AlfalfaGEDB | Msa1248000

M. sativa cultivar zhongmu-4 / Msa1248000

KEGG: K04733|K13420
GO: -
PFAM: PF12799.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1248000	XP_024629309.2	26.804	291	133	3	1	211	193	483	1.18e-14	90.5
Msa1248000	XP_024629309.2	31.278	227	152	3	1	225	97	321	3.09e-18	102
Msa1248000	XP_024629309.2	32.609	230	150	4	12	238	132	359	1.58e-17	99.8
Msa1248000	XP_024629309.2	33.810	210	138	1	4	212	76	285	2.67e-22	114
Msa1248000	XP_024629309.2	89.632	598	61	1	1	597	393	990	0.0	1079

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1248000	sp\|C0LGP4\|Y3475_ARATH	37.264	636	346	11	14	597	373	1007	8.48e-124	392
Msa1248000	sp\|C0LGP4\|Y3475_ARATH	30.303	231	159	2	1	230	89	318	2.48e-23	108
Msa1248000	sp\|C0LGP4\|Y3475_ARATH	31.892	185	125	1	31	214	71	255	5.85e-18	91.7
Msa1248000	sp\|C0LGP4\|Y3475_ARATH	32.468	231	124	3	12	211	124	353	6.11e-18	91.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1248000	A0A396GNM3	26.804	291	133	3	1	211	193	483	5.61e-15	90.5
Msa1248000	A0A396GNM3	31.278	227	152	3	1	225	97	321	1.48e-18	102
Msa1248000	A0A396GNM3	32.609	230	150	4	12	238	132	359	7.53e-18	99.8
Msa1248000	A0A396GNM3	33.810	210	138	1	4	212	76	285	1.28e-22	114
Msa1248000	A0A396GNM3	89.632	598	61	1	1	597	393	990	0.0	1079

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1247860	Msa1248000	0.808495	3.011720e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1248000	MtrunA17_Chr8g0363111	89.632	598	61	1	1	597	393	990	0.0	1079
Msa1248000	MtrunA17_Chr8g0363111	33.810	210	138	1	4	212	76	285	2.46e-26	114
Msa1248000	MtrunA17_Chr8g0363111	31.278	227	152	3	1	225	97	321	2.84e-22	102
Msa1248000	MtrunA17_Chr8g0363111	32.609	230	150	4	12	238	132	359	1.45e-21	99.8
Msa1248000	MtrunA17_Chr8g0363111	26.804	291	133	3	1	211	193	483	1.08e-18	90.5
Msa1248000	MtrunA17_Chr8g0362431	84.114	598	94	1	1	597	191	788	0.0	1015
Msa1248000	MtrunA17_Chr8g0362431	27.843	255	148	5	18	242	12	260	4.21e-15	79.0
Msa1248000	MtrunA17_Chr8g0363601	83.612	598	97	1	1	597	183	780	0.0	1012
Msa1248000	MtrunA17_Chr8g0363601	28.571	245	143	4	18	232	4	246	1.93e-17	86.3
Msa1248000	MtrunA17_Chr8g0363241	82.609	598	103	1	1	597	151	748	0.0	1008

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1248000	AT3G47570.1	37.264	636	346	11	14	597	373	1007	8.62e-125	392
Msa1248000	AT3G47570.1	30.303	231	159	2	1	230	89	318	2.52e-24	108
Msa1248000	AT3G47570.1	31.892	185	125	1	31	214	71	255	5.95e-19	91.7
Msa1248000	AT3G47570.1	32.468	231	124	3	12	211	124	353	6.21e-19	91.7
Msa1248000	AT3G47570.1	27.160	162	118	0	50	211	66	227	1.83e-11	67.8
Msa1248000	AT3G47090.1	34.534	611	369	10	6	588	390	997	2.77e-112	359
Msa1248000	AT3G47090.1	31.984	247	155	5	1	239	282	523	2.40e-23	105
Msa1248000	AT3G47090.1	32.961	179	119	1	55	232	95	273	5.14e-20	95.1
Msa1248000	AT3G47090.1	28.182	220	156	2	1	218	89	308	1.42e-16	84.3
Msa1248000	AT3G47580.1	35.257	641	358	10	2	588	362	999	1.59e-111	357

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Find 202 sgRNAs with CRISPR-Local

