AlfalfaGEDB | Msa1403410

M. sativa cultivar zhongmu-4 / Msa1403410

KEGG: -
GO: GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009787 GO:0009788 GO:0009889 GO:0009966 GO:0009968 GO:0010468 GO:0010556 GO:0010646 GO:0010648 GO:0019219 GO:0019222 GO:0023051 GO:0023057 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044212 GO:0044424 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901419 GO:1901420 GO:1903506 GO:1905957 GO:1905958 GO:2000112 GO:2001141
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1403410	XP_013454510.1	96.296	216	7	1	1	216	10	224	1.06e-149	425

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1403410	sp\|Q42485\|ZFP1_ARATH	38.428	229	92	7	15	216	22	228	3.72e-32	119

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1403410	G7JD88	96.296	216	7	1	1	216	10	224	5.04e-150	425

TFs/TRs:

Gene ID	Type	Classification
Msa1403410	TF	C2H2

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0625690	Msa1403410	0.890560	8.821018e-74	-8.615850e-47
Msa0529860	Msa1403410	0.958079	5.707176e-116	-8.615850e-47
Msa0585520	Msa1403410	0.971806	9.528333e-134	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1403410	MtrunA17_Chr4g0001081	96.296	216	7	1	1	216	10	224	9.71e-154	425
Msa1403410	MtrunA17_Chr2g0333741	51.899	237	80	11	3	216	22	247	9.31e-60	187
Msa1403410	MtrunA17_Chr1g0149811	38.559	236	95	10	12	216	46	262	2.53e-34	123
Msa1403410	MtrunA17_Chr3g0137111	36.893	206	95	7	15	189	25	226	1.27e-26	102
Msa1403410	MtrunA17_Chr8g0376021	43.966	116	53	2	13	122	23	132	7.36e-24	94.7
Msa1403410	MtrunA17_Chr5g0434121	50.413	121	46	4	7	118	38	153	1.96e-21	88.6
Msa1403410	MtrunA17_Chr1g0207691	44.231	104	55	1	23	123	29	132	1.78e-19	82.4
Msa1403410	MtrunA17_Chr1g0161901	56.944	72	24	2	58	122	97	168	3.91e-19	82.8
Msa1403410	MtrunA17_Chr3g0110091	60.274	73	21	3	53	118	80	151	1.77e-18	80.9
Msa1403410	MtrunA17_Chr4g0053691	45.455	110	41	4	58	165	67	159	4.17e-18	78.6
Msa1403410	MtrunA17_Chr4g0072571	65.385	52	16	1	55	106	42	91	3.58e-16	73.2
Msa1403410	MtrunA17_Chr5g0396031	40.909	110	41	3	37	128	25	128	7.07e-16	75.5
Msa1403410	MtrunA17_Chr3g0081131	42.157	102	49	3	37	128	76	177	6.16e-14	69.3
Msa1403410	MtrunA17_Chr3g0081141	38.525	122	53	3	38	140	77	195	2.18e-13	67.8
Msa1403410	MtrunA17_Chr7g0254221	76.471	34	8	0	58	91	33	66	2.88e-12	62.4
Msa1403410	MtrunA17_Chr7g0251581	75.758	33	8	0	58	90	39	71	2.91e-12	63.2
Msa1403410	MtrunA17_Chr3g0081121	40.909	88	37	2	56	128	96	183	4.12e-12	63.9
Msa1403410	MtrunA17_Chr7g0222661	28.788	198	97	9	10	176	39	223	2.52e-11	61.6
Msa1403410	MtrunA17_Chr7g0239711	48.214	56	29	0	58	113	57	112	7.51e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1403410	AT1G80730.1	38.428	229	92	7	15	216	22	228	3.78e-33	119
Msa1403410	AT5G25160.1	42.593	216	87	10	27	216	31	235	1.56e-31	115
Msa1403410	AT5G10970.1	39.726	219	113	8	13	216	58	272	3.15e-31	115
Msa1403410	AT1G24625.1	62.121	66	24	1	54	118	53	118	1.90e-20	85.9
Msa1403410	AT1G66140.1	48.485	99	23	3	58	128	83	181	1.92e-17	79.0
Msa1403410	AT5G57520.1	70.833	48	14	0	57	104	49	96	2.24e-16	73.6
Msa1403410	AT5G14010.1	79.412	34	7	0	58	91	36	69	2.12e-13	65.9
Msa1403410	AT5G01860.1	62.222	45	17	0	47	91	54	98	7.31e-13	65.5

