AlfalfaGEDB | Msa1449060

M. sativa cultivar zhongmu-4 / Msa1449060

KEGG: -
GO: GO:0005575 GO:0016020
PFAM: PF01453.27

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1449060	sp\|P17801\|KPRO_MAIZE	39.802	809	435	19	15	786	19	812	0.0	568

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1449060	G7L1A0	95.712	793	34	0	1	793	1	793	0.0	1563

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0342550	Msa1449060	0.937287	4.425846e-98	-8.615850e-47
Msa1148660	Msa1449060	0.987361	5.559102e-170	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa1449060	MtrunA17_Chr7g0237341	95.712	793	34	0	1	793	1	793	1563
Msa1449060	MtrunA17_Chr7g0237331	82.893	795	133	3	1	793	1	794	1339
Msa1449060	MtrunA17_Chr7g0237351	67.168	798	249	10	6	792	1	796	1055
Msa1449060	MtrunA17_Chr4g0053161	67.621	803	248	10	1	794	18	817	1044
Msa1449060	MtrunA17_Chr7g0237391	68.164	779	231	9	20	787	18	790	1004
Msa1449060	MtrunA17_Chr7g0234561	68.616	701	211	7	93	787	34	731	971
Msa1449060	MtrunA17_Chr3g0106201	59.669	786	298	10	12	786	10	787	901
Msa1449060	MtrunA17_Chr3g0106211	57.340	797	313	11	8	786	11	798	887
Msa1449060	MtrunA17_Chr3g0106121	57.373	807	318	12	4	792	1	799	884
Msa1449060	MtrunA17_Chr3g0106221	58.780	803	298	13	10	786	8	803	884

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1449060	AT2G19130.1	32.103	813	467	24	11	787	4	767	1.84e-115	369
Msa1449060	AT5G24080.2	33.373	851	434	32	8	787	14	802	3.89e-108	351
Msa1449060	AT4G00340.1	31.258	771	463	25	42	787	32	760	2.33e-97	321
Msa1449060	AT4G00340.2	30.378	767	454	23	42	787	32	739	1.58e-94	313
Msa1449060	AT1G34300.1	30.730	794	461	28	24	787	23	757	5.93e-94	312
Msa1449060	AT5G24080.3	33.516	728	361	27	130	787	1	675	8.16e-93	307
Msa1449060	AT5G24080.1	33.516	728	361	27	130	787	1	675	8.16e-93	307
Msa1449060	AT5G35370.1	30.477	817	434	33	43	787	50	804	2.87e-89	300
Msa1449060	AT4G32300.1	29.161	775	458	27	45	787	50	765	1.37e-83	284
Msa1449060	AT4G11900.1	29.229	869	456	37	8	786	9	808	5.49e-77	266

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Find 167 sgRNAs with CRISPR-Local

Find 378 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCTTGGAAGCTAATTT+CGG	0.364151	tig0032425:-38900	Msa1449060:CDS
AAACACAATTGCGAAATGGA+AGG	0.613290	tig0032425:-38337	Msa1449060:CDS
AAAGAGGCAGTAACATTTCT+TGG	0.270952	tig0032425:-37329	Msa1449060:CDS
AAAGGATTTCAGTACGGTTT+CGG	0.309229	tig0032425:-38392	Msa1449060:CDS
AAAGGGTTCAGTCAAGAGAT+TGG	0.465609	tig0032425:-37993	Msa1449060:CDS
AAAGGGTTCCTCCCTCTCAG+TGG	0.652420	tig0032425:-39440	Msa1449060:CDS
AAATTATGATATAAGTAAAA+TGG	0.329221	tig0032425:-37274	Msa1449060:CDS
AACATTTACCATCTCGAAAA+AGG	0.291488	tig0032425:+38499	None:intergenic
AACCATAAGACCAATCGCTA+TGG	0.597360	tig0032425:+38564	None:intergenic
AACCATAGCGATTGGTCTTA+TGG	0.284619	tig0032425:-38566	Msa1449060:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CACACTCTTTATTCTAGTAT+TGG	-	tig0032425:37187-37206	Msa1449060:CDS	30.0%
TTCTTCTTCATCATTGAGAA+AGG	-	tig0032425:37232-37251	Msa1449060:CDS	30.0%
TCTTCTTCATCATTGAGAAA+GGG	-	tig0032425:37233-37252	Msa1449060:CDS	30.0%
AGTGTTTAAAAGGGTAAGTT+TGG	+	tig0032425:37450-37469	None:intergenic	30.0%
CAATAAGAGCTAGAAATATC+AGG	+	tig0032425:37767-37786	None:intergenic	30.0%
AAAACCTTGGAAGCTAATTT+CGG	-	tig0032425:37790-37809	Msa1449060:CDS	30.0%
TGGATTCTGATGGTAATTTA+AGG	-	tig0032425:37942-37961	Msa1449060:CDS	30.0%
GGATTCTGATGGTAATTTAA+GGG	-	tig0032425:37943-37962	Msa1449060:CDS	30.0%
TGTAGTTATAATCCTAAGCA+TGG	-	tig0032425:38064-38083	Msa1449060:CDS	30.0%
AGAAAATGTTCTTGTATTCC+GGG	-	tig0032425:38088-38107	Msa1449060:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0032425	gene	37164	39548	37164	ID=Msa1449060;Name=Msa1449060
tig0032425	mRNA	37164	39548	37164	ID=Msa1449060-mRNA-1;Parent=Msa1449060;Name=Msa1449060-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|794
tig0032425	exon	37164	39548	37164	ID=Msa1449060-mRNA-1:exon:16754;Parent=Msa1449060-mRNA-1
tig0032425	CDS	37164	39548	37164	ID=Msa1449060-mRNA-1:cds;Parent=Msa1449060-mRNA-1

