AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1389790

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1389790 MtrunA17_Chr1g0202461 94.872 312 15 1 1 311 77 388 0.0 515
Msa1389790 MtrunA17_Chr8g0348311 67.544 114 34 2 160 271 132 244 4.53e-46 159
Msa1389790 MtrunA17_Chr2g0293481 55.738 122 40 2 164 276 238 354 1.39e-37 139
Msa1389790 MtrunA17_Chr8g0387501 44.809 183 84 7 137 311 84 257 1.76e-35 129
Msa1389790 MtrunA17_Chr7g0270051 40.260 154 70 2 147 292 8 147 5.76e-32 119
Msa1389790 MtrunA17_Chr2g0289181 49.074 108 52 2 173 277 3 110 1.94e-31 117
Msa1389790 MtrunA17_Chr8g0379461 42.446 139 65 3 123 259 26 151 6.64e-28 108
Msa1389790 MtrunA17_Chr2g0284321 56.522 92 33 2 163 248 9 99 2.17e-27 106
Msa1389790 MtrunA17_Chr5g0401821 58.824 85 29 2 163 242 174 257 7.86e-26 103
Msa1389790 MtrunA17_Chr4g0053281 54.118 85 34 1 163 242 240 324 7.32e-25 102
Msa1389790 MtrunA17_Chr2g0319961 52.747 91 33 1 164 244 50 140 8.77e-25 100
Msa1389790 MtrunA17_Chr1g0184691 67.797 59 19 0 185 243 294 352 4.14e-23 97.8
Msa1389790 MtrunA17_Chr4g0071521 51.807 83 35 1 165 242 221 303 8.09e-23 96.3
Msa1389790 MtrunA17_Chr1g0147881 48.750 80 40 1 162 241 241 319 1.46e-21 93.2
Msa1389790 MtrunA17_Chr7g0228941 52.459 61 29 0 181 241 49 109 8.17e-20 83.6
Msa1389790 MtrunA17_Chr7g0218081 45.652 92 49 1 158 249 384 474 9.38e-20 89.4
Msa1389790 MtrunA17_Chr7g0218081 43.077 65 35 2 180 244 224 286 1.11e-11 65.5
Msa1389790 MtrunA17_Chr4g0074001 45.783 83 43 2 166 247 340 421 5.38e-19 87.4
Msa1389790 MtrunA17_Chr3g0139021 44.578 83 45 1 159 241 290 371 7.56e-19 86.3
Msa1389790 MtrunA17_Chr7g0249311 44.048 84 45 2 166 248 373 455 1.72e-17 83.2
Msa1389790 MtrunA17_Chr7g0249311 45.205 73 38 2 172 244 167 237 1.12e-14 74.7
Msa1389790 MtrunA17_Chr3g0099641 38.835 103 61 1 181 281 248 350 3.48e-17 81.6
Msa1389790 MtrunA17_Chr7g0238921 51.429 70 31 2 172 241 114 180 1.14e-16 79.0
Msa1389790 MtrunA17_Chr4g0042831 45.455 77 40 2 172 247 265 340 2.42e-16 79.0
Msa1389790 MtrunA17_Chr4g0042831 41.176 68 39 1 177 244 138 204 9.15e-12 65.1
Msa1389790 MtrunA17_Chr5g0418191 44.156 77 41 2 172 247 307 382 2.99e-16 79.0
Msa1389790 MtrunA17_Chr5g0418191 42.647 68 37 2 177 244 144 209 1.54e-11 64.7
Msa1389790 MtrunA17_Chr2g0302391 43.678 87 49 0 184 270 211 297 3.52e-16 78.6
Msa1389790 MtrunA17_Chr7g0270831 46.575 73 39 0 182 254 214 286 3.74e-16 78.6
Msa1389790 MtrunA17_Chr5g0447701 40.217 92 53 2 170 260 237 327 4.49e-16 78.2
Msa1389790 MtrunA17_Chr5g0447701 39.080 87 51 2 158 244 66 150 7.61e-12 65.5
Msa1389790 MtrunA17_Chr7g0263531 44.737 76 40 2 179 254 184 257 5.17e-16 78.2
Msa1389790 MtrunA17_Chr7g0263531 40.816 98 42 3 162 244 324 420 1.82e-15 76.6
Msa1389790 MtrunA17_Chr3g0079691 42.353 85 47 2 166 249 476 559 5.87e-16 78.6
Msa1389790 MtrunA17_Chr3g0079691 49.315 73 34 3 183 254 279 349 3.34e-13 70.1
Msa1389790 MtrunA17_Chr7g0249301 52.381 63 29 1 185 247 293 354 1.00e-15 77.0
Msa1389790 MtrunA17_Chr7g0249301 45.205 73 38 2 172 244 50 120 2.96e-15 75.5
Msa1389790 MtrunA17_Chr1g0194481 49.275 69 33 2 185 252 159 226 1.26e-15 76.3
Msa1389790 MtrunA17_Chr4g0004911 42.169 83 46 2 166 247 317 398 1.76e-15 76.6
Msa1389790 MtrunA17_Chr4g0004911 32.000 125 69 2 185 309 185 293 2.23e-13 70.5
Msa1389790 MtrunA17_Chr4g0051881 48.649 74 37 1 185 258 179 251 2.37e-15 75.5
Msa1389790 MtrunA17_Chr2g0322391 50.000 64 32 0 181 244 346 409 5.51e-15 75.5
Msa1389790 MtrunA17_Chr3g0106491 38.542 96 57 2 166 260 346 440 5.58e-15 75.1
Msa1389790 MtrunA17_Chr3g0106491 44.444 63 33 2 183 245 197 257 2.10e-11 64.3
Msa1389790 MtrunA17_Chr3g0101131 49.206 63 31 1 185 247 416 477 6.06e-15 75.1
Msa1389790 MtrunA17_Chr3g0101131 38.961 77 45 2 177 253 236 310 3.42e-11 63.9
Msa1389790 MtrunA17_Chr5g0445311 46.479 71 34 2 185 252 365 434 6.54e-15 75.1
Msa1389790 MtrunA17_Chr2g0288871 49.206 63 32 0 182 244 303 365 8.16e-15 74.7
Msa1389790 MtrunA17_Chr5g0443131 52.459 61 28 1 185 245 158 217 9.20e-15 73.6
Msa1389790 MtrunA17_Chr4g0001361 41.333 75 44 0 182 256 160 234 1.04e-14 73.6
Msa1389790 MtrunA17_Chr4g0025051 45.205 73 40 0 182 254 333 405 1.25e-14 74.3
Msa1389790 MtrunA17_Chr5g0418781 42.222 90 44 3 158 241 93 180 1.31e-14 72.8
Msa1389790 MtrunA17_Chr5g0432161 41.176 85 48 2 166 249 402 485 1.32e-14 74.3
Msa1389790 MtrunA17_Chr5g0432161 48.387 62 30 2 183 244 243 302 6.17e-12 66.2
Msa1389790 MtrunA17_Chr8g0383251 39.759 83 47 2 164 244 86 167 1.32e-14 70.9
Msa1389790 MtrunA17_Chr7g0243891 37.624 101 60 3 163 262 352 450 1.54e-14 73.9
Msa1389790 MtrunA17_Chr7g0243891 45.161 62 32 2 183 244 215 274 8.04e-12 65.9
Msa1389790 MtrunA17_Chr1g0150801 52.632 57 26 1 185 241 103 158 1.60e-14 70.1
Msa1389790 MtrunA17_Chr3g0136961 38.824 85 52 0 185 269 139 223 2.36e-14 72.0
Msa1389790 MtrunA17_Chr3g0126391 52.632 57 25 1 185 239 134 190 2.37e-14 72.8
Msa1389790 MtrunA17_Chr8g0387981 41.176 85 43 2 162 239 105 189 2.44e-14 72.8
Msa1389790 MtrunA17_Chr4g0051181 50.820 61 30 0 185 245 244 304 2.59e-14 73.2
Msa1389790 MtrunA17_Chr4g0075201 37.168 113 57 2 185 283 268 380 3.57e-14 72.8
Msa1389790 MtrunA17_Chr4g0025061 43.836 73 41 0 182 254 358 430 5.00e-14 72.4
Msa1389790 MtrunA17_Chr7g0270191 40.860 93 53 2 185 276 146 237 6.58e-14 70.9
Msa1389790 MtrunA17_Chr4g0068541 45.902 61 32 1 185 245 209 268 7.43e-14 71.6
Msa1389790 MtrunA17_Chr3g0129321 46.667 60 31 1 185 244 119 177 7.81e-14 69.3
Msa1389790 MtrunA17_Chr7g0216891 38.835 103 58 2 152 254 192 289 1.03e-13 71.6
