AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1410400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1410400 MtrunA17_Chr4g0071041 97.059 476 11 1 1 476 1 473 0.0 960
Msa1410400 MtrunA17_Chr2g0299641 74.839 465 90 5 20 472 57 506 0.0 710
Msa1410400 MtrunA17_Chr8g0342561 64.169 427 146 3 51 476 84 504 0.0 557
Msa1410400 MtrunA17_Chr2g0330061 56.618 408 148 6 64 465 80 464 1.14e-152 442
Msa1410400 MtrunA17_Chr8g0378761 60.172 349 123 5 9 346 55 398 3.37e-142 422
Msa1410400 MtrunA17_Chr8g0378761 44.706 85 34 3 359 433 513 594 1.52e-12 69.7
Msa1410400 MtrunA17_Chr3g0121261 68.100 279 81 3 85 356 6 283 5.43e-139 401
Msa1410400 MtrunA17_Chr1g0176231 69.343 274 83 1 74 347 67 339 3.34e-135 402
Msa1410400 MtrunA17_Chr1g0176231 47.059 85 36 3 383 467 488 563 7.58e-13 70.9
Msa1410400 MtrunA17_Chr1g0178991 47.964 442 173 8 64 451 46 484 4.04e-125 373
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1410400 AT4G33050.3 69.586 411 112 3 59 469 87 484 0.0 579
Msa1410400 AT4G33050.5 69.586 411 112 3 59 469 159 556 0.0 579
Msa1410400 AT4G33050.2 63.697 449 112 4 59 469 87 522 0.0 561
Msa1410400 AT5G57010.1 60.573 454 143 7 25 470 70 495 0.0 545
Msa1410400 AT2G26190.3 68.889 405 120 5 68 469 126 527 0.0 543
Msa1410400 AT2G26190.1 68.889 405 120 5 68 469 126 527 0.0 543
Msa1410400 AT2G26190.2 79.853 273 54 1 68 340 126 397 2.71e-159 458
Msa1410400 AT3G13600.1 68.750 288 84 3 69 351 96 382 9.26e-143 422
Msa1410400 AT3G13600.1 51.807 83 35 2 386 467 520 598 1.16e-15 80.1
Msa1410400 AT3G13600.2 68.750 288 84 3 69 351 96 382 9.26e-143 422
Msa1410400 AT3G13600.2 51.807 83 35 2 386 467 520 598 1.16e-15 80.1
Msa1410400 AT4G33050.4 77.200 250 56 1 59 308 87 335 3.39e-138 401
Msa1410400 AT3G58480.1 69.630 270 78 2 81 347 98 366 8.11e-135 401
Msa1410400 AT3G58480.1 38.889 90 40 2 371 445 467 556 1.54e-12 70.1
Msa1410400 AT4G33050.1 67.014 288 56 2 59 308 87 373 1.80e-130 383
Msa1410400 AT3G52870.1 48.157 407 168 6 81 445 49 454 2.12e-130 385
Msa1410400 AT4G33050.6 81.287 171 31 1 59 229 87 256 6.01e-97 293

Find 126 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAATATTTATTATATATT+TGG 0.096685 tig0023654:-43700 None:intergenic
CTTTAACTTGCAGTTGAAAT+TGG 0.107738 tig0023654:-46027 None:intergenic
AAAGAATTTATAAGCTTTCT+TGG 0.184873 tig0023654:+45478 Msa1410400:CDS
GCTTGAAGTACTCATCTTAT+TGG 0.205759 tig0023654:-43825 None:intergenic
TTAATTGATCGTACAGTTAT+TGG 0.216708 tig0023654:+43591 Msa1410400:five_prime_UTR
AACAAATTAATGCAATTATT+TGG 0.224184 tig0023654:+43670 Msa1410400:five_prime_UTR
CTAGGTGACATTCTAACTTT+TGG 0.235750 tig0023654:-45970 None:intergenic
TTCACATGTATTATGATATC+TGG 0.237835 tig0023654:+44578 Msa1410400:CDS
CAATTTCAACTGCAAGTTAA+AGG 0.240605 tig0023654:+46028 Msa1410400:CDS
CAGTTATTGGAGGTTAATTA+TGG 0.241480 tig0023654:+43604 Msa1410400:five_prime_UTR
GAATGACCCTCTAAATTCTT+TGG 0.243996 tig0023654:-46184 None:intergenic
CAACGTGACACAGCAGTTTC+AGG 0.247868 tig0023654:-44181 None:intergenic
GCAGTTGAAATTGGGTTTCT+TGG 0.258697 tig0023654:-46018 None:intergenic
TGTGACATCAACTCATAAAA+AGG 0.281124 tig0023654:+45753 Msa1410400:CDS
TTTGTGGGGCATTATATTTA+TGG 0.288333 tig0023654:+43772 Msa1410400:exon
TGAATATTTATTATATATTT+GGG 0.291210 tig0023654:-43699 None:intergenic
CGTTGTAGAACAGTGCTTCT+TGG 0.292853 tig0023654:-44802 None:intergenic
CAACATTCAAGCTTTCTTGC+TGG 0.301504 tig0023654:+45100 Msa1410400:CDS
ATGTTTGAATTGATCACTTT+TGG 0.305545 tig0023654:-43933 None:intergenic
CGCCAATGTATTAAATATCT+TGG 0.312762 tig0023654:+44823 Msa1410400:CDS
GTTTAAAACTGACCAACTTT+TGG 0.313670 tig0023654:-46213 None:intergenic
TCTGTTACTGATTTGTCAAA+TGG 0.323361 tig0023654:+43800 Msa1410400:CDS