Gene Sequence

>Msa1449060

ATGAATAATATGGGTTTGTCATTCACACTCTTTATTCTAGTATTGGCCTTTTTATTTCAATGTTCATCTTCTTCTTCATCATTGAGAAAGGGTTCCTCCCTCTCAGTGGAAAATCCAGAAGACAACATCATCTCACAAAATGGCATGTTCTCTGCTGGCTTTACTGCTATTGGTGACAATGCATATTCCTTTGCCATATGGTTCATAGAACCAAATTCCCTCAACCTCAACAACACCATTATTTGGATGGCAAATCGCGACCAACCCGTGAATGGAAAACGATCCAAACTTACCCTTTTAAACACTGGAAATATTGTATTGTTTGATGTTTCATTAAACAATGTTTGGTCTTCAAACACTGCATCATTGAAACCATTAGAGTTGCATCTCAAAAATGATGGAAATCTTGTGTTGCGTGAGCTTCAAGGAATCACAATTTTGTGGCAAAGTTTTGACTCTCCAACAGATACTCTCCTTCCAGGTCAACCTCTTACAAGATATACAAAGCTTGTAGCTTCAAGAAGTGAGAGTAATCATTCTACTGGCTTCTACAAGTTCTACTTTGATGATGCAAATGTTCTTGGTCTTCATTACGATGGCCCTGATATTTCTAGCTCTTATTGGCCAAAACCTTGGAAGCTAATTTCGGAAATTGGTAGGGCTAATTTCAATGGTAGCAGAATTGCATTGTTGGATTCTTTTGGTAGTTTCCTTTCATCAGATAATTTGACTTTTATAACTTCTGATTATGGAACAGTTCTACAAAGAAGAATGAAAATGGATTCTGATGGTAATTTAAGGGTGTATAGTAGAATTAATGTGTCACAAAATTGGTACGTTTCATGGCAAGCCATTTATGGTGGTTGTATAGCTCATGGGATTTGTGGAGATAATAGCACATGTAGTTATAATCCTAAGCATGGAAGAAAATGTTCTTGTATTCCGGGATATAAAGTGAAGAACCATAGCGATTGGTCTTATGGTTGCGAGCCTATGTTTGATTTTACTTGCAATAGAAGTGAGTCTACCTTTTTCGAGATGGTAAATGTTGAGTTTTATGGCTATGACATTCATTATGTTCCTATGAGTAACTATAGTTCTTGTGAGAAATTGTGCCTAGGAGATTGTAATTGTAAAGGATTTCAGTACGGTTTCGGGGTACTAAAAGGATTTTATAAGTGTTATACAAAAACACAATTGCGAAATGGAAGGCATTCACCATTCTTTGTAGGAACAACCTACTTGAGGTTGCCTAAAGGTAATAACTTCTCTAAAGAAGAATCTTCCACTCCAAGTGACCATGTTTGTCTTGTGAAACTTCAAAGAAATTTTGTTAGACCAAGTGAGAACCATTATGTGAATTTTTTTCTATGGTTTGCCGCTGCAATCGGAGCTTTTGAAGCGGTCTGCATTTTCATCGTTTGGTGTTCCTTATTCAGGAACCGAAAGACAAATGCAGACCAACACGGATACCACCAACTCGCAGCAATAGGGTTTAGAAAATTTAGTTACTTGGAGTTAAAGAAAGCAACAAAAGGGTTCAGTCAAGAGATTGGAAGAGGTGGAGGAGGTATCGTGTACAAAGGACTATTATCTGATCAAAGACATGCTGCAATAAAAAGACTTTACAATGCTCAACAAGGAGAAGGTGAATTTCTAGCTGAAGTAGGAATCATTGGAAGGCTTAATCACATGAATTTGATTGAAATGTGGGGATATTGTGCCGAGGGAAAATATAGACTATTGGTTTATGAGTACATGGAAAACGGTTCTTTAGCCGAAAACTTGTTGGCTAATAAACTTGATTGGAGTAAAAGGTACAAAATTGCTTTAGCAATAGCAAGAGTTTTAGCATATCTACATGAAGAATGTTTGGAGTGGATTTTACATTGTGATATAAAGCCACAAAACATACTTCTTGATTCTAACTTCCAGCCCAAGTTAGCTGATTTTGGATTGTCTAAGCTGCAAAACAGGAACAACCTTAATAATTCATGCATCTCAATGATTAGAGGAACAAGAGGATATATGGCACCTGAATGGATTTTCAATTTGCCAATAACATCTAAGGTGGATGTTTATAGTTATGGAATAGTTGTGTTGGAAATGATAACAGGAAAAAGTCCAACTACAGGTTTCAAAATTGTAAATGGAGAAGAGGAAAGTGATGGGAGGTTGGTTACATGGGTGAGAGAAAAAAGAGGCAGTAACATTTCTTGGTTAGAGGAAATTGTTGACCCTCAAATTGGATTAAATTATGATATAAGTAAAATGGAGATTTTGGCTAAAGTTGCTTTGGATTGTGTTGTGGATGAAAGAGATTCAAGGCCTACCATGAGTAAAGTAGTTGAGATGCTTCAATACCATGGAAGTGATGTTGAGTGA