Find 33 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTTCTTCACTACCTTCTTA+TGG	0.229495	tig0022956:+27417	Msa1403410:CDS
GGGTAGCCATGTTTCCTAAA+AGG	0.278959	tig0022956:+27646	Msa1403410:CDS
TTTCGCGCTTGTGTGCATTC+TGG	0.297005	tig0022956:-27356	None:intergenic
GAGAACTATAAAACTTTCTT+TGG	0.300653	tig0022956:-27317	None:intergenic
TTGTTTCCATGCAAATTTAT+CGG	0.310453	tig0022956:+27230	Msa1403410:CDS
AGGACTTGGAGGTTACTTAT+TGG	0.313639	tig0022956:+27680	Msa1403410:CDS
AATGGAAGCTTTCCAATTGC+TGG	0.323356	tig0022956:-27567	None:intergenic
AAAACGGGTGTTTGATAAGA+AGG	0.401545	tig0022956:-27489	None:intergenic
TTTGATGCACAACCAGCAAT+TGG	0.402242	tig0022956:+27555	Msa1403410:CDS
CTCACATGAAAATTTCCTAA+TGG	0.404461	tig0022956:-27585	None:intergenic
GCAATTGGAAAGCTTCCATT+AGG	0.416438	tig0022956:+27570	Msa1403410:CDS
CCTTTGGTGGTAGCATTTCA+AGG	0.427189	tig0022956:+27625	Msa1403410:CDS
CCTAAAAGGCTTGCAAATGA+AGG	0.436826	tig0022956:+27660	Msa1403410:CDS
GATGAAGTTGAATCATCCTT+TGG	0.437021	tig0022956:+27609	Msa1403410:CDS
AGGCTTGCAAATGAAGGACT+TGG	0.478799	tig0022956:+27666	Msa1403410:CDS
GTTCAAATGGACAAAATGGA+TGG	0.492994	tig0022956:+27520	Msa1403410:CDS
TTGTTACACAAGCCATAAGA+AGG	0.503153	tig0022956:-27429	None:intergenic
CTTGCAAATGAAGGACTTGG+AGG	0.517674	tig0022956:+27669	Msa1403410:CDS
TATAGTTCTCAAGCACTTGG+AGG	0.518487	tig0022956:+27330	Msa1403410:CDS
AATTTGCATGGAAACAATTG+AGG	0.530917	tig0022956:-27224	None:intergenic
GGCTTGTGTAACAAATCACT+TGG	0.540135	tig0022956:+27438	Msa1403410:CDS
ACACAAGCGCGAAAGAACTT+TGG	0.541231	tig0022956:+27365	Msa1403410:CDS
AGCTTTCCGATAAATTTGCA+TGG	0.561003	tig0022956:-27236	None:intergenic
CCTTGAAATGCTACCACCAA+AGG	0.566032	tig0022956:-27625	None:intergenic
TCTGACTTTGAATCACTACA+TGG	0.579513	tig0022956:-27192	None:intergenic
AAATTTATCGGAAAGCTCCT+CGG	0.581574	tig0022956:+27242	Msa1403410:CDS
TTGCATGGAAACAATTGAGG+AGG	0.582251	tig0022956:-27221	None:intergenic
GAAAGAACTTTGGCGAGAAG+AGG	0.587899	tig0022956:+27375	Msa1403410:CDS
TGATCTAAGTCTCTCTAGCA+AGG	0.597107	tig0022956:+27167	Msa1403410:CDS
AAGAGGCTATACAGCTAGCA+TGG	0.598640	tig0022956:+27392	Msa1403410:CDS
CTTTGGTGGTAGCATTTCAA+GGG	0.599828	tig0022956:+27626	Msa1403410:CDS
GAAGTTGAATCATCCTTTGG+TGG	0.613485	tig0022956:+27612	Msa1403410:CDS
ATTCTCATGACTAGATTCCG+AGG	0.661115	tig0022956:-27259	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTTGAACAAAATCCAAAAA+CGG	-	tig0022956:27508-27527	None:intergenic	20.0%
!	TTGTTTCCATGCAAATTTAT+CGG	+	tig0022956:27230-27249	Msa1403410:CDS	25.0%
!	GAGAACTATAAAACTTTCTT+TGG	-	tig0022956:27320-27339	None:intergenic	25.0%
!	TTTGAACAAAATCCAAAAAC+GGG	-	tig0022956:27507-27526	None:intergenic	25.0%
!!!	GTTTTTGGATTTTGTTCAAA+TGG	+	tig0022956:27507-27526	Msa1403410:CDS	25.0%
!!	TTTTGTTCAAATGGACAAAA+TGG	+	tig0022956:27516-27535	Msa1403410:CDS	25.0%
!!	ATTAGGAAATTTTCATGTGA+GGG	+	tig0022956:27587-27606	Msa1403410:CDS	25.0%
	AATTTGCATGGAAACAATTG+AGG	-	tig0022956:27227-27246	None:intergenic	30.0%
	TAGTTGCATGAAAAAACTCT+CGG	-	tig0022956:27294-27313	None:intergenic	30.0%
