AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1418720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1418720 MtrunA17_Chr8g0392151 97.510 241 6 0 1 241 1 241 5.25e-179 491
Msa1418720 MtrunA17_Chr1g0161601 48.889 90 45 1 14 103 49 137 4.23e-25 96.7
Msa1418720 MtrunA17_Chr4g0065041 33.793 145 74 3 15 158 10 133 4.99e-21 88.6
Msa1418720 MtrunA17_Chr2g0297251 35.172 145 72 4 15 158 39 162 2.36e-20 87.0
Msa1418720 MtrunA17_Chr7g0230511 30.345 145 78 4 15 158 11 133 4.19e-20 85.9
Msa1418720 MtrunA17_Chr1g0179781 35.374 147 71 6 15 158 9 134 5.20e-19 82.0
Msa1418720 MtrunA17_Chr5g0411151 35.616 146 71 5 15 158 37 161 2.00e-17 78.6
Msa1418720 MtrunA17_Chr7g0265291 33.974 156 77 6 15 165 9 143 5.59e-17 76.6
Msa1418720 MtrunA17_Chr4g0040071 34.932 146 72 5 15 158 17 141 6.50e-17 76.6
Msa1418720 MtrunA17_Chr8g0366581 34.932 146 72 5 15 158 35 159 3.74e-16 75.1
Msa1418720 MtrunA17_Chr5g0395181 32.877 146 75 5 15 158 23 147 9.13e-16 73.6
Msa1418720 MtrunA17_Chr2g0320551 31.788 151 86 5 13 159 4 141 4.90e-15 70.5
Msa1418720 MtrunA17_Chr2g0320561 29.936 157 93 5 13 165 4 147 2.71e-14 70.1
Msa1418720 MtrunA17_Chr2g0308401 29.936 157 92 6 13 164 4 147 3.53e-13 65.5
Msa1418720 MtrunA17_Chr4g0020811 29.936 157 92 6 13 164 6 149 3.57e-13 65.5
Msa1418720 MtrunA17_Chr2g0320521 34.021 97 60 3 13 105 6 102 1.52e-11 62.4
Msa1418720 MtrunA17_Chr8g0386511 31.250 112 54 3 50 158 4 95 1.95e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1418720 AT2G01818.1 43.421 152 66 4 15 161 13 149 9.34e-36 127
Msa1418720 AT3G60670.1 33.333 147 76 3 15 160 9 134 4.33e-22 91.7
Msa1418720 AT2G12646.1 33.793 145 74 3 15 158 8 131 1.08e-20 88.2
Msa1418720 AT2G12646.2 33.793 145 74 3 15 158 8 131 1.08e-20 88.2
Msa1418720 AT2G12646.3 33.793 145 74 3 15 158 5 128 1.10e-20 88.2
Msa1418720 AT1G21000.1 36.735 147 70 5 15 159 18 143 1.75e-18 82.0
Msa1418720 AT1G21000.2 36.735 147 70 5 15 159 15 140 1.77e-18 82.0
Msa1418720 AT1G43000.2 31.760 233 122 11 15 237 18 223 5.59e-18 80.1
Msa1418720 AT1G43000.1 31.760 233 122 11 15 237 11 216 6.77e-18 79.7
Msa1418720 AT1G76590.1 38.060 134 61 4 27 159 33 145 2.41e-17 79.0
Msa1418720 AT4G17900.1 31.875 160 87 4 2 159 23 162 4.28e-17 77.8
Msa1418720 AT1G31040.1 30.675 163 90 3 15 176 21 161 6.88e-17 77.8
Msa1418720 AT4G17900.2 33.333 147 76 4 15 159 22 148 8.00e-17 77.0
Msa1418720 AT2G27930.3 33.333 150 85 5 15 161 6 143 5.72e-16 73.6
Msa1418720 AT2G27930.2 33.333 150 85 5 15 161 6 143 5.72e-16 73.6
Msa1418720 AT2G27930.1 33.333 147 83 5 15 158 6 140 2.07e-15 72.4
Msa1418720 AT2G27930.4 33.333 147 83 5 15 158 6 140 2.07e-15 72.4
Msa1418720 AT5G46710.2 31.902 163 84 5 1 161 21 158 2.39e-15 71.6
Msa1418720 AT2G01818.2 39.175 97 44 2 65 161 4 85 3.63e-15 71.2
Msa1418720 AT5G46710.1 31.677 161 83 5 1 159 21 156 8.37e-15 71.6
Msa1418720 AT1G32700.1 31.447 159 86 5 2 158 1 138 1.23e-14 70.9
Msa1418720 AT5G46710.3 31.447 159 82 5 3 159 7 140 2.12e-14 70.1
Msa1418720 AT1G32700.2 35.294 119 55 4 41 158 2 99 7.83e-12 62.4

Find 72 sgRNAs with CRISPR-Local

Find 258 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTGATAAGTTGCTTTCTT+TGG 0.204312 tig0024639:-45669 None:intergenic
CAGGGTATCTTGAACAATTA+AGG 0.231703 tig0024639:+46334 Msa1418720:three_prime_UTR
TTTATACTTGAAACCCAATA+TGG 0.234084 tig0024639:-46378 None:intergenic
ATGATTCCGGTTCCGAATTA+AGG 0.235495 tig0024639:-45991 None:intergenic
CTTTGACTGCTCAAATATTC+AGG 0.261064 tig0024639:+45162 Msa1418720:CDS
AATTCCATTATACCCCATAT+TGG 0.274998 tig0024639:+46364 Msa1418720:three_prime_UTR
ACTATTCGTATCAAAGTCTT+AGG 0.326345 tig0024639:-46457 None:intergenic
AAACCCAATATGGGGTATAA+TGG 0.334826 tig0024639:-46368 None:intergenic
GTCCAAATCATCACTCAAAA+AGG 0.343718 tig0024639:+45885 Msa1418720:CDS
