AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1334320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1334320 MtrunA17_Chr4g0074101 85.204 196 12 1 1 179 1 196 4.14e-117 330
Msa1334320 MtrunA17_Chr1g0169761 83.240 179 30 0 1 179 1 179 8.85e-111 313
Msa1334320 MtrunA17_Chr8g0376821 86.228 167 23 0 1 167 1 167 6.91e-105 298
Msa1334320 MtrunA17_Chr1g0180591 78.212 179 39 0 1 179 1 179 1.19e-100 287
Msa1334320 MtrunA17_Chr1g0175601 77.222 180 40 1 1 179 1 180 6.97e-99 283
Msa1334320 MtrunA17_Chr1g0175611 78.698 169 36 0 1 169 1 169 2.70e-95 273
Msa1334320 MtrunA17_Chr8g0357771 60.571 175 69 0 1 175 1 175 4.48e-74 220
Msa1334320 MtrunA17_Chr8g0356071 58.333 168 64 2 1 168 1 162 9.23e-64 193
Msa1334320 MtrunA17_Chr4g0052061 40.854 164 85 3 1 164 1 152 1.03e-34 119
Msa1334320 MtrunA17_Chr3g0119901 40.588 170 82 4 1 157 8 171 3.53e-31 112
Msa1334320 MtrunA17_Chr3g0099411 41.304 138 78 2 2 136 9 146 7.47e-30 108
Msa1334320 MtrunA17_Chr4g0052081 43.357 143 76 3 1 140 1 141 1.43e-29 107
Msa1334320 MtrunA17_Chr3g0085241 41.176 153 79 4 1 148 40 186 2.38e-27 103
Msa1334320 MtrunA17_Chr1g0206991 46.535 101 52 1 2 100 9 109 4.40e-27 100
Msa1334320 MtrunA17_Chr1g0206721 47.525 101 51 1 2 100 9 109 1.39e-26 100
Msa1334320 MtrunA17_Chr3g0099421 47.525 101 51 1 2 100 34 134 7.69e-26 99.8
Msa1334320 MtrunA17_Chr5g0419601 35.329 167 90 4 1 152 8 171 2.29e-25 97.8
Msa1334320 MtrunA17_Chr1g0149391 40.650 123 70 2 2 122 9 130 2.77e-25 97.8
Msa1334320 MtrunA17_Chr1g0206701 46.535 101 52 1 2 100 9 109 2.92e-25 97.4
Msa1334320 MtrunA17_Chr8g0355091 47.525 101 51 1 2 100 9 109 1.11e-24 95.9
Msa1334320 MtrunA17_Chr2g0333981 41.964 112 63 1 1 112 11 120 1.36e-24 94.4
Msa1334320 MtrunA17_Chr1g0188671 45.545 101 53 1 2 100 47 147 2.54e-24 95.9
Msa1334320 MtrunA17_Chr2g0292791 35.714 154 87 2 2 143 9 162 2.91e-24 95.1
Msa1334320 MtrunA17_Chr2g0300421 45.545 101 53 1 2 100 9 109 3.33e-24 93.2
Msa1334320 MtrunA17_Chr1g0208581 44.554 101 54 1 2 100 9 109 5.02e-24 94.0
Msa1334320 MtrunA17_Chr1g0208561 45.545 101 53 1 2 100 9 109 5.89e-24 94.0
Msa1334320 MtrunA17_Chr3g0089141 45.545 101 53 1 2 100 9 109 8.71e-24 93.6
Msa1334320 MtrunA17_Chr1g0187361 56.164 73 32 0 2 74 9 81 2.70e-23 92.4
Msa1334320 MtrunA17_Chr2g0325971 44.144 111 59 2 2 110 9 118 5.37e-23 91.3
Msa1334320 MtrunA17_Chr5g0422421 53.521 71 33 0 2 72 9 79 1.82e-22 90.1
Msa1334320 MtrunA17_Chr4g0018951 37.121 132 71 2 14 139 24 149 2.21e-22 88.2
Msa1334320 MtrunA17_Chr5g0422941 50.649 77 38 0 2 78 9 85 3.97e-22 89.4
Msa1334320 MtrunA17_Chr3g0089151 47.436 78 41 0 2 79 9 86 4.69e-22 86.7
Msa1334320 MtrunA17_Chr1g0206691 37.681 138 74 2 18 143 8 145 5.77e-22 88.6
Msa1334320 MtrunA17_Chr2g0317451 50.000 78 39 0 2 79 9 86 1.06e-21 88.2
Msa1334320 MtrunA17_Chr2g0317481 50.000 78 39 0 2 79 9 86 1.10e-21 88.2
Msa1334320 MtrunA17_Chr8g0379021 42.342 111 57 3 2 110 12 117 1.82e-21 86.3
