AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1359200


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1359200 MtrunA17_Chr4g0054501 85.526 76 11 0 38 113 12 87 3.30e-43 136
Msa1359200 MtrunA17_Chr1g0158721 75.806 62 12 1 39 97 13 74 1.83e-25 94.0
Msa1359200 MtrunA17_Chr6g0449691 78.689 61 11 1 25 85 181 239 1.73e-24 96.3
Msa1359200 MtrunA17_Chr3g0113101 55.294 85 26 1 1 85 97 169 2.16e-21 87.0
Msa1359200 MtrunA17_Chr6g0487231 69.231 39 12 0 38 76 175 213 1.29e-12 62.4
Msa1359200 MtrunA17_Chr4g0070851 56.818 44 19 0 39 82 177 220 1.90e-11 59.3
Msa1359200 MtrunA17_Chr2g0299991 51.111 45 22 0 38 82 176 220 4.38e-11 58.2
Msa1359200 MtrunA17_Chr5g0410861 62.791 43 15 1 38 80 29 70 6.56e-11 54.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1359200 AT5G40440.4 87.234 47 6 0 39 85 192 238 3.59e-23 93.2
Msa1359200 AT5G40440.1 87.234 47 6 0 39 85 192 238 3.59e-23 93.2
Msa1359200 AT5G40440.5 87.234 47 6 0 39 85 110 156 3.67e-23 92.8
Msa1359200 AT5G40440.6 87.234 47 6 0 39 85 174 220 4.46e-23 92.8
Msa1359200 AT5G40440.3 87.234 47 6 0 39 85 174 220 4.46e-23 92.8
Msa1359200 AT5G40440.2 87.234 47 6 0 39 85 174 220 4.46e-23 92.8
Msa1359200 AT3G55800.1 49.412 85 31 1 1 85 104 176 1.32e-18 79.7
Msa1359200 AT5G56580.1 60.000 45 18 0 40 84 179 223 1.45e-12 62.8
Msa1359200 AT4G29810.3 53.191 47 22 0 39 85 186 232 5.42e-12 61.2
Msa1359200 AT4G29810.1 53.191 47 22 0 39 85 177 223 5.92e-12 61.2
Msa1359200 AT4G29810.2 53.191 47 22 0 39 85 186 232 6.17e-12 60.8

Find 14 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACAATGTGTTAACTCTTT+TGG 0.201562 tig0016663:+3610 Msa1359200:CDS
GAGCCGAAAATTACTGATTT+TGG 0.210770 tig0016663:+4634 Msa1359200:CDS
GCTCAACAAGATCCTCAATA+AGG 0.411586 tig0016663:-4919 Msa1359200:intergenic
ATTTACTTGTAAACCTCAAA+GGG 0.420841 tig0016663:+4611 Msa1359200:CDS
AGGTTTACAAGTAAATTAGC+AGG 0.487423 tig0016663:-4604 Msa1359200:intergenic
AATTTACTTGTAAACCTCAA+AGG 0.501450 tig0016663:+4610 Msa1359200:CDS
CATAGAGAATTCAGTTGCAA+TGG 0.563989 tig0016663:+4669 Msa1359200:CDS
AGATGAGCAAGTCCTTATTG+AGG 0.590575 tig0016663:+4907 Msa1359200:CDS
TGGGGATGAGCAGCTTGCAG+TGG 0.595661 tig0016663:+3630 Msa1359200:CDS
GCAGCTTGCAGTGGATATGA+TGG 0.623243 tig0016663:+3639 Msa1359200:CDS
GTGAAGACAACTTCTTGTGG+TGG 0.628890 tig0016663:+3586 Msa1359200:CDS
AAAGTGAAGACAACTTCTTG+TGG 0.638471 tig0016663:+3583 Msa1359200:CDS
AAGGGTCTAAGTTATCTGCA+CGG 0.655190 tig0016663:+4550 Msa1359200:intron
TTTACTTGTAAACCTCAAAG+GGG 0.694553 tig0016663:+4612 Msa1359200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTTTTAATCTTTTTGAA+GGG + tig0016663:4532-4551 Msa1359200:intron 15.0%
