AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1312430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1312430 MtrunA17_Chr8g0340641 95.263 380 17 1 1 380 1 379 0.0 748
Msa1312430 MtrunA17_Chr7g0242551 25.632 277 184 6 83 353 71 331 2.66e-15 76.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1312430 AT1G79760.1 35.135 333 136 13 11 339 1 257 5.67e-48 165
Msa1312430 AT1G79760.2 35.312 337 137 14 8 339 17 277 8.66e-48 165
Msa1312430 AT2G38500.1 27.333 300 181 12 29 324 28 294 4.28e-17 82.0
Msa1312430 AT5G05600.1 22.776 281 198 7 70 347 63 327 4.13e-12 67.4

Find 114 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAATTTCTCACCGAGTTTC+TGG 0.068021 8_4:-9407115 Msa1312430:CDS
ATGGCAACTACTATGTATTT+TGG 0.174246 8_4:+9403823 Msa1312420:three_prime_UTR
CATATTTGTTTGAAGTTTCT+TGG 0.181857 8_4:+9404366 None:intergenic
GAAGGTGGCTGAGAAAATTT+TGG 0.236901 8_4:-9405044 Msa1312430:CDS
AGTTGAAATTGGAGGGAATT+TGG 0.246627 8_4:-9406711 Msa1312430:CDS
TTTCGTCATTGATCTGTTTC+GGG 0.262623 8_4:+9407046 None:intergenic
ATTGGAAAGGCAATATTTAA+AGG 0.263472 8_4:-9404439 Msa1312430:CDS
TCATGCTTGCTCGGATGATT+TGG 0.283452 8_4:+9403733 Msa1312420:three_prime_UTR
CGGAAATCAACGGTGGTTTC+CGG 0.284712 8_4:+9407192 None:intergenic
AGTTGAATGAAGAATTTATT+TGG 0.285478 8_4:-9406756 Msa1312430:CDS
ATTACTAGGGCACCTTGTTC+AGG 0.285696 8_4:+9404758 None:intergenic
TAATGATCGCATTGTGAAAA+TGG 0.290296 8_4:+9407236 None:intergenic
GTTTCGTCATTGATCTGTTT+CGG 0.293381 8_4:+9407045 None:intergenic
TGAAGAGTTGAAGTTGAAAT+TGG 0.298562 8_4:-9406722 Msa1312430:CDS
AATTCGAAATACCTAAAATT+AGG 0.305210 8_4:+9406676 None:intergenic
GATCTCCAGCAGTGATTACT+AGG 0.312300 8_4:+9404744 None:intergenic
TTGCTGCACATACTGAGATT+TGG 0.313362 8_4:-9404002 Msa1312430:three_prime_UTR
CATATAATATTTATTTCATT+AGG 0.319188 8_4:+9403788 Msa1312420:three_prime_UTR
ACTGGAAAGTCTATAGTTAA+AGG 0.319842 8_4:-9403963 Msa1312430:three_prime_UTR
TGCTGCACATACTGAGATTT+GGG 0.320728 8_4:-9404001 Msa1312430:three_prime_UTR
CATTAGGTTCAAAGGTTTGA+TGG 0.330434 8_4:+9403804 Msa1312420:three_prime_UTR
CAAGAAACTTCAAACAAATA+TGG 0.338970 8_4:-9404365 Msa1312430:CDS
CAATACCAACTTCCTTGATT+AGG 0.349218 8_4:+9404027 Msa1312420:three_prime_UTR
AGCATCATCAAGATATTAAT+AGG 0.354575 8_4:-9404928 Msa1312430:CDS
ATGTGATTAGAATGCTGATT+AGG 0.357036 8_4:-9404874 Msa1312430:CDS
GCAAGTGATGGTGATATAAT+TGG 0.358719 8_4:-9404989 Msa1312430:CDS
GCTTGTTGGCCAAGTGAAAT+AGG 0.379497 8_4:+9404337 None:intergenic
TGTGATTAGAATGCTGATTA+GGG 0.384993 8_4:-9404873 Msa1312430:CDS
ACTTGGCCAACAAGCCATCT+TGG 0.392357 8_4:-9404329 Msa1312430:CDS
TTGAAATTGCAGCAAGAAGA+TGG 0.398906 8_4:-9405089 Msa1312430:intron
TTATCATATAATTGTTTAAG+TGG 0.400439 8_4:+9404176 None:intergenic
TTGTCAGGTAATACTAGATC+TGG 0.409379 8_4:+9407075 None:intergenic
TCTATAACTTACTACGTGGT+TGG 0.416323 8_4:+9404067 Msa1312420:three_prime_UTR
AGCGCAACGAAATCTACTTT+TGG 0.416834 8_4:+9407018 None:intergenic
ATCAACATTGTTCTCTGTAA+AGG 0.425593 8_4:-9407447 Msa1312430:five_prime_UTR
ATATTAATAGGAATGATCAT+TGG 0.434233 8_4:-9404916 Msa1312430:CDS
TGACTAGTTTATCCTAATCA+AGG 0.437981 8_4:-9404039 Msa1312430:three_prime_UTR
GTAGTCTTTGAGATTGTTGT+GGG 0.441896 8_4:+9404096 Msa1312420:three_prime_UTR
