AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1293640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1293640 MtrunA17_Chr8g0356501 93.966 348 13 4 1 343 1 345 0.0 649
Msa1293640 MtrunA17_Chr2g0310011 59.740 385 76 19 1 343 1 348 1.08e-124 362
Msa1293640 MtrunA17_Chr6g0461641 41.961 255 111 9 29 258 35 277 8.28e-52 176
Msa1293640 MtrunA17_Chr2g0321461 52.353 170 61 1 26 175 35 204 1.78e-51 175
Msa1293640 MtrunA17_Chr4g0042451 55.769 156 54 2 29 171 10 163 3.05e-50 171
Msa1293640 MtrunA17_Chr5g0409731 43.307 254 98 12 13 232 1 242 7.51e-50 168
Msa1293640 MtrunA17_Chr5g0417521 55.414 157 58 1 29 173 8 164 1.17e-49 169
Msa1293640 MtrunA17_Chr2g0290991 51.479 169 69 3 24 180 27 194 5.37e-46 159
Msa1293640 MtrunA17_Chr1g0197171 51.515 165 70 2 15 170 23 186 2.36e-45 156
Msa1293640 MtrunA17_Chr7g0254981 55.263 152 53 5 36 179 50 194 6.75e-45 155
Msa1293640 MtrunA17_Chr2g0291021 60.305 131 50 2 36 164 49 179 8.16e-44 153
Msa1293640 MtrunA17_Chr7g0261911 39.614 207 103 7 36 233 262 455 2.25e-37 139
Msa1293640 MtrunA17_Chr1g0190301 41.520 171 77 5 2 164 148 303 2.00e-35 132
Msa1293640 MtrunA17_Chr7g0274471 44.828 145 78 1 36 180 218 360 4.09e-35 132
Msa1293640 MtrunA17_Chr1g0175091 43.796 137 75 1 32 168 254 388 5.11e-31 121
Msa1293640 MtrunA17_Chr7g0258551 40.741 135 79 1 36 170 212 345 1.06e-29 117
Msa1293640 MtrunA17_Chr7g0242521 43.609 133 63 3 36 161 13 140 9.62e-27 105
Msa1293640 MtrunA17_Chr1g0175071 39.610 154 82 3 26 174 167 314 1.03e-24 102
Msa1293640 MtrunA17_Chr3g0095611 37.190 121 73 2 36 155 19 137 1.63e-16 77.4
Msa1293640 MtrunA17_Chr7g0243081 55.357 56 25 0 37 92 72 127 1.24e-15 77.0
Msa1293640 MtrunA17_Chr6g0479431 56.667 60 26 0 37 96 203 262 1.75e-15 76.6
Msa1293640 MtrunA17_Chr1g0210911 56.452 62 22 1 37 93 23 84 2.18e-15 76.3
Msa1293640 MtrunA17_Chr2g0288601 61.111 54 21 0 37 90 72 125 2.39e-15 72.0
Msa1293640 MtrunA17_Chr1g0162931 62.264 53 20 0 37 89 19 71 3.05e-15 75.1
Msa1293640 MtrunA17_Chr8g0366281 58.929 56 23 0 37 92 174 229 5.11e-15 75.1
Msa1293640 MtrunA17_Chr3g0109981 50.000 72 36 0 37 108 275 346 6.00e-15 75.5
Msa1293640 MtrunA17_Chr5g0410741 58.929 56 23 0 37 92 220 275 6.69e-15 75.1
Msa1293640 MtrunA17_Chr1g0175081 49.091 55 28 0 38 92 24 78 1.03e-14 69.3
Msa1293640 MtrunA17_Chr1g0154261 57.143 56 24 0 37 92 127 182 1.41e-14 73.2
Msa1293640 MtrunA17_Chr8g0379121 53.571 56 26 0 37 92 112 167 1.75e-14 73.9
Msa1293640 MtrunA17_Chr2g0320231 55.357 56 25 0 37 92 98 153 3.05e-14 73.2
Msa1293640 MtrunA17_Chr7g0258531 33.846 130 67 2 36 155 213 333 1.15e-13 71.2
Msa1293640 MtrunA17_Chr4g0031961 53.623 69 30 2 23 90 117 184 2.25e-13 70.1
Msa1293640 MtrunA17_Chr2g0283951 58.000 50 21 0 37 86 90 139 3.05e-13 70.1
Msa1293640 MtrunA17_Chr1g0161431 61.818 55 20 1 36 90 200 253 3.19e-13 70.5
Msa1293640 MtrunA17_Chr1g0198601 41.346 104 51 2 36 129 211 314 3.50e-13 69.7
Msa1293640 MtrunA17_Chr7g0255711 61.818 55 20 1 36 90 91 144 3.96e-13 68.9
Msa1293640 MtrunA17_Chr4g0067981 57.627 59 24 1 32 90 211 268 8.85e-13 69.3
Msa1293640 MtrunA17_Chr2g0294911 54.237 59 26 1 32 90 207 264 1.61e-12 68.6
Msa1293640 MtrunA17_Chr5g0402631 60.000 55 22 0 36 90 214 268 2.44e-12 67.0
Msa1293640 MtrunA17_Chr5g0422621 58.182 55 23 0 36 90 198 252 7.06e-12 65.9
Msa1293640 MtrunA17_Chr4g0009901 52.542 59 27 1 32 90 164 221 7.68e-12 65.1
Msa1293640 MtrunA17_Chr4g0045361 55.172 58 26 0 33 90 271 328 2.93e-11 64.3
Msa1293640 MtrunA17_Chr4g0009981 53.448 58 26 1 32 89 112 168 4.85e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1293640 AT1G79430.2 53.552 366 136 11 4 343 1 358 4.11e-110 325