AAGCTCATTCTATTCATAGA+AGG 0.351713 tig0024639:+45080 Msa1418720:CDS
ATTCCATTATACCCCATATT+GGG 0.354865 tig0024639:+46365 Msa1418720:three_prime_UTR
GATTTACAGATATTCCTATC+AGG 0.363443 tig0024639:+45108 Msa1418720:CDS
CAATCTATATTCCTTTCCTT+TGG 0.367572 tig0024639:-44938 None:intergenic
TGATTTGGACCTTGGCTTTG+AGG 0.368181 tig0024639:-45872 None:intergenic
AATCAAAGGAAAAGAAATAA+AGG 0.369890 tig0024639:-46275 None:intergenic
TTAATTTCATCACCATCGTT+TGG 0.373365 tig0024639:+46513 Msa1418720:three_prime_UTR
ATAACTTCTAATAGGATGTT+TGG 0.374838 tig0024639:-46230 None:intergenic
TAAGAGAGAGAATGTGAAAA+GGG 0.380209 tig0024639:-44355 None:intergenic
TTGAGTGATGATTTGGACCT+TGG 0.380468 tig0024639:-45880 None:intergenic
TATGACTGATAACCTTAATT+CGG 0.380930 tig0024639:+45979 Msa1418720:CDS
TATTTGTGATAACTTCTAAT+AGG 0.390180 tig0024639:-46238 None:intergenic
GCTTCAGAAATAGATGATTC+CGG 0.410307 tig0024639:-46004 None:intergenic
TTCGTTTCTTAGCATATGTT+CGG 0.421437 tig0024639:+46106 Msa1418720:exon
CAAAGGAAAGGAATATAGAT+TGG 0.423544 tig0024639:+44939 Msa1418720:CDS
TTATACTTGAAACCCAATAT+GGG 0.425759 tig0024639:-46377 None:intergenic
GTAAGAGAGAGAATGTGAAA+AGG 0.436541 tig0024639:-44356 None:intergenic
CTTACATATCGAATAAAGAT+AGG 0.448689 tig0024639:+45576 Msa1418720:CDS
TGATAACCTTAATTCGGAAC+CGG 0.455008 tig0024639:+45985 Msa1418720:CDS
CATAGTAAAGTCAATAGCTT+CGG 0.480348 tig0024639:-45960 None:intergenic
GACTTATAGATCGGAGGCCG+AGG 0.481284 tig0024639:-45614 None:intergenic
AGGGTATCTTGAACAATTAA+GGG 0.484551 tig0024639:+46335 Msa1418720:three_prime_UTR
ATGGCGGAAAACATCCTGAT+AGG 0.486299 tig0024639:-45122 None:intergenic
TGCTGCCATCCCATGGTCAA+TGG 0.493459 tig0024639:+46579 Msa1418720:three_prime_UTR
GCTCTTATGAATAGTAGCTC+TGG 0.499499 tig0024639:+44968 Msa1418720:CDS
CCGTGCGGCAATTTGAAGCT+AGG 0.504383 tig0024639:+46157 Msa1418720:three_prime_UTR
ATTATCCTAAAATGATAACT+TGG 0.504942 tig0024639:-46307 None:intergenic
TTATCAGCTAGATTTAAGTC+AGG 0.519647 tig0024639:+45683 Msa1418720:CDS
CCGATCTATAAGTCTAAAGT+TGG 0.532512 tig0024639:+45623 Msa1418720:CDS
TGAAGCTGAGCCATGTGGGC+GGG 0.537407 tig0024639:+46021 Msa1418720:CDS
CGTTGTGAGTAGGAAAATCA+TGG 0.557197 tig0024639:-46202 None:intergenic
AGTGCTTCTGCAGCTCAGAA+TGG 0.561001 tig0024639:-45141 None:intergenic
CCAACTTTAGACTTATAGAT+CGG 0.562220 tig0024639:-45623 None:intergenic
TTTGGTAGCTCGTTGTGAGT+AGG 0.568439 tig0024639:-46212 None:intergenic
GGCAGTACATGTGAAGAATG+TGG 0.570625 tig0024639:+45707 Msa1418720:CDS
TAAGAAACGAAAACAAAGAC+AGG 0.585409 tig0024639:-46094 None:intergenic
GCAGTACATGTGAAGAATGT+GGG 0.588854 tig0024639:+45708 Msa1418720:CDS
CTGAAGCTGAGCCATGTGGG+CGG 0.592373 tig0024639:+46020 Msa1418720:CDS
ACTCTAATGCTGCCATCCCA+TGG 0.592551 tig0024639:+46572 Msa1418720:three_prime_UTR
TCAGCTAGATTTAAGTCAGG+AGG 0.597898 tig0024639:+45686 Msa1418720:CDS
CAACTGTACGTCAAACTCCT+CGG 0.598178 tig0024639:-46401 None:intergenic
AGGATCAATCACCAAAGGAA+AGG 0.601046 tig0024639:+44927 Msa1418720:CDS
CTGGTGCTCAATTATATGCA+AGG 0.606595 tig0024639:+45757 Msa1418720:CDS
GTTGACTCTTTCAACCAAAG+TGG 0.608258 tig0024639:+45914 Msa1418720:CDS
GGATTGTGCATCTCAAACCT+CGG 0.608993 tig0024639:+45597 Msa1418720:CDS
TACAAGATGAGCATAGTCGC+TGG 0.613510 tig0024639:+45738 Msa1418720:CDS
ATAGGGGAGCCTCAAAGCCA+AGG 0.624380 tig0024639:+45863 Msa1418720:CDS
TTTCTGAAGCTGAGCCATGT+GGG 0.625236 tig0024639:+46017 Msa1418720:CDS
TATACTTGAAACCCAATATG+GGG 0.636266 tig0024639:-46376 None:intergenic
ACAACTTCAACCCGCCCACA+TGG 0.636500 tig0024639:-46031 None:intergenic
AAAGACAGGCCTCTGAGGAG+TGG 0.638363 tig0024639:-46080 None:intergenic
GTGAGATGAATTCCAAACGA+TGG 0.640882 tig0024639:-46525 None:intergenic
GCTTCTGCAGCTCAGAATGG+CGG 0.645960 tig0024639:-45138 None:intergenic
TGATGATGCATCTACCACTT+TGG 0.646189 tig0024639:-45928 None:intergenic
