Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1367930 | XP_003622911.2 | 88.375 | 929 | 36 | 3 | 1 | 887 | 1 | 899 | 0.0 | 1656 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1367930 | sp|C0LGV0|Y5487_ARATH | 57.826 | 920 | 313 | 6 | 10 | 887 | 4 | 890 | 0.0 | 1034 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1017600 | Msa1367930 | 0.868191 | 7.843436e-66 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1367930 | MtrunA17_Chr7g0236881 | 87.601 | 863 | 35 | 3 | 1 | 821 | 1 | 833 | 0.0 | 1524 |
| Msa1367930 | MtrunA17_Chr3g0123141 | 32.379 | 908 | 514 | 26 | 27 | 881 | 25 | 885 | 5.61e-129 | 410 |
| Msa1367930 | MtrunA17_Chr7g0263591 | 32.684 | 924 | 482 | 30 | 26 | 881 | 32 | 883 | 1.19e-128 | 410 |
| Msa1367930 | MtrunA17_Chr1g0181731 | 31.562 | 922 | 525 | 25 | 21 | 891 | 21 | 887 | 1.04e-125 | 402 |
| Msa1367930 | MtrunA17_Chr8g0341151 | 31.838 | 914 | 501 | 32 | 9 | 882 | 6 | 837 | 5.57e-115 | 372 |
| Msa1367930 | MtrunA17_Chr8g0340871 | 32.220 | 928 | 482 | 35 | 9 | 882 | 18 | 852 | 1.04e-112 | 366 |
| Msa1367930 | MtrunA17_Chr5g0411631 | 31.789 | 928 | 496 | 27 | 16 | 881 | 23 | 875 | 5.44e-111 | 362 |
| Msa1367930 | MtrunA17_Chr8g0340911 | 31.779 | 922 | 487 | 32 | 9 | 882 | 16 | 843 | 7.73e-111 | 361 |
| Msa1367930 | MtrunA17_Chr8g0341011 | 30.619 | 921 | 473 | 34 | 24 | 882 | 31 | 847 | 1.13e-110 | 360 |
| Msa1367930 | MtrunA17_Chr8g0340881 | 30.744 | 927 | 501 | 32 | 9 | 882 | 10 | 848 | 1.88e-110 | 360 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1367930 | AT5G48740.1 | 57.826 | 920 | 313 | 6 | 10 | 887 | 4 | 890 | 0.0 | 1034 |
| Msa1367930 | AT1G67720.1 | 31.710 | 924 | 519 | 23 | 16 | 881 | 14 | 883 | 3.13e-127 | 406 |
| Msa1367930 | AT2G37050.3 | 33.406 | 913 | 508 | 28 | 26 | 887 | 27 | 890 | 3.71e-120 | 387 |
| Msa1367930 | AT2G37050.1 | 33.406 | 913 | 507 | 29 | 26 | 887 | 27 | 889 | 1.08e-118 | 384 |
| Msa1367930 | AT2G28990.2 | 32.896 | 915 | 480 | 33 | 17 | 877 | 15 | 849 | 3.81e-118 | 381 |
| Msa1367930 | AT2G28990.1 | 32.896 | 915 | 480 | 33 | 17 | 877 | 15 | 849 | 3.81e-118 | 381 |
| Msa1367930 | AT2G19190.1 | 32.462 | 918 | 488 | 34 | 9 | 880 | 18 | 849 | 9.46e-118 | 380 |
| Msa1367930 | AT4G29990.1 | 33.588 | 917 | 474 | 39 | 13 | 877 | 12 | 845 | 1.40e-114 | 371 |