TTGAATGTTGTATGCTTTAG+TGG 0.332350 tig0023654:+46374 Msa1410400:three_prime_UTR
GATGATACTTATTCAATTGT+TGG 0.336592 tig0023654:+45652 Msa1410400:CDS
ATAAATATTCAACTACTATA+TGG 0.341354 tig0023654:+43711 Msa1410400:five_prime_UTR
CTGCAAGTTAAAGGAAGCTT+TGG 0.359706 tig0023654:+46037 Msa1410400:exon
GCAATTTCCAAAGAATTTAG+AGG 0.365004 tig0023654:+46177 Msa1410400:three_prime_UTR
TGAATAAGTATCATCATCTA+TGG 0.366618 tig0023654:-45644 None:intergenic
GGTTGGAAAAGGTTTATCTA+AGG 0.378650 tig0023654:+44363 Msa1410400:intron
ATTGACCCGCGTCATCGATA+TGG 0.379869 tig0023654:+44547 Msa1410400:CDS
TTGTGGGGCATTATATTTAT+GGG 0.380913 tig0023654:+43773 Msa1410400:exon
AAAGGAAGCTTTGGAGGGAT+TGG 0.387195 tig0023654:+46046 Msa1410400:exon
CTGCAAGGTTTCTTCTAGTT+CGG 0.387260 tig0023654:-44066 None:intergenic
TTTAACTTGCAGTTGAAATT+GGG 0.397772 tig0023654:-46026 None:intergenic
GGCAGAGAAATCACTGGAAC+TGG 0.405732 tig0023654:-43959 None:intergenic
ATATGAAGTAATTGTTGAAA+AGG 0.407093 tig0023654:+44958 Msa1410400:CDS
TTGTCTTGCAAGTGGTCAAC+TGG 0.414943 tig0023654:+45808 Msa1410400:CDS
GCAGCATCAAGCTCAGAAAC+TGG 0.423052 tig0023654:-44010 None:intergenic
CTTTATCTTGAACAAATGAA+TGG 0.423444 tig0023654:-43892 None:intergenic
TGCAAGTGGTCAACTGGAGC+TGG 0.427085 tig0023654:+45814 Msa1410400:CDS
TCTTTGAAGTTCTCTTCTGT+TGG 0.432145 tig0023654:-45460 None:intergenic
GTCACCTAGGCTTGTGTACA+TGG 0.437362 tig0023654:+45984 Msa1410400:CDS
ATAATTTGTAGGTTGGATGT+TGG 0.439407 tig0023654:+44748 Msa1410400:intron
TGTGGGGCATTATATTTATG+GGG 0.448612 tig0023654:+43774 Msa1410400:exon
CAAGTTAAAGGAAGCTTTGG+AGG 0.449872 tig0023654:+46040 Msa1410400:exon
TATGAAGTAATTGTTGAAAA+GGG 0.453381 tig0023654:+44959 Msa1410400:CDS
TCAAGCTCAGAAACTGGTCT+TGG 0.453433 tig0023654:-44004 None:intergenic
AGCAAGAACAAGAGCAGCTA+AGG 0.455856 tig0023654:+44210 Msa1410400:CDS
CTGTTACTGATTTGTCAAAT+GGG 0.457364 tig0023654:+43801 Msa1410400:CDS
CATTCAAGCTTTCTTGCTGG+TGG 0.460779 tig0023654:+45103 Msa1410400:CDS
AATGATTTACCGTTGTTGTA+TGG 0.471270 tig0023654:-43510 None:intergenic
GTAGGAACCTTTGTTTGTTG+TGG 0.471273 tig0023654:-45703 None:intergenic
TGAAGTTCTCTTCTGTTGGA+TGG 0.474618 tig0023654:-45456 None:intergenic
CTAGGCCTAGGAGAAGGAAT+TGG 0.478665 tig0023654:-45946 None:intergenic
AGAGTTATGGTGGAAAGCAT+TGG 0.479204 tig0023654:+44108 Msa1410400:CDS
TCCGATAAAATCGATGGACT+TGG 0.484424 tig0023654:+46131 Msa1410400:three_prime_UTR
AGGTTTATCTAAGGATGAAA+AGG 0.485144 tig0023654:+44372 Msa1410400:CDS
TTATCATTGACATTGACCTT+AGG 0.486850 tig0023654:-45727 None:intergenic
AATTGTTGAAAAGGGAAGGT+TGG 0.496058 tig0023654:+44967 Msa1410400:CDS
CTAATGTTATGATTTACTAC+TGG 0.496264 tig0023654:-46269 None:intergenic
CAGTTGTAGTGGAAGAGTTA+TGG 0.497630 tig0023654:+44095 Msa1410400:CDS
ACTGGAGCTGGTCCACGTAT+TGG 0.499952 tig0023654:+45826 Msa1410400:CDS
ATACTAGGTGTCTAGGTCCT+TGG 0.502173 tig0023654:+46404 Msa1410400:three_prime_UTR
TGAAACTGCTGTGTCACGTT+GGG 0.503731 tig0023654:+44183 Msa1410400:CDS
TTGGTCCAATTCCTTCTCCT+AGG 0.504331 tig0023654:+45941 Msa1410400:CDS
AACAAGTTCATTTGTCTCCA+AGG 0.506581 tig0023654:+45893 Msa1410400:CDS
GTCCAAGATATTTAATACAT+TGG 0.512159 tig0023654:-44825 None:intergenic
GTACTGATGAGAAATCAAAA+TGG 0.515735 tig0023654:+45017 Msa1410400:CDS
ATTGATCGTACAGTTATTGG+AGG 0.515941 tig0023654:+43594 Msa1410400:five_prime_UTR
GGATGGTAGTGTCCACTGTA+TGG 0.524176 tig0023654:-45439 None:intergenic
TCATTCTTCAAACCAAAAGT+TGG 0.526454 tig0023654:+46201 Msa1410400:three_prime_UTR
TGTAGGTTGGATGTTGGAGA+TGG 0.537642 tig0023654:+44754 Msa1410400:intron
GAAATTGGGTTTCTTGGACT+AGG 0.538092 tig0023654:-46012 None:intergenic