GGGGAAGGATCAATCACCAA+AGG 0.648113 tig0024639:+44922 Msa1418720:CDS
ATTGTGCTGTTAGATTCTGT+AGG 0.654870 tig0024639:+45047 Msa1418720:CDS
ATTTCTGAAGCTGAGCCATG+TGG 0.657682 tig0024639:+46016 Msa1418720:CDS
ACTTTAGACTTATAGATCGG+AGG 0.659735 tig0024639:-45620 None:intergenic
AAAACAAAGACAGGCCTCTG+AGG 0.661748 tig0024639:-46085 None:intergenic
CCTAGCTTCAAATTGCCGCA+CGG 0.665330 tig0024639:-46157 None:intergenic
ACACAAGCAAGTAGAAAATG+CGG 0.685653 tig0024639:-44399 None:intergenic
TGGGTTTCAAGTATAAACCG+AGG 0.708166 tig0024639:+46384 Msa1418720:three_prime_UTR
TGTGAGTAGGAAAATCATGG+TGG 0.769697 tig0024639:-46199 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAAATATGAATCAAA+TGG - tig0024639:44634-44653 None:intergenic 10.0%
!!! TTTATTTTTGTTTTAAATGT+TGG + tig0024639:44646-44665 Msa1418720:intron 10.0%
!! AAAATAAAATATGAATCAAA+TGG - tig0024639:44634-44653 None:intergenic 10.0%
!!! TTTATTTTTGTTTTAAATGT+TGG + tig0024639:44646-44665 Msa1418720:intron 10.0%
!! TTATTCAATGTAATTTCAAT+AGG - tig0024639:45363-45382 None:intergenic 15.0%
!! AATAAACACAATTTATTTCA+TGG - tig0024639:45429-45448 None:intergenic 15.0%
!! TTATTCAATGTAATTTCAAT+AGG - tig0024639:45363-45382 None:intergenic 15.0%
!! AATAAACACAATTTATTTCA+TGG - tig0024639:45429-45448 None:intergenic 15.0%
!!! TATTTTGAATATCTGAGATT+AGG + tig0024639:44748-44767 Msa1418720:intron 20.0%
!!! TGTTTTGATTTGTTTTAATC+TGG + tig0024639:45288-45307 Msa1418720:intron 20.0%
!!! ATTGAATAAATTTTCCATGT+GGG + tig0024639:45374-45393 Msa1418720:intron 20.0%
!! AGTTTATGATCAATAACAAT+TGG - tig0024639:45501-45520 None:intergenic 20.0%
!! TATTTGTGATAACTTCTAAT+AGG - tig0024639:46241-46260 None:intergenic 20.0%
!! AATCAAAGGAAAAGAAATAA+AGG - tig0024639:46278-46297 None:intergenic 20.0%
!! ATTATCCTAAAATGATAACT+TGG - tig0024639:46310-46329 None:intergenic 20.0%
!!! TATTTTGAATATCTGAGATT+AGG + tig0024639:44748-44767 Msa1418720:intron 20.0%
!!! TGTTTTGATTTGTTTTAATC+TGG + tig0024639:45288-45307 Msa1418720:intron 20.0%
!!! ATTGAATAAATTTTCCATGT+GGG + tig0024639:45374-45393 Msa1418720:intron 20.0%
!! AGTTTATGATCAATAACAAT+TGG - tig0024639:45501-45520 None:intergenic 20.0%
!! TATTTGTGATAACTTCTAAT+AGG - tig0024639:46241-46260 None:intergenic 20.0%
!! AATCAAAGGAAAAGAAATAA+AGG - tig0024639:46278-46297 None:intergenic 20.0%
!! ATTATCCTAAAATGATAACT+TGG - tig0024639:46310-46329 None:intergenic 20.0%
!! AATTGTTCTTACCATGTTTT+TGG - tig0024639:44438-44457 None:intergenic 25.0%
! CAAAACTTTATAAGAATCCA+TGG - tig0024639:44480-44499 None:intergenic 25.0%
!! CTGATTCTGAATTAAGTTTT+TGG + tig0024639:44846-44865 Msa1418720:intron 25.0%
!!! TTCTGAATTAAGTTTTTGGT+TGG + tig0024639:44850-44869 Msa1418720:intron 25.0%
!!! TCTGAATTAAGTTTTTGGTT+GGG + tig0024639:44851-44870 Msa1418720:intron 25.0%
!!! TTGATTTGTTTTAATCTGGT+TGG + tig0024639:45292-45311 Msa1418720:intron 25.0%
!! CATTGAATAAATTTTCCATG+TGG + tig0024639:45373-45392 Msa1418720:intron 25.0%
! AATTTATAATTCAACCCACA+TGG - tig0024639:45391-45410 None:intergenic 25.0%
! CTTACATATCGAATAAAGAT+AGG + tig0024639:45576-45595 Msa1418720:CDS 25.0%
!!! TTTCTTTGGATTTTGATTCA+GGG - tig0024639:45658-45677 None:intergenic 25.0%
!! ACAAACATGCTCTTATTTAA+TGG + tig0024639:45782-45801 Msa1418720:intron 25.0%
! TATGACTGATAACCTTAATT+CGG + tig0024639:45979-45998 Msa1418720:CDS 25.0%
! ATAACTTCTAATAGGATGTT+TGG - tig0024639:46233-46252 None:intergenic 25.0%
! TTATACTTGAAACCCAATAT+GGG - tig0024639:46380-46399 None:intergenic 25.0%
! TTTATACTTGAAACCCAATA+TGG - tig0024639:46381-46400 None:intergenic 25.0%
!! AATTGTTCTTACCATGTTTT+TGG - tig0024639:44438-44457 None:intergenic 25.0%
! CAAAACTTTATAAGAATCCA+TGG - tig0024639:44480-44499 None:intergenic 25.0%
!! CTGATTCTGAATTAAGTTTT+TGG + tig0024639:44846-44865 Msa1418720:intron 25.0%
