AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1360030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1360030 MtrunA17_Chr4g0062221 96.429 168 6 0 1 168 1 168 1.25e-117 329
Msa1360030 MtrunA17_Chr8g0368681 71.523 151 43 0 7 157 8 158 2.83e-78 229
Msa1360030 MtrunA17_Chr8g0366401 52.597 154 70 2 3 153 7 160 1.44e-50 159
Msa1360030 MtrunA17_Chr7g0254731 43.972 141 77 1 7 147 8 146 1.25e-36 124
Msa1360030 MtrunA17_Chr7g0254731 46.875 64 34 0 90 153 16 79 1.14e-12 62.0
Msa1360030 MtrunA17_Chr6g0460211 43.262 141 78 1 7 147 8 146 7.94e-36 122
Msa1360030 MtrunA17_Chr6g0460211 46.875 64 34 0 90 153 16 79 1.07e-12 62.4
Msa1360030 MtrunA17_Chr1g0188171 43.056 144 74 2 7 147 8 146 3.43e-35 120
Msa1360030 MtrunA17_Chr1g0188171 37.778 90 53 1 81 167 4 93 1.77e-12 61.6
Msa1360030 MtrunA17_Chr5g0440931 42.553 141 79 1 7 147 8 146 3.92e-35 120
Msa1360030 MtrunA17_Chr5g0440931 46.875 64 34 0 90 153 16 79 9.88e-13 62.4
Msa1360030 MtrunA17_Chr3g0093271 43.262 141 78 1 7 147 59 197 1.14e-34 120
Msa1360030 MtrunA17_Chr3g0093271 46.875 64 34 0 90 153 67 130 2.61e-12 62.4
Msa1360030 MtrunA17_Chr1g0166281 41.844 141 80 1 7 147 8 146 2.94e-33 115
Msa1360030 MtrunA17_Chr1g0166281 43.421 76 39 2 78 150 2 76 2.70e-12 61.2
Msa1360030 MtrunA17_Chr1g0185001 40.690 145 83 2 3 147 4 145 1.13e-28 103
Msa1360030 MtrunA17_Chr7g0274101 34.028 144 93 1 4 147 5 146 8.00e-26 96.3
Msa1360030 MtrunA17_Chr8g0392951 33.562 146 94 2 2 147 15 157 8.67e-23 89.0
Msa1360030 MtrunA17_Chr1g0169581 31.429 140 96 0 10 149 3 142 2.80e-22 87.0
Msa1360030 MtrunA17_Chr3g0100541 35.172 145 77 1 8 152 49 176 4.19e-22 87.4
Msa1360030 MtrunA17_Chr3g0111371 32.143 140 89 2 11 150 5 138 3.23e-21 84.3
Msa1360030 MtrunA17_Chr5g0434951 30.986 142 91 2 10 149 3 139 3.42e-21 84.0
Msa1360030 MtrunA17_Chr8g0391691 33.835 133 86 1 11 143 36 166 1.36e-20 84.0
Msa1360030 MtrunA17_Chr4g0033601 33.553 152 91 3 10 156 4 150 2.29e-20 82.4
Msa1360030 MtrunA17_Chr1g0157131 32.215 149 93 3 7 153 375 517 6.27e-20 85.9
Msa1360030 MtrunA17_Chr5g0398751 32.624 141 91 2 7 147 8 144 6.84e-20 80.9
Msa1360030 MtrunA17_Chr5g0397101 35.616 146 86 4 12 155 372 511 7.61e-20 85.5
Msa1360030 MtrunA17_Chr1g0161211 34.965 143 83 3 12 150 47 183 1.86e-19 81.3
Msa1360030 MtrunA17_Chr1g0161211 47.692 65 34 0 85 149 45 109 1.96e-12 62.4
Msa1360030 MtrunA17_Chr4g0044961 29.577 142 96 1 9 150 55 192 5.32e-19 79.7
Msa1360030 MtrunA17_Chr5g0409441 34.014 147 92 2 4 147 12 156 8.56e-19 78.6
Msa1360030 MtrunA17_Chr3g0100561 34.559 136 72 1 8 143 49 167 1.11e-18 78.6
Msa1360030 MtrunA17_Chr5g0444171 35.211 142 86 3 7 147 393 529 1.35e-18 82.0