AGGAATATTTACGGTGCCGC+TGG 0.447791 8_4:-9406869 Msa1312430:CDS
GCTGCTGCAATGGAGGTTGC+CGG 0.454351 8_4:-9406892 Msa1312430:CDS
AGATCTCATCGACGTTGTTA+CGG 0.461660 8_4:-9406977 Msa1312430:CDS
TGATGAAGTAGAAGAAGAAG+AGG 0.462264 8_4:-9406788 Msa1312430:CDS
AAGAGAAGACAACATTTCAA+TGG 0.463032 8_4:-9404413 Msa1312430:CDS
GTTCAAAATTACAGCCAAGA+TGG 0.463235 8_4:+9404315 None:intergenic
GGTTGCCGGAGGAATATTTA+CGG 0.463294 8_4:-9406878 Msa1312430:CDS
TTACAGCCAAGATGGCTTGT+TGG 0.474725 8_4:+9404323 None:intergenic
TATTAATAGGAATGATCATT+GGG 0.475240 8_4:-9404915 Msa1312430:CDS
TGGTAAATTAAATGAGTACA+TGG 0.481095 8_4:+9403843 Msa1312420:three_prime_UTR
GGGCATTACAAGGATGTCAT+TGG 0.484026 8_4:-9404457 Msa1312430:CDS
GAGGTCGCCGGAGAAACCTC+GGG 0.489528 8_4:-9406827 Msa1312430:CDS
ATTGGTCATAGAAATGAAGT+TGG 0.490467 8_4:-9404971 Msa1312430:CDS
GGCTATAGGCATTGAGTGGA+GGG 0.491223 8_4:-9404477 Msa1312430:intron
ATGTTTACTCAAAGAAAAGT+TGG 0.492603 8_4:-9404793 Msa1312430:CDS
ATATTAGAACGTGGTACATA+AGG 0.493967 8_4:+9404293 None:intergenic
ATTTATTTCATTAGGTTCAA+AGG 0.496537 8_4:+9403796 Msa1312420:three_prime_UTR
TTACTAGGGCACCTTGTTCA+GGG 0.500069 8_4:+9404759 None:intergenic
CGACCGGAGGCTACCGGTGA+TGG 0.501289 8_4:+9407159 None:intergenic
GGGAGTTGTGGCTGCTGCAA+TGG 0.501427 8_4:-9406902 Msa1312430:CDS
TTATGTTTGCATGTGTGTGA+TGG 0.501762 8_4:-9404830 Msa1312430:CDS
TTTATCCTAATCAAGGAAGT+TGG 0.507700 8_4:-9404032 Msa1312430:three_prime_UTR
TTGTCTTTCTGCCCTGAACA+AGG 0.507702 8_4:-9404770 Msa1312430:CDS
ATTGCAGCAGCCACAACTCC+CGG 0.509540 8_4:+9406904 None:intergenic
TCAAAACCCCGAGGTTTCTC+CGG 0.510007 8_4:+9406820 None:intergenic
CTAAAATTAGGATATTCAAC+AGG 0.513293 8_4:+9406688 None:intergenic
CCGTGCACCGCCGCCATCAC+CGG 0.514670 8_4:-9407172 Msa1312430:CDS
CCGGTGATGGCGGCGGTGCA+CGG 0.517525 8_4:+9407172 None:intergenic
TGTTTCGGGAAAATCTTGTC+AGG 0.520100 8_4:+9407060 None:intergenic
ACGGAATCAATAGCTAGGTT+CGG 0.520536 8_4:-9406958 Msa1312430:CDS
TGTAGTCTTTGAGATTGTTG+TGG 0.521495 8_4:+9404095 Msa1312420:three_prime_UTR
ATCAACGGTGGTTTCCGGCA+TGG 0.532122 8_4:+9407197 None:intergenic
GGGAACTATAAGCAAGTGAC+TGG 0.533650 8_4:-9403981 Msa1312430:three_prime_UTR
ATTAACGCCGCGAACCATGC+CGG 0.535370 8_4:-9407211 Msa1312430:CDS
TTGGTCATAGAAATGAAGTT+GGG 0.535395 8_4:-9404970 Msa1312430:CDS
GAGTTGAAGTTGAAATTGGA+GGG 0.535578 8_4:-9406718 Msa1312430:CDS
CGGGATATCACCGGGAGTTG+TGG 0.536090 8_4:-9406914 Msa1312430:CDS
GGTGCCCTAGTAATCACTGC+TGG 0.539493 8_4:-9404749 Msa1312430:CDS
AGAGTTGAAGTTGAAATTGG+AGG 0.544255 8_4:-9406719 Msa1312430:CDS
CACTGCTGGAGATCAAATCC+AGG 0.545215 8_4:-9404735 Msa1312430:intron
CGAGGTCGCCGGAGAAACCT+CGG 0.545941 8_4:-9406828 Msa1312430:CDS
GAGGCTACCGGTGATGGCGG+CGG 0.552842 8_4:+9407165 None:intergenic
TATCAACCACGGGATATCAC+CGG 0.553591 8_4:-9406923 Msa1312430:CDS
GCTTTCAGCTTATCAACCAC+GGG 0.555684 8_4:-9406933 Msa1312430:CDS
TGGCTATAGGCATTGAGTGG+AGG 0.560050 8_4:-9404478 Msa1312430:intron
TGCTTTCAGCTTATCAACCA+CGG 0.562183 8_4:-9406934 Msa1312430:CDS
TCTACAATCACGTGTGGAGA+AGG 0.568223 8_4:-9405062 Msa1312430:CDS
CGGCACCGTAAATATTCCTC+CGG 0.568409 8_4:+9406873 None:intergenic
ATGGAAAGACCTATTTCACT+TGG 0.570120 8_4:-9404346 Msa1312430:CDS