ACACTTGCAATCAAAAGCAT+TGG 0.539552 tig0023654:+45870 Msa1410400:CDS
ATTCTTTGGAAATTGCTCTA+CGG 0.540770 tig0023654:-46170 None:intergenic
GTGGGGCATTATATTTATGG+GGG 0.541213 tig0023654:+43775 Msa1410400:exon
GGTGGTGCAACCACAGCAGC+TGG 0.542342 tig0023654:+45121 Msa1410400:CDS
CACTGCAAGAGCCTTGTATG+TGG 0.543226 tig0023654:+45054 Msa1410400:CDS
AAGTAATTGTTGAAAAGGGA+AGG 0.544030 tig0023654:+44963 Msa1410400:CDS
AACAAAGGTTCCTACTCCTA+AGG 0.545400 tig0023654:+45711 Msa1410400:CDS
TTGCAGCATTTAACGCCTCA+AGG 0.550558 tig0023654:+45782 Msa1410400:CDS
CCGATAAAATCGATGGACTT+GGG 0.551665 tig0023654:+46132 Msa1410400:three_prime_UTR
TTTAGTGGTACATACATACT+AGG 0.560086 tig0023654:+46389 Msa1410400:three_prime_UTR
TGTAGACCTCACAAATGTAA+AGG 0.560440 tig0023654:+45510 Msa1410400:CDS
TATTTACCTTTACATTTGTG+AGG 0.561561 tig0023654:-45516 None:intergenic
TGCAGATTGTGCAGTTGTAG+TGG 0.569563 tig0023654:+44084 Msa1410400:CDS
CAATTTCCAAAGAATTTAGA+GGG 0.574393 tig0023654:+46178 Msa1410400:three_prime_UTR
CTGCAAGAGCCTTGTATGTG+GGG 0.574800 tig0023654:+45056 Msa1410400:CDS
AATCCCATGTACACAAGCCT+AGG 0.574805 tig0023654:-45988 None:intergenic
GGTAGATTAGTAGCAAATCA+AGG 0.575049 tig0023654:+45142 Msa1410400:CDS
TTTGGGCTAGGCCTAGGAGA+AGG 0.580187 tig0023654:-45952 None:intergenic
ATACAATCCGATAAAATCGA+TGG 0.580324 tig0023654:+46125 Msa1410400:three_prime_UTR
AAGAAAGCTTGAATGTTGAA+AGG 0.583819 tig0023654:-45095 None:intergenic
TATTCAACTACTATATGGCA+TGG 0.584888 tig0023654:+43716 Msa1410400:five_prime_UTR
GCTTCAGGCAGAGAAATCAC+TGG 0.585648 tig0023654:-43965 None:intergenic
TACTAATCTACCAGCTGCTG+TGG 0.585907 tig0023654:-45131 None:intergenic
ACTGCAAGAGCCTTGTATGT+GGG 0.586114 tig0023654:+45055 Msa1410400:CDS
TACATACATACTAGGTGTCT+AGG 0.589102 tig0023654:+46397 Msa1410400:three_prime_UTR
TTGTAGTGGAAGAGTTATGG+TGG 0.592352 tig0023654:+44098 Msa1410400:CDS
AAGTTAAAGGAAGCTTTGGA+GGG 0.593505 tig0023654:+46041 Msa1410400:exon
GGTTGGTGTATAAACAAGAC+GGG 0.595499 tig0023654:+44984 Msa1410400:CDS
CAATGAACCACAACAAACAA+AGG 0.595908 tig0023654:+45696 Msa1410400:CDS
CTTTCAAGAATCATATGCTG+AGG 0.598146 tig0023654:-43866 None:intergenic
CTGAAACTGCTGTGTCACGT+TGG 0.601275 tig0023654:+44182 Msa1410400:CDS
AGTATCCTCTGTTGTGACCA+TGG 0.604033 tig0023654:-46235 None:intergenic
TCAAGATAAAGAACAAAACA+TGG 0.608091 tig0023654:+43904 Msa1410400:CDS
TTGACATTGACCTTAGGAGT+AGG 0.608674 tig0023654:-45721 None:intergenic
CTACAACTGCACAATCTGCA+AGG 0.616966 tig0023654:-44081 None:intergenic
ATAAGATGAGTACTTCAAGC+AGG 0.620905 tig0023654:+43828 Msa1410400:CDS
ATGGACTTGGGAGCAATCTG+AGG 0.632608 tig0023654:+46144 Msa1410400:three_prime_UTR
CAAGGAGGTTGTCTTGCAAG+TGG 0.637929 tig0023654:+45800 Msa1410400:CDS
ACAGAGGATACTTCATGTGA+CGG 0.638711 tig0023654:+46246 Msa1410400:three_prime_UTR
AGCAAATCAAGGTGTTCTTG+AGG 0.643990 tig0023654:+45153 Msa1410400:CDS
TCACCTAGGCTTGTGTACAT+GGG 0.646732 tig0023654:+45985 Msa1410400:CDS
AGGTTGGTGTATAAACAAGA+CGG 0.647522 tig0023654:+44983 Msa1410400:CDS
TTAAACCATGGTCACAACAG+AGG 0.650726 tig0023654:+46230 Msa1410400:three_prime_UTR
GACTTGGGAGCAATCTGAGG+AGG 0.653305 tig0023654:+46147 Msa1410400:three_prime_UTR
CAAAAGTTAGAATGTCACCT+AGG 0.655633 tig0023654:+45971 Msa1410400:CDS
TTGTGTCCATATCGATGACG+CGG 0.659774 tig0023654:-44553 None:intergenic
CAGGCTATTTGGCCATACAG+TGG 0.663221 tig0023654:+45427 Msa1410400:intron
CTTGCAAGACAACCTCCTTG+AGG 0.665708 tig0023654:-45797 None:intergenic
TCACGAACACAACCAATACG+TGG 0.684822 tig0023654:-45838 None:intergenic
TGTGTCCATATCGATGACGC+GGG 0.693829 tig0023654:-44552 None:intergenic
ATTCATACACCATACAACAA+CGG 0.701420 tig0023654:+43501 Msa1410400:five_prime_UTR