!!! GTTTTTTTAATCTTTTTGAA+GGG + tig0016663:4532-4551 Msa1359200:intron 15.0%
!! TAATTAAACTGATTATGTAG+TGG + tig0016663:3751-3770 Msa1359200:intron 20.0%
!!! TCATAATTTGTTGTTTCATT+AGG - tig0016663:3796-3815 Msa1359200:intergenic 20.0%
!! AAATTAAACAATACAACATG+TGG + tig0016663:3932-3951 Msa1359200:intron 20.0%
!! ACTCTATTAGTGATATAATT+AGG + tig0016663:4229-4248 Msa1359200:intron 20.0%
!! AACATGAAACAATTATGATT+TGG - tig0016663:4304-4323 Msa1359200:intergenic 20.0%
!!! GGTTTTTTTAATCTTTTTGA+AGG + tig0016663:4531-4550 Msa1359200:intron 20.0%
!!! ATACCAAAATCAGTAATTTT+CGG - tig0016663:4640-4659 Msa1359200:intergenic 20.0%
!! TAATTAAACTGATTATGTAG+TGG + tig0016663:3751-3770 Msa1359200:intron 20.0%
!!! TCATAATTTGTTGTTTCATT+AGG - tig0016663:3796-3815 Msa1359200:intergenic 20.0%
!! AAATTAAACAATACAACATG+TGG + tig0016663:3932-3951 Msa1359200:intron 20.0%
!! ACTCTATTAGTGATATAATT+AGG + tig0016663:4229-4248 Msa1359200:intron 20.0%
!! AACATGAAACAATTATGATT+TGG - tig0016663:4304-4323 Msa1359200:intergenic 20.0%
!!! GGTTTTTTTAATCTTTTTGA+AGG + tig0016663:4531-4550 Msa1359200:intron 20.0%
!!! ATACCAAAATCAGTAATTTT+CGG - tig0016663:4640-4659 Msa1359200:intergenic 20.0%
!!! ACAAAAGTATCAGAATCTTA+CGG - tig0016663:3856-3875 Msa1359200:intergenic 25.0%
!!! TTGATTTGTGTTCATATAAC+AGG + tig0016663:4004-4023 Msa1359200:intron 25.0%
! AATAGAGTCACACAAATAAA+GGG - tig0016663:4217-4236 Msa1359200:intergenic 25.0%
! TAATAGAGTCACACAAATAA+AGG - tig0016663:4218-4237 Msa1359200:intergenic 25.0%
! ATCATTTCCACACAAATAAA+GGG - tig0016663:4263-4282 Msa1359200:intergenic 25.0%
! ATGATCTCAAACATTTAAGA+AGG + tig0016663:4278-4297 Msa1359200:intron 25.0%
! TGAAACATGGATGATAAAAT+AGG - tig0016663:4416-4435 Msa1359200:intergenic 25.0%
! AATTTACTTGTAAACCTCAA+AGG + tig0016663:4610-4629 Msa1359200:CDS 25.0%
! ATTTACTTGTAAACCTCAAA+GGG + tig0016663:4611-4630 Msa1359200:CDS 25.0%
!!! ACAAAAGTATCAGAATCTTA+CGG - tig0016663:3856-3875 Msa1359200:intergenic 25.0%
!!! TTGATTTGTGTTCATATAAC+AGG + tig0016663:4004-4023 Msa1359200:intron 25.0%
! AATAGAGTCACACAAATAAA+GGG - tig0016663:4217-4236 Msa1359200:intergenic 25.0%
! TAATAGAGTCACACAAATAA+AGG - tig0016663:4218-4237 Msa1359200:intergenic 25.0%
! ATCATTTCCACACAAATAAA+GGG - tig0016663:4263-4282 Msa1359200:intergenic 25.0%
! ATGATCTCAAACATTTAAGA+AGG + tig0016663:4278-4297 Msa1359200:intron 25.0%
! TGAAACATGGATGATAAAAT+AGG - tig0016663:4416-4435 Msa1359200:intergenic 25.0%
! AATTTACTTGTAAACCTCAA+AGG + tig0016663:4610-4629 Msa1359200:CDS 25.0%
! ATTTACTTGTAAACCTCAAA+GGG + tig0016663:4611-4630 Msa1359200:CDS 25.0%
