AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1396590

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1396590 MtrunA17_Chr1g0213811 97.153 281 3 2 1 276 1 281 0.0 562
Msa1396590 MtrunA17_Chr5g0406051 52.444 225 91 4 55 271 67 283 8.25e-72 222
Msa1396590 MtrunA17_Chr3g0143381 52.607 211 82 3 71 270 70 273 3.01e-70 218
Msa1396590 MtrunA17_Chr8g0371221 53.110 209 86 3 71 271 68 272 1.09e-69 216
Msa1396590 MtrunA17_Chr8g0371281 48.444 225 96 4 55 271 59 271 5.49e-68 212
Msa1396590 MtrunA17_Chr8g0371251 49.519 208 93 3 71 270 69 272 2.57e-64 202
Msa1396590 MtrunA17_Chr6g0452281 48.558 208 90 3 73 270 54 254 6.28e-62 197
Msa1396590 MtrunA17_Chr4g0066531 47.418 213 96 3 69 270 36 243 1.18e-59 191
Msa1396590 MtrunA17_Chr8g0371231 51.282 195 83 3 85 271 7 197 2.39e-59 187
Msa1396590 MtrunA17_Chr2g0286811 45.370 216 100 3 68 270 38 248 1.39e-56 184
Msa1396590 MtrunA17_Chr3g0143361 49.398 166 82 1 105 270 4 167 2.63e-46 154
Msa1396590 MtrunA17_Chr4g0053801 37.712 236 136 4 41 272 62 290 6.83e-43 148
Msa1396590 MtrunA17_Chr5g0401481 35.094 265 145 6 31 275 50 307 7.58e-43 148
Msa1396590 MtrunA17_Chr8g0371241 44.000 175 68 4 106 271 4 157 1.82e-40 138
Msa1396590 MtrunA17_Chr3g0143371 47.403 154 78 2 102 254 1 152 5.28e-37 128
Msa1396590 MtrunA17_Chr3g0143351 44.970 169 84 3 105 270 4 166 4.48e-35 124
Msa1396590 MtrunA17_Chr6g0470551 30.189 265 153 7 8 270 4 238 3.15e-31 117
Msa1396590 MtrunA17_Chr3g0098671 53.448 58 26 1 154 211 31 87 4.02e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1396590 AT2G43080.1 69.892 279 72 2 4 272 3 279 1.26e-146 412
Msa1396590 AT1G20270.1 56.459 209 77 3 71 270 77 280 1.29e-72 224
Msa1396590 AT2G43080.2 60.773 181 59 2 4 174 3 181 5.57e-71 217
Msa1396590 AT4G35810.1 55.238 210 78 4 71 270 81 284 7.75e-71 220
Msa1396590 AT4G35810.2 55.238 210 78 4 71 270 81 284 7.75e-71 220
Msa1396590 AT5G66060.1 48.963 241 98 4 42 271 57 283 1.18e-69 217
Msa1396590 AT2G17720.1 52.857 210 83 4 71 270 81 284 9.11e-67 209
Msa1396590 AT3G28480.1 39.615 260 133 5 28 270 7 259 8.60e-58 187
Msa1396590 AT3G06300.1 46.698 212 99 3 69 270 37 244 7.43e-57 184
Msa1396590 AT3G28490.1 47.087 206 97 4 73 270 35 236 1.29e-56 184
Msa1396590 AT5G18900.1 45.070 213 101 3 69 270 36 243 7.49e-55 179
Msa1396590 AT3G28480.2 36.842 266 140 6 28 270 7 267 1.39e-47 161
Msa1396590 AT5G66060.2 50.588 170 73 3 42 211 57 215 6.88e-46 154
Msa1396590 AT1G20270.2 59.055 127 50 1 71 197 77 201 3.91e-43 146
Msa1396590 AT1G20270.3 59.055 127 50 1 71 197 77 201 3.91e-43 146
Msa1396590 AT4G35820.1 45.833 192 78 4 71 254 89 262 1.01e-42 147
Msa1396590 AT4G33910.1 38.428 229 130 4 47 271 61 282 2.79e-42 146
Msa1396590 AT2G23096.1 34.466 206 129 3 73 275 71 273 2.43e-34 125
Msa1396590 AT4G25600.1 29.752 242 135 7 39 270 21 237 2.48e-22 94.4
Msa1396590 AT4G25600.3 30.102 196 109 5 78 270 6 176 1.91e-18 82.4
Msa1396590 AT4G25600.4 30.102 196 109 5 78 270 3 173 1.92e-18 82.4
Msa1396590 AT4G25600.2 30.102 196 109 5 78 270 15 185 2.43e-18 82.4

Find 96 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTACAATGCTTATGTATTT+AGG 0.204792 tig0022040:-94162 Msa1396590:CDS
CTTGAAGTTGTTGTTGTAAA+TGG 0.219348 tig0022040:+94735 None:intergenic
GTCACAATCGGAATGATTAT+TGG 0.231053 tig0022040:-94888 Msa1396590:intron
ACGAGGAATGTGATTATCTT+AGG 0.235744 tig0022040:-94587 Msa1396590:CDS
AGTTGGTCTTAGTACTTAAT+TGG 0.245028 tig0022040:-92298 Msa1396590:three_prime_UTR
TCTCAAATTCCAATTGAAAA+TGG 0.255513 tig0022040:-94405 Msa1396590:CDS
TTGGATAAGCAGCTAAATTA+AGG 0.273419 tig0022040:-92227 Msa1396590:three_prime_UTR
AGCACAGCATTTCCTTTAAC+TGG 0.282461 tig0022040:+92834 None:intergenic
GGAGGATGCCCGGTACTTGC+TGG 0.290409 tig0022040:-92635 Msa1396590:CDS
GTCGTCGTTATTATAACCTC+TGG 0.331910 tig0022040:+94701 None:intergenic
