AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1345740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1345740 MtrunA17_Chr7g0271241 85.878 262 17 8 2 260 137 381 2.32e-136 389
Msa1345740 MtrunA17_Chr1g0193371 40.809 272 106 18 1 259 140 369 5.88e-22 93.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 61 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTCTTTCTTTCTTTCTTT+TGG 0.161386 tig0007478:-54730 None:intergenic
CATTTCCAACGTGAATTATT+TGG 0.202469 tig0007478:+54758 Msa1345740:three_prime_UTR
GGGATTCAAAACTGGGAATT+TGG 0.217605 tig0007478:+54319 Msa1345740:CDS
GAACTGTTGTTGTTGGAATT+TGG 0.247083 tig0007478:-54148 None:intergenic
TTATCCATAGGAATAGAATT+TGG 0.264129 tig0007478:-53983 None:intergenic
ATCTCATTGTCTGTTCTTCC+AGG 0.266646 tig0007478:-53766 None:intergenic
GATGATTTGTGTTTGGAGTT+TGG 0.284873 tig0007478:-54264 None:intergenic
AAATAGATTCTTCACTTCAT+TGG 0.293702 tig0007478:-53945 None:intergenic
TTCTTCTCTCAAGTAGATAA+AGG 0.314654 tig0007478:+54970 Msa1345740:three_prime_UTR
ATACCTTCTGATAATATGAT+TGG 0.321312 tig0007478:+54061 Msa1345740:CDS
TCTGATAATATGATTGGATT+AGG 0.329996 tig0007478:+54067 Msa1345740:CDS
AAGTAGTAGAAGACATTAAA+TGG 0.361317 tig0007478:+54410 Msa1345740:CDS
GAGTGCATGAAGTTCAATTA+TGG 0.367556 tig0007478:-53511 None:intergenic
AAGAAGAAGCTAAAGCAAAA+AGG 0.367784 tig0007478:+53815 Msa1345740:five_prime_UTR
GAGAATGGTTGGTGTGTGTT+TGG 0.373639 tig0007478:-53845 None:intergenic
AAGTTGATGGGATTCAAAAC+TGG 0.391933 tig0007478:+54311 Msa1345740:CDS
TTATGAGGTTAAACAAGAAA+TGG 0.400366 tig0007478:+54528 Msa1345740:CDS
TGAGGTGGATAAATTATCTA+AGG 0.408050 tig0007478:+53445 Msa1345740:five_prime_UTR
TTTGAAGGATGATTTGTGTT+TGG 0.410112 tig0007478:-54271 None:intergenic
TATCTACAATGATAATTATG+AGG 0.411745 tig0007478:+54513 Msa1345740:CDS
TGGGTTAAAATGTCACAAAA+AGG 0.421415 tig0007478:+53366 None:intergenic
TTGTCATTGTTCTCAGAGAA+TGG 0.430460 tig0007478:-53860 None:intergenic
AAATCCAAATTCTATTCCTA+TGG 0.434974 tig0007478:+53979 Msa1345740:CDS
AATGCTCCTCTCTTCAAATC+AGG 0.437135 tig0007478:-53468 None:intergenic
ATATCCTCTGGTGAAGTTGA+TGG 0.440161 tig0007478:+54298 Msa1345740:CDS
GCAAAGTGAACTGTTGTTGT+TGG 0.447084 tig0007478:-54155 None:intergenic
CAGATTGCAGCTCAGTTACC+TGG 0.453409 tig0007478:+53748 Msa1345740:five_prime_UTR
CCAGAGGATATAGAATTTGA+AGG 0.456217 tig0007478:-54286 None:intergenic
TTCATGCACTCCTAGGCAAC+AGG 0.471657 tig0007478:+53523 Msa1345740:five_prime_UTR
GATGATTATCTTGATGATCA+AGG 0.492875 tig0007478:-54387 None:intergenic
TAGCTCATCTGAATATCAAA+AGG 0.495807 tig0007478:+53399 Msa1345740:five_prime_UTR
CATTGTTCTCAGAGAATGGT+TGG 0.496170 tig0007478:-53856 None:intergenic