Msa1389790 MtrunA17_Chr4g0060271 50.000 60 30 0 185 244 256 315 1.15e-13 71.6
Msa1389790 MtrunA17_Chr2g0294231 47.541 61 31 1 185 245 204 263 1.39e-13 70.5
Msa1389790 MtrunA17_Chr2g0333851 35.644 101 62 1 185 285 170 267 2.45e-13 69.3
Msa1389790 MtrunA17_Chr8g0335811 40.506 79 45 2 165 242 134 211 2.79e-13 68.2
Msa1389790 MtrunA17_Chr1g0150281 36.842 76 48 0 185 260 141 216 3.78e-13 68.6
Msa1389790 MtrunA17_Chr1g0189391 42.553 94 43 5 172 255 84 176 5.29e-13 68.6
Msa1389790 MtrunA17_Chr8g0347251 35.514 107 57 3 163 257 110 216 1.04e-12 67.8
Msa1389790 MtrunA17_Chr1g0150271 33.962 106 68 2 185 289 102 206 1.39e-12 66.6
Msa1389790 MtrunA17_Chr7g0275531 44.928 69 32 2 185 247 119 187 3.32e-12 66.2
Msa1389790 MtrunA17_Chr5g0447711 44.286 70 38 1 178 247 224 292 3.77e-12 65.9
Msa1389790 MtrunA17_Chr7g0224801 42.623 61 34 1 181 241 90 149 6.03e-12 63.2
Msa1389790 MtrunA17_Chr6g0485431 49.180 61 29 2 185 244 155 214 6.67e-12 65.5
Msa1389790 MtrunA17_Chr8g0383351 46.552 58 30 1 185 242 161 217 9.49e-12 63.9
Msa1389790 MtrunA17_Chr5g0428351 41.250 80 40 2 162 239 166 240 1.44e-11 64.3
Msa1389790 MtrunA17_Chr3g0129361 46.552 58 30 1 185 242 145 201 1.81e-11 62.8
Msa1389790 MtrunA17_Chr1g0188941 42.623 61 34 1 185 245 52 111 6.45e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1389790 AT5G52830.1 75.229 109 23 1 162 266 143 251 4.89e-53 177
Msa1389790 AT4G01250.1 40.000 265 87 8 1 257 1 201 1.22e-47 162
Msa1389790 AT4G23550.1 62.745 102 38 0 169 270 125 226 6.22e-39 139
Msa1389790 AT1G30650.1 46.067 178 67 5 156 305 177 353 2.94e-36 135
Msa1389790 AT2G34830.1 56.757 111 39 1 151 252 173 283 3.77e-36 135
Msa1389790 AT2G34830.2 56.757 111 39 1 151 252 182 292 3.91e-36 135
Msa1389790 AT5G45050.4 43.716 183 85 4 140 310 1003 1179 7.20e-33 129
Msa1389790 AT5G45050.1 43.716 183 85 4 140 310 1142 1318 7.37e-33 129
Msa1389790 AT5G45050.2 43.716 183 85 4 140 310 1114 1290 7.41e-33 129
Msa1389790 AT1G29280.1 65.278 72 20 1 184 255 74 140 1.01e-25 103
Msa1389790 AT4G24240.1 58.824 85 29 2 163 242 256 339 1.65e-25 104
Msa1389790 AT3G58710.1 58.537 82 26 1 184 257 71 152 1.74e-25 103
Msa1389790 AT3G58710.2 58.537 82 26 1 184 257 70 151 1.97e-25 102
Msa1389790 AT2G23320.1 52.381 84 36 1 163 242 215 298 6.36e-25 102
Msa1389790 AT5G28650.1 67.797 59 19 0 185 243 263 321 1.14e-23 99.4
Msa1389790 AT5G45260.1 49.505 101 47 3 161 258 1185 1284 1.26e-23 102
Msa1389790 AT3G04670.1 67.797 59 19 0 185 243 263 321 2.30e-23 98.6
Msa1389790 AT2G24570.1 51.220 82 38 1 162 241 219 300 3.19e-22 95.1
Msa1389790 AT4G31550.1 50.000 82 39 1 162 241 222 303 4.51e-22 94.7
Msa1389790 AT4G31550.2 48.780 82 40 1 162 241 221 302 1.60e-21 93.2
Msa1389790 AT2G30590.1 51.250 80 38 1 164 243 294 372 1.72e-20 91.3
Msa1389790 AT4G30935.1 36.364 132 79 3 168 295 314 444 2.66e-18 85.5
Msa1389790 AT2G04880.1 44.565 92 44 3 165 250 287 377 8.54e-18 84.0
Msa1389790 AT2G04880.2 44.565 92 44 3 165 250 263 353 9.28e-18 84.0
Msa1389790 AT4G26440.1 43.158 95 49 3 166 256 353 446 1.14e-16 80.9
Msa1389790 AT4G26440.1 47.059 85 38 4 173 256 171 249 6.01e-15 75.9
Msa1389790 AT3G01080.2 53.125 64 29 1 181 244 341 403 1.95e-16 79.7
Msa1389790 AT3G01080.2 42.308 78 43 2 180 257 201 276 1.07e-13 71.6
Msa1389790 AT3G01080.1 53.125 64 29 1 181 244 303 365 2.12e-16 79.7
Msa1389790 AT3G01080.1 42.308 78 43 2 180 257 163 238 1.12e-13 71.6
Msa1389790 AT5G56270.1 43.373 83 45 2 166 247 468 549 4.82e-16 79.0
Msa1389790 AT5G56270.1 48.333 60 29 2 185 244 274 331 4.31e-12 67.0
Msa1389790 AT4G04450.2 39.326 89 54 0 182 270 196 284 7.32e-16 78.2
Msa1389790 AT1G13960.1 41.176 85 48 2 161 244 385 468 7.42e-16 78.2
Msa1389790 AT1G13960.1 34.862 109 60 4 141 244 185 287 8.45e-13 68.9
Msa1389790 AT1G13960.2 44.595 74 39 2 172 244 243 315 9.76e-16 77.4
Msa1389790 AT1G13960.2 34.862 109 60 4 141 244 32 134 1.98e-12 67.4
Msa1389790 AT2G38470.1 39.091 110 62 3 172 277 349 457 1.07e-15 77.8
Msa1389790 AT2G38470.1 36.290 124 68 4 185 302 185 303 6.82e-13 69.3
Msa1389790 AT1G69310.4 43.333 90 43 3 158 241 116 203 1.10e-15 76.3
Msa1389790 AT1G69310.2 43.333 90 43 3 158 241 116 203 1.10e-15 76.3
Msa1389790 AT1G69310.1 43.333 90 43 3 158 241 116 203 1.10e-15 76.3
Msa1389790 AT4G04450.1 39.326 89 54 0 182 270 290 378 1.36e-15 77.4
Msa1389790 AT2G03340.1 43.038 79 43 2 172 249 402 479 1.69e-15 77.4
Msa1389790 AT2G03340.1 42.308 78 43 2 179 256 245 320 5.10e-13 69.7
Msa1389790 AT3G62340.1 35.115 131 80 3 163 289 95 224 2.18e-15 75.5
Msa1389790 AT5G07100.1 39.796 98 53 3 164 256 213 309 2.26e-15 75.9
Msa1389790 AT5G07100.1 41.176 68 39 1 177 244 110 176 1.67e-12 67.4
Msa1389790 AT5G07100.2 39.796 98 53 3 164 256 120 216 3.34e-15 73.6
Msa1389790 AT5G07100.2 41.176 68 39 1 177 244 17 83 1.02e-12 66.6
Msa1389790 AT5G07100.4 39.796 98 53 3 164 256 120 216 3.34e-15 73.6
Msa1389790 AT5G07100.4 41.176 68 39 1 177 244 17 83 1.02e-12 66.6
Msa1389790 AT5G07100.5 39.796 98 53 3 164 256 120 216 3.34e-15 73.6
Msa1389790 AT5G07100.5 41.176 68 39 1 177 244 17 83 1.02e-12 66.6
Msa1389790 AT5G07100.3 39.796 98 53 3 164 256 120 216 3.34e-15 73.6
Msa1389790 AT5G07100.3 41.176 68 39 1 177 244 17 83 1.02e-12 66.6
Msa1389790 AT2G21900.1 40.449 89 45 2 164 245 82 169 3.69e-15 73.2
Msa1389790 AT4G22070.4 49.206 63 32 0 182 244 295 357 4.17e-15 76.3
Msa1389790 AT4G22070.3 49.206 63 32 0 182 244 295 357 4.17e-15 76.3
Msa1389790 AT4G22070.2 49.206 63 32 0 182 244 295 357 4.17e-15 76.3