TTTCTGATCAAGGCTTTGTT+TGG 0.366792 tig0022040:-92246 Msa1396590:three_prime_UTR
TTCAGCTCCATTTCTGATCA+AGG 0.388513 tig0022040:-92256 Msa1396590:three_prime_UTR
TTATGTGTGAAACCAGTTAA+AGG 0.389095 tig0022040:-92846 Msa1396590:CDS
AACAAAGCCTTGATCAGAAA+TGG 0.390787 tig0022040:+92249 None:intergenic
CGTATGATTTAGCTAGCTTT+CGG 0.397475 tig0022040:-92570 Msa1396590:exon
AGTGATGTCAGAACAAGTTC+TGG 0.417022 tig0022040:-94498 Msa1396590:CDS
GGAGACCAACTAAGGACTTC+AGG 0.418550 tig0022040:+94642 None:intergenic
CGAGGAATGTGATTATCTTA+GGG 0.422123 tig0022040:-94586 Msa1396590:CDS
TGCAGGTGCTCTCTCTCAAT+TGG 0.426323 tig0022040:-94787 Msa1396590:intron
TGTTATTTCTATTTAAAATG+AGG 0.430096 tig0022040:-92166 Msa1396590:three_prime_UTR
CTTATTTCCACAACTTCTCT+AGG 0.430854 tig0022040:+92113 None:intergenic
CTTCTCACTTTCGTCACAAT+CGG 0.443635 tig0022040:-94900 Msa1396590:CDS
GATGGTTTGATCTATATCAT+TGG 0.448253 tig0022040:-92390 Msa1396590:three_prime_UTR
TCGTCGTTATTATAACCTCT+GGG 0.448332 tig0022040:+94702 None:intergenic
TGGTTTCACACATAACCCTT+TGG 0.454448 tig0022040:+92854 None:intergenic
CATCCTCCATGCACACTATC+CGG 0.458292 tig0022040:+92650 None:intergenic
CATTTATTAACATGGATCCT+TGG 0.458502 tig0022040:-95047 Msa1396590:five_prime_UTR
CACTTCTCCCCAGCAAGTAC+CGG 0.463904 tig0022040:+92626 None:intergenic
GAGGATGCCCGGTACTTGCT+GGG 0.470338 tig0022040:-92634 Msa1396590:CDS
TATTTAGGTGACAATGTTGA+GGG 0.478530 tig0022040:-94147 Msa1396590:CDS
AAAGAAAGAGATTGACTGAC+TGG 0.485358 tig0022040:-94979 Msa1396590:five_prime_UTR
TTAGCTAGCTTTCGGTGGGT+AGG 0.486072 tig0022040:-92562 Msa1396590:three_prime_UTR
TTAAACCTGAAGTCCTTAGT+TGG 0.490970 tig0022040:-94647 Msa1396590:CDS
TCATGTGACCTTGTCATAGT+TGG 0.497920 tig0022040:-92315 Msa1396590:three_prime_UTR
GTCGATGCAAACACAGGAAA+GGG 0.501803 tig0022040:-94528 Msa1396590:CDS
TAGCTAGCTTTCGGTGGGTA+GGG 0.504284 tig0022040:-92561 Msa1396590:three_prime_UTR
GAAGCACAAATCTTACGCCT+TGG 0.505360 tig0022040:-94675 Msa1396590:CDS
CCGGCGATGAAGATTGTGTT+CGG 0.507131 tig0022040:-94924 Msa1396590:CDS
AGGTGACAATGTTGAGGGAG+GGG 0.507663 tig0022040:-94142 Msa1396590:CDS
GAGAGCTTATGCAAGTCCTT+AGG 0.507911 tig0022040:-94383 Msa1396590:CDS
TTATGAAGTAAAATAATTCG+TGG 0.516165 tig0022040:+94621 None:intergenic
TAAAGATATATCCTTGCGAT+TGG 0.518385 tig0022040:-92357 Msa1396590:three_prime_UTR
TGATATTAATACACAATGGA+TGG 0.527767 tig0022040:-92408 Msa1396590:three_prime_UTR
TGGTATTATCAGGGACTGGA+TGG 0.528484 tig0022040:-92683 Msa1396590:intron
AGTTTCGATCAATAAAATAC+AGG 0.529356 tig0022040:-92439 Msa1396590:three_prime_UTR
TAGGTGACAATGTTGAGGGA+GGG 0.530460 tig0022040:-94143 Msa1396590:CDS
ATGATTTAGCTAGCTTTCGG+TGG 0.531891 tig0022040:-92567 Msa1396590:exon
ACTGGATGGACAGTCAGATC+CGG 0.531985 tig0022040:-92669 Msa1396590:CDS
ACTTTGAAGTGAAAATATGA+AGG 0.535155 tig0022040:+92511 None:intergenic
CGGTACTTGCTGGGGAGAAG+TGG 0.535804 tig0022040:-92625 Msa1396590:CDS
ATGGTTTGATCTATATCATT+GGG 0.540700 tig0022040:-92389 Msa1396590:three_prime_UTR
TAGTGTGCATGGAGGATGCC+CGG 0.552060 tig0022040:-92645 Msa1396590:CDS
TTAGGTGACAATGTTGAGGG+AGG 0.555757 tig0022040:-94144 Msa1396590:CDS
GTATTTAGGTGACAATGTTG+AGG 0.557593 tig0022040:-94148 Msa1396590:CDS
CTTTGAAGTGAAAATATGAA+GGG 0.561377 tig0022040:+92512 None:intergenic
GTGGTGGGAAATTAACCAAA+GGG 0.569757 tig0022040:-92869 Msa1396590:CDS
AATCGAATCGATGAGTCCTC+CGG 0.571781 tig0022040:-94943 Msa1396590:exon
TAAGCATTGTAGCTATTCGC+TGG 0.573513 tig0022040:+94172 None:intergenic
TAATTCGTGGAGACCAACTA+AGG 0.573940 tig0022040:+94634 None:intergenic
CGTCGATGCAAACACAGGAA+AGG 0.581169 tig0022040:-94529 Msa1396590:CDS
AGAGAAGACATTTATTAACA+TGG 0.582619 tig0022040:-95055 Msa1396590:five_prime_UTR
ACTGATTCTTCTCATACCTA+AGG 0.583409 tig0022040:+94367 None:intergenic
TCAGATCCGGATAGTGTGCA+TGG 0.584101 tig0022040:-92656 Msa1396590:CDS
