AlfalfaGEDB | Msa1410400

M. sativa cultivar zhongmu-4 / Msa1410400

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0008150 GO:0010119 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0065007
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1410400	sp\|O82645\|IQM1_ARATH	69.586	411	112	3	59	469	87	484	0.0	579

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1410400	A0A072US72	97.059	476	11	1	1	476	1	473	0.0	960

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0697590	Msa1410400	0.800698	1.275863e-48	-8.615850e-47
Msa1260680	Msa1410400	0.804007	2.655968e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1410400	MtrunA17_Chr4g0071041	97.059	476	11	1	1	476	1	473	0.0	960
Msa1410400	MtrunA17_Chr2g0299641	74.839	465	90	5	20	472	57	506	0.0	710
Msa1410400	MtrunA17_Chr8g0342561	64.169	427	146	3	51	476	84	504	0.0	557
Msa1410400	MtrunA17_Chr2g0330061	56.618	408	148	6	64	465	80	464	1.14e-152	442
Msa1410400	MtrunA17_Chr8g0378761	60.172	349	123	5	9	346	55	398	3.37e-142	422
Msa1410400	MtrunA17_Chr8g0378761	44.706	85	34	3	359	433	513	594	1.52e-12	69.7
Msa1410400	MtrunA17_Chr3g0121261	68.100	279	81	3	85	356	6	283	5.43e-139	401
Msa1410400	MtrunA17_Chr1g0176231	69.343	274	83	1	74	347	67	339	3.34e-135	402
Msa1410400	MtrunA17_Chr1g0176231	47.059	85	36	3	383	467	488	563	7.58e-13	70.9
Msa1410400	MtrunA17_Chr1g0178991	47.964	442	173	8	64	451	46	484	4.04e-125	373

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1410400	AT4G33050.3	69.586	411	112	3	59	469	87	484	0.0	579
Msa1410400	AT4G33050.5	69.586	411	112	3	59	469	159	556	0.0	579
Msa1410400	AT4G33050.2	63.697	449	112	4	59	469	87	522	0.0	561
Msa1410400	AT5G57010.1	60.573	454	143	7	25	470	70	495	0.0	545
Msa1410400	AT2G26190.3	68.889	405	120	5	68	469	126	527	0.0	543
Msa1410400	AT2G26190.1	68.889	405	120	5	68	469	126	527	0.0	543
Msa1410400	AT2G26190.2	79.853	273	54	1	68	340	126	397	2.71e-159	458
Msa1410400	AT3G13600.1	68.750	288	84	3	69	351	96	382	9.26e-143	422
Msa1410400	AT3G13600.1	51.807	83	35	2	386	467	520	598	1.16e-15	80.1
Msa1410400	AT3G13600.2	68.750	288	84	3	69	351	96	382	9.26e-143	422