Msa1293640 AT1G79430.1 46.512 301 128 10 68 343 1 293 3.82e-66 211
Msa1293640 AT3G24120.1 50.000 252 96 7 25 261 30 266 9.60e-66 209
Msa1293640 AT4G13640.1 60.317 189 49 4 26 189 27 214 1.93e-65 209
Msa1293640 AT3G12730.1 60.894 179 58 4 25 191 12 190 6.65e-65 205
Msa1293640 AT3G24120.2 49.412 255 96 8 25 261 30 269 1.64e-63 204
Msa1293640 AT4G13640.2 59.375 192 49 5 26 189 27 217 2.57e-63 203
Msa1293640 AT3G04030.3 58.580 169 55 3 25 178 34 202 7.41e-56 187
Msa1293640 AT5G18240.1 54.082 196 67 7 25 204 34 222 6.75e-55 185
Msa1293640 AT5G18240.4 54.082 196 67 7 25 204 34 222 6.75e-55 185
Msa1293640 AT3G04030.2 57.396 169 56 4 25 178 34 201 9.93e-53 179
Msa1293640 AT5G18240.5 53.061 196 67 8 25 204 34 220 1.36e-51 176
Msa1293640 AT3G04030.1 55.621 169 54 4 25 178 34 196 3.52e-49 169
Msa1293640 AT5G18240.2 51.531 196 66 8 25 204 34 216 2.64e-48 167
Msa1293640 AT5G18240.3 51.531 196 66 8 25 204 34 216 2.64e-48 167
Msa1293640 AT1G69580.1 50.581 172 64 3 29 181 27 196 4.88e-48 165
Msa1293640 AT1G69580.2 50.289 173 64 3 29 181 27 197 7.40e-48 164
Msa1293640 AT5G45580.2 41.176 221 101 6 26 217 13 233 9.43e-46 157
Msa1293640 AT5G45580.1 41.475 217 98 6 30 217 1 217 8.96e-45 154
Msa1293640 AT2G01060.1 58.462 130 53 1 36 164 15 144 1.60e-44 154
Msa1293640 AT3G12730.2 52.941 136 52 4 68 191 1 136 8.30e-35 125
Msa1293640 AT4G28610.1 38.824 170 87 3 36 196 225 386 7.39e-34 129
Msa1293640 AT5G29000.1 41.615 161 76 5 13 164 168 319 5.65e-33 126
Msa1293640 AT5G29000.3 41.615 161 76 5 13 164 168 319 5.65e-33 126
Msa1293640 AT5G29000.2 41.615 161 76 5 13 164 211 362 9.50e-33 126
Msa1293640 AT3G13040.3 43.478 161 77 4 22 168 218 378 1.12e-32 126
Msa1293640 AT3G13040.1 43.478 161 77 4 22 168 218 378 1.12e-32 126
Msa1293640 AT3G13040.2 43.478 161 77 4 22 168 218 378 1.12e-32 126
Msa1293640 AT3G04450.4 44.697 132 70 2 36 164 198 329 4.01e-32 124
Msa1293640 AT3G04450.2 44.697 132 70 2 36 164 198 329 4.01e-32 124
Msa1293640 AT3G04450.1 43.939 132 71 1 36 164 238 369 6.02e-32 124
Msa1293640 AT5G06800.1 45.736 129 69 1 36 164 192 319 3.79e-30 118
Msa1293640 AT5G06800.2 44.776 134 68 2 36 164 192 324 1.18e-29 117
Msa1293640 AT3G04030.5 52.381 126 45 3 68 178 1 126 1.30e-28 113
Msa1293640 AT2G20400.1 37.736 159 87 2 36 188 231 383 4.36e-27 110
Msa1293640 AT2G20400.2 37.500 160 87 3 36 188 231 384 7.94e-27 109
Msa1293640 AT3G04030.4 48.413 126 44 4 68 178 1 120 2.08e-22 96.3
Msa1293640 AT5G18240.6 43.791 153 57 8 68 204 1 140 7.86e-22 94.7
Msa1293640 AT2G01060.2 49.474 95 47 1 71 164 1 95 2.56e-21 91.7
Msa1293640 AT2G06020.1 59.375 64 26 0 29 92 79 142 7.56e-21 91.7
Msa1293640 AT5G06800.3 59.649 57 23 0 36 92 192 248 5.04e-18 83.2
Msa1293640 AT5G29000.4 43.434 99 52 3 13 111 211 305 1.70e-17 82.4
Msa1293640 AT3G04450.3 56.140 57 25 0 36 92 238 294 3.62e-17 81.3
Msa1293640 AT1G14600.1 50.685 73 31 1 22 89 4 76 1.72e-16 78.6
Msa1293640 AT2G40260.1 60.714 56 22 0 37 92 83 138 4.38e-16 79.3
Msa1293640 AT2G42660.1 58.929 56 23 0 37 92 51 106 5.92e-16 77.0
Msa1293640 AT4G04605.1 58.491 53 22 0 37 89 15 67 6.63e-16 76.3
Msa1293640 AT2G02060.2 57.627 59 25 0 35 93 28 86 1.47e-15 75.9
Msa1293640 AT2G02060.1 57.627 59 25 0 35 93 28 86 1.47e-15 75.9
Msa1293640 AT1G32240.2 51.429 70 34 0 37 106 214 283 3.14e-15 76.3
Msa1293640 AT1G32240.1 51.429 70 34 0 37 106 214 283 3.69e-15 76.3
Msa1293640 AT5G16560.1 56.667 60 26 0 37 96 220 279 5.52e-15 75.9
Msa1293640 AT2G38300.1 57.143 56 24 0 37 92 55 110 7.11e-15 75.1
Msa1293640 AT5G42630.2 57.143 56 24 0 37 92 106 161 1.53e-14 72.4