Msa1360030 MtrunA17_Chr7g0267751 31.757 148 96 3 7 154 436 578 2.34e-18 81.3
Msa1360030 MtrunA17_Chr2g0329371 34.266 143 86 3 10 147 63 202 3.30e-18 78.2
Msa1360030 MtrunA17_Chr0c02g0489761 34.058 138 87 3 12 149 384 517 3.63e-18 80.9
Msa1360030 MtrunA17_Chr3g0098951 34.694 147 87 3 7 147 384 527 4.05e-18 80.5
Msa1360030 MtrunA17_Chr7g0245231 29.605 152 97 3 11 162 30 171 4.30e-18 77.0
Msa1360030 MtrunA17_Chr8g0387671 35.172 145 86 4 12 154 372 510 4.64e-18 80.5
Msa1360030 MtrunA17_Chr4g0074811 34.965 143 85 3 7 147 392 528 6.75e-18 80.1
Msa1360030 MtrunA17_Chr1g0200601 30.667 150 99 3 7 156 331 475 8.64e-18 79.7
Msa1360030 MtrunA17_Chr7g0225921 32.867 143 92 3 6 147 1 140 1.12e-17 75.1
Msa1360030 MtrunA17_Chr7g0256171 32.168 143 93 2 11 151 5 145 1.12e-17 75.5
Msa1360030 MtrunA17_Chr8g0385341 32.168 143 92 3 7 149 331 468 1.72e-17 79.0
Msa1360030 MtrunA17_Chr6g0459231 29.577 142 97 1 6 147 3 141 1.93e-17 74.3
Msa1360030 MtrunA17_Chr1g0160151 34.266 143 86 2 10 147 21 160 2.11e-17 75.1
Msa1360030 MtrunA17_Chr5g0402331 30.496 141 90 4 10 147 47 182 6.16e-17 74.3
Msa1360030 MtrunA17_Chr5g0448721 30.769 143 94 3 7 149 397 534 6.96e-17 77.0
Msa1360030 MtrunA17_Chr7g0242561 31.206 141 94 2 7 147 357 494 7.91e-17 77.0
Msa1360030 MtrunA17_Chr3g0100511 32.353 136 75 1 8 143 49 167 8.77e-17 73.6
Msa1360030 MtrunA17_Chr6g0482731 27.660 141 98 2 9 148 24 161 1.49e-16 72.8
Msa1360030 MtrunA17_Chr5g0441691 31.884 138 90 3 12 149 374 507 2.63e-16 75.5
Msa1360030 MtrunA17_Chr3g0100501 42.466 73 42 0 81 153 67 139 2.64e-16 71.6
Msa1360030 MtrunA17_Chr4g0064231 30.061 163 98 2 11 157 64 226 2.68e-16 73.6
Msa1360030 MtrunA17_Chr6g0450921 28.369 141 99 2 11 151 29 167 4.37e-16 71.6
Msa1360030 MtrunA17_Chr5g0402361 30.496 141 90 4 10 147 219 354 4.52e-16 74.3
Msa1360030 MtrunA17_Chr5g0402361 28.261 138 96 1 10 147 47 181 9.85e-16 73.6
Msa1360030 MtrunA17_Chr1g0169621 28.659 164 92 3 10 148 3 166 4.92e-16 71.6
Msa1360030 MtrunA17_Chr1g0170281 28.667 150 102 2 7 156 419 563 5.15e-16 74.7
Msa1360030 MtrunA17_Chr3g0131181 30.070 143 95 3 7 149 330 467 5.20e-16 74.7
Msa1360030 MtrunA17_Chr4g0059951 31.469 143 93 3 6 147 373 511 7.26e-16 74.3
Msa1360030 MtrunA17_Chr1g0176751 29.054 148 100 3 7 154 425 567 9.92e-16 73.9
Msa1360030 MtrunA17_Chr7g0245911 30.625 160 101 5 2 153 38 195 2.38e-15 70.1
Msa1360030 MtrunA17_Chr7g0256701 30.667 150 101 3 1 147 63 212 4.27e-15 70.1
Msa1360030 MtrunA17_Chr4g0057661 31.579 133 88 1 15 147 10 139 5.03e-15 68.2
Msa1360030 MtrunA17_Chr3g0116141 32.331 133 87 2 15 147 9 138 5.19e-15 68.2
