AlfalfaGEDB | Msa1302590

M. sativa cultivar zhongmu-4 / Msa1302590

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1302590	sp\|Q2MHE4\|HT1_ARATH	82.821	390	53	3	8	383	1	390	0.0	652

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1302590	A0A396IIP0	99.479	384	1	1	1	383	1	384	0.0	795

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1223820	Msa1302590	0.808073	3.705326e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1302590	MtrunA17_Chr4g0075541	99.479	384	1	1	1	383	1	384	0.0	795
Msa1302590	MtrunA17_Chr3g0117731	61.635	318	115	2	56	373	30	340	2.69e-138	398
Msa1302590	MtrunA17_Chr4g0059121	58.255	321	131	1	56	376	31	348	3.68e-135	390
Msa1302590	MtrunA17_Chr4g0047191	50.993	302	143	3	71	367	29	330	3.09e-117	344
Msa1302590	MtrunA17_Chr4g0047211	50.993	302	143	3	71	367	24	325	4.18e-117	344
Msa1302590	MtrunA17_Chr4g0047201	49.405	336	160	4	21	354	143	470	7.44e-116	346
Msa1302590	MtrunA17_Chr2g0282321	49.271	343	168	4	17	354	90	431	1.18e-115	344
Msa1302590	MtrunA17_Chr3g0090261	51.119	268	131	0	74	341	70	337	7.26e-102	305
Msa1302590	MtrunA17_Chr8g0388891	45.149	268	126	1	74	341	121	367	1.66e-82	257
Msa1302590	MtrunA17_Chr1g0211611	42.424	264	147	2	79	342	294	552	1.46e-78	252

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1302590	AT1G62400.1	82.821	390	53	3	8	383	1	390	0.0	652
Msa1302590	AT5G58950.1	48.161	299	151	2	47	343	170	466	3.39e-103	315
Msa1302590	AT4G31170.7	51.698	265	128	0	77	341	122	386	1.33e-99	302
Msa1302590	AT4G31170.6	51.698	265	128	0	77	341	122	386	1.33e-99	302
Msa1302590	AT4G31170.5	51.698	265	128	0	77	341	122	386	1.33e-99	302
Msa1302590	AT4G31170.1	51.698	265	128	0	77	341	122	386	1.33e-99	302
Msa1302590	AT4G31170.2	51.698	265	128	0	77	341	122	386	1.33e-99	302
Msa1302590	AT4G31170.3	51.698	265	128	0	77	341	122	386	1.33e-99	302
Msa1302590	AT4G31170.8	51.698	265	128	0	77	341	122	386	1.33e-99	302
Msa1302590	AT2G24360.1	49.057	265	135	0	77	341	121	385	5.18e-91	280

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Find 117 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAGAACAGAAGCAAGGA+AGG	0.548560	8_3:-65174345	Msa1302590:five_prime_UTR
AAAGTTGATGTTTATAGTTT+TGG	0.278056	8_3:-65172274	Msa1302590:CDS
AAATTCATGCCTTTGGAATA+TGG	0.222756	8_3:+65173750	None:intergenic
AACAATAATTAATACCTTAT+AGG	0.328141	8_3:+65171371	None:intergenic
AACCGTGTCTTAGAAGATGA+AGG	0.574483	8_3:+65173642	None:intergenic
AATCAATCAATGTTAAGTAG+TGG	0.601441	8_3:+65174393	None:intergenic
AATTAATACCTTATAGGACA+GGG	0.616059	8_3:+65171377	None:intergenic
ACACGATGCCGAGAGACCAA+AGG	0.646712	8_3:-65172358	Msa1302590:CDS
ACAGCAAAAGCAGCTTGCAC+TGG	0.512530	8_3:+65172199	None:intergenic
ACATCAACTTTACGAGTATA+CGG	0.331341	8_3:+65172286	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TATTAGCCATAGAGAGAAAA+AGG	+	chr8_3:65171497-65171516	None:intergenic	30.0%
TTGAAAGGAAAAAGTTTCAG+GGG	+	chr8_3:65171558-65171577	None:intergenic	30.0%
CAGCATAAAGATGAATTGAA+AGG	+	chr8_3:65171573-65171592	None:intergenic	30.0%
TTGTAATAAGATGGATGAGA+AGG	+	chr8_3:65171611-65171630	None:intergenic	30.0%
TTCATGAAAATATGTGGAAG+AGG	+	chr8_3:65171672-65171691	None:intergenic	30.0%
GGAAGTTTCATGAAAATATG+TGG	+	chr8_3:65171678-65171697	None:intergenic	30.0%
AGACAGAAATGAACGTAAAA+GGG	+	chr8_3:65171744-65171763	None:intergenic	30.0%
ATGGTTCCATAATAACCAAA+AGG	-	chr8_3:65171838-65171857	Msa1302590:intron	30.0%
TGGTTCCATAATAACCAAAA+GGG	-	chr8_3:65171839-65171858	Msa1302590:intron	30.0%
CACAATAAACCATATTCCAA+AGG	-	chr8_3:65172031-65172050	Msa1302590:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	65171286	65174526	65171286	ID=Msa1302590;Name=Msa1302590
chr8_3	mRNA	65171286	65174526	65171286	ID=Msa1302590-mRNA-1;Parent=Msa1302590;Name=Msa1302590-mRNA-1;_AED=0.02;_eAED=0.02;_QI=769\|1\|1\|1\|1\|1\|3\|126\|383
chr8_3	exon	65171286	65171660	65171286	ID=Msa1302590-mRNA-1:exon:19750;Parent=Msa1302590-mRNA-1
chr8_3	exon	65172189	65172644	65172189	ID=Msa1302590-mRNA-1:exon:19749;Parent=Msa1302590-mRNA-1
chr8_3	exon	65173311	65174526	65173311	ID=Msa1302590-mRNA-1:exon:19748;Parent=Msa1302590-mRNA-1
chr8_3	five_prime_UTR	65173758	65174526	65173758	ID=Msa1302590-mRNA-1:five_prime_utr;Parent=Msa1302590-mRNA-1
chr8_3	CDS	65173311	65173757	65173311	ID=Msa1302590-mRNA-1:cds;Parent=Msa1302590-mRNA-1
chr8_3	CDS	65172189	65172644	65172189	ID=Msa1302590-mRNA-1:cds;Parent=Msa1302590-mRNA-1
chr8_3	CDS	65171412	65171660	65171412	ID=Msa1302590-mRNA-1:cds;Parent=Msa1302590-mRNA-1
chr8_3	three_prime_UTR	65171286	65171411	65171286	ID=Msa1302590-mRNA-1:three_prime_utr;Parent=Msa1302590-mRNA-1