!!! TTCTGAATTAAGTTTTTGGT+TGG + tig0024639:44850-44869 Msa1418720:intron 25.0%
!!! TCTGAATTAAGTTTTTGGTT+GGG + tig0024639:44851-44870 Msa1418720:intron 25.0%
!!! TTGATTTGTTTTAATCTGGT+TGG + tig0024639:45292-45311 Msa1418720:intron 25.0%
!! CATTGAATAAATTTTCCATG+TGG + tig0024639:45373-45392 Msa1418720:intron 25.0%
! AATTTATAATTCAACCCACA+TGG - tig0024639:45391-45410 None:intergenic 25.0%
! CTTACATATCGAATAAAGAT+AGG + tig0024639:45576-45595 Msa1418720:CDS 25.0%
!!! TTTCTTTGGATTTTGATTCA+GGG - tig0024639:45658-45677 None:intergenic 25.0%
!! ACAAACATGCTCTTATTTAA+TGG + tig0024639:45782-45801 Msa1418720:intron 25.0%
! TATGACTGATAACCTTAATT+CGG + tig0024639:45979-45998 Msa1418720:CDS 25.0%
! ATAACTTCTAATAGGATGTT+TGG - tig0024639:46233-46252 None:intergenic 25.0%
! TTATACTTGAAACCCAATAT+GGG - tig0024639:46380-46399 None:intergenic 25.0%
! TTTATACTTGAAACCCAATA+TGG - tig0024639:46381-46400 None:intergenic 25.0%
TAAGAGAGAGAATGTGAAAA+GGG - tig0024639:44358-44377 None:intergenic 30.0%
!!! CATGTTTTTGGTTTGAGATT+GGG - tig0024639:44426-44445 None:intergenic 30.0%
!!! TCAATTTCCTGTTTTTTCCA+TGG + tig0024639:44460-44479 Msa1418720:intron 30.0%
TAAGAATCCATGGAAAAAAC+AGG - tig0024639:44470-44489 None:intergenic 30.0%
AACGAAATCAGAATCAGAAT+AGG - tig0024639:44511-44530 None:intergenic 30.0%
GAAATTTGCATGTGAAGATT+TGG + tig0024639:44710-44729 Msa1418720:intron 30.0%
! GAATATCTGAGATTAGGTTT+AGG + tig0024639:44754-44773 Msa1418720:intron 30.0%
!! GGTTCTTGAATTGATTTTGT+GGG + tig0024639:44872-44891 Msa1418720:intron 30.0%
!!! TGAATAATGATGGATTTTGC+AGG + tig0024639:44898-44917 Msa1418720:intron 30.0%
CAATCTATATTCCTTTCCTT+TGG - tig0024639:44941-44960 None:intergenic 30.0%
CAAAGGAAAGGAATATAGAT+TGG + tig0024639:44939-44958 Msa1418720:CDS 30.0%
AAGCTCATTCTATTCATAGA+AGG + tig0024639:45080-45099 Msa1418720:CDS 30.0%
GATTTACAGATATTCCTATC+AGG + tig0024639:45108-45127 Msa1418720:CDS 30.0%
GATAGACTTTAATGATCTGA+TGG + tig0024639:45316-45335 Msa1418720:intron 30.0%
TCAATAGGTTCAACCATTAA+TGG - tig0024639:45348-45367 None:intergenic 30.0%
AACAAACAATGACAGTACAA+AGG - tig0024639:45455-45474 None:intergenic 30.0%
CCAACTTTAGACTTATAGAT+CGG - tig0024639:45626-45645 None:intergenic 30.0%
!!! CTTTCTTTGGATTTTGATTC+AGG - tig0024639:45659-45678 None:intergenic 30.0%
TTATCAGCTAGATTTAAGTC+AGG + tig0024639:45683-45702 Msa1418720:CDS 30.0%
CATAGTAAAGTCAATAGCTT+CGG - tig0024639:45963-45982 None:intergenic 30.0%
TAAGAAACGAAAACAAAGAC+AGG - tig0024639:46097-46116 None:intergenic 30.0%
TTCGTTTCTTAGCATATGTT+CGG + tig0024639:46106-46125 Msa1418720:exon 30.0%
CTTGGAAGCAAAAAAATCAA+AGG - tig0024639:46292-46311 None:intergenic 30.0%
! TGCTTCCAAGTTATCATTTT+AGG + tig0024639:46302-46321 Msa1418720:three_prime_UTR 30.0%
!!! TCATTTTAGGATAATGAGAC+AGG + tig0024639:46315-46334 Msa1418720:three_prime_UTR 30.0%
!!! CATTTTAGGATAATGAGACA+GGG + tig0024639:46316-46335 Msa1418720:three_prime_UTR 30.0%
! AGGGTATCTTGAACAATTAA+GGG + tig0024639:46335-46354 Msa1418720:three_prime_UTR 30.0%
AATTCCATTATACCCCATAT+TGG + tig0024639:46364-46383 Msa1418720:three_prime_UTR 30.0%
ATTCCATTATACCCCATATT+GGG + tig0024639:46365-46384 Msa1418720:three_prime_UTR 30.0%
TATACTTGAAACCCAATATG+GGG - tig0024639:46379-46398 None:intergenic 30.0%
ACTATTCGTATCAAAGTCTT+AGG - tig0024639:46460-46479 None:intergenic 30.0%
TTAATTTCATCACCATCGTT+TGG + tig0024639:46513-46532 Msa1418720:three_prime_UTR 30.0%
TAAGAGAGAGAATGTGAAAA+GGG - tig0024639:44358-44377 None:intergenic 30.0%
!!! CATGTTTTTGGTTTGAGATT+GGG - tig0024639:44426-44445 None:intergenic 30.0%
!!! TCAATTTCCTGTTTTTTCCA+TGG + tig0024639:44460-44479 Msa1418720:intron 30.0%
TAAGAATCCATGGAAAAAAC+AGG - tig0024639:44470-44489 None:intergenic 30.0%
AACGAAATCAGAATCAGAAT+AGG - tig0024639:44511-44530 None:intergenic 30.0%
