AlfalfaGEDB | Msa1323660

M. sativa cultivar zhongmu-4 / Msa1323660

KEGG: -
GO: GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0021700 GO:0022414 GO:0022622 GO:0023052 GO:0030154 GO:0032501 GO:0032502 GO:0032989 GO:0035556 GO:0036211 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0044706 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051716 GO:0060560 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0071944 GO:0080147 GO:0090558 GO:0090627 GO:0099402 GO:0140096 GO:1901564 GO:1905392
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1323660	RHN40584.1	97.159	528	9	2	1	526	1	524	0.0	1062

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1323660	sp\|Q1PFB9\|AGC17_ARATH	70.264	454	104	7	96	526	110	555	0.0	642

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1323660	A0A396GLY8	97.159	528	9	2	1	526	1	524	0.0	1062

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1242590	Msa1323660	0.850399	1.741835e-60	-8.615850e-47
Msa1284330	Msa1323660	0.836918	7.058876e-57	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1323660	MtrunA17_Chr8g0356291	97.159	528	9	2	1	526	1	524	0.0	1062
Msa1323660	MtrunA17_Chr2g0309421	65.549	537	136	10	34	526	40	571	0.0	690
Msa1323660	MtrunA17_Chr2g0290691	63.741	433	143	6	103	526	177	604	0.0	565
Msa1323660	MtrunA17_Chr7g0250631	59.861	431	156	8	105	526	339	761	0.0	527
Msa1323660	MtrunA17_Chr8g0335351	60.460	435	151	7	105	526	345	771	1.49e-179	524
Msa1323660	MtrunA17_Chr7g0227331	64.359	390	122	6	103	480	205	589	3.23e-176	510
Msa1323660	MtrunA17_Chr6g0449401	56.954	453	150	7	69	480	202	650	2.09e-171	500
Msa1323660	MtrunA17_Chr7g0266031	51.378	508	215	12	4	485	347	848	1.18e-156	468
Msa1323660	MtrunA17_Chr3g0080101	47.977	519	235	10	19	526	308	802	4.58e-156	464
Msa1323660	MtrunA17_Chr1g0179101	58.122	394	150	6	105	490	445	831	1.81e-154	462

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa1323660	AT1G79250.1	70.264	454	104	7	96	526	110	555	642
Msa1323660	AT1G79250.3	70.264	454	104	7	96	526	110	555	642
Msa1323660	AT1G79250.2	70.264	454	104	7	96	526	110	555	642
Msa1323660	AT1G79250.4	70.264	454	104	7	96	526	110	555	642
Msa1323660	AT3G12690.1	65.890	472	133	7	68	526	121	577	612
Msa1323660	AT3G12690.3	65.890	472	133	7	68	526	121	577	612
Msa1323660	AT3G12690.2	65.890	472	133	7	68	526	121	577	612
Msa1323660	AT1G16440.1	69.159	428	112	9	105	526	86	499	589
Msa1323660	AT5G47750.1	61.772	429	151	7	105	526	164	586	534
Msa1323660	AT5G55910.2	61.949	431	145	7	103	526	80	498	527

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Find 110 sgRNAs with CRISPR-Local

Find 276 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCAGATTTCGGTCTCA+TGG	0.532025	8_4:-32152900	Msa1323660:CDS
AAATGAGAAGTATTATTGTT+TGG	0.413008	8_4:-32153524	Msa1323660:CDS
AACAATCAAGAAGCAATAGA+TGG	0.436045	8_4:-32154030	Msa1323660:CDS
AACGGTTCAGGTATAATCGG+AGG	0.648560	8_4:+32152482	None:intergenic
AACGTCCTAGTCCGAGACAA+TGG	0.615612	8_4:-32153121	Msa1323660:CDS
AAGAGAATTGCTTATAAAAG+AGG	0.460267	8_4:-32152572	Msa1323660:CDS
ACAACACCGTTTAAAGGTAA+TGG	0.454704	8_4:-32152710	Msa1323660:CDS
ACATACTGACTAAAATCAAA+CGG	0.525447	8_4:+32152464	None:intergenic
ACCAGCACCACCACATCCAT+TGG	0.494567	8_4:-32153016	Msa1323660:CDS
ACGACGAACATGCAGTACAA+GGG	0.700852	8_4:-32152985	Msa1323660:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACAATCAAGAAGCAATAGA+TGG	-	chr8_4:32152445-32152464	Msa1323660:CDS	30.0%
GTGTATCTTGTTGAACTTAA+AGG	-	chr8_4:32152787-32152806	Msa1323660:CDS	30.0%
TGTATACTATTCCTAACATG+TGG	+	chr8_4:32153317-32153336	None:intergenic	30.0%
CAATTAACACCTTCGAAAAA+TGG	+	chr8_4:32153951-32153970	None:intergenic	30.0%
AACAATCAAGAAGCAATAGA+TGG	-	chr8_4:32152445-32152464	Msa1323660:CDS	30.0%
GTGTATCTTGTTGAACTTAA+AGG	-	chr8_4:32152787-32152806	Msa1323660:CDS	30.0%
TGTATACTATTCCTAACATG+TGG	+	chr8_4:32153317-32153336	None:intergenic	30.0%
CAATTAACACCTTCGAAAAA+TGG	+	chr8_4:32153951-32153970	None:intergenic	30.0%
ATAAACCAACATCATCACCA+AGG	-	chr8_4:32152503-32152522	Msa1323660:CDS	35.0%
CTTGAAATCATTCTTGGACT+TGG	+	chr8_4:32152586-32152605	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	32152349	32154148	32152349	ID=Msa1323660;Name=Msa1323660
chr8_4	mRNA	32152349	32154148	32152349	ID=Msa1323660-mRNA-1;Parent=Msa1323660;Name=Msa1323660-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0\|0\|0\|1\|1\|1\|2\|0\|526
chr8_4	exon	32153442	32154148	32153442	ID=Msa1323660-mRNA-1:exon:10441;Parent=Msa1323660-mRNA-1
chr8_4	exon	32152349	32153222	32152349	ID=Msa1323660-mRNA-1:exon:10440;Parent=Msa1323660-mRNA-1
chr8_4	CDS	32153442	32154148	32153442	ID=Msa1323660-mRNA-1:cds;Parent=Msa1323660-mRNA-1
chr8_4	CDS	32152349	32153222	32152349	ID=Msa1323660-mRNA-1:cds;Parent=Msa1323660-mRNA-1

