Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1317370 | XP_013444703.1 | 85.535 | 878 | 117 | 6 | 1 | 874 | 6 | 877 | 0.0 | 1491 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1317370 | sp|C0LGD6|Y1570_ARATH | 47.749 | 844 | 416 | 14 | 14 | 845 | 16 | 846 | 0.0 | 746 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1317370 | A0A072TPE6 | 85.535 | 878 | 117 | 6 | 1 | 874 | 6 | 877 | 0.0 | 1491 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1278110 | Msa1317370 | 0.835488 | 1.629551e-56 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1317370 | MtrunA17_Chr8g0347661 | 85.535 | 878 | 117 | 6 | 1 | 874 | 6 | 877 | 0.0 | 1491 |
| Msa1317370 | MtrunA17_Chr8g0340931 | 76.731 | 881 | 188 | 10 | 1 | 874 | 1 | 871 | 0.0 | 1364 |
| Msa1317370 | MtrunA17_Chr8g0340881 | 76.424 | 878 | 192 | 9 | 3 | 874 | 8 | 876 | 0.0 | 1352 |
| Msa1317370 | MtrunA17_Chr8g0341151 | 75.399 | 878 | 198 | 9 | 1 | 874 | 2 | 865 | 0.0 | 1325 |
| Msa1317370 | MtrunA17_Chr8g0340871 | 74.011 | 885 | 208 | 10 | 1 | 874 | 8 | 881 | 0.0 | 1292 |
| Msa1317370 | MtrunA17_Chr8g0340891 | 73.606 | 879 | 211 | 11 | 1 | 874 | 6 | 868 | 0.0 | 1285 |
| Msa1317370 | MtrunA17_Chr8g0341011 | 73.948 | 879 | 215 | 7 | 1 | 874 | 6 | 875 | 0.0 | 1275 |
| Msa1317370 | MtrunA17_Chr8g0340911 | 73.121 | 878 | 220 | 10 | 1 | 874 | 6 | 871 | 0.0 | 1274 |
| Msa1317370 | MtrunA17_Chr8g0358831 | 66.938 | 859 | 208 | 13 | 1 | 845 | 14 | 810 | 0.0 | 1090 |
| Msa1317370 | MtrunA17_Chr8g0341101 | 57.853 | 885 | 329 | 18 | 21 | 874 | 51 | 922 | 0.0 | 929 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1317370 | AT4G29990.1 | 46.989 | 847 | 423 | 12 | 12 | 842 | 10 | 846 | 0.0 | 748 |
| Msa1317370 | AT1G05700.1 | 47.749 | 844 | 416 | 14 | 14 | 845 | 16 | 846 | 0.0 | 746 |
| Msa1317370 | AT2G19210.1 | 44.546 | 871 | 456 | 13 | 6 | 858 | 5 | 866 | 0.0 | 745 |
| Msa1317370 | AT2G19230.1 | 46.190 | 840 | 428 | 11 | 15 | 842 | 16 | 843 | 0.0 | 744 |
| Msa1317370 | AT2G19230.2 | 46.190 | 840 | 427 | 12 | 15 | 842 | 16 | 842 | 0.0 | 739 |
| Msa1317370 | AT1G05700.2 | 46.286 | 875 | 414 | 16 | 14 | 845 | 16 | 877 | 0.0 | 732 |
| Msa1317370 | AT1G51850.2 | 43.439 | 884 | 440 | 20 | 15 | 863 | 12 | 870 | 0.0 | 707 |
