AlfalfaGEDB | Msa1278600

M. sativa cultivar zhongmu-4 / Msa1278600

KEGG: K04371|K04464|K20600
GO: GO:0000003 GO:0000165 GO:0000226 GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004707 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0006971 GO:0006972 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009504 GO:0009555 GO:0009605 GO:0009607 GO:0009611 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009733 GO:0009737 GO:0009751 GO:0009753 GO:0009755 GO:0009814 GO:0009861 GO:0009862 GO:0009863 GO:0009867 GO:0009868 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0014070 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0023014 GO:0023052 GO:0030865 GO:0031122 GO:0032260 GO:0032501 GO:0032502 GO:0032504 GO:0032506 GO:0032870 GO:0033206 GO:0033993 GO:0035556 GO:0035690 GO:0035821 GO:0036211 GO:0042221 GO:0042493 GO:0042539 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043622 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045087 GO:0046677 GO:0048229 GO:0048232 GO:0048285 GO:0048609 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051301 GO:0051321 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052306 GO:0052308 GO:0052552 GO:0052553 GO:0052564 GO:0052572 GO:0060255 GO:0061640 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071840 GO:0075136 GO:0080026 GO:0097305 GO:0097435 GO:0098542 GO:0140013 GO:0140096 GO:1901564 GO:1901698 GO:1901700 GO:1901701 GO:1902410 GO:1903046 GO:1903047
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1278600	sp\|Q40353\|MMK2_MEDSA	98.922	371	4	0	1	371	1	371	0.0	764

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1278600	A0A072TMU3	98.652	371	5	0	1	371	1	371	0.0	766

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1177790	Msa1278600	0.961254	1.726257e-119	-8.615850e-47
Msa1237490	Msa1278600	0.801129	1.041447e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1278600	MtrunA17_Chr8g0348481	98.652	371	5	0	1	371	1	371	0.0	766
Msa1278600	MtrunA17_Chr7g0248461	87.601	371	45	1	1	370	1	371	0.0	695
Msa1278600	MtrunA17_Chr8g0387401	81.267	363	68	0	6	368	21	383	0.0	638
Msa1278600	MtrunA17_Chr5g0397261	79.357	373	73	1	1	369	1	373	0.0	632
Msa1278600	MtrunA17_Chr4g0030231	75.069	361	90	0	4	364	7	367	0.0	586
Msa1278600	MtrunA17_Chr4g0045841	71.588	359	101	1	9	367	27	384	0.0	563
Msa1278600	MtrunA17_Chr4g0030151	66.011	356	121	0	12	367	14	369	0.0	537
Msa1278600	MtrunA17_Chr3g0141871	57.423	357	147	4	12	367	10	362	1.49e-153	437
Msa1278600	MtrunA17_Chr8g0378831	54.624	346	154	3	23	367	19	362	4.61e-140	402
Msa1278600	MtrunA17_Chr3g0102571	44.529	393	165	6	23	365	19	408	7.65e-120	353

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa1278600	AT4G01370.1	84.140	372	53	1	1	366	1	372	677
Msa1278600	AT1G01560.2	82.114	369	63	1	1	366	1	369	642
Msa1278600	AT4G11330.1	76.344	372	84	1	3	370	5	376	621
Msa1278600	AT2G46070.3	79.609	358	73	0	10	367	48	405	613
Msa1278600	AT2G46070.1	79.609	358	73	0	10	367	14	371	613
Msa1278600	AT1G07880.2	73.654	353	93	0	12	364	8	360	568
Msa1278600	AT1G01560.3	82.043	323	53	2	1	318	1	323	554
Msa1278600	AT2G46070.2	80.323	310	61	0	10	319	14	323	534
Msa1278600	AT2G43790.1	69.081	359	110	1	9	367	35	392	534
Msa1278600	AT3G45640.1	64.789	355	125	0	12	366	13	367	525

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Find 103 sgRNAs with CRISPR-Local

