Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1449630 | A0A072UVF3 | 93.220 | 177 | 11 | 1 | 1 | 177 | 1 | 176 | 4.81e-106 | 310 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0095660 | Msa1449630 | 0.989739 | 1.960451e-179 | -8.615850e-47 |
| Msa1283800 | Msa1449630 | 0.803901 | 2.793785e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1449630 | MtrunA17_Chr3g0095001 | 93.220 | 177 | 11 | 1 | 1 | 177 | 1 | 176 | 9.27e-110 | 310 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 62 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATCCTCGCCAATCGTTTC+TGG | 0.178153 | tig0032661:+34812 | None:intergenic |
| GATCCGACGATCGGAGTTAC+TGG | 0.318047 | tig0032661:+34834 | None:intergenic |
| GCGCGTGGTGGTTTCGGAAT+TGG | 0.324550 | tig0032661:+35080 | None:intergenic |
| GTGAGAGCGCGTGGTGGTTT+CGG | 0.347030 | tig0032661:+35074 | None:intergenic |
| TCGGAATTGGATTCGGGTAA+TGG | 0.351220 | tig0032661:+35093 | None:intergenic |
| GGTGGTTTCGGAATTGGATT+CGG | 0.353642 | tig0032661:+35086 | None:intergenic |
| GTGAGAGAGTGGTAGTAAAA+AGG | 0.372256 | tig0032661:+35136 | None:intergenic |
| GTGGTTTCGGAATTGGATTC+GGG | 0.381893 | tig0032661:+35087 | None:intergenic |
| CGGAATTGGATTCGGGTAAT+GGG | 0.389896 | tig0032661:+35094 | None:intergenic |
| GGATTCACCGTGGTGGGGTC+AGG | 0.431073 | tig0032661:-34795 | Msa1449630:CDS |
| GGTGTCGTGTTGTTGGGTTT+TGG | 0.431727 | tig0032661:+35044 | None:intergenic |
| AATAGAAGTGACTCTGGTTA+TGG | 0.445343 | tig0032661:+35179 | None:intergenic |
| GGGTGGATGTGACGGTTGAT+TGG | 0.460462 | tig0032661:+34964 | None:intergenic |
| AGGAAACGGTGTCGTGTTGT+TGG | 0.468497 | tig0032661:+35037 | None:intergenic |
| ACCGTGGTGGGGTCAGGTAC+CGG | 0.479745 | tig0032661:-34789 | Msa1449630:CDS |
| GGAAACGGTGTCGTGTTGTT+GGG | 0.481656 | tig0032661:+35038 | None:intergenic |
| AAGATCATGTCGTTGCCGTA+AGG | 0.485439 | tig0032661:+34868 | None:intergenic |
| TGGCGAGGATTCACCGTGGT+GGG | 0.487660 | tig0032661:-34801 | Msa1449630:CDS |
| TGGGGTCAGGTACCGGCCGA+TGG | 0.490608 | tig0032661:-34782 | Msa1449630:CDS |
| CGGCAACGGCGGTGAGATAA+CGG | 0.496678 | tig0032661:+34913 | None:intergenic |
| TCTCACCGCCGTTGCCGTCA+TGG | 0.506128 | tig0032661:-34907 | Msa1449630:CDS |
| TTTCATTCGCCGCGATGAGA+AGG | 0.508643 | tig0032661:-34743 | Msa1449630:CDS |
| GTCGATGGAGTTGAGATTGA+AGG | 0.511417 | tig0032661:+35017 | None:intergenic |
| AACGGCAATATGCGTTGGTG+TGG | 0.521157 | tig0032661:+34943 | None:intergenic |
| AAGGAAGAGTAAAACGGTGA+TGG | 0.539615 | tig0032661:+35155 | None:intergenic |
| CACCGCCGTCAGATCCCTTA+CGG | 0.546747 | tig0032661:-34883 | Msa1449630:CDS |
| TCGTTTCTGGATCCGACGAT+CGG | 0.553335 | tig0032661:+34825 | None:intergenic |
| AGTTAACGGAACGTGGTCGA+TGG | 0.554931 | tig0032661:+35002 | None:intergenic |
| AGTCTAACGGCAATATGCGT+TGG | 0.555667 | tig0032661:+34938 | None:intergenic |
| ATCATCATGATGAGAAGGGT+TGG | 0.556355 | tig0032661:-34711 | Msa1449630:CDS |