| Msa1317370 | AT2G19190.1 | 45.169 | 859 | 441 | 14 | 1 | 842 | 1 | 846 | 0.0 | 707 |
| Msa1317370 | AT1G51880.2 | 43.005 | 872 | 464 | 15 | 1 | 860 | 19 | 869 | 0.0 | 703 |
| Msa1317370 | AT1G51880.1 | 43.005 | 872 | 464 | 15 | 1 | 860 | 1 | 851 | 0.0 | 702 |
Find 175 sgRNAs with CRISPR-Local
Find 409 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACATCATTATAACATGG+TGG | 0.683961 | 8_4:-18604014 | None:intergenic |
| AAAACTATTATTGGAGAAGG+AGG | 0.505118 | 8_4:+18602276 | Msa1317370:CDS |
| AAAATCCAAGAGAAATCAAA+AGG | 0.491798 | 8_4:-18601824 | None:intergenic |
| AAAATGAAGATCAAATTGTA+GGG | 0.472146 | 8_4:-18597441 | None:intergenic |
| AAACATCAAGAATACTTATG+AGG | 0.521989 | 8_4:+18600773 | Msa1317370:CDS |
| AAAGCACTAGCAAGATCATC+AGG | 0.509636 | 8_4:+18603690 | Msa1317370:CDS |
| AAAGGGGCACTGACATTTAC+TGG | 0.306860 | 8_4:-18599085 | None:intergenic |
| AAATCCCTATGTATAATGGG+TGG | 0.644529 | 8_4:-18603338 | None:intergenic |
| AAATCTAGTGTGAGAGCTAG+TGG | 0.591650 | 8_4:+18604096 | Msa1317370:three_prime_UTR |
| AAATGAAGATCAAATTGTAG+GGG | 0.666075 | 8_4:-18597440 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGTATTACTTGTGTGTATAG+TGG | + | chr8_4:18597104-18597123 | Msa1317370:intron | 30.0% | |
| TGAAAATAGCTCAAATGTTG+AGG | - | chr8_4:18597203-18597222 | None:intergenic | 30.0% | |
| TGAAATGTAATTTATGCCTG+TGG | - | chr8_4:18597231-18597250 | None:intergenic | 30.0% | |
| TACACCTGTATCTATGAATT+TGG | - | chr8_4:18597258-18597277 | None:intergenic | 30.0% | |
| TGTATAACAATATCTGTAGG+TGG | - | chr8_4:18597293-18597312 | None:intergenic | 30.0% | |
| ACCTACAGATATTGTTATAC+AGG | + | chr8_4:18597292-18597311 | Msa1317370:CDS | 30.0% | |
| TCCTGTATAACAATATCTGT+AGG | - | chr8_4:18597296-18597315 | None:intergenic | 30.0% | |
| ATCAGAGCTTCTTTCTATTA+TGG | + | chr8_4:18597395-18597414 | Msa1317370:CDS | 30.0% | |
| CTCAAGTTACTATTACTAGT+GGG | + | chr8_4:18598132-18598151 | Msa1317370:intron | 30.0% | |
| ACTTACACCAACAGTAAATA+AGG | + | chr8_4:18598766-18598785 | Msa1317370:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 18596947 | 18604264 | 18596947 | ID=Msa1317370;Name=Msa1317370 |
