AlfalfaGEDB | Msa1444570

M. sativa cultivar zhongmu-4 / Msa1444570

KEGG: K07198
GO: GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010646 GO:0010648 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018107 GO:0018193 GO:0018209 GO:0018210 GO:0019538 GO:0023051 GO:0023052 GO:0023057 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1901701 GO:1905957 GO:1905958
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1444570	sp\|Q7X996\|CIPK2_ORYSJ	74.026	77	20	0	1	77	354	430	7.84e-34	124

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1444570	I3SEU7	98.039	102	2	0	1	102	19	120	9.11e-65	200

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1065000	Msa1444570	0.806059	9.886879e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1444570	MtrunA17_Chr7g0244521	98.039	102	2	0	1	102	361	462	2.85e-65	203
Msa1444570	MtrunA17_Chr4g0013131	76.289	97	23	0	1	97	356	452	1.04e-47	157
Msa1444570	MtrunA17_Chr5g0432471	68.750	80	25	0	1	80	379	458	1.12e-33	120
Msa1444570	MtrunA17_Chr3g0107011	70.130	77	23	0	1	77	362	438	6.77e-33	118
Msa1444570	MtrunA17_Chr1g0205421	51.685	89	43	0	1	89	362	450	2.39e-22	89.7
Msa1444570	MtrunA17_Chr2g0333821	47.500	80	42	0	1	80	365	444	3.79e-22	89.0
Msa1444570	MtrunA17_Chr3g0114511	48.810	84	43	0	1	84	363	446	3.28e-21	86.3
Msa1444570	MtrunA17_Chr1g0150321	57.812	64	27	0	17	80	360	423	1.35e-20	84.7
Msa1444570	MtrunA17_Chr4g0001331	46.154	78	42	0	3	80	372	449	2.11e-20	84.3
Msa1444570	MtrunA17_Chr4g0074111	46.667	75	39	1	1	75	310	383	2.43e-17	75.5
Msa1444570	MtrunA17_Chr2g0284011	44.595	74	40	1	6	79	368	440	2.30e-15	70.1
Msa1444570	MtrunA17_Chr8g0379091	50.725	69	33	1	5	73	401	468	3.44e-15	69.3
Msa1444570	MtrunA17_Chr2g0284031	45.205	73	39	1	1	73	394	465	1.51e-14	67.8
Msa1444570	MtrunA17_Chr8g0346081	48.333	60	30	1	17	76	374	432	3.42e-14	66.6
Msa1444570	MtrunA17_Chr8g0346071	48.333	60	30	1	17	76	373	431	6.14e-14	65.9
Msa1444570	MtrunA17_Chr2g0304111	46.154	65	34	1	11	75	381	444	5.00e-13	63.2
Msa1444570	MtrunA17_Chr5g0441011	36.585	82	50	2	3	84	359	438	2.87e-12	61.2
Msa1444570	MtrunA17_Chr2g0284021	43.077	65	36	1	6	70	68	131	3.04e-11	55.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1444570	AT5G07070.1	56.383	94	39	1	1	94	359	450	8.79e-32	115
Msa1444570	AT5G58380.1	62.025	79	30	0	5	83	375	453	1.30e-30	112
Msa1444570	AT5G45820.1	56.757	74	31	1	6	79	353	425	6.48e-23	91.7
Msa1444570	AT5G10930.1	48.750	80	41	0	1	80	357	436	3.78e-22	89.4
Msa1444570	AT5G25110.1	47.500	80	42	0	1	80	386	465	2.17e-19	81.6
Msa1444570	AT2G25090.1	44.304	79	44	0	5	83	371	449	3.81e-19	80.9
Msa1444570	AT4G30960.1	47.692	65	34	0	11	75	372	436	4.10e-16	72.4
Msa1444570	AT1G29230.1	47.297	74	38	1	1	74	431	503	3.22e-15	70.1
Msa1444570	AT1G01140.1	42.105	76	43	1	11	86	373	447	7.30e-15	68.9
Msa1444570	AT1G01140.2	42.105	76	43	1	11	86	375	449	7.38e-15	68.9
Msa1444570	AT1G01140.3	40.506	79	44	1	11	86	373	451	1.20e-14	68.2
Msa1444570	AT4G18700.1	46.575	73	38	1	1	73	384	455	3.86e-14	67.0
Msa1444570	AT2G34180.1	40.000	85	49	2	1	85	411	493	2.35e-13	64.7
Msa1444570	AT1G30270.3	41.176	68	39	1	17	84	298	364	7.76e-13	63.2
Msa1444570	AT5G45810.1	44.595	74	39	2	1	73	388	460	8.12e-13	63.2
Msa1444570	AT1G30270.1	41.176	68	39	1	17	84	395	461	8.69e-13	63.2
Msa1444570	AT5G01810.3	43.590	78	39	2	1	78	304	376	8.97e-13	62.8
Msa1444570	AT1G30270.2	41.176	68	39	1	17	84	395	461	1.07e-12	62.8
Msa1444570	AT5G01810.1	43.590	78	39	2	1	78	349	421	1.98e-12	62.0
Msa1444570	AT5G01810.2	43.590	78	39	2	1	78	349	421	1.98e-12	62.0
Msa1444570	AT2G26980.3	40.541	74	42	2	2	75	359	430	3.10e-12	61.6
Msa1444570	AT2G26980.4	40.541	74	42	2	2	75	369	440	3.37e-12	61.2
Msa1444570	AT5G21326.1	36.986	73	44	2	3	75	359	429	9.14e-12	60.1