TATCCTCTGGTGAAGTTGAT+GGG 0.501245 tig0007478:+54299 Msa1345740:CDS
ATTGAACTTCATGCACTCCT+AGG 0.501954 tig0007478:+53516 Msa1345740:five_prime_UTR
CCTTCAAATTCTATATCCTC+TGG 0.506664 tig0007478:+54286 Msa1345740:CDS
ATTATTGGTACCTGTTGCCT+AGG 0.519891 tig0007478:-53533 None:intergenic
TTGTCCTCACTTCTGACCAC+AGG 0.528924 tig0007478:+54792 Msa1345740:three_prime_UTR
GATAATATGATTGGATTAGG+AGG 0.542857 tig0007478:+54070 Msa1345740:CDS
CTAAGGCCTGATTTGAAGAG+AGG 0.548695 tig0007478:+53462 Msa1345740:five_prime_UTR
ATAATTCACGTTGGAAATGT+CGG 0.549261 tig0007478:-54754 None:intergenic
ATCTGAATATTGTCAGTCAT+AGG 0.553088 tig0007478:-53902 None:intergenic
GAATTAGCAGCTTCAAAATG+AGG 0.555614 tig0007478:-54601 None:intergenic
CATTTGAGAGCAAGTAGAAG+TGG 0.556588 tig0007478:-54200 None:intergenic
AGTTGATGGGATTCAAAACT+GGG 0.565732 tig0007478:+54312 Msa1345740:CDS
ATTCAAAACTGGGAATTTGG+TGG 0.568240 tig0007478:+54322 Msa1345740:CDS
TGCTTCATACCAAGTACTAG+TGG 0.570925 tig0007478:+54175 Msa1345740:CDS
GAAGTGGAACCACTAGTACT+TGG 0.579651 tig0007478:-54184 None:intergenic
GGTGGAAGCAAAAGCAGCAA+TGG 0.583904 tig0007478:+54340 Msa1345740:CDS
GGATAATTGCAATTATCCAT+AGG 0.590102 tig0007478:-53995 None:intergenic
TCTGACCAAATAATTCACGT+TGG 0.610612 tig0007478:-54763 None:intergenic
AATCCAATCATATTATCAGA+AGG 0.613198 tig0007478:-54064 None:intergenic
TGTCCTCACTTCTGACCACA+GGG 0.613574 tig0007478:+54793 Msa1345740:three_prime_UTR
GATGCAAAAGCTGAAGATTG+AGG 0.618880 tig0007478:+53427 Msa1345740:five_prime_UTR
CACTTCATTGGATCCCACAG+AGG 0.634128 tig0007478:-53933 None:intergenic
AATCCCATCAACTTCACCAG+AGG 0.648801 tig0007478:-54302 None:intergenic
TTCAATCAAGTAGTAGCACA+TGG 0.651827 tig0007478:+54575 Msa1345740:CDS
ACACCCTGTGGTCAGAAGTG+AGG 0.656402 tig0007478:-54796 None:intergenic
GAACATAGAATGAAACATGG+TGG 0.664282 tig0007478:-54242 None:intergenic
GCAAAAGCTGAAGATTGAGG+TGG 0.675817 tig0007478:+53430 Msa1345740:five_prime_UTR
AATTTACAGCACACACCCTG+TGG 0.707334 tig0007478:-54808 None:intergenic
TTGGAACATAGAATGAAACA+TGG 0.723711 tig0007478:-54245 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACCAATAATTATAATAATTA+AGG + tig0007478:53547-53566 Msa1345740:intron 10.0%
!! TCCTTAATTATTATAATTAT+TGG - tig0007478:53551-53570 None:intergenic 10.0%
!! ACCAATAATTATAATAATTA+AGG + tig0007478:53547-53566 Msa1345740:intron 10.0%
!! TCCTTAATTATTATAATTAT+TGG - tig0007478:53551-53570 None:intergenic 10.0%
!!! ATTTTTGAATTAATTTCAAG+GGG - tig0007478:54019-54038 None:intergenic 15.0%
!!! GATTTTTGAATTAATTTCAA+GGG - tig0007478:54020-54039 None:intergenic 15.0%
!!! TGATTTTTGAATTAATTTCA+AGG - tig0007478:54021-54040 None:intergenic 15.0%