Msa1389790 AT4G22070.1 49.206 63 32 0 182 244 295 357 4.17e-15 76.3
Msa1389790 AT1G29860.1 43.590 78 40 2 185 262 137 210 4.42e-15 74.7
Msa1389790 AT2G40740.3 41.667 120 53 5 160 269 152 264 5.21e-15 74.7
Msa1389790 AT2G40740.1 41.667 120 53 5 160 269 143 255 5.23e-15 74.3
Msa1389790 AT1G68150.1 48.000 75 38 1 185 259 236 309 5.51e-15 75.1
Msa1389790 AT4G01720.1 53.333 60 28 0 185 244 240 299 5.71e-15 75.5
Msa1389790 AT2G37260.1 45.882 85 39 3 162 241 323 405 6.27e-15 75.5
Msa1389790 AT2G37260.1 41.935 93 52 2 178 270 159 249 1.69e-14 73.9
Msa1389790 AT5G26170.1 51.724 58 27 1 185 242 114 170 6.33e-15 72.0
Msa1389790 AT2G37260.3 45.882 85 39 3 162 241 243 325 7.14e-15 74.7
Msa1389790 AT2G37260.3 41.935 93 52 2 178 270 79 169 1.70e-14 73.6
Msa1389790 AT1G62300.1 49.206 63 32 0 182 244 310 372 7.31e-15 75.5
Msa1389790 AT2G37260.2 45.882 85 39 3 162 241 241 323 7.53e-15 74.7
Msa1389790 AT2G37260.2 41.935 93 52 2 178 270 77 167 2.00e-14 73.2
Msa1389790 AT5G46350.1 47.297 74 38 1 185 258 184 256 7.96e-15 74.3
Msa1389790 AT5G49520.1 50.769 65 30 2 180 244 218 280 1.26e-14 74.3
Msa1389790 AT5G43290.1 39.560 91 54 1 151 241 81 170 1.48e-14 72.8
Msa1389790 AT5G15130.1 45.714 70 38 0 185 254 228 297 1.48e-14 74.3
Msa1389790 AT4G18170.1 51.613 62 29 1 185 246 173 233 1.51e-14 73.6
Msa1389790 AT5G15130.2 45.714 70 38 0 185 254 186 255 1.62e-14 74.3
Msa1389790 AT3G01080.3 42.308 78 43 2 180 257 201 276 6.27e-14 71.6
Msa1389790 AT4G26640.7 43.478 69 38 1 181 249 338 405 7.12e-14 72.4
Msa1389790 AT4G23810.1 35.115 131 65 5 185 310 159 274 7.39e-14 71.6
Msa1389790 AT4G26640.2 43.478 69 38 1 181 249 378 445 7.88e-14 72.4
Msa1389790 AT4G26640.1 43.478 69 38 1 181 249 306 373 9.28e-14 72.0
Msa1389790 AT1G69810.1 47.887 71 36 1 185 254 204 274 9.35e-14 71.6
Msa1389790 AT4G26640.5 43.478 69 38 1 181 249 202 269 1.02e-13 71.6
Msa1389790 AT4G26640.6 43.478 69 38 1 181 249 202 269 1.02e-13 71.6
Msa1389790 AT4G26640.4 43.478 69 38 1 181 249 202 269 1.02e-13 71.6
Msa1389790 AT4G26640.3 43.478 69 38 1 181 249 202 269 1.02e-13 71.6
Msa1389790 AT1G80840.1 39.437 71 43 0 185 255 147 217 1.14e-13 70.9
Msa1389790 AT1G69810.2 47.887 71 36 1 185 254 146 216 1.17e-13 70.9
Msa1389790 AT2G30250.1 44.000 75 40 2 172 245 315 388 1.18e-13 71.2
Msa1389790 AT2G25000.2 46.667 60 32 0 185 244 104 163 2.18e-13 68.9
Msa1389790 AT2G25000.4 46.667 60 32 0 185 244 104 163 2.18e-13 68.9
Msa1389790 AT2G25000.3 46.667 60 32 0 185 244 126 185 3.36e-13 68.6
Msa1389790 AT2G44745.1 41.026 78 44 2 165 241 125 201 3.58e-13 68.2
Msa1389790 AT4G31800.2 41.558 77 45 0 185 261 112 188 3.87e-13 68.6
Msa1389790 AT1G18860.1 50.000 60 30 0 185 244 240 299 4.05e-13 70.1
Msa1389790 AT2G40750.1 34.426 122 65 5 183 290 151 271 4.54e-13 69.3
Msa1389790 AT1G55600.1 37.500 80 48 2 166 244 288 366 5.22e-13 69.7
Msa1389790 AT2G25000.1 46.667 60 32 0 185 244 147 206 5.29e-13 68.6
Msa1389790 AT5G64810.1 49.123 57 28 1 185 241 111 166 5.29e-13 67.0
Msa1389790 AT4G31800.1 41.558 77 45 0 185 261 177 253 5.92e-13 68.6
Msa1389790 AT2G46130.1 40.000 80 46 2 163 241 8 86 6.88e-13 64.7
Msa1389790 AT5G41570.1 39.744 78 45 2 165 241 78 154 8.61e-13 66.2
Msa1389790 AT1G64000.1 45.161 62 33 1 180 241 110 170 9.05e-13 66.6
Msa1389790 AT2G46400.1 33.333 117 72 3 129 239 45 161 9.09e-13 68.2
Msa1389790 AT2G47260.1 37.864 103 54 4 166 260 154 254 9.75e-13 68.2
Msa1389790 AT3G56400.1 41.026 78 39 3 164 239 105 177 1.46e-12 67.4
Msa1389790 AT3G01970.1 43.548 62 34 1 181 242 62 122 2.41e-12 63.9
Msa1389790 AT5G13080.1 42.623 61 34 1 181 241 64 123 2.63e-12 63.9
Msa1389790 AT4G11070.2 50.877 57 26 1 185 239 110 166 3.74e-12 66.2
Msa1389790 AT4G11070.1 50.877 57 26 1 185 239 142 198 4.09e-12 66.2
Msa1389790 AT5G24110.1 39.024 82 48 1 160 239 89 170 5.30e-12 65.9
Msa1389790 AT4G39410.1 42.667 75 42 1 185 259 224 297 1.08e-11 65.1

Find 95 sgRNAs with CRISPR-Local

Find 262 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAGTTTAAGAACAATATTT+AGG 0.223895 tig0021300:+68486 Msa1389790:three_prime_UTR
TGGATGTGGAGAACCTTTAA+TGG 0.267330 tig0021300:-67771 None:intergenic
TGCAATATGAGGAGGGGAAT+TGG 0.276980 tig0021300:-67241 None:intergenic
TCTTGAAGCTCATTGGTTAT+AGG 0.277524 tig0021300:-67150 None:intergenic
GTGTTTGAGGCTGGATGCTT+TGG 0.300180 tig0021300:-67434 None:intergenic
CTTTCGCGCGGTACAACCTT+TGG 0.306785 tig0021300:-68012 None:intergenic
ATAAATGTCACAAATGGTTT+TGG 0.307644 tig0021300:+68424 Msa1389790:three_prime_UTR
TTGTGGGCATGGCGAAAATA+TGG 0.311492 tig0021300:+67740 Msa1389790:CDS
TGTTTGAGGCTGGATGCTTT+GGG 0.315208 tig0021300:-67433 None:intergenic
GTTATAGGTTGCACTACATT+AGG 0.317458 tig0021300:-67135 None:intergenic
TCTTTAGCACCAAACTCTTT+TGG 0.322210 tig0021300:+68215 Msa1389790:CDS
ACCTTCCCTTGGTCATGTTT+AGG 0.323922 tig0021300:-68309 None:intergenic
GCAATATGAGGAGGGGAATT+GGG 0.331773 tig0021300:-67240 None:intergenic
TCTTCTTCACCAAAAGAGTT+TGG 0.338560 tig0021300:-68224 None:intergenic
TGTTCTTAAACTACTTCATT+TGG 0.344047 tig0021300:-68477 None:intergenic
CTACCACTTCCTCTTCCATT+TGG 0.346152 tig0021300:+67298 Msa1389790:CDS
TAGTTTAAGAACAATATTTA+GGG 0.348956 tig0021300:+68487 Msa1389790:three_prime_UTR
AAACACCTAGATCAAGAAAA+AGG 0.350761 tig0021300:+67451 Msa1389790:CDS
GGGTAAAAGATGTCAATAAT+TGG 0.354816 tig0021300:-67179 None:intergenic
AGGAAGTGGTAGGGACAAAA+TGG 0.358832 tig0021300:-67287 None:intergenic
TATAACAATGGCTGAGGATT+GGG 0.363678 tig0021300:+66969 Msa1389790:exon