Find 304 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTCAAAACTGAAAGGTC+TGG	0.503104	8_3:-14998393	None:intergenic
AAATACCTTCCACCATGAGT+TGG	0.623774	8_3:-14993631	None:intergenic
AACAGCACCTGGAGACATGT+TGG	0.529175	8_3:-14998052	None:intergenic
AACTGACAAGTACAATATAA+TGG	0.404291	8_3:-14998736	None:intergenic
AAGAAGTTTGATTTCCCGTA+AGG	0.519538	8_3:-14995168	None:intergenic
AAGATATCAAGGAACTCATA+TGG	0.431117	8_3:+14998438	Msa1278600:CDS
AATACAAAATCTCAATTAAT+TGG	0.244752	8_3:-14998491	None:intergenic
AATCAAACTTCTTCGCCATA+TGG	0.405841	8_3:+14995178	Msa1278600:CDS
AATCCAAGGCTGGCATCATC+AGG	0.445992	8_3:-14997952	None:intergenic
AATGACAGATGATCATTGTC+GGG	0.511642	8_3:+14995442	Msa1278600:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTTCCTTTCTTCTTGCAA+TGG	-	chr8_3:14993263-14993282	None:intergenic	30.0%
TAGAAAAGGAGAAGACTTTA+AGG	-	chr8_3:14993476-14993495	None:intergenic	30.0%
CTGAAACATCCTTTCTATAT+TGG	+	chr8_3:14993498-14993517	Msa1278600:five_prime_UTR	30.0%
TTAGTGTAAAGGGTAGTTTA+TGG	+	chr8_3:14993818-14993837	Msa1278600:intron	30.0%
ATTTGGATTTAAGCATGAAG+TGG	+	chr8_3:14994068-14994087	Msa1278600:intron	30.0%
TTTGGATTTAAGCATGAAGT+GGG	+	chr8_3:14994069-14994088	Msa1278600:intron	30.0%
GAGGTTATGAAAACAACTTA+TGG	+	chr8_3:14994176-14994195	Msa1278600:intron	30.0%
TCCCAATCTAATTGAAACAA+TGG	-	chr8_3:14994588-14994607	None:intergenic	30.0%
AACAGTTAAATAAATCCCAC+AGG	-	chr8_3:14994642-14994661	None:intergenic	30.0%
TAGTACTAAATGTGCAAAAC+TGG	-	chr8_3:14994666-14994685	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	14993260	14998806	14993260	ID=Msa1278600;Name=Msa1278600
chr8_3	mRNA	14993260	14998806	14993260	ID=Msa1278600-mRNA-1;Parent=Msa1278600;Name=Msa1278600-mRNA-1;_AED=0.08;_eAED=0.08;_QI=332\|1\|1\|1\|0.8\|0.83\|6\|304\|371
chr8_3	exon	14993260	14993746	14993260	ID=Msa1278600-mRNA-1:exon:5829;Parent=Msa1278600-mRNA-1
chr8_3	exon	14995082	14995211	14995082	ID=Msa1278600-mRNA-1:exon:5830;Parent=Msa1278600-mRNA-1
chr8_3	exon	14995326	14995463	14995326	ID=Msa1278600-mRNA-1:exon:5831;Parent=Msa1278600-mRNA-1
chr8_3	exon	14997065	14997397	14997065	ID=Msa1278600-mRNA-1:exon:5832;Parent=Msa1278600-mRNA-1
chr8_3	exon	14997940	14998123	14997940	ID=Msa1278600-mRNA-1:exon:5833;Parent=Msa1278600-mRNA-1
chr8_3	exon	14998327	14998806	14998327	ID=Msa1278600-mRNA-1:exon:5834;Parent=Msa1278600-mRNA-1
chr8_3	five_prime_UTR	14993260	14993591	14993260	ID=Msa1278600-mRNA-1:five_prime_utr;Parent=Msa1278600-mRNA-1
chr8_3	CDS	14993592	14993746	14993592	ID=Msa1278600-mRNA-1:cds;Parent=Msa1278600-mRNA-1
chr8_3	CDS	14995082	14995211	14995082	ID=Msa1278600-mRNA-1:cds;Parent=Msa1278600-mRNA-1
chr8_3	CDS	14995326	14995463	14995326	ID=Msa1278600-mRNA-1:cds;Parent=Msa1278600-mRNA-1
chr8_3	CDS	14997065	14997397	14997065	ID=Msa1278600-mRNA-1:cds;Parent=Msa1278600-mRNA-1
chr8_3	CDS	14997940	14998123	14997940	ID=Msa1278600-mRNA-1:cds;Parent=Msa1278600-mRNA-1
chr8_3	CDS	14998327	14998502	14998327	ID=Msa1278600-mRNA-1:cds;Parent=Msa1278600-mRNA-1
chr8_3	three_prime_UTR	14998503	14998806	14998503	ID=Msa1278600-mRNA-1:three_prime_utr;Parent=Msa1278600-mRNA-1