| chr8_4 | mRNA | 18596947 | 18604264 | 18596947 | ID=Msa1317370-mRNA-1;Parent=Msa1317370;Name=Msa1317370-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0.75|0.76|1|1|1|13|251|874 |
| chr8_4 | exon | 18596947 | 18597028 | 18596947 | ID=Msa1317370-mRNA-1:exon:6882;Parent=Msa1317370-mRNA-1 |
| chr8_4 | exon | 18597174 | 18597714 | 18597174 | ID=Msa1317370-mRNA-1:exon:6883;Parent=Msa1317370-mRNA-1 |
| chr8_4 | exon | 18598934 | 18599427 | 18598934 | ID=Msa1317370-mRNA-1:exon:6884;Parent=Msa1317370-mRNA-1 |
| chr8_4 | exon | 18600760 | 18600898 | 18600760 | ID=Msa1317370-mRNA-1:exon:6885;Parent=Msa1317370-mRNA-1 |
| chr8_4 | exon | 18600979 | 18601050 | 18600979 | ID=Msa1317370-mRNA-1:exon:6886;Parent=Msa1317370-mRNA-1 |
| chr8_4 | exon | 18601387 | 18601458 | 18601387 | ID=Msa1317370-mRNA-1:exon:6887;Parent=Msa1317370-mRNA-1 |
| chr8_4 | exon | 18601539 | 18601619 | 18601539 | ID=Msa1317370-mRNA-1:exon:6888;Parent=Msa1317370-mRNA-1 |
| chr8_4 | exon | 18601728 | 18601867 | 18601728 | ID=Msa1317370-mRNA-1:exon:6889;Parent=Msa1317370-mRNA-1 |
| chr8_4 | exon | 18602184 | 18602401 | 18602184 | ID=Msa1317370-mRNA-1:exon:6890;Parent=Msa1317370-mRNA-1 |
| chr8_4 | exon | 18602547 | 18602673 | 18602547 | ID=Msa1317370-mRNA-1:exon:6891;Parent=Msa1317370-mRNA-1 |
| chr8_4 | exon | 18602972 | 18603034 | 18602972 | ID=Msa1317370-mRNA-1:exon:6892;Parent=Msa1317370-mRNA-1 |
| chr8_4 | exon | 18603309 | 18603498 | 18603309 | ID=Msa1317370-mRNA-1:exon:6893;Parent=Msa1317370-mRNA-1 |
| chr8_4 | exon | 18603608 | 18604264 | 18603608 | ID=Msa1317370-mRNA-1:exon:6894;Parent=Msa1317370-mRNA-1 |
| chr8_4 | CDS | 18596947 | 18597028 | 18596947 | ID=Msa1317370-mRNA-1:cds;Parent=Msa1317370-mRNA-1 |
| chr8_4 | CDS | 18597174 | 18597714 | 18597174 | ID=Msa1317370-mRNA-1:cds;Parent=Msa1317370-mRNA-1 |
| chr8_4 | CDS | 18598934 | 18599427 | 18598934 | ID=Msa1317370-mRNA-1:cds;Parent=Msa1317370-mRNA-1 |
| chr8_4 | CDS | 18600760 | 18600898 | 18600760 | ID=Msa1317370-mRNA-1:cds;Parent=Msa1317370-mRNA-1 |
| chr8_4 | CDS | 18600979 | 18601050 | 18600979 | ID=Msa1317370-mRNA-1:cds;Parent=Msa1317370-mRNA-1 |
| chr8_4 | CDS | 18601387 | 18601458 | 18601387 | ID=Msa1317370-mRNA-1:cds;Parent=Msa1317370-mRNA-1 |
