AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1448580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1448580 MtrunA17_Chr4g0057361 98.584 353 5 0 1 353 1 353 0.0 723
Msa1448580 MtrunA17_Chr4g0057931 84.348 345 51 3 5 348 2 344 0.0 588
Msa1448580 MtrunA17_Chr3g0132211 73.262 374 70 7 1 353 1 365 0.0 538
Msa1448580 MtrunA17_Chr2g0302241 70.386 233 46 5 1 218 1 225 1.20e-104 306
Msa1448580 MtrunA17_Chr2g0302251 76.000 75 12 1 285 353 1 75 1.59e-35 124
Msa1448580 MtrunA17_Chr8g0374091 27.919 197 130 5 159 343 181 377 2.73e-17 83.6
Msa1448580 MtrunA17_Chr8g0374071 28.426 197 129 5 159 343 181 377 2.79e-17 83.6
Msa1448580 MtrunA17_Chr1g0203981 29.032 186 117 7 174 345 174 358 1.62e-14 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1448580 AT4G37100.1 27.397 219 147 6 137 343 164 382 4.95e-18 85.9
Msa1448580 AT2G23520.1 27.397 219 147 6 137 343 160 378 1.31e-17 84.7
Msa1448580 AT5G66950.1 29.545 176 112 5 180 343 201 376 4.24e-16 80.1
Msa1448580 AT5G51920.1 26.275 255 155 10 106 345 115 351 3.98e-15 77.0
Msa1448580 AT4G22980.1 22.426 272 185 7 91 345 68 330 2.20e-12 68.6

Find 92 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATTAGATTTGCATATTC+TGG 0.163433 tig0032224:+13897 None:intergenic
TAAAGCTTTGTATCTATTAT+TGG 0.185223 tig0032224:+14702 None:intergenic
AACGGTTCCTCTTAGGTTAA+TGG 0.194786 tig0032224:-13135 Msa1448580:exon
AACTGTGTCCTATCCATTTC+AGG 0.228182 tig0032224:+13756 None:intergenic
TATTTGAACTACATAGTAAT+TGG 0.259149 tig0032224:-13074 Msa1448580:three_prime_UTR
CACCTCAAACGGTTCCTCTT+AGG 0.260239 tig0032224:-13142 Msa1448580:exon
CTTAAAGAAATTAAAGATAT+TGG 0.266407 tig0032224:+14536 None:intergenic
TTTATCTATCACTTAATCTA+TGG 0.267981 tig0032224:-14635 Msa1448580:five_prime_UTR
TCCATTTCTTTCTGTGTGAT+TGG 0.303785 tig0032224:+13951 None:intergenic
TCGCCAAGATCGCCTTCTTT+TGG 0.314541 tig0032224:-13789 Msa1448580:CDS
ACATAAGCAAATAGCCCTTT+TGG 0.327876 tig0032224:+13372 None:intergenic
AACTACTACATGACTATTCT+TGG 0.330101 tig0032224:-13534 Msa1448580:CDS
TGGAAGCAACCTTCCTGAAA+TGG 0.346397 tig0032224:-13769 Msa1448580:CDS
TTTGCCCGATGATGAGTATT+TGG 0.358355 tig0032224:-13625 Msa1448580:CDS
CTTCCAAAAGAAGGCGATCT+TGG 0.365150 tig0032224:+13786 None:intergenic
TCTGCTATTAATTTGTGTAT+AGG 0.365157 tig0032224:+14272 None:intergenic
TGATTGGACCTGACCATCTT+AGG 0.370123 tig0032224:+13967 None:intergenic
TGGCTTGATTTGAGGATTAG+AGG 0.376978 tig0032224:-13438 Msa1448580:CDS
GTCAAACAGTTTGGTTATGA+AGG 0.386145 tig0032224:-14240 Msa1448580:intron
CTAAGACATGGCTTGATTTG+AGG 0.386748 tig0032224:-13446 Msa1448580:CDS
TGAGCAGGAATCACCACATT+TGG 0.390093 tig0032224:+14029 None:intergenic
CTGAGGCTCATAGGAACTAT+TGG 0.395731 tig0032224:-13317 Msa1448580:CDS
ATATCTTTAATTTCTTTAAG+TGG 0.402128 tig0032224:-14533 Msa1448580:five_prime_UTR
CATATTCTGGATACTTTGCT+AGG 0.409869 tig0032224:+13910 None:intergenic
TAGTACTTGTTTCACATTGT+TGG 0.412272 tig0032224:-13101 Msa1448580:three_prime_UTR
ATTGGGTTTCTGAGGCTCAT+AGG 0.415008 tig0032224:-13326 Msa1448580:CDS
CTCAAATCAAGCCATGTCTT+AGG 0.417224 tig0032224:+13447 None:intergenic
CATTATCAATGAGGAGCCTT+CGG 0.420348 tig0032224:-13841 Msa1448580:CDS
AGAATATCAAGTAGTCTTGA+TGG 0.432250 tig0032224:+13729 None:intergenic
GAATACCAAGTCAAACAGTT+TGG 0.436290 tig0032224:-14249 Msa1448580:CDS
TCCAATCACACAGAAAGAAA+TGG 0.438510 tig0032224:-13952 Msa1448580:CDS
ACCCTCTCTGTTTGTTCCCA+TGG 0.445715 tig0032224:+14488 None:intergenic
AGACAACAAACTAAGAAATC+AGG 0.449055 tig0032224:+13228 None:intergenic
AAATGTAAGATTAGTCCAAA+AGG 0.451312 tig0032224:-13387 Msa1448580:CDS
