AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1291980

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1291980 MtrunA17_Chr8g0366571 96.654 269 9 0 1 269 1 269 0.0 538
Msa1291980 MtrunA17_Chr5g0411131 75.093 269 67 0 1 269 1 269 2.63e-134 380
Msa1291980 MtrunA17_Chr8g0389221 52.612 268 127 0 1 268 1 268 1.54e-101 297
Msa1291980 MtrunA17_Chr4g0073251 46.360 261 137 2 1 258 1 261 2.63e-78 238
Msa1291980 MtrunA17_Chr4g0030021 45.956 272 141 3 1 266 1 272 4.07e-78 238
Msa1291980 MtrunA17_Chr6g0462061 42.023 257 139 3 3 259 4 250 7.63e-68 211
Msa1291980 MtrunA17_Chr1g0199961 42.045 264 143 3 3 266 4 257 6.74e-67 209
Msa1291980 MtrunA17_Chr8g0386471 35.878 262 162 3 1 259 3 261 6.48e-60 191
Msa1291980 MtrunA17_Chr4g0062101 36.538 260 162 2 3 259 6 265 5.13e-58 186
Msa1291980 MtrunA17_Chr5g0447681 27.451 255 155 6 5 252 50 281 5.17e-31 117
Msa1291980 MtrunA17_Chr2g0307651 28.358 134 96 0 25 158 25 158 2.81e-16 74.7
Msa1291980 MtrunA17_Chr2g0307641 36.250 80 49 1 182 259 3 82 3.05e-15 69.7
Msa1291980 MtrunA17_Chr5g0447671 28.571 126 72 3 120 243 49 158 5.33e-13 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1291980 AT5G46700.1 69.888 269 81 0 1 269 1 269 9.00e-131 371
Msa1291980 AT2G19580.1 54.275 269 122 1 1 268 1 269 3.21e-94 278
Msa1291980 AT3G45600.1 44.656 262 139 3 1 256 1 262 9.52e-80 242
Msa1291980 AT4G28050.1 43.411 258 144 1 4 259 5 262 1.15e-76 234
Msa1291980 AT2G23810.1 41.445 263 151 2 4 263 5 267 6.29e-73 224
Msa1291980 AT4G30430.1 38.931 262 158 1 3 262 4 265 4.91e-72 222
Msa1291980 AT5G60220.1 44.656 262 139 3 1 256 1 262 3.26e-64 204
Msa1291980 AT1G18520.1 39.015 264 153 4 3 259 4 266 6.65e-62 196
Msa1291980 AT3G12090.1 40.945 254 138 4 3 256 4 245 1.01e-58 188
Msa1291980 AT1G63260.2 35.659 258 158 3 1 257 3 253 6.18e-58 186
Msa1291980 AT1G63260.6 35.458 251 157 3 1 247 3 252 8.71e-55 178
Msa1291980 AT1G63260.1 35.458 251 157 3 1 247 3 252 8.71e-55 178
Msa1291980 AT1G63260.3 35.458 251 157 3 1 247 3 252 1.23e-54 178
Msa1291980 AT4G23410.1 37.066 259 152 3 3 261 4 251 2.81e-51 169
Msa1291980 AT1G63260.5 35.484 217 138 2 1 216 3 218 1.10e-48 160
Msa1291980 AT3G45600.2 40.936 171 96 2 1 166 1 171 2.82e-41 141
Msa1291980 AT5G23030.1 29.845 258 176 2 1 256 2 256 1.08e-34 126
Msa1291980 AT1G63260.4 32.584 178 119 1 1 178 3 179 5.02e-34 122
Msa1291980 AT2G03840.1 28.000 225 160 2 1 224 19 242 1.06e-33 124
Msa1291980 AT5G57810.1 24.535 269 167 7 1 247 51 305 2.99e-19 85.9

Find 60 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATCTTCACTTGTGTTAAT+TGG 0.120566 8_3:-47600172 None:intergenic
CAATAATTGGTGCTGGAATT+TGG 0.184709 8_3:+47598694 Msa1291980:CDS
ATAAGAAACAAAATTTATTT+AGG 0.208936 8_3:+47598564 Msa1291980:five_prime_UTR
CAAATTCCAGCACCAATTAT+TGG 0.222813 8_3:-47598693 None:intergenic
GGGTTAGAAGCTCACATAAA+TGG 0.224537 8_3:+47599003 Msa1291980:CDS
TTAATTCTTGTTTATTTGTT+TGG 0.231006 8_3:+47600328 Msa1291980:CDS
TAATTCTTGTTTATTTGTTT+GGG 0.261336 8_3:+47600329 Msa1291980:CDS
TATTCTAAATATGACCTATT+AGG 0.288046 8_3:-47598945 None:intergenic
ACTTCCTATAGAACCTTTCA+TGG 0.294123 8_3:+47598527 Msa1291980:five_prime_UTR
TTCTTTATTATCCGCCAAAA+TGG 0.295600 8_3:+47598614 Msa1291980:exon
CTACTCTCAATCCCAATAAT+TGG 0.304738 8_3:+47598681 Msa1291980:CDS
TTGGTTTCATTGGAGCCTTT+TGG 0.313785 8_3:+47598805 Msa1291980:CDS
GCGAAAGAGCTCCTCAGTTT+TGG 0.315533 8_3:-47600371 None:intergenic