!!! ATTTTTGAATTAATTTCAAG+GGG - tig0007478:54019-54038 None:intergenic 15.0%
!!! GATTTTTGAATTAATTTCAA+GGG - tig0007478:54020-54039 None:intergenic 15.0%
!!! TGATTTTTGAATTAATTTCA+AGG - tig0007478:54021-54040 None:intergenic 15.0%
!! ACTGACTAATATATATACTT+CGG + tig0007478:53700-53719 Msa1345740:intron 20.0%
!! CAATGAGATAAAAAAATCTA+TGG + tig0007478:53780-53799 Msa1345740:five_prime_UTR 20.0%
!!! AATTTTCAACAACTTAACTA+TGG + tig0007478:54103-54122 Msa1345740:CDS 20.0%
!! TATCTACAATGATAATTATG+AGG + tig0007478:54513-54532 Msa1345740:CDS 20.0%
!! ACTGACTAATATATATACTT+CGG + tig0007478:53700-53719 Msa1345740:intron 20.0%
!! CAATGAGATAAAAAAATCTA+TGG + tig0007478:53780-53799 Msa1345740:five_prime_UTR 20.0%
!!! AATTTTCAACAACTTAACTA+TGG + tig0007478:54103-54122 Msa1345740:CDS 20.0%
!! TATCTACAATGATAATTATG+AGG + tig0007478:54513-54532 Msa1345740:CDS 20.0%
!! ATAAGTGTTGCATTTAGATT+TGG + tig0007478:53575-53594 Msa1345740:intron 25.0%
! AAATAGATTCTTCACTTCAT+TGG - tig0007478:53948-53967 None:intergenic 25.0%
! AAATCCAAATTCTATTCCTA+TGG + tig0007478:53979-53998 Msa1345740:CDS 25.0%
! TTATCCATAGGAATAGAATT+TGG - tig0007478:53986-54005 None:intergenic 25.0%
! ATACCTTCTGATAATATGAT+TGG + tig0007478:54061-54080 Msa1345740:CDS 25.0%
! AATCCAATCATATTATCAGA+AGG - tig0007478:54067-54086 None:intergenic 25.0%
! TCTGATAATATGATTGGATT+AGG + tig0007478:54067-54086 Msa1345740:CDS 25.0%
! AAGTAGTAGAAGACATTAAA+TGG + tig0007478:54410-54429 Msa1345740:CDS 25.0%
!!! TTGTTGTTTCCAAGAAAAAA+TGG - tig0007478:54457-54476 None:intergenic 25.0%
! TTATGAGGTTAAACAAGAAA+TGG + tig0007478:54528-54547 Msa1345740:CDS 25.0%
!! ATCTCTTTCTTTCTTTCTTT+TGG - tig0007478:54733-54752 None:intergenic 25.0%
!! ATAAGTGTTGCATTTAGATT+TGG + tig0007478:53575-53594 Msa1345740:intron 25.0%
! AAATAGATTCTTCACTTCAT+TGG - tig0007478:53948-53967 None:intergenic 25.0%
! AAATCCAAATTCTATTCCTA+TGG + tig0007478:53979-53998 Msa1345740:CDS 25.0%
! TTATCCATAGGAATAGAATT+TGG - tig0007478:53986-54005 None:intergenic 25.0%
! ATACCTTCTGATAATATGAT+TGG + tig0007478:54061-54080 Msa1345740:CDS 25.0%
! AATCCAATCATATTATCAGA+AGG - tig0007478:54067-54086 None:intergenic 25.0%
! TCTGATAATATGATTGGATT+AGG + tig0007478:54067-54086 Msa1345740:CDS 25.0%
! AAGTAGTAGAAGACATTAAA+TGG + tig0007478:54410-54429 Msa1345740:CDS 25.0%
!!! TTGTTGTTTCCAAGAAAAAA+TGG - tig0007478:54457-54476 None:intergenic 25.0%
! TTATGAGGTTAAACAAGAAA+TGG + tig0007478:54528-54547 Msa1345740:CDS 25.0%
!! ATCTCTTTCTTTCTTTCTTT+TGG - tig0007478:54733-54752 None:intergenic 25.0%
TAGCTCATCTGAATATCAAA+AGG + tig0007478:53399-53418 Msa1345740:five_prime_UTR 30.0%
TGAGGTGGATAAATTATCTA+AGG + tig0007478:53445-53464 Msa1345740:five_prime_UTR 30.0%
