AlfalfaGEDB | Msa1432340

M. sativa cultivar zhongmu-4 / Msa1432340

KEGG: K04733
GO: -
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1432340	AES82726.1	95.490	510	18	3	1	510	40	544	0.0	1002

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1432340	sp\|Q9SA25\|WAKLG_ARATH	37.037	297	181	2	199	493	378	670	2.98e-56	203

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1432340	G7L302	95.490	510	18	3	1	510	40	544	0.0	1002

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0528160	Msa1432340	0.961326	1.425763e-119	-8.615850e-47
Msa0688750	Msa1432340	0.968733	4.242689e-129	-8.615850e-47
Msa1038030	Msa1432340	0.853459	2.356973e-61	-8.615850e-47
Msa1130080	Msa1432340	0.915010	9.772639e-85	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1432340	MtrunA17_Chr7g0275121	95.490	510	18	3	1	510	40	544	0.0	1002
Msa1432340	MtrunA17_Chr6g0451071	37.183	355	211	5	147	496	2	349	1.64e-70	229
Msa1432340	MtrunA17_Chr7g0248904	37.979	287	174	3	199	482	350	635	4.49e-57	201
Msa1432340	MtrunA17_Chr4g0069251	35.522	335	194	5	197	510	165	498	4.34e-56	195
Msa1432340	MtrunA17_Chr2g0314901	35.244	349	212	5	139	480	600	941	1.03e-55	201
Msa1432340	MtrunA17_Chr5g0403051	38.339	313	183	5	181	484	296	607	1.15e-55	197
Msa1432340	MtrunA17_Chr7g0262521	38.776	294	175	3	199	487	334	627	3.16e-55	197
Msa1432340	MtrunA17_Chr6g0484231	36.280	328	193	6	194	508	34	358	3.20e-55	189
Msa1432340	MtrunA17_Chr7g0248881	37.631	287	175	3	199	482	343	628	4.40e-55	196
Msa1432340	MtrunA17_Chr8g0374031	38.170	317	189	5	199	510	325	639	6.48e-55	196

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1432340	AT1G16260.1	37.037	297	181	2	199	493	378	670	3.02e-57	203
Msa1432340	AT1G16260.2	37.037	297	181	2	199	493	378	670	3.02e-57	203
Msa1432340	AT1G16260.3	37.037	297	181	2	199	493	369	661	3.13e-57	202
Msa1432340	AT1G56720.3	37.748	302	176	4	197	494	165	458	5.47e-57	197
Msa1432340	AT1G56720.1	37.748	302	176	4	197	494	165	458	5.47e-57	197
Msa1432340	AT1G56720.2	37.748	302	176	4	197	494	165	458	5.47e-57	197
Msa1432340	AT1G56720.4	37.748	302	176	4	197	494	165	458	5.47e-57	197
Msa1432340	AT1G61860.1	38.667	300	176	5	217	509	91	389	3.91e-56	192
Msa1432340	AT2G42960.3	38.062	289	176	1	185	470	157	445	1.04e-55	194
Msa1432340	AT2G42960.5	38.062	289	176	1	185	470	157	445	1.04e-55	194

