACAAAATTAAAGTCGATGTTTTGCCTAAGAAACCAGCATACTATGGTGGAACAAGAGTTTTTGATGAAGAAACGTATGGTGAAGAAGTTGGTGTGTATTCCACGTATGAAAACCCTCATTCACATTTACAATCATTATAG

Protein sequence

>Msa1243620

MVCSEKGKPQLLFLFTYMTLWWWWCTTICIHVKAENDSLKLGETLNSTSMLLSKQNPKSLTYLQIFVQRKDVWAVWFANRNQPVEMGSAVLALNNSGVLKIESNIGEPIILYSSPQPFNQSTIIHGEKVYWTSGEFKNNRFEHISAEDFQYRIVSNQNEEYFSYTAPNEELTKWTLLETGQLINRKGSDIARADLCYGYNTNGGCQNGDVFESKSGYPNNIMLNDIGNANYSISDCQTICWRNCSCFGFKTSYGNGTGCMFFLSMEGLNIASGGDQLYILVKNTRHKKGKHIWIWISTAVAIALLIICLSILYRAKMKRKYILGVNERNRMVTMQDLEGSGGSSSNNDLEADLSNGDELKVFSYSSIILATNEYAMEGVFSTKSDVYSFGVLLLEIISGEKNNSFYSEDRPLNLVGHTWDLWKEGMVLQLVDPSLNESFSEDEVLRCVHAGLLCVEENVDDRPTMSNVISMLTNKIKVDVLPKKPAYYGGTRVFEEETKKGNLNFCFSSHTFPCGGGILIFLPMSKQKLFIPGDKLNAGATSRLCSKQDRYCMNFNQNPDPQNLTYLCIFGQGKDSWLVWIANRNQSVDKNSAILSLDYSDFKVKVVSNEEEEYFSYTTPNEDGLTKWTLLETGQLINRAGSDIARADMCYGYNSNDGCQKWGEAEIPTCRNVGYPNENMVYQIANASYGISDCQANCWSNCSCFGFKSLYDNGTGCVILQSTKGLNIASSGDHYLFYILVKNTNDHKEIKLSKGGNNNSKWIWISVGMGTVLLIIGLSILFRALRKRKYVLQEKERMRIQIEIQDLEGSRRYSDSDDLEGDLSSRDDLKVFTYSSIILATNGFSPENKLGQGGFGPVFKAWELWKEGVVLQVVDPLLNETFSEDEVLRCVHIGLLCVEENADDRPTMSNVISMLTNKIKVDVLPKKPAYYGGTRVFDEETYGEEVGVYSTYENPHSHLQSL*