Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1375210 | XP_003606120.1 | 98.571 | 70 | 1 | 0 | 4 | 73 | 1 | 70 | 5.57e-44 | 146 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1375210 | G7JIE8 | 98.571 | 70 | 1 | 0 | 4 | 73 | 1 | 70 | 2.66e-44 | 146 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0167700 | Msa1375210 | 0.837169 | 6.092366e-57 | -8.615850e-47 |
| Msa1145460 | Msa1375210 | 0.819259 | 1.275693e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1375210 | MtrunA17_Chr4g0026341 | 98.571 | 70 | 1 | 0 | 4 | 73 | 1 | 70 | 5.12e-48 | 146 |
| Msa1375210 | MtrunA17_Chr4g0056721 | 95.714 | 70 | 3 | 0 | 4 | 73 | 1 | 70 | 4.68e-46 | 141 |
| Msa1375210 | MtrunA17_Chr2g0321091 | 78.462 | 65 | 14 | 0 | 9 | 73 | 5 | 69 | 2.15e-34 | 111 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1375210 | AT4G21105.1 | 75.758 | 66 | 16 | 0 | 8 | 73 | 3 | 68 | 1.44e-33 | 110 |
| Msa1375210 | AT4G21105.3 | 75.758 | 66 | 16 | 0 | 8 | 73 | 3 | 68 | 1.44e-33 | 110 |
| Msa1375210 | AT4G21105.2 | 70.000 | 40 | 12 | 0 | 8 | 47 | 3 | 42 | 1.04e-15 | 64.3 |
Find 35 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTTATAACTTGAGTTAAAA+TGG | 0.289749 | tig0019645:-103478 | Msa1375210:three_prime_UTR |
| CGAACAGAACCTCCTCATTA+TGG | 0.325538 | tig0019645:-103576 | Msa1375210:three_prime_UTR |
| TATAATATGTCGCATGGAAT+AGG | 0.329548 | tig0019645:-103657 | Msa1375210:CDS |
| TAAACTGTTCCTTCTAGTTA+TGG | 0.355570 | tig0019645:-103380 | Msa1375210:three_prime_UTR |
| AGGGCCCTTTCAAGTGAGTA+TGG | 0.384581 | tig0019645:+103869 | None:intergenic |
| AACAGACGTAATCTTATCAT+AGG | 0.410999 | tig0019645:+103849 | None:intergenic |
| AATCAAGAACCATAACTAGA+AGG | 0.459313 | tig0019645:+103371 | None:intergenic |
| CAGAGTTTGACCACCATTGT+GGG | 0.474697 | tig0019645:-103500 | Msa1375210:three_prime_UTR |
| AGCTTCTCTCGTGGTCTGAA+TGG | 0.478089 | tig0019645:+103928 | None:intergenic |
| TATGCATTTGTTCAGGGACG+AGG | 0.478227 | tig0019645:-103684 | Msa1375210:intron |
| GACTTGACTATTTCTGATGC+AGG | 0.480831 | tig0019645:-103623 | Msa1375210:three_prime_UTR |
| GGTCTGAATGGTGCTTCTGT+TGG | 0.499882 | tig0019645:+103940 | None:intergenic |
| GAGTATGGTTACGCATATGC+TGG | 0.507736 | tig0019645:+103884 | None:intergenic |
| CGTAACCATACTCACTTGAA+AGG | 0.509853 | tig0019645:-103874 | Msa1375210:CDS |
| ACATTTCAACTGTCTCATAG+TGG | 0.524035 | tig0019645:-103294 | Msa1375210:three_prime_UTR |
| TGGTTCTGCCATCCTGCACA+AGG | 0.530946 | tig0019645:+103960 | None:intergenic |
| CAATTGATGACTCAAACTAC+TGG | 0.535565 | tig0019645:-103434 | Msa1375210:three_prime_UTR |
| GTCGCATGGAATAGGAAAGA+AGG | 0.540132 | tig0019645:-103649 | Msa1375210:CDS |
| CATAATGAGGAGGTTCTGTT+CGG | 0.543796 | tig0019645:+103577 | None:intergenic |
| GATAATTGTTGGCCTTGTGC+AGG | 0.559216 | tig0019645:-103972 | None:intergenic |
| GCAGAGTTTGACCACCATTG+TGG | 0.565633 | tig0019645:-103501 | Msa1375210:three_prime_UTR |