!	TTTTATAGTTCTCAAGCACT+TGG	+	tig0022956:27327-27346	Msa1403410:CDS	30.0%
	CTCACATGAAAATTTCCTAA+TGG	-	tig0022956:27588-27607	None:intergenic	30.0%
!	CATTAGGAAATTTTCATGTG+AGG	+	tig0022956:27586-27605	Msa1403410:CDS	30.0%
	TCTGACTTTGAATCACTACA+TGG	-	tig0022956:27195-27214	None:intergenic	35.0%
	AGCTTTCCGATAAATTTGCA+TGG	-	tig0022956:27239-27258	None:intergenic	35.0%
	AAATTTATCGGAAAGCTCCT+CGG	+	tig0022956:27242-27261	Msa1403410:CDS	35.0%
	TCTTCTTCACTACCTTCTTA+TGG	+	tig0022956:27417-27436	Msa1403410:CDS	35.0%
	TTGTTACACAAGCCATAAGA+AGG	-	tig0022956:27432-27451	None:intergenic	35.0%
	AAAACGGGTGTTTGATAAGA+AGG	-	tig0022956:27492-27511	None:intergenic	35.0%
!	TCTTATCAAACACCCGTTTT+TGG	+	tig0022956:27492-27511	Msa1403410:CDS	35.0%
	GTTCAAATGGACAAAATGGA+TGG	+	tig0022956:27520-27539	Msa1403410:CDS	35.0%
	GATGAAGTTGAATCATCCTT+TGG	+	tig0022956:27609-27628	Msa1403410:CDS	35.0%
	TGATCTAAGTCTCTCTAGCA+AGG	+	tig0022956:27167-27186	Msa1403410:CDS	40.0%
	TTGCATGGAAACAATTGAGG+AGG	-	tig0022956:27224-27243	None:intergenic	40.0%
	ATTCTCATGACTAGATTCCG+AGG	-	tig0022956:27262-27281	None:intergenic	40.0%
	TATAGTTCTCAAGCACTTGG+AGG	+	tig0022956:27330-27349	Msa1403410:CDS	40.0%
	GGCTTGTGTAACAAATCACT+TGG	+	tig0022956:27438-27457	Msa1403410:CDS	40.0%
	TTTGATGCACAACCAGCAAT+TGG	+	tig0022956:27555-27574	Msa1403410:CDS	40.0%
	AATGGAAGCTTTCCAATTGC+TGG	-	tig0022956:27570-27589	None:intergenic	40.0%
	GCAATTGGAAAGCTTCCATT+AGG	+	tig0022956:27570-27589	Msa1403410:CDS	40.0%
	GAAGTTGAATCATCCTTTGG+TGG	+	tig0022956:27612-27631	Msa1403410:CDS	40.0%
!	CTTTGGTGGTAGCATTTCAA+GGG	+	tig0022956:27626-27645	Msa1403410:CDS	40.0%
!!	TGCAAGCCTTTTAGGAAACA+TGG	-	tig0022956:27655-27674	None:intergenic	40.0%
!	CCTTCATTTGCAAGCCTTTT+AGG	-	tig0022956:27663-27682	None:intergenic	40.0%
	CCTAAAAGGCTTGCAAATGA+AGG	+	tig0022956:27660-27679	Msa1403410:CDS	40.0%
	AGGACTTGGAGGTTACTTAT+TGG	+	tig0022956:27680-27699	Msa1403410:CDS	40.0%
	ACACAAGCGCGAAAGAACTT+TGG	+	tig0022956:27365-27384	Msa1403410:CDS	45.0%
	GAAAGAACTTTGGCGAGAAG+AGG	+	tig0022956:27375-27394	Msa1403410:CDS	45.0%
	AAGAGGCTATACAGCTAGCA+TGG	+	tig0022956:27392-27411	Msa1403410:CDS	45.0%
	CCTTGAAATGCTACCACCAA+AGG	-	tig0022956:27628-27647	None:intergenic	45.0%
!	CCTTTGGTGGTAGCATTTCA+AGG	+	tig0022956:27625-27644	Msa1403410:CDS	45.0%
	GGGTAGCCATGTTTCCTAAA+AGG	+	tig0022956:27646-27665	Msa1403410:CDS	45.0%
	AGGCTTGCAAATGAAGGACT+TGG	+	tig0022956:27666-27685	Msa1403410:CDS	45.0%
	CTTGCAAATGAAGGACTTGG+AGG	+	tig0022956:27669-27688	Msa1403410:CDS	45.0%
	TTTCGCGCTTGTGTGCATTC+TGG	-	tig0022956:27359-27378	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
tig0022956	gene	27123	27773	27123	ID=Msa1403410;Name=Msa1403410
tig0022956	mRNA	27123	27773	27123	ID=Msa1403410-mRNA-1;Parent=Msa1403410;Name=Msa1403410-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|216
tig0022956	exon	27123	27773	27123	ID=Msa1403410-mRNA-1:exon:19257;Parent=Msa1403410-mRNA-1
tig0022956	CDS	27123	27773	27123	ID=Msa1403410-mRNA-1:cds;Parent=Msa1403410-mRNA-1