GATTCTGTTCACATTTCAAG+AGG 0.573329 8_4:-9403712 Msa1312430:three_prime_UTR
TGAACAGAATCATGCTTGCT+CGG 0.578747 8_4:+9403724 Msa1312420:three_prime_UTR
AGATGCTGTGACGAGGTCGC+CGG 0.580442 8_4:-9406839 Msa1312430:CDS
ATTGAGTGGAGGGCATTACA+AGG 0.581852 8_4:-9404467 Msa1312430:CDS
TTGTTACGGAATCAATAGCT+AGG 0.582781 8_4:-9406963 Msa1312430:CDS
ATCAACCACGGGATATCACC+GGG 0.596614 8_4:-9406922 Msa1312430:CDS
CGGCGGTGCACGGAAATCAA+CGG 0.614742 8_4:+9407182 None:intergenic
AGTTGTGGCTGCTGCAATGG+AGG 0.618808 8_4:-9406899 Msa1312430:CDS
ATGATAGTGTGTCTGTTGAT+CGG 0.620069 8_4:+9407360 None:intergenic
TGTTACCGTTGATCTACGAC+CGG 0.620887 8_4:+9407143 None:intergenic
ATCTCCAGCAGTGATTACTA+GGG 0.624399 8_4:+9404745 None:intergenic
TTACAAGGATGTCATTGGAA+AGG 0.624474 8_4:-9404452 Msa1312430:CDS
ACAATCACGTGTGGAGAAGG+TGG 0.625775 8_4:-9405059 Msa1312430:CDS
CAACTCCCGGTGATATCCCG+TGG 0.626338 8_4:+9406917 None:intergenic
GCTGCAATGGAGGTTGCCGG+AGG 0.635644 8_4:-9406889 Msa1312430:CDS
GCTTGGCTATAGGCATTGAG+TGG 0.637199 8_4:-9404481 Msa1312430:intron
AAGCGAAGTTTCCAGAAACT+CGG 0.638861 8_4:+9407104 None:intergenic
AGCCTCCGGTCGTAGATCAA+CGG 0.648849 8_4:-9407148 Msa1312430:CDS
GGAAACTCTACAATCACGTG+TGG 0.665148 8_4:-9405068 Msa1312430:CDS
ACAGCATCTCTCATTTCCAG+CGG 0.692882 8_4:+9406853 None:intergenic
TAAAGACAAATATTAGAACG+TGG 0.700039 8_4:+9404284 None:intergenic
TGATGCTCTTCAAAACCCCG+AGG 0.718916 8_4:+9406811 None:intergenic
AAATGAGAGATGCTGTGACG+AGG 0.726344 8_4:-9406846 Msa1312430:CDS
AGGTCGCCGGAGAAACCTCG+GGG 0.736808 8_4:-9406826 Msa1312430:CDS
CGGTGCACGGAAATCAACGG+TGG 0.737046 8_4:+9407185 None:intergenic
GTGATTAGAATGCTGATTAG+GGG 0.740101 8_4:-9404872 Msa1312430:CDS
TTTATCTATAACTTACTACG+TGG 0.746580 8_4:+9404063 Msa1312420:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTAGATTTTTTTTTTGAA+AGG - chr8_4:9404984-9405003 Msa1312430:CDS 10.0%
!! CATATAATATTTATTTCATT+AGG + chr8_4:9407361-9407380 None:intergenic 10.0%
!!! TACGATATATTTTAACAATT+CGG + chr8_4:9404819-9404838 None:intergenic 15.0%
!!! TGAAAGAATATGTATTTTAT+AGG - chr8_4:9405455-9405474 Msa1312430:intron 15.0%
!!! ATGTTTTAAAGTAGAAAAAT+TGG - chr8_4:9405659-9405678 Msa1312430:intron 15.0%
!!! GTTTATTTTGTATATATTGA+TGG - chr8_4:9405976-9405995 Msa1312430:intron 15.0%
!! TTATCATATAATTGTTTAAG+TGG + chr8_4:9406973-9406992 None:intergenic 15.0%
!! AGTTGAATGAAGAATTTATT+TGG - chr8_4:9404390-9404409 Msa1312430:CDS 20.0%
!! AATTCGAAATACCTAAAATT+AGG + chr8_4:9404473-9404492 None:intergenic 20.0%
!!! AATCATAAGTAGTTTGTATT+AGG + chr8_4:9404918-9404937 None:intergenic 20.0%
!!! TATATAATATTGGGTTTTGA+CGG - chr8_4:9405072-9405091 Msa1312430:CDS 20.0%
!!! ATTTTTTTCTTCGTTTTTTG+AGG + chr8_4:9405930-9405949 None:intergenic 20.0%
!! AAAACGAAGAAAAAAATAGT+AGG - chr8_4:9405933-9405952 Msa1312430:intron 20.0%
!! AAGAAAAAAATAGTAGGTAT+TGG - chr8_4:9405939-9405958 Msa1312430:intron 20.0%
!! ATATTAATAGGAATGATCAT+TGG - chr8_4:9406230-9406249 Msa1312430:intron 20.0%
!! TATTAATAGGAATGATCATT+GGG - chr8_4:9406231-9406250 Msa1312430:intron 20.0%
!!! AGATTATGCATAAGATTTTA+AGG + chr8_4:9407022-9407041 None:intergenic 20.0%
!! ATTTATTTCATTAGGTTCAA+AGG + chr8_4:9407353-9407372 None:intergenic 20.0%
! CTAAAATTAGGATATTCAAC+AGG + chr8_4:9404461-9404480 None:intergenic 25.0%
