AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1461440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1461440 MtrunA17_Chr4g0036071 64.198 81 28 1 6 86 316 395 4.96e-25 96.7
Msa1461440 MtrunA17_Chr2g0306581 83.333 48 8 0 39 86 1 48 1.57e-21 81.3
Msa1461440 MtrunA17_Chr1g0156051 57.143 77 29 1 11 83 343 419 3.31e-18 77.4
Msa1461440 MtrunA17_Chr6g0463571 55.556 72 17 1 15 86 130 186 8.68e-16 70.1
Msa1461440 MtrunA17_Chr2g0329551 60.345 58 23 0 29 86 199 256 6.06e-15 68.2
Msa1461440 MtrunA17_Chr3g0128781 58.491 53 22 0 34 86 91 143 1.19e-12 61.2
Msa1461440 MtrunA17_Chr3g0107491 64.151 53 19 0 34 86 160 212 2.72e-12 60.5
Msa1461440 MtrunA17_Chr5g0432701 66.667 51 17 0 36 86 144 194 4.71e-12 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1461440 AT4G38900.2 54.118 85 39 0 2 86 345 429 4.93e-22 88.6
Msa1461440 AT4G38900.3 54.118 85 39 0 2 86 345 429 4.93e-22 88.6
Msa1461440 AT2G21230.2 59.722 72 29 0 15 86 334 405 7.18e-20 82.4
Msa1461440 AT2G21230.4 59.722 72 29 0 15 86 334 405 8.84e-20 82.0
Msa1461440 AT2G21230.1 59.722 72 29 0 15 86 334 405 8.84e-20 82.0
Msa1461440 AT2G21230.3 59.722 72 29 0 15 86 334 405 8.87e-20 82.0
Msa1461440 AT4G38900.1 50.549 91 39 1 2 86 345 435 9.09e-17 73.6
Msa1461440 AT2G40620.1 56.522 69 23 1 18 86 122 183 6.35e-15 68.2
Msa1461440 AT1G06070.1 62.745 51 19 0 36 86 195 245 3.31e-14 66.2
Msa1461440 AT2G31370.2 48.750 80 37 1 11 86 157 236 3.50e-14 66.2
Msa1461440 AT2G31370.4 48.750 80 37 1 11 86 157 236 3.50e-14 66.2
Msa1461440 AT2G31370.1 48.750 80 37 1 11 86 157 236 3.50e-14 66.2
Msa1461440 AT2G31370.7 48.750 80 37 1 11 86 157 236 3.50e-14 66.2
Msa1461440 AT2G31370.3 48.750 80 37 1 11 86 157 236 3.50e-14 66.2
Msa1461440 AT2G31370.6 48.750 80 37 1 11 86 157 236 3.50e-14 66.2
Msa1461440 AT2G31370.5 48.750 80 37 1 11 86 157 236 3.63e-14 66.2
Msa1461440 AT1G06850.2 64.706 51 18 0 36 86 133 183 8.57e-14 63.9
Msa1461440 AT1G43700.1 62.500 48 18 0 39 86 182 229 9.22e-14 65.1
Msa1461440 AT1G06850.1 64.706 51 18 0 36 86 133 183 1.77e-13 63.9

Find 80 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTCTAATATCTCTGAATTT+TGG 0.061804 tig0041494:+3348 Msa1461440:intergenic
TTCATTGAACATTGCAAATT+TGG 0.113071 tig0041494:+748 Msa1461440:intergenic
ATAGACATCTATACTATTAT+TGG 0.176193 tig0041494:-473 Msa1461440:three_prime_UTR
TGTGAAGCCACTACACTTTC+TGG 0.210478 tig0041494:-859 Msa1461440:exon
TAGATATTTGTTAATAGAAA+TGG 0.260845 tig0041494:+658 Msa1461440:intergenic
TTCGGAATTGAAGAAAATTA+TGG 0.288273 tig0041494:-1108 Msa1461440:CDS
TGGACAACTTACTTTGTTGC+AGG 0.307186 tig0041494:-839 Msa1461440:three_prime_UTR
ATTCTTCCATTGTTGATAGA+AGG 0.318947 tig0041494:+3391 Msa1461440:intergenic
AGCTTACCCTTCTATCTTCC+AGG 0.320309 tig0041494:+2571 Msa1461440:intergenic
CACTGAAATGAAGGATTAAT+AGG 0.321457 tig0041494:+3468 Msa1461440:intergenic
TTATCTGTCGTGTCGTCTTC+AGG 0.333903 tig0041494:-2972 Msa1461440:intron
GGTCTGAAAGCGAAAGAAAA+AGG 0.341772 tig0041494:+3176 Msa1461440:intergenic
TAGACATCTATACTATTATT+GGG 0.343048 tig0041494:-472 Msa1461440:three_prime_UTR
AAATATGCTAAAAGCATTCA+TGG 0.364398 tig0041494:+631 Msa1461440:intergenic
ACAATGTCAAATTGATGTAT+AGG 0.379468 tig0041494:+444 Msa1461440:intergenic
CAAATTTGGCAGCTCTTGGC+AGG 0.379624 tig0041494:+762 Msa1461440:intergenic
ACCTGCATCGTAATGAATTT+CGG 0.390402 tig0041494:-3047 Msa1461440:five_prime_UTR
AATAGTATAGATGTCTATAA+AGG 0.396403 tig0041494:+478 Msa1461440:intergenic
TTGCTAACCTGGAAGATAGA+AGG 0.399917 tig0041494:-2578 Msa1461440:CDS