Msa1293640 AT5G42630.1 57.143 56 24 0 37 92 106 161 2.16e-14 72.8
Msa1293640 AT4G17695.1 53.333 60 28 0 37 96 165 224 3.84e-14 72.8
Msa1293640 AT3G46640.2 55.714 70 27 2 24 90 129 197 4.50e-14 72.4
Msa1293640 AT3G46640.1 55.714 70 27 2 24 90 129 197 4.50e-14 72.4
Msa1293640 AT3G46640.3 55.714 70 27 2 24 90 129 197 5.57e-14 72.4
Msa1293640 AT4G04580.1 34.513 113 58 3 37 149 15 111 8.44e-14 68.9
Msa1293640 AT5G59570.2 54.286 70 28 2 24 90 126 194 8.69e-14 71.2
Msa1293640 AT5G59570.1 54.286 70 28 2 24 90 126 194 8.69e-14 71.2
Msa1293640 AT5G58080.1 32.394 142 85 3 33 167 191 328 1.71e-13 72.0
Msa1293640 AT5G05090.1 61.818 55 20 1 36 90 81 134 1.73e-13 70.1
Msa1293640 AT3G10760.1 61.818 55 20 1 36 90 105 158 2.53e-13 70.5
Msa1293640 AT4G37180.1 55.172 58 26 0 38 95 212 269 9.63e-13 68.9
Msa1293640 AT2G40970.1 60.000 55 21 1 36 90 105 158 1.03e-12 67.4
Msa1293640 AT4G37180.2 55.172 58 26 0 38 95 219 276 1.07e-12 68.9
Msa1293640 AT3G25790.1 60.000 50 20 0 41 90 201 250 4.34e-12 67.0
Msa1293640 AT3G16857.3 56.364 55 23 1 36 90 167 220 9.82e-12 66.6
Msa1293640 AT3G16857.2 56.364 55 23 1 36 90 237 290 1.17e-11 66.2
Msa1293640 AT1G49560.1 52.941 68 31 1 36 102 192 259 1.18e-11 65.5
Msa1293640 AT3G16857.1 56.364 55 23 1 36 90 237 290 1.23e-11 66.2
Msa1293640 AT2G01760.3 54.545 55 24 1 36 90 135 188 2.39e-11 64.3
Msa1293640 AT5G44190.1 55.556 54 23 1 36 89 147 199 5.28e-11 63.9
Msa1293640 AT5G44190.2 55.556 54 23 1 36 89 147 199 6.52e-11 63.2
Msa1293640 AT1G13300.1 54.545 55 25 0 36 90 181 235 6.89e-11 63.2

Find 84 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTTCCACTTCCATTAAA+AGG 0.118507 8_3:-51098045 None:intergenic
TCATTATCATTCCAAATTAT+TGG 0.120463 8_3:-51098069 None:intergenic
AATGATTCACCTTTGAAATT+AGG 0.181239 8_3:-51098147 None:intergenic
TGATCTTAGGCTTCATGATT+TGG 0.243321 8_3:+51098089 Msa1293640:CDS
ATCATAGTTGAGGGCTTCTT+TGG 0.252047 8_3:-51095892 None:intergenic
GTGGAAAGAGTCCAATAATT+TGG 0.261771 8_3:+51098058 Msa1293640:CDS
AATGAAGAAAATTTGTTTGT+TGG 0.268240 8_3:+51098006 Msa1293640:CDS
ATGAACCACTTATCATTTCT+AGG 0.268481 8_3:-51098378 None:intergenic
GTCCATCGAAGCCGAGGTTT+TGG 0.292562 8_3:-51095976 None:intergenic
TTTGGAATGATAATGATCTT+AGG 0.294293 8_3:+51098076 Msa1293640:CDS
AGAGAATCAAGAACAAATTA+TGG 0.298641 8_3:-51095775 None:intergenic
AAAATACATGCAAAGTATAT+TGG 0.312337 8_3:+51097750 Msa1293640:CDS
ATTCAATGAGCAATCTATTA+AGG 0.315644 8_3:+51096589 Msa1293640:CDS
CAGCATCAACAAAGCGTTCA+TGG 0.328267 8_3:-51096008 None:intergenic
CCACCTTAAGAGCCATCTTC+AGG 0.346223 8_3:+51096400 Msa1293640:CDS
GATCTTAGGCTTCATGATTT+GGG 0.360986 8_3:+51098090 Msa1293640:CDS
AAATGAGGAAAATTATTTAG+AGG 0.363464 8_3:-51097880 None:intergenic
TTTGAACTGCAAAGAAATAC+TGG 0.365692 8_3:+51096741 Msa1293640:CDS
TAATTTCAAAGGTGAATCAT+TGG 0.367186 8_3:+51098149 Msa1293640:CDS
GAAACAAATTCTTTCATGTC+AGG 0.374130 8_3:-51097856 None:intergenic
AAAGCTTATCACACACTTGC+AGG 0.378431 8_3:+51097775 Msa1293640:CDS
ATAGAGAGGCCAACCCTTGA+TGG 0.379955 8_3:+51097958 Msa1293640:CDS
AAAACAATCATGAGGGTTAT+GGG 0.394376 8_3:+51096356 Msa1293640:CDS
GTTGATGCTGTTGCTCAGCT+TGG 0.397802 8_3:+51096021 Msa1293640:CDS
AAACCTGAAGATGGCTCTTA+AGG 0.399926 8_3:-51096403 None:intergenic
TTTCAAGATCTTAACATTTG+TGG 0.407965 8_3:+51097901 Msa1293640:CDS
TTGCTCATTGAATTCCTTGT+GGG 0.416474 8_3:-51096579 None:intergenic
ACAAATTTCAAGGGTATGAA+TGG 0.427658 8_3:+51097820 Msa1293640:CDS
ATTTGTTTGTTGGAAAGAAA+AGG 0.433652 8_3:+51098016 Msa1293640:CDS
AATGAATATTCTTAACCAAA+TGG 0.435132 8_3:-51098306 None:intergenic