Msa1334320 MtrunA17_Chr2g0300441 43.564 101 55 1 2 100 9 109 6.51e-21 86.3
Msa1334320 MtrunA17_Chr2g0284911 41.346 104 58 1 1 104 16 116 7.88e-20 83.6
Msa1334320 MtrunA17_Chr4g0020221 50.725 69 34 0 1 69 1 69 1.44e-19 82.8
Msa1334320 MtrunA17_Chr5g0404951 50.000 64 32 0 1 64 1 64 5.04e-19 80.9
Msa1334320 MtrunA17_Chr1g0187351 60.000 55 22 0 18 72 8 62 7.62e-19 76.3
Msa1334320 MtrunA17_Chr8g0380021 39.423 104 60 1 1 104 17 117 1.09e-18 80.5
Msa1334320 MtrunA17_Chr1g0173161 46.154 78 39 1 1 78 1 75 1.87e-18 75.9
Msa1334320 MtrunA17_Chr7g0238991 48.571 70 36 0 10 79 29 98 2.77e-18 79.3
Msa1334320 MtrunA17_Chr2g0279821 39.091 110 66 1 1 110 1 109 4.21e-18 78.6
Msa1334320 MtrunA17_Chr3g0097101 50.725 69 34 0 1 69 16 84 8.93e-18 77.8
Msa1334320 MtrunA17_Chr5g0427401 47.222 72 38 0 1 72 1 72 9.17e-18 77.4
Msa1334320 MtrunA17_Chr3g0077501 50.725 69 34 0 1 69 1 69 1.13e-17 77.4
Msa1334320 MtrunA17_Chr4g0071601 50.000 80 40 0 1 80 11 90 1.52e-17 76.3
Msa1334320 MtrunA17_Chr8g0351041 43.182 88 45 1 1 83 1 88 1.87e-17 75.5
Msa1334320 MtrunA17_Chr4g0061671 34.351 131 71 3 1 116 1 131 5.41e-17 74.7
Msa1334320 MtrunA17_Chr8g0351051 47.826 69 36 0 1 69 1 69 5.66e-17 75.9
Msa1334320 MtrunA17_Chr7g0246431 36.538 104 63 1 1 104 1 101 1.59e-16 73.9
Msa1334320 MtrunA17_Chr5g0427921 51.667 60 29 0 1 60 1 60 1.60e-16 70.1
Msa1334320 MtrunA17_Chr8g0351031 43.678 87 44 1 1 82 1 87 1.84e-16 73.9
Msa1334320 MtrunA17_Chr4g0049541 46.377 69 37 0 1 69 1 69 2.79e-16 73.6
Msa1334320 MtrunA17_Chr4g0057151 46.377 69 37 0 1 69 1 69 5.16e-16 72.4
Msa1334320 MtrunA17_Chr1g0164791 47.887 71 35 1 1 69 1 71 6.96e-16 72.8
Msa1334320 MtrunA17_Chr6g0474371 33.333 138 84 4 1 134 1 134 7.71e-16 71.6
Msa1334320 MtrunA17_Chr4g0049901 49.231 65 33 0 1 65 27 91 8.27e-16 72.4
Msa1334320 MtrunA17_Chr4g0044071 56.897 58 25 0 1 58 1 58 9.78e-16 69.7
Msa1334320 MtrunA17_Chr3g0111841 56.250 48 21 0 18 65 12 59 1.05e-15 68.2
Msa1334320 MtrunA17_Chr3g0111931 49.123 57 29 0 18 74 6 62 1.29e-15 68.9
Msa1334320 MtrunA17_Chr1g0204111 45.070 71 39 0 1 71 17 87 1.29e-15 72.4
Msa1334320 MtrunA17_Chr5g0411691 46.377 69 37 0 1 69 1 69 1.73e-15 71.6
Msa1334320 MtrunA17_Chr5g0425191 56.897 58 25 0 1 58 1 58 2.19e-15 68.6
Msa1334320 MtrunA17_Chr5g0420091 50.877 57 28 0 1 57 1 57 6.88e-15 69.7
Msa1334320 MtrunA17_Chr7g0246381 46.377 69 32 1 1 64 1 69 8.61e-15 65.9
Msa1334320 MtrunA17_Chr7g0220111 50.877 57 28 0 1 57 1 57 1.00e-14 69.7
Msa1334320 MtrunA17_Chr2g0308251 40.845 71 41 1 1 70 1 71 1.03e-14 66.2
Msa1334320 MtrunA17_Chr3g0122251 40.206 97 53 1 1 97 1 92 1.14e-14 69.7
Msa1334320 MtrunA17_Chr8g0377561 50.877 57 28 0 1 57 1 57 1.39e-14 66.2
Msa1334320 MtrunA17_Chr3g0142191 48.333 60 31 0 1 60 1 60 1.68e-14 68.9
Msa1334320 MtrunA17_Chr5g0420081 47.368 57 30 0 1 57 1 57 1.72e-14 68.9
Msa1334320 MtrunA17_Chr3g0134451 45.070 71 38 1 1 70 1 71 1.80e-14 69.7
Msa1334320 MtrunA17_Chr4g0061691 40.000 70 41 1 1 69 1 70 2.22e-14 68.6