TGGTGTATAAACAAGACGGG+AGG 0.718519 tig0023654:+44987 Msa1410400:CDS
CAGCATTTAACGCCTCAAGG+AGG 0.731550 tig0023654:+45785 Msa1410400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATGATAAATAATTTGT+AGG + tig0023654:44737-44756 Msa1410400:intron 10.0%
!! AACAAATTAATGCAATTATT+TGG + tig0023654:43670-43689 Msa1410400:five_prime_UTR 15.0%
!! ATAAATATTCAACTACTATA+TGG + tig0023654:43711-43730 Msa1410400:five_prime_UTR 15.0%
!! ATTTATAGAAAGAGTTTAAT+TGG - tig0023654:45358-45377 None:intergenic 15.0%
!!! GTTTATTACTTCATTTTTTT+AGG + tig0023654:46348-46367 Msa1410400:three_prime_UTR 15.0%
!!! CTTATTCATGTTACTTTATA+TGG + tig0023654:44294-44313 Msa1410400:intron 20.0%
!!! ACTTTTTATAGTTTTTGATC+TGG + tig0023654:44489-44508 Msa1410400:intron 20.0%
!!! ATGATAAATAATTTGTAGGT+TGG + tig0023654:44741-44760 Msa1410400:intron 20.0%
!! AAGATAAACAATTATGAGAT+AGG - tig0023654:44905-44924 None:intergenic 20.0%
!! ATATGAAGTAATTGTTGAAA+AGG + tig0023654:44958-44977 Msa1410400:CDS 20.0%
!! TATGAAGTAATTGTTGAAAA+GGG + tig0023654:44959-44978 Msa1410400:CDS 20.0%
!!! TATTTTCAAAGTTATCTATG+TGG + tig0023654:45300-45319 Msa1410400:intron 20.0%
!! AAAGAATTTATAAGCTTTCT+TGG + tig0023654:45478-45497 Msa1410400:CDS 20.0%
!! TTGATCCTCAAAATAATTAT+TGG + tig0023654:45540-45559 Msa1410400:intron 20.0%
!!! TACTACCAATAATTATTTTG+AGG - tig0023654:45548-45567 None:intergenic 20.0%
!! ATTTCATTAGTACTTTACAT+AGG - tig0023654:45578-45597 None:intergenic 20.0%
! TTAATTGATCGTACAGTTAT+TGG + tig0023654:43591-43610 Msa1410400:five_prime_UTR 25.0%
!! TATAATTTTGTGCAGATTTG+TGG + tig0023654:43756-43775 Msa1410400:five_prime_UTR 25.0%
!! ATAATTTTGTGCAGATTTGT+GGG + tig0023654:43757-43776 Msa1410400:five_prime_UTR 25.0%
! CTTTATCTTGAACAAATGAA+TGG - tig0023654:43895-43914 None:intergenic 25.0%
! TCAAGATAAAGAACAAAACA+TGG + tig0023654:43904-43923 Msa1410400:CDS 25.0%
!! ATGTTTGAATTGATCACTTT+TGG - tig0023654:43936-43955 None:intergenic 25.0%
! ACCTTTACAAAAAAGAGTTA+CGG - tig0023654:44346-44365 None:intergenic 25.0%
!!! TAACTCTTTTTTGTAAAGGT+TGG + tig0023654:44346-44365 Msa1410400:intron 25.0%
!!! TTTTTTGTAAAGGTTGGAAA+AGG + tig0023654:44352-44371 Msa1410400:intron 25.0%
! AAAAATTAGCACTTCAACAT+TGG + tig0023654:44398-44417 Msa1410400:CDS 25.0%
! TTCACATGTATTATGATATC+TGG + tig0023654:44578-44597 Msa1410400:CDS 25.0%
! GTCCAAGATATTTAATACAT+TGG - tig0023654:44828-44847 None:intergenic 25.0%
!! TATCTACATGATCTCAATTT+TGG + tig0023654:44856-44875 Msa1410400:intron 25.0%
! ATTAAATCCTTATGAAAAGC+TGG - tig0023654:45199-45218 None:intergenic 25.0%
! TATCCACTAAATAAAAATGC+AGG + tig0023654:45408-45427 Msa1410400:intron 25.0%
! AAATAAAAATGCAGGCTATT+TGG + tig0023654:45416-45435 Msa1410400:intron 25.0%
! TATTTACCTTTACATTTGTG+AGG - tig0023654:45519-45538 None:intergenic 25.0%
! TGAATAAGTATCATCATCTA+TGG - tig0023654:45647-45666 None:intergenic 25.0%
! GATGATACTTATTCAATTGT+TGG + tig0023654:45652-45671 Msa1410400:CDS 25.0%
!!! TTTTCAAGACTCAACAAATT+TGG + tig0023654:45922-45941 Msa1410400:CDS 25.0%
!!! TTTAACTTGCAGTTGAAATT+GGG - tig0023654:46029-46048 None:intergenic 25.0%
! CAATTTCCAAAGAATTTAGA+GGG + tig0023654:46178-46197 Msa1410400:three_prime_UTR 25.0%
! CTAATGTTATGATTTACTAC+TGG - tig0023654:46272-46291 None:intergenic 25.0%
! ATTGTCCAAAAAAATGAGAA+GGG - tig0023654:46312-46331 None:intergenic 25.0%
! AATTGTCCAAAAAAATGAGA+AGG - tig0023654:46313-46332 None:intergenic 25.0%
! AAAAGAAAGAGGCAAAAATA+GGG - tig0023654:46430-46449 None:intergenic 25.0%
! AAAAAGAAAGAGGCAAAAAT+AGG - tig0023654:46431-46450 None:intergenic 25.0%
ATTCATACACCATACAACAA+CGG + tig0023654:43501-43520 Msa1410400:five_prime_UTR 30.0%
AATGATTTACCGTTGTTGTA+TGG - tig0023654:43513-43532 None:intergenic 30.0%