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Find 118 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGATAAACCTTGGACGC+GGG	0.536874	tig0027080:-43781	Msa1432340:CDS
AAACAGATAGCTACTTGGTC+TGG	0.466838	tig0027080:-43857	Msa1432340:CDS
AAAGAGGATATAACCAAATG+GGG	0.650234	tig0027080:-44953	None:intergenic
AAAGGGACAAGTTGATAAGG+AGG	0.638792	tig0027080:-42796	Msa1432340:CDS
AAATGGGGCAGTGGCAGTGA+TGG	0.530740	tig0027080:-44938	Msa1432340:five_prime_UTR
AACAGCCAAACAAGCAAACA+AGG	0.585721	tig0027080:+43621	None:intergenic
AACAGCTAGTAAAGATAAAT+TGG	0.245513	tig0027080:+44715	None:intergenic
AACCTTGGACGCGGGAGTGC+AGG	0.467218	tig0027080:-43773	Msa1432340:CDS
AACGCAAGAATTTGTCAGCA+AGG	0.589922	tig0027080:+44480	None:intergenic
AACTGGACGTGCTTCTTAAC+AGG	0.280805	tig0027080:+43881	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGAACTACTGACACAATAT+AGG	-	tig0027080:42641-42660	Msa1432340:intron	30.0%
TGAACTACTGACACAATATA+GGG	-	tig0027080:42642-42661	Msa1432340:intron	30.0%
TACATAGGTGAAAATAGACT+AGG	-	tig0027080:42806-42825	Msa1432340:CDS	30.0%
AAACCTTAAAAATGAGCCTA+TGG	+	tig0027080:42900-42919	None:intergenic	30.0%
ATCAATGAAGAAACTTGTGT+TGG	-	tig0027080:43041-43060	Msa1432340:CDS	30.0%
GTGAATGATTGATTGATCAA+GGG	+	tig0027080:43516-43535	None:intergenic	30.0%
AGTGAATGATTGATTGATCA+AGG	+	tig0027080:43517-43536	None:intergenic	30.0%
TATCTCCATCATCATATAGA+GGG	-	tig0027080:43662-43681	Msa1432340:CDS	30.0%
TAAGTGTGATGAAGTTAATC+TGG	-	tig0027080:43711-43730	Msa1432340:CDS	30.0%
TCCAAAACTATATACATCAC+TGG	+	tig0027080:43971-43990	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0027080	gene	42078	44960	42078	ID=Msa1432340;Name=Msa1432340
tig0027080	mRNA	42078	44960	42078	ID=Msa1432340-mRNA-1;Parent=Msa1432340;Name=Msa1432340-mRNA-1;_AED=0.03;_eAED=0.03;_QI=75\|1\|1\|1\|0.8\|0.66\|6\|435\|510
tig0027080	exon	42078	42575	42078	ID=Msa1432340-mRNA-1:exon:8040;Parent=Msa1432340-mRNA-1
tig0027080	exon	42674	42874	42674	ID=Msa1432340-mRNA-1:exon:8039;Parent=Msa1432340-mRNA-1
tig0027080	exon	42970	43230	42970	ID=Msa1432340-mRNA-1:exon:8038;Parent=Msa1432340-mRNA-1
tig0027080	exon	43329	43444	43329	ID=Msa1432340-mRNA-1:exon:8037;Parent=Msa1432340-mRNA-1
tig0027080	exon	43549	44001	43549	ID=Msa1432340-mRNA-1:exon:8036;Parent=Msa1432340-mRNA-1
tig0027080	exon	44447	44960	44447	ID=Msa1432340-mRNA-1:exon:8035;Parent=Msa1432340-mRNA-1
tig0027080	five_prime_UTR	44886	44960	44886	ID=Msa1432340-mRNA-1:five_prime_utr;Parent=Msa1432340-mRNA-1
tig0027080	CDS	44447	44885	44447	ID=Msa1432340-mRNA-1:cds;Parent=Msa1432340-mRNA-1
tig0027080	CDS	43549	44001	43549	ID=Msa1432340-mRNA-1:cds;Parent=Msa1432340-mRNA-1
tig0027080	CDS	43329	43444	43329	ID=Msa1432340-mRNA-1:cds;Parent=Msa1432340-mRNA-1
tig0027080	CDS	42970	43230	42970	ID=Msa1432340-mRNA-1:cds;Parent=Msa1432340-mRNA-1
tig0027080	CDS	42674	42874	42674	ID=Msa1432340-mRNA-1:cds;Parent=Msa1432340-mRNA-1
tig0027080	CDS	42513	42575	42513	ID=Msa1432340-mRNA-1:cds;Parent=Msa1432340-mRNA-1
tig0027080	three_prime_UTR	42078	42512	42078	ID=Msa1432340-mRNA-1:three_prime_utr;Parent=Msa1432340-mRNA-1