CATTTGTGAACCCAACATAT+TGG 0.323094 8_3:+47600143 Msa1291980:CDS
GAATTAGATTATGAACAATA+TGG 0.331847 8_3:+47600461 Msa1291980:three_prime_UTR
AAATTCCAGCACCAATTATT+GGG 0.333661 8_3:-47598692 None:intergenic
TATCTTCACTTGTGTTAATT+GGG 0.340640 8_3:-47600171 None:intergenic
GTGTATTCGGTTACACTTAG+AGG 0.346299 8_3:+47598906 Msa1291980:CDS
TGCTCACCTATCACCCTTGC+AGG 0.349924 8_3:+47599109 Msa1291980:CDS
CTGGTTTGTTAGCAACTCTT+AGG 0.355155 8_3:+47600257 Msa1291980:CDS
GTTGGTATGGTTGGTTTCAT+TGG 0.355504 8_3:+47598795 Msa1291980:CDS
ATTGTTGCTCAATGCCATTT+TGG 0.358475 8_3:-47598628 None:intergenic
AATTAACACAAGTGAAGATA+TGG 0.398975 8_3:+47600174 Msa1291980:CDS
ATGGGAATATTGAACCTAAT+AGG 0.401642 8_3:+47598931 Msa1291980:CDS
GATTTACCTGCAAGGGTGAT+AGG 0.408328 8_3:-47599115 None:intergenic
AAATGATCACTGGCCATTGT+AGG 0.408428 8_3:-47598752 None:intergenic
TAAAGAAATCTTGAGCAATT+CGG 0.414307 8_3:-47599085 None:intergenic
TATACCATGAAAGGTTCTAT+AGG 0.434823 8_3:-47598531 None:intergenic
TTGGGCTAATCCAATATGTT+GGG 0.437117 8_3:-47600153 None:intergenic
GGTTAGAAGCTCACATAAAT+GGG 0.454130 8_3:+47599004 Msa1291980:CDS
AAGATATGGATTGCATGAAA+TGG 0.463820 8_3:+47600188 Msa1291980:CDS
ATTGGGCTAATCCAATATGT+TGG 0.465885 8_3:-47600154 None:intergenic
AATTGTGACTCATGCAAAGC+TGG 0.472474 8_3:+47600238 Msa1291980:CDS
TCAATCCCAATAATTGGTGC+TGG 0.477893 8_3:+47598687 Msa1291980:CDS
AGGTAGAATATGAGAAGCAT+TGG 0.498572 8_3:-47598834 None:intergenic
GCATTGAGCAACAATGTGAT+AGG 0.498985 8_3:+47598636 Msa1291980:CDS
TTGGCGGATAATAAAGAAAG+AGG 0.504921 8_3:-47598609 None:intergenic
AATTAGATTATGAACAATAT+GGG 0.514102 8_3:+47600462 Msa1291980:three_prime_UTR
AAGCATTGGGATTCTCCAAA+AGG 0.516728 8_3:-47598820 None:intergenic
TGTGATATTAATAGTTGCAT+TGG 0.522593 8_3:+47600300 Msa1291980:CDS
GGTAGAATATGAGAAGCATT+GGG 0.553523 8_3:-47598833 None:intergenic
TGGCGGATAATAAAGAAAGA+GGG 0.554953 8_3:-47598608 None:intergenic
AGTATCCCTAAAATGATCAC+TGG 0.555016 8_3:-47598762 None:intergenic
TAAGATATATATACCATGAA+AGG 0.557905 8_3:-47598540 None:intergenic
TCGGTTACACTTAGAGGACA+TGG 0.562986 8_3:+47598912 Msa1291980:CDS
CTCTTTCGCAAATACAAGCA+AGG 0.579124 8_3:+47600385 Msa1291980:CDS
ATAAAGTTATGTACAATGTG+TGG 0.582493 8_3:+47600532 Msa1291980:three_prime_UTR
TAGCAACTCTTAGGATAGAG+TGG 0.584061 8_3:+47600266 Msa1291980:CDS
TGCAACTATTAATATCACAT+TGG 0.593272 8_3:-47600296 None:intergenic
AGAAGCTCACATAAATGGGA+TGG 0.594740 8_3:+47599008 Msa1291980:CDS
TGCAAACCACCAACAAAATG+TGG 0.597359 8_3:+47600115 Msa1291980:CDS
GATTCTTATGAATCCATCCA+CGG 0.612749 8_3:+47600573 Msa1291980:three_prime_UTR
ACTAGTGGATTTACCTGCAA+GGG 0.618595 8_3:-47599122 None:intergenic
GACTAGTGGATTTACCTGCA+AGG 0.628765 8_3:-47599123 None:intergenic
GTCATATTTAGAATATCGTG+TGG 0.633248 8_3:+47598953 Msa1291980:CDS
CGGTTACACTTAGAGGACAT+GGG 0.635110 8_3:+47598913 Msa1291980:CDS
CATGTGTCAAAATCCTACAA+TGG 0.641159 8_3:+47598739 Msa1291980:CDS
AAGAACCCAACAAAACAATG+AGG 0.691690 8_3:-47598872 None:intergenic
CAACTCTTAGGATAGAGTGG+AGG 0.710738 8_3:+47600269 Msa1291980:CDS
TGAGGATGAACATAGCAATG+AGG 0.711779 8_3:-47598854 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAGAAACAAAATTTATTT+AGG + chr8_3:47598564-47598583 Msa1291980:five_prime_UTR 10.0%
!!! ATAATTTTATACGTTATTTT+AGG + chr8_3:47599390-47599409 Msa1291980:intron 10.0%
!! ATAAGAAACAAAATTTATTT+AGG + chr8_3:47598564-47598583 Msa1291980:five_prime_UTR 10.0%