Msa1334320 MtrunA17_Chr4g0018421 45.833 72 38 1 1 71 1 72 2.95e-14 67.8
Msa1334320 MtrunA17_Chr5g0412271 49.123 57 29 0 1 57 1 57 3.13e-14 68.2
Msa1334320 MtrunA17_Chr5g0412461 49.123 57 29 0 1 57 1 57 3.39e-14 68.2
Msa1334320 MtrunA17_Chr6g0455511 50.877 57 28 0 1 57 1 57 4.26e-14 68.2
Msa1334320 MtrunA17_Chr7g0220121 35.227 88 51 2 1 82 1 88 4.37e-14 68.2
Msa1334320 MtrunA17_Chr1g0166611 65.909 44 15 0 15 58 19 62 4.85e-14 65.9
Msa1334320 MtrunA17_Chr8g0366231 46.552 58 31 0 1 58 1 58 7.10e-14 67.4
Msa1334320 MtrunA17_Chr1g0159661 32.432 111 64 3 1 105 1 106 8.49e-14 66.2
Msa1334320 MtrunA17_Chr3g0125241 38.462 65 40 0 1 65 26 90 1.69e-13 65.9
Msa1334320 MtrunA17_Chr5g0417661 50.000 54 27 0 1 54 56 109 2.65e-13 63.5
Msa1334320 MtrunA17_Chr3g0111831 48.077 52 27 0 18 69 20 71 2.19e-12 60.1
Msa1334320 MtrunA17_Chr8g0345001 38.824 85 44 1 3 87 19 95 7.38e-12 60.8
Msa1334320 MtrunA17_Chr4g0060841 38.961 77 45 1 1 75 1 77 9.54e-12 62.4
Msa1334320 MtrunA17_Chr3g0093131 33.684 95 60 2 19 112 36 128 1.75e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1334320 AT2G34440.1 44.767 172 83 5 1 161 1 171 4.40e-41 136
Msa1334320 AT3G66656.1 42.373 177 87 4 1 162 1 177 2.83e-32 114
Msa1334320 AT2G24840.1 35.329 167 87 3 1 150 62 224 1.17e-29 110
Msa1334320 AT5G60440.1 39.490 157 76 3 2 154 7 148 1.95e-27 105
Msa1334320 AT1G01530.1 42.143 140 77 2 1 140 6 141 4.90e-25 97.4
Msa1334320 AT3G57230.2 40.909 110 64 1 1 110 1 109 4.05e-21 84.7
Msa1334320 AT1G65360.1 37.168 113 71 0 1 113 6 118 6.65e-21 86.3
Msa1334320 AT5G13790.2 42.857 98 49 1 1 98 1 91 6.76e-21 87.0
Msa1334320 AT1G47760.1 50.649 77 36 1 1 77 1 75 9.00e-21 85.1
Msa1334320 AT4G36590.1 42.969 128 61 5 2 120 7 131 2.08e-20 85.5
Msa1334320 AT3G57230.1 40.909 110 64 1 1 110 1 109 2.10e-20 85.1
Msa1334320 AT3G57230.4 40.909 110 64 1 1 110 1 109 2.10e-20 85.1
Msa1334320 AT1G72350.1 43.846 130 62 4 2 131 43 161 4.33e-20 84.0
Msa1334320 AT3G04100.1 35.915 142 68 4 2 138 14 137 1.93e-19 82.0
Msa1334320 AT5G13790.1 50.725 69 34 0 1 69 1 69 2.31e-19 83.2
Msa1334320 AT3G57390.3 50.725 69 34 0 1 69 1 69 8.17e-19 80.9
Msa1334320 AT3G61120.1 47.826 69 36 0 1 69 1 69 1.19e-18 80.5
Msa1334320 AT3G57390.1 50.725 69 34 0 1 69 1 69 1.50e-18 80.5
Msa1334320 AT4G37940.3 33.333 156 93 3 1 156 1 145 2.68e-18 78.2
Msa1334320 AT4G37940.1 39.091 110 66 1 1 110 1 109 4.23e-18 79.0
Msa1334320 AT4G37940.4 35.821 134 80 2 1 134 1 128 4.30e-18 78.6
Msa1334320 AT4G22950.2 49.275 69 35 0 1 69 1 69 5.55e-18 77.4
Msa1334320 AT5G51860.2 44.578 83 46 0 1 83 1 83 5.98e-18 77.8
Msa1334320 AT5G51860.1 44.578 83 46 0 1 83 1 83 6.18e-18 78.2
Msa1334320 AT5G51870.1 37.037 108 58 2 1 104 1 102 8.64e-18 77.8
Msa1334320 AT4G09960.6 50.725 69 34 0 1 69 2 70 8.85e-18 78.2
Msa1334320 AT4G09960.1 50.725 69 34 0 1 69 1 69 9.36e-18 78.2
Msa1334320 AT4G09960.2 50.725 69 34 0 1 69 1 69 9.55e-18 77.8