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Find 126 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAATTTATAAGCTTTCT+TGG	0.184873	tig0023654:+45478	Msa1410400:CDS
AAAGGAAGCTTTGGAGGGAT+TGG	0.387195	tig0023654:+46046	Msa1410400:exon
AACAAAGGTTCCTACTCCTA+AGG	0.545400	tig0023654:+45711	Msa1410400:CDS
AACAAATTAATGCAATTATT+TGG	0.224184	tig0023654:+43670	Msa1410400:five_prime_UTR
AACAAGTTCATTTGTCTCCA+AGG	0.506581	tig0023654:+45893	Msa1410400:CDS
AAGAAAGCTTGAATGTTGAA+AGG	0.583819	tig0023654:-45095	None:intergenic
AAGTAATTGTTGAAAAGGGA+AGG	0.544030	tig0023654:+44963	Msa1410400:CDS
AAGTTAAAGGAAGCTTTGGA+GGG	0.593505	tig0023654:+46041	Msa1410400:exon
AATCCCATGTACACAAGCCT+AGG	0.574805	tig0023654:-45988	None:intergenic
AATGATTTACCGTTGTTGTA+TGG	0.471270	tig0023654:-43510	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTCATACACCATACAACAA+CGG	+	tig0023654:43501-43520	Msa1410400:five_prime_UTR	30.0%
AATGATTTACCGTTGTTGTA+TGG	-	tig0023654:43513-43532	None:intergenic	30.0%
CAGTTATTGGAGGTTAATTA+TGG	+	tig0023654:43604-43623	Msa1410400:five_prime_UTR	30.0%
TATTCAACTACTATATGGCA+TGG	+	tig0023654:43716-43735	Msa1410400:five_prime_UTR	30.0%
AGGTTTATCTAAGGATGAAA+AGG	+	tig0023654:44372-44391	Msa1410400:CDS	30.0%
AGAGTTTACCAGTAGAAAAA+TGG	-	tig0023654:44625-44644	None:intergenic	30.0%
ACAGATGAAGATTGTAAAAC+AGG	+	tig0023654:44682-44701	Msa1410400:intron	30.0%
CGCCAATGTATTAAATATCT+TGG	+	tig0023654:44823-44842	Msa1410400:CDS	30.0%
AGATCATGTAGATAACTTAC+TGG	-	tig0023654:44850-44869	None:intergenic	30.0%
AAGTAATTGTTGAAAAGGGA+AGG	+	tig0023654:44963-44982	Msa1410400:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0023654	gene	43410	46534	43410	ID=Msa1410400;Name=Msa1410400
tig0023654	mRNA	43410	46534	43410	ID=Msa1410400-mRNA-1;Parent=Msa1410400;Name=Msa1410400-mRNA-1;_AED=0.08;_eAED=0.08;_QI=326\|1\|1\|1\|0.85\|0.75\|8\|487\|476
tig0023654	exon	43410	43522	43410	ID=Msa1410400-mRNA-1:exon:26514;Parent=Msa1410400-mRNA-1
tig0023654	exon	43578	44231	43578	ID=Msa1410400-mRNA-1:exon:26515;Parent=Msa1410400-mRNA-1
tig0023654	exon	44364	44429	44364	ID=Msa1410400-mRNA-1:exon:26516;Parent=Msa1410400-mRNA-1
tig0023654	exon	44547	44635	44547	ID=Msa1410400-mRNA-1:exon:26517;Parent=Msa1410400-mRNA-1
tig0023654	exon	44759	44849	44759	ID=Msa1410400-mRNA-1:exon:26518;Parent=Msa1410400-mRNA-1
tig0023654	exon	44944	45174	44944	ID=Msa1410400-mRNA-1:exon:26519;Parent=Msa1410400-mRNA-1
tig0023654	exon	45430	45531	45430	ID=Msa1410400-mRNA-1:exon:26520;Parent=Msa1410400-mRNA-1
tig0023654	exon	45637	46534	45637	ID=Msa1410400-mRNA-1:exon:26521;Parent=Msa1410400-mRNA-1
tig0023654	five_prime_UTR	43410	43522	43410	ID=Msa1410400-mRNA-1:five_prime_utr;Parent=Msa1410400-mRNA-1
tig0023654	five_prime_UTR	43578	43790	43578	ID=Msa1410400-mRNA-1:five_prime_utr;Parent=Msa1410400-mRNA-1
tig0023654	CDS	43791	44231	43791	ID=Msa1410400-mRNA-1:cds;Parent=Msa1410400-mRNA-1
tig0023654	CDS	44364	44429	44364	ID=Msa1410400-mRNA-1:cds;Parent=Msa1410400-mRNA-1
tig0023654	CDS	44547	44635	44547	ID=Msa1410400-mRNA-1:cds;Parent=Msa1410400-mRNA-1
tig0023654	CDS	44759	44849	44759	ID=Msa1410400-mRNA-1:cds;Parent=Msa1410400-mRNA-1
tig0023654	CDS	44944	45174	44944	ID=Msa1410400-mRNA-1:cds;Parent=Msa1410400-mRNA-1
tig0023654	CDS	45430	45531	45430	ID=Msa1410400-mRNA-1:cds;Parent=Msa1410400-mRNA-1
tig0023654	CDS	45637	46047	45637	ID=Msa1410400-mRNA-1:cds;Parent=Msa1410400-mRNA-1
tig0023654	three_prime_UTR	46048	46534	46048	ID=Msa1410400-mRNA-1:three_prime_utr;Parent=Msa1410400-mRNA-1