TCTGTTGTTGTTGTAATTGT+TGG 0.364018 tig0021300:-67371 None:intergenic
TTGATCTAGGTGTTTGAGGC+TGG 0.368009 tig0021300:-67443 None:intergenic
ATTTAAAACTTAAATGCAAT+AGG 0.371007 tig0021300:+68572 Msa1389790:three_prime_UTR
TGATTGGCAGCTTCGAACTA+TGG 0.377700 tig0021300:-67001 None:intergenic
TTTGCAATTAGAAGAGGAAA+TGG 0.384615 tig0021300:-67082 None:intergenic
TTTCTTGATCTAGGTGTTTG+AGG 0.387303 tig0021300:-67447 None:intergenic
GTTGTTGTAATTGTTGGTTA+TGG 0.398557 tig0021300:-67365 None:intergenic
GTTTAGATCTTGAAGCTCAT+TGG 0.407230 tig0021300:-67157 None:intergenic
GTGGTCTTCAAACACTTTCA+CGG 0.411062 tig0021300:+68337 Msa1389790:three_prime_UTR
GGATGTGGAGAACCTTTAAT+GGG 0.413222 tig0021300:-67770 None:intergenic
TTCTGTTGTGGATGTATTGG+TGG 0.430239 tig0021300:-67025 None:intergenic
GAAGTTTCTTGCACAATATG+AGG 0.432485 tig0021300:-67321 None:intergenic
TGGATGTATTGGTGGCTGAT+TGG 0.440667 tig0021300:-67017 None:intergenic
ATGTTCACTGTGACATATAC+AGG 0.458166 tig0021300:+68065 Msa1389790:CDS
CATGACCAAGGGAAGGTACT+TGG 0.460900 tig0021300:+68315 Msa1389790:three_prime_UTR
TTGCACAATATGAGGCCAAA+TGG 0.465159 tig0021300:-67313 None:intergenic
TTATAACAATGGCTGAGGAT+TGG 0.466049 tig0021300:+66968 Msa1389790:exon
CAAGAAGAAGTTGAGCTGTT+GGG 0.466337 tig0021300:-68188 None:intergenic
CGGATGTGTCACCGGAGACT+TGG 0.472713 tig0021300:-68144 None:intergenic
AGACTTGGTTCGTGTACTTC+CGG 0.473267 tig0021300:-68129 None:intergenic
GCAAGAAGAAGTTGAGCTGT+TGG 0.473676 tig0021300:-68189 None:intergenic
ACTATTTAAATATATGCCTC+TGG 0.485474 tig0021300:-68539 None:intergenic
TGCATTTATAACAATGGCTG+AGG 0.489485 tig0021300:+66963 Msa1389790:exon
CTCATTTGCATTTATAACAA+TGG 0.490614 tig0021300:+66957 Msa1389790:exon
TTCTACTTTGCAATTAGAAG+AGG 0.491486 tig0021300:-67088 None:intergenic
AGAGGTTATCTGCGGTTACA+TGG 0.495635 tig0021300:-67709 None:intergenic
TAACCTCTCAACAGATTTGT+GGG 0.500103 tig0021300:+67724 Msa1389790:CDS
AAAAGAGTTTGGTGCTAAAG+AGG 0.510944 tig0021300:-68213 None:intergenic
AGGCCAAATGGAAGAGGAAG+TGG 0.511721 tig0021300:-67301 None:intergenic
TATTAGATAAATGTCACAAA+TGG 0.513172 tig0021300:+68418 Msa1389790:three_prime_UTR
TTCCGGCTAGCGAGTTCCGA+TGG 0.517215 tig0021300:-68112 None:intergenic
TCCCTTGGTCATGTTTAGGA+AGG 0.527622 tig0021300:-68305 None:intergenic
TTATATCTGTACCTTGGATG+TGG 0.528855 tig0021300:-67785 None:intergenic
ATAACCTCTCAACAGATTTG+TGG 0.535451 tig0021300:+67723 Msa1389790:CDS
CTAGTAGTAGTGGTGATGGT+GGG 0.535550 tig0021300:-67201 None:intergenic
ACCCATCGGAACTCGCTAGC+CGG 0.537516 tig0021300:+68110 Msa1389790:CDS
TCCGATGGGTAGGTCTTGCA+TGG 0.545684 tig0021300:-68097 None:intergenic
CCTCATATTGCAAAATACAT+TGG 0.547832 tig0021300:+67252 Msa1389790:CDS
TACTTATGGGATTGTTCCAG+AGG 0.557829 tig0021300:+68523 Msa1389790:three_prime_UTR
GACCACCAAGTACCTTCCCT+TGG 0.558663 tig0021300:-68320 None:intergenic
GAGGTGGTCGGATGTGTCAC+CGG 0.563562 tig0021300:-68152 None:intergenic
TAGTAGTAGTGGTGATGGTG+GGG 0.565045 tig0021300:-67200 None:intergenic
TTAGAAGAGGAAATGGAAGA+TGG 0.565399 tig0021300:-67075 None:intergenic
GTTTGAGGCTGGATGCTTTG+GGG 0.566369 tig0021300:-67432 None:intergenic
AATATGAGGCCAAATGGAAG+AGG 0.570788 tig0021300:-67307 None:intergenic
TGACTAGAAATTGAGGTGGT+CGG 0.577828 tig0021300:-68164 None:intergenic
GAATATGTTGAAGCTAGAAG+TGG 0.578885 tig0021300:-67343 None:intergenic
TCCGGCTAGCGAGTTCCGAT+GGG 0.583330 tig0021300:-68111 None:intergenic
AGTAGTAGTGGTGATGGTGG+GGG 0.585398 tig0021300:-67199 None:intergenic
TCTCAACAGATTTGTGGGCA+TGG 0.588913 tig0021300:+67729 Msa1389790:CDS
GAAATTGAAACTGATGATGA+TGG 0.590055 tig0021300:+68263 Msa1389790:CDS
GACCAAGGGAAGGTACTTGG+TGG 0.605286 tig0021300:+68318 Msa1389790:three_prime_UTR
GTTGGGTTGACTAGAAATTG+AGG 0.607818 tig0021300:-68171 None:intergenic
TTCCTTCCTAAACATGACCA+AGG 0.610457 tig0021300:+68303 Msa1389790:exon
AAGTACACGAACCAAGTCTC+CGG 0.613947 tig0021300:+68133 Msa1389790:CDS
TTAAAGGTTCTCCACATCCA+AGG 0.614226 tig0021300:+67774 Msa1389790:CDS
AAATGGAAGAGGAAGTGGTA+GGG 0.618377 tig0021300:-67296 None:intergenic
ACTATGACCACTAGTAGTAG+TGG 0.620104 tig0021300:-67211 None:intergenic
TATAGATGTAGCAGTTCCAA+AGG 0.623205 tig0021300:+67996 Msa1389790:CDS
CAAATGGAAGAGGAAGTGGT+AGG 0.623737 tig0021300:-67297 None:intergenic
ACTAGTAGTAGTGGTGATGG+TGG 0.627374 tig0021300:-67202 None:intergenic
CCTTTCAACTTGCTTTCGCG+CGG 0.627775 tig0021300:-68024 None:intergenic
ATCTGTTGAGAGGTTATCTG+CGG 0.627787 tig0021300:-67717 None:intergenic
GCTAGCGAGTTCCGATGGGT+AGG 0.628478 tig0021300:-68107 None:intergenic
CCGCGCGAAAGCAAGTTGAA+AGG 0.629161 tig0021300:+68024 Msa1389790:CDS
AGAAGAAGAGGACATCGACA+TGG 0.634163 tig0021300:+68241 Msa1389790:CDS
ACCATCACCACTACTACTAG+TGG 0.637150 tig0021300:+67204 Msa1389790:CDS
ACCACTAGTAGTAGTGGTGA+TGG 0.637486 tig0021300:-67205 None:intergenic
ATGCCCACAAATCTGTTGAG+AGG 0.641173 tig0021300:-67727 None:intergenic
TCCTTCCTAAACATGACCAA+GGG 0.654939 tig0021300:+68304 Msa1389790:exon
AGATGGTATAGTATTTGTCG+TGG 0.665737 tig0021300:-67058 None:intergenic
TCCTAAACATGACCAAGGGA+AGG 0.673965 tig0021300:+68308 Msa1389790:exon
ACCATGCAAGACCTACCCAT+CGG 0.674071 tig0021300:+68096 Msa1389790:CDS
GGGTTGACTAGAAATTGAGG+TGG 0.732424 tig0021300:-68168 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACTCTAATAAAAATTAATT+TGG + tig0021300:67806-67825 Msa1389790:intron 10.0%
!!! AATCAATGTTTTAAAATTTT+TGG - tig0021300:67876-67895 None:intergenic 10.0%
!! AACTCTAATAAAAATTAATT+TGG + tig0021300:67806-67825 Msa1389790:intron 10.0%