GAGCCTCAATCCTCAATTGA+AGG 0.444605 8_3:-51097723 None:intergenic
AGCTGCAGCTACAAATTTCA+AGG 0.446327 8_3:+51097810 Msa1293640:CDS
TTTGATCCATCTGTGAAGCT+TGG 0.458634 8_3:+51098234 Msa1293640:CDS
TTCAAAAGCACCTTCAATTG+AGG 0.460223 8_3:+51097713 Msa1293640:CDS
GGTAGAAGCTCATCACCATT+TGG 0.461218 8_3:+51098291 Msa1293640:CDS
GCTGCCACAGCATTGATCAT+AGG 0.463360 8_3:-51098267 None:intergenic
GATGCTGTTGCTCAGCTTGG+AGG 0.468617 8_3:+51096024 Msa1293640:CDS
TCTTTCCACTTCCATTAAAA+GGG 0.471840 8_3:-51098044 None:intergenic
GATGGCTCTTAAGGTGGTAA+AGG 0.478682 8_3:-51096394 None:intergenic
TTGAAATTAGGAACATCATC+AGG 0.480806 8_3:-51098135 None:intergenic
TGAGAATTCATCATAGTTGA+GGG 0.480954 8_3:-51095901 None:intergenic
ATAGTTGAGGGCTTCTTTGG+AGG 0.483070 8_3:-51095889 None:intergenic
ATCCAAAACCTCGGCTTCGA+TGG 0.484201 8_3:+51095974 Msa1293640:CDS
TAGGCATTATAATTCCATCA+AGG 0.485394 8_3:-51097972 None:intergenic
ATGTGTGTTCAAGGTGACTC+TGG 0.488847 8_3:+51095934 Msa1293640:CDS
AATGATAAGTGGTTCATACT+AGG 0.493171 8_3:+51098384 Msa1293640:three_prime_UTR
GATAGACCCATGTGTGTTCA+AGG 0.494833 8_3:+51095925 Msa1293640:CDS
CATTAATAGAAATTTAGGCT+TGG 0.499746 8_3:+51096548 Msa1293640:intron
AGGCATTATAATTCCATCAA+GGG 0.502907 8_3:-51097971 None:intergenic
TAGTTGAGGGCTTCTTTGGA+GGG 0.504455 8_3:-51095888 None:intergenic
TTTGTGTTCAAACCTGAAGA+TGG 0.506495 8_3:-51096412 None:intergenic
GCACCTTCAATTGAGGATTG+AGG 0.506889 8_3:+51097720 Msa1293640:CDS
GATGATGTTCCTAATTTCAA+AGG 0.507643 8_3:+51098138 Msa1293640:CDS
ATGATCAATGCTGTGGCAGC+TGG 0.512611 8_3:+51098270 Msa1293640:CDS
ATGGCTCTTAAGGTGGTAAA+GGG 0.519319 8_3:-51096393 None:intergenic
ATTATAATTCCATCAAGGGT+TGG 0.522631 8_3:-51097967 None:intergenic
TACAAGAATATAGTGAGAGA+TGG 0.526420 8_3:+51095855 Msa1293640:exon
GGCACCTCGTCTTCTATGAC+TGG 0.530737 8_3:+51096762 Msa1293640:CDS
CACACTTGCAGGTGAAAACA+TGG 0.531497 8_3:+51097786 Msa1293640:CDS
ATGTTAAGATCTTGAAAATG+AGG 0.534145 8_3:-51097895 None:intergenic
ATGAGAATTCATCATAGTTG+AGG 0.541419 8_3:-51095902 None:intergenic
CATGAGGGTTATGGGTGTGA+AGG 0.546666 8_3:+51096364 Msa1293640:CDS
AAACAAATTCTTTCATGTCA+GGG 0.546749 8_3:-51097855 None:intergenic
TTGGATCAGTGGTGAGAACA+AGG 0.553644 8_3:-51095957 None:intergenic
GTTATTCCAAGCTTCACAGA+TGG 0.570892 8_3:-51098240 None:intergenic
TTGGTCCTAGAAATGATAAG+TGG 0.572634 8_3:+51098373 Msa1293640:three_prime_UTR
AATGAGCAATCTATTAAGGA+TGG 0.585044 8_3:+51096593 Msa1293640:CDS
ATTGCTCATTGAATTCCTTG+TGG 0.590023 8_3:-51096580 None:intergenic
ATGAGGGTTATGGGTGTGAA+GGG 0.590465 8_3:+51096365 Msa1293640:CDS
CCTGAAGATGGCTCTTAAGG+TGG 0.594196 8_3:-51096400 None:intergenic
TCGACGGTCCATCGAAGCCG+AGG 0.596295 8_3:-51095982 None:intergenic
AAGGGTATGAATGGAACTCA+AGG 0.599882 8_3:+51097829 Msa1293640:CDS
AGAGTCACCTTGAACACACA+TGG 0.606753 8_3:-51095932 None:intergenic
TGCTCATTGAATTCCTTGTG+GGG 0.607189 8_3:-51096578 None:intergenic
GCTTGGAAAGCAACCCCACA+AGG 0.615422 8_3:+51096565 Msa1293640:CDS
ATCTTCATCACAACATAGAG+AGG 0.631722 8_3:+51097944 Msa1293640:CDS
CAATTGAGGATTGAGGCTCA+AGG 0.648335 8_3:+51097727 Msa1293640:CDS
GCTGCAGCTACAAATTTCAA+GGG 0.661788 8_3:+51097811 Msa1293640:CDS
AAGACTGCATGAACAGCTAG+AGG 0.664015 8_3:+51097249 Msa1293640:CDS
ATTACCTATGATCAATGCTG+TGG 0.664522 8_3:+51098263 Msa1293640:CDS
TCACCACTGATCCAAAACCT+CGG 0.679105 8_3:+51095965 Msa1293640:CDS
AAAGCGTTCATGGAGTTCGA+CGG 0.684910 8_3:-51095998 None:intergenic
GCGACCAGTCATAGAAGACG+AGG 0.697663 8_3:-51096766 None:intergenic
GAGTCACCTTGAACACACAT+GGG 0.714712 8_3:-51095931 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAGAAAATAAAAATACTTA+AGG - chr8_3:51096650-51096669 None:intergenic 10.0%