CAGTTATTGGAGGTTAATTA+TGG + tig0023654:43604-43623 Msa1410400:five_prime_UTR 30.0%
TATTCAACTACTATATGGCA+TGG + tig0023654:43716-43735 Msa1410400:five_prime_UTR 30.0%
! TAATTTTGTGCAGATTTGTG+GGG + tig0023654:43758-43777 Msa1410400:five_prime_UTR 30.0%
! TTTGTGGGGCATTATATTTA+TGG + tig0023654:43772-43791 Msa1410400:exon 30.0%
! TTGTGGGGCATTATATTTAT+GGG + tig0023654:43773-43792 Msa1410400:exon 30.0%
!! TCTGTTACTGATTTGTCAAA+TGG + tig0023654:43800-43819 Msa1410400:CDS 30.0%
!! CTGTTACTGATTTGTCAAAT+GGG + tig0023654:43801-43820 Msa1410400:CDS 30.0%
!!! ACCGTAACTCTTTTTTGTAA+AGG + tig0023654:44342-44361 Msa1410400:intron 30.0%
AGGTTTATCTAAGGATGAAA+AGG + tig0023654:44372-44391 Msa1410400:CDS 30.0%
AGAGTTTACCAGTAGAAAAA+TGG - tig0023654:44625-44644 None:intergenic 30.0%
ACAGATGAAGATTGTAAAAC+AGG + tig0023654:44682-44701 Msa1410400:intron 30.0%
! ATAATTTGTAGGTTGGATGT+TGG + tig0023654:44748-44767 Msa1410400:intron 30.0%
CGCCAATGTATTAAATATCT+TGG + tig0023654:44823-44842 Msa1410400:CDS 30.0%
AGATCATGTAGATAACTTAC+TGG - tig0023654:44850-44869 None:intergenic 30.0%
AAGTAATTGTTGAAAAGGGA+AGG + tig0023654:44963-44982 Msa1410400:CDS 30.0%
! GTACTGATGAGAAATCAAAA+TGG + tig0023654:45017-45036 Msa1410400:CDS 30.0%
AAGAAAGCTTGAATGTTGAA+AGG - tig0023654:45098-45117 None:intergenic 30.0%
!!! GTGTGTGTAAAACTTTTTTC+AGG - tig0023654:45231-45250 None:intergenic 30.0%
!! TAGCCTGCATTTTTATTTAG+TGG - tig0023654:45414-45433 None:intergenic 30.0%
TTATCATTGACATTGACCTT+AGG - tig0023654:45730-45749 None:intergenic 30.0%
TGTGACATCAACTCATAAAA+AGG + tig0023654:45753-45772 Msa1410400:CDS 30.0%
TTGTTGAGTCTTGAAAAACT+TGG - tig0023654:45919-45938 None:intergenic 30.0%
! TAGGTGACATTCTAACTTTT+GGG - tig0023654:45972-45991 None:intergenic 30.0%
!! CTTTAACTTGCAGTTGAAAT+TGG - tig0023654:46030-46049 None:intergenic 30.0%
CAATTTCAACTGCAAGTTAA+AGG + tig0023654:46028-46047 Msa1410400:CDS 30.0%
ATACAATCCGATAAAATCGA+TGG + tig0023654:46125-46144 Msa1410400:three_prime_UTR 30.0%
! ATTCTTTGGAAATTGCTCTA+CGG - tig0023654:46173-46192 None:intergenic 30.0%
GCAATTTCCAAAGAATTTAG+AGG + tig0023654:46177-46196 Msa1410400:three_prime_UTR 30.0%
TCATTCTTCAAACCAAAAGT+TGG + tig0023654:46201-46220 Msa1410400:three_prime_UTR 30.0%
!! GTTTAAAACTGACCAACTTT+TGG - tig0023654:46216-46235 None:intergenic 30.0%
!!! AATTCCCCTTCTCATTTTTT+TGG + tig0023654:46304-46323 Msa1410400:three_prime_UTR 30.0%
TTGTCCAAAAAAATGAGAAG+GGG - tig0023654:46311-46330 None:intergenic 30.0%
TTGAATGTTGTATGCTTTAG+TGG + tig0023654:46374-46393 Msa1410400:three_prime_UTR 30.0%
TTTAGTGGTACATACATACT+AGG + tig0023654:46389-46408 Msa1410400:three_prime_UTR 30.0%
! TTTTTGCCTCTTTCTTTTTC+TGG + tig0023654:46432-46451 Msa1410400:three_prime_UTR 30.0%
ATTGATCGTACAGTTATTGG+AGG + tig0023654:43594-43613 Msa1410400:five_prime_UTR 35.0%
! TGTGGGGCATTATATTTATG+GGG + tig0023654:43774-43793 Msa1410400:exon 35.0%
! GCTTGAAGTACTCATCTTAT+TGG - tig0023654:43828-43847 None:intergenic 35.0%
ATAAGATGAGTACTTCAAGC+AGG + tig0023654:43828-43847 Msa1410400:CDS 35.0%
CTTTCAAGAATCATATGCTG+AGG - tig0023654:43869-43888 None:intergenic 35.0%
GAAGAAAAAACAACTGCTTC+AGG - tig0023654:43983-44002 None:intergenic 35.0%
GGTTGGAAAAGGTTTATCTA+AGG + tig0023654:44363-44382 Msa1410400:intron 35.0%
!! GTTCACAACCATTTTTCTAC+TGG + tig0023654:44614-44633 Msa1410400:CDS 35.0%
! AGGAGCTAAAGTGTTCTATA+TGG + tig0023654:44702-44721 Msa1410400:intron 35.0%
AATTGTTGAAAAGGGAAGGT+TGG + tig0023654:44967-44986 Msa1410400:CDS 35.0%
!! AGGTTGGTGTATAAACAAGA+CGG + tig0023654:44983-45002 Msa1410400:CDS 35.0%
TATGTGGGGAAAAAGCAAAA+GGG + tig0023654:45070-45089 Msa1410400:CDS 35.0%