| GGGATCTATAATATGTCGCA+TGG | 0.571320 | tig0019645:-103663 | Msa1375210:CDS |
| ACAGACGTAATCTTATCATA+GGG | 0.596059 | tig0019645:+103850 | None:intergenic |
| GTAACCATACTCACTTGAAA+GGG | 0.598233 | tig0019645:-103873 | Msa1375210:CDS |
| ATGCATTTGTTCAGGGACGA+GGG | 0.613696 | tig0019645:-103683 | Msa1375210:intron |
| ATTGTTGGCCTTGTGCAGGA+TGG | 0.618822 | tig0019645:-103968 | None:intergenic |
| TTTCAACTGTCTCATAGTGG+TGG | 0.619470 | tig0019645:-103291 | None:intergenic |
| GAAGCTGCAGTCAAAGCAAG+TGG | 0.624309 | tig0019645:+103820 | None:intergenic |
| AAAATCTCGAGTCCATAATG+AGG | 0.630429 | tig0019645:+103564 | None:intergenic |
| ATCTCGAGTCCATAATGAGG+AGG | 0.632319 | tig0019645:+103567 | None:intergenic |
| CTCAAGTTATAAACCCACAA+TGG | 0.646319 | tig0019645:+103487 | None:intergenic |
| TTCTCAATAAGCTTCTCTCG+TGG | 0.647709 | tig0019645:+103919 | None:intergenic |
| TATGGTTCTTGATTTGCATG+CGG | 0.672326 | tig0019645:-103362 | Msa1375210:three_prime_UTR |
| TTGACTATTTCTGATGCAGG+AGG | 0.679686 | tig0019645:-103620 | Msa1375210:three_prime_UTR |
| AAGTTATAAACCCACAATGG+TGG | 0.687611 | tig0019645:+103490 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTATTAAGAGAAAAGAA+TGG | + | tig0019645:103720-103739 | None:intergenic | 15.0% |
| !!! | AAGTATTGTTTATAAGGATA+TGG | + | tig0019645:103546-103565 | None:intergenic | 20.0% |
| !!! | TGCATAAAGTATTGTTTATA+AGG | + | tig0019645:103552-103571 | None:intergenic | 20.0% |
| !! | GTTTATAACTTGAGTTAAAA+TGG | - | tig0019645:103772-103791 | Msa1375210:intron | 20.0% |
| !!! | AAACTACTGGTTTTTTATTT+GGG | - | tig0019645:103829-103848 | Msa1375210:CDS | 20.0% |
| ! | AATACTTTATGCATTTGTTC+AGG | - | tig0019645:103559-103578 | Msa1375210:three_prime_UTR | 25.0% |
| ! | ATACTTTATGCATTTGTTCA+GGG | - | tig0019645:103560-103579 | Msa1375210:three_prime_UTR | 25.0% |
| !!! | CAAACTACTGGTTTTTTATT+TGG | - | tig0019645:103828-103847 | Msa1375210:CDS | 25.0% |
| ACAGACGTAATCTTATCATA+GGG | + | tig0019645:103403-103422 | None:intergenic | 30.0% | |
| AACAGACGTAATCTTATCAT+AGG | + | tig0019645:103404-103423 | None:intergenic | 30.0% | |
| TATGGCTATAAAGAAACAAG+TGG | + | tig0019645:103528-103547 | None:intergenic | 30.0% | |
| TATAATATGTCGCATGGAAT+AGG | - | tig0019645:103593-103612 | Msa1375210:three_prime_UTR | 30.0% | |
| ! | TAAACTGTTCCTTCTAGTTA+TGG | - | tig0019645:103870-103889 | Msa1375210:CDS | 30.0% |
| AATCAAGAACCATAACTAGA+AGG | + | tig0019645:103882-103901 | None:intergenic | 30.0% | |
| GTAACCATACTCACTTGAAA+GGG | - | tig0019645:103377-103396 | Msa1375210:three_prime_UTR | 35.0% | |
| AAAATCTCGAGTCCATAATG+AGG | + | tig0019645:103689-103708 | None:intergenic | 35.0% | |
| AAGTTATAAACCCACAATGG+TGG | + | tig0019645:103763-103782 | None:intergenic | 35.0% | |
| CTCAAGTTATAAACCCACAA+TGG | + | tig0019645:103766-103785 | None:intergenic | 35.0% | |