Gene Sequence

>Msa1403410

ATGGATAGAAAACACAAAAATGATTCATCTTCTCAACTTGTACTTGATCTAAGTCTCTCTAGCAAGGATCCATGTAGTGATTCAAAGTCAGAACTTAACCTCCTCAATTGTTTCCATGCAAATTTATCGGAAAGCTCCTCGGAATCTAGTCATGAGAATGAACTTGATCCGAGAGTTTTTTCATGCAACTACTGCCAAAGAAAGTTTTATAGTTCTCAAGCACTTGGAGGACACCAGAATGCACACAAGCGCGAAAGAACTTTGGCGAGAAGAGGCTATACAGCTAGCATGGCTTCTTCTTCACTACCTTCTTATGGCTTGTGTAACAAATCACTTGGCATTCAAGTGCATTCTATGATTAACAAACCTTCTTATCAAACACCCGTTTTTGGATTTTGTTCAAATGGACAAAATGGATGGAGAAGACAAAACTTTGATGCACAACCAGCAATTGGAAAGCTTCCATTAGGAAATTTTCATGTGAGGGATGAAGTTGAATCATCCTTTGGTGGTAGCATTTCAAGGGTAGCCATGTTTCCTAAAAGGCTTGCAAATGAAGGACTTGGAGGTTACTTATTGGATAGTAACACTTCTCATTTGCAGTCTAAACAAGAGAAGTTGCAAAAACTTGACTTATCTCTTAAGCTCTAA

Protein sequence

>Msa1403410

MDRKHKNDSSSQLVLDLSLSSKDPCSDSKSELNLLNCFHANLSESSSESSHENELDPRVFSCNYCQRKFYSSQALGGHQNAHKRERTLARRGYTASMASSSLPSYGLCNKSLGIQVHSMINKPSYQTPVFGFCSNGQNGWRRQNFDAQPAIGKLPLGNFHVRDEVESSFGGSISRVAMFPKRLANEGLGGYLLDSNTSHLQSKQEKLQKLDLSLKL*