! ACACAATGTGTTAACTCTTT+TGG + tig0016663:3610-3629 Msa1359200:CDS 30.0%
! CACAATGTGTTAACTCTTTT+GGG + tig0016663:3611-3630 Msa1359200:CDS 30.0%
! ACAATGTGTTAACTCTTTTG+GGG + tig0016663:3612-3631 Msa1359200:CDS 30.0%
! TACCATGCAATAGTAGTTTT+AGG + tig0016663:4081-4100 Msa1359200:intron 30.0%
! ACTTATTCATGATAGCGTTA+AGG + tig0016663:4121-4140 Msa1359200:intron 30.0%
GATCATTTCCACACAAATAA+AGG - tig0016663:4264-4283 Msa1359200:intergenic 30.0%
! AATTGTTTCATGTTGACTGA+TGG + tig0016663:4310-4329 Msa1359200:intron 30.0%
ATGGATGATAAAATAGGTTC+GGG - tig0016663:4410-4429 Msa1359200:intergenic 30.0%
CATGGATGATAAAATAGGTT+CGG - tig0016663:4411-4430 Msa1359200:intergenic 30.0%
ATTGAGATATGCTACTAACT+TGG + tig0016663:4506-4525 Msa1359200:intron 30.0%
AGGTTTACAAGTAAATTAGC+AGG - tig0016663:4607-4626 Msa1359200:intergenic 30.0%
TTTACTTGTAAACCTCAAAG+GGG + tig0016663:4612-4631 Msa1359200:CDS 30.0%
!! ACTGATTTTGGTATTAGTGT+TGG + tig0016663:4646-4665 Msa1359200:CDS 30.0%
GAAGTGCAATTTCTATCTAT+AGG - tig0016663:4884-4903 Msa1359200:intergenic 30.0%
! ACACAATGTGTTAACTCTTT+TGG + tig0016663:3610-3629 Msa1359200:CDS 30.0%
! CACAATGTGTTAACTCTTTT+GGG + tig0016663:3611-3630 Msa1359200:CDS 30.0%
! ACAATGTGTTAACTCTTTTG+GGG + tig0016663:3612-3631 Msa1359200:CDS 30.0%
! TACCATGCAATAGTAGTTTT+AGG + tig0016663:4081-4100 Msa1359200:intron 30.0%
! ACTTATTCATGATAGCGTTA+AGG + tig0016663:4121-4140 Msa1359200:intron 30.0%
GATCATTTCCACACAAATAA+AGG - tig0016663:4264-4283 Msa1359200:intergenic 30.0%
! AATTGTTTCATGTTGACTGA+TGG + tig0016663:4310-4329 Msa1359200:intron 30.0%
ATGGATGATAAAATAGGTTC+GGG - tig0016663:4410-4429 Msa1359200:intergenic 30.0%
CATGGATGATAAAATAGGTT+CGG - tig0016663:4411-4430 Msa1359200:intergenic 30.0%
ATTGAGATATGCTACTAACT+TGG + tig0016663:4506-4525 Msa1359200:intron 30.0%
AGGTTTACAAGTAAATTAGC+AGG - tig0016663:4607-4626 Msa1359200:intergenic 30.0%
TTTACTTGTAAACCTCAAAG+GGG + tig0016663:4612-4631 Msa1359200:CDS 30.0%
!! ACTGATTTTGGTATTAGTGT+TGG + tig0016663:4646-4665 Msa1359200:CDS 30.0%
GAAGTGCAATTTCTATCTAT+AGG - tig0016663:4884-4903 Msa1359200:intergenic 30.0%
AAAGTGAAGACAACTTCTTG+TGG + tig0016663:3583-3602 Msa1359200:CDS 35.0%
! GGCTAATAATCTTCGTTTTG+AGG + tig0016663:3660-3679 Msa1359200:CDS 35.0%
CATCAATAACTCATCTGCTT+CGG + tig0016663:3824-3843 Msa1359200:intron 35.0%
ATCAATAACTCATCTGCTTC+GGG + tig0016663:3825-3844 Msa1359200:intron 35.0%
GTCCTAAAACTACTATTGCA+TGG - tig0016663:4086-4105 Msa1359200:intergenic 35.0%
! GTGATACTTTTGTGGCAATT+AGG + tig0016663:4332-4351 Msa1359200:intron 35.0%
TTTGTGGCAATTAGGAGATA+AGG + tig0016663:4340-4359 Msa1359200:intron 35.0%