| Msa1367930 | AT2G19210.1 | 31.901 | 931 | 486 | 36 | 6 | 877 | 8 | 849 | 3.06e-113 | 368 |
| Msa1367930 | AT3G46400.1 | 31.853 | 923 | 491 | 35 | 14 | 877 | 5 | 848 | 2.06e-111 | 363 |
Find 205 sgRNAs with CRISPR-Local
Find 415 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACCAAATTGAATGAATC+AGG | 0.380707 | tig0018573:-21871 | None:intergenic |
| AAAAGTGATGTATATAGTTT+TGG | 0.321882 | tig0018573:+21793 | Msa1367930:CDS |
| AAACTAGTGGCATTGTTCAT+TGG | 0.413279 | tig0018573:-17376 | None:intergenic |
| AAAGGAATCAACCCCAAGTT+GGG | 0.476493 | tig0018573:-20833 | None:intergenic |
| AACCATCTAGCAATAATACT+AGG | 0.587741 | tig0018573:+20464 | Msa1367930:CDS |
| AACCCACTTGTAGCATGAAA+AGG | 0.487033 | tig0018573:-18185 | None:intergenic |
| AACCTACCTCTTGACGGGTT+AGG | 0.421205 | tig0018573:+18311 | Msa1367930:CDS |
| AACCTTCGAATCCAGCTTAA+GGG | 0.366810 | tig0018573:-21354 | None:intergenic |
| AAGAAACTGATATGAGGAAC+TGG | 0.421437 | tig0018573:+20662 | Msa1367930:CDS |
| AAGAAGTTTGATTCTTCTCA+CGG | 0.548064 | tig0018573:+22669 | Msa1367930:three_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TCATGGATTTAAGAGATTCA+TGG | + | tig0018573:16705-16724 | Msa1367930:exon | 30.0% | |
| CTTATATACTCCTGATTTCA+TGG | + | tig0018573:17055-17074 | Msa1367930:intron | 30.0% | |
| CTTGTTGAATTTCATGAAAC+AGG | + | tig0018573:17166-17185 | Msa1367930:intron | 30.0% | |
| TCTAACAAGAATCAAACTAG+TGG | - | tig0018573:17392-17411 | None:intergenic | 30.0% | |
| AGAGAAACATAGAAAATAGG+CGG | - | tig0018573:17454-17473 | None:intergenic | 30.0% | |
| AGATTCATAGTGATTGGATT+TGG | - | tig0018573:17863-17882 | None:intergenic | 30.0% | |
| TGTCTGACTACACTAAATTT+AGG | - | tig0018573:17990-18009 | None:intergenic | 30.0% | |
| TCTTAAAAACAGGTATCCTA+TGG | + | tig0018573:18121-18140 | Msa1367930:intron | 30.0% | |
| TGCATTCGAGGTATATAATA+TGG | + | tig0018573:18529-18548 | Msa1367930:CDS | 30.0% | |
| GTTGGAAAGTTCAAAAGATA+AGG | - | tig0018573:18599-18618 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0018573 | gene | 16629 | 22872 | 16629 | ID=Msa1367930;Name=Msa1367930 |
| tig0018573 | mRNA | 16629 | 22872 | 16629 | ID=Msa1367930-mRNA-1;Parent=Msa1367930;Name=Msa1367930-mRNA-1;_AED=0.12;_eAED=0.12;_QI=78|0.84|0.92|1|0.92|0.85|14|216|892 |