ACTTCTCCCCAGCAAGTACC+GGG 0.590424 tig0022040:+92627 None:intergenic
CCGAACACAATCTTCATCGC+CGG 0.591398 tig0022040:+94924 None:intergenic
GGTCGGCTACAAAGTGGATG+AGG 0.597597 tig0022040:-92604 Msa1396590:CDS
CTTCAGGTTTAACATATCCA+AGG 0.599046 tig0022040:+94658 None:intergenic
CAGTTCAATTTGAAGCGTGG+TGG 0.599421 tig0022040:-94195 Msa1396590:CDS
ATATGAAGGGGAAAACCCCT+TGG 0.600022 tig0022040:+92525 None:intergenic
ATAGTTTAAATATTAAATAG+CGG 0.603042 tig0022040:-92464 Msa1396590:three_prime_UTR
AAGCTATCCTAGAGAAGTTG+TGG 0.604760 tig0022040:-92120 Msa1396590:three_prime_UTR
AATCACATTCCTCGTAGCTG+AGG 0.604915 tig0022040:+94595 None:intergenic
TGATTTAGCTAGCTTTCGGT+GGG 0.607907 tig0022040:-92566 Msa1396590:three_prime_UTR
TCAGATGAATGTAGCTGTGG+TGG 0.613560 tig0022040:-92885 Msa1396590:CDS
AGGATGCCCGGTACTTGCTG+GGG 0.623094 tig0022040:-92633 Msa1396590:CDS
AAATACTGAGACCAATCGCA+AGG 0.629731 tig0022040:+92346 None:intergenic
AACACAATCTTCATCGCCGG+AGG 0.630657 tig0022040:+94927 None:intergenic
AGAAGTGGTCGGCTACAAAG+TGG 0.632725 tig0022040:-92610 Msa1396590:CDS
GAGGAATGTGATTATCTTAG+GGG 0.634805 tig0022040:-94585 Msa1396590:CDS
TAACAGTTCAATTTGAAGCG+TGG 0.636945 tig0022040:-94198 Msa1396590:intron
TGTGGTGGGAAATTAACCAA+AGG 0.637747 tig0022040:-92870 Msa1396590:CDS
CAGATGAATGTAGCTGTGGT+GGG 0.637801 tig0022040:-92884 Msa1396590:CDS
GGTTCAGATGAATGTAGCTG+TGG 0.641033 tig0022040:-92888 Msa1396590:CDS
TTTGAAGTGAAAATATGAAG+GGG 0.641588 tig0022040:+92513 None:intergenic
ACTTGCTGGGGAGAAGTGGT+CGG 0.648554 tig0022040:-92621 Msa1396590:CDS
AGGGGAAACGCATTTCCCAT+CGG 0.656658 tig0022040:-94124 Msa1396590:intron
AACTTCTCTAGGATAGCTTG+CGG 0.661308 tig0022040:+92124 None:intergenic
TGTATGATATTAATACACAA+TGG 0.662040 tig0022040:-92412 Msa1396590:three_prime_UTR
ACTGTCGTCGATGCAAACAC+AGG 0.669566 tig0022040:-94534 Msa1396590:CDS
AAATGGAGCTGAAATCTCAA+CGG 0.670043 tig0022040:+92266 None:intergenic
CGTCGTTATTATAACCTCTG+GGG 0.670331 tig0022040:+94703 None:intergenic
TACTAAGACCAACTATGACA+AGG 0.677316 tig0022040:+92307 None:intergenic
TGGAGCTGAAATCTCAACGG+TGG 0.681243 tig0022040:+92269 None:intergenic
GATCCGGATAGTGTGCATGG+AGG 0.746880 tig0022040:-92653 Msa1396590:CDS
TCATAACTTCCTCAGCTACG+AGG 0.747861 tig0022040:-94604 Msa1396590:CDS
TCAAGATCACTACTCCCCAG+AGG 0.760039 tig0022040:-94717 Msa1396590:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGTTTAAATATTAAATAG+CGG - tig0022040:94666-94685 Msa1396590:CDS 10.0%
!! CTCTGATTAAAAAAAAAAAA+AGG + tig0022040:93299-93318 None:intergenic 15.0%
!!! TGTTTTATTACGAATATATA+CGG + tig0022040:93386-93405 None:intergenic 15.0%
!!! TTTTAACAACATAACAAAAA+GGG + tig0022040:93795-93814 None:intergenic 15.0%
!!! TTTTTAACAACATAACAAAA+AGG + tig0022040:93796-93815 None:intergenic 15.0%
!!! TGTTATTTCTATTTAAAATG+AGG - tig0022040:94964-94983 Msa1396590:five_prime_UTR 15.0%
!! TTATGAAGTAAAATAATTCG+TGG + tig0022040:92512-92531 None:intergenic 20.0%
!! TTGTTATGTTGTTAAAAATC+CGG - tig0022040:93798-93817 Msa1396590:intron 20.0%
!!! AGATTGATTTAAATTGCATT+TGG + tig0022040:94051-94070 None:intergenic 20.0%
!!! ATTCTAGAAAACTTGTTATA+TGG - tig0022040:94398-94417 Msa1396590:CDS 20.0%
!! TGTATGATATTAATACACAA+TGG - tig0022040:94718-94737 Msa1396590:CDS 20.0%
! AGAGAAGACATTTATTAACA+TGG - tig0022040:92075-92094 Msa1396590:three_prime_UTR 25.0%
!! TTTTCAATTGCATGTATCAT+AGG + tig0022040:92689-92708 None:intergenic 25.0%
! TCTCAAATTCCAATTGAAAA+TGG - tig0022040:92725-92744 Msa1396590:intron 25.0%
! ATCAGAAAAATAGTCATGAT+GGG + tig0022040:92798-92817 None:intergenic 25.0%
! TATCAGAAAAATAGTCATGA+TGG + tig0022040:92799-92818 None:intergenic 25.0%
!! TCAAATGATAGTTTTCGAAA+AGG + tig0022040:93041-93060 None:intergenic 25.0%
! TATCTTCTGCTATAGATTAA+GGG + tig0022040:93067-93086 None:intergenic 25.0%
