Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1421380 | A0A396HA82 | 75.294 | 85 | 15 | 1 | 3 | 87 | 64 | 142 | 1.82e-36 | 129 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0939650 | Msa1421380 | 0.882351 | 1.120569e-70 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1421380 | MtrunA17_Chr6g0455241 | 75.294 | 85 | 15 | 1 | 3 | 87 | 64 | 142 | 3.50e-40 | 129 |
| Msa1421380 | MtrunA17_Chr6g0455271 | 72.368 | 76 | 15 | 1 | 3 | 78 | 28 | 97 | 1.65e-31 | 107 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTGCTTGCCTTCTCAAAA+TGG | 0.248342 | tig0024950:-59940 | Msa1421380:CDS |
| TCATGCAGCAAGAATGCTTT+TGG | 0.265450 | tig0024950:-60043 | Msa1421380:CDS |
| TAAGTACCACACCTATTGTT+AGG | 0.386678 | tig0024950:+59910 | None:intergenic |
| GTACGATAGCCTTGAACTAT+AGG | 0.400696 | tig0024950:+59961 | None:intergenic |
| GTTAAGTGTCCTATAGTTCA+AGG | 0.442985 | tig0024950:-59970 | Msa1421380:CDS |
| CAAACACAAACTGGTAGAAC+TGG | 0.449515 | tig0024950:-60012 | Msa1421380:CDS |
| GATTTGAGTCAAACACAAAC+TGG | 0.464256 | tig0024950:-60021 | Msa1421380:CDS |
| CAAAGAAGAAAATGAACAAA+GGG | 0.478510 | tig0024950:-60146 | None:intergenic |
| ATGGAGGTGGACCTAACAAT+AGG | 0.486497 | tig0024950:-59921 | Msa1421380:CDS |
| GTAATTGGATTAATAAGATA+AGG | 0.501668 | tig0024950:+60108 | None:intergenic |
| TTGCCTTCTCAAAATGGAGG+TGG | 0.550109 | tig0024950:-59934 | Msa1421380:CDS |
| CCAAAGAAGAAAATGAACAA+AGG | 0.606112 | tig0024950:-60147 | None:intergenic |
| TGCTTGCCTTCTCAAAATGG+AGG | 0.620754 | tig0024950:-59937 | Msa1421380:CDS |
| GGTAGAACTGGTAACAACAA+TGG | 0.669601 | tig0024950:-60000 | Msa1421380:CDS |
| GGTGGACCTAACAATAGGTG+TGG | 0.694293 | tig0024950:-59916 | Msa1421380:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTAATTGGATTAATAAGATA+AGG | + | tig0024950:59924-59943 | None:intergenic | 20.0% |
| !! | ATGTTTTGCTTAGATGTAAT+TGG | + | tig0024950:59939-59958 | None:intergenic | 25.0% |
| GATTTGAGTCAAACACAAAC+TGG | - | tig0024950:60008-60027 | Msa1421380:CDS | 35.0% | |
| GTTAAGTGTCCTATAGTTCA+AGG | - | tig0024950:60059-60078 | Msa1421380:CDS | 35.0% | |
| TAAGTACCACACCTATTGTT+AGG | + | tig0024950:60122-60141 | None:intergenic | 35.0% | |
| ! | TCATGCAGCAAGAATGCTTT+TGG | - | tig0024950:59986-60005 | Msa1421380:CDS | 40.0% |
| CAAACACAAACTGGTAGAAC+TGG | - | tig0024950:60017-60036 | Msa1421380:CDS | 40.0% | |
| GGTAGAACTGGTAACAACAA+TGG | - | tig0024950:60029-60048 | Msa1421380:CDS | 40.0% | |
| ! | GTACGATAGCCTTGAACTAT+AGG | + | tig0024950:60071-60090 | None:intergenic | 40.0% |
| ACTTGCTTGCCTTCTCAAAA+TGG | - | tig0024950:60089-60108 | Msa1421380:CDS | 40.0% | |
| TGCTTGCCTTCTCAAAATGG+AGG | - | tig0024950:60092-60111 | Msa1421380:CDS | 45.0% | |
| TTGCCTTCTCAAAATGGAGG+TGG | - | tig0024950:60095-60114 | Msa1421380:CDS | 45.0% | |
| ATGGAGGTGGACCTAACAAT+AGG | - | tig0024950:60108-60127 | Msa1421380:CDS | 45.0% | |
| !! | GGTCCACCTCCATTTTGAGA+AGG | + | tig0024950:60101-60120 | None:intergenic | 50.0% |
| GGTGGACCTAACAATAGGTG+TGG | - | tig0024950:60113-60132 | Msa1421380:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0024950 | gene | 59894 | 60157 | 59894 | ID=Msa1421380;Name=Msa1421380 |
| tig0024950 | mRNA | 59894 | 60157 | 59894 | ID=Msa1421380-mRNA-1;Parent=Msa1421380;Name=Msa1421380-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0|-1|0|1|-1|1|1|0|87 |
| tig0024950 | exon | 59894 | 60157 | 59894 | ID=Msa1421380-mRNA-1:exon:2074;Parent=Msa1421380-mRNA-1 |
| tig0024950 | CDS | 59894 | 60157 | 59894 | ID=Msa1421380-mRNA-1:cds;Parent=Msa1421380-mRNA-1 |
| Gene Sequence |
| Protein sequence |
