Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1384680 | KEH28473.1 | 99.200 | 125 | 1 | 0 | 1 | 125 | 1 | 125 | 1.75e-83 | 250 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1384680 | sp|F4IWB3|SMR13_ARATH | 46.316 | 95 | 41 | 3 | 22 | 112 | 14 | 102 | 2.45e-13 | 64.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1384680 | A0A072UH85 | 99.200 | 125 | 1 | 0 | 1 | 125 | 1 | 125 | 8.37e-84 | 250 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1340200 | Msa1384680 | 0.806651 | 7.417201e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1384680 | MtrunA17_Chr5g0447241 | 99.200 | 125 | 1 | 0 | 1 | 125 | 1 | 125 | 1.61e-87 | 250 |
| Msa1384680 | MtrunA17_Chr3g0103421 | 48.544 | 103 | 47 | 2 | 17 | 116 | 43 | 142 | 2.04e-22 | 85.9 |
| Msa1384680 | MtrunA17_Chr1g0173641 | 43.137 | 102 | 47 | 5 | 25 | 118 | 6 | 104 | 4.80e-13 | 60.8 |
| Msa1384680 | MtrunA17_Chr7g0257801 | 42.857 | 70 | 39 | 1 | 44 | 112 | 34 | 103 | 5.92e-11 | 55.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1384680 | AT3G20898.1 | 46.316 | 95 | 41 | 3 | 22 | 112 | 14 | 102 | 2.49e-14 | 64.3 |
| Msa1384680 | AT1G51355.1 | 54.237 | 59 | 22 | 2 | 61 | 116 | 54 | 110 | 1.80e-12 | 60.1 |
Find 16 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTGGACATGTTGAAATTTC+TGG | 0.227215 | tig0020668:-111502 | None:intergenic |
| GAAAGCACCCTTTGTTTCTT+TGG | 0.256395 | tig0020668:-111529 | None:intergenic |
| TTCTTCTTTGTTGATGTTGT+TGG | 0.272265 | tig0020668:-111295 | None:intergenic |
| TCTCAAGAAATGGTCCCTTT+AGG | 0.327602 | tig0020668:+111262 | None:intergenic |
| ACCCTTTGTTTCTTTGGTGC+TGG | 0.345077 | tig0020668:-111523 | None:intergenic |
| CTCAAACATGGATTCTGTTA+AGG | 0.345078 | tig0020668:+111411 | Msa1384680:CDS |
| TTGGTGTTGTCTTTCCTAAA+GGG | 0.351429 | tig0020668:-111276 | None:intergenic |
| GTTGGTGTTGTCTTTCCTAA+AGG | 0.417857 | tig0020668:-111277 | None:intergenic |
| CTTTGTTTCTTTGGTGCTGG+TGG | 0.438245 | tig0020668:-111520 | None:intergenic |
| CTTAACAGAATCCATGTTTG+AGG | 0.489575 | tig0020668:-111410 | None:intergenic |
| AGTTTATGCTCAACGCCAAA+AGG | 0.512710 | tig0020668:+111463 | Msa1384680:CDS |
| TGAAAACTGTTCCTCAAACA+TGG | 0.521395 | tig0020668:+111399 | Msa1384680:CDS |
| AAGAAGAAGACAAGAACAAG+AGG | 0.523933 | tig0020668:+111310 | Msa1384680:CDS |
| GCATAAACTGTTACTTGTGA+TGG | 0.526078 | tig0020668:-111449 | None:intergenic |
| CACCAGCACCAAAGAAACAA+AGG | 0.581716 | tig0020668:+111521 | Msa1384680:CDS |
| ACCAGCACCAAAGAAACAAA+GGG | 0.591665 | tig0020668:+111522 | Msa1384680:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATCCGAAATTTTTTACCTTT+TGG | - | tig0020668:111481-111500 | None:intergenic | 25.0% |
| TTCTTCTTTGTTGATGTTGT+TGG | - | tig0020668:111298-111317 | None:intergenic | 30.0% | |
| CGCCAAAAGGTAAAAAATTT+CGG | + | tig0020668:111476-111495 | Msa1384680:CDS | 30.0% | |
| !! | TTGGTGTTGTCTTTCCTAAA+GGG | - | tig0020668:111279-111298 | None:intergenic | 35.0% |
| AAGAAGAAGACAAGAACAAG+AGG | + | tig0020668:111310-111329 | Msa1384680:CDS | 35.0% | |
| TGAAAACTGTTCCTCAAACA+TGG | + | tig0020668:111399-111418 | Msa1384680:CDS | 35.0% | |
| CTTAACAGAATCCATGTTTG+AGG | - | tig0020668:111413-111432 | None:intergenic | 35.0% | |
| CTCAAACATGGATTCTGTTA+AGG | + | tig0020668:111411-111430 | Msa1384680:CDS | 35.0% | |
| ! | GCATAAACTGTTACTTGTGA+TGG | - | tig0020668:111452-111471 | None:intergenic | 35.0% |
| CACAAAAATTTCTCCAAGTC+TGG | - | tig0020668:111589-111608 | None:intergenic | 35.0% | |
| !! | GTTGGTGTTGTCTTTCCTAA+AGG | - | tig0020668:111280-111299 | None:intergenic | 40.0% |
| ! | GTTTGAGGAACAGTTTTCAG+AGG | - | tig0020668:111398-111417 | None:intergenic | 40.0% |
| AGTTTATGCTCAACGCCAAA+AGG | + | tig0020668:111463-111482 | Msa1384680:CDS | 40.0% | |
| GGTGGACATGTTGAAATTTC+TGG | - | tig0020668:111505-111524 | None:intergenic | 40.0% | |
| ACCAGCACCAAAGAAACAAA+GGG | + | tig0020668:111522-111541 | Msa1384680:CDS | 40.0% | |
| !! | GAAAGCACCCTTTGTTTCTT+TGG | - | tig0020668:111532-111551 | None:intergenic | 40.0% |
| ! | ATTTTTCGCAACACCAGACT+TGG | + | tig0020668:111573-111592 | Msa1384680:CDS | 40.0% |
| !! | CTTTGTTTCTTTGGTGCTGG+TGG | - | tig0020668:111523-111542 | None:intergenic | 45.0% |
| CACCAGCACCAAAGAAACAA+AGG | + | tig0020668:111521-111540 | Msa1384680:CDS | 45.0% | |
| ! | ACCCTTTGTTTCTTTGGTGC+TGG | - | tig0020668:111526-111545 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0020668 | gene | 111271 | 111648 | 111271 | ID=Msa1384680;Name=Msa1384680 |
| tig0020668 | mRNA | 111271 | 111648 | 111271 | ID=Msa1384680-mRNA-1;Parent=Msa1384680;Name=Msa1384680-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|125 |
| tig0020668 | exon | 111271 | 111648 | 111271 | ID=Msa1384680-mRNA-1:exon:9131;Parent=Msa1384680-mRNA-1 |
| tig0020668 | CDS | 111271 | 111648 | 111271 | ID=Msa1384680-mRNA-1:cds;Parent=Msa1384680-mRNA-1 |
| Gene Sequence |
| Protein sequence |