Msa1334320 AT5G51870.2 37.037 108 58 2 1 104 1 102 1.00e-17 76.6
Msa1334320 AT4G09960.5 50.725 69 34 0 1 69 11 79 1.27e-17 77.8
Msa1334320 AT5G51870.5 37.037 108 58 2 1 104 24 125 1.45e-17 76.6
Msa1334320 AT5G51870.4 37.037 108 58 2 1 104 1 102 1.50e-17 77.4
Msa1334320 AT2G22630.1 37.273 110 68 1 1 110 1 109 1.53e-17 77.4
Msa1334320 AT2G22630.2 37.273 110 68 1 1 110 1 109 1.53e-17 77.4
Msa1334320 AT2G45650.1 49.275 69 35 0 1 69 1 69 1.66e-17 77.8
Msa1334320 AT4G09960.3 50.725 69 34 0 1 69 27 95 1.96e-17 77.8
Msa1334320 AT4G09960.4 50.725 69 34 0 1 69 96 164 1.98e-17 78.6
Msa1334320 AT5G51870.3 37.037 108 58 2 1 104 11 112 2.98e-17 76.6
Msa1334320 AT2G45660.2 35.185 108 67 2 1 108 1 105 3.21e-17 75.1
Msa1334320 AT3G58780.1 38.679 106 62 1 1 106 16 118 3.28e-17 77.0
Msa1334320 AT3G58780.5 38.679 106 62 1 1 106 22 124 3.51e-17 77.0
Msa1334320 AT2G42830.1 38.679 106 62 1 1 106 16 118 3.88e-17 76.6
Msa1334320 AT2G42830.2 38.679 106 62 1 1 106 16 118 4.04e-17 76.6
Msa1334320 AT1G29962.1 44.444 72 40 0 2 73 8 79 4.71e-17 75.1
Msa1334320 AT4G22950.3 49.275 69 35 0 1 69 1 69 5.07e-17 75.9
Msa1334320 AT4G22950.1 49.275 69 35 0 1 69 1 69 5.07e-17 75.9
Msa1334320 AT3G58780.4 38.679 106 62 1 1 106 16 118 6.02e-17 76.6
Msa1334320 AT5G65070.4 44.872 78 41 1 1 78 1 76 7.52e-17 73.6
Msa1334320 AT1G28460.1 44.928 69 38 0 2 70 8 76 1.11e-16 74.3
Msa1334320 AT2G45660.1 35.185 108 67 2 1 108 1 105 1.76e-16 74.3
Msa1334320 AT5G65070.1 51.724 58 28 0 1 58 1 58 2.14e-16 73.9
Msa1334320 AT5G20240.2 40.000 100 54 3 1 100 1 94 2.45e-16 72.4
Msa1334320 AT5G62165.17 33.654 104 66 1 1 104 1 101 3.12e-16 72.4
Msa1334320 AT5G62165.12 33.654 104 66 1 1 104 1 101 3.12e-16 72.4
Msa1334320 AT5G62165.11 33.654 104 66 1 1 104 1 101 3.12e-16 72.4
Msa1334320 AT5G65070.3 50.769 65 30 1 1 63 1 65 3.42e-16 73.9
Msa1334320 AT3G58780.2 43.023 86 36 1 1 73 16 101 3.45e-16 73.9
Msa1334320 AT5G10140.2 52.632 57 27 0 1 57 1 57 3.90e-16 72.4
Msa1334320 AT1G17310.1 58.621 58 22 1 17 74 63 118 4.08e-16 73.6
Msa1334320 AT5G62165.6 33.654 104 66 1 1 104 1 101 4.64e-16 72.0
Msa1334320 AT5G62165.9 33.654 104 66 1 1 104 1 101 4.64e-16 72.0
Msa1334320 AT5G62165.7 33.654 104 66 1 1 104 1 101 4.64e-16 72.0
Msa1334320 AT5G62165.8 33.654 104 66 1 1 104 1 101 4.64e-16 72.0
Msa1334320 AT5G62165.13 33.654 104 66 1 1 104 1 101 4.64e-16 72.0
Msa1334320 AT1G17310.2 58.621 58 22 1 17 74 74 129 5.23e-16 73.2
Msa1334320 AT5G62165.14 41.667 84 45 1 1 80 1 84 5.44e-16 70.5
Msa1334320 AT5G10140.4 52.632 57 27 0 1 57 1 57 6.18e-16 72.0
Msa1334320 AT5G10140.3 51.724 58 28 0 1 58 1 58 6.89e-16 72.0
Msa1334320 AT5G65070.2 51.724 58 28 0 1 58 1 58 7.16e-16 73.2
Msa1334320 AT5G10140.1 52.632 57 27 0 1 57 1 57 8.22e-16 72.0
Msa1334320 AT4G24540.1 33.028 109 69 1 1 105 1 109 8.59e-16 72.4
Msa1334320 AT5G20240.1 40.000 100 54 3 1 100 1 94 9.23e-16 72.4
Msa1334320 AT1G77980.1 52.113 71 33 1 1 70 1 71 1.08e-15 73.9