| ATCGCGGGCGCGTGAGAGAG+TGG | 0.558600 | tig0032661:+35125 | None:intergenic |
| GGATGTGACGGTTGATTGGA+CGG | 0.559632 | tig0032661:+34968 | None:intergenic |
| CGGAAATCGAGGAAAGTTAA+CGG | 0.560543 | tig0032661:+34988 | None:intergenic |
| GGAGTTGAGATTGAAGGAAA+CGG | 0.563289 | tig0032661:+35023 | None:intergenic |
| TGAGATAACGGAAAGTCTAA+CGG | 0.564277 | tig0032661:+34925 | None:intergenic |
| TCGTCATCATCATGATGAGA+AGG | 0.564544 | tig0032661:-34716 | Msa1449630:CDS |
| GGTTGATTGGACGGAAATCG+AGG | 0.564608 | tig0032661:+34977 | None:intergenic |
| GGCGAGGATTCACCGTGGTG+GGG | 0.570494 | tig0032661:-34800 | Msa1449630:CDS |
| GGCGGTGACCATGACGGCAA+CGG | 0.573371 | tig0032661:+34899 | None:intergenic |
| GATGGAAATAGAAGTGACTC+TGG | 0.580506 | tig0032661:+35173 | None:intergenic |
| GTTGGTGTGGGTGGATGTGA+CGG | 0.586166 | tig0032661:+34956 | None:intergenic |
| GCAATATGCGTTGGTGTGGG+TGG | 0.597606 | tig0032661:+34947 | None:intergenic |
| AGTGACTCTGGTTATGGCCA+TGG | 0.597966 | tig0032661:+35185 | None:intergenic |
| AGATCATGTCGTTGCCGTAA+GGG | 0.599013 | tig0032661:+34869 | None:intergenic |
| CGTCATCATCATGATGAGAA+GGG | 0.603183 | tig0032661:-34715 | Msa1449630:CDS |
| CGTTGCCGTAAGGGATCTGA+CGG | 0.605253 | tig0032661:+34878 | None:intergenic |
| ATGCCAGTAACTCCGATCGT+CGG | 0.606961 | tig0032661:-34837 | Msa1449630:CDS |
| CGAGGAAAGTTAACGGAACG+TGG | 0.607466 | tig0032661:+34995 | None:intergenic |
| TTGGCGAGGATTCACCGTGG+TGG | 0.615362 | tig0032661:-34802 | Msa1449630:CDS |
| TCTGACGGCGGTGACCATGA+CGG | 0.619366 | tig0032661:+34893 | None:intergenic |
| TCGTCGGATCCAGAAACGAT+TGG | 0.624574 | tig0032661:-34821 | Msa1449630:CDS |
| GGTGACCATGACGGCAACGG+CGG | 0.633427 | tig0032661:+34902 | None:intergenic |
| TCGTTATAAGTCATCACCAT+CGG | 0.635222 | tig0032661:+34766 | None:intergenic |
| TATAAGTCATCACCATCGGC+CGG | 0.644387 | tig0032661:+34770 | None:intergenic |
| GGAAGAACTGTGAGAGCGCG+TGG | 0.644400 | tig0032661:+35065 | None:intergenic |
| ACGGCAATATGCGTTGGTGT+GGG | 0.646745 | tig0032661:+34944 | None:intergenic |
| TGCCGTAAGGGATCTGACGG+CGG | 0.663675 | tig0032661:+34881 | None:intergenic |
| GCCGGTACCTGACCCCACCA+CGG | 0.667549 | tig0032661:+34788 | None:intergenic |
| GGATCCAGAAACGATTGGCG+AGG | 0.676844 | tig0032661:-34816 | Msa1449630:CDS |
| AGAACTGTGAGAGCGCGTGG+TGG | 0.748898 | tig0032661:+35068 | None:intergenic |
| GACGACGATCCTTCTCATCG+CGG | 0.765053 | tig0032661:+34734 | None:intergenic |
| CGATTGGCGAGGATTCACCG+TGG | 0.837550 | tig0032661:-34805 | Msa1449630:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGTAAAAAGGAAGAGTAAAA+CGG | + | tig0032661:34709-34728 | None:intergenic | 25.0% |
| AATAGAAGTGACTCTGGTTA+TGG | + | tig0032661:34679-34698 | None:intergenic | 35.0% | |
| !! | TGAGATAACGGAAAGTCTAA+CGG | + | tig0032661:34933-34952 | None:intergenic | 35.0% |
| TCGTTATAAGTCATCACCAT+CGG | + | tig0032661:35092-35111 | None:intergenic | 35.0% | |