TATCACTTAATCTATGGTTA+AGG 0.452574 tig0032224:-14629 Msa1448580:five_prime_UTR
AACCTAAGAGGAACCGTTTG+AGG 0.459963 tig0032224:+13140 None:intergenic
CATTCATCCATTAACCTAAG+AGG 0.460841 tig0032224:+13128 None:intergenic
TCCAACAGGAACTTTCTTAG+TGG 0.461813 tig0032224:+14295 None:intergenic
AATTTGTGTATAGGTCCAAC+AGG 0.463593 tig0032224:+14281 None:intergenic
GAATATGCAAATCTAATTGA+AGG 0.466896 tig0032224:-13894 Msa1448580:CDS
ATCATAGCATCCTTGTAACT+TGG 0.468532 tig0032224:+13591 None:intergenic
GGAAGGTTGCTTCCAAAAGA+AGG 0.470365 tig0032224:+13777 None:intergenic
AATGGTAGAAGATGAAGTTA+AGG 0.470920 tig0032224:-14318 Msa1448580:CDS
AGTGATAGATAAAGATCCTA+TGG 0.472319 tig0032224:+14645 None:intergenic
GACATTGACCTAAGATGGTC+AGG 0.473329 tig0032224:-13975 Msa1448580:CDS
TTAGGCAATATATCATGAAA+TGG 0.474165 tig0032224:-14336 Msa1448580:intron
CATAACCAAACTGTTTGACT+TGG 0.474370 tig0032224:+14244 None:intergenic
TAGTAGTTTCCCTTAACAAA+AGG 0.476225 tig0032224:+13549 None:intergenic
TGATGCATCTGCAATGGTTG+TGG 0.477900 tig0032224:-13283 Msa1448580:CDS
GGAACAAACAGAGAGGGTCT+TGG 0.479456 tig0032224:-14483 Msa1448580:CDS
AGAACCAAATACTCATCATC+GGG 0.504982 tig0032224:+13621 None:intergenic
GGCTTGATTTGAGGATTAGA+GGG 0.509192 tig0032224:-13437 Msa1448580:CDS
CTAATTGAAGGAGATGGAAG+TGG 0.511234 tig0032224:-13882 Msa1448580:CDS
GCTTATGTAGCAGATGTGAA+TGG 0.511879 tig0032224:-13357 Msa1448580:CDS
GGTATCGAGATGAAACTTCC+CGG 0.514241 tig0032224:+13687 None:intergenic
AATGTAAGATTAGTCCAAAA+GGG 0.515212 tig0032224:-13386 Msa1448580:CDS
GCAAATCTAATTGAAGGAGA+TGG 0.520214 tig0032224:-13888 Msa1448580:CDS
GATGCATCTGCAATGGTTGT+GGG 0.522582 tig0032224:-13282 Msa1448580:CDS
TCCGAGACATTGACCTAAGA+TGG 0.525636 tig0032224:-13980 Msa1448580:CDS
GCTTCAGCTATGATATGAGC+AGG 0.531260 tig0032224:+14014 None:intergenic
ATCTGCAATGGTTGTGGGAA+AGG 0.534566 tig0032224:-13277 Msa1448580:CDS
AAAATGCCAAAGAAAATCAC+AGG 0.540929 tig0032224:+14597 None:intergenic
GGCTACATGTTGGACTTCAT+AGG 0.543263 tig0032224:-13251 Msa1448580:CDS
TTTCTTCTATCATCTGACAT+TGG 0.547327 tig0032224:+13813 None:intergenic
CAATGGTTGTGGGAAAGGAT+AGG 0.550776 tig0032224:-13272 Msa1448580:CDS
TAAACCATGGTTAAGTCCAT+AGG 0.552536 tig0032224:-14661 Msa1448580:five_prime_UTR
TGTGAATGGGACAATGCATT+GGG 0.555918 tig0032224:-13343 Msa1448580:CDS
AAGAACCAAATACTCATCAT+CGG 0.557250 tig0032224:+13620 None:intergenic
CTTATGTAGCAGATGTGAAT+GGG 0.557481 tig0032224:-13356 Msa1448580:CDS
GACAATGCATTGGGTTTCTG+AGG 0.557691 tig0032224:-13334 Msa1448580:CDS
ACAAACAGAGAGGGTCTTGG+AGG 0.561611 tig0032224:-14480 Msa1448580:CDS
GTCAAGTTTCATTATCAATG+AGG 0.565150 tig0032224:-13850 Msa1448580:CDS
ATGTGAATGGGACAATGCAT+TGG 0.572793 tig0032224:-13344 Msa1448580:CDS
GCCACTAAGAAAGTTCCTGT+TGG 0.572806 tig0032224:-14296 Msa1448580:CDS
TTTGCTTGATGCATCTGCAA+TGG 0.577791 tig0032224:-13289 Msa1448580:CDS
CGAGATGAAACTTCCCGGAA+AGG 0.583188 tig0032224:+13692 None:intergenic
GCAACCTTCCTGAAATGGAT+AGG 0.584340 tig0032224:-13764 Msa1448580:CDS
ACCATCTTAGGTCAATGTCT+CGG 0.593089 tig0032224:+13979 None:intergenic
TATTCTTGGTGAACAACAAG+AGG 0.595252 tig0032224:-13520 Msa1448580:CDS
TTCTCATAGCACCTAAGACA+TGG 0.598496 tig0032224:-13458 Msa1448580:CDS
GGAAAGGATAGGCTACATGT+TGG 0.599899 tig0032224:-13261 Msa1448580:CDS
TCTGACATTGGTAATTCCGA+AGG 0.602549 tig0032224:+13825 None:intergenic
CAAGGATGCTATGATGTTGG+TGG 0.611622 tig0032224:-13583 Msa1448580:CDS
AGAGGGTCTTGGAGGATCTG+AGG 0.619322 tig0032224:-14472 Msa1448580:intron
AGATAATAATGTGTAAACCA+TGG 0.624975 tig0032224:-14674 Msa1448580:five_prime_UTR
ACCATGGGAACAAACAGAGA+GGG 0.637502 tig0032224:-14489 Msa1448580:CDS