Msa1334320 AT5G65070.5 51.724 58 28 0 1 58 1 58 1.21e-15 72.0
Msa1334320 AT4G11880.2 53.448 58 27 0 1 58 1 58 1.28e-15 71.2
Msa1334320 AT4G11880.3 53.448 58 27 0 1 58 1 58 1.28e-15 71.2
Msa1334320 AT5G62165.3 44.928 69 38 0 1 69 1 69 1.39e-15 72.0
Msa1334320 AT5G62165.1 44.928 69 38 0 1 69 1 69 1.39e-15 72.0
Msa1334320 AT5G62165.2 44.928 69 38 0 1 69 1 69 1.39e-15 72.0
Msa1334320 AT5G62165.10 41.667 84 45 1 1 80 1 84 1.52e-15 71.6
Msa1334320 AT5G62165.4 41.667 84 45 1 1 80 1 84 1.52e-15 71.6
Msa1334320 AT2G22540.3 44.928 69 38 0 1 69 1 69 1.53e-15 72.4
Msa1334320 AT2G22540.1 44.928 69 38 0 1 69 1 69 1.53e-15 72.4
Msa1334320 AT4G18960.1 48.529 68 35 0 2 69 18 85 1.76e-15 72.4
Msa1334320 AT1G71692.1 47.222 72 37 1 1 71 1 72 2.20e-15 71.2
Msa1334320 AT2G14210.1 53.448 58 27 0 1 58 1 58 2.60e-15 71.6
Msa1334320 AT3G58780.3 54.237 59 27 0 1 59 16 74 2.75e-15 72.0
Msa1334320 AT1G22130.1 50.704 71 34 1 1 70 1 71 3.03e-15 72.4
Msa1334320 AT2G14210.2 53.448 58 27 0 1 58 1 58 3.24e-15 71.2
Msa1334320 AT3G30260.1 38.053 113 62 3 1 105 1 113 3.91e-15 71.2
Msa1334320 AT5G65080.3 41.026 78 44 1 1 76 8 85 3.94e-15 70.1
Msa1334320 AT4G11880.4 53.448 58 27 0 1 58 1 58 3.99e-15 70.9
Msa1334320 AT4G11880.1 53.448 58 27 0 1 58 1 58 3.99e-15 70.9
Msa1334320 AT1G46408.1 38.931 131 66 6 1 118 1 130 4.07e-15 71.6
Msa1334320 AT2G03710.3 54.386 57 26 0 1 57 1 57 4.37e-15 70.1
Msa1334320 AT3G54340.1 39.286 84 36 1 1 69 1 84 4.41e-15 70.9
Msa1334320 AT1G77950.3 37.209 172 87 10 1 155 1 168 4.78e-15 71.2
Msa1334320 AT1G77950.2 37.209 172 87 10 1 155 1 168 4.78e-15 71.2
Msa1334320 AT1G77950.1 37.209 172 87 10 1 155 1 168 4.78e-15 71.2
Msa1334320 AT1G77950.4 37.209 172 87 10 1 155 1 168 4.78e-15 71.2
Msa1334320 AT2G22540.2 52.727 55 26 0 1 55 1 55 1.57e-14 69.3
Msa1334320 AT5G65060.3 54.545 55 25 0 1 55 1 55 1.71e-14 68.6
Msa1334320 AT5G65050.2 52.727 55 26 0 1 55 1 55 1.86e-14 68.2
Msa1334320 AT5G23260.2 57.895 57 24 0 1 57 1 57 1.89e-14 69.3
Msa1334320 AT2G03710.2 54.386 57 26 0 1 57 1 57 1.94e-14 69.7
Msa1334320 AT5G23260.4 57.895 57 24 0 1 57 17 73 2.06e-14 69.7
Msa1334320 AT2G03710.1 54.386 57 26 0 1 57 1 57 2.07e-14 69.3
Msa1334320 AT5G23260.3 57.895 57 24 0 1 57 1 57 2.22e-14 68.9
Msa1334320 AT5G23260.1 57.895 57 24 0 1 57 17 73 2.23e-14 69.3
Msa1334320 AT5G65060.2 54.545 55 25 0 1 55 1 55 2.64e-14 67.8
Msa1334320 AT5G65060.1 54.545 55 25 0 1 55 1 55 2.82e-14 68.2
Msa1334320 AT1G24260.2 38.144 97 55 1 1 97 1 92 2.98e-14 68.9
Msa1334320 AT5G15800.1 50.877 57 28 0 1 57 1 57 3.14e-14 68.9
Msa1334320 AT3G02310.2 50.877 57 28 0 1 57 1 57 3.56e-14 67.8
Msa1334320 AT1G26310.1 48.276 58 30 0 1 58 1 58 3.63e-14 68.9
Msa1334320 AT1G24260.3 38.144 97 55 1 1 97 1 92 3.70e-14 68.6
Msa1334320 AT5G15800.2 50.877 57 28 0 1 57 1 57 4.80e-14 68.6
Msa1334320 AT1G69120.2 48.276 58 30 0 1 58 1 58 4.93e-14 68.2
Msa1334320 AT5G60910.1 47.368 57 30 0 1 57 1 57 5.74e-14 68.2