!! TAAATAAATTAAGAATCAAA+AGG - chr8_3:51096818-51096837 None:intergenic 10.0%
!! AAAAAATTAAATTAAAATGC+AGG - chr8_3:51097050-51097069 None:intergenic 10.0%
!! TCTTCATTATTATTATTATT+AGG - chr8_3:51097994-51098013 None:intergenic 10.0%
!!! TTTTCACTATTAATTATTTA+TGG + chr8_3:51098412-51098431 Msa1293640:three_prime_UTR 10.0%
!! AAAAGAAAGAAAAGAATAAA+AGG - chr8_3:51096071-51096090 None:intergenic 15.0%
!!! TTTTATTCTTTTCTTTCTTT+TGG + chr8_3:51096070-51096089 Msa1293640:intron 15.0%
!! TAAAATCAAAGAACTTAAAA+GGG - chr8_3:51096518-51096537 None:intergenic 15.0%
!! ATAAAATCAAAGAACTTAAA+AGG - chr8_3:51096519-51096538 None:intergenic 15.0%
!!! TTTTGCATTAATAGAAATTT+AGG + chr8_3:51096543-51096562 Msa1293640:intron 15.0%
!!! CAATAAAATTTTTTACGATT+TGG + chr8_3:51097083-51097102 Msa1293640:intron 15.0%
!!! TACTCATCACAATTTTTTTA+GGG - chr8_3:51096211-51096230 None:intergenic 20.0%
!!! TTACTCATCACAATTTTTTT+AGG - chr8_3:51096212-51096231 None:intergenic 20.0%
!! AAGTTGCAAATTATATTACT+AGG - chr8_3:51096278-51096297 None:intergenic 20.0%
!!! TTTCAATATTAGTTTACTTG+TGG - chr8_3:51096857-51096876 None:intergenic 20.0%
!! CATATTATAATGAGAAACTT+TGG + chr8_3:51097122-51097141 Msa1293640:intron 20.0%
!!! AAAGTTAGTTTCTAATTTCT+AGG + chr8_3:51097399-51097418 Msa1293640:intron 20.0%
!! TTTCAATAAAGATTTGAACT+CGG - chr8_3:51097562-51097581 None:intergenic 20.0%
!!! TTAATTTGTAGGTTTATTTG+TGG + chr8_3:51097686-51097705 Msa1293640:intron 20.0%
!! AAAATACATGCAAAGTATAT+TGG + chr8_3:51097750-51097769 Msa1293640:CDS 20.0%
!! AAATGAGGAAAATTATTTAG+AGG - chr8_3:51097883-51097902 None:intergenic 20.0%
!!! AATGAAGAAAATTTGTTTGT+TGG + chr8_3:51098006-51098025 Msa1293640:CDS 20.0%
!! TCATTATCATTCCAAATTAT+TGG - chr8_3:51098072-51098091 None:intergenic 20.0%
!! AATGAATATTCTTAACCAAA+TGG - chr8_3:51098309-51098328 None:intergenic 20.0%
!!! ATGAGATATATTTTTGAGTT+TGG + chr8_3:51098354-51098373 Msa1293640:three_prime_UTR 20.0%
! AGAGAATCAAGAACAAATTA+TGG - chr8_3:51095778-51095797 None:intergenic 25.0%
!!! ACTCATCACAATTTTTTTAG+GGG - chr8_3:51096210-51096229 None:intergenic 25.0%
! CTAAATGAGGTAAAGAAATT+TGG - chr8_3:51096452-51096471 None:intergenic 25.0%
! CATTAATAGAAATTTAGGCT+TGG + chr8_3:51096548-51096567 Msa1293640:intron 25.0%
! ATTCAATGAGCAATCTATTA+AGG + chr8_3:51096589-51096608 Msa1293640:CDS 25.0%
!!! TTTTGTTTTCATGACAGTTT+CGG + chr8_3:51096716-51096735 Msa1293640:intron 25.0%
!! GACTAAAAGTATATAGAAGA+GGG - chr8_3:51096893-51096912 None:intergenic 25.0%
!! AGACTAAAAGTATATAGAAG+AGG - chr8_3:51096894-51096913 None:intergenic 25.0%
! AGTTAGTAGTTATTATATGC+AGG - chr8_3:51097006-51097025 None:intergenic 25.0%
! TTATAATGAGAAACTTTGGT+TGG + chr8_3:51097126-51097145 Msa1293640:intron 25.0%
!! TATATAGAGCTAGTGTTTTA+TGG + chr8_3:51097298-51097317 Msa1293640:intron 25.0%
! AAATAACAAAGTGATTGAGT+TGG + chr8_3:51097497-51097516 Msa1293640:intron 25.0%
! TAATCTATAGTTCAATCAGA+AGG - chr8_3:51097609-51097628 None:intergenic 25.0%
! GATTACTAAAATTTCTCTCT+AGG + chr8_3:51097624-51097643 Msa1293640:intron 25.0%
! GGATTAAGACAAAAAATATG+TGG + chr8_3:51097645-51097664 Msa1293640:intron 25.0%
! AAACAAATTCTTTCATGTCA+GGG - chr8_3:51097858-51097877 None:intergenic 25.0%
! ATGTTAAGATCTTGAAAATG+AGG - chr8_3:51097898-51097917 None:intergenic 25.0%
! TTTCAAGATCTTAACATTTG+TGG + chr8_3:51097901-51097920 Msa1293640:CDS 25.0%
!! ATTTGTTTGTTGGAAAGAAA+AGG + chr8_3:51098016-51098035 Msa1293640:CDS 25.0%
!! TTTGGAATGATAATGATCTT+AGG + chr8_3:51098076-51098095 Msa1293640:CDS 25.0%
!!! AATGATTCACCTTTGAAATT+AGG - chr8_3:51098150-51098169 None:intergenic 25.0%