!!! AATCAATGTTTTAAAATTTT+TGG - tig0021300:67876-67895 None:intergenic 10.0%
!!! AATATCACAACTATTTTTTT+GGG - tig0021300:66924-66943 None:intergenic 15.0%
!!! TAATATCACAACTATTTTTT+TGG - tig0021300:66925-66944 None:intergenic 15.0%
!!! TCTTCATTTTGTATATATAA+AGG + tig0021300:68374-68393 Msa1389790:three_prime_UTR 15.0%
!!! TAAAGGTTATAAGAAATTTT+TGG + tig0021300:68391-68410 Msa1389790:three_prime_UTR 15.0%
!! TAGTTTAAGAACAATATTTA+GGG + tig0021300:68487-68506 Msa1389790:three_prime_UTR 15.0%
!!! ATTTAAAACTTAAATGCAAT+AGG + tig0021300:68572-68591 Msa1389790:three_prime_UTR 15.0%
!!! AATATCACAACTATTTTTTT+GGG - tig0021300:66924-66943 None:intergenic 15.0%
!!! TAATATCACAACTATTTTTT+TGG - tig0021300:66925-66944 None:intergenic 15.0%
!!! TCTTCATTTTGTATATATAA+AGG + tig0021300:68374-68393 Msa1389790:three_prime_UTR 15.0%
!!! TAAAGGTTATAAGAAATTTT+TGG + tig0021300:68391-68410 Msa1389790:three_prime_UTR 15.0%
!! TAGTTTAAGAACAATATTTA+GGG + tig0021300:68487-68506 Msa1389790:three_prime_UTR 15.0%
!!! ATTTAAAACTTAAATGCAAT+AGG + tig0021300:68572-68591 Msa1389790:three_prime_UTR 15.0%
!!! ATATCACAACTATTTTTTTG+GGG - tig0021300:66923-66942 None:intergenic 20.0%
!!! ATTTTTTTAGTATTATGCCA+TGG - tig0021300:67561-67580 None:intergenic 20.0%
!!! TAATACAACAAAAAAGTGTA+AGG - tig0021300:67965-67984 None:intergenic 20.0%
!!! CACTTTTTTGTTGTATTATT+AGG + tig0021300:67967-67986 Msa1389790:intron 20.0%
!! TATTAGATAAATGTCACAAA+TGG + tig0021300:68418-68437 Msa1389790:three_prime_UTR 20.0%
!! GTAGTTTAAGAACAATATTT+AGG + tig0021300:68486-68505 Msa1389790:three_prime_UTR 20.0%
!!! ACATTTTCTAATGTACTTAT+GGG + tig0021300:68510-68529 Msa1389790:three_prime_UTR 20.0%
!!! ATATCACAACTATTTTTTTG+GGG - tig0021300:66923-66942 None:intergenic 20.0%
!!! ATTTTTTTAGTATTATGCCA+TGG - tig0021300:67561-67580 None:intergenic 20.0%
!!! TAATACAACAAAAAAGTGTA+AGG - tig0021300:67965-67984 None:intergenic 20.0%
!!! CACTTTTTTGTTGTATTATT+AGG + tig0021300:67967-67986 Msa1389790:intron 20.0%
!! TATTAGATAAATGTCACAAA+TGG + tig0021300:68418-68437 Msa1389790:three_prime_UTR 20.0%
!! GTAGTTTAAGAACAATATTT+AGG + tig0021300:68486-68505 Msa1389790:three_prime_UTR 20.0%
!!! ACATTTTCTAATGTACTTAT+GGG + tig0021300:68510-68529 Msa1389790:three_prime_UTR 20.0%
! CTCATTTGCATTTATAACAA+TGG + tig0021300:66957-66976 Msa1389790:exon 25.0%
! ATGTACTATTATTTATGCCA+TGG - tig0021300:67608-67627 None:intergenic 25.0%
! TTTCTGTTGAATAGGAAAAA+AGG - tig0021300:67665-67684 None:intergenic 25.0%
!! ATAAATGTCACAAATGGTTT+TGG + tig0021300:68424-68443 Msa1389790:three_prime_UTR 25.0%
! TGTTCTTAAACTACTTCATT+TGG - tig0021300:68480-68499 None:intergenic 25.0%
!! GACATTTTCTAATGTACTTA+TGG + tig0021300:68509-68528 Msa1389790:three_prime_UTR 25.0%
! ACTATTTAAATATATGCCTC+TGG - tig0021300:68542-68561 None:intergenic 25.0%
! CTCATTTGCATTTATAACAA+TGG + tig0021300:66957-66976 Msa1389790:exon 25.0%
! ATGTACTATTATTTATGCCA+TGG - tig0021300:67608-67627 None:intergenic 25.0%
! TTTCTGTTGAATAGGAAAAA+AGG - tig0021300:67665-67684 None:intergenic 25.0%
!! ATAAATGTCACAAATGGTTT+TGG + tig0021300:68424-68443 Msa1389790:three_prime_UTR 25.0%
! TGTTCTTAAACTACTTCATT+TGG - tig0021300:68480-68499 None:intergenic 25.0%
!! GACATTTTCTAATGTACTTA+TGG + tig0021300:68509-68528 Msa1389790:three_prime_UTR 25.0%
! ACTATTTAAATATATGCCTC+TGG - tig0021300:68542-68561 None:intergenic 25.0%
! ATTTTCTGTTGTGGATGTAT+TGG - tig0021300:67031-67050 None:intergenic 30.0%
! GGTAAATGAATTTTCTGTTG+TGG - tig0021300:67040-67059 None:intergenic 30.0%
TTTGCAATTAGAAGAGGAAA+TGG - tig0021300:67085-67104 None:intergenic 30.0%
TTCTACTTTGCAATTAGAAG+AGG - tig0021300:67091-67110 None:intergenic 30.0%
GGGTAAAAGATGTCAATAAT+TGG - tig0021300:67182-67201 None:intergenic 30.0%
! TGTATTTTGCAATATGAGGA+GGG - tig0021300:67251-67270 None:intergenic 30.0%
! ATGTATTTTGCAATATGAGG+AGG - tig0021300:67252-67271 None:intergenic 30.0%
! CCAATGTATTTTGCAATATG+AGG - tig0021300:67255-67274 None:intergenic 30.0%
CCTCATATTGCAAAATACAT+TGG + tig0021300:67252-67271 Msa1389790:CDS 30.0%
! GTTGTTGTAATTGTTGGTTA+TGG - tig0021300:67368-67387 None:intergenic 30.0%
! TCTGTTGTTGTTGTAATTGT+TGG - tig0021300:67374-67393 None:intergenic 30.0%
AAACACCTAGATCAAGAAAA+AGG + tig0021300:67451-67470 Msa1389790:CDS 30.0%
! TACTACCTTTTTCTTGATCT+AGG - tig0021300:67459-67478 None:intergenic 30.0%
!! TTTTTAGTATTATGCCATGG+TGG - tig0021300:67558-67577 None:intergenic 30.0%
TACTATTATTTATGCCATGG+TGG - tig0021300:67605-67624 None:intergenic 30.0%
!! GTTGACTTTTTCTGTTGAAT+AGG - tig0021300:67673-67692 None:intergenic 30.0%
TTCAACAGAAAAAGTCAACA+AGG + tig0021300:67674-67693 Msa1389790:intron 30.0%
TCAACAGAAAAAGTCAACAA+GGG + tig0021300:67675-67694 Msa1389790:intron 30.0%
AAAAAGTCAACAAGGGAAAA+TGG + tig0021300:67682-67701 Msa1389790:CDS 30.0%
TATGGACAAAAACCCATTAA+AGG + tig0021300:67758-67777 Msa1389790:CDS 30.0%
TTAGAGTTATATCTGTACCT+TGG - tig0021300:67794-67813 None:intergenic 30.0%
TATTGAACCATCAATTTCTC+AGG + tig0021300:67927-67946 Msa1389790:intron 30.0%
AGTATTTCCTGAGAAATTGA+TGG - tig0021300:67937-67956 None:intergenic 30.0%
! GAAATTGAAACTGATGATGA+TGG + tig0021300:68263-68282 Msa1389790:CDS 30.0%
! ATTTTCTGTTGTGGATGTAT+TGG - tig0021300:67031-67050 None:intergenic 30.0%
! GGTAAATGAATTTTCTGTTG+TGG - tig0021300:67040-67059 None:intergenic 30.0%
TTTGCAATTAGAAGAGGAAA+TGG - tig0021300:67085-67104 None:intergenic 30.0%
TTCTACTTTGCAATTAGAAG+AGG - tig0021300:67091-67110 None:intergenic 30.0%
GGGTAAAAGATGTCAATAAT+TGG - tig0021300:67182-67201 None:intergenic 30.0%