Msa1334320 AT5G65050.1 52.727 55 26 0 1 55 1 55 5.81e-14 67.0
Msa1334320 AT5G65050.3 52.727 55 26 0 1 55 1 55 6.01e-14 67.0
Msa1334320 AT5G65050.5 52.727 55 26 0 1 55 1 55 6.06e-14 67.0
Msa1334320 AT1G24260.1 50.877 57 28 0 1 57 1 57 7.42e-14 67.8
Msa1334320 AT4G37435.1 28.261 184 85 4 1 150 1 171 7.67e-14 66.6
Msa1334320 AT3G02310.1 50.877 57 28 0 1 57 1 57 7.88e-14 67.8
Msa1334320 AT5G65080.2 43.478 69 38 1 1 69 8 75 8.50e-14 67.0
Msa1334320 AT1G69120.1 48.276 58 30 0 1 58 1 58 9.91e-14 67.4
Msa1334320 AT1G18750.4 42.857 77 42 1 1 75 1 77 1.16e-13 68.2
Msa1334320 AT1G18750.3 42.857 77 42 1 1 75 1 77 1.19e-13 68.2
Msa1334320 AT1G18750.1 42.857 77 42 1 1 75 1 77 1.33e-13 68.2
Msa1334320 AT1G69540.1 43.421 76 42 1 1 75 1 76 2.86e-13 67.0
Msa1334320 AT1G69540.2 43.421 76 42 1 1 75 1 76 3.34e-13 67.0
Msa1334320 AT1G28450.1 38.356 73 45 0 2 74 8 80 5.35e-13 64.3
Msa1334320 AT5G49420.1 34.043 141 78 4 3 133 8 143 1.13e-12 65.5
Msa1334320 AT1G60920.1 40.698 86 45 3 3 82 5 90 2.07e-12 63.2
Msa1334320 AT5G04640.1 42.667 75 39 2 3 73 5 79 2.39e-12 64.3
Msa1334320 AT2G03060.2 44.156 77 41 2 1 75 1 77 4.29e-12 63.9
Msa1334320 AT2G03060.3 44.156 77 41 2 1 75 1 77 4.68e-12 63.5
Msa1334320 AT2G03060.7 44.156 77 41 2 1 75 1 77 4.68e-12 63.5
Msa1334320 AT2G03060.1 44.156 77 41 2 1 75 1 77 4.68e-12 63.5
Msa1334320 AT5G49490.1 34.532 139 80 4 3 132 46 182 7.27e-12 62.8
Msa1334320 AT2G26320.1 34.524 84 49 1 1 78 17 100 2.92e-11 58.2
Msa1334320 AT5G38620.1 40.278 72 39 1 3 70 6 77 3.33e-11 61.2
Msa1334320 AT1G77080.5 66.667 39 13 0 1 39 1 39 5.69e-11 58.9
Msa1334320 AT1G77080.11 66.667 39 13 0 1 39 1 39 6.41e-11 58.5

Find 36 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGAATCGACCTTACTCTTT+TGG 0.284342 8_4:-54688734 Msa1334320:CDS
CCTTGGGAATCACTTGGTTC+AGG 0.292476 8_4:+54688665 None:intergenic
TGAAGTGTTGGCACAAATTC+GGG 0.328856 8_4:-54688591 Msa1334320:CDS
ATATAAAGAGCTTCACGATT+TGG 0.333750 8_4:-54688480 Msa1334320:CDS
ATGAAGTGTTGGCACAAATT+CGG 0.337355 8_4:-54688592 Msa1334320:CDS
CATCAGAGGCGGGGTTCCCT+TGG 0.347497 8_4:+54688648 None:intergenic
CACTTGGTTCAGGCTCTTGT+TGG 0.351803 8_4:+54688675 None:intergenic
GAAATTACGTCTTAGTGATA+TGG 0.374504 8_4:-54688456 Msa1334320:CDS
GATCTCTGCTCCGCACAAAA+TGG 0.391172 8_4:+54688778 None:intergenic
GCAGCAACAACATCAATTCC+TGG 0.405755 8_4:+54688707 None:intergenic
AGGCTCTTGTTGGAGATACT+TGG 0.417524 8_4:+54688685 None:intergenic
CTTTCTTGAATAAACCTGTT+CGG 0.422634 8_4:+54688813 None:intergenic
ACATTCTCAAAACGCCGAAC+AGG 0.455680 8_4:-54688827 Msa1334320:CDS
CTTGCTTCAGAATCTCATCA+TGG 0.463297 8_4:+54688546 None:intergenic
ATCAGAGGCGGGGTTCCCTT+GGG 0.466571 8_4:+54688649 None:intergenic
CAGAATCTCATCATGGGTCT+CGG 0.474100 8_4:+54688553 None:intergenic
AATGTGACCTGCCTCGTGTT+GGG 0.485158 8_4:+54688845 None:intergenic
ATATTGGGTGCATCAGAGGC+GGG 0.488127 8_4:+54688638 None:intergenic