Msa1360030 MtrunA17_Chr3g0116151 32.331 133 87 2 15 147 10 139 5.96e-15 67.8
Msa1360030 MtrunA17_Chr4g0057651 29.927 137 93 1 11 147 6 139 2.29e-14 66.2
Msa1360030 MtrunA17_Chr8g0351681 29.371 143 95 3 11 147 49 191 7.43e-14 66.2
Msa1360030 MtrunA17_Chr4g0057641 32.624 141 84 4 11 147 6 139 1.73e-13 63.9
Msa1360030 MtrunA17_Chr1g0173541 29.655 145 97 3 4 147 350 490 7.58e-13 65.5
Msa1360030 MtrunA17_Chr3g0100461 36.250 80 44 2 73 149 33 108 1.21e-12 61.2
Msa1360030 MtrunA17_Chr4g0074791 28.571 147 91 3 1 147 390 522 4.43e-12 63.2
Msa1360030 MtrunA17_Chr7g0257751 27.083 144 102 1 4 147 356 496 1.22e-11 62.0
Msa1360030 MtrunA17_Chr4g0068501 26.429 140 101 1 11 148 16 155 2.75e-11 58.9
Msa1360030 MtrunA17_Chr1g0176421 28.346 127 86 3 7 133 462 583 3.47e-11 60.8
Msa1360030 MtrunA17_Chr8g0373691 26.812 138 97 2 12 148 54 188 3.88e-11 58.9
Msa1360030 MtrunA17_Chr4g0033371 28.873 142 95 3 7 147 373 509 4.18e-11 60.5
Msa1360030 MtrunA17_Chr5g0405421 30.323 155 97 4 2 152 397 544 7.91e-11 59.7
Msa1360030 MtrunA17_Chr7g0245371 29.032 155 92 4 11 151 24 174 9.53e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1360030 AT1G18530.1 74.834 151 38 0 7 157 3 153 3.59e-81 237
Msa1360030 AT3G25600.1 67.949 156 50 0 2 157 3 158 6.03e-76 224
Msa1360030 AT1G32250.1 50.323 155 73 1 3 153 8 162 2.08e-47 152
Msa1360030 AT3G03000.1 51.974 152 72 1 2 153 11 161 2.55e-41 136
Msa1360030 AT3G43810.1 43.262 141 78 1 7 147 8 146 1.10e-35 122
Msa1360030 AT3G43810.1 46.875 64 34 0 90 153 16 79 1.48e-12 62.4
Msa1360030 AT1G66410.1 43.262 141 78 1 7 147 8 146 1.67e-35 121
Msa1360030 AT1G66410.1 45.312 64 35 0 90 153 16 79 4.31e-12 60.8
Msa1360030 AT5G37780.1 43.262 141 78 1 7 147 8 146 1.67e-35 121
Msa1360030 AT5G37780.1 45.312 64 35 0 90 153 16 79 4.31e-12 60.8
Msa1360030 AT3G56800.1 42.553 141 79 1 7 147 8 146 3.18e-35 120
Msa1360030 AT3G56800.1 46.875 64 34 0 90 153 16 79 1.36e-12 62.4
Msa1360030 AT2G41110.1 42.553 141 79 1 7 147 8 146 3.18e-35 120
Msa1360030 AT2G41110.1 46.875 64 34 0 90 153 16 79 1.36e-12 62.4
Msa1360030 AT2G27030.1 42.553 141 79 1 7 147 8 146 3.18e-35 120
Msa1360030 AT2G27030.1 46.875 64 34 0 90 153 16 79 1.36e-12 62.4
Msa1360030 AT3G43810.2 43.262 141 78 1 7 147 8 146 3.72e-35 122
Msa1360030 AT3G43810.2 46.875 64 34 0 90 153 16 79 2.22e-12 63.2
Msa1360030 AT5G21274.1 42.553 141 79 1 7 147 8 146 4.08e-35 120
Msa1360030 AT5G21274.1 46.875 64 34 0 90 153 16 79 1.51e-12 62.4
Msa1360030 AT2G27030.3 42.553 141 79 1 7 147 8 146 4.72e-35 121
Msa1360030 AT2G27030.3 46.875 64 34 0 90 153 16 79 3.01e-12 62.0