AAGAAGAAGCTAAAGCAAAA+AGG + tig0007478:53815-53834 Msa1345740:five_prime_UTR 30.0%
ATCTGAATATTGTCAGTCAT+AGG - tig0007478:53905-53924 None:intergenic 30.0%
GGATAATTGCAATTATCCAT+AGG - tig0007478:53998-54017 None:intergenic 30.0%
! GATAATATGATTGGATTAGG+AGG + tig0007478:54070-54089 Msa1345740:CDS 30.0%
TTGGAACATAGAATGAAACA+TGG - tig0007478:54248-54267 None:intergenic 30.0%
! TTTGAAGGATGATTTGTGTT+TGG - tig0007478:54274-54293 None:intergenic 30.0%
! GATGATTATCTTGATGATCA+AGG - tig0007478:54390-54409 None:intergenic 30.0%
!!! AACTCTAGTCCATTTTTTCT+TGG + tig0007478:54445-54464 Msa1345740:CDS 30.0%
! AGATCTTCAAAGATTTTCAC+TGG + tig0007478:54642-54661 Msa1345740:CDS 30.0%
ATAATTCACGTTGGAAATGT+CGG - tig0007478:54757-54776 None:intergenic 30.0%
CATTTCCAACGTGAATTATT+TGG + tig0007478:54758-54777 Msa1345740:three_prime_UTR 30.0%
TTCTTCTCTCAAGTAGATAA+AGG + tig0007478:54970-54989 Msa1345740:three_prime_UTR 30.0%
TAGCTCATCTGAATATCAAA+AGG + tig0007478:53399-53418 Msa1345740:five_prime_UTR 30.0%
TGAGGTGGATAAATTATCTA+AGG + tig0007478:53445-53464 Msa1345740:five_prime_UTR 30.0%
AAGAAGAAGCTAAAGCAAAA+AGG + tig0007478:53815-53834 Msa1345740:five_prime_UTR 30.0%
ATCTGAATATTGTCAGTCAT+AGG - tig0007478:53905-53924 None:intergenic 30.0%
GGATAATTGCAATTATCCAT+AGG - tig0007478:53998-54017 None:intergenic 30.0%
! GATAATATGATTGGATTAGG+AGG + tig0007478:54070-54089 Msa1345740:CDS 30.0%
TTGGAACATAGAATGAAACA+TGG - tig0007478:54248-54267 None:intergenic 30.0%
! TTTGAAGGATGATTTGTGTT+TGG - tig0007478:54274-54293 None:intergenic 30.0%
! GATGATTATCTTGATGATCA+AGG - tig0007478:54390-54409 None:intergenic 30.0%
!!! AACTCTAGTCCATTTTTTCT+TGG + tig0007478:54445-54464 Msa1345740:CDS 30.0%
! AGATCTTCAAAGATTTTCAC+TGG + tig0007478:54642-54661 Msa1345740:CDS 30.0%
ATAATTCACGTTGGAAATGT+CGG - tig0007478:54757-54776 None:intergenic 30.0%
CATTTCCAACGTGAATTATT+TGG + tig0007478:54758-54777 Msa1345740:three_prime_UTR 30.0%
TTCTTCTCTCAAGTAGATAA+AGG + tig0007478:54970-54989 Msa1345740:three_prime_UTR 30.0%
GAGTGCATGAAGTTCAATTA+TGG - tig0007478:53514-53533 None:intergenic 35.0%
!! TCGGTGCTTTTTGAATTATC+AGG + tig0007478:53719-53738 Msa1345740:intron 35.0%
!! GTGCTTTTTGAATTATCAGG+TGG + tig0007478:53722-53741 Msa1345740:intron 35.0%
TTGTCATTGTTCTCAGAGAA+TGG - tig0007478:53863-53882 None:intergenic 35.0%
TCAGATTCAAAAAACCTCTG+TGG + tig0007478:53919-53938 Msa1345740:CDS 35.0%
CAGATTCAAAAAACCTCTGT+GGG + tig0007478:53920-53939 Msa1345740:CDS 35.0%
! GAACTGTTGTTGTTGGAATT+TGG - tig0007478:54151-54170 None:intergenic 35.0%
GAACATAGAATGAAACATGG+TGG - tig0007478:54245-54264 None:intergenic 35.0%
! GATGATTTGTGTTTGGAGTT+TGG - tig0007478:54267-54286 None:intergenic 35.0%