TTCTATATTGGGTGCATCAG+AGG 0.498428 8_4:+54688634 None:intergenic
ATCTCTCAATTCTTTAAGCT+CGG 0.500207 8_4:+54688505 None:intergenic
AAAGAGTAAGGTCGATTCCC+AGG 0.524951 8_4:+54688737 None:intergenic
TTGCTTCAGAATCTCATCAT+GGG 0.526253 8_4:+54688547 None:intergenic
GAAAGATCCCAACACGAGGC+AGG 0.532566 8_4:-54688852 Msa1334320:CDS
CCTGGGTGACCAAAAGAGTA+AGG 0.535714 8_4:+54688725 None:intergenic
TATATTGGGTGCATCAGAGG+CGG 0.542480 8_4:+54688637 None:intergenic
GGGTTCCCTTGGGAATCACT+TGG 0.548832 8_4:+54688659 None:intergenic
GAATGTGACCTGCCTCGTGT+TGG 0.574867 8_4:+54688844 None:intergenic
CAGCAACAACATCAATTCCT+GGG 0.584319 8_4:+54688708 None:intergenic
TTTAGCTCAGGATCCAGTGG+TGG 0.589768 8_4:-54688411 Msa1334320:CDS
TATTGGGTGCATCAGAGGCG+GGG 0.600666 8_4:+54688639 None:intergenic
TTAGCTCAGGATCCAGTGGT+GGG 0.603917 8_4:-54688410 Msa1334320:CDS
TCTAGAACTTGATGAAGTGT+TGG 0.605039 8_4:-54688603 Msa1334320:CDS
CCTGAACCAAGTGATTCCCA+AGG 0.649130 8_4:-54688665 Msa1334320:CDS
GTGTTGGCACAAATTCGGGA+AGG 0.674614 8_4:-54688587 Msa1334320:CDS
CTGAACCAAGTGATTCCCAA+GGG 0.694896 8_4:-54688664 Msa1334320:CDS
TCGTGAAAGATCCCAACACG+AGG 0.703551 8_4:-54688856 Msa1334320:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGATTCAATTTTTCTATATT+GGG + chr8_4:54688631-54688650 None:intergenic 15.0%
!!! TAGATTCAATTTTTCTATAT+TGG + chr8_4:54688632-54688651 None:intergenic 15.0%
CTTTCTTGAATAAACCTGTT+CGG + chr8_4:54688441-54688460 None:intergenic 30.0%
ATCTCTCAATTCTTTAAGCT+CGG + chr8_4:54688749-54688768 None:intergenic 30.0%
ATATAAAGAGCTTCACGATT+TGG - chr8_4:54688771-54688790 Msa1334320:CDS 30.0%
GAAATTACGTCTTAGTGATA+TGG - chr8_4:54688795-54688814 Msa1334320:CDS 30.0%
! AATGAGTTGTCCATTTTGTG+CGG - chr8_4:54688463-54688482 Msa1334320:CDS 35.0%
! CTATTGTTGTGTTTTCTCCT+GGG - chr8_4:54688497-54688516 Msa1334320:CDS 35.0%
! TCTAGAACTTGATGAAGTGT+TGG - chr8_4:54688648-54688667 Msa1334320:CDS 35.0%
!! ATGAAGTGTTGGCACAAATT+CGG - chr8_4:54688659-54688678 Msa1334320:CDS 35.0%
TTGCTTCAGAATCTCATCAT+GGG + chr8_4:54688707-54688726 None:intergenic 35.0%
! TTTTCTTTTATTCCCACCAC+TGG + chr8_4:54688856-54688875 None:intergenic 35.0%
! GCTATTGTTGTGTTTTCTCC+TGG - chr8_4:54688496-54688515 Msa1334320:CDS 40.0%
CAGCAACAACATCAATTCCT+GGG + chr8_4:54688546-54688565 None:intergenic 40.0%
TTCTATATTGGGTGCATCAG+AGG + chr8_4:54688620-54688639 None:intergenic 40.0%
!! TGAAGTGTTGGCACAAATTC+GGG - chr8_4:54688660-54688679 Msa1334320:CDS 40.0%
CTTGCTTCAGAATCTCATCA+TGG + chr8_4:54688708-54688727 None:intergenic 40.0%
!!! ATGCATGATGCTTTTAGCTC+AGG - chr8_4:54688828-54688847 Msa1334320:CDS 40.0%
ACATTCTCAAAACGCCGAAC+AGG - chr8_4:54688424-54688443 Msa1334320:CDS 45.0%
! AAAGAGTAAGGTCGATTCCC+AGG + chr8_4:54688517-54688536 None:intergenic 45.0%
! GGGAATCGACCTTACTCTTT+TGG - chr8_4:54688517-54688536 Msa1334320:CDS 45.0%
GCAGCAACAACATCAATTCC+TGG + chr8_4:54688547-54688566 None:intergenic 45.0%
AGGCTCTTGTTGGAGATACT+TGG + chr8_4:54688569-54688588 None:intergenic 45.0%