GAAATTTGCATGTGAAGATT+TGG + tig0024639:44710-44729 Msa1418720:intron 30.0%
! GAATATCTGAGATTAGGTTT+AGG + tig0024639:44754-44773 Msa1418720:intron 30.0%
!! GGTTCTTGAATTGATTTTGT+GGG + tig0024639:44872-44891 Msa1418720:intron 30.0%
!!! TGAATAATGATGGATTTTGC+AGG + tig0024639:44898-44917 Msa1418720:intron 30.0%
CAATCTATATTCCTTTCCTT+TGG - tig0024639:44941-44960 None:intergenic 30.0%
CAAAGGAAAGGAATATAGAT+TGG + tig0024639:44939-44958 Msa1418720:CDS 30.0%
AAGCTCATTCTATTCATAGA+AGG + tig0024639:45080-45099 Msa1418720:CDS 30.0%
GATTTACAGATATTCCTATC+AGG + tig0024639:45108-45127 Msa1418720:CDS 30.0%
GATAGACTTTAATGATCTGA+TGG + tig0024639:45316-45335 Msa1418720:intron 30.0%
TCAATAGGTTCAACCATTAA+TGG - tig0024639:45348-45367 None:intergenic 30.0%
AACAAACAATGACAGTACAA+AGG - tig0024639:45455-45474 None:intergenic 30.0%
CCAACTTTAGACTTATAGAT+CGG - tig0024639:45626-45645 None:intergenic 30.0%
!!! CTTTCTTTGGATTTTGATTC+AGG - tig0024639:45659-45678 None:intergenic 30.0%
TTATCAGCTAGATTTAAGTC+AGG + tig0024639:45683-45702 Msa1418720:CDS 30.0%
CATAGTAAAGTCAATAGCTT+CGG - tig0024639:45963-45982 None:intergenic 30.0%
TAAGAAACGAAAACAAAGAC+AGG - tig0024639:46097-46116 None:intergenic 30.0%
TTCGTTTCTTAGCATATGTT+CGG + tig0024639:46106-46125 Msa1418720:exon 30.0%
CTTGGAAGCAAAAAAATCAA+AGG - tig0024639:46292-46311 None:intergenic 30.0%
! TGCTTCCAAGTTATCATTTT+AGG + tig0024639:46302-46321 Msa1418720:three_prime_UTR 30.0%
!!! TCATTTTAGGATAATGAGAC+AGG + tig0024639:46315-46334 Msa1418720:three_prime_UTR 30.0%
!!! CATTTTAGGATAATGAGACA+GGG + tig0024639:46316-46335 Msa1418720:three_prime_UTR 30.0%
! AGGGTATCTTGAACAATTAA+GGG + tig0024639:46335-46354 Msa1418720:three_prime_UTR 30.0%
AATTCCATTATACCCCATAT+TGG + tig0024639:46364-46383 Msa1418720:three_prime_UTR 30.0%
ATTCCATTATACCCCATATT+GGG + tig0024639:46365-46384 Msa1418720:three_prime_UTR 30.0%
TATACTTGAAACCCAATATG+GGG - tig0024639:46379-46398 None:intergenic 30.0%
ACTATTCGTATCAAAGTCTT+AGG - tig0024639:46460-46479 None:intergenic 30.0%
TTAATTTCATCACCATCGTT+TGG + tig0024639:46513-46532 Msa1418720:three_prime_UTR 30.0%
GTAAGAGAGAGAATGTGAAA+AGG - tig0024639:44359-44378 None:intergenic 35.0%
ACACAAGCAAGTAGAAAATG+CGG - tig0024639:44402-44421 None:intergenic 35.0%
!!! CCATGTTTTTGGTTTGAGAT+TGG - tig0024639:44427-44446 None:intergenic 35.0%
CCAATCTCAAACCAAAAACA+TGG + tig0024639:44424-44443 Msa1418720:exon 35.0%
!! GGGTTCTTGAATTGATTTTG+TGG + tig0024639:44871-44890 Msa1418720:intron 35.0%
!! TTGTGGGTACTGAATAATGA+TGG + tig0024639:44888-44907 Msa1418720:intron 35.0%
!!! ATAATGATGGATTTTGCAGG+TGG + tig0024639:44901-44920 Msa1418720:intron 35.0%
!!! TAATGATGGATTTTGCAGGT+GGG + tig0024639:44902-44921 Msa1418720:intron 35.0%
! ATTGTGCTGTTAGATTCTGT+AGG + tig0024639:45047-45066 Msa1418720:CDS 35.0%
CTTTGACTGCTCAAATATTC+AGG + tig0024639:45162-45181 Msa1418720:CDS 35.0%
! CTGATGGACATTACCATTAA+TGG + tig0024639:45332-45351 Msa1418720:intron 35.0%
CGTACAATTGTTCCATGATT+TGG + tig0024639:45549-45568 Msa1418720:intron 35.0%
TATGTAAGTCTGCCAAATCA+TGG - tig0024639:45564-45583 None:intergenic 35.0%
ACTTTAGACTTATAGATCGG+AGG - tig0024639:45623-45642 None:intergenic 35.0%
! CCGATCTATAAGTCTAAAGT+TGG + tig0024639:45623-45642 Msa1418720:CDS 35.0%
! AGCTGATAAGTTGCTTTCTT+TGG - tig0024639:45672-45691 None:intergenic 35.0%
! ATCATGTGCTGTTGTTAGAT+AGG + tig0024639:45845-45864 Msa1418720:intron 35.0%
! TCATGTGCTGTTGTTAGATA+GGG + tig0024639:45846-45865 Msa1418720:intron 35.0%
GTCCAAATCATCACTCAAAA+AGG + tig0024639:45885-45904 Msa1418720:CDS 35.0%
!!! CTCCTTTTTGAGTGATGATT+TGG - tig0024639:45890-45909 None:intergenic 35.0%
! AAGTCAATAGCTTCGGTTTT+CGG - tig0024639:45956-45975 None:intergenic 35.0%
TGATAACCTTAATTCGGAAC+CGG + tig0024639:45985-46004 Msa1418720:CDS 35.0%