Find 460 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAATGACCTGGTGCA+AGG	0.608749	8_2:-41390638	None:intergenic
AAAAGTGATTCTAAGGATTT+TGG	0.118040	8_2:-41389766	None:intergenic
AAAATGGTTGACACAACTAA+GGG	0.630517	8_2:-41389985	None:intergenic
AAAGCCTTCTAATGTCTTGT+TGG	0.406579	8_2:+41390515	Msa1248000:CDS
AAAGGAATTGTTTCTGTCAA+AGG	0.461692	8_2:+41391273	Msa1248000:CDS
AAAGGAGATGTGTACAGCTA+TGG	0.488688	8_2:+41391291	Msa1248000:CDS
AAAGTTGTCGTAGCTTAGGA+AGG	0.606853	8_2:-41388895	None:intergenic
AAATCTCGATTTCAAATCAT+TGG	0.327715	8_2:+41390335	Msa1248000:CDS
AACCCAATTGCATACAGAAG+AGG	0.586325	8_2:-41384793	None:intergenic
AACGAAATATATTAAGAAAA+TGG	0.271430	8_2:-41391791	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTTACACAAAATGACGAAG+TGG	+	chr8_2:41384492-41384511	Msa1248000:five_prime_UTR	30.0%
TTTACACAAAATGACGAAGT+GGG	+	chr8_2:41384493-41384512	Msa1248000:five_prime_UTR	30.0%
GATATAGGGATGATATATAG+TGG	-	chr8_2:41384563-41384582	None:intergenic	30.0%
CTTAACGCTAACAAATTTAG+TGG	+	chr8_2:41385077-41385096	Msa1248000:five_prime_UTR	30.0%
AAGGGACTAAAACAAAAAGT+TGG	-	chr8_2:41385313-41385332	None:intergenic	30.0%
CATAATATATGCAAGATCTG+GGG	-	chr8_2:41385897-41385916	None:intergenic	30.0%
GCATAATATATGCAAGATCT+GGG	-	chr8_2:41385898-41385917	None:intergenic	30.0%
TGCATAATATATGCAAGATC+TGG	-	chr8_2:41385899-41385918	None:intergenic	30.0%
AAAAGGATGAATTTGTGAAC+AGG	+	chr8_2:41386196-41386215	Msa1248000:intron	30.0%
GAAATAGAAAAACGAGTTAG+AGG	+	chr8_2:41386519-41386538	Msa1248000:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	41384461	41391800	41384461	ID=Msa1248000;Name=Msa1248000
chr8_2	mRNA	41384461	41391800	41384461	ID=Msa1248000-mRNA-1;Parent=Msa1248000;Name=Msa1248000-mRNA-1;_AED=0.10;_eAED=0.10;_QI=1274\|1\|1\|1\|0.5\|0.66\|3\|186\|597
chr8_2	exon	41384461	41385242	41384461	ID=Msa1248000-mRNA-1:exon:12615;Parent=Msa1248000-mRNA-1
chr8_2	exon	41388716	41390648	41388716	ID=Msa1248000-mRNA-1:exon:12616;Parent=Msa1248000-mRNA-1
chr8_2	exon	41391262	41391800	41391262	ID=Msa1248000-mRNA-1:exon:12617;Parent=Msa1248000-mRNA-1
chr8_2	five_prime_UTR	41384461	41385242	41384461	ID=Msa1248000-mRNA-1:five_prime_utr;Parent=Msa1248000-mRNA-1
chr8_2	five_prime_UTR	41388716	41389207	41388716	ID=Msa1248000-mRNA-1:five_prime_utr;Parent=Msa1248000-mRNA-1
chr8_2	CDS	41389208	41390648	41389208	ID=Msa1248000-mRNA-1:cds;Parent=Msa1248000-mRNA-1
chr8_2	CDS	41391262	41391614	41391262	ID=Msa1248000-mRNA-1:cds;Parent=Msa1248000-mRNA-1
chr8_2	three_prime_UTR	41391615	41391800	41391615	ID=Msa1248000-mRNA-1:three_prime_utr;Parent=Msa1248000-mRNA-1