CCAGAGGATATAGAATTTGA+AGG - tig0007478:54289-54308 None:intergenic 35.0%
CCTTCAAATTCTATATCCTC+TGG + tig0007478:54286-54305 Msa1345740:CDS 35.0%
!! AAGTTGATGGGATTCAAAAC+TGG + tig0007478:54311-54330 Msa1345740:CDS 35.0%
!! AGTTGATGGGATTCAAAACT+GGG + tig0007478:54312-54331 Msa1345740:CDS 35.0%
ATTCAAAACTGGGAATTTGG+TGG + tig0007478:54322-54341 Msa1345740:CDS 35.0%
TTCAATCAAGTAGTAGCACA+TGG + tig0007478:54575-54594 Msa1345740:CDS 35.0%
GAATTAGCAGCTTCAAAATG+AGG - tig0007478:54604-54623 None:intergenic 35.0%
!!! CAAAGATTTTCACTGGCTTT+TGG + tig0007478:54649-54668 Msa1345740:CDS 35.0%
! ATTTTCACTTTACACGTACG+TGG + tig0007478:54694-54713 Msa1345740:three_prime_UTR 35.0%
TCTGACCAAATAATTCACGT+TGG - tig0007478:54766-54785 None:intergenic 35.0%
GAGTGCATGAAGTTCAATTA+TGG - tig0007478:53514-53533 None:intergenic 35.0%
!! TCGGTGCTTTTTGAATTATC+AGG + tig0007478:53719-53738 Msa1345740:intron 35.0%
!! GTGCTTTTTGAATTATCAGG+TGG + tig0007478:53722-53741 Msa1345740:intron 35.0%
TTGTCATTGTTCTCAGAGAA+TGG - tig0007478:53863-53882 None:intergenic 35.0%
TCAGATTCAAAAAACCTCTG+TGG + tig0007478:53919-53938 Msa1345740:CDS 35.0%
CAGATTCAAAAAACCTCTGT+GGG + tig0007478:53920-53939 Msa1345740:CDS 35.0%
! GAACTGTTGTTGTTGGAATT+TGG - tig0007478:54151-54170 None:intergenic 35.0%
GAACATAGAATGAAACATGG+TGG - tig0007478:54245-54264 None:intergenic 35.0%
! GATGATTTGTGTTTGGAGTT+TGG - tig0007478:54267-54286 None:intergenic 35.0%
CCAGAGGATATAGAATTTGA+AGG - tig0007478:54289-54308 None:intergenic 35.0%
CCTTCAAATTCTATATCCTC+TGG + tig0007478:54286-54305 Msa1345740:CDS 35.0%
!! AAGTTGATGGGATTCAAAAC+TGG + tig0007478:54311-54330 Msa1345740:CDS 35.0%
!! AGTTGATGGGATTCAAAACT+GGG + tig0007478:54312-54331 Msa1345740:CDS 35.0%
ATTCAAAACTGGGAATTTGG+TGG + tig0007478:54322-54341 Msa1345740:CDS 35.0%
TTCAATCAAGTAGTAGCACA+TGG + tig0007478:54575-54594 Msa1345740:CDS 35.0%
GAATTAGCAGCTTCAAAATG+AGG - tig0007478:54604-54623 None:intergenic 35.0%
!!! CAAAGATTTTCACTGGCTTT+TGG + tig0007478:54649-54668 Msa1345740:CDS 35.0%
! ATTTTCACTTTACACGTACG+TGG + tig0007478:54694-54713 Msa1345740:three_prime_UTR 35.0%
TCTGACCAAATAATTCACGT+TGG - tig0007478:54766-54785 None:intergenic 35.0%
GATGCAAAAGCTGAAGATTG+AGG + tig0007478:53427-53446 Msa1345740:five_prime_UTR 40.0%
AATGCTCCTCTCTTCAAATC+AGG - tig0007478:53471-53490 None:intergenic 40.0%
ATTGAACTTCATGCACTCCT+AGG + tig0007478:53516-53535 Msa1345740:five_prime_UTR 40.0%
ATTATTGGTACCTGTTGCCT+AGG - tig0007478:53536-53555 None:intergenic 40.0%
ATCTCATTGTCTGTTCTTCC+AGG - tig0007478:53769-53788 None:intergenic 40.0%
CATTGTTCTCAGAGAATGGT+TGG - tig0007478:53859-53878 None:intergenic 40.0%