Msa1360030 AT4G14640.1 41.667 144 76 2 7 147 9 147 2.28e-34 118
Msa1360030 AT3G22930.1 42.553 141 79 2 7 147 31 169 6.48e-33 115
Msa1360030 AT3G22930.1 44.444 72 37 1 82 150 28 99 4.30e-11 58.9
Msa1360030 AT2G41110.2 39.869 153 78 2 7 147 8 158 2.76e-31 110
Msa1360030 AT5G37780.2 39.103 156 78 2 7 147 8 161 4.77e-31 110
Msa1360030 AT5G37780.3 36.527 167 78 2 7 147 8 172 1.71e-29 107
Msa1360030 AT3G51920.1 38.298 141 85 1 7 147 8 146 2.73e-29 105
Msa1360030 AT1G66410.2 40.397 151 78 2 7 147 8 156 8.88e-29 104
Msa1360030 AT5G37780.4 43.089 123 68 1 25 147 13 133 2.37e-28 102
Msa1360030 AT4G14640.2 41.270 126 66 2 25 147 9 129 1.45e-27 100
Msa1360030 AT1G66410.4 43.750 112 61 1 36 147 1 110 5.24e-26 95.9
Msa1360030 AT1G66410.3 43.750 112 61 1 36 147 1 110 5.24e-26 95.9
Msa1360030 AT3G43810.4 42.857 112 62 1 36 147 1 110 1.30e-25 95.1
Msa1360030 AT3G43810.3 42.857 112 62 1 36 147 1 110 1.30e-25 95.1
Msa1360030 AT2G41100.7 32.919 161 93 2 3 148 58 218 1.72e-23 94.0
Msa1360030 AT2G41100.7 30.000 130 77 2 35 150 1 130 6.95e-14 68.2
Msa1360030 AT2G41100.6 32.919 161 93 2 3 148 58 218 1.72e-23 94.0
Msa1360030 AT2G41100.6 30.000 130 77 2 35 150 1 130 6.95e-14 68.2
Msa1360030 AT2G41100.3 32.919 161 93 2 3 148 58 218 1.72e-23 94.0
Msa1360030 AT2G41100.3 30.000 130 77 2 35 150 1 130 6.95e-14 68.2
Msa1360030 AT2G41100.4 32.919 161 93 2 3 148 93 253 2.86e-23 94.0
Msa1360030 AT2G41100.4 32.716 162 95 2 3 150 4 165 4.02e-23 93.6
Msa1360030 AT2G41100.1 32.919 161 93 2 3 148 93 253 2.86e-23 94.0
Msa1360030 AT2G41100.1 32.716 162 95 2 3 150 4 165 4.02e-23 93.6
Msa1360030 AT4G37010.1 34.043 141 91 1 11 151 27 165 3.02e-23 90.5
Msa1360030 AT4G37010.2 34.043 141 91 1 11 151 31 169 3.40e-23 90.5
Msa1360030 AT2G41090.1 35.616 146 89 1 2 147 3 143 1.92e-22 89.0
Msa1360030 AT5G37770.1 30.345 145 99 1 9 151 15 159 3.64e-21 85.1
Msa1360030 AT5G37770.2 30.345 145 99 1 9 151 15 159 3.64e-21 85.1
Msa1360030 AT1G24620.1 33.333 141 92 1 8 148 34 172 1.14e-20 84.3
Msa1360030 AT1G05990.1 34.507 142 84 3 11 147 5 142 3.27e-20 82.4
Msa1360030 AT2G15680.1 29.286 140 95 1 9 148 48 183 4.13e-20 82.8
Msa1360030 AT4G14640.3 40.385 104 54 2 7 107 9 107 6.98e-20 80.5
Msa1360030 AT1G18210.2 28.859 149 98 2 2 148 12 154 1.82e-19 80.9
Msa1360030 AT1G18210.1 28.859 149 98 2 2 148 12 154 1.82e-19 80.9
Msa1360030 AT3G22930.2 41.176 102 58 2 7 108 31 130 2.37e-19 79.3
Msa1360030 AT3G22930.2 45.070 71 36 1 82 149 28 98 1.78e-11 58.9
Msa1360030 AT3G50360.1 30.496 141 96 1 11 151 27 165 2.91e-19 80.5
Msa1360030 AT4G03290.1 35.417 144 86 3 11 149 5 146 3.08e-19 79.7