! TGTATTTTGCAATATGAGGA+GGG - tig0021300:67251-67270 None:intergenic 30.0%
! ATGTATTTTGCAATATGAGG+AGG - tig0021300:67252-67271 None:intergenic 30.0%
! CCAATGTATTTTGCAATATG+AGG - tig0021300:67255-67274 None:intergenic 30.0%
CCTCATATTGCAAAATACAT+TGG + tig0021300:67252-67271 Msa1389790:CDS 30.0%
! GTTGTTGTAATTGTTGGTTA+TGG - tig0021300:67368-67387 None:intergenic 30.0%
! TCTGTTGTTGTTGTAATTGT+TGG - tig0021300:67374-67393 None:intergenic 30.0%
AAACACCTAGATCAAGAAAA+AGG + tig0021300:67451-67470 Msa1389790:CDS 30.0%
! TACTACCTTTTTCTTGATCT+AGG - tig0021300:67459-67478 None:intergenic 30.0%
!! TTTTTAGTATTATGCCATGG+TGG - tig0021300:67558-67577 None:intergenic 30.0%
TACTATTATTTATGCCATGG+TGG - tig0021300:67605-67624 None:intergenic 30.0%
!! GTTGACTTTTTCTGTTGAAT+AGG - tig0021300:67673-67692 None:intergenic 30.0%
TTCAACAGAAAAAGTCAACA+AGG + tig0021300:67674-67693 Msa1389790:intron 30.0%
TCAACAGAAAAAGTCAACAA+GGG + tig0021300:67675-67694 Msa1389790:intron 30.0%
AAAAAGTCAACAAGGGAAAA+TGG + tig0021300:67682-67701 Msa1389790:CDS 30.0%
TATGGACAAAAACCCATTAA+AGG + tig0021300:67758-67777 Msa1389790:CDS 30.0%
TTAGAGTTATATCTGTACCT+TGG - tig0021300:67794-67813 None:intergenic 30.0%
TATTGAACCATCAATTTCTC+AGG + tig0021300:67927-67946 Msa1389790:intron 30.0%
AGTATTTCCTGAGAAATTGA+TGG - tig0021300:67937-67956 None:intergenic 30.0%
! GAAATTGAAACTGATGATGA+TGG + tig0021300:68263-68282 Msa1389790:CDS 30.0%
!!! ACAACTATTTTTTTGGGGTG+TGG - tig0021300:66918-66937 None:intergenic 35.0%
TGCATTTATAACAATGGCTG+AGG + tig0021300:66963-66982 Msa1389790:exon 35.0%
TTATAACAATGGCTGAGGAT+TGG + tig0021300:66968-66987 Msa1389790:exon 35.0%
TATAACAATGGCTGAGGATT+GGG + tig0021300:66969-66988 Msa1389790:exon 35.0%
AGATGGTATAGTATTTGTCG+TGG - tig0021300:67061-67080 None:intergenic 35.0%
TTAGAAGAGGAAATGGAAGA+TGG - tig0021300:67078-67097 None:intergenic 35.0%
GTTATAGGTTGCACTACATT+AGG - tig0021300:67138-67157 None:intergenic 35.0%
! TCTTGAAGCTCATTGGTTAT+AGG - tig0021300:67153-67172 None:intergenic 35.0%
GTTTAGATCTTGAAGCTCAT+TGG - tig0021300:67160-67179 None:intergenic 35.0%
! GTATTTTGCAATATGAGGAG+GGG - tig0021300:67250-67269 None:intergenic 35.0%
GAAGTTTCTTGCACAATATG+AGG - tig0021300:67324-67343 None:intergenic 35.0%
GAATATGTTGAAGCTAGAAG+TGG - tig0021300:67346-67365 None:intergenic 35.0%
TTTCTTGATCTAGGTGTTTG+AGG - tig0021300:67450-67469 None:intergenic 35.0%
ATAACCTCTCAACAGATTTG+TGG + tig0021300:67723-67742 Msa1389790:CDS 35.0%
TAACCTCTCAACAGATTTGT+GGG + tig0021300:67724-67743 Msa1389790:CDS 35.0%
TTATATCTGTACCTTGGATG+TGG - tig0021300:67788-67807 None:intergenic 35.0%
AGACGTAGTGTCAAATCTTT+CGG - tig0021300:67850-67869 None:intergenic 35.0%
TATAGATGTAGCAGTTCCAA+AGG + tig0021300:67996-68015 Msa1389790:CDS 35.0%
ATGTTCACTGTGACATATAC+AGG + tig0021300:68065-68084 Msa1389790:CDS 35.0%
!! AAAAGAGTTTGGTGCTAAAG+AGG - tig0021300:68216-68235 None:intergenic 35.0%
! TCTTTAGCACCAAACTCTTT+TGG + tig0021300:68215-68234 Msa1389790:CDS 35.0%
TCTTCTTCACCAAAAGAGTT+TGG - tig0021300:68227-68246 None:intergenic 35.0%
!!! ACAACTATTTTTTTGGGGTG+TGG - tig0021300:66918-66937 None:intergenic 35.0%
TGCATTTATAACAATGGCTG+AGG + tig0021300:66963-66982 Msa1389790:exon 35.0%
TTATAACAATGGCTGAGGAT+TGG + tig0021300:66968-66987 Msa1389790:exon 35.0%
TATAACAATGGCTGAGGATT+GGG + tig0021300:66969-66988 Msa1389790:exon 35.0%
AGATGGTATAGTATTTGTCG+TGG - tig0021300:67061-67080 None:intergenic 35.0%
TTAGAAGAGGAAATGGAAGA+TGG - tig0021300:67078-67097 None:intergenic 35.0%
GTTATAGGTTGCACTACATT+AGG - tig0021300:67138-67157 None:intergenic 35.0%
! TCTTGAAGCTCATTGGTTAT+AGG - tig0021300:67153-67172 None:intergenic 35.0%
GTTTAGATCTTGAAGCTCAT+TGG - tig0021300:67160-67179 None:intergenic 35.0%
! GTATTTTGCAATATGAGGAG+GGG - tig0021300:67250-67269 None:intergenic 35.0%
GAAGTTTCTTGCACAATATG+AGG - tig0021300:67324-67343 None:intergenic 35.0%
GAATATGTTGAAGCTAGAAG+TGG - tig0021300:67346-67365 None:intergenic 35.0%
TTTCTTGATCTAGGTGTTTG+AGG - tig0021300:67450-67469 None:intergenic 35.0%
ATAACCTCTCAACAGATTTG+TGG + tig0021300:67723-67742 Msa1389790:CDS 35.0%
TAACCTCTCAACAGATTTGT+GGG + tig0021300:67724-67743 Msa1389790:CDS 35.0%
TTATATCTGTACCTTGGATG+TGG - tig0021300:67788-67807 None:intergenic 35.0%
AGACGTAGTGTCAAATCTTT+CGG - tig0021300:67850-67869 None:intergenic 35.0%
TATAGATGTAGCAGTTCCAA+AGG + tig0021300:67996-68015 Msa1389790:CDS 35.0%
ATGTTCACTGTGACATATAC+AGG + tig0021300:68065-68084 Msa1389790:CDS 35.0%
!! AAAAGAGTTTGGTGCTAAAG+AGG - tig0021300:68216-68235 None:intergenic 35.0%
! TCTTTAGCACCAAACTCTTT+TGG + tig0021300:68215-68234 Msa1389790:CDS 35.0%
TCTTCTTCACCAAAAGAGTT+TGG - tig0021300:68227-68246 None:intergenic 35.0%
TTCTGTTGTGGATGTATTGG+TGG - tig0021300:67028-67047 None:intergenic 40.0%
ACTATGACCACTAGTAGTAG+TGG - tig0021300:67214-67233 None:intergenic 40.0%
AAATGGAAGAGGAAGTGGTA+GGG - tig0021300:67299-67318 None:intergenic 40.0%
AATATGAGGCCAAATGGAAG+AGG - tig0021300:67310-67329 None:intergenic 40.0%
TTGCACAATATGAGGCCAAA+TGG - tig0021300:67316-67335 None:intergenic 40.0%
TTACATACACAAAGCCACCA+TGG + tig0021300:67541-67560 Msa1389790:intron 40.0%
TTACATACACAAAGCCACCA+TGG + tig0021300:67541-67560 Msa1389790:intron 40.0%
ATCTGTTGAGAGGTTATCTG+CGG - tig0021300:67720-67739 None:intergenic 40.0%
GGATGTGGAGAACCTTTAAT+GGG - tig0021300:67773-67792 None:intergenic 40.0%
TGGATGTGGAGAACCTTTAA+TGG - tig0021300:67774-67793 None:intergenic 40.0%
TTAAAGGTTCTCCACATCCA+AGG + tig0021300:67774-67793 Msa1389790:CDS 40.0%