!!! ATAATTTTATACGTTATTTT+AGG + chr8_3:47599390-47599409 Msa1291980:intron 10.0%
!! TGATTTAGTAAATTAATGAT+TGG + chr8_3:47599220-47599239 Msa1291980:intron 15.0%
!!! AATTATGTTTTATGATGAAA+TGG + chr8_3:47599311-47599330 Msa1291980:intron 15.0%
!! TAGGATTAATTAATAATTAG+TGG - chr8_3:47599920-47599939 None:intergenic 15.0%
!!! TAAAGATGAATGATATATTT+TGG - chr8_3:47600023-47600042 None:intergenic 15.0%
!! TAAAAAAAAAGAAAAGGAAA+GGG - chr8_3:47600056-47600075 None:intergenic 15.0%
!! TTAAAAAAAAAGAAAAGGAA+AGG - chr8_3:47600057-47600076 None:intergenic 15.0%
!! AGAAGTTAAAAAAAAAGAAA+AGG - chr8_3:47600062-47600081 None:intergenic 15.0%
!!! TTAATTCTTGTTTATTTGTT+TGG + chr8_3:47600328-47600347 Msa1291980:CDS 15.0%
!!! TAATTCTTGTTTATTTGTTT+GGG + chr8_3:47600329-47600348 Msa1291980:CDS 15.0%
!!! TTTTTATATGTTGAAATTCT+AGG + chr8_3:47600437-47600456 Msa1291980:three_prime_UTR 15.0%
!! AATTAGATTATGAACAATAT+GGG + chr8_3:47600462-47600481 Msa1291980:three_prime_UTR 15.0%
!! TGATTTAGTAAATTAATGAT+TGG + chr8_3:47599220-47599239 Msa1291980:intron 15.0%
!!! AATTATGTTTTATGATGAAA+TGG + chr8_3:47599311-47599330 Msa1291980:intron 15.0%
!! TAGGATTAATTAATAATTAG+TGG - chr8_3:47599920-47599939 None:intergenic 15.0%
!!! TAAAGATGAATGATATATTT+TGG - chr8_3:47600023-47600042 None:intergenic 15.0%
!! TAAAAAAAAAGAAAAGGAAA+GGG - chr8_3:47600056-47600075 None:intergenic 15.0%
!! TTAAAAAAAAAGAAAAGGAA+AGG - chr8_3:47600057-47600076 None:intergenic 15.0%
!! AGAAGTTAAAAAAAAAGAAA+AGG - chr8_3:47600062-47600081 None:intergenic 15.0%
!!! TTAATTCTTGTTTATTTGTT+TGG + chr8_3:47600328-47600347 Msa1291980:CDS 15.0%
!!! TAATTCTTGTTTATTTGTTT+GGG + chr8_3:47600329-47600348 Msa1291980:CDS 15.0%
!!! TTTTTATATGTTGAAATTCT+AGG + chr8_3:47600437-47600456 Msa1291980:three_prime_UTR 15.0%
!! AATTAGATTATGAACAATAT+GGG + chr8_3:47600462-47600481 Msa1291980:three_prime_UTR 15.0%
!! TAAGATATATATACCATGAA+AGG - chr8_3:47598543-47598562 None:intergenic 20.0%
!!! ACTAATTTTAATTGTTGGTA+TGG + chr8_3:47598782-47598801 Msa1291980:CDS 20.0%
!! TATTCTAAATATGACCTATT+AGG - chr8_3:47598948-47598967 None:intergenic 20.0%
!! AAACAAATCAAGTAGTATAA+TGG - chr8_3:47599148-47599167 None:intergenic 20.0%
!!! TTTAAGAGATTTTGAAATCA+TGG - chr8_3:47599341-47599360 None:intergenic 20.0%
!! AAAATTATAGTATGCAATTG+TGG - chr8_3:47599378-47599397 None:intergenic 20.0%
!!! ACATTGTTTTAAAAAGCTTA+CGG + chr8_3:47599477-47599496 Msa1291980:intron 20.0%
!!! AAAGAAGTACAAAATTGATA+TGG - chr8_3:47599724-47599743 None:intergenic 20.0%
!!! GAGATTTTTTTTAAGACTAT+AGG - chr8_3:47599763-47599782 None:intergenic 20.0%
!!! TACAATAAATCTGAGTTTTT+AGG - chr8_3:47599939-47599958 None:intergenic 20.0%
!! AATATGTACTAACATATATG+GGG - chr8_3:47599993-47600012 None:intergenic 20.0%
!! GAATATGTACTAACATATAT+GGG - chr8_3:47599994-47600013 None:intergenic 20.0%
!! AGAATATGTACTAACATATA+TGG - chr8_3:47599995-47600014 None:intergenic 20.0%
!! GAATTAGATTATGAACAATA+TGG + chr8_3:47600461-47600480 Msa1291980:three_prime_UTR 20.0%
!! TAAGATATATATACCATGAA+AGG - chr8_3:47598543-47598562 None:intergenic 20.0%
!!! ACTAATTTTAATTGTTGGTA+TGG + chr8_3:47598782-47598801 Msa1291980:CDS 20.0%
!! TATTCTAAATATGACCTATT+AGG - chr8_3:47598948-47598967 None:intergenic 20.0%
!! AAACAAATCAAGTAGTATAA+TGG - chr8_3:47599148-47599167 None:intergenic 20.0%
!!! TTTAAGAGATTTTGAAATCA+TGG - chr8_3:47599341-47599360 None:intergenic 20.0%