GCTTCAGAAATAGATGATTC+CGG - tig0024639:46007-46026 None:intergenic 35.0%
! CAGGGTATCTTGAACAATTA+AGG + tig0024639:46334-46353 Msa1418720:three_prime_UTR 35.0%
AAACCCAATATGGGGTATAA+TGG - tig0024639:46371-46390 None:intergenic 35.0%
CATGAAAAACCATTGACCAT+GGG - tig0024639:46591-46610 None:intergenic 35.0%
ACATGAAAAACCATTGACCA+TGG - tig0024639:46592-46611 None:intergenic 35.0%
GTAAGAGAGAGAATGTGAAA+AGG - tig0024639:44359-44378 None:intergenic 35.0%
ACACAAGCAAGTAGAAAATG+CGG - tig0024639:44402-44421 None:intergenic 35.0%
!!! CCATGTTTTTGGTTTGAGAT+TGG - tig0024639:44427-44446 None:intergenic 35.0%
CCAATCTCAAACCAAAAACA+TGG + tig0024639:44424-44443 Msa1418720:exon 35.0%
!! GGGTTCTTGAATTGATTTTG+TGG + tig0024639:44871-44890 Msa1418720:intron 35.0%
!! TTGTGGGTACTGAATAATGA+TGG + tig0024639:44888-44907 Msa1418720:intron 35.0%
!!! ATAATGATGGATTTTGCAGG+TGG + tig0024639:44901-44920 Msa1418720:intron 35.0%
!!! TAATGATGGATTTTGCAGGT+GGG + tig0024639:44902-44921 Msa1418720:intron 35.0%
! ATTGTGCTGTTAGATTCTGT+AGG + tig0024639:45047-45066 Msa1418720:CDS 35.0%
CTTTGACTGCTCAAATATTC+AGG + tig0024639:45162-45181 Msa1418720:CDS 35.0%
! CTGATGGACATTACCATTAA+TGG + tig0024639:45332-45351 Msa1418720:intron 35.0%
CGTACAATTGTTCCATGATT+TGG + tig0024639:45549-45568 Msa1418720:intron 35.0%
TATGTAAGTCTGCCAAATCA+TGG - tig0024639:45564-45583 None:intergenic 35.0%
ACTTTAGACTTATAGATCGG+AGG - tig0024639:45623-45642 None:intergenic 35.0%
! CCGATCTATAAGTCTAAAGT+TGG + tig0024639:45623-45642 Msa1418720:CDS 35.0%
! AGCTGATAAGTTGCTTTCTT+TGG - tig0024639:45672-45691 None:intergenic 35.0%
! ATCATGTGCTGTTGTTAGAT+AGG + tig0024639:45845-45864 Msa1418720:intron 35.0%
! TCATGTGCTGTTGTTAGATA+GGG + tig0024639:45846-45865 Msa1418720:intron 35.0%
GTCCAAATCATCACTCAAAA+AGG + tig0024639:45885-45904 Msa1418720:CDS 35.0%
!!! CTCCTTTTTGAGTGATGATT+TGG - tig0024639:45890-45909 None:intergenic 35.0%
! AAGTCAATAGCTTCGGTTTT+CGG - tig0024639:45956-45975 None:intergenic 35.0%
TGATAACCTTAATTCGGAAC+CGG + tig0024639:45985-46004 Msa1418720:CDS 35.0%
GCTTCAGAAATAGATGATTC+CGG - tig0024639:46007-46026 None:intergenic 35.0%
! CAGGGTATCTTGAACAATTA+AGG + tig0024639:46334-46353 Msa1418720:three_prime_UTR 35.0%
AAACCCAATATGGGGTATAA+TGG - tig0024639:46371-46390 None:intergenic 35.0%
CATGAAAAACCATTGACCAT+GGG - tig0024639:46591-46610 None:intergenic 35.0%
ACATGAAAAACCATTGACCA+TGG - tig0024639:46592-46611 None:intergenic 35.0%
!!! AATGATGGATTTTGCAGGTG+GGG + tig0024639:44903-44922 Msa1418720:intron 40.0%
AGGATCAATCACCAAAGGAA+AGG + tig0024639:44927-44946 Msa1418720:CDS 40.0%
GCTCTTATGAATAGTAGCTC+TGG + tig0024639:44968-44987 Msa1418720:CDS 40.0%
TCAGCTAGATTTAAGTCAGG+AGG + tig0024639:45686-45705 Msa1418720:CDS 40.0%
GCAGTACATGTGAAGAATGT+GGG + tig0024639:45708-45727 Msa1418720:CDS 40.0%
CTGGTGCTCAATTATATGCA+AGG + tig0024639:45757-45776 Msa1418720:CDS 40.0%
! CATGTGCTGTTGTTAGATAG+GGG + tig0024639:45847-45866 Msa1418720:intron 40.0%
! TTGAGTGATGATTTGGACCT+TGG - tig0024639:45883-45902 None:intergenic 40.0%
! GTTGACTCTTTCAACCAAAG+TGG + tig0024639:45914-45933 Msa1418720:CDS 40.0%
! TGATGATGCATCTACCACTT+TGG - tig0024639:45931-45950 None:intergenic 40.0%
ATGATTCCGGTTCCGAATTA+AGG - tig0024639:45994-46013 None:intergenic 40.0%
TGTGAGTAGGAAAATCATGG+TGG - tig0024639:46202-46221 None:intergenic 40.0%
CGTTGTGAGTAGGAAAATCA+TGG - tig0024639:46205-46224 None:intergenic 40.0%
! TGGGTTTCAAGTATAAACCG+AGG + tig0024639:46384-46403 Msa1418720:three_prime_UTR 40.0%
GTGAGATGAATTCCAAACGA+TGG - tig0024639:46528-46547 None:intergenic 40.0%
AAAAACCATTGACCATGGGA+TGG - tig0024639:46587-46606 None:intergenic 40.0%
!!! AATGATGGATTTTGCAGGTG+GGG + tig0024639:44903-44922 Msa1418720:intron 40.0%
AGGATCAATCACCAAAGGAA+AGG + tig0024639:44927-44946 Msa1418720:CDS 40.0%