AATATATGCTCCTTACACGT+AGG - tig0016663:4445-4464 Msa1359200:intergenic 35.0%
AGATATGCTACTAACTTGGT+TGG + tig0016663:4510-4529 Msa1359200:intron 35.0%
!!! GAGCCGAAAATTACTGATTT+TGG + tig0016663:4634-4653 Msa1359200:CDS 35.0%
CATAGAGAATTCAGTTGCAA+TGG + tig0016663:4669-4688 Msa1359200:CDS 35.0%
AACAAAGTGTGGAAGCAAAT+CGG - tig0016663:4735-4754 Msa1359200:intergenic 35.0%
AAAGTGAAGACAACTTCTTG+TGG + tig0016663:3583-3602 Msa1359200:CDS 35.0%
! GGCTAATAATCTTCGTTTTG+AGG + tig0016663:3660-3679 Msa1359200:CDS 35.0%
CATCAATAACTCATCTGCTT+CGG + tig0016663:3824-3843 Msa1359200:intron 35.0%
ATCAATAACTCATCTGCTTC+GGG + tig0016663:3825-3844 Msa1359200:intron 35.0%
GTCCTAAAACTACTATTGCA+TGG - tig0016663:4086-4105 Msa1359200:intergenic 35.0%
! GTGATACTTTTGTGGCAATT+AGG + tig0016663:4332-4351 Msa1359200:intron 35.0%
TTTGTGGCAATTAGGAGATA+AGG + tig0016663:4340-4359 Msa1359200:intron 35.0%
AATATATGCTCCTTACACGT+AGG - tig0016663:4445-4464 Msa1359200:intergenic 35.0%
AGATATGCTACTAACTTGGT+TGG + tig0016663:4510-4529 Msa1359200:intron 35.0%
!!! GAGCCGAAAATTACTGATTT+TGG + tig0016663:4634-4653 Msa1359200:CDS 35.0%
CATAGAGAATTCAGTTGCAA+TGG + tig0016663:4669-4688 Msa1359200:CDS 35.0%
AACAAAGTGTGGAAGCAAAT+CGG - tig0016663:4735-4754 Msa1359200:intergenic 35.0%
TTCCACACAAATAAAGGGTC+TGG - tig0016663:4258-4277 Msa1359200:intergenic 40.0%
!! GACTGATGGTGATACTTTTG+TGG + tig0016663:4324-4343 Msa1359200:intron 40.0%
GGAGATAAGGACATTATGTG+AGG + tig0016663:4353-4372 Msa1359200:intron 40.0%
TCTACAAGACCCTCATAACA+AGG - tig0016663:4381-4400 Msa1359200:intergenic 40.0%
AAGGGTCTAAGTTATCTGCA+CGG + tig0016663:4550-4569 Msa1359200:intron 40.0%
TCAGTCAAGCAAACAAAGTG+TGG - tig0016663:4746-4765 Msa1359200:intergenic 40.0%
AGATGAGCAAGTCCTTATTG+AGG + tig0016663:4907-4926 Msa1359200:CDS 40.0%
GCTCAACAAGATCCTCAATA+AGG - tig0016663:4922-4941 Msa1359200:intergenic 40.0%
TTCCACACAAATAAAGGGTC+TGG - tig0016663:4258-4277 Msa1359200:intergenic 40.0%
!! GACTGATGGTGATACTTTTG+TGG + tig0016663:4324-4343 Msa1359200:intron 40.0%
GGAGATAAGGACATTATGTG+AGG + tig0016663:4353-4372 Msa1359200:intron 40.0%
TCTACAAGACCCTCATAACA+AGG - tig0016663:4381-4400 Msa1359200:intergenic 40.0%
AAGGGTCTAAGTTATCTGCA+CGG + tig0016663:4550-4569 Msa1359200:intron 40.0%
TCAGTCAAGCAAACAAAGTG+TGG - tig0016663:4746-4765 Msa1359200:intergenic 40.0%
AGATGAGCAAGTCCTTATTG+AGG + tig0016663:4907-4926 Msa1359200:CDS 40.0%
GCTCAACAAGATCCTCAATA+AGG - tig0016663:4922-4941 Msa1359200:intergenic 40.0%
GTGAAGACAACTTCTTGTGG+TGG + tig0016663:3586-3605 Msa1359200:CDS 45.0%
! TGATAGCGTTAAGGCGTCAA+TGG + tig0016663:4130-4149 Msa1359200:intron 45.0%