Gene Sequence

>Msa1302590

ATGAATTTTGCTAGAGTTTCAATGGCAGGTTCATGTTTCACCAAACTTCGCCTGCGAAAGTCTAAGAGTAATAAACCTTTACCAGAGCCTTCTCCTTCATCTTCTAAGACACGGTTGAATCCTGATGCAGACAACATGGAAAGAAAAAGATTTGATAGTTTAGAATCATGGTCCATGATATTGGACTCTGAAAATGTCGAAACGTGGGAAGCTTCAAAGGAAGATCAGGAAGAATGGACTGCTGATCTTTCTCAGCTTTTCATAGGTAACAAGTTTGCGTCCGGTGCTCACAGTCGAATTTACCGTGGAATTTACAAGCAGAGAGCTGTTGCTGTTAAAATGGTGAGGATTCCTACTCAGAATGAGGAGAGAAGAACATTGCTAGAACAGCAATTCAAATCTGAAGTTGCTTTACTCTCACGTCTCTTTCATCCTAACATAGTGCAGTTTATTGCAGCATGTAAAAAACCACCTGTGTACTGTATAATAACAGAATACATGTCACAAGGAACTCTGAGGATGTATCTGAACAAAAAAGAGCCTTACTCACTTTCAATAGAAACTGTACTAAGGTTAGCACTTGACATATCAAGGGGTATGGAATACCTTCATTCTCAAGGTGTTATTCATAGAGACCTCAAATCAAATAACTTGCTTCTCAATGATGAGATGAGGGTAAAGGTAGCAGATTTCGGGACATCGTGTCTTGAAACACGATGCCGAGAGACCAAAGGAAATATGGGAACTTATCGTTGGATGGCACCTGAGATGATTAAGGAAAAACCGTATACTCGTAAAGTTGATGTTTATAGTTTTGGAATTGTGCTTTGGGAACTCACAACTGCTCTGCTTCCTTTCCAAGGAATGACTCCAGTGCAAGCTGCTTTTGCTGTTTCTGAGAAGAATGAAAGGCCTCCACTGCCTGCAAGTTGTCAGCCAGCACTTGCACACCTAATAAAGCGTTGTTGGTCAGCAAATCCTTCAAAGAGGCCAGATTTCAGTTACATTGTGTCTACTCTTGAGAGGTATGATGAATGTGTGAAGGAAGGTCTTCCTCTAACTCATCATTCAGGACTAGTCAGCAAAAACTTCATTATCGAACGCTTAAAAGGATGTGTCTCCATGAATTCCTCCATACCTGTAAATGCATAA

Protein sequence

>Msa1302590

MNFARVSMAGSCFTKLRLRKSKSNKPLPEPSPSSSKTRLNPDADNMERKRFDSLESWSMILDSENVETWEASKEDQEEWTADLSQLFIGNKFASGAHSRIYRGIYKQRAVAVKMVRIPTQNEERRTLLEQQFKSEVALLSRLFHPNIVQFIAACKKPPVYCIITEYMSQGTLRMYLNKKEPYSLSIETVLRLALDISRGMEYLHSQGVIHRDLKSNNLLLNDEMRVKVADFGTSCLETRCRETKGNMGTYRWMAPEMIKEKPYTRKVDVYSFGIVLWELTTALLPFQGMTPVQAAFAVSEKNERPPLPASCQPALAHLIKRCWSANPSKRPDFSYIVSTLERYDECVKEGLPLTHHSGLVSKNFIIERLKGCVSMNSSIPVNA*