GCTCTTATGAATAGTAGCTC+TGG + tig0024639:44968-44987 Msa1418720:CDS 40.0%
TCAGCTAGATTTAAGTCAGG+AGG + tig0024639:45686-45705 Msa1418720:CDS 40.0%
GCAGTACATGTGAAGAATGT+GGG + tig0024639:45708-45727 Msa1418720:CDS 40.0%
CTGGTGCTCAATTATATGCA+AGG + tig0024639:45757-45776 Msa1418720:CDS 40.0%
! CATGTGCTGTTGTTAGATAG+GGG + tig0024639:45847-45866 Msa1418720:intron 40.0%
! TTGAGTGATGATTTGGACCT+TGG - tig0024639:45883-45902 None:intergenic 40.0%
! GTTGACTCTTTCAACCAAAG+TGG + tig0024639:45914-45933 Msa1418720:CDS 40.0%
! TGATGATGCATCTACCACTT+TGG - tig0024639:45931-45950 None:intergenic 40.0%
ATGATTCCGGTTCCGAATTA+AGG - tig0024639:45994-46013 None:intergenic 40.0%
TGTGAGTAGGAAAATCATGG+TGG - tig0024639:46202-46221 None:intergenic 40.0%
CGTTGTGAGTAGGAAAATCA+TGG - tig0024639:46205-46224 None:intergenic 40.0%
! TGGGTTTCAAGTATAAACCG+AGG + tig0024639:46384-46403 Msa1418720:three_prime_UTR 40.0%
GTGAGATGAATTCCAAACGA+TGG - tig0024639:46528-46547 None:intergenic 40.0%
AAAAACCATTGACCATGGGA+TGG - tig0024639:46587-46606 None:intergenic 40.0%
! ATGGCGGAAAACATCCTGAT+AGG - tig0024639:45125-45144 None:intergenic 45.0%
GGATTGTGCATCTCAAACCT+CGG + tig0024639:45597-45616 Msa1418720:CDS 45.0%
GGCAGTACATGTGAAGAATG+TGG + tig0024639:45707-45726 Msa1418720:CDS 45.0%
TACAAGATGAGCATAGTCGC+TGG + tig0024639:45738-45757 Msa1418720:CDS 45.0%
!! TGATTTGGACCTTGGCTTTG+AGG - tig0024639:45875-45894 None:intergenic 45.0%
!! ATTTCTGAAGCTGAGCCATG+TGG + tig0024639:46016-46035 Msa1418720:CDS 45.0%
!! TTTCTGAAGCTGAGCCATGT+GGG + tig0024639:46017-46036 Msa1418720:CDS 45.0%
! TCTGAGGAGTGGTTTTTCTC+GGG - tig0024639:46072-46091 None:intergenic 45.0%
!! CTCTGAGGAGTGGTTTTTCT+CGG - tig0024639:46073-46092 None:intergenic 45.0%
AAAACAAAGACAGGCCTCTG+AGG - tig0024639:46088-46107 None:intergenic 45.0%
! ATTTTCACAGCATCACCGTG+CGG + tig0024639:46142-46161 Msa1418720:three_prime_UTR 45.0%
! TTTGGTAGCTCGTTGTGAGT+AGG - tig0024639:46215-46234 None:intergenic 45.0%
CAACTGTACGTCAAACTCCT+CGG - tig0024639:46404-46423 None:intergenic 45.0%
! ATGGCGGAAAACATCCTGAT+AGG - tig0024639:45125-45144 None:intergenic 45.0%
GGATTGTGCATCTCAAACCT+CGG + tig0024639:45597-45616 Msa1418720:CDS 45.0%
GGCAGTACATGTGAAGAATG+TGG + tig0024639:45707-45726 Msa1418720:CDS 45.0%
TACAAGATGAGCATAGTCGC+TGG + tig0024639:45738-45757 Msa1418720:CDS 45.0%
!! TGATTTGGACCTTGGCTTTG+AGG - tig0024639:45875-45894 None:intergenic 45.0%
!! ATTTCTGAAGCTGAGCCATG+TGG + tig0024639:46016-46035 Msa1418720:CDS 45.0%
!! TTTCTGAAGCTGAGCCATGT+GGG + tig0024639:46017-46036 Msa1418720:CDS 45.0%
! TCTGAGGAGTGGTTTTTCTC+GGG - tig0024639:46072-46091 None:intergenic 45.0%
!! CTCTGAGGAGTGGTTTTTCT+CGG - tig0024639:46073-46092 None:intergenic 45.0%
AAAACAAAGACAGGCCTCTG+AGG - tig0024639:46088-46107 None:intergenic 45.0%
! ATTTTCACAGCATCACCGTG+CGG + tig0024639:46142-46161 Msa1418720:three_prime_UTR 45.0%
! TTTGGTAGCTCGTTGTGAGT+AGG - tig0024639:46215-46234 None:intergenic 45.0%
CAACTGTACGTCAAACTCCT+CGG - tig0024639:46404-46423 None:intergenic 45.0%
! ATGGATTTTGCAGGTGGGGA+AGG + tig0024639:44907-44926 Msa1418720:intron 50.0%
GGGGAAGGATCAATCACCAA+AGG + tig0024639:44922-44941 Msa1418720:CDS 50.0%
AGTGCTTCTGCAGCTCAGAA+TGG - tig0024639:45144-45163 None:intergenic 50.0%
CGAGAAAAACCACTCCTCAG+AGG + tig0024639:46071-46090 Msa1418720:CDS 50.0%
CCTAGCTTCAAATTGCCGCA+CGG - tig0024639:46160-46179 None:intergenic 50.0%
ACTCTAATGCTGCCATCCCA+TGG + tig0024639:46572-46591 Msa1418720:three_prime_UTR 50.0%
! ATGGATTTTGCAGGTGGGGA+AGG + tig0024639:44907-44926 Msa1418720:intron 50.0%
GGGGAAGGATCAATCACCAA+AGG + tig0024639:44922-44941 Msa1418720:CDS 50.0%
AGTGCTTCTGCAGCTCAGAA+TGG - tig0024639:45144-45163 None:intergenic 50.0%
CGAGAAAAACCACTCCTCAG+AGG + tig0024639:46071-46090 Msa1418720:CDS 50.0%