| ! | CAATTGATGACTCAAACTAC+TGG | - | tig0019645:103816-103835 | Msa1375210:CDS | 35.0% |
| !! | TATGGTTCTTGATTTGCATG+CGG | - | tig0019645:103888-103907 | Msa1375210:CDS | 35.0% |
| ACATTTCAACTGTCTCATAG+TGG | - | tig0019645:103956-103975 | Msa1375210:CDS | 35.0% | |
| TTCTCAATAAGCTTCTCTCG+TGG | + | tig0019645:103334-103353 | None:intergenic | 40.0% | |
| CGTAACCATACTCACTTGAA+AGG | - | tig0019645:103376-103395 | Msa1375210:three_prime_UTR | 40.0% | |
| GGGATCTATAATATGTCGCA+TGG | - | tig0019645:103587-103606 | Msa1375210:three_prime_UTR | 40.0% | |
| !! | GACTTGACTATTTCTGATGC+AGG | - | tig0019645:103627-103646 | Msa1375210:exon | 40.0% |
| !! | TTGACTATTTCTGATGCAGG+AGG | - | tig0019645:103630-103649 | Msa1375210:exon | 40.0% |
| CATAATGAGGAGGTTCTGTT+CGG | + | tig0019645:103676-103695 | None:intergenic | 40.0% | |
| !! | TTGGGTCTCTTGCTTGTTTT+TGG | - | tig0019645:103847-103866 | Msa1375210:CDS | 40.0% |
| GAGTATGGTTACGCATATGC+TGG | + | tig0019645:103369-103388 | None:intergenic | 45.0% | |
| TATGCATTTGTTCAGGGACG+AGG | - | tig0019645:103566-103585 | Msa1375210:three_prime_UTR | 45.0% | |
| ATGCATTTGTTCAGGGACGA+GGG | - | tig0019645:103567-103586 | Msa1375210:three_prime_UTR | 45.0% | |
| GTCGCATGGAATAGGAAAGA+AGG | - | tig0019645:103601-103620 | Msa1375210:three_prime_UTR | 45.0% | |
| CGAACAGAACCTCCTCATTA+TGG | - | tig0019645:103674-103693 | Msa1375210:intron | 45.0% | |
| ATCTCGAGTCCATAATGAGG+AGG | + | tig0019645:103686-103705 | None:intergenic | 45.0% | |
| CAGAGTTTGACCACCATTGT+GGG | - | tig0019645:103750-103769 | Msa1375210:intron | 45.0% | |
| GGTCTGAATGGTGCTTCTGT+TGG | + | tig0019645:103313-103332 | None:intergenic | 50.0% | |
| AGCTTCTCTCGTGGTCTGAA+TGG | + | tig0019645:103325-103344 | None:intergenic | 50.0% | |
| ! | AGGGCCCTTTCAAGTGAGTA+TGG | + | tig0019645:103384-103403 | None:intergenic | 50.0% |
| GAAGCTGCAGTCAAAGCAAG+TGG | + | tig0019645:103433-103452 | None:intergenic | 50.0% | |
| GCAGAGTTTGACCACCATTG+TGG | - | tig0019645:103749-103768 | Msa1375210:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0019645 | gene | 103292 | 103980 | 103292 | ID=Msa1375210;Name=Msa1375210 |
| tig0019645 | mRNA | 103292 | 103980 | 103292 | ID=Msa1375210-mRNA-1;Parent=Msa1375210;Name=Msa1375210-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0|1|0.5|1|1|1|2|352|73 |
| tig0019645 | exon | 103292 | 103691 | 103292 | ID=Msa1375210-mRNA-1:exon:4411;Parent=Msa1375210-mRNA-1 |
| tig0019645 | exon | 103807 | 103980 | 103807 | ID=Msa1375210-mRNA-1:exon:4410;Parent=Msa1375210-mRNA-1 |
| tig0019645 | CDS | 103807 | 103980 | 103807 | ID=Msa1375210-mRNA-1:cds;Parent=Msa1375210-mRNA-1 |
| tig0019645 | CDS | 103644 | 103691 | 103644 | ID=Msa1375210-mRNA-1:cds;Parent=Msa1375210-mRNA-1 |
| tig0019645 | three_prime_UTR | 103292 | 103643 | 103292 | ID=Msa1375210-mRNA-1:three_prime_utr;Parent=Msa1375210-mRNA-1 |
| Gene Sequence |
| Protein sequence |