GCAAAGTGAACTGTTGTTGT+TGG - tig0007478:54158-54177 None:intergenic 40.0%
TGCTTCATACCAAGTACTAG+TGG + tig0007478:54175-54194 Msa1345740:CDS 40.0%
CATTTGAGAGCAAGTAGAAG+TGG - tig0007478:54203-54222 None:intergenic 40.0%
ATATCCTCTGGTGAAGTTGA+TGG + tig0007478:54298-54317 Msa1345740:CDS 40.0%
! TATCCTCTGGTGAAGTTGAT+GGG + tig0007478:54299-54318 Msa1345740:CDS 40.0%
GGGATTCAAAACTGGGAATT+TGG + tig0007478:54319-54338 Msa1345740:CDS 40.0%
GATGCAAAAGCTGAAGATTG+AGG + tig0007478:53427-53446 Msa1345740:five_prime_UTR 40.0%
AATGCTCCTCTCTTCAAATC+AGG - tig0007478:53471-53490 None:intergenic 40.0%
ATTGAACTTCATGCACTCCT+AGG + tig0007478:53516-53535 Msa1345740:five_prime_UTR 40.0%
ATTATTGGTACCTGTTGCCT+AGG - tig0007478:53536-53555 None:intergenic 40.0%
ATCTCATTGTCTGTTCTTCC+AGG - tig0007478:53769-53788 None:intergenic 40.0%
CATTGTTCTCAGAGAATGGT+TGG - tig0007478:53859-53878 None:intergenic 40.0%
GCAAAGTGAACTGTTGTTGT+TGG - tig0007478:54158-54177 None:intergenic 40.0%
TGCTTCATACCAAGTACTAG+TGG + tig0007478:54175-54194 Msa1345740:CDS 40.0%
CATTTGAGAGCAAGTAGAAG+TGG - tig0007478:54203-54222 None:intergenic 40.0%
ATATCCTCTGGTGAAGTTGA+TGG + tig0007478:54298-54317 Msa1345740:CDS 40.0%
! TATCCTCTGGTGAAGTTGAT+GGG + tig0007478:54299-54318 Msa1345740:CDS 40.0%
GGGATTCAAAACTGGGAATT+TGG + tig0007478:54319-54338 Msa1345740:CDS 40.0%
GCAAAAGCTGAAGATTGAGG+TGG + tig0007478:53430-53449 Msa1345740:five_prime_UTR 45.0%
! CTAAGGCCTGATTTGAAGAG+AGG + tig0007478:53462-53481 Msa1345740:five_prime_UTR 45.0%
! GAGAATGGTTGGTGTGTGTT+TGG - tig0007478:53848-53867 None:intergenic 45.0%
GAAGTGGAACCACTAGTACT+TGG - tig0007478:54187-54206 None:intergenic 45.0%
AATCCCATCAACTTCACCAG+AGG - tig0007478:54305-54324 None:intergenic 45.0%
AATTTACAGCACACACCCTG+TGG - tig0007478:54811-54830 None:intergenic 45.0%
GCAAAAGCTGAAGATTGAGG+TGG + tig0007478:53430-53449 Msa1345740:five_prime_UTR 45.0%
! CTAAGGCCTGATTTGAAGAG+AGG + tig0007478:53462-53481 Msa1345740:five_prime_UTR 45.0%
! GAGAATGGTTGGTGTGTGTT+TGG - tig0007478:53848-53867 None:intergenic 45.0%
GAAGTGGAACCACTAGTACT+TGG - tig0007478:54187-54206 None:intergenic 45.0%
AATCCCATCAACTTCACCAG+AGG - tig0007478:54305-54324 None:intergenic 45.0%
AATTTACAGCACACACCCTG+TGG - tig0007478:54811-54830 None:intergenic 45.0%
TTCATGCACTCCTAGGCAAC+AGG + tig0007478:53523-53542 Msa1345740:five_prime_UTR 50.0%
CAGATTGCAGCTCAGTTACC+TGG + tig0007478:53748-53767 Msa1345740:five_prime_UTR 50.0%
CACTTCATTGGATCCCACAG+AGG - tig0007478:53936-53955 None:intergenic 50.0%
GGTGGAAGCAAAAGCAGCAA+TGG + tig0007478:54340-54359 Msa1345740:CDS 50.0%
TTGTCCTCACTTCTGACCAC+AGG + tig0007478:54792-54811 Msa1345740:three_prime_UTR 50.0%