| tig0018573 | exon | 16629 | 16782 | 16629 | ID=Msa1367930-mRNA-1:exon:196;Parent=Msa1367930-mRNA-1 |
| tig0018573 | exon | 17188 | 17728 | 17188 | ID=Msa1367930-mRNA-1:exon:197;Parent=Msa1367930-mRNA-1 |
| tig0018573 | exon | 18133 | 18584 | 18133 | ID=Msa1367930-mRNA-1:exon:198;Parent=Msa1367930-mRNA-1 |
| tig0018573 | exon | 18882 | 18999 | 18882 | ID=Msa1367930-mRNA-1:exon:199;Parent=Msa1367930-mRNA-1 |
| tig0018573 | exon | 19534 | 19602 | 19534 | ID=Msa1367930-mRNA-1:exon:200;Parent=Msa1367930-mRNA-1 |
| tig0018573 | exon | 19684 | 19752 | 19684 | ID=Msa1367930-mRNA-1:exon:201;Parent=Msa1367930-mRNA-1 |
| tig0018573 | exon | 19864 | 19935 | 19864 | ID=Msa1367930-mRNA-1:exon:202;Parent=Msa1367930-mRNA-1 |
| tig0018573 | exon | 20063 | 20131 | 20063 | ID=Msa1367930-mRNA-1:exon:203;Parent=Msa1367930-mRNA-1 |
| tig0018573 | exon | 20346 | 20524 | 20346 | ID=Msa1367930-mRNA-1:exon:204;Parent=Msa1367930-mRNA-1 |
| tig0018573 | exon | 20659 | 20864 | 20659 | ID=Msa1367930-mRNA-1:exon:205;Parent=Msa1367930-mRNA-1 |
| tig0018573 | exon | 21104 | 21230 | 21104 | ID=Msa1367930-mRNA-1:exon:206;Parent=Msa1367930-mRNA-1 |
| tig0018573 | exon | 21334 | 21682 | 21334 | ID=Msa1367930-mRNA-1:exon:207;Parent=Msa1367930-mRNA-1 |
| tig0018573 | exon | 21765 | 21897 | 21765 | ID=Msa1367930-mRNA-1:exon:208;Parent=Msa1367930-mRNA-1 |
| tig0018573 | exon | 22438 | 22872 | 22438 | ID=Msa1367930-mRNA-1:exon:209;Parent=Msa1367930-mRNA-1 |
| tig0018573 | five_prime_UTR | 16629 | 16706 | 16629 | ID=Msa1367930-mRNA-1:five_prime_utr;Parent=Msa1367930-mRNA-1 |
| tig0018573 | CDS | 16707 | 16782 | 16707 | ID=Msa1367930-mRNA-1:cds;Parent=Msa1367930-mRNA-1 |
| tig0018573 | CDS | 17188 | 17728 | 17188 | ID=Msa1367930-mRNA-1:cds;Parent=Msa1367930-mRNA-1 |
| tig0018573 | CDS | 18133 | 18584 | 18133 | ID=Msa1367930-mRNA-1:cds;Parent=Msa1367930-mRNA-1 |
| tig0018573 | CDS | 18882 | 18999 | 18882 | ID=Msa1367930-mRNA-1:cds;Parent=Msa1367930-mRNA-1 |
| tig0018573 | CDS | 19534 | 19602 | 19534 | ID=Msa1367930-mRNA-1:cds;Parent=Msa1367930-mRNA-1 |
| tig0018573 | CDS | 19684 | 19752 | 19684 | ID=Msa1367930-mRNA-1:cds;Parent=Msa1367930-mRNA-1 |
| tig0018573 | CDS | 19864 | 19935 | 19864 | ID=Msa1367930-mRNA-1:cds;Parent=Msa1367930-mRNA-1 |