CTGAACCAAGTGATTCCCAA+GGG - chr8_4:54688587-54688606 Msa1334320:CDS 45.0%
TATATTGGGTGCATCAGAGG+CGG + chr8_4:54688617-54688636 None:intergenic 45.0%
CAGAATCTCATCATGGGTCT+CGG + chr8_4:54688701-54688720 None:intergenic 45.0%
TCGTGAAAGATCCCAACACG+AGG - chr8_4:54688395-54688414 Msa1334320:CDS 50.0%
AATGTGACCTGCCTCGTGTT+GGG + chr8_4:54688409-54688428 None:intergenic 50.0%
GATCTCTGCTCCGCACAAAA+TGG + chr8_4:54688476-54688495 None:intergenic 50.0%
CCTGGGTGACCAAAAGAGTA+AGG + chr8_4:54688529-54688548 None:intergenic 50.0%
! CCTTACTCTTTTGGTCACCC+AGG - chr8_4:54688526-54688545 Msa1334320:CDS 50.0%
CACTTGGTTCAGGCTCTTGT+TGG + chr8_4:54688579-54688598 None:intergenic 50.0%
CCTTGGGAATCACTTGGTTC+AGG + chr8_4:54688589-54688608 None:intergenic 50.0%
CCTGAACCAAGTGATTCCCA+AGG - chr8_4:54688586-54688605 Msa1334320:CDS 50.0%
ATATTGGGTGCATCAGAGGC+GGG + chr8_4:54688616-54688635 None:intergenic 50.0%
! GTGTTGGCACAAATTCGGGA+AGG - chr8_4:54688664-54688683 Msa1334320:CDS 50.0%
!!! GCTTTTAGCTCAGGATCCAG+TGG - chr8_4:54688837-54688856 Msa1334320:CDS 50.0%
! TTTAGCTCAGGATCCAGTGG+TGG - chr8_4:54688840-54688859 Msa1334320:CDS 50.0%
TTAGCTCAGGATCCAGTGGT+GGG - chr8_4:54688841-54688860 Msa1334320:CDS 50.0%
GAAAGATCCCAACACGAGGC+AGG - chr8_4:54688399-54688418 Msa1334320:CDS 55.0%
GAATGTGACCTGCCTCGTGT+TGG + chr8_4:54688410-54688429 None:intergenic 55.0%
GGGTTCCCTTGGGAATCACT+TGG + chr8_4:54688595-54688614 None:intergenic 55.0%
TATTGGGTGCATCAGAGGCG+GGG + chr8_4:54688615-54688634 None:intergenic 55.0%
ATCAGAGGCGGGGTTCCCTT+GGG + chr8_4:54688605-54688624 None:intergenic 60.0%
CATCAGAGGCGGGGTTCCCT+TGG + chr8_4:54688606-54688625 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 54688367 54688906 54688367 ID=Msa1334320;Name=Msa1334320
chr8_4 mRNA 54688367 54688906 54688367 ID=Msa1334320-mRNA-1;Parent=Msa1334320;Name=Msa1334320-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|-1|0|1|-1|1|1|0|179
chr8_4 exon 54688367 54688906 54688367 ID=Msa1334320-mRNA-1:exon:17002;Parent=Msa1334320-mRNA-1
chr8_4 CDS 54688367 54688906 54688367 ID=Msa1334320-mRNA-1:cds;Parent=Msa1334320-mRNA-1
Gene Sequence

>Msa1334320

ATGGGTCGTCGCAAAATTGAGATTGAGATCGTGAAAGATCCCAACACGAGGCAGGTCACATTCTCAAAACGCCGAACAGGTTTATTCAAGAAAGCAAATGAGTTGTCCATTTTGTGCGGAGCAGAGATCGCTATTGTTGTGTTTTCTCCTGGGAATCGACCTTACTCTTTTGGTCACCCAGGAATTGATGTTGTTGCTGCCAAGTATCTCCAACAAGAGCCTGAACCAAGTGATTCCCAAGGGAACCCCGCCTCTGATGCACCCAATATAGAAAAATTGAATCTAGAACTTGATGAAGTGTTGGCACAAATTCGGGAAGGTGAAAAGCAGACCGAGACCCATGATGAGATTCTGAAGCAAGACAATGTGATGAAACTCTCCGAGCTTAAAGAATTGAGAGATTCATATAAAGAGCTTCACGATTTGGTGAAATTACGTCTTAGTGATATGGAGATATCAGAATGCATGATGCTTTTAGCTCAGGATCCAGTGGTGGGAATAAAAGAAAAATCAGCAAGAAAGAAAAGAAGAAAAAATTAA
Protein sequence

>Msa1334320

MGRRKIEIEIVKDPNTRQVTFSKRRTGLFKKANELSILCGAEIAIVVFSPGNRPYSFGHPGIDVVAAKYLQQEPEPSDSQGNPASDAPNIEKLNLELDEVLAQIREGEKQTETHDEILKQDNVMKLSELKELRDSYKELHDLVKLRLSDMEISECMMLLAQDPVVGIKEKSARKKRRKN*