!! CTGTTGAATATCCTAATTTT+AGG - chr8_4:9404459-9404478 Msa1312430:CDS 25.0%
! TTGAGCTTCAAAGTTTATTA+AGG + chr8_4:9404705-9404724 None:intergenic 25.0%
!! TTTAGATGAGTTGAATTTCA+AGG + chr8_4:9404777-9404796 None:intergenic 25.0%
!!! ATATAATATTGGGTTTTGAC+GGG - chr8_4:9405073-9405092 Msa1312430:CDS 25.0%
! CTCAAAATCTTTATTATGCA+AGG + chr8_4:9405617-9405636 None:intergenic 25.0%
!! CTTGCATAATAAAGATTTTG+AGG - chr8_4:9405615-9405634 Msa1312430:intron 25.0%
!! AACAATCTATCTTTACGTTT+TGG - chr8_4:9405787-9405806 Msa1312430:intron 25.0%
! ATGTACGTATCTAAATTTGT+CGG + chr8_4:9405853-9405872 None:intergenic 25.0%
! AAAAAGAAATTAGCAAGTGA+TGG - chr8_4:9406145-9406164 Msa1312430:intron 25.0%
! AGCATCATCAAGATATTAAT+AGG - chr8_4:9406218-9406237 Msa1312430:intron 25.0%
! ATGTTTACTCAAAGAAAAGT+TGG - chr8_4:9406353-9406372 Msa1312430:intron 25.0%
! AGCAAAAATGTAAAATTACC+TGG + chr8_4:9406432-9406451 None:intergenic 25.0%
!! TGAAGGTATAGTCAAAATAT+TGG + chr8_4:9406579-9406598 None:intergenic 25.0%
! ATTGGAAAGGCAATATTTAA+AGG - chr8_4:9406707-9406726 Msa1312430:CDS 25.0%
!! CATATTTGTTTGAAGTTTCT+TGG + chr8_4:9406783-9406802 None:intergenic 25.0%
! CAAGAAACTTCAAACAAATA+TGG - chr8_4:9406781-9406800 Msa1312430:CDS 25.0%
! TAAAGACAAATATTAGAACG+TGG + chr8_4:9406865-9406884 None:intergenic 25.0%
! TTTATCTATAACTTACTACG+TGG + chr8_4:9407086-9407105 None:intergenic 25.0%
! TGGTAAATTAAATGAGTACA+TGG + chr8_4:9407306-9407325 None:intergenic 25.0%
! ATTTGGACTTCATTAAAAAG+TGG + chr8_4:9407399-9407418 None:intergenic 25.0%
TAATGATCGCATTGTGAAAA+TGG + chr8_4:9403913-9403932 Msa1312420:three_prime_UTR 30.0%
TGAAGAGTTGAAGTTGAAAT+TGG - chr8_4:9404424-9404443 Msa1312430:CDS 30.0%
TGATTCTCGTAAATCAAACT+CGG + chr8_4:9404646-9404665 None:intergenic 30.0%
TACAATTACACACACTCATT+TGG - chr8_4:9405177-9405196 Msa1312430:intron 30.0%
ACAATTACACACACTCATTT+GGG - chr8_4:9405178-9405197 Msa1312430:intron 30.0%
! AGTTTTTATCAAGATTGACG+TGG - chr8_4:9405330-9405349 Msa1312430:intron 30.0%
! GTTTTTATCAAGATTGACGT+GGG - chr8_4:9405331-9405350 Msa1312430:intron 30.0%
! TAAGAGTGTGAGATATAAAG+TGG - chr8_4:9405396-9405415 Msa1312430:intron 30.0%
AATTATGTACCTTGCATTTG+TGG - chr8_4:9405432-9405451 Msa1312430:intron 30.0%
!!! GTATTTTATAGGCACTGATA+AGG - chr8_4:9405466-9405485 Msa1312430:intron 30.0%
TGTACGTATCTAAATTTGTC+GGG + chr8_4:9405852-9405871 None:intergenic 30.0%
ATTGGTCATAGAAATGAAGT+TGG - chr8_4:9406175-9406194 Msa1312430:intron 30.0%
TTGGTCATAGAAATGAAGTT+GGG - chr8_4:9406176-9406195 Msa1312430:intron 30.0%
! ATGTGATTAGAATGCTGATT+AGG - chr8_4:9406272-9406291 Msa1312430:intron 30.0%
! TGTGATTAGAATGCTGATTA+GGG - chr8_4:9406273-9406292 Msa1312430:intron 30.0%
TTAACGAGTTTGATATTGCT+TGG - chr8_4:9406648-9406667 Msa1312430:intron 30.0%
AAGAGAAGACAACATTTCAA+TGG - chr8_4:9406733-9406752 Msa1312430:CDS 30.0%
ATATTAGAACGTGGTACATA+AGG + chr8_4:9406856-9406875 None:intergenic 30.0%
!!! TAAGATTTTAAGGTGCAAAC+AGG + chr8_4:9407012-9407031 None:intergenic 30.0%
!! TGACTAGTTTATCCTAATCA+AGG - chr8_4:9407107-9407126 Msa1312430:CDS 30.0%
TTTATCCTAATCAAGGAAGT+TGG - chr8_4:9407114-9407133 Msa1312430:CDS 30.0%
ACTGGAAAGTCTATAGTTAA+AGG - chr8_4:9407183-9407202 Msa1312430:CDS 30.0%