AGATCCTATGGACTTAACCA+TGG 0.639514 tig0032224:+14657 None:intergenic
TTACAAGGATGCTATGATGT+TGG 0.644788 tig0032224:-13586 Msa1448580:CDS
GTGTCCTATCCATTTCAGGA+AGG 0.648688 tig0032224:+13760 None:intergenic
AAGGATGCTATGATGTTGGT+GGG 0.665370 tig0032224:-13582 Msa1448580:CDS
ACTTAAACAGAAACCAAATG+TGG 0.672506 tig0032224:-14042 Msa1448580:intron
TACCATGGGAACAAACAGAG+AGG 0.720750 tig0032224:-14490 Msa1448580:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTAAAGAAATTAAAGATAT+TGG + tig0032224:13251-13270 None:intergenic 15.0%
!!! ATATCTTTAATTTCTTTAAG+TGG - tig0032224:13251-13270 Msa1448580:CDS 15.0%
!! ACTAACAATAAACTTATAAT+TGG - tig0032224:13718-13737 Msa1448580:CDS 15.0%
!! CTTAAAGAAATTAAAGATAT+TGG + tig0032224:13251-13270 None:intergenic 15.0%
!!! ATATCTTTAATTTCTTTAAG+TGG - tig0032224:13251-13270 Msa1448580:CDS 15.0%
!! ACTAACAATAAACTTATAAT+TGG - tig0032224:13718-13737 Msa1448580:CDS 15.0%
!!! TAAAGCTTTGTATCTATTAT+TGG + tig0032224:13085-13104 None:intergenic 20.0%
!! TTTATCTATCACTTAATCTA+TGG - tig0032224:13149-13168 Msa1448580:CDS 20.0%
!!! TTTTTTAGTGTTTAACACTT+GGG + tig0032224:13228-13247 None:intergenic 20.0%
!! ATTGAAGAAAAATATTACCA+TGG - tig0032224:13279-13298 Msa1448580:CDS 20.0%
!! TTGAAGAAAAATATTACCAT+GGG - tig0032224:13280-13299 Msa1448580:CDS 20.0%
!! TATTTGAACTACATAGTAAT+TGG - tig0032224:14710-14729 Msa1448580:five_prime_UTR 20.0%
!!! TAAAGCTTTGTATCTATTAT+TGG + tig0032224:13085-13104 None:intergenic 20.0%
!! TTTATCTATCACTTAATCTA+TGG - tig0032224:13149-13168 Msa1448580:CDS 20.0%
!!! TTTTTTAGTGTTTAACACTT+GGG + tig0032224:13228-13247 None:intergenic 20.0%
!! ATTGAAGAAAAATATTACCA+TGG - tig0032224:13279-13298 Msa1448580:CDS 20.0%
!! TTGAAGAAAAATATTACCAT+GGG - tig0032224:13280-13299 Msa1448580:CDS 20.0%
!! TATTTGAACTACATAGTAAT+TGG - tig0032224:14710-14729 Msa1448580:five_prime_UTR 20.0%
! AGATAATAATGTGTAAACCA+TGG - tig0032224:13110-13129 Msa1448580:three_prime_UTR 25.0%
! TATCACTTAATCTATGGTTA+AGG - tig0032224:13155-13174 Msa1448580:CDS 25.0%
!!! GTTTTTTAGTGTTTAACACT+TGG + tig0032224:13229-13248 None:intergenic 25.0%
! GAAACAAACATGAATTGAAT+AGG + tig0032224:13368-13387 None:intergenic 25.0%
! ATCATGGTAAACTATATGTT+AGG - tig0032224:13430-13449 Msa1448580:CDS 25.0%
! TTAGGCAATATATCATGAAA+TGG - tig0032224:13448-13467 Msa1448580:CDS 25.0%
!!! TCTGCTATTAATTTGTGTAT+AGG + tig0032224:13515-13534 None:intergenic 25.0%
! TCAATTAGATTTGCATATTC+TGG + tig0032224:13890-13909 None:intergenic 25.0%
! GAATATGCAAATCTAATTGA+AGG - tig0032224:13890-13909 Msa1448580:CDS 25.0%
!!! TTATTTTGAGCTTGAATTTC+TGG + tig0032224:14121-14140 None:intergenic 25.0%
!!! AATAAAGTTTTGAAGCATTG+TGG - tig0032224:14136-14155 Msa1448580:intron 25.0%
!! AGAAAGTTATCCTTTTGTTA+AGG - tig0032224:14225-14244 Msa1448580:intron 25.0%
!! GAAAGTTATCCTTTTGTTAA+GGG - tig0032224:14226-14245 Msa1448580:intron 25.0%
!!! CAAAGAATTTGCTTCTTTTA+AGG - tig0032224:14294-14313 Msa1448580:CDS 25.0%
!! CTCAGTTAAGTCAAAATTTT+AGG - tig0032224:14371-14390 Msa1448580:intron 25.0%
!! AGTTAAGTCAAAATTTTAGG+AGG - tig0032224:14374-14393 Msa1448580:intron 25.0%
!! AAATGTAAGATTAGTCCAAA+AGG - tig0032224:14397-14416 Msa1448580:intron 25.0%
!! AATGTAAGATTAGTCCAAAA+GGG - tig0032224:14398-14417 Msa1448580:intron 25.0%
! AGATAATAATGTGTAAACCA+TGG - tig0032224:13110-13129 Msa1448580:three_prime_UTR 25.0%
! TATCACTTAATCTATGGTTA+AGG - tig0032224:13155-13174 Msa1448580:CDS 25.0%
!!! GTTTTTTAGTGTTTAACACT+TGG + tig0032224:13229-13248 None:intergenic 25.0%
! GAAACAAACATGAATTGAAT+AGG + tig0032224:13368-13387 None:intergenic 25.0%
! ATCATGGTAAACTATATGTT+AGG - tig0032224:13430-13449 Msa1448580:CDS 25.0%