Gene Sequence

>Msa1410400

ATGGGGGTGTCTGTTACTGATTTGTCAAATGGGTCCAATAAGATGAGTACTTCAAGCAGGTTGAGTGATTGCAGACCTCAGCATATGATTCTTGAAAGAAACCATTCATTTGTTCAAGATAAAGAACAAAACATGGATTCAACCAAAAGTGATCAATTCAAACATAAACCAGTTCCAGTGATTTCTCTGCCTGAAGCAGTTGTTTTTTCTTCTCCAAGACCAGTTTCTGAGCTTGATGCTGCTGCAACTACACTTCAAAAAGTATACAAGAGTTACCGAACTAGAAGAAACCTTGCAGATTGTGCAGTTGTAGTGGAAGAGTTATGGTGGAAAGCATTGGATTTTGCTGCTCTTAGAAGAAGTTCTGTTTCATTCTTTGATGTTGAGAAACCTGAAACTGCTGTGTCACGTTGGGCTAGAGCAAGAACAAGAGCAGCTAAGGTTGGAAAAGGTTTATCTAAGGATGAAAAGGCACAAAAATTAGCACTTCAACATTGGCTTGAAGCTATTGACCCGCGTCATCGATATGGACACAATCTTCACATGTATTATGATATCTGGTTTAAAAGCTTAAGTTCACAACCATTTTTCTACTGGTTGGATGTTGGAGATGGAAAAGAAATAAATCTTGAGAAATGTCCAAGAAGCACTGTTCTACAACGCCAATGTATTAAATATCTTGGACCAAAAGAAAGAGAAGAATATGAAGTAATTGTTGAAAAGGGAAGGTTGGTGTATAAACAAGACGGGAGGCTTGTGAGTACTGATGAGAAATCAAAATGGATATTTGTTCTCAGCACTGCAAGAGCCTTGTATGTGGGGAAAAAGCAAAAGGGTGCCTTTCAACATTCAAGCTTTCTTGCTGGTGGTGCAACCACAGCAGCTGGTAGATTAGTAGCAAATCAAGGTGTTCTTGAGGCTATTTGGCCATACAGTGGACACTACCATCCAACAGAAGAGAACTTCAAAGAATTTATAAGCTTTCTTGGAGAGCACGATGTAGACCTCACAAATGTAAAGAGATGTGCCATAGATGATGATACTTATTCAATTGTTGGAAATGATTCATTTGATGTCATCAATGAACCACAACAAACAAAGGTTCCTACTCCTAAGGTCAATGTCAATGATAACAATGTGACATCAACTCATAAAAAGGATGCTGTTGCAGCATTTAACGCCTCAAGGAGGTTGTCTTGCAAGTGGTCAACTGGAGCTGGTCCACGTATTGGTTGTGTTCGTGACTATCCTGAACACTTGCAATCAAAAGCATTGGAACAAGTTCATTTGTCTCCAAGGCCAAGTTTTTCAAGACTCAACAAATTTGGTCCAATTCCTTCTCCTAGGCCTAGCCCAAAAGTTAGAATGTCACCTAGGCTTGTGTACATGGGATTGCCTAGTCCAAGAAACCCAATTTCAACTGCAAGTTAA

Protein sequence

>Msa1410400

MGVSVTDLSNGSNKMSTSSRLSDCRPQHMILERNHSFVQDKEQNMDSTKSDQFKHKPVPVISLPEAVVFSSPRPVSELDAAATTLQKVYKSYRTRRNLADCAVVVEELWWKALDFAALRRSSVSFFDVEKPETAVSRWARARTRAAKVGKGLSKDEKAQKLALQHWLEAIDPRHRYGHNLHMYYDIWFKSLSSQPFFYWLDVGDGKEINLEKCPRSTVLQRQCIKYLGPKEREEYEVIVEKGRLVYKQDGRLVSTDEKSKWIFVLSTARALYVGKKQKGAFQHSSFLAGGATTAAGRLVANQGVLEAIWPYSGHYHPTEENFKEFISFLGEHDVDLTNVKRCAIDDDTYSIVGNDSFDVINEPQQTKVPTPKVNVNDNNVTSTHKKDAVAAFNASRRLSCKWSTGAGPRIGCVRDYPEHLQSKALEQVHLSPRPSFSRLNKFGPIPSPRPSPKVRMSPRLVYMGLPSPRNPISTAS*