Msa1360030 AT1G35670.1 32.867 143 91 3 7 149 327 464 4.67e-19 83.6
Msa1360030 AT2G43290.1 32.394 142 91 2 11 147 65 206 1.27e-18 79.7
Msa1360030 AT5G17470.1 32.374 139 86 2 14 148 5 139 1.91e-18 77.4
Msa1360030 AT1G76040.1 32.215 149 93 3 7 153 174 316 2.13e-18 80.9
Msa1360030 AT1G76040.4 32.215 149 93 3 7 153 174 316 2.13e-18 80.9
Msa1360030 AT1G74740.1 32.168 143 92 3 6 147 359 497 2.37e-18 81.6
Msa1360030 AT4G12860.1 33.094 139 89 2 11 147 5 141 2.51e-18 77.4
Msa1360030 AT1G12310.1 30.496 141 94 2 7 147 9 145 3.39e-18 77.0
Msa1360030 AT4G38230.1 31.469 143 93 3 7 149 181 318 3.70e-18 80.5
Msa1360030 AT1G61950.1 34.247 146 88 3 4 147 397 536 4.34e-18 80.9
Msa1360030 AT1G66400.1 30.882 136 94 0 12 147 16 151 4.40e-18 77.0
Msa1360030 AT1G76040.2 32.215 149 93 3 7 153 412 554 4.45e-18 80.9
Msa1360030 AT4G38230.4 31.469 143 93 3 7 149 325 462 5.29e-18 80.5
Msa1360030 AT4G38230.3 31.469 143 93 3 7 149 325 462 5.29e-18 80.5
Msa1360030 AT4G09570.1 31.469 143 93 2 7 149 326 463 5.41e-18 80.5
Msa1360030 AT4G38230.2 31.469 143 93 3 7 149 355 492 6.27e-18 80.5
Msa1360030 AT1G62820.1 29.787 141 95 2 7 147 9 145 7.45e-18 76.3
Msa1360030 AT5G12180.1 32.639 144 93 2 12 155 379 518 1.44e-17 79.3
Msa1360030 AT5G19360.1 32.394 142 92 2 12 153 374 511 1.81e-17 79.3
Msa1360030 AT3G51850.1 30.282 142 96 1 6 147 354 492 2.43e-17 79.0
Msa1360030 AT4G35310.2 31.469 143 93 3 7 149 398 535 2.77e-17 78.6
Msa1360030 AT4G35310.1 31.469 143 93 3 7 149 398 535 2.77e-17 78.6
Msa1360030 AT4G04695.1 33.333 150 91 4 7 154 332 474 4.67e-17 77.8
Msa1360030 AT5G23580.1 31.469 143 93 3 7 149 323 460 1.07e-16 77.0
Msa1360030 AT4G04720.2 33.784 148 89 4 7 152 380 520 1.09e-16 77.0
Msa1360030 AT4G04720.1 33.784 148 89 4 7 152 380 520 1.09e-16 77.0
Msa1360030 AT1G50700.1 30.822 146 93 3 4 147 371 510 2.46e-16 75.9
Msa1360030 AT4G23650.1 34.247 146 88 4 12 155 384 523 2.47e-16 75.9
Msa1360030 AT2G17290.1 30.070 143 95 3 7 149 386 523 2.54e-16 75.9
Msa1360030 AT2G17290.2 30.070 143 95 3 7 149 386 523 2.54e-16 75.9
Msa1360030 AT4G04700.1 32.192 146 95 2 7 152 332 473 2.61e-16 75.9
Msa1360030 AT2G41410.1 33.557 149 96 3 7 153 66 213 3.54e-16 73.2
Msa1360030 AT1G73630.1 26.087 138 96 2 11 148 20 151 5.74e-16 71.6
Msa1360030 AT4G04710.3 34.266 143 86 3 7 147 319 455 5.85e-16 74.7
Msa1360030 AT4G04710.2 34.266 143 86 3 7 147 333 469 6.92e-16 74.7
Msa1360030 AT4G04710.1 34.266 143 86 3 7 147 346 482 8.24e-16 74.3
Msa1360030 AT3G20410.2 31.507 146 92 3 4 147 389 528 8.38e-16 74.3
Msa1360030 AT3G20410.1 31.507 146 92 3 4 147 389 528 8.38e-16 74.3
Msa1360030 AT3G59440.1 33.333 141 83 5 12 147 52 186 9.08e-16 71.6