Gene Sequence

>Msa1248000

ATGAGCAACATGTTTTCTTTTTCTATAAATGATAATAATATAAATGGACCAATACCTGGTACCATCAAAGAGTTACAAAATCTTCAAGTGTTGGATCTTGGAAACAATGGGCTACAAGGATCATTTATTGAGGAGCTTTGTGAACTTCAGAAATTAGGTGAGTTGCATCTAGAAAATAATAAGCTCTCTGGTATTTTACCAACATGCTTGGGAAATATGACTTCTCTTAGAATGATAGACATAAGATGTAATAGTTTGAACTCTAAGATACCTTCCTCTCTTTGGAGTGTCATAGATATCTTAGAGGTAGATTTGTCCTCTAATGCCTTCATTGGTAATCTTCCACCTGAGATTGGGAATTTGAGAGCAATTATACTATTAGACCTATCAAGAAATCAGATTTCAAGCAACATTCCAGCAACCATCAGTTCCTTACAAACATTGCAGATTCTCTCCTTAGCAGATAATAAACTGAATGGATCAATTCCAAAATCACTTGGTCAAATGATAAGCTTGATCTCCTTGGACTTGTCCCAAAATATGTTAACTGGTGTTATTCCAAAATCCTTAGAATCACTTTTGTATCTTCAAAACATCAACTTCTCATATAATAGATTACAAGGAGAGATTCCTGATGGTGGAAATTTCAAAAATTTCATGGCTGAATCTTTCATTCATAATGGAGCCCTTTGTGGCAATCCCCGCCTACATATACCTCTGTGTGTTGAACAAGTTAAAAAAAGGTCAATGGGAAAGTTGTTATTCAAATGCCTCATTCCCTTAGTTGTGTCAACCATTTTGGTTGTTGCATGCATCATACTTTTAACGCATAATAAAAGGAGAAAGATTCAAAATACTCTTGAAAGGGGTTTATCAACTTTAGGAGCTCTAAGAAGAATTTCCTATTACGAACTTGCGCAAGCAACTAATGGATTCAATGAGTGTAATTTACTTGGACGGGGGGGCTTTGGCTCTGTATATCGGGGTAATCTCCACAATGGTGAGATGATTGCAGTTAAAGTAATTGATTTGCAGTCAGAGGCAAAAGCAAAGAGCTTTGATGTAGAATGCAATGCAATGAGAAATCTACGACATCGAAATCTTGTTAAGATTATTTGTAGTTGTTCAAATCTCGATTTCAAATCATTGGTGATGGAGTTCATGTCAAATGGAAGTGTGGACAAATGGTTATATTCAAATAATTGTTGTTTGAGTTTCTTGCAAAGGCTGAATATAATGATAGATGTTGCATCTGCATTGGAATATCTTCATCATGGTTCTTCAGTACCAGTGGTTCATTGTGATCTAAAGCCTTCTAATGTCTTGTTGGATAAAAATATGGTTGCACATGTTAGCGATTTTGGTATTGCCAAGCTCATCGATGAAGGACAATCTGAATGTCATACACAAACTTTGGCTACTATTGGATACCTTGCACCAGAGTATGGATCTAAAGGAATTGTTTCTGTCAAAGGAGATGTGTACAGCTATGGGATTATGCTAATGGAAATCTTTACAAGAAGAAAGCCAACAGATGATATGTTTGTTGCTGAACTGAACTTGAAGACATGGATCAGTGGATCACTGCCTAATTCAATTCTGGAGGTCATGGATTCAAATTTAGTCCAACAAATTGGAGAACAAATGGATGACATATTGACTCACATGTCATCTATTTTTGGTTTAGCCCTCTGTTGTTGTGAAGATTTACCTAAAGCAAGAATTAATATGGCAGATGTTATTACGTCGCTTATCAAAATCAAGACTTTGGTTGTTAGCGCAAACAAGGTCTAG

Protein sequence

>Msa1248000

MSNMFSFSINDNNINGPIPGTIKELQNLQVLDLGNNGLQGSFIEELCELQKLGELHLENNKLSGILPTCLGNMTSLRMIDIRCNSLNSKIPSSLWSVIDILEVDLSSNAFIGNLPPEIGNLRAIILLDLSRNQISSNIPATISSLQTLQILSLADNKLNGSIPKSLGQMISLISLDLSQNMLTGVIPKSLESLLYLQNINFSYNRLQGEIPDGGNFKNFMAESFIHNGALCGNPRLHIPLCVEQVKKRSMGKLLFKCLIPLVVSTILVVACIILLTHNKRRKIQNTLERGLSTLGALRRISYYELAQATNGFNECNLLGRGGFGSVYRGNLHNGEMIAVKVIDLQSEAKAKSFDVECNAMRNLRHRNLVKIICSCSNLDFKSLVMEFMSNGSVDKWLYSNNCCLSFLQRLNIMIDVASALEYLHHGSSVPVVHCDLKPSNVLLDKNMVAHVSDFGIAKLIDEGQSECHTQTLATIGYLAPEYGSKGIVSVKGDVYSYGIMLMEIFTRRKPTDDMFVAELNLKTWISGSLPNSILEVMDSNLVQQIGEQMDDILTHMSSIFGLALCCCEDLPKARINMADVITSLIKIKTLVVSANKV*