! ATATCTTCTGCTATAGATTA+AGG + tig0022040:93068-93087 None:intergenic 25.0%
! TTGAACTCATTATGAATGAA+AGG + tig0022040:93155-93174 None:intergenic 25.0%
! CATTCATAATGAGTTCAATT+TGG - tig0022040:93157-93176 Msa1396590:intron 25.0%
! AGTTCAATTTGGATTATTCT+GGG - tig0022040:93168-93187 Msa1396590:intron 25.0%
!! TTTTCAAAGTAGATAACAAG+TGG - tig0022040:93191-93210 Msa1396590:intron 25.0%
! AAAAAAAAAAGGAAGACACA+AGG + tig0022040:93288-93307 None:intergenic 25.0%
! ATTGCGAAGAATAAAAATTC+CGG + tig0022040:93741-93760 None:intergenic 25.0%
!! TTGTTATTAATGAAGTGGTT+TGG + tig0022040:93962-93981 None:intergenic 25.0%
!! TTTGGTTGTTATTAATGAAG+TGG + tig0022040:93967-93986 None:intergenic 25.0%
! ATGCAATTTAAATCAATCTC+CGG - tig0022040:94052-94071 Msa1396590:intron 25.0%
!! TTTGAAGTGAAAATATGAAG+GGG + tig0022040:94620-94639 None:intergenic 25.0%
!!! CTTTGAAGTGAAAATATGAA+GGG + tig0022040:94621-94640 None:intergenic 25.0%
!!! ACTTTGAAGTGAAAATATGA+AGG + tig0022040:94622-94641 None:intergenic 25.0%
! AGTTTCGATCAATAAAATAC+AGG - tig0022040:94691-94710 Msa1396590:CDS 25.0%
! TGATATTAATACACAATGGA+TGG - tig0022040:94722-94741 Msa1396590:CDS 25.0%
! ATGGTTTGATCTATATCATT+GGG - tig0022040:94741-94760 Msa1396590:CDS 25.0%
CATTTATTAACATGGATCCT+TGG - tig0022040:92083-92102 Msa1396590:three_prime_UTR 30.0%
TCCTTGGAAAAATGAATGAA+AGG - tig0022040:92099-92118 Msa1396590:three_prime_UTR 30.0%
!! TCCTTTCATTCATTTTTCCA+AGG + tig0022040:92103-92122 None:intergenic 30.0%
! ATGATCGAATCGATTCAATT+TGG - tig0022040:92311-92330 Msa1396590:three_prime_UTR 30.0%
! TGATCGAATCGATTCAATTT+GGG - tig0022040:92312-92331 Msa1396590:three_prime_UTR 30.0%
! CTTGAAGTTGTTGTTGTAAA+TGG + tig0022040:92398-92417 None:intergenic 30.0%
! ATAAGCTCTCCATTTTCAAT+TGG + tig0022040:92737-92756 None:intergenic 30.0%
TCAGAAAAATAGTCATGATG+GGG + tig0022040:92797-92816 None:intergenic 30.0%
GCTACAATGCTTATGTATTT+AGG - tig0022040:92968-92987 Msa1396590:intron 30.0%
TATTTAGGTGACAATGTTGA+GGG - tig0022040:92983-93002 Msa1396590:intron 30.0%
AATGAAAGGAATGTGGAATA+AGG + tig0022040:93141-93160 None:intergenic 30.0%
CATTATGAATGAAAGGAATG+TGG + tig0022040:93148-93167 None:intergenic 30.0%
GAGTTCAATTTGGATTATTC+TGG - tig0022040:93167-93186 Msa1396590:intron 30.0%
! ATGTATTTCGTTGTTTCATG+CGG + tig0022040:93343-93362 None:intergenic 30.0%
TGTTGTTGAGTTCTTGATTA+GGG + tig0022040:93432-93451 None:intergenic 30.0%
! TTGTTGTTGAGTTCTTGATT+AGG + tig0022040:93433-93452 None:intergenic 30.0%
! GTATGTTTTATGAATGGTAG+TGG + tig0022040:93715-93734 None:intergenic 30.0%
CATTCATAAAACATACAAGC+CGG - tig0022040:93719-93738 Msa1396590:intron 30.0%
AACAACATAACAAAAAGGGT+GGG + tig0022040:93791-93810 None:intergenic 30.0%
TAACAACATAACAAAAAGGG+TGG + tig0022040:93792-93811 None:intergenic 30.0%
CTAATAAGAAAATCCGGAAA+GGG + tig0022040:93877-93896 None:intergenic 30.0%
ACTAATAAGAAAATCCGGAA+AGG + tig0022040:93878-93897 None:intergenic 30.0%
GGGTAACTAATAAGAAAATC+CGG + tig0022040:93883-93902 None:intergenic 30.0%
TTCCAACTGATAAACAAGTT+TGG - tig0022040:93917-93936 Msa1396590:intron 30.0%
TGCAATTTAAATCAATCTCC+GGG - tig0022040:94053-94072 Msa1396590:intron 30.0%
! ACACTTGCATTTTACACATT+GGG + tig0022040:94139-94158 None:intergenic 30.0%
! AACACTTGCATTTTACACAT+TGG + tig0022040:94140-94159 None:intergenic 30.0%
ATTATCAAACACTCTACTCA+AGG - tig0022040:94186-94205 Msa1396590:CDS 30.0%
TTATGTGTGAAACCAGTTAA+AGG - tig0022040:94284-94303 Msa1396590:intron 30.0%
TAGTATAACTTTGCCTTTGA+AGG - tig0022040:94334-94353 Msa1396590:CDS 30.0%
GATAGTAATTCTTATCTGCT+TGG - tig0022040:94427-94446 Msa1396590:CDS 30.0%
TTATCTGCTTGGTATTATCA+GGG - tig0022040:94438-94457 Msa1396590:CDS 30.0%
AGGGTAAAAAAAAATCACCA+AGG - tig0022040:94588-94607 Msa1396590:CDS 30.0%
GGGTAAAAAAAAATCACCAA+GGG - tig0022040:94589-94608 Msa1396590:CDS 30.0%
GGTAAAAAAAAATCACCAAG+GGG - tig0022040:94590-94609 Msa1396590:CDS 30.0%