! ATGGCAACTACTATGTATTT+TGG + chr8_4:9407326-9407345 None:intergenic 30.0%
TTCATTAAAAAGTGGACAGT+GGG + chr8_4:9407391-9407410 None:intergenic 30.0%
! ATGATAGTGTGTCTGTTGAT+CGG + chr8_4:9403789-9403808 Msa1312420:three_prime_UTR 35.0%
TTGTCAGGTAATACTAGATC+TGG + chr8_4:9404074-9404093 Msa1312420:three_prime_UTR 35.0%
TTTCGTCATTGATCTGTTTC+GGG + chr8_4:9404103-9404122 Msa1312420:three_prime_UTR 35.0%
GTTTCGTCATTGATCTGTTT+CGG + chr8_4:9404104-9404123 Msa1312420:three_prime_UTR 35.0%
TTGTTACGGAATCAATAGCT+AGG - chr8_4:9404183-9404202 Msa1312430:three_prime_UTR 35.0%
TGATGAAGTAGAAGAAGAAG+AGG - chr8_4:9404358-9404377 Msa1312430:CDS 35.0%
AGAGTTGAAGTTGAAATTGG+AGG - chr8_4:9404427-9404446 Msa1312430:CDS 35.0%
GAGTTGAAGTTGAAATTGGA+GGG - chr8_4:9404428-9404447 Msa1312430:CDS 35.0%
AGTTGAAATTGGAGGGAATT+TGG - chr8_4:9404435-9404454 Msa1312430:CDS 35.0%
TTAACTTACGTCCAAAGTTG+TGG - chr8_4:9404611-9404630 Msa1312430:intron 35.0%
TGAATTTCAAGGTTTCACCA+AGG + chr8_4:9404766-9404785 None:intergenic 35.0%
CTTACGTGATCAAGAAGAAT+TGG - chr8_4:9405295-9405314 Msa1312430:intron 35.0%
ATTCTTTCACCACAAATGCA+AGG + chr8_4:9405444-9405463 None:intergenic 35.0%
CTAAAACACAAGCTCAATCA+TGG + chr8_4:9405594-9405613 None:intergenic 35.0%
TTGAAATTGCAGCAAGAAGA+TGG - chr8_4:9406057-9406076 Msa1312430:intron 35.0%
! GCAAGTGATGGTGATATAAT+TGG - chr8_4:9406157-9406176 Msa1312430:intron 35.0%
! GTGATTAGAATGCTGATTAG+GGG - chr8_4:9406274-9406293 Msa1312430:intron 35.0%
TTATGTTTGCATGTGTGTGA+TGG - chr8_4:9406316-9406335 Msa1312430:intron 35.0%
AAAAAACTCCAACTCCACTT+TGG - chr8_4:9406491-9406510 Msa1312430:intron 35.0%
ATTTCAAACCAAAGTGGAGT+TGG + chr8_4:9406502-9406521 None:intergenic 35.0%
GTTCGTATTTCAAACCAAAG+TGG + chr8_4:9406508-9406527 None:intergenic 35.0%
!! TTTTGCTGTCATGAGATTGA+AGG + chr8_4:9406596-9406615 None:intergenic 35.0%
TCAAACTCGTTAAGTGAGAT+GGG + chr8_4:9406641-9406660 None:intergenic 35.0%
ATCAAACTCGTTAAGTGAGA+TGG + chr8_4:9406642-9406661 None:intergenic 35.0%
GTTTGATATTGCTTGGCTAT+AGG - chr8_4:9406655-9406674 Msa1312430:intron 35.0%
TTACAAGGATGTCATTGGAA+AGG - chr8_4:9406694-9406713 Msa1312430:CDS 35.0%
! TGAAGTTTCTTGGCATTTTG+AGG + chr8_4:9406773-9406792 None:intergenic 35.0%
ATGGAAAGACCTATTTCACT+TGG - chr8_4:9406800-9406819 Msa1312430:CDS 35.0%
GTTCAAAATTACAGCCAAGA+TGG + chr8_4:9406834-9406853 None:intergenic 35.0%
!! GTAGTCTTTGAGATTGTTGT+GGG + chr8_4:9407053-9407072 None:intergenic 35.0%
!! TGTAGTCTTTGAGATTGTTG+TGG + chr8_4:9407054-9407073 None:intergenic 35.0%
TCTATAACTTACTACGTGGT+TGG + chr8_4:9407082-9407101 None:intergenic 35.0%
CAATACCAACTTCCTTGATT+AGG + chr8_4:9407122-9407141 None:intergenic 35.0%
CATTAGGTTCAAAGGTTTGA+TGG + chr8_4:9407345-9407364 None:intergenic 35.0%
CTTCATTAAAAAGTGGACAG+TGG + chr8_4:9407392-9407411 None:intergenic 35.0%
GATTCTGTTCACATTTCAAG+AGG - chr8_4:9407434-9407453 Msa1312430:five_prime_UTR 35.0%
AAGCGAAGTTTCCAGAAACT+CGG + chr8_4:9404045-9404064 Msa1312420:three_prime_UTR 40.0%
TGTTTCGGGAAAATCTTGTC+AGG + chr8_4:9404089-9404108 Msa1312420:three_prime_UTR 40.0%
!! GCAACGAAATCTACTTTTGG+CGG + chr8_4:9404128-9404147 None:intergenic 40.0%
! AGCGCAACGAAATCTACTTT+TGG + chr8_4:9404131-9404150 None:intergenic 40.0%