TGTCCTCACTTCTGACCACA+GGG + tig0007478:54793-54812 Msa1345740:three_prime_UTR 50.0%
TTCATGCACTCCTAGGCAAC+AGG + tig0007478:53523-53542 Msa1345740:five_prime_UTR 50.0%
CAGATTGCAGCTCAGTTACC+TGG + tig0007478:53748-53767 Msa1345740:five_prime_UTR 50.0%
CACTTCATTGGATCCCACAG+AGG - tig0007478:53936-53955 None:intergenic 50.0%
GGTGGAAGCAAAAGCAGCAA+TGG + tig0007478:54340-54359 Msa1345740:CDS 50.0%
TTGTCCTCACTTCTGACCAC+AGG + tig0007478:54792-54811 Msa1345740:three_prime_UTR 50.0%
TGTCCTCACTTCTGACCACA+GGG + tig0007478:54793-54812 Msa1345740:three_prime_UTR 50.0%
ACACCCTGTGGTCAGAAGTG+AGG - tig0007478:54799-54818 None:intergenic 55.0%
ACACCCTGTGGTCAGAAGTG+AGG - tig0007478:54799-54818 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
tig0007478 gene 53375 54995 53375 ID=Msa1345740;Name=Msa1345740
tig0007478 mRNA 53375 54995 53375 ID=Msa1345740-mRNA-1;Parent=Msa1345740;Name=Msa1345740-mRNA-1;_AED=0.00;_eAED=0.00;_QI=334|1|1|1|1|1|2|308|260
tig0007478 exon 53375 53544 53375 ID=Msa1345740-mRNA-1:exon:5133;Parent=Msa1345740-mRNA-1
tig0007478 exon 53741 54995 53741 ID=Msa1345740-mRNA-1:exon:5134;Parent=Msa1345740-mRNA-1
tig0007478 five_prime_UTR 53375 53544 53375 ID=Msa1345740-mRNA-1:five_prime_utr;Parent=Msa1345740-mRNA-1
tig0007478 five_prime_UTR 53741 53904 53741 ID=Msa1345740-mRNA-1:five_prime_utr;Parent=Msa1345740-mRNA-1
tig0007478 CDS 53905 54687 53905 ID=Msa1345740-mRNA-1:cds;Parent=Msa1345740-mRNA-1
tig0007478 three_prime_UTR 54688 54995 54688 ID=Msa1345740-mRNA-1:three_prime_utr;Parent=Msa1345740-mRNA-1
Gene Sequence

>Msa1345740

ATGACTGACAATATTCAGATTCAAAAAACCTCTGTGGGATCCAATGAAGTGAAGAATCTATTTGATGATACACTAAATCCAAATTCTATTCCTATGGATAATTGCAATTATCCCCTTGAAATTAATTCAAAAATCAACAACTCAGTTAATTGCTTCATACCTTCTGATAATATGATTGGATTAGGAGGATCATATCTCAATTTTCAACAACTTAACTATGGAATTAATAACATGACTCTATCTCCAAATTCCAACAACAACAGTTCACTTTGCTTCATACCAAGTACTAGTGGTTCCACTTCTACTTGCTCTCAAATGATGTCAGAATTCAATTCTTCCACCATGTTTCATTCTATGTTCCAAACTCCAAACACAAATCATCCTTCAAATTCTATATCCTCTGGTGAAGTTGATGGGATTCAAAACTGGGAATTTGGTGGAAGCAAAAGCAGCAATGGAAGCAGCAGTAGCAACATTAATTACCTTGATCATCAAGATAATCATCAAGTAGTAGAAGACATTAAATGGTCTGATTATATAAACTCTAGTCCATTTTTTCTTGGAAACAACAACACATTTCAGCAGCAGAATCATCAAACACACTCTTCTATCTACAATGATAATTATGAGGTTAAACAAGAAATGGAGTTCATTAATACAAACGAATCTATTCAATCAAGTAGTAGCACATGGCATCCTCATTTTGAAGCTGCTAATTCAGATATATATAGCAACAAAGATCTTCAAAGATTTTCACTGGCTTTTGGACAAACACTTAATTAG
Protein sequence

>Msa1345740

MTDNIQIQKTSVGSNEVKNLFDDTLNPNSIPMDNCNYPLEINSKINNSVNCFIPSDNMIGLGGSYLNFQQLNYGINNMTLSPNSNNNSSLCFIPSTSGSTSTCSQMMSEFNSSTMFHSMFQTPNTNHPSNSISSGEVDGIQNWEFGGSKSSNGSSSSNINYLDHQDNHQVVEDIKWSDYINSSPFFLGNNNTFQQQNHQTHSSIYNDNYEVKQEMEFINTNESIQSSSSTWHPHFEAANSDIYSNKDLQRFSLAFGQTLN*