CCTAGCTTCAAATTGCCGCA+CGG - tig0024639:46160-46179 None:intergenic 50.0%
ACTCTAATGCTGCCATCCCA+TGG + tig0024639:46572-46591 Msa1418720:three_prime_UTR 50.0%
GCTTCTGCAGCTCAGAATGG+CGG - tig0024639:45141-45160 None:intergenic 55.0%
GACTTATAGATCGGAGGCCG+AGG - tig0024639:45617-45636 None:intergenic 55.0%
ATAGGGGAGCCTCAAAGCCA+AGG + tig0024639:45863-45882 Msa1418720:CDS 55.0%
ACAACTTCAACCCGCCCACA+TGG - tig0024639:46034-46053 None:intergenic 55.0%
! AAAGACAGGCCTCTGAGGAG+TGG - tig0024639:46083-46102 None:intergenic 55.0%
! CCGTGCGGCAATTTGAAGCT+AGG + tig0024639:46157-46176 Msa1418720:three_prime_UTR 55.0%
TGCTGCCATCCCATGGTCAA+TGG + tig0024639:46579-46598 Msa1418720:three_prime_UTR 55.0%
GCTTCTGCAGCTCAGAATGG+CGG - tig0024639:45141-45160 None:intergenic 55.0%
GACTTATAGATCGGAGGCCG+AGG - tig0024639:45617-45636 None:intergenic 55.0%
ATAGGGGAGCCTCAAAGCCA+AGG + tig0024639:45863-45882 Msa1418720:CDS 55.0%
ACAACTTCAACCCGCCCACA+TGG - tig0024639:46034-46053 None:intergenic 55.0%
! AAAGACAGGCCTCTGAGGAG+TGG - tig0024639:46083-46102 None:intergenic 55.0%
! CCGTGCGGCAATTTGAAGCT+AGG + tig0024639:46157-46176 Msa1418720:three_prime_UTR 55.0%
TGCTGCCATCCCATGGTCAA+TGG + tig0024639:46579-46598 Msa1418720:three_prime_UTR 55.0%
CTGAAGCTGAGCCATGTGGG+CGG + tig0024639:46020-46039 Msa1418720:CDS 60.0%
TGAAGCTGAGCCATGTGGGC+GGG + tig0024639:46021-46040 Msa1418720:CDS 60.0%
CTGAAGCTGAGCCATGTGGG+CGG + tig0024639:46020-46039 Msa1418720:CDS 60.0%
TGAAGCTGAGCCATGTGGGC+GGG + tig0024639:46021-46040 Msa1418720:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0024639 gene 44333 46635 44333 ID=Msa1418720;Name=Msa1418720
tig0024639 mRNA 44333 46635 44333 ID=Msa1418720-mRNA-1;Parent=Msa1418720;Name=Msa1418720-mRNA-1;_AED=0.07;_eAED=0.07;_QI=110|1|1|1|1|1|4|518|241
tig0024639 exon 44333 44445 44333 ID=Msa1418720-mRNA-1:exon:483;Parent=Msa1418720-mRNA-1
tig0024639 exon 44920 45183 44920 ID=Msa1418720-mRNA-1:exon:484;Parent=Msa1418720-mRNA-1
tig0024639 exon 45575 45778 45575 ID=Msa1418720-mRNA-1:exon:485;Parent=Msa1418720-mRNA-1
tig0024639 exon 45863 46635 45863 ID=Msa1418720-mRNA-1:exon:486;Parent=Msa1418720-mRNA-1
tig0024639 five_prime_UTR 44333 44442 44333 ID=Msa1418720-mRNA-1:five_prime_utr;Parent=Msa1418720-mRNA-1
tig0024639 CDS 44443 44445 44443 ID=Msa1418720-mRNA-1:cds;Parent=Msa1418720-mRNA-1
tig0024639 CDS 44920 45183 44920 ID=Msa1418720-mRNA-1:cds;Parent=Msa1418720-mRNA-1
tig0024639 CDS 45575 45778 45575 ID=Msa1418720-mRNA-1:cds;Parent=Msa1418720-mRNA-1
tig0024639 CDS 45863 46117 45863 ID=Msa1418720-mRNA-1:cds;Parent=Msa1418720-mRNA-1
tig0024639 three_prime_UTR 46118 46635 46118 ID=Msa1418720-mRNA-1:three_prime_utr;Parent=Msa1418720-mRNA-1
Gene Sequence

>Msa1418720

ATGGTGGGGAAGGATCAATCACCAAAGGAAAGGAATATAGATTGGATTAGAGCTCTTATGAATAGTAGCTCTGGCTATTGTGATGATCATCGTGATCTTCGATCTAATGAGAAGAATACTTTCTGTGTAGATTGTGCTGTTAGATTCTGTAGGCACTGTAAAGAAGCTCATTCTATTCATAGAAGGTTTCAGATTTACAGATATTCCTATCAGGATGTTTTCCGCCATTCTGAGCTGCAGAAGCACTTTGACTGCTCAAATATTCAGACTTACATATCGAATAAAGATAGGATTGTGCATCTCAAACCTCGGCCTCCGATCTATAAGTCTAAAGTTGGTGATCAATGCCCTGAATCAAAATCCAAAGAAAGCAACTTATCAGCTAGATTTAAGTCAGGAGGCAGTACATGTGAAGAATGTGGGAAACACTTACAAGATGAGCATAGTCGCTGGTGCTCAATTATATGCAAGATAGGGGAGCCTCAAAGCCAAGGTCCAAATCATCACTCAAAAAGGAGTTTAGTTGACTCTTTCAACCAAAGTGGTAGATGCATCATCACTCCGAAAACCGAAGCTATTGACTTTACTATGACTGATAACCTTAATTCGGAACCGGAATCATCTATTTCTGAAGCTGAGCCATGTGGGCGGGTTGAAGTTGTGAATTTCAGAAAGCGCCCGAGAAAAACCACTCCTCAGAGGCCTGTCTTTGTTTTCGTTTCTTAG
Protein sequence

>Msa1418720

MVGKDQSPKERNIDWIRALMNSSSGYCDDHRDLRSNEKNTFCVDCAVRFCRHCKEAHSIHRRFQIYRYSYQDVFRHSELQKHFDCSNIQTYISNKDRIVHLKPRPPIYKSKVGDQCPESKSKESNLSARFKSGGSTCEECGKHLQDEHSRWCSIICKIGEPQSQGPNHHSKRSLVDSFNQSGRCIITPKTEAIDFTMTDNLNSEPESSISEAEPCGRVEVVNFRKRPRKTTPQRPVFVFVS*