TGACTAGAAATTGAGGTGGT+CGG - tig0021300:68167-68186 None:intergenic 40.0%
!! GTTGGGTTGACTAGAAATTG+AGG - tig0021300:68174-68193 None:intergenic 40.0%
CAAGAAGAAGTTGAGCTGTT+GGG - tig0021300:68191-68210 None:intergenic 40.0%
! CTCTTTTGGTGAAGAAGAAG+AGG + tig0021300:68229-68248 Msa1389790:CDS 40.0%
TTCCTTCCTAAACATGACCA+AGG + tig0021300:68303-68322 Msa1389790:exon 40.0%
TCCTTCCTAAACATGACCAA+GGG + tig0021300:68304-68323 Msa1389790:exon 40.0%
GTGGTCTTCAAACACTTTCA+CGG + tig0021300:68337-68356 Msa1389790:three_prime_UTR 40.0%
TACTTATGGGATTGTTCCAG+AGG + tig0021300:68523-68542 Msa1389790:three_prime_UTR 40.0%
TTCTGTTGTGGATGTATTGG+TGG - tig0021300:67028-67047 None:intergenic 40.0%
ACTATGACCACTAGTAGTAG+TGG - tig0021300:67214-67233 None:intergenic 40.0%
AAATGGAAGAGGAAGTGGTA+GGG - tig0021300:67299-67318 None:intergenic 40.0%
AATATGAGGCCAAATGGAAG+AGG - tig0021300:67310-67329 None:intergenic 40.0%
TTGCACAATATGAGGCCAAA+TGG - tig0021300:67316-67335 None:intergenic 40.0%
TTACATACACAAAGCCACCA+TGG + tig0021300:67541-67560 Msa1389790:intron 40.0%
TTACATACACAAAGCCACCA+TGG + tig0021300:67541-67560 Msa1389790:intron 40.0%
ATCTGTTGAGAGGTTATCTG+CGG - tig0021300:67720-67739 None:intergenic 40.0%
GGATGTGGAGAACCTTTAAT+GGG - tig0021300:67773-67792 None:intergenic 40.0%
TGGATGTGGAGAACCTTTAA+TGG - tig0021300:67774-67793 None:intergenic 40.0%
TTAAAGGTTCTCCACATCCA+AGG + tig0021300:67774-67793 Msa1389790:CDS 40.0%
TGACTAGAAATTGAGGTGGT+CGG - tig0021300:68167-68186 None:intergenic 40.0%
!! GTTGGGTTGACTAGAAATTG+AGG - tig0021300:68174-68193 None:intergenic 40.0%
CAAGAAGAAGTTGAGCTGTT+GGG - tig0021300:68191-68210 None:intergenic 40.0%
! CTCTTTTGGTGAAGAAGAAG+AGG + tig0021300:68229-68248 Msa1389790:CDS 40.0%
TTCCTTCCTAAACATGACCA+AGG + tig0021300:68303-68322 Msa1389790:exon 40.0%
TCCTTCCTAAACATGACCAA+GGG + tig0021300:68304-68323 Msa1389790:exon 40.0%
GTGGTCTTCAAACACTTTCA+CGG + tig0021300:68337-68356 Msa1389790:three_prime_UTR 40.0%
TACTTATGGGATTGTTCCAG+AGG + tig0021300:68523-68542 Msa1389790:three_prime_UTR 40.0%
TGATTGGCAGCTTCGAACTA+TGG - tig0021300:67004-67023 None:intergenic 45.0%
! TGGATGTATTGGTGGCTGAT+TGG - tig0021300:67020-67039 None:intergenic 45.0%
!! TAGTAGTAGTGGTGATGGTG+GGG - tig0021300:67203-67222 None:intergenic 45.0%
!! CTAGTAGTAGTGGTGATGGT+GGG - tig0021300:67204-67223 None:intergenic 45.0%
!! ACTAGTAGTAGTGGTGATGG+TGG - tig0021300:67205-67224 None:intergenic 45.0%
ACCATCACCACTACTACTAG+TGG + tig0021300:67204-67223 Msa1389790:CDS 45.0%
ACCACTAGTAGTAGTGGTGA+TGG - tig0021300:67208-67227 None:intergenic 45.0%
GCAATATGAGGAGGGGAATT+GGG - tig0021300:67243-67262 None:intergenic 45.0%
TGCAATATGAGGAGGGGAAT+TGG - tig0021300:67244-67263 None:intergenic 45.0%
AGGAAGTGGTAGGGACAAAA+TGG - tig0021300:67290-67309 None:intergenic 45.0%
CAAATGGAAGAGGAAGTGGT+AGG - tig0021300:67300-67319 None:intergenic 45.0%
CTACCACTTCCTCTTCCATT+TGG + tig0021300:67298-67317 Msa1389790:CDS 45.0%
! TGTTTGAGGCTGGATGCTTT+GGG - tig0021300:67436-67455 None:intergenic 45.0%
! TTGATCTAGGTGTTTGAGGC+TGG - tig0021300:67446-67465 None:intergenic 45.0%
AGAGGTTATCTGCGGTTACA+TGG - tig0021300:67712-67731 None:intergenic 45.0%
ATGCCCACAAATCTGTTGAG+AGG - tig0021300:67730-67749 None:intergenic 45.0%
TCTCAACAGATTTGTGGGCA+TGG + tig0021300:67729-67748 Msa1389790:CDS 45.0%
TTGTGGGCATGGCGAAAATA+TGG + tig0021300:67740-67759 Msa1389790:CDS 45.0%
! AGACTTGGTTCGTGTACTTC+CGG - tig0021300:68132-68151 None:intergenic 45.0%
AAGTACACGAACCAAGTCTC+CGG + tig0021300:68133-68152 Msa1389790:CDS 45.0%
GGGTTGACTAGAAATTGAGG+TGG - tig0021300:68171-68190 None:intergenic 45.0%
GCAAGAAGAAGTTGAGCTGT+TGG - tig0021300:68192-68211 None:intergenic 45.0%
AGAAGAAGAGGACATCGACA+TGG + tig0021300:68241-68260 Msa1389790:CDS 45.0%
TCCCTTGGTCATGTTTAGGA+AGG - tig0021300:68308-68327 None:intergenic 45.0%
TCCTAAACATGACCAAGGGA+AGG + tig0021300:68308-68327 Msa1389790:exon 45.0%
ACCTTCCCTTGGTCATGTTT+AGG - tig0021300:68312-68331 None:intergenic 45.0%
TGATTGGCAGCTTCGAACTA+TGG - tig0021300:67004-67023 None:intergenic 45.0%
! TGGATGTATTGGTGGCTGAT+TGG - tig0021300:67020-67039 None:intergenic 45.0%
!! TAGTAGTAGTGGTGATGGTG+GGG - tig0021300:67203-67222 None:intergenic 45.0%
!! CTAGTAGTAGTGGTGATGGT+GGG - tig0021300:67204-67223 None:intergenic 45.0%
!! ACTAGTAGTAGTGGTGATGG+TGG - tig0021300:67205-67224 None:intergenic 45.0%
ACCATCACCACTACTACTAG+TGG + tig0021300:67204-67223 Msa1389790:CDS 45.0%
ACCACTAGTAGTAGTGGTGA+TGG - tig0021300:67208-67227 None:intergenic 45.0%
GCAATATGAGGAGGGGAATT+GGG - tig0021300:67243-67262 None:intergenic 45.0%
TGCAATATGAGGAGGGGAAT+TGG - tig0021300:67244-67263 None:intergenic 45.0%
AGGAAGTGGTAGGGACAAAA+TGG - tig0021300:67290-67309 None:intergenic 45.0%
CAAATGGAAGAGGAAGTGGT+AGG - tig0021300:67300-67319 None:intergenic 45.0%
CTACCACTTCCTCTTCCATT+TGG + tig0021300:67298-67317 Msa1389790:CDS 45.0%
! TGTTTGAGGCTGGATGCTTT+GGG - tig0021300:67436-67455 None:intergenic 45.0%
! TTGATCTAGGTGTTTGAGGC+TGG - tig0021300:67446-67465 None:intergenic 45.0%
AGAGGTTATCTGCGGTTACA+TGG - tig0021300:67712-67731 None:intergenic 45.0%
ATGCCCACAAATCTGTTGAG+AGG - tig0021300:67730-67749 None:intergenic 45.0%
TCTCAACAGATTTGTGGGCA+TGG + tig0021300:67729-67748 Msa1389790:CDS 45.0%
TTGTGGGCATGGCGAAAATA+TGG + tig0021300:67740-67759 Msa1389790:CDS 45.0%
! AGACTTGGTTCGTGTACTTC+CGG - tig0021300:68132-68151 None:intergenic 45.0%
AAGTACACGAACCAAGTCTC+CGG + tig0021300:68133-68152 Msa1389790:CDS 45.0%