AGATCTCATCGACGTTGTTA+CGG - chr8_4:9404169-9404188 Msa1312430:three_prime_UTR 40.0%
ACGGAATCAATAGCTAGGTT+CGG - chr8_4:9404188-9404207 Msa1312430:three_prime_UTR 40.0%
TGCTTTCAGCTTATCAACCA+CGG - chr8_4:9404212-9404231 Msa1312430:three_prime_UTR 40.0%
GGAAGTTGAATCCACAACTT+TGG + chr8_4:9404625-9404644 None:intergenic 40.0%
ACACACACTCATTTGGGAAA+TGG - chr8_4:9405184-9405203 Msa1312430:intron 40.0%
! GAAGGTGGCTGAGAAAATTT+TGG - chr8_4:9406102-9406121 Msa1312430:intron 40.0%
ATCTCCAGCAGTGATTACTA+GGG + chr8_4:9406404-9406423 None:intergenic 40.0%
ATTGGCACACTAGCATTAAG+AGG + chr8_4:9406561-9406580 None:intergenic 40.0%
!! TGGCATTTTGAGGAGAATAG+AGG + chr8_4:9406763-9406782 None:intergenic 40.0%
TTGCTGCACATACTGAGATT+TGG - chr8_4:9407144-9407163 Msa1312430:CDS 40.0%
TGCTGCACATACTGAGATTT+GGG - chr8_4:9407145-9407164 Msa1312430:CDS 40.0%
TGAACAGAATCATGCTTGCT+CGG + chr8_4:9407425-9407444 None:intergenic 40.0%
TGTTACCGTTGATCTACGAC+CGG + chr8_4:9404006-9404025 Msa1312420:three_prime_UTR 45.0%
CGAATTTCTCACCGAGTTTC+TGG - chr8_4:9404031-9404050 Msa1312430:three_prime_UTR 45.0%
GCTTTCAGCTTATCAACCAC+GGG - chr8_4:9404213-9404232 Msa1312430:three_prime_UTR 45.0%
TATCAACCACGGGATATCAC+CGG - chr8_4:9404223-9404242 Msa1312430:three_prime_UTR 45.0%
GGTTGCCGGAGGAATATTTA+CGG - chr8_4:9404268-9404287 Msa1312430:exon 45.0%
ACAGCATCTCTCATTTCCAG+CGG + chr8_4:9404296-9404315 None:intergenic 45.0%
AAATGAGAGATGCTGTGACG+AGG - chr8_4:9404300-9404319 Msa1312430:CDS 45.0%
!!! TTTTGAGACAGTCCTGACCT+TGG - chr8_4:9404746-9404765 Msa1312430:CDS 45.0%
TTCAAGGTTTCACCAAGGTC+AGG + chr8_4:9404761-9404780 None:intergenic 45.0%
!! TTGGGTTTTGACGGGAAACA+AGG - chr8_4:9405081-9405100 Msa1312430:intron 45.0%
!! TGGGTTTTGACGGGAAACAA+GGG - chr8_4:9405082-9405101 Msa1312430:intron 45.0%
GATAAGGAGTCGTTAGAGAG+CGG - chr8_4:9405482-9405501 Msa1312430:intron 45.0%
AGTCGTTAGAGAGCGGTAAA+AGG - chr8_4:9405489-9405508 Msa1312430:intron 45.0%
CAATGGCTAGCAATAGTCGT+TGG + chr8_4:9405548-9405567 None:intergenic 45.0%
ACGACTATTGCTAGCCATTG+AGG - chr8_4:9405548-9405567 Msa1312430:intron 45.0%
ACTATCCTGTAACGCCTCAA+TGG + chr8_4:9405565-9405584 None:intergenic 45.0%
GGAAACTCTACAATCACGTG+TGG - chr8_4:9406078-9406097 Msa1312430:intron 45.0%
TCTACAATCACGTGTGGAGA+AGG - chr8_4:9406084-9406103 Msa1312430:intron 45.0%
TTGTCTTTCTGCCCTGAACA+AGG - chr8_4:9406376-9406395 Msa1312430:intron 45.0%
! TTACTAGGGCACCTTGTTCA+GGG + chr8_4:9406390-9406409 None:intergenic 45.0%
! ATTACTAGGGCACCTTGTTC+AGG + chr8_4:9406391-9406410 None:intergenic 45.0%
GATCTCCAGCAGTGATTACT+AGG + chr8_4:9406405-9406424 None:intergenic 45.0%
!! ATTGAGTGGAGGGCATTACA+AGG - chr8_4:9406679-9406698 Msa1312430:intron 45.0%
GGGCATTACAAGGATGTCAT+TGG - chr8_4:9406689-9406708 Msa1312430:CDS 45.0%
! GCTTGTTGGCCAAGTGAAAT+AGG + chr8_4:9406812-9406831 None:intergenic 45.0%
! TTACAGCCAAGATGGCTTGT+TGG + chr8_4:9406826-9406845 None:intergenic 45.0%
GGGAACTATAAGCAAGTGAC+TGG - chr8_4:9407165-9407184 Msa1312430:CDS 45.0%
TCATGCTTGCTCGGATGATT+TGG + chr8_4:9407416-9407435 None:intergenic 45.0%
!!! TAAAAAAGTATATATAATAT+TGG - chr8_4:9405062-9405081 Msa1312430:CDS 5.0%
!!! AAAAAAGTATATATAATATT+GGG - chr8_4:9405063-9405082 Msa1312430:CDS 5.0%