| GATGGAAATAGAAGTGACTC+TGG | + | tig0032661:34685-34704 | None:intergenic | 40.0% | |
| AAGGAAGAGTAAAACGGTGA+TGG | + | tig0032661:34703-34722 | None:intergenic | 40.0% | |
| GTGAGAGAGTGGTAGTAAAA+AGG | + | tig0032661:34722-34741 | None:intergenic | 40.0% | |
| !!! | TAATGGGTTTTGAAGATCGC+GGG | + | tig0032661:34748-34767 | None:intergenic | 40.0% |
| !!! | GTAATGGGTTTTGAAGATCG+CGG | + | tig0032661:34749-34768 | None:intergenic | 40.0% |
| GGAGTTGAGATTGAAGGAAA+CGG | + | tig0032661:34835-34854 | None:intergenic | 40.0% | |
| CGGAAATCGAGGAAAGTTAA+CGG | + | tig0032661:34870-34889 | None:intergenic | 40.0% | |
| TCGTCATCATCATGATGAGA+AGG | - | tig0032661:35139-35158 | Msa1449630:CDS | 40.0% | |
| CGTCATCATCATGATGAGAA+GGG | - | tig0032661:35140-35159 | Msa1449630:CDS | 40.0% | |
| ATCATCATGATGAGAAGGGT+TGG | - | tig0032661:35144-35163 | Msa1449630:CDS | 40.0% | |
| CGGAATTGGATTCGGGTAAT+GGG | + | tig0032661:34764-34783 | None:intergenic | 45.0% | |
| TCGGAATTGGATTCGGGTAA+TGG | + | tig0032661:34765-34784 | None:intergenic | 45.0% | |
| GTGGTTTCGGAATTGGATTC+GGG | + | tig0032661:34771-34790 | None:intergenic | 45.0% | |
| GGTGGTTTCGGAATTGGATT+CGG | + | tig0032661:34772-34791 | None:intergenic | 45.0% | |
| GTCGATGGAGTTGAGATTGA+AGG | + | tig0032661:34841-34860 | None:intergenic | 45.0% | |
| AGTCTAACGGCAATATGCGT+TGG | + | tig0032661:34920-34939 | None:intergenic | 45.0% | |
| AGATCATGTCGTTGCCGTAA+GGG | + | tig0032661:34989-35008 | None:intergenic | 45.0% | |
| AAGATCATGTCGTTGCCGTA+AGG | + | tig0032661:34990-35009 | None:intergenic | 45.0% | |
| TATAAGTCATCACCATCGGC+CGG | + | tig0032661:35088-35107 | None:intergenic | 45.0% | |
| !! | GGTGTCGTGTTGTTGGGTTT+TGG | + | tig0032661:34814-34833 | None:intergenic | 50.0% |
| !! | GGAAACGGTGTCGTGTTGTT+GGG | + | tig0032661:34820-34839 | None:intergenic | 50.0% |
| !! | AGGAAACGGTGTCGTGTTGT+TGG | + | tig0032661:34821-34840 | None:intergenic | 50.0% |
| AGTTAACGGAACGTGGTCGA+TGG | + | tig0032661:34856-34875 | None:intergenic | 50.0% | |
| CGAGGAAAGTTAACGGAACG+TGG | + | tig0032661:34863-34882 | None:intergenic | 50.0% | |
| GGTTGATTGGACGGAAATCG+AGG | + | tig0032661:34881-34900 | None:intergenic | 50.0% | |
| GGATGTGACGGTTGATTGGA+CGG | + | tig0032661:34890-34909 | None:intergenic | 50.0% | |
| ! | ACGGCAATATGCGTTGGTGT+GGG | + | tig0032661:34914-34933 | None:intergenic | 50.0% |
| ! | AACGGCAATATGCGTTGGTG+TGG | + | tig0032661:34915-34934 | None:intergenic | 50.0% |
| ATGCCAGTAACTCCGATCGT+CGG | - | tig0032661:35018-35037 | Msa1449630:CDS | 50.0% | |
| ! | TCGTTTCTGGATCCGACGAT+CGG | + | tig0032661:35033-35052 | None:intergenic | 50.0% |
| TCGTCGGATCCAGAAACGAT+TGG | - | tig0032661:35034-35053 | Msa1449630:CDS | 50.0% | |
| GAATCCTCGCCAATCGTTTC+TGG | + | tig0032661:35046-35065 | None:intergenic | 50.0% | |
| TTTCATTCGCCGCGATGAGA+AGG | - | tig0032661:35112-35131 | Msa1449630:CDS | 50.0% | |