!! AAAATTATAGTATGCAATTG+TGG - chr8_3:47599378-47599397 None:intergenic 20.0%
!!! ACATTGTTTTAAAAAGCTTA+CGG + chr8_3:47599477-47599496 Msa1291980:intron 20.0%
!!! AAAGAAGTACAAAATTGATA+TGG - chr8_3:47599724-47599743 None:intergenic 20.0%
!!! GAGATTTTTTTTAAGACTAT+AGG - chr8_3:47599763-47599782 None:intergenic 20.0%
!!! TACAATAAATCTGAGTTTTT+AGG - chr8_3:47599939-47599958 None:intergenic 20.0%
!! AATATGTACTAACATATATG+GGG - chr8_3:47599993-47600012 None:intergenic 20.0%
!! GAATATGTACTAACATATAT+GGG - chr8_3:47599994-47600013 None:intergenic 20.0%
!! AGAATATGTACTAACATATA+TGG - chr8_3:47599995-47600014 None:intergenic 20.0%
!! GAATTAGATTATGAACAATA+TGG + chr8_3:47600461-47600480 Msa1291980:three_prime_UTR 20.0%
!!! GGGATACTAATTTTAATTGT+TGG + chr8_3:47598777-47598796 Msa1291980:CDS 25.0%
!!! ATTTTAATTGTTGGTATGGT+TGG + chr8_3:47598786-47598805 Msa1291980:CDS 25.0%
!!! TTTGGTGATTTTTGTGTATT+CGG + chr8_3:47598893-47598912 Msa1291980:CDS 25.0%
! TAAAGAAATCTTGAGCAATT+CGG - chr8_3:47599088-47599107 None:intergenic 25.0%
! TTGAAGTATGTTAATTAGGA+GGG - chr8_3:47599172-47599191 None:intergenic 25.0%
! ATTGAAGTATGTTAATTAGG+AGG - chr8_3:47599173-47599192 None:intergenic 25.0%
! TGGATTGAAGTATGTTAATT+AGG - chr8_3:47599176-47599195 None:intergenic 25.0%
! AATAAGAAGATTGCACTAAT+TGG - chr8_3:47599196-47599215 None:intergenic 25.0%
! AATTAGTGCAATCTTCTTAT+TGG + chr8_3:47599195-47599214 Msa1291980:intron 25.0%
!! ATGATGATTTTGAATCACAA+TGG + chr8_3:47599270-47599289 Msa1291980:intron 25.0%
! CTTGAGAAATAGAACTATAT+AGG - chr8_3:47599791-47599810 None:intergenic 25.0%
! AGCAAGAAATGTCATAAAAA+GGG - chr8_3:47599871-47599890 None:intergenic 25.0%
! TATCTTCACTTGTGTTAATT+GGG - chr8_3:47600174-47600193 None:intergenic 25.0%
! ATATCTTCACTTGTGTTAAT+TGG - chr8_3:47600175-47600194 None:intergenic 25.0%
! AATTAACACAAGTGAAGATA+TGG + chr8_3:47600174-47600193 Msa1291980:CDS 25.0%
! TGCAACTATTAATATCACAT+TGG - chr8_3:47600299-47600318 None:intergenic 25.0%
! TGTGATATTAATAGTTGCAT+TGG + chr8_3:47600300-47600319 Msa1291980:CDS 25.0%
! ATAAAGTTATGTACAATGTG+TGG + chr8_3:47600532-47600551 Msa1291980:three_prime_UTR 25.0%
!!! GGGATACTAATTTTAATTGT+TGG + chr8_3:47598777-47598796 Msa1291980:CDS 25.0%
!!! ATTTTAATTGTTGGTATGGT+TGG + chr8_3:47598786-47598805 Msa1291980:CDS 25.0%
!!! TTTGGTGATTTTTGTGTATT+CGG + chr8_3:47598893-47598912 Msa1291980:CDS 25.0%
! TAAAGAAATCTTGAGCAATT+CGG - chr8_3:47599088-47599107 None:intergenic 25.0%
! TTGAAGTATGTTAATTAGGA+GGG - chr8_3:47599172-47599191 None:intergenic 25.0%
! ATTGAAGTATGTTAATTAGG+AGG - chr8_3:47599173-47599192 None:intergenic 25.0%
! TGGATTGAAGTATGTTAATT+AGG - chr8_3:47599176-47599195 None:intergenic 25.0%
! AATAAGAAGATTGCACTAAT+TGG - chr8_3:47599196-47599215 None:intergenic 25.0%
! AATTAGTGCAATCTTCTTAT+TGG + chr8_3:47599195-47599214 Msa1291980:intron 25.0%
!! ATGATGATTTTGAATCACAA+TGG + chr8_3:47599270-47599289 Msa1291980:intron 25.0%
! CTTGAGAAATAGAACTATAT+AGG - chr8_3:47599791-47599810 None:intergenic 25.0%
! AGCAAGAAATGTCATAAAAA+GGG - chr8_3:47599871-47599890 None:intergenic 25.0%
! TATCTTCACTTGTGTTAATT+GGG - chr8_3:47600174-47600193 None:intergenic 25.0%
! ATATCTTCACTTGTGTTAAT+TGG - chr8_3:47600175-47600194 None:intergenic 25.0%
! AATTAACACAAGTGAAGATA+TGG + chr8_3:47600174-47600193 Msa1291980:CDS 25.0%
! TGCAACTATTAATATCACAT+TGG - chr8_3:47600299-47600318 None:intergenic 25.0%