GGTAGATTAGTAGCAAATCA+AGG + tig0023654:45142-45161 Msa1410400:CDS 35.0%
!! TCTTTGAAGTTCTCTTCTGT+TGG - tig0023654:45463-45482 None:intergenic 35.0%
TGTAGACCTCACAAATGTAA+AGG + tig0023654:45510-45529 Msa1410400:CDS 35.0%
CAATGAACCACAACAAACAA+AGG + tig0023654:45696-45715 Msa1410400:CDS 35.0%
! ACACTTGCAATCAAAAGCAT+TGG + tig0023654:45870-45889 Msa1410400:CDS 35.0%
AACAAGTTCATTTGTCTCCA+AGG + tig0023654:45893-45912 Msa1410400:CDS 35.0%
! CTAGGTGACATTCTAACTTT+TGG - tig0023654:45973-45992 None:intergenic 35.0%
CAAAAGTTAGAATGTCACCT+AGG + tig0023654:45971-45990 Msa1410400:CDS 35.0%
!! AAGTTAAAGGAAGCTTTGGA+GGG + tig0023654:46041-46060 Msa1410400:exon 35.0%
GAATGACCCTCTAAATTCTT+TGG - tig0023654:46187-46206 None:intergenic 35.0%
!!! AGTTGGTCAGTTTTAAACCA+TGG + tig0023654:46218-46237 Msa1410400:three_prime_UTR 35.0%
TACATACATACTAGGTGTCT+AGG + tig0023654:46397-46416 Msa1410400:three_prime_UTR 35.0%
! GTGGGGCATTATATTTATGG+GGG + tig0023654:43775-43794 Msa1410400:exon 40.0%
! CTGCAAGGTTTCTTCTAGTT+CGG - tig0023654:44069-44088 None:intergenic 40.0%
! CAGTTGTAGTGGAAGAGTTA+TGG + tig0023654:44095-44114 Msa1410400:CDS 40.0%
! TTGTAGTGGAAGAGTTATGG+TGG + tig0023654:44098-44117 Msa1410400:CDS 40.0%
! AGAGTTATGGTGGAAAGCAT+TGG + tig0023654:44108-44127 Msa1410400:CDS 40.0%
! GGTTGGTGTATAAACAAGAC+GGG + tig0023654:44984-45003 Msa1410400:CDS 40.0%
!!! TTGCTTTTTCCCCACATACA+AGG - tig0023654:45068-45087 None:intergenic 40.0%
GTATGTGGGGAAAAAGCAAA+AGG + tig0023654:45069-45088 Msa1410400:CDS 40.0%
CAACATTCAAGCTTTCTTGC+TGG + tig0023654:45100-45119 Msa1410400:CDS 40.0%
! AGCAAATCAAGGTGTTCTTG+AGG + tig0023654:45153-45172 Msa1410400:CDS 40.0%
TGAAAAGCTGGAGTGGAATT+TGG - tig0023654:45187-45206 None:intergenic 40.0%
! TTCCACTCCAGCTTTTCATA+AGG + tig0023654:45189-45208 Msa1410400:intron 40.0%
ATCCTTATGAAAAGCTGGAG+TGG - tig0023654:45194-45213 None:intergenic 40.0%
TGAAGTTCTCTTCTGTTGGA+TGG - tig0023654:45459-45478 None:intergenic 40.0%
! GTAGGAACCTTTGTTTGTTG+TGG - tig0023654:45706-45725 None:intergenic 40.0%
AACAAAGGTTCCTACTCCTA+AGG + tig0023654:45711-45730 Msa1410400:CDS 40.0%
TTGACATTGACCTTAGGAGT+AGG - tig0023654:45724-45743 None:intergenic 40.0%
!! GCTTTTGATTGCAAGTGTTC+AGG - tig0023654:45868-45887 None:intergenic 40.0%
AGTCTTGAAAAACTTGGCCT+TGG - tig0023654:45913-45932 None:intergenic 40.0%
! GACATTCTAACTTTTGGGCT+AGG - tig0023654:45967-45986 None:intergenic 40.0%
GAAATTGGGTTTCTTGGACT+AGG - tig0023654:46015-46034 None:intergenic 40.0%
GCAGTTGAAATTGGGTTTCT+TGG - tig0023654:46021-46040 None:intergenic 40.0%
CTGCAAGTTAAAGGAAGCTT+TGG + tig0023654:46037-46056 Msa1410400:exon 40.0%
! CAAGTTAAAGGAAGCTTTGG+AGG + tig0023654:46040-46059 Msa1410400:exon 40.0%
! TCCGATAAAATCGATGGACT+TGG + tig0023654:46131-46150 Msa1410400:three_prime_UTR 40.0%
!!! CCCAAGTCCATCGATTTTAT+CGG - tig0023654:46135-46154 None:intergenic 40.0%
! CCGATAAAATCGATGGACTT+GGG + tig0023654:46132-46151 Msa1410400:three_prime_UTR 40.0%
TTAAACCATGGTCACAACAG+AGG + tig0023654:46230-46249 Msa1410400:three_prime_UTR 40.0%
ACAGAGGATACTTCATGTGA+CGG + tig0023654:46246-46265 Msa1410400:three_prime_UTR 40.0%
! TGCCTCTTTCTTTTTCTGGA+AGG + tig0023654:46436-46455 Msa1410400:three_prime_UTR 40.0%
TGCCTTCCAGAAAAAGAAAG+AGG - tig0023654:46441-46460 None:intergenic 40.0%
! TCAAGCTCAGAAACTGGTCT+TGG - tig0023654:44007-44026 None:intergenic 45.0%
CTACAACTGCACAATCTGCA+AGG - tig0023654:44084-44103 None:intergenic 45.0%
TGCAGATTGTGCAGTTGTAG+TGG + tig0023654:44084-44103 Msa1410400:CDS 45.0%
TGAAACTGCTGTGTCACGTT+GGG + tig0023654:44183-44202 Msa1410400:CDS 45.0%
AGCAAGAACAAGAGCAGCTA+AGG + tig0023654:44210-44229 Msa1410400:CDS 45.0%