! TGCCAGACCCTTTATTTGTG+TGG + tig0016663:4253-4272 Msa1359200:intron 45.0%
! ATGTGAGGCACCTTGTTATG+AGG + tig0016663:4368-4387 Msa1359200:intron 45.0%
! TGTGAGGCACCTTGTTATGA+GGG + tig0016663:4369-4388 Msa1359200:intron 45.0%
!! ACGTAGGAGCTTCTGAAACA+TGG - tig0016663:4429-4448 Msa1359200:intergenic 45.0%
TTCAGAAGCTCCTACGTGTA+AGG + tig0016663:4432-4451 Msa1359200:intron 45.0%
! TAATTTTCGGCTCCCCTTTG+AGG - tig0016663:4627-4646 Msa1359200:intergenic 45.0%
GTGAAGACAACTTCTTGTGG+TGG + tig0016663:3586-3605 Msa1359200:CDS 45.0%
! TGATAGCGTTAAGGCGTCAA+TGG + tig0016663:4130-4149 Msa1359200:intron 45.0%
! TGCCAGACCCTTTATTTGTG+TGG + tig0016663:4253-4272 Msa1359200:intron 45.0%
! ATGTGAGGCACCTTGTTATG+AGG + tig0016663:4368-4387 Msa1359200:intron 45.0%
! TGTGAGGCACCTTGTTATGA+GGG + tig0016663:4369-4388 Msa1359200:intron 45.0%
!! ACGTAGGAGCTTCTGAAACA+TGG - tig0016663:4429-4448 Msa1359200:intergenic 45.0%
TTCAGAAGCTCCTACGTGTA+AGG + tig0016663:4432-4451 Msa1359200:intron 45.0%
! TAATTTTCGGCTCCCCTTTG+AGG - tig0016663:4627-4646 Msa1359200:intergenic 45.0%
GCAGCTTGCAGTGGATATGA+TGG + tig0016663:3639-3658 Msa1359200:CDS 50.0%
GCAGCTTGCAGTGGATATGA+TGG + tig0016663:3639-3658 Msa1359200:CDS 50.0%
TGGGGATGAGCAGCTTGCAG+TGG + tig0016663:3630-3649 Msa1359200:CDS 60.0%
TGGGGATGAGCAGCTTGCAG+TGG + tig0016663:3630-3649 Msa1359200:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0016663 gene 3565 4974 3565 ID=Msa1359200;Name=Msa1359200
tig0016663 mRNA 3565 4974 3565 ID=Msa1359200-mRNA-1;Parent=Msa1359200;Name=Msa1359200-mRNA-1;_AED=0.27;_eAED=0.27;_QI=0|0|0|0.66|0.5|0.66|3|0|113
tig0016663 exon 3565 3681 3565 ID=Msa1359200-mRNA-1:exon:11223;Parent=Msa1359200-mRNA-1
tig0016663 exon 4553 4690 4553 ID=Msa1359200-mRNA-1:exon:11224;Parent=Msa1359200-mRNA-1
tig0016663 exon 4888 4974 4888 ID=Msa1359200-mRNA-1:exon:11225;Parent=Msa1359200-mRNA-1
tig0016663 CDS 3565 3681 3565 ID=Msa1359200-mRNA-1:cds;Parent=Msa1359200-mRNA-1
tig0016663 CDS 4553 4690 4553 ID=Msa1359200-mRNA-1:cds;Parent=Msa1359200-mRNA-1
tig0016663 CDS 4888 4974 4888 ID=Msa1359200-mRNA-1:cds;Parent=Msa1359200-mRNA-1
Gene Sequence

>Msa1359200

TTGAGAACAATTTCTTTCAAAGTGAAGACAACTTCTTGTGGTGGAACACAATGTGTTAACTCTTTTGGGGATGAGCAGCTTGCAGTGGATATGATGGCTAATAATCTTCGTTTTGAGGGTCTAAGTTATCTGCACGGAGTCAGATATTTAGTTCACAGAGACATAAAGCCTGCTAATTTACTTGTAAACCTCAAAGGGGAGCCGAAAATTACTGATTTTGGTATTAGTGTTGGCATAGAGAATTCAGTTGCAATGATAGAAATTGCACTTCAAAAGATGAGCAAGTCCTTATTGAGGATCTTGTTGAGCTGTGAGAGAACGACTAGATTATCTCATTGTTGA

Protein sequence

>Msa1359200

LRTISFKVKTTSCGGTQCVNSFGDEQLAVDMMANNLRFEGLSYLHGVRYLVHRDIKPANLLVNLKGEPKITDFGISVGIENSVAMIEIALQKMSKSLLRILLSCERTTRLSHC*