Find 21 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGATGGAGGTTTGTTAAAGT+TGG	0.380754	tig0030745:-47128	Msa1444570:CDS
GGAGATTAGACCAGCACTTA+AGG	0.433631	tig0030745:-46972	Msa1444570:CDS
TTAAGGACATTGTGTGGAAC+TGG	0.442161	tig0030745:-46955	Msa1444570:CDS
TGGAAGGATCAAAGGAAGGA+AGG	0.485757	tig0030745:-47108	Msa1444570:CDS
ACTTCTTAAGTTCCACAAGA+TGG	0.487934	tig0030745:+47027	None:intergenic
GTTAAAGTTGGAAGGATCAA+AGG	0.492131	tig0030745:-47116	Msa1444570:CDS
TACTCCTCACTTCCATCTTG+TGG	0.492933	tig0030745:-47047	Msa1444570:CDS
CCTCTTGCACTACTTCATGT+TGG	0.504306	tig0030745:+46901	None:intergenic
GGATCAAAGGAAGGAAGGAA+AGG	0.514975	tig0030745:-47103	Msa1444570:CDS
GGAGGTTTGTTAAAGTTGGA+AGG	0.515977	tig0030745:-47124	Msa1444570:CDS
CACACAATGTCCTTAAGTGC+TGG	0.534674	tig0030745:+46954	None:intergenic
ACTTCATGTTGGAGTTGCTG+AGG	0.575610	tig0030745:+46912	None:intergenic
GACATTGTGTGGAACTGGCA+AGG	0.577818	tig0030745:-46950	Msa1444570:CDS
AGGAGTAATCTCGAAAATCT+CGG	0.578411	tig0030745:+47055	None:intergenic
GATCAAAGGAAGGAAGGAAA+GGG	0.584306	tig0030745:-47102	Msa1444570:CDS
AAGTTGGAAGGATCAAAGGA+AGG	0.599122	tig0030745:-47112	Msa1444570:CDS
CCAACATGAAGTAGTGCAAG+AGG	0.615269	tig0030745:-46909	Msa1444570:CDS
GAAGTCTGATGGTGATACAG+TGG	0.633395	tig0030745:-47017	Msa1444570:CDS
GTGGAACTTAAGAAGTCTGA+TGG	0.654560	tig0030745:-47028	Msa1444570:CDS
CAGCACTTAAGGACATTGTG+TGG	0.659745	tig0030745:-46961	Msa1444570:CDS
AGTTCCACAAGATGGAAGTG+AGG	0.662383	tig0030745:+47035	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTGAAAGTAAAGAAAAAAGA+TGG	-	tig0030745:46890-46909	Msa1444570:CDS	20.0%
!	AAAGTAAAGAAAAAAGATGG+AGG	-	tig0030745:46893-46912	Msa1444570:CDS	25.0%
!!	AGATGGAGGTTTGTTAAAGT+TGG	-	tig0030745:46907-46926	Msa1444570:CDS	35.0%
	GTTAAAGTTGGAAGGATCAA+AGG	-	tig0030745:46919-46938	Msa1444570:CDS	35.0%
	AGGAGTAATCTCGAAAATCT+CGG	+	tig0030745:46975-46994	None:intergenic	35.0%
	ACTTCTTAAGTTCCACAAGA+TGG	+	tig0030745:47003-47022	None:intergenic	35.0%
!!	GGAGGTTTGTTAAAGTTGGA+AGG	-	tig0030745:46911-46930	Msa1444570:CDS	40.0%
	AAGTTGGAAGGATCAAAGGA+AGG	-	tig0030745:46923-46942	Msa1444570:CDS	40.0%
