AlfalfaGEDB | Msa1443730

M. sativa cultivar zhongmu-4 / Msa1443730

KEGG: -
GO: GO:0000902 GO:0000904 GO:0000976 GO:0000981 GO:0001046 GO:0001047 GO:0001067 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010015 GO:0010033 GO:0010053 GO:0010054 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0021700 GO:0022622 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0032989 GO:0034641 GO:0034645 GO:0034654 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048522 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0097659 GO:0099402 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1905392 GO:1990837 GO:2000112 GO:2001141
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1443730	sp\|Q7XHI9\|BH084_ARATH	41.390	331	146	13	10	305	6	323	3.81e-60	198

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1443730	G7K0X1	91.892	333	18	3	1	326	1	331	0.0	619

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TFs/TRs:

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Gene ID	Type	Classification
Msa1443730	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0581000	Msa1443730	0.850589	1.540242e-60	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1443730	MtrunA17_Chr5g0393721	91.892	333	18	3	1	326	1	331	0.0	619
Msa1443730	MtrunA17_Chr1g0207301	48.980	343	126	12	1	309	51	378	1.21e-82	255
Msa1443730	MtrunA17_Chr1g0207311	48.243	313	130	8	1	308	1	286	5.97e-78	239
Msa1443730	MtrunA17_Chr3g0134741	50.909	220	92	7	93	307	111	319	2.05e-56	185
Msa1443730	MtrunA17_Chr1g0152651	58.491	159	53	4	139	294	201	349	2.08e-44	155
Msa1443730	MtrunA17_Chr5g0435021	62.500	72	27	0	216	287	215	286	1.01e-25	104
Msa1443730	MtrunA17_Chr4g0074161	60.000	65	26	0	218	282	205	269	9.55e-19	86.3
Msa1443730	MtrunA17_Chr8g0384141	66.071	56	19	0	228	283	123	178	1.33e-18	84.0
Msa1443730	MtrunA17_Chr3g0134001	61.111	54	21	0	228	281	180	233	7.19e-17	79.3
Msa1443730	MtrunA17_Chr7g0243041	60.000	55	22	0	226	280	33	87	8.20e-17	76.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1443730	AT2G14760.1	41.390	331	146	13	10	305	6	323	3.87e-61	198
Msa1443730	AT4G33880.1	39.828	349	147	11	10	301	6	348	1.53e-58	192
Msa1443730	AT2G14760.3	40.580	345	142	15	10	305	6	336	1.93e-57	189
Msa1443730	AT1G27740.1	67.544	114	36	1	190	302	137	250	1.71e-47	160
Msa1443730	AT5G43175.1	54.140	157	53	5	155	302	69	215	8.51e-45	152
Msa1443730	AT5G37800.1	70.000	60	18	0	228	287	210	269	2.54e-24	100
Msa1443730	AT1G66470.1	71.667	60	17	0	228	287	204	263	2.60e-24	100
Msa1443730	AT2G24260.2	63.077	65	24	0	218	282	34	98	3.70e-21	91.3
Msa1443730	AT2G24260.1	63.077	65	24	0	218	282	137	201	2.43e-20	90.5
Msa1443730	AT4G30980.1	63.077	65	24	0	218	282	129	193	2.70e-20	90.1