GATGGTTTGATCTATATCAT+TGG - tig0022040:94740-94759 Msa1396590:CDS 30.0%
TAAAGATATATCCTTGCGAT+TGG - tig0022040:94773-94792 Msa1396590:CDS 30.0%
!! AGTTGGTCTTAGTACTTAAT+TGG - tig0022040:94832-94851 Msa1396590:intron 30.0%
TTGGATAAGCAGCTAAATTA+AGG - tig0022040:94903-94922 Msa1396590:CDS 30.0%
AAAGAAAGAGATTGACTGAC+TGG - tig0022040:92151-92170 Msa1396590:three_prime_UTR 35.0%
GTCACAATCGGAATGATTAT+TGG - tig0022040:92242-92261 Msa1396590:three_prime_UTR 35.0%
GAAGAAGAAGAAGAAGAAGA+AGG + tig0022040:92286-92305 None:intergenic 35.0%
! CAATTTGGGATTACAATTGC+AGG - tig0022040:92326-92345 Msa1396590:three_prime_UTR 35.0%
TCGTCGTTATTATAACCTCT+GGG + tig0022040:92431-92450 None:intergenic 35.0%
! CTTCAGGTTTAACATATCCA+AGG + tig0022040:92475-92494 None:intergenic 35.0%
TTAAACCTGAAGTCCTTAGT+TGG - tig0022040:92483-92502 Msa1396590:three_prime_UTR 35.0%
ACGAGGAATGTGATTATCTT+AGG - tig0022040:92543-92562 Msa1396590:three_prime_UTR 35.0%
CGAGGAATGTGATTATCTTA+GGG - tig0022040:92544-92563 Msa1396590:three_prime_UTR 35.0%
GAGGAATGTGATTATCTTAG+GGG - tig0022040:92545-92564 Msa1396590:three_prime_UTR 35.0%
!!! CAGTTGAAATTTTGAGACGA+GGG + tig0022040:92580-92599 None:intergenic 35.0%
!!! ACAGTTGAAATTTTGAGACG+AGG + tig0022040:92581-92600 None:intergenic 35.0%
! ACTGATTCTTCTCATACCTA+AGG + tig0022040:92766-92785 None:intergenic 35.0%
AACTGTTAAGCAAGCAGAAA+GGG + tig0022040:92920-92939 None:intergenic 35.0%
! TAACAGTTCAATTTGAAGCG+TGG - tig0022040:92932-92951 Msa1396590:intron 35.0%
GTATTTAGGTGACAATGTTG+AGG - tig0022040:92982-93001 Msa1396590:intron 35.0%
CACAAAAGATACATGCATCT+AGG + tig0022040:93099-93118 None:intergenic 35.0%
! GTATCTTTTGTGTTTTGTCC+CGG - tig0022040:93107-93126 Msa1396590:intron 35.0%
AAGGAATGTGGAATAAGGTT+TGG + tig0022040:93136-93155 None:intergenic 35.0%
! TGTATTTCGTTGTTTCATGC+GGG + tig0022040:93342-93361 None:intergenic 35.0%
TTGTTGAGTTCTTGATTAGG+GGG + tig0022040:93430-93449 None:intergenic 35.0%
GTTGTTGAGTTCTTGATTAG+GGG + tig0022040:93431-93450 None:intergenic 35.0%
! GTGTTGTAGTATCTGTTTTG+AGG + tig0022040:93472-93491 None:intergenic 35.0%
AGAACAACCTCAAAATGACA+AGG - tig0022040:93590-93609 Msa1396590:intron 35.0%
!!! GAGTTTTCCTTGTCATTTTG+AGG + tig0022040:93600-93619 None:intergenic 35.0%
!!! GGTAACGAGGTATTTTTTCT+GGG + tig0022040:93670-93689 None:intergenic 35.0%
!!! AGGTAACGAGGTATTTTTTC+TGG + tig0022040:93671-93690 None:intergenic 35.0%
! CGGCTTGTATGTTTTATGAA+TGG + tig0022040:93721-93740 None:intergenic 35.0%
ATGCGGCTATTTGGATTATT+TGG + tig0022040:93838-93857 None:intergenic 35.0%
! TGTTTATCAGTTGGAAGTGT+TGG + tig0022040:93913-93932 None:intergenic 35.0%
! CACCAAACTTGTTTATCAGT+TGG + tig0022040:93922-93941 None:intergenic 35.0%
CAACTGATAAACAAGTTTGG+TGG - tig0022040:93920-93939 Msa1396590:intron 35.0%
!! AATTGCATTTGGTGTACGAT+GGG + tig0022040:94040-94059 None:intergenic 35.0%
!! AAATTGCATTTGGTGTACGA+TGG + tig0022040:94041-94060 None:intergenic 35.0%
GCAATTTAAATCAATCTCCG+GGG - tig0022040:94054-94073 Msa1396590:intron 35.0%
CAATTTAAATCAATCTCCGG+GGG - tig0022040:94055-94074 Msa1396590:intron 35.0%
AGACGAATGATTTCCTTCAA+AGG + tig0022040:94350-94369 None:intergenic 35.0%
TTTGAAGGAAATCATTCGTC+TGG - tig0022040:94349-94368 Msa1396590:CDS 35.0%
CTTATCTGCTTGGTATTATC+AGG - tig0022040:94437-94456 Msa1396590:CDS 35.0%
!! CGTATGATTTAGCTAGCTTT+CGG - tig0022040:94560-94579 Msa1396590:CDS 35.0%
TACTAAGACCAACTATGACA+AGG + tig0022040:94826-94845 None:intergenic 35.0%
AAATGGAGCTGAAATCTCAA+CGG + tig0022040:94867-94886 None:intergenic 35.0%
AACAAAGCCTTGATCAGAAA+TGG + tig0022040:94884-94903 None:intergenic 35.0%
!! TTTCTGATCAAGGCTTTGTT+TGG - tig0022040:94884-94903 Msa1396590:intron 35.0%
CTTATTTCCACAACTTCTCT+AGG + tig0022040:95020-95039 None:intergenic 35.0%