	GATCAAAGGAAGGAAGGAAA+GGG	-	tig0030745:46933-46952	Msa1444570:CDS	40.0%
	GTGGAACTTAAGAAGTCTGA+TGG	-	tig0030745:47007-47026	Msa1444570:CDS	40.0%
!	GTATGAGAAGCTTTTGCAAC+AGG	-	tig0030745:47042-47061	Msa1444570:CDS	40.0%
	TTAAGGACATTGTGTGGAAC+TGG	-	tig0030745:47080-47099	Msa1444570:CDS	40.0%
	TGGAAGGATCAAAGGAAGGA+AGG	-	tig0030745:46927-46946	Msa1444570:CDS	45.0%
	GGATCAAAGGAAGGAAGGAA+AGG	-	tig0030745:46932-46951	Msa1444570:CDS	45.0%
	TACTCCTCACTTCCATCTTG+TGG	-	tig0030745:46988-47007	Msa1444570:CDS	45.0%
	AGTTCCACAAGATGGAAGTG+AGG	+	tig0030745:46995-47014	None:intergenic	45.0%
!	GAAGTCTGATGGTGATACAG+TGG	-	tig0030745:47018-47037	Msa1444570:CDS	45.0%
	GGAGATTAGACCAGCACTTA+AGG	-	tig0030745:47063-47082	Msa1444570:CDS	45.0%
	CACACAATGTCCTTAAGTGC+TGG	+	tig0030745:47076-47095	None:intergenic	45.0%
	CAGCACTTAAGGACATTGTG+TGG	-	tig0030745:47074-47093	Msa1444570:CDS	45.0%
	ACTTCATGTTGGAGTTGCTG+AGG	+	tig0030745:47118-47137	None:intergenic	45.0%
	CCTCTTGCACTACTTCATGT+TGG	+	tig0030745:47129-47148	None:intergenic	45.0%
	CCAACATGAAGTAGTGCAAG+AGG	-	tig0030745:47126-47145	Msa1444570:CDS	45.0%
	GACATTGTGTGGAACTGGCA+AGG	-	tig0030745:47085-47104	Msa1444570:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
tig0030745	gene	46872	47177	46872	ID=Msa1444570;Name=Msa1444570
tig0030745	mRNA	46872	47177	46872	ID=Msa1444570-mRNA-1;Parent=Msa1444570;Name=Msa1444570-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|102
tig0030745	exon	46872	47177	46872	ID=Msa1444570-mRNA-1:exon:14531;Parent=Msa1444570-mRNA-1
tig0030745	CDS	46872	47177	46872	ID=Msa1444570-mRNA-1:cds;Parent=Msa1444570-mRNA-1

Gene Sequence	>Msa1444570 ATTTGCAAGTGTCTTCAATTGAAAGTAAAGAAAAAAGATGGAGGTTTGTTAAAGTTGGAAGGATCAAAGGAAGGAAGGAAAGGGACTCTTAATATTGATGCCGAGATTTTCGAGATTACTCCTCACTTCCATCTTGTGGAACTTAAGAAGTCTGATGGTGATACAGTGGAGTATGAGAAGCTTTTGCAACAGGAGATTAGACCAGCACTTAAGGACATTGTGTGGAACTGGCAAGGAGAACAACCTCAGCAACTCCAACATGAAGTAGTGCAAGAGGAGCAACAATCATCTCTTACTGATCAAATT
Protein sequence	>Msa1444570 ICKCLQLKVKKKDGGLLKLEGSKEGRKGTLNIDAEIFEITPHFHLVELKKSDGDTVEYEKLLQQEIRPALKDIVWNWQGEQPQQLQHEVVQEEQQSSLTDQI