TTGTGTCCATATCGATGACG+CGG - tig0023654:44556-44575 None:intergenic 45.0%
! TGTAGGTTGGATGTTGGAGA+TGG + tig0023654:44754-44773 Msa1410400:intron 45.0%
CGTTGTAGAACAGTGCTTCT+TGG - tig0023654:44805-44824 None:intergenic 45.0%
TGGTGTATAAACAAGACGGG+AGG + tig0023654:44987-45006 Msa1410400:CDS 45.0%
! ACTGCAAGAGCCTTGTATGT+GGG + tig0023654:45055-45074 Msa1410400:CDS 45.0%
CATTCAAGCTTTCTTGCTGG+TGG + tig0023654:45103-45122 Msa1410400:CDS 45.0%
TACTAATCTACCAGCTGCTG+TGG - tig0023654:45134-45153 None:intergenic 45.0%
CATCATCTATGGCACATCTC+TGG - tig0023654:45636-45655 None:intergenic 45.0%
TTGCAGCATTTAACGCCTCA+AGG + tig0023654:45782-45801 Msa1410400:CDS 45.0%
TTGTCTTGCAAGTGGTCAAC+TGG + tig0023654:45808-45827 Msa1410400:CDS 45.0%
TCACGAACACAACCAATACG+TGG - tig0023654:45841-45860 None:intergenic 45.0%
TTGGTCCAATTCCTTCTCCT+AGG + tig0023654:45941-45960 Msa1410400:CDS 45.0%
TCACCTAGGCTTGTGTACAT+GGG + tig0023654:45985-46004 Msa1410400:CDS 45.0%
AATCCCATGTACACAAGCCT+AGG - tig0023654:45991-46010 None:intergenic 45.0%
!! AAAGGAAGCTTTGGAGGGAT+TGG + tig0023654:46046-46065 Msa1410400:exon 45.0%
!!! GAGGGATTGGTTTTTGCTCA+GGG + tig0023654:46059-46078 Msa1410400:three_prime_UTR 45.0%
AGTATCCTCTGTTGTGACCA+TGG - tig0023654:46238-46257 None:intergenic 45.0%
ATACTAGGTGTCTAGGTCCT+TGG + tig0023654:46404-46423 Msa1410400:three_prime_UTR 45.0%
AAGAGGCAAAAATAGGGCCA+AGG - tig0023654:46424-46443 None:intergenic 45.0%
!! TGAATATTTATTATATATTT+GGG - tig0023654:43702-43721 None:intergenic 5.0%
!! TTGAATATTTATTATATATT+TGG - tig0023654:43703-43722 None:intergenic 5.0%
GGCAGAGAAATCACTGGAAC+TGG - tig0023654:43962-43981 None:intergenic 50.0%
GCTTCAGGCAGAGAAATCAC+TGG - tig0023654:43968-43987 None:intergenic 50.0%
GCAGCATCAAGCTCAGAAAC+TGG - tig0023654:44013-44032 None:intergenic 50.0%
CAACGTGACACAGCAGTTTC+AGG - tig0023654:44184-44203 None:intergenic 50.0%
CTGAAACTGCTGTGTCACGT+TGG + tig0023654:44182-44201 Msa1410400:CDS 50.0%
ATTGACCCGCGTCATCGATA+TGG + tig0023654:44547-44566 Msa1410400:CDS 50.0%
TGTGTCCATATCGATGACGC+GGG - tig0023654:44555-44574 None:intergenic 50.0%
! CACTGCAAGAGCCTTGTATG+TGG + tig0023654:45054-45073 Msa1410400:CDS 50.0%
! CTGCAAGAGCCTTGTATGTG+GGG + tig0023654:45056-45075 Msa1410400:CDS 50.0%
CAGGCTATTTGGCCATACAG+TGG + tig0023654:45427-45446 Msa1410400:intron 50.0%
! GGATGGTAGTGTCCACTGTA+TGG - tig0023654:45442-45461 None:intergenic 50.0%
CAGCATTTAACGCCTCAAGG+AGG + tig0023654:45785-45804 Msa1410400:CDS 50.0%
CTTGCAAGACAACCTCCTTG+AGG - tig0023654:45800-45819 None:intergenic 50.0%
CAAGGAGGTTGTCTTGCAAG+TGG + tig0023654:45800-45819 Msa1410400:CDS 50.0%
CTAGGCCTAGGAGAAGGAAT+TGG - tig0023654:45949-45968 None:intergenic 50.0%
!! CTAACTTTTGGGCTAGGCCT+AGG - tig0023654:45961-45980 None:intergenic 50.0%
GTCACCTAGGCTTGTGTACA+TGG + tig0023654:45984-46003 Msa1410400:CDS 50.0%
!!! GGAGGGATTGGTTTTTGCTC+AGG + tig0023654:46058-46077 Msa1410400:three_prime_UTR 50.0%
ATGGACTTGGGAGCAATCTG+AGG + tig0023654:46144-46163 Msa1410400:three_prime_UTR 50.0%
TGCAAGTGGTCAACTGGAGC+TGG + tig0023654:45814-45833 Msa1410400:CDS 55.0%
ACTGGAGCTGGTCCACGTAT+TGG + tig0023654:45826-45845 Msa1410400:CDS 55.0%
! TTTGGGCTAGGCCTAGGAGA+AGG - tig0023654:45955-45974 None:intergenic 55.0%
GACTTGGGAGCAATCTGAGG+AGG + tig0023654:46147-46166 Msa1410400:three_prime_UTR 55.0%
GGTGGTGCAACCACAGCAGC+TGG + tig0023654:45121-45140 Msa1410400:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0023654 gene 43410 46534 43410 ID=Msa1410400;Name=Msa1410400
tig0023654 mRNA 43410 46534 43410 ID=Msa1410400-mRNA-1;Parent=Msa1410400;Name=Msa1410400-mRNA-1;_AED=0.08;_eAED=0.08;_QI=326|1|1|1|0.85|0.75|8|487|476