CTTCTCACTTTCGTCACAAT+CGG - tig0022040:92230-92249 Msa1396590:three_prime_UTR 40.0%
CGTCGTTATTATAACCTCTG+GGG + tig0022040:92430-92449 None:intergenic 40.0%
GTCGTCGTTATTATAACCTC+TGG + tig0022040:92432-92451 None:intergenic 40.0%
TAATTCGTGGAGACCAACTA+AGG + tig0022040:92499-92518 None:intergenic 40.0%
AGTGATGTCAGAACAAGTTC+TGG - tig0022040:92632-92651 Msa1396590:CDS 40.0%
AAAGGGTTGCATTGCAATTG+AGG + tig0022040:92903-92922 None:intergenic 40.0%
GAACTGTTAAGCAAGCAGAA+AGG + tig0022040:92921-92940 None:intergenic 40.0%
! TAAGCATTGTAGCTATTCGC+TGG + tig0022040:92961-92980 None:intergenic 40.0%
AAAAGGAGAAGCTTACCGAT+GGG + tig0022040:93024-93043 None:intergenic 40.0%
TGGAATAAGGTTTGGATTCC+GGG + tig0022040:93128-93147 None:intergenic 40.0%
GTGGAATAAGGTTTGGATTC+CGG + tig0022040:93129-93148 None:intergenic 40.0%
ATACATACGCGACGAAAAAG+AGG - tig0022040:93357-93376 Msa1396590:intron 40.0%
!! TGTTTCTGTCAGGTTTTGAC+AGG + tig0022040:93515-93534 None:intergenic 40.0%
!! GGTGTTTTTGTGTTTCTGTC+AGG + tig0022040:93525-93544 None:intergenic 40.0%
!!! AGGTATTTTTTCTGGGATGC+TGG + tig0022040:93663-93682 None:intergenic 40.0%
!!! GATTGTCTTTTGAGGTAACG+AGG + tig0022040:93683-93702 None:intergenic 40.0%
TACCTCAAAAGACAATCGCT+CGG - tig0022040:93686-93705 Msa1396590:intron 40.0%
!! AGATTTGGTGGAGGAGATTA+AGG + tig0022040:93768-93787 None:intergenic 40.0%
AACAAAAAGGGTGGGAGATT+TGG + tig0022040:93783-93802 None:intergenic 40.0%
TTTGGAAAGCGAGAAATGAC+CGG + tig0022040:93820-93839 None:intergenic 40.0%
ATAAGAAAATCCGGAAAGGG+AGG + tig0022040:93874-93893 None:intergenic 40.0%
!! ATTGCATTTGGTGTACGATG+GGG + tig0022040:94039-94058 None:intergenic 40.0%
CAAGAAGGAATCTTGCGATT+AGG + tig0022040:94090-94109 None:intergenic 40.0%
CTAATCGCAAGATTCCTTCT+TGG - tig0022040:94088-94107 Msa1396590:intron 40.0%
GGTTATAGGCAAATCCAAGA+AGG + tig0022040:94105-94124 None:intergenic 40.0%
GATAGTATGCAATGCGTTTC+AGG - tig0022040:94217-94236 Msa1396590:intron 40.0%
! TGTGGTGGGAAATTAACCAA+AGG - tig0022040:94260-94279 Msa1396590:intron 40.0%
! GTGGTGGGAAATTAACCAAA+GGG - tig0022040:94261-94280 Msa1396590:intron 40.0%
TGGTTTCACACATAACCCTT+TGG + tig0022040:94279-94298 None:intergenic 40.0%
AGCACAGCATTTCCTTTAAC+TGG + tig0022040:94299-94318 None:intergenic 40.0%
! AAGGAAATGCTGTGCTTTTC+TGG - tig0022040:94303-94322 Msa1396590:intron 40.0%
!! ATGATTTAGCTAGCTTTCGG+TGG - tig0022040:94563-94582 Msa1396590:CDS 40.0%
!! TGATTTAGCTAGCTTTCGGT+GGG - tig0022040:94564-94583 Msa1396590:CDS 40.0%
AAATACTGAGACCAATCGCA+AGG + tig0022040:94787-94806 None:intergenic 40.0%
TCATGTGACCTTGTCATAGT+TGG - tig0022040:94815-94834 Msa1396590:intron 40.0%
TTCAGCTCCATTTCTGATCA+AGG - tig0022040:94874-94893 Msa1396590:intron 40.0%
!! AACTTCTCTAGGATAGCTTG+CGG + tig0022040:95009-95028 None:intergenic 40.0%
AAGCTATCCTAGAGAAGTTG+TGG - tig0022040:95010-95029 Msa1396590:five_prime_UTR 40.0%
AATCGAATCGATGAGTCCTC+CGG - tig0022040:92187-92206 Msa1396590:three_prime_UTR 45.0%
GAAGCACAAATCTTACGCCT+TGG - tig0022040:92455-92474 Msa1396590:three_prime_UTR 45.0%
TCATAACTTCCTCAGCTACG+AGG - tig0022040:92526-92545 Msa1396590:three_prime_UTR 45.0%
AATCACATTCCTCGTAGCTG+AGG + tig0022040:92538-92557 None:intergenic 45.0%
GTCGATGCAAACACAGGAAA+GGG - tig0022040:92602-92621 Msa1396590:CDS 45.0%
GAGAGCTTATGCAAGTCCTT+AGG - tig0022040:92747-92766 Msa1396590:intron 45.0%
! CAGTTCAATTTGAAGCGTGG+TGG - tig0022040:92935-92954 Msa1396590:intron 45.0%
TTAGGTGACAATGTTGAGGG+AGG - tig0022040:92986-93005 Msa1396590:intron 45.0%
TAGGTGACAATGTTGAGGGA+GGG - tig0022040:92987-93006 Msa1396590:intron 45.0%
GAAAAGGAGAAGCTTACCGA+TGG + tig0022040:93025-93044 None:intergenic 45.0%
!!! GGCTGTTTTGAGGTTCTATG+GGG + tig0022040:93409-93428 None:intergenic 45.0%
!!! GGGCTGTTTTGAGGTTCTAT+GGG + tig0022040:93410-93429 None:intergenic 45.0%
!! TTGATTAGGGGGCTGTTTTG+AGG + tig0022040:93419-93438 None:intergenic 45.0%