Gene Sequence

>Msa1278600

ATGTCTGTTGAATCAGCTGAGAATAACATCAGAGGAGTACCAACTCATGGTGGAAGGTATTTGCAGTACAATATCTATGGTAACCTCTTCGAGGTTTCTAGGAAGTATGTCCCTCCTATTCGTCCTGTCGGTAGAGGTGCTTATGGTATTGTTTGTGCTGCTGTAAATGCTGAGACACGTGAGGAAGTCGCCATTAAGAAGATTGGCAATGCATTTGACAACAGAATAGATGCTAAAAGGACCTTACGGGAAATCAAACTTCTTCGCCATATGGATCATGAAAATGTGATGTCCATTAAAGATATTATAAGACCTCCACAGAAGGAGAACTTCAATGATGTGTACATTGTTTCTGAGTTAATGGACACAGATCTGCATCAAATAATACGTTCCAATCAACCAATGACAGATGATCATTGTCGGTATTTTGTGTATCAGCTGTTACGAGGACTCAAATACGTACATTCAGCAAATGTTTTGCACCGTGACCTCAAGCCTAGCAATTTGCTACTGAATGCCAATTGTGATCTTAAGATTGGTGACTTTGGTCTTGCCAGAACTACATCAGAAACAGATTTCATGACTGAGTACGTGGTTACTAGGTGGTACAGAGCTCCCGAACTGCTTCTTAATTGTTCAGAATATACAGCAGCCATTGATATATGGTCTGTTGGTTGCATACTTGGTGAAATCGTAACAAGACAACCCCTCTTTCCTGGAAGAGATTATGTTCATCAGCTGAGACTGGTCACAGAGCTGATAGGCTCACCTGATGATGCCAGCCTTGGATTTCTACGCAGTGAGAATGCTCGTAGATACGTAAGACAACTTCCACAATATCCAAAACAAAATTTTTCCGCTAGGTTTCCCAACATGTCTCCAGGTGCTGTTGATTTGCTAGAGAAGATGCTTATCTTTGATCCAAGCAAGCGCATTAGAGTTGATGAAGCATTGTGCCATCCATACATGGCACCTCTGCATGATATCAATGAGGAACCTGTATGTGCCAGACCTTTCAGTTTTGACTTTGAGGAACCAATGTTCACTGAAGAAGATATCAAGGAACTCATATGGAAAGAGTCTGTGAGGTTCAATCCTGATCCACCAATTAATTGA

Protein sequence

>Msa1278600

MSVESAENNIRGVPTHGGRYLQYNIYGNLFEVSRKYVPPIRPVGRGAYGIVCAAVNAETREEVAIKKIGNAFDNRIDAKRTLREIKLLRHMDHENVMSIKDIIRPPQKENFNDVYIVSELMDTDLHQIIRSNQPMTDDHCRYFVYQLLRGLKYVHSANVLHRDLKPSNLLLNANCDLKIGDFGLARTTSETDFMTEYVVTRWYRAPELLLNCSEYTAAIDIWSVGCILGEIVTRQPLFPGRDYVHQLRLVTELIGSPDDASLGFLRSENARRYVRQLPQYPKQNFSARFPNMSPGAVDLLEKMLIFDPSKRIRVDEALCHPYMAPLHDINEEPVCARPFSFDFEEPMFTEEDIKELIWKESVRFNPDPPIN*