CGGAAATCAACGGTGGTTTC+CGG + chr8_4:9403957-9403976 Msa1312420:three_prime_UTR 50.0%
ATCAACCACGGGATATCACC+GGG - chr8_4:9404224-9404243 Msa1312430:three_prime_UTR 50.0%
CGGCACCGTAAATATTCCTC+CGG + chr8_4:9404276-9404295 None:intergenic 50.0%
!! AGGAATATTTACGGTGCCGC+TGG - chr8_4:9404277-9404296 Msa1312430:CDS 50.0%
TCAAAACCCCGAGGTTTCTC+CGG + chr8_4:9404329-9404348 None:intergenic 50.0%
TGATGCTCTTCAAAACCCCG+AGG + chr8_4:9404338-9404357 None:intergenic 50.0%
!! CATTGACTTGAGCGTCAGAG+TGG + chr8_4:9405370-9405389 None:intergenic 50.0%
TAGATTCCGTGCAACTGGAG+GGG + chr8_4:9405736-9405755 None:intergenic 50.0%
CTAGATTCCGTGCAACTGGA+GGG + chr8_4:9405737-9405756 None:intergenic 50.0%
GAACCTAGATTCCGTGCAAC+TGG + chr8_4:9405741-9405760 None:intergenic 50.0%
ACAATCACGTGTGGAGAAGG+TGG - chr8_4:9406087-9406106 Msa1312430:intron 50.0%
CACTGCTGGAGATCAAATCC+AGG - chr8_4:9406411-9406430 Msa1312430:intron 50.0%
!! GCTTGGCTATAGGCATTGAG+TGG - chr8_4:9406665-9406684 Msa1312430:intron 50.0%
!! TGGCTATAGGCATTGAGTGG+AGG - chr8_4:9406668-9406687 Msa1312430:intron 50.0%
!! GGCTATAGGCATTGAGTGGA+GGG - chr8_4:9406669-9406688 Msa1312430:intron 50.0%
ACTTGGCCAACAAGCCATCT+TGG - chr8_4:9406817-9406836 Msa1312430:CDS 50.0%
ATTAACGCCGCGAACCATGC+CGG - chr8_4:9403935-9403954 Msa1312430:three_prime_UTR 55.0%
ATCAACGGTGGTTTCCGGCA+TGG + chr8_4:9403952-9403971 Msa1312420:three_prime_UTR 55.0%
AGCCTCCGGTCGTAGATCAA+CGG - chr8_4:9403998-9404017 Msa1312430:three_prime_UTR 55.0%
TACCGTTGATCTACGACCGG+AGG + chr8_4:9404003-9404022 Msa1312420:three_prime_UTR 55.0%
ATTGCAGCAGCCACAACTCC+CGG + chr8_4:9404245-9404264 None:intergenic 55.0%
AGTTGTGGCTGCTGCAATGG+AGG - chr8_4:9404247-9404266 Msa1312430:three_prime_UTR 55.0%
TTGAGCGTCAGAGTGGCTAC+AGG + chr8_4:9405363-9405382 None:intergenic 55.0%
! GCTAGCCATTGAGGCGTTAC+AGG - chr8_4:9405557-9405576 Msa1312430:intron 55.0%
AGATTCCCCCTCCAGTTGCA+CGG - chr8_4:9405727-9405746 Msa1312430:intron 55.0%
AGATTCCGTGCAACTGGAGG+GGG + chr8_4:9405735-9405754 None:intergenic 55.0%
CCTAGATTCCGTGCAACTGG+AGG + chr8_4:9405738-9405757 None:intergenic 55.0%
CCTCCAGTTGCACGGAATCT+AGG - chr8_4:9405735-9405754 Msa1312430:intron 55.0%
GGTGCCCTAGTAATCACTGC+TGG - chr8_4:9406397-9406416 Msa1312430:intron 55.0%
GTGGTTTCCGGCATGGTTCG+CGG + chr8_4:9403945-9403964 Msa1312420:three_prime_UTR 60.0%
CGGTGCACGGAAATCAACGG+TGG + chr8_4:9403964-9403983 Msa1312420:three_prime_UTR 60.0%
CGGCGGTGCACGGAAATCAA+CGG + chr8_4:9403967-9403986 Msa1312420:three_prime_UTR 60.0%
GATCTACGACCGGAGGCTAC+CGG + chr8_4:9403996-9404015 Msa1312420:three_prime_UTR 60.0%
CAACTCCCGGTGATATCCCG+TGG + chr8_4:9404232-9404251 None:intergenic 60.0%
CGGGATATCACCGGGAGTTG+TGG - chr8_4:9404232-9404251 Msa1312430:three_prime_UTR 60.0%
GGGAGTTGTGGCTGCTGCAA+TGG - chr8_4:9404244-9404263 Msa1312430:three_prime_UTR 60.0%
GCTGCTGCAATGGAGGTTGC+CGG - chr8_4:9404254-9404273 Msa1312430:exon 60.0%
AGATGCTGTGACGAGGTCGC+CGG - chr8_4:9404307-9404326 Msa1312430:CDS 60.0%
GCTGCAATGGAGGTTGCCGG+AGG - chr8_4:9404257-9404276 Msa1312430:exon 65.0%
CGAGGTCGCCGGAGAAACCT+CGG - chr8_4:9404318-9404337 Msa1312430:CDS 65.0%
GAGGTCGCCGGAGAAACCTC+GGG - chr8_4:9404319-9404338 Msa1312430:CDS 65.0%