!!! ATTTTTTCTGGGATGCTGGC+AGG + tig0022040:93659-93678 None:intergenic 45.0%
ACCTCAAAAGACAATCGCTC+GGG - tig0022040:93687-93706 Msa1396590:intron 45.0%
AAAAAGGGTGGGAGATTTGG+TGG + tig0022040:93780-93799 None:intergenic 45.0%
!! TCAGTTGGAAGTGTTGGAGT+GGG + tig0022040:93907-93926 None:intergenic 45.0%
!! ATCAGTTGGAAGTGTTGGAG+TGG + tig0022040:93908-93927 None:intergenic 45.0%
CTTATTCCTGCATTGCGAGT+TGG + tig0022040:93999-94018 None:intergenic 45.0%
!! TTGCATTTGGTGTACGATGG+GGG + tig0022040:94038-94057 None:intergenic 45.0%
GATTAGGAATGTCTTACCCC+CGG + tig0022040:94074-94093 None:intergenic 45.0%
GGGCATGTTTCTCAGGTTAT+AGG + tig0022040:94119-94138 None:intergenic 45.0%
ACACATTGGGCATGTTTCTC+AGG + tig0022040:94126-94145 None:intergenic 45.0%
GGTTCAGATGAATGTAGCTG+TGG - tig0022040:94242-94261 Msa1396590:intron 45.0%
TCAGATGAATGTAGCTGTGG+TGG - tig0022040:94245-94264 Msa1396590:intron 45.0%
CAGATGAATGTAGCTGTGGT+GGG - tig0022040:94246-94265 Msa1396590:intron 45.0%
!!! TGCTGTGCTTTTCTGGAGTA+TGG - tig0022040:94310-94329 Msa1396590:intron 45.0%
TGCTTGGTATTATCAGGGAC+TGG - tig0022040:94443-94462 Msa1396590:CDS 45.0%
TGGTATTATCAGGGACTGGA+TGG - tig0022040:94447-94466 Msa1396590:CDS 45.0%
ATATGAAGGGGAAAACCCCT+TGG + tig0022040:94608-94627 None:intergenic 45.0%
AACACAATCTTCATCGCCGG+AGG + tig0022040:92206-92225 None:intergenic 50.0%
CCGAACACAATCTTCATCGC+CGG + tig0022040:92209-92228 None:intergenic 50.0%
CCGGCGATGAAGATTGTGTT+CGG - tig0022040:92206-92225 Msa1396590:three_prime_UTR 50.0%
TGCAGGTGCTCTCTCTCAAT+TGG - tig0022040:92343-92362 Msa1396590:three_prime_UTR 50.0%
TCAAGATCACTACTCCCCAG+AGG - tig0022040:92413-92432 Msa1396590:three_prime_UTR 50.0%
GGAGACCAACTAAGGACTTC+AGG + tig0022040:92491-92510 None:intergenic 50.0%
ACTGTCGTCGATGCAAACAC+AGG - tig0022040:92596-92615 Msa1396590:CDS 50.0%
CGTCGATGCAAACACAGGAA+AGG - tig0022040:92601-92620 Msa1396590:CDS 50.0%
AGGTGACAATGTTGAGGGAG+GGG - tig0022040:92988-93007 Msa1396590:intron 50.0%
AGGGGAAACGCATTTCCCAT+CGG - tig0022040:93006-93025 Msa1396590:intron 50.0%
!!! GGGGCTGTTTTGAGGTTCTA+TGG + tig0022040:93411-93430 None:intergenic 50.0%
! GCTTTTGAGCTGTTGCGCTT+GGG + tig0022040:93554-93573 None:intergenic 50.0%
! TGCTTTTGAGCTGTTGCGCT+TGG + tig0022040:93555-93574 None:intergenic 50.0%
! ACCCGAGCGATTGTCTTTTG+AGG + tig0022040:93691-93710 None:intergenic 50.0%
TAGAGTGGCATGCGGCTATT+TGG + tig0022040:93847-93866 None:intergenic 50.0%
ACTCTATCCACCTCCCTTTC+CGG - tig0022040:93861-93880 Msa1396590:intron 50.0%
AGAAAATCCGGAAAGGGAGG+TGG + tig0022040:93871-93890 None:intergenic 50.0%
!! AGTTGGAAGTGTTGGAGTGG+GGG + tig0022040:93905-93924 None:intergenic 50.0%
!! CAGTTGGAAGTGTTGGAGTG+GGG + tig0022040:93906-93925 None:intergenic 50.0%
GTATGCAATGCGTTTCAGGC+TGG - tig0022040:94221-94240 Msa1396590:intron 50.0%
ACTGGATGGACAGTCAGATC+CGG - tig0022040:94461-94480 Msa1396590:CDS 50.0%
TCAGATCCGGATAGTGTGCA+TGG - tig0022040:94474-94493 Msa1396590:CDS 50.0%
CATCCTCCATGCACACTATC+CGG + tig0022040:94483-94502 None:intergenic 50.0%
AGAAGTGGTCGGCTACAAAG+TGG - tig0022040:94520-94539 Msa1396590:CDS 50.0%
!! TTAGCTAGCTTTCGGTGGGT+AGG - tig0022040:94568-94587 Msa1396590:CDS 50.0%
!! TAGCTAGCTTTCGGTGGGTA+GGG - tig0022040:94569-94588 Msa1396590:CDS 50.0%
TGGAGCTGAAATCTCAACGG+TGG + tig0022040:94864-94883 None:intergenic 50.0%
!! AAGGGTGGGAGATTTGGTGG+AGG + tig0022040:93777-93796 None:intergenic 55.0%
GAGGTGGATAGAGTGGCATG+CGG + tig0022040:93855-93874 None:intergenic 55.0%
GGAAAGGGAGGTGGATAGAG+TGG + tig0022040:93862-93881 None:intergenic 55.0%
!! TTGGAAGTGTTGGAGTGGGG+GGG + tig0022040:93903-93922 None:intergenic 55.0%
!! GTTGGAAGTGTTGGAGTGGG+GGG + tig0022040:93904-93923 None:intergenic 55.0%
! GCGAGTTGGCGTGGAATGTT+TGG + tig0022040:93985-94004 None:intergenic 55.0%