! TGTGATATTAATAGTTGCAT+TGG + chr8_3:47600300-47600319 Msa1291980:CDS 25.0%
! ATAAAGTTATGTACAATGTG+TGG + chr8_3:47600532-47600551 Msa1291980:three_prime_UTR 25.0%
TATACCATGAAAGGTTCTAT+AGG - chr8_3:47598534-47598553 None:intergenic 30.0%
TTCTTTATTATCCGCCAAAA+TGG + chr8_3:47598614-47598633 Msa1291980:exon 30.0%
AAATTCCAGCACCAATTATT+GGG - chr8_3:47598695-47598714 None:intergenic 30.0%
!! TTCATCCTCATTGTTTTGTT+GGG + chr8_3:47598867-47598886 Msa1291980:CDS 30.0%
ATGGGAATATTGAACCTAAT+AGG + chr8_3:47598931-47598950 Msa1291980:CDS 30.0%
! GTCATATTTAGAATATCGTG+TGG + chr8_3:47598953-47598972 Msa1291980:CDS 30.0%
! GTGTGGATGATTTTTCATTT+TGG + chr8_3:47598970-47598989 Msa1291980:CDS 30.0%
! TTAAGACTATAGGACAGTTT+TGG - chr8_3:47599753-47599772 None:intergenic 30.0%
! TTCTATTTCTCAAGAAAGCA+TGG + chr8_3:47599797-47599816 Msa1291980:intron 30.0%
ATGTCATAAAAAGGGAATGT+AGG - chr8_3:47599863-47599882 None:intergenic 30.0%
GAGCAAGAAATGTCATAAAA+AGG - chr8_3:47599872-47599891 None:intergenic 30.0%
TATATGGGGTGAAGAATATA+GGG - chr8_3:47599979-47599998 None:intergenic 30.0%
ATATATGGGGTGAAGAATAT+AGG - chr8_3:47599980-47599999 None:intergenic 30.0%
AGAAAAGGAAAGGGAATAAA+GGG - chr8_3:47600047-47600066 None:intergenic 30.0%
AAGAAAAGGAAAGGGAATAA+AGG - chr8_3:47600048-47600067 None:intergenic 30.0%
! AATGTGTAACCACATTTTGT+TGG - chr8_3:47600127-47600146 None:intergenic 30.0%
! AAGATATGGATTGCATGAAA+TGG + chr8_3:47600188-47600207 Msa1291980:CDS 30.0%
! TATGCATAGAAATTTTTCCG+TGG - chr8_3:47600593-47600612 None:intergenic 30.0%
TATACCATGAAAGGTTCTAT+AGG - chr8_3:47598534-47598553 None:intergenic 30.0%
TTCTTTATTATCCGCCAAAA+TGG + chr8_3:47598614-47598633 Msa1291980:exon 30.0%
AAATTCCAGCACCAATTATT+GGG - chr8_3:47598695-47598714 None:intergenic 30.0%
!! TTCATCCTCATTGTTTTGTT+GGG + chr8_3:47598867-47598886 Msa1291980:CDS 30.0%
ATGGGAATATTGAACCTAAT+AGG + chr8_3:47598931-47598950 Msa1291980:CDS 30.0%
! GTCATATTTAGAATATCGTG+TGG + chr8_3:47598953-47598972 Msa1291980:CDS 30.0%
! GTGTGGATGATTTTTCATTT+TGG + chr8_3:47598970-47598989 Msa1291980:CDS 30.0%
! TTAAGACTATAGGACAGTTT+TGG - chr8_3:47599753-47599772 None:intergenic 30.0%
! TTCTATTTCTCAAGAAAGCA+TGG + chr8_3:47599797-47599816 Msa1291980:intron 30.0%
ATGTCATAAAAAGGGAATGT+AGG - chr8_3:47599863-47599882 None:intergenic 30.0%
GAGCAAGAAATGTCATAAAA+AGG - chr8_3:47599872-47599891 None:intergenic 30.0%
TATATGGGGTGAAGAATATA+GGG - chr8_3:47599979-47599998 None:intergenic 30.0%
ATATATGGGGTGAAGAATAT+AGG - chr8_3:47599980-47599999 None:intergenic 30.0%
AGAAAAGGAAAGGGAATAAA+GGG - chr8_3:47600047-47600066 None:intergenic 30.0%
AAGAAAAGGAAAGGGAATAA+AGG - chr8_3:47600048-47600067 None:intergenic 30.0%
! AATGTGTAACCACATTTTGT+TGG - chr8_3:47600127-47600146 None:intergenic 30.0%
! AAGATATGGATTGCATGAAA+TGG + chr8_3:47600188-47600207 Msa1291980:CDS 30.0%
! TATGCATAGAAATTTTTCCG+TGG - chr8_3:47600593-47600612 None:intergenic 30.0%
ACTTCCTATAGAACCTTTCA+TGG + chr8_3:47598527-47598546 Msa1291980:five_prime_UTR 35.0%
! TGGCGGATAATAAAGAAAGA+GGG - chr8_3:47598611-47598630 None:intergenic 35.0%
! TTGGCGGATAATAAAGAAAG+AGG - chr8_3:47598612-47598631 None:intergenic 35.0%
!! ATTGTTGCTCAATGCCATTT+TGG - chr8_3:47598631-47598650 None:intergenic 35.0%
CTACTCTCAATCCCAATAAT+TGG + chr8_3:47598681-47598700 Msa1291980:CDS 35.0%
CAAATTCCAGCACCAATTAT+TGG - chr8_3:47598696-47598715 None:intergenic 35.0%