! TTAGGCAATATATCATGAAA+TGG - tig0032224:13448-13467 Msa1448580:CDS 25.0%
!!! TCTGCTATTAATTTGTGTAT+AGG + tig0032224:13515-13534 None:intergenic 25.0%
! TCAATTAGATTTGCATATTC+TGG + tig0032224:13890-13909 None:intergenic 25.0%
! GAATATGCAAATCTAATTGA+AGG - tig0032224:13890-13909 Msa1448580:CDS 25.0%
!!! TTATTTTGAGCTTGAATTTC+TGG + tig0032224:14121-14140 None:intergenic 25.0%
!!! AATAAAGTTTTGAAGCATTG+TGG - tig0032224:14136-14155 Msa1448580:intron 25.0%
!! AGAAAGTTATCCTTTTGTTA+AGG - tig0032224:14225-14244 Msa1448580:intron 25.0%
!! GAAAGTTATCCTTTTGTTAA+GGG - tig0032224:14226-14245 Msa1448580:intron 25.0%
!!! CAAAGAATTTGCTTCTTTTA+AGG - tig0032224:14294-14313 Msa1448580:CDS 25.0%
!! CTCAGTTAAGTCAAAATTTT+AGG - tig0032224:14371-14390 Msa1448580:intron 25.0%
!! AGTTAAGTCAAAATTTTAGG+AGG - tig0032224:14374-14393 Msa1448580:intron 25.0%
!! AAATGTAAGATTAGTCCAAA+AGG - tig0032224:14397-14416 Msa1448580:intron 25.0%
!! AATGTAAGATTAGTCCAAAA+GGG - tig0032224:14398-14417 Msa1448580:intron 25.0%
AGTGATAGATAAAGATCCTA+TGG + tig0032224:13142-13161 None:intergenic 30.0%
! AAAAGACCTGTGATTTTCTT+TGG - tig0032224:13181-13200 Msa1448580:CDS 30.0%
AAAATGCCAAAGAAAATCAC+AGG + tig0032224:13190-13209 None:intergenic 30.0%
ATCTGCTAATTCTTGAATCA+TGG - tig0032224:13414-13433 Msa1448580:CDS 30.0%
AATGGTAGAAGATGAAGTTA+AGG - tig0032224:13466-13485 Msa1448580:CDS 30.0%
TGTAATATTACGTGCATGTT+TGG + tig0032224:13684-13703 None:intergenic 30.0%
ACTTAAACAGAAACCAAATG+TGG - tig0032224:13742-13761 Msa1448580:CDS 30.0%
GTCAAGTTTCATTATCAATG+AGG - tig0032224:13934-13953 Msa1448580:CDS 30.0%
TTTCTTCTATCATCTGACAT+TGG + tig0032224:13974-13993 None:intergenic 30.0%
! AGAATATCAAGTAGTCTTGA+TGG + tig0032224:14058-14077 None:intergenic 30.0%
CAACAAAAAATCATCCTTTC+CGG - tig0032224:14078-14097 Msa1448580:intron 30.0%
AACAAAAAATCATCCTTTCC+GGG - tig0032224:14079-14098 Msa1448580:intron 30.0%
AAGAACCAAATACTCATCAT+CGG + tig0032224:14167-14186 None:intergenic 30.0%
TAGTAGTTTCCCTTAACAAA+AGG + tig0032224:14238-14257 None:intergenic 30.0%
AACTACTACATGACTATTCT+TGG - tig0032224:14250-14269 Msa1448580:CDS 30.0%
!! TGCTTCTTTTAAGGAATCAA+AGG - tig0032224:14303-14322 Msa1448580:CDS 30.0%
AGACAACAAACTAAGAAATC+AGG + tig0032224:14559-14578 None:intergenic 30.0%
! AGAGACAAGTGATTTTTGAA+GGG + tig0032224:14600-14619 None:intergenic 30.0%
! AAGAGACAAGTGATTTTTGA+AGG + tig0032224:14601-14620 None:intergenic 30.0%
TTCAAAAATCACTTGTCTCT+TGG - tig0032224:14600-14619 Msa1448580:five_prime_UTR 30.0%
!! AAAAGTTTTGACACCTCAAA+CGG - tig0032224:14631-14650 Msa1448580:five_prime_UTR 30.0%
TAGTACTTGTTTCACATTGT+TGG - tig0032224:14683-14702 Msa1448580:five_prime_UTR 30.0%
AGTGATAGATAAAGATCCTA+TGG + tig0032224:13142-13161 None:intergenic 30.0%
! AAAAGACCTGTGATTTTCTT+TGG - tig0032224:13181-13200 Msa1448580:CDS 30.0%
AAAATGCCAAAGAAAATCAC+AGG + tig0032224:13190-13209 None:intergenic 30.0%
ATCTGCTAATTCTTGAATCA+TGG - tig0032224:13414-13433 Msa1448580:CDS 30.0%
AATGGTAGAAGATGAAGTTA+AGG - tig0032224:13466-13485 Msa1448580:CDS 30.0%
TGTAATATTACGTGCATGTT+TGG + tig0032224:13684-13703 None:intergenic 30.0%
ACTTAAACAGAAACCAAATG+TGG - tig0032224:13742-13761 Msa1448580:CDS 30.0%
GTCAAGTTTCATTATCAATG+AGG - tig0032224:13934-13953 Msa1448580:CDS 30.0%
TTTCTTCTATCATCTGACAT+TGG + tig0032224:13974-13993 None:intergenic 30.0%
! AGAATATCAAGTAGTCTTGA+TGG + tig0032224:14058-14077 None:intergenic 30.0%
CAACAAAAAATCATCCTTTC+CGG - tig0032224:14078-14097 Msa1448580:intron 30.0%
AACAAAAAATCATCCTTTCC+GGG - tig0032224:14079-14098 Msa1448580:intron 30.0%
AAGAACCAAATACTCATCAT+CGG + tig0032224:14167-14186 None:intergenic 30.0%