AGGTCGCCGGAGAAACCTCG+GGG - chr8_4:9404320-9404339 Msa1312430:CDS 65.0%
!! GAGGCTACCGGTGATGGCGG+CGG + chr8_4:9403984-9404003 Msa1312420:three_prime_UTR 70.0%
! CCGGAGGCTACCGGTGATGG+CGG + chr8_4:9403987-9404006 Msa1312420:three_prime_UTR 70.0%
CCGCCATCACCGGTAGCCTC+CGG - chr8_4:9403984-9404003 Msa1312430:three_prime_UTR 70.0%
CGACCGGAGGCTACCGGTGA+TGG + chr8_4:9403990-9404009 Msa1312420:three_prime_UTR 70.0%
!! CCGGTGATGGCGGCGGTGCA+CGG + chr8_4:9403977-9403996 Msa1312420:three_prime_UTR 75.0%
CCGTGCACCGCCGCCATCAC+CGG - chr8_4:9403974-9403993 Msa1312430:three_prime_UTR 75.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 9403701 9407467 9403701 ID=Msa1312430;Name=Msa1312430
chr8_4 mRNA 9403701 9407467 9403701 ID=Msa1312430-mRNA-1;Parent=Msa1312430;Name=Msa1312430-mRNA-1;_AED=0.14;_eAED=0.14;_QI=220|1|1|1|1|1|3|572|380
chr8_4 exon 9403701 9404491 9403701 ID=Msa1312430-mRNA-1:exon:3501;Parent=Msa1312430-mRNA-1
chr8_4 exon 9404736 9405099 9404736 ID=Msa1312430-mRNA-1:exon:3500;Parent=Msa1312430-mRNA-1
chr8_4 exon 9406688 9407467 9406688 ID=Msa1312430-mRNA-1:exon:3499;Parent=Msa1312430-mRNA-1
chr8_4 five_prime_UTR 9407248 9407467 9407248 ID=Msa1312430-mRNA-1:five_prime_utr;Parent=Msa1312430-mRNA-1
chr8_4 CDS 9406688 9407247 9406688 ID=Msa1312430-mRNA-1:cds;Parent=Msa1312430-mRNA-1
chr8_4 CDS 9404736 9405099 9404736 ID=Msa1312430-mRNA-1:cds;Parent=Msa1312430-mRNA-1
chr8_4 CDS 9404273 9404491 9404273 ID=Msa1312430-mRNA-1:cds;Parent=Msa1312430-mRNA-1
chr8_4 three_prime_UTR 9403701 9404272 9403701 ID=Msa1312430-mRNA-1:three_prime_utr;Parent=Msa1312430-mRNA-1
Gene Sequence

>Msa1312430

ATGCGATCATTAATATTAACGCCGCGAACCATGCCGGAAACCACCGTTGATTTCCGTGCACCGCCGCCATCACCGGTAGCCTCCGGTCGTAGATCAACGGTAACAAACAACGAATTTCTCACCGAGTTTCTGGAAACTTCGCTTCGAGTTCCAGATCTAGTATTACCTGACAAGATTTTCCCGAAACAGATCAATGACGAAACGCCGCCAAAAGTAGATTTCGTTGCGCTATGTTTTCACGAAGATGAAGATCTCATCGACGTTGTTACGGAATCAATAGCTAGGTTCGGATGCTTTCAGCTTATCAACCACGGGATATCACCGGGAGTTGTGGCTGCTGCAATGGAGGTTGCCGGAGGAATATTTACGGTGCCGCTGGAAATGAGAGATGCTGTGACGAGGTCGCCGGAGAAACCTCGGGGTTTTGAAGAGCATCATGATGAAGTAGAAGAAGAAGAGGAGAGAAGAGAGTTGAATGAAGAATTTATTTGGTGTAAGAATGATGAAGAGTTGAAGTTGAAATTGGAGGGAATTTGGCCTGTTGAATATCCTAATTTTAGCAAGAAGATGGAAACTCTACAATCACGTGTGGAGAAGGTGGCTGAGAAAATTTTGGATATCATATTGAAAAATGTTAAAAAGAAATTAGCAAGTGATGGTGATATAATTGGTCATAGAAATGAAGTTGGGACACTTTGTTGCATTTACAAGCATCATCAAGATATTAATAGGAATGATCATTGGGTTGATTCTTTGAAATATGATGTGATTAGAATGCTGATTAGGGGAACTGATTACTCTCATTCTTTATGTTTGCATGTGTGTGATGGCTCTTCAGAGTTTCATGTTTACTCAAAGAAAAGTTGGTTGTCTTTCTGCCCTGAACAAGGTGCCCTAGTAATCACTGCTGGAGATCAAATCCAGGCATTGAGTGGAGGGCATTACAAGGATGTCATTGGAAAGGCAATATTTAAAGGCAAAAGAGAAGACAACATTTCAATGGCTTTCCTCTATTCTCCTCAAAATGCCAAGAAACTTCAAACAAATATGGAAAGACCTATTTCACTTGGCCAACAAGCCATCTTGGCTGTAATTTTGAACCTTATGTACCACGTTCTAATATTTGTCTTTAAAAAAGTGTGA
Protein sequence

>Msa1312430

MRSLILTPRTMPETTVDFRAPPPSPVASGRRSTVTNNEFLTEFLETSLRVPDLVLPDKIFPKQINDETPPKVDFVALCFHEDEDLIDVVTESIARFGCFQLINHGISPGVVAAAMEVAGGIFTVPLEMRDAVTRSPEKPRGFEEHHDEVEEEEERRELNEEFIWCKNDEELKLKLEGIWPVEYPNFSKKMETLQSRVEKVAEKILDIILKNVKKKLASDGDIIGHRNEVGTLCCIYKHHQDINRNDHWVDSLKYDVIRMLIRGTDYSHSLCLHVCDGSSEFHVYSKKSWLSFCPEQGALVITAGDQIQALSGGHYKDVIGKAIFKGKREDNISMAFLYSPQNAKKLQTNMERPISLGQQAILAVILNLMYHVLIFVFKKV*