!! CAATAATTGGTGCTGGAATT+TGG + chr8_3:47598694-47598713 Msa1291980:CDS 35.0%
CATGTGTCAAAATCCTACAA+TGG + chr8_3:47598739-47598758 Msa1291980:CDS 35.0%
! AATGGCCAGTGATCATTTTA+GGG + chr8_3:47598757-47598776 Msa1291980:CDS 35.0%
AGTATCCCTAAAATGATCAC+TGG - chr8_3:47598765-47598784 None:intergenic 35.0%
!! GGTAGAATATGAGAAGCATT+GGG - chr8_3:47598836-47598855 None:intergenic 35.0%
! AGGTAGAATATGAGAAGCAT+TGG - chr8_3:47598837-47598856 None:intergenic 35.0%
!! GTTCATCCTCATTGTTTTGT+TGG + chr8_3:47598866-47598885 Msa1291980:CDS 35.0%
AAGAACCCAACAAAACAATG+AGG - chr8_3:47598875-47598894 None:intergenic 35.0%
!! CATTGTTTTGTTGGGTTCTT+TGG + chr8_3:47598875-47598894 Msa1291980:CDS 35.0%
!!! TTTCATTTTGGCTTCGTAGA+AGG + chr8_3:47598982-47599001 Msa1291980:CDS 35.0%
!!! TTCATTTTGGCTTCGTAGAA+GGG + chr8_3:47598983-47599002 Msa1291980:CDS 35.0%
GGTTAGAAGCTCACATAAAT+GGG + chr8_3:47599004-47599023 Msa1291980:CDS 35.0%
!! CAAGTAGTATAATGGACTAG+TGG - chr8_3:47599140-47599159 None:intergenic 35.0%
!!! GTTATTTTAGGCTTTGAGAC+CGG + chr8_3:47599402-47599421 Msa1291980:intron 35.0%
CTCAAACAAACCAATTCAAC+CGG - chr8_3:47599424-47599443 None:intergenic 35.0%
CATTTGTGAACCCAACATAT+TGG + chr8_3:47600143-47600162 Msa1291980:CDS 35.0%
TTGGGCTAATCCAATATGTT+GGG - chr8_3:47600156-47600175 None:intergenic 35.0%
ATTGGGCTAATCCAATATGT+TGG - chr8_3:47600157-47600176 None:intergenic 35.0%
!! TGTTTGGGTGTTTTGCTTTT+AGG + chr8_3:47600344-47600363 Msa1291980:CDS 35.0%
!! TTTTAGGAATGCCAAAACTG+AGG + chr8_3:47600360-47600379 Msa1291980:CDS 35.0%
GATTCTTATGAATCCATCCA+CGG + chr8_3:47600573-47600592 Msa1291980:three_prime_UTR 35.0%
! CATAGAAATTTTTCCGTGGA+TGG - chr8_3:47600589-47600608 None:intergenic 35.0%
ACTTCCTATAGAACCTTTCA+TGG + chr8_3:47598527-47598546 Msa1291980:five_prime_UTR 35.0%
! TGGCGGATAATAAAGAAAGA+GGG - chr8_3:47598611-47598630 None:intergenic 35.0%
! TTGGCGGATAATAAAGAAAG+AGG - chr8_3:47598612-47598631 None:intergenic 35.0%
!! ATTGTTGCTCAATGCCATTT+TGG - chr8_3:47598631-47598650 None:intergenic 35.0%
CTACTCTCAATCCCAATAAT+TGG + chr8_3:47598681-47598700 Msa1291980:CDS 35.0%
CAAATTCCAGCACCAATTAT+TGG - chr8_3:47598696-47598715 None:intergenic 35.0%
!! CAATAATTGGTGCTGGAATT+TGG + chr8_3:47598694-47598713 Msa1291980:CDS 35.0%
CATGTGTCAAAATCCTACAA+TGG + chr8_3:47598739-47598758 Msa1291980:CDS 35.0%
! AATGGCCAGTGATCATTTTA+GGG + chr8_3:47598757-47598776 Msa1291980:CDS 35.0%
AGTATCCCTAAAATGATCAC+TGG - chr8_3:47598765-47598784 None:intergenic 35.0%
!! GGTAGAATATGAGAAGCATT+GGG - chr8_3:47598836-47598855 None:intergenic 35.0%
! AGGTAGAATATGAGAAGCAT+TGG - chr8_3:47598837-47598856 None:intergenic 35.0%
!! GTTCATCCTCATTGTTTTGT+TGG + chr8_3:47598866-47598885 Msa1291980:CDS 35.0%
AAGAACCCAACAAAACAATG+AGG - chr8_3:47598875-47598894 None:intergenic 35.0%
!! CATTGTTTTGTTGGGTTCTT+TGG + chr8_3:47598875-47598894 Msa1291980:CDS 35.0%
!!! TTTCATTTTGGCTTCGTAGA+AGG + chr8_3:47598982-47599001 Msa1291980:CDS 35.0%
!!! TTCATTTTGGCTTCGTAGAA+GGG + chr8_3:47598983-47599002 Msa1291980:CDS 35.0%
GGTTAGAAGCTCACATAAAT+GGG + chr8_3:47599004-47599023 Msa1291980:CDS 35.0%
!! CAAGTAGTATAATGGACTAG+TGG - chr8_3:47599140-47599159 None:intergenic 35.0%
!!! GTTATTTTAGGCTTTGAGAC+CGG + chr8_3:47599402-47599421 Msa1291980:intron 35.0%
CTCAAACAAACCAATTCAAC+CGG - chr8_3:47599424-47599443 None:intergenic 35.0%
CATTTGTGAACCCAACATAT+TGG + chr8_3:47600143-47600162 Msa1291980:CDS 35.0%