Msa1360030 AT4G21940.1 32.168 143 89 3 7 147 402 538 1.49e-15 73.6
Msa1360030 AT4G21940.2 32.168 143 89 3 7 147 402 538 1.82e-15 73.6
Msa1360030 AT2G41100.2 30.952 126 72 2 38 148 3 128 5.80e-15 69.7
Msa1360030 AT2G36180.1 27.737 137 98 1 12 147 1 137 6.55e-15 68.2
Msa1360030 AT3G50770.1 32.168 143 90 4 11 148 64 204 7.90e-15 69.3
Msa1360030 AT5G04870.1 27.586 145 100 3 7 151 451 590 9.62e-15 71.2
Msa1360030 AT2G41860.1 31.034 145 95 3 4 147 352 492 1.74e-14 70.5
Msa1360030 AT2G41860.2 31.034 145 95 3 4 147 352 492 1.74e-14 70.5
Msa1360030 AT3G10190.1 28.777 139 94 3 11 147 70 205 1.93e-14 68.6
Msa1360030 AT4G04710.4 33.793 145 86 4 7 147 346 484 2.28e-14 70.1
Msa1360030 AT4G04740.4 31.469 143 90 3 7 147 369 505 2.31e-14 70.1
Msa1360030 AT4G04740.6 31.469 143 90 3 7 147 369 505 2.31e-14 70.1
Msa1360030 AT4G04740.5 31.469 143 90 3 7 147 369 505 2.31e-14 70.1
Msa1360030 AT4G04740.3 31.469 143 90 3 7 147 369 505 2.31e-14 70.1
Msa1360030 AT4G04740.1 31.469 143 90 3 7 147 369 505 2.31e-14 70.1
Msa1360030 AT4G04740.7 31.469 143 90 3 7 147 369 505 2.31e-14 70.1
Msa1360030 AT1G18890.1 31.469 143 93 3 6 147 363 501 3.30e-14 69.7
Msa1360030 AT2G38910.1 29.139 151 102 3 7 157 435 580 3.58e-14 69.7
Msa1360030 AT3G10660.2 27.586 145 100 3 7 151 487 626 4.03e-14 69.7
Msa1360030 AT3G10660.1 27.586 145 100 3 7 151 487 626 4.03e-14 69.7
Msa1360030 AT5G42380.1 30.216 139 96 1 9 147 47 184 4.23e-14 67.0
Msa1360030 AT3G07490.1 34.211 152 88 5 11 156 5 150 6.27e-14 65.9
Msa1360030 AT2G41100.5 50.000 58 29 0 91 148 17 74 9.47e-14 65.5
Msa1360030 AT3G57530.1 34.058 138 83 5 12 147 369 500 3.73e-13 66.6
Msa1360030 AT3G03400.1 27.907 129 93 0 12 140 6 134 5.73e-13 63.2
Msa1360030 AT2G31500.1 31.579 152 95 6 7 154 367 513 2.39e-12 64.3
Msa1360030 AT3G03410.1 27.407 135 87 1 13 147 4 127 5.15e-12 60.5
Msa1360030 AT1G50700.2 25.581 172 94 4 4 147 371 536 5.23e-12 63.5
Msa1360030 AT4G36070.2 34.000 150 88 4 7 152 374 516 7.07e-12 63.2
Msa1360030 AT4G36070.1 33.553 152 86 5 7 152 374 516 7.48e-12 62.8
Msa1360030 AT1G21550.1 27.778 144 98 4 10 147 9 152 8.42e-12 60.5
Msa1360030 AT3G51850.2 29.091 110 75 1 6 115 354 460 9.29e-12 62.8

Find 30 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAATCTTATTAGCCAAAA+TGG 0.199928 tig0016871:-70320 Msa1360030:CDS
GTTTCTGTTTGAATCCATTT+TGG 0.223500 tig0016871:+70306 None:intergenic
TGCATTCATGTTCGGCATTA+TGG 0.231984 tig0016871:+70252 None:intergenic
CGCTCTGCTTCTGATTTATT+AGG 0.273541 tig0016871:-70019 Msa1360030:CDS
CTGCTAGTTCTAACATTGTT+AGG 0.317076 tig0016871:+70386 None:intergenic