TAGTAGTTTCCCTTAACAAA+AGG + tig0032224:14238-14257 None:intergenic 30.0%
AACTACTACATGACTATTCT+TGG - tig0032224:14250-14269 Msa1448580:CDS 30.0%
!! TGCTTCTTTTAAGGAATCAA+AGG - tig0032224:14303-14322 Msa1448580:CDS 30.0%
AGACAACAAACTAAGAAATC+AGG + tig0032224:14559-14578 None:intergenic 30.0%
! AGAGACAAGTGATTTTTGAA+GGG + tig0032224:14600-14619 None:intergenic 30.0%
! AAGAGACAAGTGATTTTTGA+AGG + tig0032224:14601-14620 None:intergenic 30.0%
TTCAAAAATCACTTGTCTCT+TGG - tig0032224:14600-14619 Msa1448580:five_prime_UTR 30.0%
!! AAAAGTTTTGACACCTCAAA+CGG - tig0032224:14631-14650 Msa1448580:five_prime_UTR 30.0%
TAGTACTTGTTTCACATTGT+TGG - tig0032224:14683-14702 Msa1448580:five_prime_UTR 30.0%
TAAACCATGGTTAAGTCCAT+AGG - tig0032224:13123-13142 Msa1448580:three_prime_UTR 35.0%
AATTTGTGTATAGGTCCAAC+AGG + tig0032224:13506-13525 None:intergenic 35.0%
GAATACCAAGTCAAACAGTT+TGG - tig0032224:13535-13554 Msa1448580:CDS 35.0%
CATAACCAAACTGTTTGACT+TGG + tig0032224:13543-13562 None:intergenic 35.0%
GTCAAACAGTTTGGTTATGA+AGG - tig0032224:13544-13563 Msa1448580:CDS 35.0%
!! ACTCTTAGTTTTCCTGAAAG+TGG - tig0032224:13577-13596 Msa1448580:CDS 35.0%
AGAGTTTGGCAAAATCATAG+CGG - tig0032224:13603-13622 Msa1448580:CDS 35.0%
TCCAATCACACAGAAAGAAA+TGG - tig0032224:13832-13851 Msa1448580:CDS 35.0%
TCCATTTCTTTCTGTGTGAT+TGG + tig0032224:13836-13855 None:intergenic 35.0%
CATATTCTGGATACTTTGCT+AGG + tig0032224:13877-13896 None:intergenic 35.0%
GCAAATCTAATTGAAGGAGA+TGG - tig0032224:13896-13915 Msa1448580:CDS 35.0%
AGAACCAAATACTCATCATC+GGG + tig0032224:14166-14185 None:intergenic 35.0%
! TCTTTTCACTCCAAGTTACA+AGG - tig0032224:14183-14202 Msa1448580:intron 35.0%
ATCATAGCATCCTTGTAACT+TGG + tig0032224:14196-14215 None:intergenic 35.0%
TTACAAGGATGCTATGATGT+TGG - tig0032224:14198-14217 Msa1448580:intron 35.0%
TATTCTTGGTGAACAACAAG+AGG - tig0032224:14264-14283 Msa1448580:CDS 35.0%
! ACATAAGCAAATAGCCCTTT+TGG + tig0032224:14415-14434 None:intergenic 35.0%
CTTATGTAGCAGATGTGAAT+GGG - tig0032224:14428-14447 Msa1448580:intron 35.0%
!! ACAAGTGATTTTTGAAGGGT+TGG + tig0032224:14596-14615 None:intergenic 35.0%
CATTCATCCATTAACCTAAG+AGG + tig0032224:14659-14678 None:intergenic 35.0%
TAAACCATGGTTAAGTCCAT+AGG - tig0032224:13123-13142 Msa1448580:three_prime_UTR 35.0%
AATTTGTGTATAGGTCCAAC+AGG + tig0032224:13506-13525 None:intergenic 35.0%
GAATACCAAGTCAAACAGTT+TGG - tig0032224:13535-13554 Msa1448580:CDS 35.0%
CATAACCAAACTGTTTGACT+TGG + tig0032224:13543-13562 None:intergenic 35.0%
GTCAAACAGTTTGGTTATGA+AGG - tig0032224:13544-13563 Msa1448580:CDS 35.0%
!! ACTCTTAGTTTTCCTGAAAG+TGG - tig0032224:13577-13596 Msa1448580:CDS 35.0%
AGAGTTTGGCAAAATCATAG+CGG - tig0032224:13603-13622 Msa1448580:CDS 35.0%
TCCAATCACACAGAAAGAAA+TGG - tig0032224:13832-13851 Msa1448580:CDS 35.0%
TCCATTTCTTTCTGTGTGAT+TGG + tig0032224:13836-13855 None:intergenic 35.0%
CATATTCTGGATACTTTGCT+AGG + tig0032224:13877-13896 None:intergenic 35.0%
GCAAATCTAATTGAAGGAGA+TGG - tig0032224:13896-13915 Msa1448580:CDS 35.0%
AGAACCAAATACTCATCATC+GGG + tig0032224:14166-14185 None:intergenic 35.0%
! TCTTTTCACTCCAAGTTACA+AGG - tig0032224:14183-14202 Msa1448580:intron 35.0%
ATCATAGCATCCTTGTAACT+TGG + tig0032224:14196-14215 None:intergenic 35.0%
TTACAAGGATGCTATGATGT+TGG - tig0032224:14198-14217 Msa1448580:intron 35.0%
TATTCTTGGTGAACAACAAG+AGG - tig0032224:14264-14283 Msa1448580:CDS 35.0%
! ACATAAGCAAATAGCCCTTT+TGG + tig0032224:14415-14434 None:intergenic 35.0%
CTTATGTAGCAGATGTGAAT+GGG - tig0032224:14428-14447 Msa1448580:intron 35.0%
!! ACAAGTGATTTTTGAAGGGT+TGG + tig0032224:14596-14615 None:intergenic 35.0%