Protein sequence

>Msa1449060

MNNMGLSFTLFILVLAFLFQCSSSSSSLRKGSSLSVENPEDNIISQNGMFSAGFTAIGDNAYSFAIWFIEPNSLNLNNTIIWMANRDQPVNGKRSKLTLLNTGNIVLFDVSLNNVWSSNTASLKPLELHLKNDGNLVLRELQGITILWQSFDSPTDTLLPGQPLTRYTKLVASRSESNHSTGFYKFYFDDANVLGLHYDGPDISSSYWPKPWKLISEIGRANFNGSRIALLDSFGSFLSSDNLTFITSDYGTVLQRRMKMDSDGNLRVYSRINVSQNWYVSWQAIYGGCIAHGICGDNSTCSYNPKHGRKCSCIPGYKVKNHSDWSYGCEPMFDFTCNRSESTFFEMVNVEFYGYDIHYVPMSNYSSCEKLCLGDCNCKGFQYGFGVLKGFYKCYTKTQLRNGRHSPFFVGTTYLRLPKGNNFSKEESSTPSDHVCLVKLQRNFVRPSENHYVNFFLWFAAAIGAFEAVCIFIVWCSLFRNRKTNADQHGYHQLAAIGFRKFSYLELKKATKGFSQEIGRGGGGIVYKGLLSDQRHAAIKRLYNAQQGEGEFLAEVGIIGRLNHMNLIEMWGYCAEGKYRLLVYEYMENGSLAENLLANKLDWSKRYKIALAIARVLAYLHEECLEWILHCDIKPQNILLDSNFQPKLADFGLSKLQNRNNLNNSCISMIRGTRGYMAPEWIFNLPITSKVDVYSYGIVVLEMITGKSPTTGFKIVNGEEESDGRLVTWVREKRGSNISWLEEIVDPQIGLNYDISKMEILAKVALDCVVDERDSRPTMSKVVEMLQYHGSDVE*