ATTCATTTCAGCAGCTGAAT+TGG 0.352081 tig0016871:-70164 Msa1360030:CDS
GATGATTAGAGAAGCTGATA+TGG 0.368224 tig0016871:-70089 Msa1360030:CDS
GGGTCAGCCACTTACATATA+AGG 0.372815 tig0016871:-70122 Msa1360030:CDS
TCGTTAGGGCTAAAGCCTTC+AGG 0.374738 tig0016871:-70352 Msa1360030:CDS
GCAGCACTTATTCGTTCGTT+AGG 0.382441 tig0016871:-70367 Msa1360030:CDS
TAGTGAGTTTGCTACAATCA+TGG 0.443483 tig0016871:-70044 Msa1360030:CDS
CAGCACTTATTCGTTCGTTA+GGG 0.460217 tig0016871:-70366 Msa1360030:CDS
ATTTCAGCAGCTGAATTGGC+TGG 0.463901 tig0016871:-70160 Msa1360030:CDS
AATACTTCTGCATTCATGTT+CGG 0.467152 tig0016871:+70244 None:intergenic
TGCTTCGATCGCGATGGGAA+TGG 0.468041 tig0016871:-70187 Msa1360030:CDS
GTGAATCAAGAACAACTCAT+AGG 0.474821 tig0016871:-70220 Msa1360030:CDS
ATAAGCTCCTTATATGTAAG+TGG 0.483364 tig0016871:+70115 None:intergenic
AGCTGAATTGGCTGGAGCAA+TGG 0.497837 tig0016871:-70152 Msa1360030:CDS
GGATTCAAACAGAAACGGTT+CGG 0.505680 tig0016871:-70299 Msa1360030:CDS
TCAAATGCTTCGATCGCGAT+GGG 0.540427 tig0016871:-70192 Msa1360030:CDS
GATATTAACTAATATGTCAA+AGG 0.549728 tig0016871:-70476 None:intergenic
TTCAAATGCTTCGATCGCGA+TGG 0.556995 tig0016871:-70193 Msa1360030:CDS
AAAATGGATTCAAACAGAAA+CGG 0.570631 tig0016871:-70304 Msa1360030:CDS
TAACATTGTTAGGCTGCCAT+CGG 0.573272 tig0016871:+70396 None:intergenic
ATTTGAACTTCGTCACCTGA+AGG 0.579483 tig0016871:+70337 None:intergenic
GAACATGAATGCAGAAGTAT+TGG 0.581543 tig0016871:-70242 Msa1360030:CDS
AATCTTCAGTCGATTCGACA+TGG 0.588153 tig0016871:-70422 Msa1360030:CDS
CGATTCGACATGGATTCCGA+TGG 0.648654 tig0016871:-70412 Msa1360030:CDS
ATTAGAGAAGCTGATATGGA+TGG 0.660029 tig0016871:-70085 Msa1360030:CDS
GAAGCTGATATGGATGGTGA+TGG 0.666205 tig0016871:-70079 Msa1360030:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TCAAATCTTATTAGCCAAAA+TGG - tig0016871:70119-70138 Msa1360030:CDS 25.0%
!! AAAATGGATTCAAACAGAAA+CGG - tig0016871:70135-70154 Msa1360030:CDS 25.0%
! GTTTCTGTTTGAATCCATTT+TGG + tig0016871:70136-70155 None:intergenic 30.0%
AATACTTCTGCATTCATGTT+CGG + tig0016871:70198-70217 None:intergenic 30.0%
ATAAGCTCCTTATATGTAAG+TGG + tig0016871:70327-70346 None:intergenic 30.0%
!! CTGCTAGTTCTAACATTGTT+AGG + tig0016871:70056-70075 None:intergenic 35.0%
! GAACATGAATGCAGAAGTAT+TGG - tig0016871:70197-70216 Msa1360030:CDS 35.0%
GTGAATCAAGAACAACTCAT+AGG - tig0016871:70219-70238 Msa1360030:CDS 35.0%
ATTCATTTCAGCAGCTGAAT+TGG - tig0016871:70275-70294 Msa1360030:CDS 35.0%
GATGATTAGAGAAGCTGATA+TGG - tig0016871:70350-70369 Msa1360030:CDS 35.0%
ATTAGAGAAGCTGATATGGA+TGG - tig0016871:70354-70373 Msa1360030:CDS 35.0%