GGGTTGACTAGAAATTGAGG+TGG - tig0021300:68171-68190 None:intergenic 45.0%
GCAAGAAGAAGTTGAGCTGT+TGG - tig0021300:68192-68211 None:intergenic 45.0%
AGAAGAAGAGGACATCGACA+TGG + tig0021300:68241-68260 Msa1389790:CDS 45.0%
TCCCTTGGTCATGTTTAGGA+AGG - tig0021300:68308-68327 None:intergenic 45.0%
TCCTAAACATGACCAAGGGA+AGG + tig0021300:68308-68327 Msa1389790:exon 45.0%
ACCTTCCCTTGGTCATGTTT+AGG - tig0021300:68312-68331 None:intergenic 45.0%
!! AGTAGTAGTGGTGATGGTGG+GGG - tig0021300:67202-67221 None:intergenic 50.0%
AGGCCAAATGGAAGAGGAAG+TGG - tig0021300:67304-67323 None:intergenic 50.0%
! GTTTGAGGCTGGATGCTTTG+GGG - tig0021300:67435-67454 None:intergenic 50.0%
! GTGTTTGAGGCTGGATGCTT+TGG - tig0021300:67437-67456 None:intergenic 50.0%
! CCTTTCAACTTGCTTTCGCG+CGG - tig0021300:68027-68046 None:intergenic 50.0%
ACCATGCAAGACCTACCCAT+CGG + tig0021300:68096-68115 Msa1389790:CDS 50.0%
!! CATGACCAAGGGAAGGTACT+TGG + tig0021300:68315-68334 Msa1389790:three_prime_UTR 50.0%
!! AGTAGTAGTGGTGATGGTGG+GGG - tig0021300:67202-67221 None:intergenic 50.0%
AGGCCAAATGGAAGAGGAAG+TGG - tig0021300:67304-67323 None:intergenic 50.0%
! GTTTGAGGCTGGATGCTTTG+GGG - tig0021300:67435-67454 None:intergenic 50.0%
! GTGTTTGAGGCTGGATGCTT+TGG - tig0021300:67437-67456 None:intergenic 50.0%
! CCTTTCAACTTGCTTTCGCG+CGG - tig0021300:68027-68046 None:intergenic 50.0%
ACCATGCAAGACCTACCCAT+CGG + tig0021300:68096-68115 Msa1389790:CDS 50.0%
!! CATGACCAAGGGAAGGTACT+TGG + tig0021300:68315-68334 Msa1389790:three_prime_UTR 50.0%
CTTTCGCGCGGTACAACCTT+TGG - tig0021300:68015-68034 None:intergenic 55.0%
! CCGCGCGAAAGCAAGTTGAA+AGG + tig0021300:68024-68043 Msa1389790:CDS 55.0%
TCCGATGGGTAGGTCTTGCA+TGG - tig0021300:68100-68119 None:intergenic 55.0%
!! GACCAAGGGAAGGTACTTGG+TGG + tig0021300:68318-68337 Msa1389790:three_prime_UTR 55.0%
GACCACCAAGTACCTTCCCT+TGG - tig0021300:68323-68342 None:intergenic 55.0%
CTTTCGCGCGGTACAACCTT+TGG - tig0021300:68015-68034 None:intergenic 55.0%
! CCGCGCGAAAGCAAGTTGAA+AGG + tig0021300:68024-68043 Msa1389790:CDS 55.0%
TCCGATGGGTAGGTCTTGCA+TGG - tig0021300:68100-68119 None:intergenic 55.0%
!! GACCAAGGGAAGGTACTTGG+TGG + tig0021300:68318-68337 Msa1389790:three_prime_UTR 55.0%
GACCACCAAGTACCTTCCCT+TGG - tig0021300:68323-68342 None:intergenic 55.0%
GCTAGCGAGTTCCGATGGGT+AGG - tig0021300:68110-68129 None:intergenic 60.0%
ACCCATCGGAACTCGCTAGC+CGG + tig0021300:68110-68129 Msa1389790:CDS 60.0%
TCCGGCTAGCGAGTTCCGAT+GGG - tig0021300:68114-68133 None:intergenic 60.0%
TTCCGGCTAGCGAGTTCCGA+TGG - tig0021300:68115-68134 None:intergenic 60.0%
CGGATGTGTCACCGGAGACT+TGG - tig0021300:68147-68166 None:intergenic 60.0%
GAGGTGGTCGGATGTGTCAC+CGG - tig0021300:68155-68174 None:intergenic 60.0%
GCTAGCGAGTTCCGATGGGT+AGG - tig0021300:68110-68129 None:intergenic 60.0%
ACCCATCGGAACTCGCTAGC+CGG + tig0021300:68110-68129 Msa1389790:CDS 60.0%
TCCGGCTAGCGAGTTCCGAT+GGG - tig0021300:68114-68133 None:intergenic 60.0%
TTCCGGCTAGCGAGTTCCGA+TGG - tig0021300:68115-68134 None:intergenic 60.0%
CGGATGTGTCACCGGAGACT+TGG - tig0021300:68147-68166 None:intergenic 60.0%
GAGGTGGTCGGATGTGTCAC+CGG - tig0021300:68155-68174 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0021300 gene 66904 68610 66904 ID=Msa1389790;Name=Msa1389790
tig0021300 mRNA 66904 68610 66904 ID=Msa1389790-mRNA-1;Parent=Msa1389790;Name=Msa1389790-mRNA-1;_AED=0.07;_eAED=0.07;_QI=72|1|1|1|1|1|3|297|311
tig0021300 exon 66904 67472 66904 ID=Msa1389790-mRNA-1:exon:11785;Parent=Msa1389790-mRNA-1
tig0021300 exon 67682 67795 67682 ID=Msa1389790-mRNA-1:exon:11786;Parent=Msa1389790-mRNA-1
tig0021300 exon 67989 68610 67989 ID=Msa1389790-mRNA-1:exon:11787;Parent=Msa1389790-mRNA-1
tig0021300 five_prime_UTR 66904 66975 66904 ID=Msa1389790-mRNA-1:five_prime_utr;Parent=Msa1389790-mRNA-1
tig0021300 CDS 66976 67472 66976 ID=Msa1389790-mRNA-1:cds;Parent=Msa1389790-mRNA-1
tig0021300 CDS 67682 67795 67682 ID=Msa1389790-mRNA-1:cds;Parent=Msa1389790-mRNA-1
tig0021300 CDS 67989 68313 67989 ID=Msa1389790-mRNA-1:cds;Parent=Msa1389790-mRNA-1
tig0021300 three_prime_UTR 68314 68610 68314 ID=Msa1389790-mRNA-1:three_prime_utr;Parent=Msa1389790-mRNA-1
Gene Sequence

>Msa1389790

ATGGCTGAGGATTGGGATTTGTTTGCCATAGTTCGAAGCTGCCAATCAGCCACCAATACATCCACAACAGAAAATTCATTTACCACGACAAATACTATACCATCTTCCATTTCCTCTTCTAATTGCAAAGTAGAATTAGAATATGATGCATTTTCTTTTCCTAATGTAGTGCAACCTATAACCAATGAGCTTCAAGATCTAAACCAATTATTGACATCTTTTACCCCCACCATCACCACTACTACTAGTGGTCATAGTATCAATCCCAATTCCCCTCCTCATATTGCAAAATACATTGGACAACAACAAAACCATTTTGTCCCTACCACTTCCTCTTCCATTTGGCCTCATATTGTGCAAGAAACTTCCACTTCTAGCTTCAACATATTCCATAACCAACAATTACAACAACAACAGATTCAACTACAAGCAATTCAGAAACAAGAATTTCAAGTACCCCAAAGCATCCAGCCTCAAACACCTAGATCAAGAAAAAGAAAAAGTCAACAAGGGAAAATGGTGTGCCATGTAACCGCAGATAACCTCTCAACAGATTTGTGGGCATGGCGAAAATATGGACAAAAACCCATTAAAGGTTCTCCACATCCAAGGAACTACTATAGATGTAGCAGTTCCAAAGGTTGTACCGCGCGAAAGCAAGTTGAAAGGAGCAACACTGAAGCAGACATGTTCACTGTGACATATACAGGAGATCATAACCATGCAAGACCTACCCATCGGAACTCGCTAGCCGGAAGTACACGAACCAAGTCTCCGGTGACACATCCGACCACCTCAATTTCTAGTCAACCCAACAGCTCAACTTCTTCTTGCTCCTCTTTAGCACCAAACTCTTTTGGTGAAGAAGAAGAGGACATCGACATGGAAATTGAAACTGATGATGATGGTGAAGATAGTTGTGATATTCCTTCCTAA
Protein sequence

>Msa1389790

MAEDWDLFAIVRSCQSATNTSTTENSFTTTNTIPSSISSSNCKVELEYDAFSFPNVVQPITNELQDLNQLLTSFTPTITTTTSGHSINPNSPPHIAKYIGQQQNHFVPTTSSSIWPHIVQETSTSSFNIFHNQQLQQQQIQLQAIQKQEFQVPQSIQPQTPRSRKRKSQQGKMVCHVTADNLSTDLWAWRKYGQKPIKGSPHPRNYYRCSSSKGCTARKQVERSNTEADMFTVTYTGDHNHARPTHRNSLAGSTRTKSPVTHPTTSISSQPNSSTSSCSSLAPNSFGEEEEDIDMEIETDDDGEDSCDIPS*