TTGGGCTAATCCAATATGTT+GGG - chr8_3:47600156-47600175 None:intergenic 35.0%
ATTGGGCTAATCCAATATGT+TGG - chr8_3:47600157-47600176 None:intergenic 35.0%
!! TGTTTGGGTGTTTTGCTTTT+AGG + chr8_3:47600344-47600363 Msa1291980:CDS 35.0%
!! TTTTAGGAATGCCAAAACTG+AGG + chr8_3:47600360-47600379 Msa1291980:CDS 35.0%
GATTCTTATGAATCCATCCA+CGG + chr8_3:47600573-47600592 Msa1291980:three_prime_UTR 35.0%
! CATAGAAATTTTTCCGTGGA+TGG - chr8_3:47600589-47600608 None:intergenic 35.0%
GCATTGAGCAACAATGTGAT+AGG + chr8_3:47598636-47598655 Msa1291980:CDS 40.0%
TCAATCCCAATAATTGGTGC+TGG + chr8_3:47598687-47598706 Msa1291980:CDS 40.0%
AAATGATCACTGGCCATTGT+AGG - chr8_3:47598755-47598774 None:intergenic 40.0%
! CAATGGCCAGTGATCATTTT+AGG + chr8_3:47598756-47598775 Msa1291980:CDS 40.0%
!! GTTGGTATGGTTGGTTTCAT+TGG + chr8_3:47598795-47598814 Msa1291980:CDS 40.0%
! TTGGTTTCATTGGAGCCTTT+TGG + chr8_3:47598805-47598824 Msa1291980:CDS 40.0%
!! AAGCATTGGGATTCTCCAAA+AGG - chr8_3:47598823-47598842 None:intergenic 40.0%
TGAGGATGAACATAGCAATG+AGG - chr8_3:47598857-47598876 None:intergenic 40.0%
GTGTATTCGGTTACACTTAG+AGG + chr8_3:47598906-47598925 Msa1291980:CDS 40.0%
GGGTTAGAAGCTCACATAAA+TGG + chr8_3:47599003-47599022 Msa1291980:CDS 40.0%
AGAAGCTCACATAAATGGGA+TGG + chr8_3:47599008-47599027 Msa1291980:CDS 40.0%
!! ACTAGTGGATTTACCTGCAA+GGG - chr8_3:47599125-47599144 None:intergenic 40.0%
CTTTCATATCTCATGCAGTC+AGG + chr8_3:47600088-47600107 Msa1291980:intron 40.0%
TGCAAACCACCAACAAAATG+TGG + chr8_3:47600115-47600134 Msa1291980:CDS 40.0%
! GTGTAACCACATTTTGTTGG+TGG - chr8_3:47600124-47600143 None:intergenic 40.0%
AATTGTGACTCATGCAAAGC+TGG + chr8_3:47600238-47600257 Msa1291980:CDS 40.0%
!! CTGGTTTGTTAGCAACTCTT+AGG + chr8_3:47600257-47600276 Msa1291980:CDS 40.0%
TAGCAACTCTTAGGATAGAG+TGG + chr8_3:47600266-47600285 Msa1291980:CDS 40.0%
CTCTTTCGCAAATACAAGCA+AGG + chr8_3:47600385-47600404 Msa1291980:CDS 40.0%
GCATTGAGCAACAATGTGAT+AGG + chr8_3:47598636-47598655 Msa1291980:CDS 40.0%
TCAATCCCAATAATTGGTGC+TGG + chr8_3:47598687-47598706 Msa1291980:CDS 40.0%
AAATGATCACTGGCCATTGT+AGG - chr8_3:47598755-47598774 None:intergenic 40.0%
! CAATGGCCAGTGATCATTTT+AGG + chr8_3:47598756-47598775 Msa1291980:CDS 40.0%
!! GTTGGTATGGTTGGTTTCAT+TGG + chr8_3:47598795-47598814 Msa1291980:CDS 40.0%
! TTGGTTTCATTGGAGCCTTT+TGG + chr8_3:47598805-47598824 Msa1291980:CDS 40.0%
!! AAGCATTGGGATTCTCCAAA+AGG - chr8_3:47598823-47598842 None:intergenic 40.0%
TGAGGATGAACATAGCAATG+AGG - chr8_3:47598857-47598876 None:intergenic 40.0%
GTGTATTCGGTTACACTTAG+AGG + chr8_3:47598906-47598925 Msa1291980:CDS 40.0%
GGGTTAGAAGCTCACATAAA+TGG + chr8_3:47599003-47599022 Msa1291980:CDS 40.0%
AGAAGCTCACATAAATGGGA+TGG + chr8_3:47599008-47599027 Msa1291980:CDS 40.0%
!! ACTAGTGGATTTACCTGCAA+GGG - chr8_3:47599125-47599144 None:intergenic 40.0%
CTTTCATATCTCATGCAGTC+AGG + chr8_3:47600088-47600107 Msa1291980:intron 40.0%
TGCAAACCACCAACAAAATG+TGG + chr8_3:47600115-47600134 Msa1291980:CDS 40.0%
! GTGTAACCACATTTTGTTGG+TGG - chr8_3:47600124-47600143 None:intergenic 40.0%
AATTGTGACTCATGCAAAGC+TGG + chr8_3:47600238-47600257 Msa1291980:CDS 40.0%
!! CTGGTTTGTTAGCAACTCTT+AGG + chr8_3:47600257-47600276 Msa1291980:CDS 40.0%
TAGCAACTCTTAGGATAGAG+TGG + chr8_3:47600266-47600285 Msa1291980:CDS 40.0%
CTCTTTCGCAAATACAAGCA+AGG + chr8_3:47600385-47600404 Msa1291980:CDS 40.0%