Gene Sequence

>Msa1323660

ATGCAGATGAGTGACAACAGCTTATCGGAAGAAGCTCCCAAAACAGATCAAAGTTGTGCAAATGATCATACAGAAAATGATGATACTAAACAAGTGAACAATCAAGAAGCAATAGATGGTTTCACAAATCAATACAATGCAAAGAAAACTACAAATAAACCAACATCATCACCAAGGCCACAAAAGTTAGAATCACTTATGAGAAATTTCTCTGAATTTTCCACAACTCCAAGACCAAGTCCAAGAATGATTTCAAGGAGTGGACCGAATTCACGTAGCGGTAGTCTAGAAAGCACAAGTGCACCAATATTTAAGCCTCATACAGGAGGTGATGTGAGATGGGATGCAATCAACATGGTTTCAAGAGGGTCAAGTTCAAATCTTAACTTTGGTCATTTTCGGCTTGTGAAGCGTGTTGGTTACGGTGACATTGGAAGTGTGTATCTTGTTGAACTTAAAGGGACAGGAACATTCTATGCTATGAAATTAATGGATAAGGCTTCACTTGCTAGCAGAAATAAGCTCCTTAGAGCACATACTGAAAGGGAGATTCTTGGACTTCTTGATCATCCTTTCTTGCCAACTTTGTATTCCTATTTTGAAAATGAGAAGTATTATTGTTTGGTCATGGAGTTTTGTAGTTGTGGTAGTTTGCATAACCTCAGAATGAAGCAACCCAACAAGCATTTCACTGAAAATGCTGCAAGGTTTTATTGCTCAGAGATTCTATTAGCAATGGAATACCTCCACATGTTAGGAATAGTATACAGAGACTTAAAACCAGAAAACGTCCTAGTCCGAGACAATGGTCACATAATGCTATCAGATTTCGACTTATCCCTCCGTTGTTCTGTCAGCCCCACCCTCGTCAAATCCTCCTCCGCCCACATAACCAGCACCACCACATCCATTGGAATCCTGGACGACGAACATGCAGTACAAGGGTGCATGCAACCATCAAGTTTCTTCTTCCCAAGAATCCTCCCATCCAAAAGAAACCGAAAATCAAAATCAGATTTCGGTCTCATGGTAGGTGGCCGTCTTCCAGAATTAATGGCAGAACCAACTAACGTCCGATCAATGTCATTCGTTGGAACACATGAGTATCTTGCTCCAGAAATCGTACGTGGTGAAGGACACGGTAGTGCTGTTGATTGGTGGACATTTGGGATATTTTTATACGAGCTATTATACGGAACAACACCGTTTAAAGGTAATGGAAACCGCGCTACGCTTTTTAATGTTGTAGGACAGCCTTTAAGGTTTCCTGACCATCCTTCCGCTAGTCCAGTAGCTAGGGATCTTATTAGAGGATTGTTGGTTAAAGAGCCTCATAAGAGAATTGCTTATAAAAGAGGTGCTACTGAGATTAAACAACATCCATTTTTCGAAGGTGTTAATTGGGCTTTGATTCGAAGTTCTACTCCTCCGATTATACCTGAACCGTTTGATTTTAGTCAGTATGTTAGTAAGGATACTGTTGTGGCTGCTGCTGATAATAAGAAGATTGTTGATATTGTTAGTGATAAAAATAGTAAGATGCAGCATGATTCTTCTTATGAAGATTTTGAGTATTTTTAG

Protein sequence

>Msa1323660

MQMSDNSLSEEAPKTDQSCANDHTENDDTKQVNNQEAIDGFTNQYNAKKTTNKPTSSPRPQKLESLMRNFSEFSTTPRPSPRMISRSGPNSRSGSLESTSAPIFKPHTGGDVRWDAINMVSRGSSSNLNFGHFRLVKRVGYGDIGSVYLVELKGTGTFYAMKLMDKASLASRNKLLRAHTEREILGLLDHPFLPTLYSYFENEKYYCLVMEFCSCGSLHNLRMKQPNKHFTENAARFYCSEILLAMEYLHMLGIVYRDLKPENVLVRDNGHIMLSDFDLSLRCSVSPTLVKSSSAHITSTTTSIGILDDEHAVQGCMQPSSFFFPRILPSKRNRKSKSDFGLMVGGRLPELMAEPTNVRSMSFVGTHEYLAPEIVRGEGHGSAVDWWTFGIFLYELLYGTTPFKGNGNRATLFNVVGQPLRFPDHPSASPVARDLIRGLLVKEPHKRIAYKRGATEIKQHPFFEGVNWALIRSSTPPIIPEPFDFSQYVSKDTVVAAADNKKIVDIVSDKNSKMQHDSSYEDFEYF*