! TAATTTCAAAGGTGAATCAT+TGG + chr8_3:51098149-51098168 Msa1293640:CDS 25.0%
TACAAGAATATAGTGAGAGA+TGG + chr8_3:51095855-51095874 Msa1293640:exon 30.0%
TGAGAATTCATCATAGTTGA+GGG - chr8_3:51095904-51095923 None:intergenic 30.0%
ATGAGAATTCATCATAGTTG+AGG - chr8_3:51095905-51095924 None:intergenic 30.0%
TCTACAAATTAACACACAAG+AGG - chr8_3:51096326-51096345 None:intergenic 30.0%
! ATAACCCTCATGATTGTTTT+TGG - chr8_3:51096356-51096375 None:intergenic 30.0%
AAAAACAATCATGAGGGTTA+TGG + chr8_3:51096355-51096374 Msa1293640:CDS 30.0%
AAAACAATCATGAGGGTTAT+GGG + chr8_3:51096356-51096375 Msa1293640:CDS 30.0%
TCACAAAAGTGAACTAAATG+AGG - chr8_3:51096465-51096484 None:intergenic 30.0%
TCAAAGAACTTAAAAGGGAA+AGG - chr8_3:51096513-51096532 None:intergenic 30.0%
AATGAGCAATCTATTAAGGA+TGG + chr8_3:51096593-51096612 Msa1293640:CDS 30.0%
TTTGAACTGCAAAGAAATAC+TGG + chr8_3:51096741-51096760 Msa1293640:CDS 30.0%
! ATTAGTTTACTTGTGGTTGA+GGG - chr8_3:51096850-51096869 None:intergenic 30.0%
! TATTAGTTTACTTGTGGTTG+AGG - chr8_3:51096851-51096870 None:intergenic 30.0%
TTAGTTACGGTTACATACAA+TGG - chr8_3:51097181-51097200 None:intergenic 30.0%
AACCGTAACTAAAACTTTGT+TGG + chr8_3:51097189-51097208 Msa1293640:intron 30.0%
!!! CACCAACAAAGTTTTAGTTA+CGG - chr8_3:51097194-51097213 None:intergenic 30.0%
ACACTATACATATGATCTTG+TGG - chr8_3:51097324-51097343 None:intergenic 30.0%
AGATCATATGTATAGTGTTC+AGG + chr8_3:51097326-51097345 Msa1293640:intron 30.0%
! CTTTTCCGAGTATTTTATGT+CGG + chr8_3:51097472-51097491 Msa1293640:intron 30.0%
TTTGACCGACATAAAATACT+CGG - chr8_3:51097480-51097499 None:intergenic 30.0%
!! TTGGTTTGTAAACTTCACTA+AGG + chr8_3:51097516-51097535 Msa1293640:intron 30.0%
AAAATATGTGGTGCAAATGT+TGG + chr8_3:51097657-51097676 Msa1293640:intron 30.0%
! ACAAATTTCAAGGGTATGAA+TGG + chr8_3:51097820-51097839 Msa1293640:CDS 30.0%
GAAACAAATTCTTTCATGTC+AGG - chr8_3:51097859-51097878 None:intergenic 30.0%
ATTATAATTCCATCAAGGGT+TGG - chr8_3:51097970-51097989 None:intergenic 30.0%
AGGCATTATAATTCCATCAA+GGG - chr8_3:51097974-51097993 None:intergenic 30.0%
TAGGCATTATAATTCCATCA+AGG - chr8_3:51097975-51097994 None:intergenic 30.0%
! AAAAGGACTAACCCTTTTAA+TGG + chr8_3:51098033-51098052 Msa1293640:CDS 30.0%
TCTTTCCACTTCCATTAAAA+GGG - chr8_3:51098047-51098066 None:intergenic 30.0%
TTGAAATTAGGAACATCATC+AGG - chr8_3:51098138-51098157 None:intergenic 30.0%
GATGATGTTCCTAATTTCAA+AGG + chr8_3:51098138-51098157 Msa1293640:CDS 30.0%
ATGAACCACTTATCATTTCT+AGG - chr8_3:51098381-51098400 None:intergenic 30.0%
AATGATAAGTGGTTCATACT+AGG + chr8_3:51098384-51098403 Msa1293640:three_prime_UTR 30.0%
CACTCCAAAAACAATCATGA+GGG + chr8_3:51096349-51096368 Msa1293640:CDS 35.0%
TTTGTGTTCAAACCTGAAGA+TGG - chr8_3:51096415-51096434 None:intergenic 35.0%
TTGCTCATTGAATTCCTTGT+GGG - chr8_3:51096582-51096601 None:intergenic 35.0%
ATTGCTCATTGAATTCCTTG+TGG - chr8_3:51096583-51096602 None:intergenic 35.0%
TCTATTAAGGATGGTATGAG+AGG + chr8_3:51096602-51096621 Msa1293640:intron 35.0%
ATGCAGGAAAATACATGTTG+AGG - chr8_3:51097034-51097053 None:intergenic 35.0%
CGTAACTAAAACTTTGTTGG+TGG + chr8_3:51097192-51097211 Msa1293640:intron 35.0%
!!! GCAACATTTTAGGATGCAAA+TGG + chr8_3:51097216-51097235 Msa1293640:intron 35.0%
!!! ACATTTTAGGATGCAAATGG+AGG + chr8_3:51097219-51097238 Msa1293640:intron 35.0%
!! GTTGGAAGTGCTTAATTTGT+AGG + chr8_3:51097675-51097694 Msa1293640:intron 35.0%
TTTGTAGGTTTATTTGTGGC+AGG + chr8_3:51097690-51097709 Msa1293640:intron 35.0%
!! TTCAAAAGCACCTTCAATTG+AGG + chr8_3:51097713-51097732 Msa1293640:CDS 35.0%
ATCTTCATCACAACATAGAG+AGG + chr8_3:51097944-51097963 Msa1293640:CDS 35.0%