Gene Sequence

>Msa1432340

ATGGCCTCACAAGTCCGTAACACAAAAGGACAAATGTTCCTTTCTCAAGAACAATGGCAAAGTTGCAACCAATCATTTCATCCACAACCAGGGATGTCACTCTCTTCATGTGGCTTTGATAACTTTTACTTTGGTAGCTCTCAATGCTCCAATTTATCTTTACTAGCTGTTCAGAACTCGCAAAAGTACTCAGATGCATTGAATCAATGCAGCCACTTCGACCTTTCGTTTGATGAGTCGTGTGAGGATTGTACAACTGCAATATTGGATCTGAGAAATAGTTTGTATGACGCAACAGGGCCGGACAGCAATGATATTGAGAGAGCCATTTGCGGAGTAGCAGCTCTTGTTGCTGTTGCATTTGAGCAACAAAATGATCCTTTCCTTGCTGACAAATTCTTGCGTTGCTTGCCACCTCCAGCAGCAGTACATGACAAGAAATCATCAATGAAGAAACTTGTGTTGGCAATGTTGGTGATGGCTATCTTAGCATTATTGATTATTGCTTTTCTGGTAAAATGTGTATCAAAGAAGAAGCCTGTTAAGAAGCACGTCCAGTTGAAACAGATAGCTACTTGGTCTGGACTGTACTGGTTCACAAAAACTGAAATTGAAAATGCCATGAATTTTGAAAATGAAAAGATAAACCTTGGACGCGGGAGTGCAGGTGAAGTGTTTAGAGGTGTTTTGCCAAGTGGTCAAGTTGTGGCCATCAAGCATTTGACAAAGAGTAATACCTCTAGTTCTGATTCTTTCACTCGAGAACTTGCTGGTCTTTCTAGACTTCGTCATCCAAACCTTGTTTGCTTGTTTGGCTGTTGTATGGAAGATGGCGAAAGATATCTCGTCTACGAGTTCTGTGCCAATGGAAATCTTGCTCAACATCTCTTGAGAAGAGACAGCCATTTGACATGGGAAGCAAGAGTGAAGATTTTGAGAGAATGTTCATTTGCGCTTAAGTATCTCCATCATCATATAGAGGGTTGTGTTGTCCATAGAGATATCAAGCTTACAAACATTCTTTTGACTGAGAAATACGAAGCCAAACTGTCGGATTTTGGATTGTCAAGGATGATGGGGATGGAAGAGAGCAAGGTTTTTACTGATGTTAGAGGAACTATAGGTTACATGGATCCAGAGTACATGAGTAATGCCAAGTTAACTTGTGCCAGTGATGTATATAGTTTTGGAATTGTTGCTTTGCAAATTCTGTCAGGACAGAAAGTCATAGAGTTGGATCTTGATGCAAGAGATCAGCTCACCAGAAAGGCAAGAGATGTGAGTATGGGAAAGCGTCCATTGTCGGATTTTGTAGACTCGCGACTAAAGGGACAAGTTGATAAGGAGGACTTTGGAGCTATTTTACAGATTGCAGTACTTTGTGTTGCCAAATCAAGCACAGGTCGGCCACCAATTGAGGTTGTTTTTGACGAATTGGACAAAGTCTATAGGGACATTGAGGCGCGCAAGAAGGCAACCCCAACAACATCAACAACTACATCAACATCAACATCAACATCTAGCTCTAACTGA

Protein sequence

>Msa1432340

MASQVRNTKGQMFLSQEQWQSCNQSFHPQPGMSLSSCGFDNFYFGSSQCSNLSLLAVQNSQKYSDALNQCSHFDLSFDESCEDCTTAILDLRNSLYDATGPDSNDIERAICGVAALVAVAFEQQNDPFLADKFLRCLPPPAAVHDKKSSMKKLVLAMLVMAILALLIIAFLVKCVSKKKPVKKHVQLKQIATWSGLYWFTKTEIENAMNFENEKINLGRGSAGEVFRGVLPSGQVVAIKHLTKSNTSSSDSFTRELAGLSRLRHPNLVCLFGCCMEDGERYLVYEFCANGNLAQHLLRRDSHLTWEARVKILRECSFALKYLHHHIEGCVVHRDIKLTNILLTEKYEAKLSDFGLSRMMGMEESKVFTDVRGTIGYMDPEYMSNAKLTCASDVYSFGIVALQILSGQKVIELDLDARDQLTRKARDVSMGKRPLSDFVDSRLKGQVDKEDFGAILQIAVLCVAKSSTGRPPIEVVFDELDKVYRDIEARKKATPTTSTTTSTSTSTSSSN*