CATTCATCCATTAACCTAAG+AGG + tig0032224:14659-14678 None:intergenic 35.0%
AGATCCTATGGACTTAACCA+TGG + tig0032224:13130-13149 None:intergenic 40.0%
TCCAACAGGAACTTTCTTAG+TGG + tig0032224:13492-13511 None:intergenic 40.0%
CCAAACTCTATACCACTTTC+AGG + tig0032224:13592-13611 None:intergenic 40.0%
CCTGAAAGTGGTATAGAGTT+TGG - tig0032224:13589-13608 Msa1448580:CDS 40.0%
TGTTTGGATCGACAGATAAG+TGG + tig0032224:13668-13687 None:intergenic 40.0%
ACCATCTTAGGTCAATGTCT+CGG + tig0032224:13808-13827 None:intergenic 40.0%
CTAATTGAAGGAGATGGAAG+TGG - tig0032224:13902-13921 Msa1448580:CDS 40.0%
CATTATCAATGAGGAGCCTT+CGG - tig0032224:13943-13962 Msa1448580:CDS 40.0%
! TCTGACATTGGTAATTCCGA+AGG + tig0032224:13962-13981 None:intergenic 40.0%
AACTGTGTCCTATCCATTTC+AGG + tig0032224:14031-14050 None:intergenic 40.0%
TTTGCCCGATGATGAGTATT+TGG - tig0032224:14159-14178 Msa1448580:intron 40.0%
AAGGATGCTATGATGTTGGT+GGG - tig0032224:14202-14221 Msa1448580:intron 40.0%
TTCTCATAGCACCTAAGACA+TGG - tig0032224:14326-14345 Msa1448580:CDS 40.0%
CTCAAATCAAGCCATGTCTT+AGG + tig0032224:14340-14359 None:intergenic 40.0%
! CTAAGACATGGCTTGATTTG+AGG - tig0032224:14338-14357 Msa1448580:intron 40.0%
! TGGCTTGATTTGAGGATTAG+AGG - tig0032224:14346-14365 Msa1448580:intron 40.0%
! GGCTTGATTTGAGGATTAGA+GGG - tig0032224:14347-14366 Msa1448580:intron 40.0%
GCTTATGTAGCAGATGTGAA+TGG - tig0032224:14427-14446 Msa1448580:intron 40.0%
ATGTGAATGGGACAATGCAT+TGG - tig0032224:14440-14459 Msa1448580:intron 40.0%
TGTGAATGGGACAATGCATT+GGG - tig0032224:14441-14460 Msa1448580:intron 40.0%
TTTGCTTGATGCATCTGCAA+TGG - tig0032224:14495-14514 Msa1448580:exon 40.0%
AACGGTTCCTCTTAGGTTAA+TGG - tig0032224:14649-14668 Msa1448580:five_prime_UTR 40.0%
AGATCCTATGGACTTAACCA+TGG + tig0032224:13130-13149 None:intergenic 40.0%
TCCAACAGGAACTTTCTTAG+TGG + tig0032224:13492-13511 None:intergenic 40.0%
CCAAACTCTATACCACTTTC+AGG + tig0032224:13592-13611 None:intergenic 40.0%
CCTGAAAGTGGTATAGAGTT+TGG - tig0032224:13589-13608 Msa1448580:CDS 40.0%
TGTTTGGATCGACAGATAAG+TGG + tig0032224:13668-13687 None:intergenic 40.0%
ACCATCTTAGGTCAATGTCT+CGG + tig0032224:13808-13827 None:intergenic 40.0%
CTAATTGAAGGAGATGGAAG+TGG - tig0032224:13902-13921 Msa1448580:CDS 40.0%
CATTATCAATGAGGAGCCTT+CGG - tig0032224:13943-13962 Msa1448580:CDS 40.0%
! TCTGACATTGGTAATTCCGA+AGG + tig0032224:13962-13981 None:intergenic 40.0%
AACTGTGTCCTATCCATTTC+AGG + tig0032224:14031-14050 None:intergenic 40.0%
TTTGCCCGATGATGAGTATT+TGG - tig0032224:14159-14178 Msa1448580:intron 40.0%
AAGGATGCTATGATGTTGGT+GGG - tig0032224:14202-14221 Msa1448580:intron 40.0%
TTCTCATAGCACCTAAGACA+TGG - tig0032224:14326-14345 Msa1448580:CDS 40.0%
CTCAAATCAAGCCATGTCTT+AGG + tig0032224:14340-14359 None:intergenic 40.0%
! CTAAGACATGGCTTGATTTG+AGG - tig0032224:14338-14357 Msa1448580:intron 40.0%
! TGGCTTGATTTGAGGATTAG+AGG - tig0032224:14346-14365 Msa1448580:intron 40.0%
! GGCTTGATTTGAGGATTAGA+GGG - tig0032224:14347-14366 Msa1448580:intron 40.0%
GCTTATGTAGCAGATGTGAA+TGG - tig0032224:14427-14446 Msa1448580:intron 40.0%
ATGTGAATGGGACAATGCAT+TGG - tig0032224:14440-14459 Msa1448580:intron 40.0%
TGTGAATGGGACAATGCATT+GGG - tig0032224:14441-14460 Msa1448580:intron 40.0%
TTTGCTTGATGCATCTGCAA+TGG - tig0032224:14495-14514 Msa1448580:exon 40.0%
AACGGTTCCTCTTAGGTTAA+TGG - tig0032224:14649-14668 Msa1448580:five_prime_UTR 40.0%
TACCATGGGAACAAACAGAG+AGG - tig0032224:13294-13313 Msa1448580:CDS 45.0%
ACCATGGGAACAAACAGAGA+GGG - tig0032224:13295-13314 Msa1448580:CDS 45.0%
GCCACTAAGAAAGTTCCTGT+TGG - tig0032224:13488-13507 Msa1448580:CDS 45.0%