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Find 62 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGACTTACTCTTGCATAG+AGG	0.624768	tig0030514:-25488	None:intergenic
AAAGTTACCTTAGTTCCATT+GGG	0.395654	tig0030514:-25901	None:intergenic
AACGTCTGATCCTGAGAACC+AGG	0.576280	tig0030514:-25321	None:intergenic
AACTTGTGCAGTAACAATTG+TGG	0.601327	tig0030514:-24815	None:intergenic
AAGATCTAGGAGCTCAAATG+AGG	0.582801	tig0030514:+25065	Msa1443730:CDS
AAGTTACCTTAGTTCCATTG+GGG	0.630156	tig0030514:-25900	None:intergenic
AATTCTTCTCATGAGCTACG+TGG	0.695539	tig0030514:+25372	Msa1443730:CDS
ACAAGAGGAATGTCAAATGT+AGG	0.531678	tig0030514:+25253	Msa1443730:CDS
ACAATTGTGGAATCATGTGG+TGG	0.728994	tig0030514:-24802	None:intergenic
ACCACAAGCGCAGAAAATTC+AGG	0.383330	tig0030514:+25039	Msa1443730:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CATAGAAATATAGTCAAAGC+TGG	-	tig0030514:24887-24906	None:intergenic	30.0%
AAAACGAAATGCATATCACT+TGG	-	tig0030514:25160-25179	None:intergenic	30.0%
AAAATCCAAACCATAAACTG+TGG	-	tig0030514:25186-25205	None:intergenic	30.0%
TTCAATCAAGCTCGATTAAA+AGG	+	tig0030514:25688-25707	Msa1443730:intron	30.0%
GTGGATGTTAATTGTTAAGT+TGG	+	tig0030514:25718-25737	Msa1443730:intron	30.0%
AAAGTTACCTTAGTTCCATT+GGG	-	tig0030514:25904-25923	None:intergenic	30.0%
AATATATAGTCTGAATGGCA+TGG	-	tig0030514:25938-25957	None:intergenic	30.0%
CAACTTCAAATTAAGGTTTG+AGG	+	tig0030514:26068-26087	Msa1443730:intron	30.0%
GATCCCTATATGAATTTGAA+TGG	+	tig0030514:26150-26169	Msa1443730:intron	30.0%
TTCATTCCATTGTAAGCAAT+GGG	-	tig0030514:26214-26233	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0030514	gene	24640	26321	24640	ID=Msa1443730;Name=Msa1443730
tig0030514	mRNA	24640	26321	24640	ID=Msa1443730-mRNA-1;Parent=Msa1443730;Name=Msa1443730-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0\|0\|0\|1\|1\|1\|5\|0\|326
tig0030514	exon	24640	25086	24640	ID=Msa1443730-mRNA-1:exon:14040;Parent=Msa1443730-mRNA-1
tig0030514	exon	25243	25500	25243	ID=Msa1443730-mRNA-1:exon:14041;Parent=Msa1443730-mRNA-1
tig0030514	exon	25850	25915	25850	ID=Msa1443730-mRNA-1:exon:14042;Parent=Msa1443730-mRNA-1
tig0030514	exon	26017	26082	26017	ID=Msa1443730-mRNA-1:exon:14043;Parent=Msa1443730-mRNA-1
tig0030514	exon	26178	26321	26178	ID=Msa1443730-mRNA-1:exon:14044;Parent=Msa1443730-mRNA-1
tig0030514	CDS	24640	25086	24640	ID=Msa1443730-mRNA-1:cds;Parent=Msa1443730-mRNA-1
tig0030514	CDS	25243	25500	25243	ID=Msa1443730-mRNA-1:cds;Parent=Msa1443730-mRNA-1
tig0030514	CDS	25850	25915	25850	ID=Msa1443730-mRNA-1:cds;Parent=Msa1443730-mRNA-1
tig0030514	CDS	26017	26082	26017	ID=Msa1443730-mRNA-1:cds;Parent=Msa1443730-mRNA-1
tig0030514	CDS	26178	26321	26178	ID=Msa1443730-mRNA-1:cds;Parent=Msa1443730-mRNA-1

Gene Sequence

>Msa1443730

ATGGAACCTACTGAGCTTATTTCTCAAGAATGGACTTCTCTCAGTGGACTCTACACTGCTGAGGAATCTGACTTTATGAACCAGTTACTTGGTAACTCCTCATTCTCATCACAACATTTCTATCAAAATTCCAATTTGTTGGGTACTGAAACTGCATTTTGGCCACCACATGATTCCACAATTGTTACTGCACAAGTTCCTACCTCAACTTCTGATACTAGTTGCTTCAACAACAATCCAGTAACCAGCTTTGACTATATTTCTATGGGTCTTTCCATAGATCATTCCAAATTCACTCCATGTACAACACAATCTGATGAACAAGTTCTAGCTAACAAAAACTTGCAGGCCCGAAAATCAGAACGTGAAGTACTAGTTTCTGAACCAGAAGAGGATAAAACCACAAGCGCAGAAAATTCAGGAAAAAGATCTAGGAGCTCAAATGAGGTCCCAAAGAACAAGAGGAATGTCAAATGTAGGAAGATTATAAGATGTGCTTCTATAAGCACTGAAGAGAATACAAGTCCTGGTTCTCAGGATCAGACGTTAAGCGGTTATTGTTCAGAGGATGAGTCCAATTCTTCTCATGAGCTACGTGGAAGGGAATCTTCAAGTTTGAGCCAGAATGATTCTACGGCTAAATTAAGTGGAAAATCAAGATCTAGTAGAGGTCCTGCTACTGATCCACAGAGCCTCTATGCAAGAAAAAGAAGAGAAAGAATAAATGAAAGGTTGAAAATCCTACAAAACCTTGTCCCCAATGGAACTAAGGTGGATATCAGCACCATGCTTGAGGAAGCTGTCCAATATGTGAAGTTTTTACAACTTCAAATTAAGCTTCTAAGCTCTGATGACATGTGGATGTACTCTCCCATTGCTTACAATGGAATGAACATTGGACTTGAACTTGGTATTACCCCAACAAAAGAAACAAAGGATATGCATAACAACATACCAATAAAAGAGAATGCAGCAGTTTAA

Protein sequence

>Msa1443730

MEPTELISQEWTSLSGLYTAEESDFMNQLLGNSSFSSQHFYQNSNLLGTETAFWPPHDSTIVTAQVPTSTSDTSCFNNNPVTSFDYISMGLSIDHSKFTPCTTQSDEQVLANKNLQARKSEREVLVSEPEEDKTTSAENSGKRSRSSNEVPKNKRNVKCRKIIRCASISTEENTSPGSQDQTLSGYCSEDESNSSHELRGRESSSLSQNDSTAKLSGKSRSSRGPATDPQSLYARKRRERINERLKILQNLVPNGTKVDISTMLEEAVQYVKFLQLQIKLLSSDDMWMYSPIAYNGMNIGLELGITPTKETKDMHNNIPIKENAAV*