| GGGTGGATGTGACGGTTGAT+TGG | + | tig0032661:34894-34913 | None:intergenic | 55.0% | |
| GTTGGTGTGGGTGGATGTGA+CGG | + | tig0032661:34902-34921 | None:intergenic | 55.0% | |
| ! | GCAATATGCGTTGGTGTGGG+TGG | + | tig0032661:34911-34930 | None:intergenic | 55.0% |
| CGTTGCCGTAAGGGATCTGA+CGG | + | tig0032661:34980-34999 | None:intergenic | 55.0% | |
| GATCCGACGATCGGAGTTAC+TGG | + | tig0032661:35024-35043 | None:intergenic | 55.0% | |
| GGATCCAGAAACGATTGGCG+AGG | - | tig0032661:35039-35058 | Msa1449630:CDS | 55.0% | |
| GACGACGATCCTTCTCATCG+CGG | + | tig0032661:35124-35143 | None:intergenic | 55.0% | |
| GCGCGTGGTGGTTTCGGAAT+TGG | + | tig0032661:34778-34797 | None:intergenic | 60.0% | |
| ! | GTGAGAGCGCGTGGTGGTTT+CGG | + | tig0032661:34784-34803 | None:intergenic | 60.0% |
| ! | AGAACTGTGAGAGCGCGTGG+TGG | + | tig0032661:34790-34809 | None:intergenic | 60.0% |
| ! | GGAAGAACTGTGAGAGCGCG+TGG | + | tig0032661:34793-34812 | None:intergenic | 60.0% |
| CGGCAACGGCGGTGAGATAA+CGG | + | tig0032661:34945-34964 | None:intergenic | 60.0% | |
| TCTGACGGCGGTGACCATGA+CGG | + | tig0032661:34965-34984 | None:intergenic | 60.0% | |
| CACCGCCGTCAGATCCCTTA+CGG | - | tig0032661:34972-34991 | Msa1449630:CDS | 60.0% | |
| TGCCGTAAGGGATCTGACGG+CGG | + | tig0032661:34977-34996 | None:intergenic | 60.0% | |
| CGATTGGCGAGGATTCACCG+TGG | - | tig0032661:35050-35069 | Msa1449630:CDS | 60.0% | |
| TTGGCGAGGATTCACCGTGG+TGG | - | tig0032661:35053-35072 | Msa1449630:CDS | 60.0% | |
| TGGCGAGGATTCACCGTGGT+GGG | - | tig0032661:35054-35073 | Msa1449630:CDS | 60.0% | |
| TCTCACCGCCGTTGCCGTCA+TGG | - | tig0032661:34948-34967 | Msa1449630:CDS | 65.0% | |
| GGTGACCATGACGGCAACGG+CGG | + | tig0032661:34956-34975 | None:intergenic | 65.0% | |
| GGCGGTGACCATGACGGCAA+CGG | + | tig0032661:34959-34978 | None:intergenic | 65.0% | |
| GGCGAGGATTCACCGTGGTG+GGG | - | tig0032661:35055-35074 | Msa1449630:CDS | 65.0% | |
| ! | GGATTCACCGTGGTGGGGTC+AGG | - | tig0032661:35060-35079 | Msa1449630:CDS | 65.0% |
| ! | ACCGTGGTGGGGTCAGGTAC+CGG | - | tig0032661:35066-35085 | Msa1449630:CDS | 65.0% |
| ATCGCGGGCGCGTGAGAGAG+TGG | + | tig0032661:34733-34752 | None:intergenic | 70.0% | |
| GCCGGTACCTGACCCCACCA+CGG | + | tig0032661:35070-35089 | None:intergenic | 70.0% | |
| !! | TGGGGTCAGGTACCGGCCGA+TGG | - | tig0032661:35073-35092 | Msa1449630:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0032661 | gene | 34672 | 35205 | 34672 | ID=Msa1449630;Name=Msa1449630 |
| tig0032661 | mRNA | 34672 | 35205 | 34672 | ID=Msa1449630-mRNA-1;Parent=Msa1449630;Name=Msa1449630-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|177 |
| tig0032661 | exon | 34672 | 35205 | 34672 | ID=Msa1449630-mRNA-1:exon:17033;Parent=Msa1449630-mRNA-1 |
| tig0032661 | CDS | 34672 | 35205 | 34672 | ID=Msa1449630-mRNA-1:cds;Parent=Msa1449630-mRNA-1 |
| Gene Sequence |
| Protein sequence |