| chr8_4 | CDS | 18601539 | 18601619 | 18601539 | ID=Msa1317370-mRNA-1:cds;Parent=Msa1317370-mRNA-1 |
| chr8_4 | CDS | 18601728 | 18601867 | 18601728 | ID=Msa1317370-mRNA-1:cds;Parent=Msa1317370-mRNA-1 |
| chr8_4 | CDS | 18602184 | 18602401 | 18602184 | ID=Msa1317370-mRNA-1:cds;Parent=Msa1317370-mRNA-1 |
| chr8_4 | CDS | 18602547 | 18602673 | 18602547 | ID=Msa1317370-mRNA-1:cds;Parent=Msa1317370-mRNA-1 |
| chr8_4 | CDS | 18602972 | 18603034 | 18602972 | ID=Msa1317370-mRNA-1:cds;Parent=Msa1317370-mRNA-1 |
| chr8_4 | CDS | 18603309 | 18603498 | 18603309 | ID=Msa1317370-mRNA-1:cds;Parent=Msa1317370-mRNA-1 |
| chr8_4 | CDS | 18603608 | 18604013 | 18603608 | ID=Msa1317370-mRNA-1:cds;Parent=Msa1317370-mRNA-1 |
| chr8_4 | three_prime_UTR | 18604014 | 18604264 | 18604014 | ID=Msa1317370-mRNA-1:three_prime_utr;Parent=Msa1317370-mRNA-1 |
| Gene Sequence |
>Msa1317370 ATGAGAATGTTGCTGCATTTTCTTTCCATATTGCTTGGCGTTCTTACTATTTTGGTTTTGATACAAGCTCAAGATCAATCAGGATTCATTAGCATAGATTGTGGTCTACCTCAACATTTGAGCTATTTTCATGAGACCACAGGCATAAATTACATTTCAGATGCCAAATTCATAGATACAGGTGTAAGCAAGAGGATACCACCTACAGATATTGTTATACAGGAACTAGAATATGTGAGGAGCTTTCCTAGTGGAGTGAGAAATTGTTACAGAATAAATATAACAAGTGGTACTAAATATTTAATCAGAGCTTCTTTCTATTATGGAAACTATGATGGTTTAAATAATCCCCTACAATTTGATCTTCATTTTGGAGCTAATGTTTGGGATACAGTGAAGTTCAACAATTTATCACGCTTCATAATCAGAGAGATCATTTACACTCCATCTCTAGATTACATACAACCATGTCTTGTTAACACAGGCAAAGGGACTCCATTCATTTCAGTAATAGAATTGAGGACTTTGAAGAATAAAGCTTATGTCACAGACTCAGCTAAATCATTATCACTCTTTACACGATTTCACATGGGTTCCAAATCCATCAAAAACTTCCGATACCGGTACAAAAATGATGTTTTTGACCGCATCTGGTCTCCTCTTCCTCTTAAATCCAATGATGATTGGGCACGATTAGACACCTCCCTTAATAGTGATGATCTAGATCAGAATGATTTTGAACCACCAACAATTGTGATGAGCAGTGCTGCTACACCAGTAAATGTCAGTGCCCCTTTACAATTTAAATGGTATGCAGATAATATAAATGACCGATACTACCCCTACATGTACTTTAATGAGGTTGAAAATCTGGCACCAAATGAAACTCGAGCGTTTAATATCACATTCAGTGGCTCGTTTTTGAATCAGTTTGGACCTGTGACACCTACATACCGGAAAGTAAGGACCTTATTTAGTACATCACCTTTAACTGGAGGCACAAGCTATCTAATTTCCCTTTTCAAGGTAAAGAATTCAACCCTTCCACCCATCCTCAATGCCATTGAAATTTATAAAGTAAAAGACTTCTCACAACCAGAAACTGAACAAGATGATGTTGATACTATCATAAACATCAAGAATACTTATGAGGTGGCTAGAAATTGGATGGGAGATCCATGTGGTCCTGTGAAGTATATGTGGGAAGGCCTAAACTGCAGTATTAATGGCAATAACATCCAAAGAATCACATCTTTGAATTTGTCTTCAAGTGGATTGACTGGTCAGATAGCATCTTCCATATCAAAGCTCACTATGTTACAGTATTTGGATTTATCAAACAATAGCTTAAAGGGTTCCTTACCTGATTTTCTGACGCAACTGCGGTCACTAAAAGTCTTAAATGTGGGGAAGAACAAACTTACAGGGTTAGTTCCAAGTGGACTCCTTGAGAGATCAAAAGCAGGTTCACTTTCATTGAGTGTGGATGATAATCCTGAACTTTGTATGACGGAATCCTGCAAAAAGAAGAACATTGTTGTACCACTTGTTACATTGTTTTCAACATTAATTGTAATCCTTTTGATTTCTCTTGGATTTTGGATATATAGAAGACAAAAAGCTACATGGTCAAAATCTAAGAAAAGGGATTCAATGAAATCAAAGCATCAAAATTTCAGTTACACTGAAATTCTCAATATTACTGATAACTTCAAAACTATTATTGGAGAAGGAGGATTTGGAAAAGTTTACATTGGCATTTTACAAGATCATACTCAAGTAGCTGTAAAAATTCTTTCAAAATCATCAAAGCAAGGTTATAAGGAATTTCAATCTGAGGCACAACTTCTAACGATTGTTCATCATAGAAGTTTGGTCTCTCTCGTTGGATATTGTGATGAAGGTGAAATTCAAGCATTGATTTATGAGTACATGACTAATGGAAATCTCCAACAACACTTATTTGATGGTAATACAAATATTTTGAAATGGAATGAGAGACTCAAAATTGCGGTTGATGCAGCACATGGATTGGAATATCTGCATGATGGATGTAAACCACCCATTATACATAGGGATTTGAAGCCCGCCAACATATTACTAGACGAGAATATGCATGCCAAGATAGCTGATTTTGGCCTAAGTAGAGCTTTTGGTAACGATATTGATTCTCATATATCCACCCGACCTGCTGGTACACTGGGTTATGTTGATCCTAAATATCAGAGAACAGGAAACACAAATAAAAAAAATGATATCTACAGCTTTGGAATTATTCTATTTGAGTTGATCACCGGTCGAAAAGCACTAGCAAGATCATCAGGTGAATACATTCACATACTTCAATGGGTTATTCCCATAGTTGAAGGGGGAGATATTCAAAATGTTGTTGATTCAAGGTTGGAGGGTGAATTTAGCATCAATTCAGCATGGAAAGCTGTAGAGATTGCCATGTCATGCACATCATCAAATGAAGTTGAAAGACCAGACATGAGCAAAATATTGGTAGAGCTAAAGGAGTGTCTATCTTTTGAGACGGTTCAGATAAATAATGGAAGCACCAGAGCTAGAGATGAATTGGTTTCTGTAGCTACTATATCAGAAACCACTATTTTAGCTAGATAA |
| Protein sequence |
>Msa1317370 MRMLLHFLSILLGVLTILVLIQAQDQSGFISIDCGLPQHLSYFHETTGINYISDAKFIDTGVSKRIPPTDIVIQELEYVRSFPSGVRNCYRINITSGTKYLIRASFYYGNYDGLNNPLQFDLHFGANVWDTVKFNNLSRFIIREIIYTPSLDYIQPCLVNTGKGTPFISVIELRTLKNKAYVTDSAKSLSLFTRFHMGSKSIKNFRYRYKNDVFDRIWSPLPLKSNDDWARLDTSLNSDDLDQNDFEPPTIVMSSAATPVNVSAPLQFKWYADNINDRYYPYMYFNEVENLAPNETRAFNITFSGSFLNQFGPVTPTYRKVRTLFSTSPLTGGTSYLISLFKVKNSTLPPILNAIEIYKVKDFSQPETEQDDVDTIINIKNTYEVARNWMGDPCGPVKYMWEGLNCSINGNNIQRITSLNLSSSGLTGQIASSISKLTMLQYLDLSNNSLKGSLPDFLTQLRSLKVLNVGKNKLTGLVPSGLLERSKAGSLSLSVDDNPELCMTESCKKKNIVVPLVTLFSTLIVILLISLGFWIYRRQKATWSKSKKRDSMKSKHQNFSYTEILNITDNFKTIIGEGGFGKVYIGILQDHTQVAVKILSKSSKQGYKEFQSEAQLLTIVHHRSLVSLVGYCDEGEIQALIYEYMTNGNLQQHLFDGNTNILKWNERLKIAVDAAHGLEYLHDGCKPPIIHRDLKPANILLDENMHAKIADFGLSRAFGNDIDSHISTRPAGTLGYVDPKYQRTGNTNKKNDIYSFGIILFELITGRKALARSSGEYIHILQWVIPIVEGGDIQNVVDSRLEGEFSINSAWKAVEIAMSCTSSNEVERPDMSKILVELKECLSFETVQINNGSTRARDELVSVATISETTILAR* |