TGAGCAGGAATCACCACATT+TGG + tig0032224:13758-13777 None:intergenic 45.0%
GCTTCAGCTATGATATGAGC+AGG + tig0032224:13773-13792 None:intergenic 45.0%
TCCGAGACATTGACCTAAGA+TGG - tig0032224:13804-13823 Msa1448580:CDS 45.0%
GACATTGACCTAAGATGGTC+AGG - tig0032224:13809-13828 Msa1448580:CDS 45.0%
TGATTGGACCTGACCATCTT+AGG + tig0032224:13820-13839 None:intergenic 45.0%
CTTCCAAAAGAAGGCGATCT+TGG + tig0032224:14001-14020 None:intergenic 45.0%
GGAAGGTTGCTTCCAAAAGA+AGG + tig0032224:14010-14029 None:intergenic 45.0%
TGGAAGCAACCTTCCTGAAA+TGG - tig0032224:14015-14034 Msa1448580:CDS 45.0%
GCAACCTTCCTGAAATGGAT+AGG - tig0032224:14020-14039 Msa1448580:CDS 45.0%
GTGTCCTATCCATTTCAGGA+AGG + tig0032224:14027-14046 None:intergenic 45.0%
! GGTATCGAGATGAAACTTCC+CGG + tig0032224:14100-14119 None:intergenic 45.0%
CAAGGATGCTATGATGTTGG+TGG - tig0032224:14201-14220 Msa1448580:intron 45.0%
GACAATGCATTGGGTTTCTG+AGG - tig0032224:14450-14469 Msa1448580:intron 45.0%
!! ATTGGGTTTCTGAGGCTCAT+AGG - tig0032224:14458-14477 Msa1448580:intron 45.0%
CTGAGGCTCATAGGAACTAT+TGG - tig0032224:14467-14486 Msa1448580:intron 45.0%
TGATGCATCTGCAATGGTTG+TGG - tig0032224:14501-14520 Msa1448580:exon 45.0%
GATGCATCTGCAATGGTTGT+GGG - tig0032224:14502-14521 Msa1448580:exon 45.0%
ATCTGCAATGGTTGTGGGAA+AGG - tig0032224:14507-14526 Msa1448580:exon 45.0%
CAATGGTTGTGGGAAAGGAT+AGG - tig0032224:14512-14531 Msa1448580:five_prime_UTR 45.0%
GGAAAGGATAGGCTACATGT+TGG - tig0032224:14523-14542 Msa1448580:five_prime_UTR 45.0%
GGCTACATGTTGGACTTCAT+AGG - tig0032224:14533-14552 Msa1448580:five_prime_UTR 45.0%
AACCTAAGAGGAACCGTTTG+AGG + tig0032224:14647-14666 None:intergenic 45.0%
TACCATGGGAACAAACAGAG+AGG - tig0032224:13294-13313 Msa1448580:CDS 45.0%
ACCATGGGAACAAACAGAGA+GGG - tig0032224:13295-13314 Msa1448580:CDS 45.0%
GCCACTAAGAAAGTTCCTGT+TGG - tig0032224:13488-13507 Msa1448580:CDS 45.0%
TGAGCAGGAATCACCACATT+TGG + tig0032224:13758-13777 None:intergenic 45.0%
GCTTCAGCTATGATATGAGC+AGG + tig0032224:13773-13792 None:intergenic 45.0%
TCCGAGACATTGACCTAAGA+TGG - tig0032224:13804-13823 Msa1448580:CDS 45.0%
GACATTGACCTAAGATGGTC+AGG - tig0032224:13809-13828 Msa1448580:CDS 45.0%
TGATTGGACCTGACCATCTT+AGG + tig0032224:13820-13839 None:intergenic 45.0%
CTTCCAAAAGAAGGCGATCT+TGG + tig0032224:14001-14020 None:intergenic 45.0%
GGAAGGTTGCTTCCAAAAGA+AGG + tig0032224:14010-14029 None:intergenic 45.0%
TGGAAGCAACCTTCCTGAAA+TGG - tig0032224:14015-14034 Msa1448580:CDS 45.0%
GCAACCTTCCTGAAATGGAT+AGG - tig0032224:14020-14039 Msa1448580:CDS 45.0%
GTGTCCTATCCATTTCAGGA+AGG + tig0032224:14027-14046 None:intergenic 45.0%
! GGTATCGAGATGAAACTTCC+CGG + tig0032224:14100-14119 None:intergenic 45.0%
CAAGGATGCTATGATGTTGG+TGG - tig0032224:14201-14220 Msa1448580:intron 45.0%
GACAATGCATTGGGTTTCTG+AGG - tig0032224:14450-14469 Msa1448580:intron 45.0%
!! ATTGGGTTTCTGAGGCTCAT+AGG - tig0032224:14458-14477 Msa1448580:intron 45.0%
CTGAGGCTCATAGGAACTAT+TGG - tig0032224:14467-14486 Msa1448580:intron 45.0%
TGATGCATCTGCAATGGTTG+TGG - tig0032224:14501-14520 Msa1448580:exon 45.0%
GATGCATCTGCAATGGTTGT+GGG - tig0032224:14502-14521 Msa1448580:exon 45.0%
ATCTGCAATGGTTGTGGGAA+AGG - tig0032224:14507-14526 Msa1448580:exon 45.0%
CAATGGTTGTGGGAAAGGAT+AGG - tig0032224:14512-14531 Msa1448580:five_prime_UTR 45.0%
GGAAAGGATAGGCTACATGT+TGG - tig0032224:14523-14542 Msa1448580:five_prime_UTR 45.0%
GGCTACATGTTGGACTTCAT+AGG - tig0032224:14533-14552 Msa1448580:five_prime_UTR 45.0%
AACCTAAGAGGAACCGTTTG+AGG + tig0032224:14647-14666 None:intergenic 45.0%
ACCCTCTCTGTTTGTTCCCA+TGG + tig0032224:13299-13318 None:intergenic 50.0%