!!! GTTGCTCAATGCCATTTTGG+CGG - chr8_3:47598628-47598647 None:intergenic 45.0%
TCGGTTACACTTAGAGGACA+TGG + chr8_3:47598912-47598931 Msa1291980:CDS 45.0%
CGGTTACACTTAGAGGACAT+GGG + chr8_3:47598913-47598932 Msa1291980:CDS 45.0%
GATTTACCTGCAAGGGTGAT+AGG - chr8_3:47599118-47599137 None:intergenic 45.0%
!! GACTAGTGGATTTACCTGCA+AGG - chr8_3:47599126-47599145 None:intergenic 45.0%
CAACTCTTAGGATAGAGTGG+AGG + chr8_3:47600269-47600288 Msa1291980:CDS 45.0%
!!! GTTGCTCAATGCCATTTTGG+CGG - chr8_3:47598628-47598647 None:intergenic 45.0%
TCGGTTACACTTAGAGGACA+TGG + chr8_3:47598912-47598931 Msa1291980:CDS 45.0%
CGGTTACACTTAGAGGACAT+GGG + chr8_3:47598913-47598932 Msa1291980:CDS 45.0%
GATTTACCTGCAAGGGTGAT+AGG - chr8_3:47599118-47599137 None:intergenic 45.0%
!! GACTAGTGGATTTACCTGCA+AGG - chr8_3:47599126-47599145 None:intergenic 45.0%
CAACTCTTAGGATAGAGTGG+AGG + chr8_3:47600269-47600288 Msa1291980:CDS 45.0%
!! GGCTTTGAGACCGGTTGAAT+TGG + chr8_3:47599411-47599430 Msa1291980:intron 50.0%
! GCGAAAGAGCTCCTCAGTTT+TGG - chr8_3:47600374-47600393 None:intergenic 50.0%
!! GGCTTTGAGACCGGTTGAAT+TGG + chr8_3:47599411-47599430 Msa1291980:intron 50.0%
! GCGAAAGAGCTCCTCAGTTT+TGG - chr8_3:47600374-47600393 None:intergenic 50.0%
TGCTCACCTATCACCCTTGC+AGG + chr8_3:47599109-47599128 Msa1291980:CDS 55.0%
TGCTCACCTATCACCCTTGC+AGG + chr8_3:47599109-47599128 Msa1291980:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 47598486 47600631 47598486 ID=Msa1291980;Name=Msa1291980
chr8_3 mRNA 47598486 47600631 47598486 ID=Msa1291980-mRNA-1;Parent=Msa1291980;Name=Msa1291980-mRNA-1;_AED=0.05;_eAED=0.05;_QI=147|1|1|1|1|1|2|214|269
chr8_3 exon 47598486 47599130 47598486 ID=Msa1291980-mRNA-1:exon:13266;Parent=Msa1291980-mRNA-1
chr8_3 exon 47600106 47600631 47600106 ID=Msa1291980-mRNA-1:exon:13267;Parent=Msa1291980-mRNA-1
chr8_3 five_prime_UTR 47598486 47598632 47598486 ID=Msa1291980-mRNA-1:five_prime_utr;Parent=Msa1291980-mRNA-1
chr8_3 CDS 47598633 47599130 47598633 ID=Msa1291980-mRNA-1:cds;Parent=Msa1291980-mRNA-1
chr8_3 CDS 47600106 47600417 47600106 ID=Msa1291980-mRNA-1:cds;Parent=Msa1291980-mRNA-1
chr8_3 three_prime_UTR 47600418 47600631 47600418 ID=Msa1291980-mRNA-1:three_prime_utr;Parent=Msa1291980-mRNA-1
Gene Sequence

>Msa1291980

ATGGCATTGAGCAACAATGTGATAGGAATCATAAACTTCATTGCAGTTCTACTCTCAATCCCAATAATTGGTGCTGGAATTTGGCTATCAACTTTACAAGCAGAATCATGTGTCAAAATCCTACAATGGCCAGTGATCATTTTAGGGATACTAATTTTAATTGTTGGTATGGTTGGTTTCATTGGAGCCTTTTGGAGAATCCCAATGCTTCTCATATTCTACCTCATTGCTATGTTCATCCTCATTGTTTTGTTGGGTTCTTTGGTGATTTTTGTGTATTCGGTTACACTTAGAGGACATGGGAATATTGAACCTAATAGGTCATATTTAGAATATCGTGTGGATGATTTTTCATTTTGGCTTCGTAGAAGGGTTAGAAGCTCACATAAATGGGATGGAATAAAGAATTGTCTTAGTTCATCAAATATGTGTGCTGAGTTGAATCAAAGTTACCGAATTGCTCAAGATTTCTTTAATGCTCACCTATCACCCTTGCAGTCAGGTTGTTGCAAACCACCAACAAAATGTGGTTACACATTTGTGAACCCAACATATTGGATTAGCCCAATTAACACAAGTGAAGATATGGATTGCATGAAATGGAGCAATGATCAAACACAACTTTGCTACAATTGTGACTCATGCAAAGCTGGTTTGTTAGCAACTCTTAGGATAGAGTGGAGGAAAGCCAATGTGATATTAATAGTTGCATTGGTTGCTTTAATTCTTGTTTATTTGTTTGGGTGTTTTGCTTTTAGGAATGCCAAAACTGAGGAGCTCTTTCGCAAATACAAGCAAGGCTACACTTGA
Protein sequence

>Msa1291980

MALSNNVIGIINFIAVLLSIPIIGAGIWLSTLQAESCVKILQWPVIILGILILIVGMVGFIGAFWRIPMLLIFYLIAMFILIVLLGSLVIFVYSVTLRGHGNIEPNRSYLEYRVDDFSFWLRRRVRSSHKWDGIKNCLSSSNMCAELNQSYRIAQDFFNAHLSPLQSGCCKPPTKCGYTFVNPTYWISPINTSEDMDCMKWSNDQTQLCYNCDSCKAGLLATLRIEWRKANVILIVALVALILVYLFGCFAFRNAKTEELFRKYKQGYT*