! ACTAACCCTTTTAATGGAAG+TGG + chr8_3:51098039-51098058 Msa1293640:CDS 35.0%
CTCTTTCCACTTCCATTAAA+AGG - chr8_3:51098048-51098067 None:intergenic 35.0%
GTGGAAAGAGTCCAATAATT+TGG + chr8_3:51098058-51098077 Msa1293640:CDS 35.0%
TGATCTTAGGCTTCATGATT+TGG + chr8_3:51098089-51098108 Msa1293640:CDS 35.0%
GATCTTAGGCTTCATGATTT+GGG + chr8_3:51098090-51098109 Msa1293640:CDS 35.0%
ATTACCTATGATCAATGCTG+TGG + chr8_3:51098263-51098282 Msa1293640:CDS 35.0%
TTGGTCCTAGAAATGATAAG+TGG + chr8_3:51098373-51098392 Msa1293640:three_prime_UTR 35.0%
ATCATAGTTGAGGGCTTCTT+TGG - chr8_3:51095895-51095914 None:intergenic 40.0%
AGGAGAAGATGAGTACTATC+AGG - chr8_3:51096051-51096070 None:intergenic 40.0%
!!! CCTCATGATTGTTTTTGGAG+TGG - chr8_3:51096351-51096370 None:intergenic 40.0%
CCACTCCAAAAACAATCATG+AGG + chr8_3:51096348-51096367 Msa1293640:CDS 40.0%
ATGGCTCTTAAGGTGGTAAA+GGG - chr8_3:51096396-51096415 None:intergenic 40.0%
AAACCTGAAGATGGCTCTTA+AGG - chr8_3:51096406-51096425 None:intergenic 40.0%
TGCTCATTGAATTCCTTGTG+GGG - chr8_3:51096581-51096600 None:intergenic 40.0%
!!! TGTTGGTGGTGCAACATTTT+AGG + chr8_3:51097206-51097225 Msa1293640:intron 40.0%
AAAGCTTATCACACACTTGC+AGG + chr8_3:51097775-51097794 Msa1293640:CDS 40.0%
AGCTGCAGCTACAAATTTCA+AGG + chr8_3:51097810-51097829 Msa1293640:CDS 40.0%
GCTGCAGCTACAAATTTCAA+GGG + chr8_3:51097811-51097830 Msa1293640:CDS 40.0%
! AAGGGTATGAATGGAACTCA+AGG + chr8_3:51097829-51097848 Msa1293640:CDS 40.0%
TTTGATCCATCTGTGAAGCT+TGG + chr8_3:51098234-51098253 Msa1293640:CDS 40.0%
GTTATTCCAAGCTTCACAGA+TGG - chr8_3:51098243-51098262 None:intergenic 40.0%
TAGTTGAGGGCTTCTTTGGA+GGG - chr8_3:51095891-51095910 None:intergenic 45.0%
ATAGTTGAGGGCTTCTTTGG+AGG - chr8_3:51095892-51095911 None:intergenic 45.0%
GATAGACCCATGTGTGTTCA+AGG + chr8_3:51095925-51095944 Msa1293640:CDS 45.0%
GAGTCACCTTGAACACACAT+GGG - chr8_3:51095934-51095953 None:intergenic 45.0%
AGAGTCACCTTGAACACACA+TGG - chr8_3:51095935-51095954 None:intergenic 45.0%
ATGTGTGTTCAAGGTGACTC+TGG + chr8_3:51095934-51095953 Msa1293640:CDS 45.0%
TTGGATCAGTGGTGAGAACA+AGG - chr8_3:51095960-51095979 None:intergenic 45.0%
TCACCACTGATCCAAAACCT+CGG + chr8_3:51095965-51095984 Msa1293640:CDS 45.0%
! AAAGCGTTCATGGAGTTCGA+CGG - chr8_3:51096001-51096020 None:intergenic 45.0%
! CAGCATCAACAAAGCGTTCA+TGG - chr8_3:51096011-51096030 None:intergenic 45.0%
ATGAGGGTTATGGGTGTGAA+GGG + chr8_3:51096365-51096384 Msa1293640:CDS 45.0%
GATGGCTCTTAAGGTGGTAA+AGG - chr8_3:51096397-51096416 None:intergenic 45.0%
AAGACTGCATGAACAGCTAG+AGG + chr8_3:51097249-51097268 Msa1293640:CDS 45.0%
GCACCTTCAATTGAGGATTG+AGG + chr8_3:51097720-51097739 Msa1293640:CDS 45.0%
GAGCCTCAATCCTCAATTGA+AGG - chr8_3:51097726-51097745 None:intergenic 45.0%
CAATTGAGGATTGAGGCTCA+AGG + chr8_3:51097727-51097746 Msa1293640:CDS 45.0%
CACACTTGCAGGTGAAAACA+TGG + chr8_3:51097786-51097805 Msa1293640:CDS 45.0%
GGTAGAAGCTCATCACCATT+TGG + chr8_3:51098291-51098310 Msa1293640:CDS 45.0%
!!! AAGCCGAGGTTTTGGATCAG+TGG - chr8_3:51095971-51095990 None:intergenic 50.0%
ATCCAAAACCTCGGCTTCGA+TGG + chr8_3:51095974-51095993 Msa1293640:CDS 50.0%
GTTGATGCTGTTGCTCAGCT+TGG + chr8_3:51096021-51096040 Msa1293640:CDS 50.0%
CATGAGGGTTATGGGTGTGA+AGG + chr8_3:51096364-51096383 Msa1293640:CDS 50.0%
CCTGAAGATGGCTCTTAAGG+TGG - chr8_3:51096403-51096422 None:intergenic 50.0%
CCACCTTAAGAGCCATCTTC+AGG + chr8_3:51096400-51096419 Msa1293640:CDS 50.0%
ATAGAGAGGCCAACCCTTGA+TGG + chr8_3:51097958-51097977 Msa1293640:CDS 50.0%
!! GCTGCCACAGCATTGATCAT+AGG - chr8_3:51098270-51098289 None:intergenic 50.0%