| tig0018573 | CDS | 20063 | 20131 | 20063 | ID=Msa1367930-mRNA-1:cds;Parent=Msa1367930-mRNA-1 |
| tig0018573 | CDS | 20346 | 20524 | 20346 | ID=Msa1367930-mRNA-1:cds;Parent=Msa1367930-mRNA-1 |
| tig0018573 | CDS | 20659 | 20864 | 20659 | ID=Msa1367930-mRNA-1:cds;Parent=Msa1367930-mRNA-1 |
| tig0018573 | CDS | 21104 | 21230 | 21104 | ID=Msa1367930-mRNA-1:cds;Parent=Msa1367930-mRNA-1 |
| tig0018573 | CDS | 21334 | 21682 | 21334 | ID=Msa1367930-mRNA-1:cds;Parent=Msa1367930-mRNA-1 |
| tig0018573 | CDS | 21765 | 21897 | 21765 | ID=Msa1367930-mRNA-1:cds;Parent=Msa1367930-mRNA-1 |
| tig0018573 | CDS | 22438 | 22656 | 22438 | ID=Msa1367930-mRNA-1:cds;Parent=Msa1367930-mRNA-1 |
| tig0018573 | three_prime_UTR | 22657 | 22872 | 22657 | ID=Msa1367930-mRNA-1:three_prime_utr;Parent=Msa1367930-mRNA-1 |
| Gene Sequence |
>Msa1367930 ATGGATTTAAGAGATTCATGGACTATTTTCTTTCTGCTTCATAGCTTTTTGGTTCTTGCTTTCTCTGGACAAGATGGTTTCTTGAGTCTATCATGTGGTGGAACAACAAGCTTCAATGATTCATCAAACATTTCATGGTTCTCAGACACTCCTTACATAACCATAGGAAAGACAGCCACCATCAATTACAATGATGGTTCCTTGTCAACAAATGTCTCGGCTCGGTTCTTCCCTCATTCCAAACGTCGCGCATGCTATCGGATACCAATGAACAATGCCACTAGTTTGATTCTTGTTAGAGCAAAATTTGTTTACAAGAACTATGACGGACTTGGAAAACCGCCTATTTTCTATGTTTCTCTCGGAACAGCTATTGCTGCTAAGATAAATCTAGCTAAAAAGGATCCATGGATAGAAGAGTTTCTCTGGGAAGTCAATAAGGATACATTGCCATTCTGTTTGAACTCAATTCCAAGTGGTGGTTCTCCTATAATTTCTTTACTTGAAATTAGACCTCTTCCTAAAGGTTCCTACAAAAAGGGCAAGGAAGATTTTCCTAATAAATTACTTAGGATGAGTTATAGAATTGATTGTGGACATATCAACGAGTCTATAAGGTATCCTATGGATCCATATGACAGAATATGGAACAGTGATAGAAGTTTCATACCTTTTCATGCTACAAGTGGGTTTAAGATTCAGAATAGCTTCAATCAATCCGATATTTTAGAGAAACCACCGGCTTCTGTTCTTCAAACAGGAAGAGTCTTGGCGCGACGAAATATAATGGCGTATAACCTACCTCTTGACGGGTTAGGGGACTACTATATTATCCTCTATTTTGCTGGAATTCTTCCTGTTTTCCCTTCGTTTGATGTATTCATAAATGGAGATCTTGTAAAGTCAAACTATACAATAAAAAGATCAGAAATCGGTGCTTTATACGTTACACGAAAGAGGATCAGCAGCTTGAATATAACACTAAGAAGTATCAACTTTTACCCTCAGATCAATGCATTCGAGGTATATAATATGGTTGATATTCCACCTGAAGCCTCCTCAACCACAGTTTCAGCAATGCAGGTTATTCAGCAGTCCACTGGCTTGGATCTAGGATGGCAAGATGATCCATGCTCTCCTTTCCCATGGGATCACATAGATTGTGAAGGAAACTTAGTGATATCATTGGATTTGCACAACACATCACTTGCCGGTGAAATCCAGAATTTGGGTAGCTTGCAAAACCTTGCAAAACTGAACCTTAGTTTCAATCAGCTGACATCTTTTGGCGAAGAGCTGGAGAACTTGATTAGTCTTCAAATTTTAGACTTGCGAGATAATAGTCTCCGAGGAGTAGTGCCTGATAACTTGGGGGAGTTAGAAGACCTCCACTTACTGAATCTAGAAAACAACAAACTACAAGGTCCACTTCCACAGTCTTTGAACAAAGACACTATAGAGATCAGAACATCTGGAAATTTGTGTCTTACTTTTTCCACAACCACGTGCGATGATGCATCATCCGATCCTCCAGTTGTGGCACCACAACTGATTATAATTCCCAAAAAGAAGAACCATGGACAAAACCATCTAGCAATAATACTAGGCACAGTTGGAGGAGCTACACTTACCATTTTCCTGATATGGGAAGAAACTGATATGAGGAACTGGGGAGCAGAAAAAGTTTTTACCTACAAAGAAATCAAAGCAGCTACAAGCAATTTTAAGGAAATCATTGGAAGAGGAGGTTTTGGATCTGTTTACCTTGGAAAGCTTCCAAATGGAAAATCAGTTGCTGTCAAAGTTCGGTTCGATAAATCCCAACTTGGGGTTGATTCCTTTATCAATGAGATTCATCTCTTGTCGAAAATCCGTCATCAAAATCTTGTGTCTTTGGAAGGATTCTGTCATGAAGCAAAGCATCAGATATTGGTTTATGAATACTTGCCTGGTGGATCCCTAGCTGAGCACCTCCATGGTGCAAACAGCCATAAAAATCCCTTAAGCTGGATTCGAAGGTTGAAAATTGCTGTCGATGCAGCAAAAGGTACTGCTTTAAGAGGGTCTGTTTGGATTCGTTTATTTAAGCCTATATACCGGTATAAACTACAACTTAAGCTCACTTCACTTTGTCAAGGATTGGATTATTTGCATAATGGAAGTGAACCAAGAATCATACACCGCGATGTCAAGTGTAGTAACATCCTCTTGGACATGGATTTGAATGCCAAGGTCTGTGATTTTGGCCTCTCTAAGCAGGTAACCAAAGCAGATGCAACGCATGTCACCACTGTTGTCAAGGGAACTGCTGGCTATCTAGATCCAGAGTATTATTCCACTCAACAGCTTACTGAGAAAAGTGATGTATATAGTTTTGGAGTTGTTCTTTTGGAACTCATTTGTGGAAGAGAGCCATTGATTCACTCTGGGACTCCTGATTCATTCAATTTGGTTTTATGGGCCAAACCCTATTTGCAGGCTGGTGCATTCGAAGTTGTTGATGAAAACATACAGGGAACTTTTGATCTAGAAAGCATGAAGAAGGCGACACTCATTGCTGTGAAGTCAGTGGAAAGGGATGCATCACAGAGGCCGCCTATTGCCGAAGTATTAGCAGAACTTAAGGAAGCTTATGGTATTCAGCTCAGATTTCTTGAGTCTTGTCAAAATGAAAATTGA |
| Protein sequence |
>Msa1367930 MDLRDSWTIFFLLHSFLVLAFSGQDGFLSLSCGGTTSFNDSSNISWFSDTPYITIGKTATINYNDGSLSTNVSARFFPHSKRRACYRIPMNNATSLILVRAKFVYKNYDGLGKPPIFYVSLGTAIAAKINLAKKDPWIEEFLWEVNKDTLPFCLNSIPSGGSPIISLLEIRPLPKGSYKKGKEDFPNKLLRMSYRIDCGHINESIRYPMDPYDRIWNSDRSFIPFHATSGFKIQNSFNQSDILEKPPASVLQTGRVLARRNIMAYNLPLDGLGDYYIILYFAGILPVFPSFDVFINGDLVKSNYTIKRSEIGALYVTRKRISSLNITLRSINFYPQINAFEVYNMVDIPPEASSTTVSAMQVIQQSTGLDLGWQDDPCSPFPWDHIDCEGNLVISLDLHNTSLAGEIQNLGSLQNLAKLNLSFNQLTSFGEELENLISLQILDLRDNSLRGVVPDNLGELEDLHLLNLENNKLQGPLPQSLNKDTIEIRTSGNLCLTFSTTTCDDASSDPPVVAPQLIIIPKKKNHGQNHLAIILGTVGGATLTIFLIWEETDMRNWGAEKVFTYKEIKAATSNFKEIIGRGGFGSVYLGKLPNGKSVAVKVRFDKSQLGVDSFINEIHLLSKIRHQNLVSLEGFCHEAKHQILVYEYLPGGSLAEHLHGANSHKNPLSWIRRLKIAVDAAKGTALRGSVWIRLFKPIYRYKLQLKLTSLCQGLDYLHNGSEPRIIHRDVKCSNILLDMDLNAKVCDFGLSKQVTKADATHVTTVVKGTAGYLDPEYYSTQQLTEKSDVYSFGVVLLELICGREPLIHSGTPDSFNLVLWAKPYLQAGAFEVVDENIQGTFDLESMKKATLIAVKSVERDASQRPPIAEVLAELKEAYGIQLRFLESCQNEN* |