!! TAGTGAGTTTGCTACAATCA+TGG - tig0016871:70395-70414 Msa1360030:CDS 35.0%
!! AATCTTCAGTCGATTCGACA+TGG - tig0016871:70017-70036 Msa1360030:CDS 40.0%
TAACATTGTTAGGCTGCCAT+CGG + tig0016871:70046-70065 None:intergenic 40.0%
CAGCACTTATTCGTTCGTTA+GGG - tig0016871:70073-70092 Msa1360030:CDS 40.0%
ATTTGAACTTCGTCACCTGA+AGG + tig0016871:70105-70124 None:intergenic 40.0%
GGATTCAAACAGAAACGGTT+CGG - tig0016871:70140-70159 Msa1360030:CDS 40.0%
TGCATTCATGTTCGGCATTA+TGG + tig0016871:70190-70209 None:intergenic 40.0%
CGCTCTGCTTCTGATTTATT+AGG - tig0016871:70420-70439 Msa1360030:CDS 40.0%
GCAGCACTTATTCGTTCGTT+AGG - tig0016871:70072-70091 Msa1360030:CDS 45.0%
TTCAAATGCTTCGATCGCGA+TGG - tig0016871:70246-70265 Msa1360030:CDS 45.0%
TCAAATGCTTCGATCGCGAT+GGG - tig0016871:70247-70266 Msa1360030:CDS 45.0%
ATTTCAGCAGCTGAATTGGC+TGG - tig0016871:70279-70298 Msa1360030:CDS 45.0%
GCTGGAGCAATGGCAAAAAT+GGG - tig0016871:70297-70316 Msa1360030:CDS 45.0%
GGGTCAGCCACTTACATATA+AGG - tig0016871:70317-70336 Msa1360030:CDS 45.0%
GAAGCTGATATGGATGGTGA+TGG - tig0016871:70360-70379 Msa1360030:CDS 45.0%
CGATTCGACATGGATTCCGA+TGG - tig0016871:70027-70046 Msa1360030:CDS 50.0%
TCGTTAGGGCTAAAGCCTTC+AGG - tig0016871:70087-70106 Msa1360030:CDS 50.0%
AGCTGAATTGGCTGGAGCAA+TGG - tig0016871:70287-70306 Msa1360030:CDS 50.0%
GGCTGGAGCAATGGCAAAAA+TGG - tig0016871:70296-70315 Msa1360030:CDS 50.0%
TGCTTCGATCGCGATGGGAA+TGG - tig0016871:70252-70271 Msa1360030:CDS 55.0%
Chromosome Type Strat End Strand Name
tig0016871 gene 69976 70485 69976 ID=Msa1360030;Name=Msa1360030
tig0016871 mRNA 69976 70485 69976 ID=Msa1360030-mRNA-1;Parent=Msa1360030;Name=Msa1360030-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|169
tig0016871 exon 69976 70485 69976 ID=Msa1360030-mRNA-1:exon:11672;Parent=Msa1360030-mRNA-1
tig0016871 CDS 69976 70485 69976 ID=Msa1360030-mRNA-1:cds;Parent=Msa1360030-mRNA-1
Gene Sequence

>Msa1360030

ATGTCAAAGGTTAAAGTTGATCAACTTAATCAACTAAGAGAAATCTTCAGTCGATTCGACATGGATTCCGATGGCAGCCTAACAATGTTAGAACTAGCAGCACTTATTCGTTCGTTAGGGCTAAAGCCTTCAGGTGACGAAGTTCAAATCTTATTAGCCAAAATGGATTCAAACAGAAACGGTTCGGTTGAGTTCGATGAATTAGTAGAAGCCATAATGCCGAACATGAATGCAGAAGTATTGGTGAATCAAGAACAACTCATAGGTGTGTTCAAATGCTTCGATCGCGATGGGAATGGATTCATTTCAGCAGCTGAATTGGCTGGAGCAATGGCAAAAATGGGTCAGCCACTTACATATAAGGAGCTTATTGAGATGATTAGAGAAGCTGATATGGATGGTGATGGTGTTATTAGTTTTAGTGAGTTTGCTACAATCATGGCTCGCTCTGCTTCTGATTTATTAGGAGTGTTTGAATACAGACACAAAACACATTCATTTCAATTATAA
Protein sequence

>Msa1360030

MSKVKVDQLNQLREIFSRFDMDSDGSLTMLELAALIRSLGLKPSGDEVQILLAKMDSNRNGSVEFDELVEAIMPNMNAEVLVNQEQLIGVFKCFDRDGNGFISAAELAGAMAKMGQPLTYKELIEMIREADMDGDGVISFSEFATIMARSASDLLGVFEYRHKTHSFQL*