tig0023654 exon 43410 43522 43410 ID=Msa1410400-mRNA-1:exon:26514;Parent=Msa1410400-mRNA-1
tig0023654 exon 43578 44231 43578 ID=Msa1410400-mRNA-1:exon:26515;Parent=Msa1410400-mRNA-1
tig0023654 exon 44364 44429 44364 ID=Msa1410400-mRNA-1:exon:26516;Parent=Msa1410400-mRNA-1
tig0023654 exon 44547 44635 44547 ID=Msa1410400-mRNA-1:exon:26517;Parent=Msa1410400-mRNA-1
tig0023654 exon 44759 44849 44759 ID=Msa1410400-mRNA-1:exon:26518;Parent=Msa1410400-mRNA-1
tig0023654 exon 44944 45174 44944 ID=Msa1410400-mRNA-1:exon:26519;Parent=Msa1410400-mRNA-1
tig0023654 exon 45430 45531 45430 ID=Msa1410400-mRNA-1:exon:26520;Parent=Msa1410400-mRNA-1
tig0023654 exon 45637 46534 45637 ID=Msa1410400-mRNA-1:exon:26521;Parent=Msa1410400-mRNA-1
tig0023654 five_prime_UTR 43410 43522 43410 ID=Msa1410400-mRNA-1:five_prime_utr;Parent=Msa1410400-mRNA-1
tig0023654 five_prime_UTR 43578 43790 43578 ID=Msa1410400-mRNA-1:five_prime_utr;Parent=Msa1410400-mRNA-1
tig0023654 CDS 43791 44231 43791 ID=Msa1410400-mRNA-1:cds;Parent=Msa1410400-mRNA-1
tig0023654 CDS 44364 44429 44364 ID=Msa1410400-mRNA-1:cds;Parent=Msa1410400-mRNA-1
tig0023654 CDS 44547 44635 44547 ID=Msa1410400-mRNA-1:cds;Parent=Msa1410400-mRNA-1
tig0023654 CDS 44759 44849 44759 ID=Msa1410400-mRNA-1:cds;Parent=Msa1410400-mRNA-1
tig0023654 CDS 44944 45174 44944 ID=Msa1410400-mRNA-1:cds;Parent=Msa1410400-mRNA-1
tig0023654 CDS 45430 45531 45430 ID=Msa1410400-mRNA-1:cds;Parent=Msa1410400-mRNA-1
tig0023654 CDS 45637 46047 45637 ID=Msa1410400-mRNA-1:cds;Parent=Msa1410400-mRNA-1
tig0023654 three_prime_UTR 46048 46534 46048 ID=Msa1410400-mRNA-1:three_prime_utr;Parent=Msa1410400-mRNA-1
Gene Sequence

>Msa1410400

ATGGGGGTGTCTGTTACTGATTTGTCAAATGGGTCCAATAAGATGAGTACTTCAAGCAGGTTGAGTGATTGCAGACCTCAGCATATGATTCTTGAAAGAAACCATTCATTTGTTCAAGATAAAGAACAAAACATGGATTCAACCAAAAGTGATCAATTCAAACATAAACCAGTTCCAGTGATTTCTCTGCCTGAAGCAGTTGTTTTTTCTTCTCCAAGACCAGTTTCTGAGCTTGATGCTGCTGCAACTACACTTCAAAAAGTATACAAGAGTTACCGAACTAGAAGAAACCTTGCAGATTGTGCAGTTGTAGTGGAAGAGTTATGGTGGAAAGCATTGGATTTTGCTGCTCTTAGAAGAAGTTCTGTTTCATTCTTTGATGTTGAGAAACCTGAAACTGCTGTGTCACGTTGGGCTAGAGCAAGAACAAGAGCAGCTAAGGTTGGAAAAGGTTTATCTAAGGATGAAAAGGCACAAAAATTAGCACTTCAACATTGGCTTGAAGCTATTGACCCGCGTCATCGATATGGACACAATCTTCACATGTATTATGATATCTGGTTTAAAAGCTTAAGTTCACAACCATTTTTCTACTGGTTGGATGTTGGAGATGGAAAAGAAATAAATCTTGAGAAATGTCCAAGAAGCACTGTTCTACAACGCCAATGTATTAAATATCTTGGACCAAAAGAAAGAGAAGAATATGAAGTAATTGTTGAAAAGGGAAGGTTGGTGTATAAACAAGACGGGAGGCTTGTGAGTACTGATGAGAAATCAAAATGGATATTTGTTCTCAGCACTGCAAGAGCCTTGTATGTGGGGAAAAAGCAAAAGGGTGCCTTTCAACATTCAAGCTTTCTTGCTGGTGGTGCAACCACAGCAGCTGGTAGATTAGTAGCAAATCAAGGTGTTCTTGAGGCTATTTGGCCATACAGTGGACACTACCATCCAACAGAAGAGAACTTCAAAGAATTTATAAGCTTTCTTGGAGAGCACGATGTAGACCTCACAAATGTAAAGAGATGTGCCATAGATGATGATACTTATTCAATTGTTGGAAATGATTCATTTGATGTCATCAATGAACCACAACAAACAAAGGTTCCTACTCCTAAGGTCAATGTCAATGATAACAATGTGACATCAACTCATAAAAAGGATGCTGTTGCAGCATTTAACGCCTCAAGGAGGTTGTCTTGCAAGTGGTCAACTGGAGCTGGTCCACGTATTGGTTGTGTTCGTGACTATCCTGAACACTTGCAATCAAAAGCATTGGAACAAGTTCATTTGTCTCCAAGGCCAAGTTTTTCAAGACTCAACAAATTTGGTCCAATTCCTTCTCCTAGGCCTAGCCCAAAAGTTAGAATGTCACCTAGGCTTGTGTACATGGGATTGCCTAGTCCAAGAAACCCAATTTCAACTGCAAGTTAA
Protein sequence

>Msa1410400

MGVSVTDLSNGSNKMSTSSRLSDCRPQHMILERNHSFVQDKEQNMDSTKSDQFKHKPVPVISLPEAVVFSSPRPVSELDAAATTLQKVYKSYRTRRNLADCAVVVEELWWKALDFAALRRSSVSFFDVEKPETAVSRWARARTRAAKVGKGLSKDEKAQKLALQHWLEAIDPRHRYGHNLHMYYDIWFKSLSSQPFFYWLDVGDGKEINLEKCPRSTVLQRQCIKYLGPKEREEYEVIVEKGRLVYKQDGRLVSTDEKSKWIFVLSTARALYVGKKQKGAFQHSSFLAGGATTAAGRLVANQGVLEAIWPYSGHYHPTEENFKEFISFLGEHDVDLTNVKRCAIDDDTYSIVGNDSFDVINEPQQTKVPTPKVNVNDNNVTSTHKKDAVAAFNASRRLSCKWSTGAGPRIGCVRDYPEHLQSKALEQVHLSPRPSFSRLNKFGPIPSPRPSPKVRMSPRLVYMGLPSPRNPISTAS*