GGAACAAACAGAGAGGGTCT+TGG - tig0032224:13301-13320 Msa1448580:CDS 50.0%
ACAAACAGAGAGGGTCTTGG+AGG - tig0032224:13304-13323 Msa1448580:CDS 50.0%
! GTCTTGGAGGATCTGAGGTA+AGG - tig0032224:13317-13336 Msa1448580:CDS 50.0%
! TCGCCAAGATCGCCTTCTTT+TGG - tig0032224:13995-14014 Msa1448580:CDS 50.0%
CGAGATGAAACTTCCCGGAA+AGG + tig0032224:14095-14114 None:intergenic 50.0%
CACCTCAAACGGTTCCTCTT+AGG - tig0032224:14642-14661 Msa1448580:five_prime_UTR 50.0%
ACCCTCTCTGTTTGTTCCCA+TGG + tig0032224:13299-13318 None:intergenic 50.0%
GGAACAAACAGAGAGGGTCT+TGG - tig0032224:13301-13320 Msa1448580:CDS 50.0%
ACAAACAGAGAGGGTCTTGG+AGG - tig0032224:13304-13323 Msa1448580:CDS 50.0%
! GTCTTGGAGGATCTGAGGTA+AGG - tig0032224:13317-13336 Msa1448580:CDS 50.0%
! TCGCCAAGATCGCCTTCTTT+TGG - tig0032224:13995-14014 Msa1448580:CDS 50.0%
CGAGATGAAACTTCCCGGAA+AGG + tig0032224:14095-14114 None:intergenic 50.0%
CACCTCAAACGGTTCCTCTT+AGG - tig0032224:14642-14661 Msa1448580:five_prime_UTR 50.0%
AGAGGGTCTTGGAGGATCTG+AGG - tig0032224:13312-13331 Msa1448580:CDS 55.0%
AGAGGGTCTTGGAGGATCTG+AGG - tig0032224:13312-13331 Msa1448580:CDS 55.0%
Chromosome Type Strat End Strand Name
tig0032224 gene 13064 14742 13064 ID=Msa1448580;Name=Msa1448580
tig0032224 mRNA 13064 14742 13064 ID=Msa1448580-mRNA-1;Parent=Msa1448580;Name=Msa1448580-mRNA-1;_AED=0.01;_eAED=0.01;_QI=234|1|1|1|1|1|3|79|353
tig0032224 exon 13064 14054 13064 ID=Msa1448580-mRNA-1:exon:16483;Parent=Msa1448580-mRNA-1
tig0032224 exon 14241 14354 14241 ID=Msa1448580-mRNA-1:exon:16482;Parent=Msa1448580-mRNA-1
tig0032224 exon 14473 14742 14473 ID=Msa1448580-mRNA-1:exon:16481;Parent=Msa1448580-mRNA-1
tig0032224 five_prime_UTR 14509 14742 14509 ID=Msa1448580-mRNA-1:five_prime_utr;Parent=Msa1448580-mRNA-1
tig0032224 CDS 14473 14508 14473 ID=Msa1448580-mRNA-1:cds;Parent=Msa1448580-mRNA-1
tig0032224 CDS 14241 14354 14241 ID=Msa1448580-mRNA-1:cds;Parent=Msa1448580-mRNA-1
tig0032224 CDS 13143 14054 13143 ID=Msa1448580-mRNA-1:cds;Parent=Msa1448580-mRNA-1
tig0032224 three_prime_UTR 13064 13142 13064 ID=Msa1448580-mRNA-1:three_prime_utr;Parent=Msa1448580-mRNA-1
Gene Sequence

>Msa1448580

ATGGGAACAAACAGAGAGGGTCTTGGAGGATCTGAGGCAATATATCATGAAATGGTAGAAGATGAAGTTAAGGCCACTAAGAAAGTTCCTGTTGGACCTATACACAAATTAATAGCAGAGAATACCAAGTCAAACAGTTTGGTTATGAAGAAACCAAATGTGGTGATTCCTGCTCATATCATAGCTGAAGCAATATCAACTATCCGAGACATTGACCTAAGATGGTCAGGTCCAATCACACAGAAAGAAATGGAATATGTAGAAGAATATGTCCTAGCAAAGTATCCAGAATATGCAAATCTAATTGAAGGAGATGGAAGTGGAATAGACATGTCAAGTTTCATTATCAATGAGGAGCCTTCGGAATTACCAATGTCAGATGATAGAAGAAAGTCGCCAAGATCGCCTTCTTTTGGAAGCAACCTTCCTGAAATGGATAGGACACAGTTAGAGCCATCAAGACTACTTGATATTCTCAACAAAAAATCATCCTTTCCGGGAAGTTTCATCTCGATACCAGAAATTCAAGCTCAAAATAAAGTTTTGAAGCATTGTGGTTTGCCCGATGATGAGTATTTGGTTCTTTTCACTCCAAGTTACAAGGATGCTATGATGTTGGTGGGAGAAAGTTATCCTTTTGTTAAGGGAAACTACTACATGACTATTCTTGGTGAACAACAAGAGGACTATATCAAAGAATTTGCTTCTTTTAAGGAATCAAAGGTTCTCATAGCACCTAAGACATGGCTTGATTTGAGGATTAGAGGGTCTCAGTTAAGTCAAAATTTTAGGAGGAAATGTAAGATTAGTCCAAAAGGGCTATTTGCTTATGTAGCAGATGTGAATGGGACAATGCATTGGGTTTCTGAGGCTCATAGGAACTATTGGCATGTTTTGCTTGATGCATCTGCAATGGTTGTGGGAAAGGATAGGCTACATGTTGGACTTCATAGGCCTGATTTCTTAGTTTGTTGTCTTGATAACACAAATTCCAACCCTTCAAAAATCACTTGTCTCTTGGTTAGAAAGAAAAGTTTTGACACCTCAAACGGTTCCTCTTAG
Protein sequence

>Msa1448580

MGTNREGLGGSEAIYHEMVEDEVKATKKVPVGPIHKLIAENTKSNSLVMKKPNVVIPAHIIAEAISTIRDIDLRWSGPITQKEMEYVEEYVLAKYPEYANLIEGDGSGIDMSSFIINEEPSELPMSDDRRKSPRSPSFGSNLPEMDRTQLEPSRLLDILNKKSSFPGSFISIPEIQAQNKVLKHCGLPDDEYLVLFTPSYKDAMMLVGESYPFVKGNYYMTILGEQQEDYIKEFASFKESKVLIAPKTWLDLRIRGSQLSQNFRRKCKISPKGLFAYVADVNGTMHWVSEAHRNYWHVLLDASAMVVGKDRLHVGLHRPDFLVCCLDNTNSNPSKITCLLVRKKSFDTSNGSS*