ATGATCAATGCTGTGGCAGC+TGG + chr8_3:51098270-51098289 Msa1293640:CDS 50.0%
! GTCCATCGAAGCCGAGGTTT+TGG - chr8_3:51095979-51095998 None:intergenic 55.0%
GATGCTGTTGCTCAGCTTGG+AGG + chr8_3:51096024-51096043 Msa1293640:CDS 55.0%
GCTTGGAAAGCAACCCCACA+AGG + chr8_3:51096565-51096584 Msa1293640:CDS 55.0%
GGCACCTCGTCTTCTATGAC+TGG + chr8_3:51096762-51096781 Msa1293640:CDS 55.0%
GCGACCAGTCATAGAAGACG+AGG - chr8_3:51096769-51096788 None:intergenic 55.0%
TCGACGGTCCATCGAAGCCG+AGG - chr8_3:51095985-51096004 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 51095757 51098487 51095757 ID=Msa1293640;Name=Msa1293640
chr8_3 mRNA 51095757 51098487 51095757 ID=Msa1293640-mRNA-1;Parent=Msa1293640;Name=Msa1293640-mRNA-1;_AED=0.05;_eAED=0.05;_QI=117|0.8|0.83|1|1|1|6|170|343
chr8_3 exon 51095757 51096054 51095757 ID=Msa1293640-mRNA-1:exon:14238;Parent=Msa1293640-mRNA-1
chr8_3 exon 51096345 51096421 51096345 ID=Msa1293640-mRNA-1:exon:14239;Parent=Msa1293640-mRNA-1
chr8_3 exon 51096557 51096614 51096557 ID=Msa1293640-mRNA-1:exon:14240;Parent=Msa1293640-mRNA-1
chr8_3 exon 51096733 51096799 51096733 ID=Msa1293640-mRNA-1:exon:14241;Parent=Msa1293640-mRNA-1
chr8_3 exon 51097228 51097270 51097228 ID=Msa1293640-mRNA-1:exon:14242;Parent=Msa1293640-mRNA-1
chr8_3 exon 51097712 51098487 51097712 ID=Msa1293640-mRNA-1:exon:14243;Parent=Msa1293640-mRNA-1
chr8_3 five_prime_UTR 51095757 51095873 51095757 ID=Msa1293640-mRNA-1:five_prime_utr;Parent=Msa1293640-mRNA-1
chr8_3 CDS 51095874 51096054 51095874 ID=Msa1293640-mRNA-1:cds;Parent=Msa1293640-mRNA-1
chr8_3 CDS 51096345 51096421 51096345 ID=Msa1293640-mRNA-1:cds;Parent=Msa1293640-mRNA-1
chr8_3 CDS 51096557 51096614 51096557 ID=Msa1293640-mRNA-1:cds;Parent=Msa1293640-mRNA-1
chr8_3 CDS 51096733 51096799 51096733 ID=Msa1293640-mRNA-1:cds;Parent=Msa1293640-mRNA-1
chr8_3 CDS 51097228 51097270 51097228 ID=Msa1293640-mRNA-1:cds;Parent=Msa1293640-mRNA-1
chr8_3 CDS 51097712 51098317 51097712 ID=Msa1293640-mRNA-1:cds;Parent=Msa1293640-mRNA-1
chr8_3 three_prime_UTR 51098318 51098487 51098318 ID=Msa1293640-mRNA-1:three_prime_utr;Parent=Msa1293640-mRNA-1
Gene Sequence

>Msa1293640

ATGGAAAGAATGTTCCCTCCAAAGAAGCCCTCAACTATGATGAATTCTCATGATAGACCCATGTGTGTTCAAGGTGACTCTGGCCTTGTTCTCACCACTGATCCAAAACCTCGGCTTCGATGGACCGTCGAACTCCATGAACGCTTTGTTGATGCTGTTGCTCAGCTTGGAGGACCTGATAAAGCCACTCCAAAAACAATCATGAGGGTTATGGGTGTGAAGGGTCTTACCCTTTACCACCTTAAGAGCCATCTTCAGAAATTTAGGCTTGGAAAGCAACCCCACAAGGAATTCAATGAGCAATCTATTAAGGATGTTTCGGCTTTTGAACTGCAAAGAAATACTGGCACCTCGTCTTCTATGACTGGTCGCAACATGAATGAGATGCAAATGGAGGTGCACAGAAGACTGCATGAACAGCTAGAGGTTCAAAAGCACCTTCAATTGAGGATTGAGGCTCAAGGAAAATACATGCAAAGTATATTGGAGAAAGCTTATCACACACTTGCAGGTGAAAACATGGCAGCTGCAGCTACAAATTTCAAGGGTATGAATGGAACTCAAGGCATCCCTGACATGAAAGAATTTGTTTCTCCTCTAAATAATTTTCCTCATTTTCAAGATCTTAACATTTGTGGTAGTGATGATGAACAACTTGATCTTCATCACAACATAGAGAGGCCAACCCTTGATGGAATTATAATGCCTAATAATAATAATAATGAAGAAAATTTGTTTGTTGGAAAGAAAAGGACTAACCCTTTTAATGGAAGTGGAAAGAGTCCAATAATTTGGAATGATAATGATCTTAGGCTTCATGATTTGGGAACAACTTCATCATGCATTAGTCCTGATGATGTTCCTAATTTCAAAGGTGAATCATTGGATAGATGTGATGAAAGTGATTCTATTGTTGATATTTATGACACAAAAGTGAGTGAGAAGAAATTTGATCCATCTGTGAAGCTTGGAATAACATTACCTATGATCAATGCTGTGGCAGCTGGTAGAAGCTCATCACCATTTGGTTAA
Protein sequence

>Msa1293640

MERMFPPKKPSTMMNSHDRPMCVQGDSGLVLTTDPKPRLRWTVELHERFVDAVAQLGGPDKATPKTIMRVMGVKGLTLYHLKSHLQKFRLGKQPHKEFNEQSIKDVSAFELQRNTGTSSSMTGRNMNEMQMEVHRRLHEQLEVQKHLQLRIEAQGKYMQSILEKAYHTLAGENMAAAATNFKGMNGTQGIPDMKEFVSPLNNFPHFQDLNICGSDDEQLDLHHNIERPTLDGIIMPNNNNNEENLFVGKKRTNPFNGSGKSPIIWNDNDLRLHDLGTTSSCISPDDVPNFKGESLDRCDESDSIVDIYDTKVSEKKFDPSVKLGITLPMINAVAAGRSSSPFG*