GATCCGGATAGTGTGCATGG+AGG - tig0022040:94477-94496 Msa1396590:CDS 55.0%
TAGTGTGCATGGAGGATGCC+CGG - tig0022040:94485-94504 Msa1396590:CDS 55.0%
ACTTCTCCCCAGCAAGTACC+GGG + tig0022040:94506-94525 None:intergenic 55.0%
CACTTCTCCCCAGCAAGTAC+CGG + tig0022040:94507-94526 None:intergenic 55.0%
! ACTTGCTGGGGAGAAGTGGT+CGG - tig0022040:94509-94528 Msa1396590:CDS 55.0%
GGTCGGCTACAAAGTGGATG+AGG - tig0022040:94526-94545 Msa1396590:CDS 55.0%
! GCTGTTGCGCTTGGGTTGCT+GGG + tig0022040:93546-93565 None:intergenic 60.0%
! AGCTGTTGCGCTTGGGTTGC+TGG + tig0022040:93547-93566 None:intergenic 60.0%
TCCACGCCAACTCGCAATGC+AGG - tig0022040:93990-94009 Msa1396590:intron 60.0%
! TCCTGCATTGCGAGTTGGCG+TGG + tig0022040:93994-94013 None:intergenic 60.0%
GAGGATGCCCGGTACTTGCT+GGG - tig0022040:94496-94515 Msa1396590:CDS 60.0%
! AGGATGCCCGGTACTTGCTG+GGG - tig0022040:94497-94516 Msa1396590:CDS 60.0%
! CGGTACTTGCTGGGGAGAAG+TGG - tig0022040:94505-94524 Msa1396590:CDS 60.0%
! TGCTGGCAGGTGCTGTGGGT+TGG + tig0022040:93646-93665 None:intergenic 65.0%
! GGGATGCTGGCAGGTGCTGT+GGG + tig0022040:93650-93669 None:intergenic 65.0%
! TGGGATGCTGGCAGGTGCTG+TGG + tig0022040:93651-93670 None:intergenic 65.0%
GGAGGATGCCCGGTACTTGC+TGG - tig0022040:94495-94514 Msa1396590:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0022040 gene 92054 95098 92054 ID=Msa1396590;Name=Msa1396590
tig0022040 mRNA 92054 95098 92054 ID=Msa1396590-mRNA-1;Parent=Msa1396590;Name=Msa1396590-mRNA-1;_AED=0.07;_eAED=0.07;_QI=143|1|1|1|1|1|5|532|276
tig0022040 exon 92054 92693 92054 ID=Msa1396590-mRNA-1:exon:15570;Parent=Msa1396590-mRNA-1
tig0022040 exon 92821 92913 92821 ID=Msa1396590-mRNA-1:exon:15569;Parent=Msa1396590-mRNA-1
tig0022040 exon 94125 94214 94125 ID=Msa1396590-mRNA-1:exon:15568;Parent=Msa1396590-mRNA-1
tig0022040 exon 94332 94804 94332 ID=Msa1396590-mRNA-1:exon:15567;Parent=Msa1396590-mRNA-1
tig0022040 exon 94889 95098 94889 ID=Msa1396590-mRNA-1:exon:15566;Parent=Msa1396590-mRNA-1
tig0022040 five_prime_UTR 94956 95098 94956 ID=Msa1396590-mRNA-1:five_prime_utr;Parent=Msa1396590-mRNA-1
tig0022040 CDS 94889 94955 94889 ID=Msa1396590-mRNA-1:cds;Parent=Msa1396590-mRNA-1
tig0022040 CDS 94332 94804 94332 ID=Msa1396590-mRNA-1:cds;Parent=Msa1396590-mRNA-1
tig0022040 CDS 94125 94214 94125 ID=Msa1396590-mRNA-1:cds;Parent=Msa1396590-mRNA-1
tig0022040 CDS 92821 92913 92821 ID=Msa1396590-mRNA-1:cds;Parent=Msa1396590-mRNA-1
tig0022040 CDS 92586 92693 92586 ID=Msa1396590-mRNA-1:cds;Parent=Msa1396590-mRNA-1
tig0022040 three_prime_UTR 92054 92585 92054 ID=Msa1396590-mRNA-1:three_prime_utr;Parent=Msa1396590-mRNA-1
Gene Sequence

>Msa1396590

ATGAGTCCTCCGGCGATGAAGATTGTGTTCGGACTTCTCACTTTCGTCACAATCGGAATGATTATTGGTGCTCTCTCTCAATTGGCATTCATACGCAGATTAGAACTAGAAGAACCATTTACAACAACAACTTCAAGATCACTACTCCCCAGAGGTTATAATAACGACGACAAAGAAGCACAAATCTTACGCCTTGGATATGTTAAACCTGAAGTCCTTAGTTGGTCTCCACGAATTATTTTACTTCATAACTTCCTCAGCTACGAGGAATGTGATTATCTTAGGGGAGTTGCTCTCCCTCGTCTCAAAATTTCAACTGTCGTCGATGCAAACACAGGAAAGGGAATCAAGAGTGATGTCAGAACAAGTTCTGGTATGTTTCTGAATCATGAAGAGAGAAAATATCCTATGATACATGCAATTGAAAAAAGAATTTCTGTCTACTCTCAAATTCCAATTGAAAATGGAGAGCTTATGCAAGTCCTTAGGTATGAGAAGAATCAGTATTACAGACCCCATCATGACTATTTTTCTGATACTTTCAATTTGAAGCGTGGTGGCCAGCGAATAGCTACAATGCTTATGTATTTAGGTGACAATGTTGAGGGAGGGGAAACGCATTTCCCATCGGCTGGTTCAGATGAATGTAGCTGTGGTGGGAAATTAACCAAAGGGTTATGTGTGAAACCAGTTAAAGGAAATGCTGTGCTTTTCTGGAGTATGGGACTGGATGGACAGTCAGATCCGGATAGTGTGCATGGAGGATGCCCGGTACTTGCTGGGGAGAAGTGGTCGGCTACAAAGTGGATGAGGCAATCAGTTCACGTATGA
Protein sequence

>Msa1396590

MSPPAMKIVFGLLTFVTIGMIIGALSQLAFIRRLELEEPFTTTTSRSLLPRGYNNDDKEAQILRLGYVKPEVLSWSPRIILLHNFLSYEECDYLRGVALPRLKISTVVDANTGKGIKSDVRTSSGMFLNHEERKYPMIHAIEKRISVYSQIPIENGELMQVLRYEKNQYYRPHHDYFSDTFNLKRGGQRIATMLMYLGDNVEGGETHFPSAGSDECSCGGKLTKGLCVKPVKGNAVLFWSMGLDGQSDPDSVHGGCPVLAGEKWSATKWMRQSVHV*