CAGTTTGGTGGTGTATGAAT+AGG 0.401258 tig0041494:+3706 Msa1461440:intergenic
TGTAAAACTACTCAAATCAT+AGG 0.405907 tig0041494:+721 Msa1461440:intergenic
AGATATTTGTTAATAGAAAT+GGG 0.408072 tig0041494:+659 Msa1461440:intergenic
ATCTACAGATACCGCAGAAT+TGG 0.415201 tig0041494:+3316 Msa1461440:intergenic
TGATGATGGGGTTGCTAACC+TGG 0.430377 tig0041494:-2589 Msa1461440:CDS
GTACCGGGAGACAATAATAA+TGG 0.441739 tig0041494:+3068 Msa1461440:intergenic
GCGCAAGATGCAATACATTT+CGG 0.444328 tig0041494:-906 Msa1461440:CDS
CACAGAGCCTTTACATGTGC+CGG 0.447228 tig0041494:-3008 Msa1461440:five_prime_UTR
ATTCATTACGATGCAGGTAC+CGG 0.456921 tig0041494:+3052 Msa1461440:intergenic
ACAAAGTCATAAATACAGTT+TGG 0.458891 tig0041494:+3691 Msa1461440:intergenic
CAACACCTCTTAGCCCGCTT+CGG 0.460625 tig0041494:+1050 Msa1461440:intergenic
ATTGCAAATTTGGCAGCTCT+TGG 0.463091 tig0041494:+758 Msa1461440:intergenic
TCCGAAATTCATTACGATGC+AGG 0.464999 tig0041494:+3046 Msa1461440:intergenic
CTTATGTTTGGAATTGATGT+GGG 0.467627 tig0041494:+3272 Msa1461440:intergenic
TAGCACTTGTTCCAGTGCCA+TGG 0.468140 tig0041494:+2638 Msa1461440:intergenic
CCACATCAATTCCAAACATA+AGG 0.469646 tig0041494:-3271 Msa1461440:five_prime_UTR
AGGCTCTGTGAAAGTCGAAA+TGG 0.471589 tig0041494:+3021 Msa1461440:intergenic
TGATGTAATAGAGTATCATT+TGG 0.474450 tig0041494:+3206 Msa1461440:intergenic
ACTCATGCCGGCACATGTAA+AGG 0.475614 tig0041494:+3001 Msa1461440:intergenic
AGCACTTGTTCCAGTGCCAT+GGG 0.478408 tig0041494:+2639 Msa1461440:intergenic
GAAAGTTGCCGAGATTGTTA+TGG 0.488140 tig0041494:-1078 Msa1461440:CDS
ATCTCATGTTAAGAACTAAA+CGG 0.492884 tig0041494:+521 Msa1461440:intergenic
TCCTTCTTCTCATCTTCACC+AGG 0.494836 tig0041494:-3594 Msa1461440:five_prime_UTR
TTGTTGATAGAAGGAATTGT+GGG 0.500721 tig0041494:+3400 Msa1461440:intergenic
GAATAAAATTAAACGTGAAG+TGG 0.508574 tig0041494:+3666 Msa1461440:intergenic
AGCGAAGATGAAGTTGATGA+TGG 0.508679 tig0041494:-2603 Msa1461440:CDS
GAAGACACACACTGAAATGA+AGG 0.518553 tig0041494:+3459 Msa1461440:intergenic
GTTGATGAAGCCCATGGCAC+TGG 0.525777 tig0041494:-2649 Msa1461440:intron
TTATATCTGAAATGGACAAA+AGG 0.526627 tig0041494:-586 Msa1461440:three_prime_UTR
TTAGACTGAGAAGAATGCTT+CGG 0.526884 tig0041494:-1126 Msa1461440:intron
GCCTGGTGAAGATGAGAAGA+AGG 0.530562 tig0041494:+3593 Msa1461440:intergenic
CGGATCAGCCATAACAATCT+CGG 0.532249 tig0041494:+1070 Msa1461440:intergenic
CTGATCCGAAGCGGGCTAAG+AGG 0.536785 tig0041494:-1055 Msa1461440:intron
TTGAAGTAATAGCAATTGCC+TGG 0.537981 tig0041494:+3576 Msa1461440:intergenic
TTTCCATTATTATTGTCTCC+CGG 0.544385 tig0041494:-3071 Msa1461440:five_prime_UTR
ACAACTTACTTTGTTGCAGG+TGG 0.544855 tig0041494:-836 Msa1461440:three_prime_UTR
TTCATTACGATGCAGGTACC+GGG 0.550915 tig0041494:+3053 Msa1461440:intergenic
CCTTATGTTTGGAATTGATG+TGG 0.551186 tig0041494:+3271 Msa1461440:intergenic
TTCAAATAATCCTCAAAACA+TGG 0.555818 tig0041494:+789 Msa1461440:intergenic
GCGAAGATGAAGTTGATGAT+GGG 0.560328 tig0041494:-2602 Msa1461440:CDS
TGTTATGGCTGATCCGAAGC+GGG 0.561676 tig0041494:-1063 Msa1461440:CDS
CAACTTACTTTGTTGCAGGT+GGG 0.580671 tig0041494:-835 Msa1461440:three_prime_UTR
CGCTATTACTGCCAATTCTG+CGG 0.581521 tig0041494:-3327 Msa1461440:five_prime_UTR
TCTTATTGAACATTTAGTGA+AGG 0.581762 tig0041494:-691 Msa1461440:three_prime_UTR
ACAATTCCTTCTATCAACAA+TGG 0.586327 tig0041494:-3397 Msa1461440:five_prime_UTR
CATTTCGGAGCTAGAACACA+AGG 0.586933 tig0041494:-891 Msa1461440:CDS
TGCTAACCTGGAAGATAGAA+GGG 0.589442 tig0041494:-2577 Msa1461440:intron
ATTGTTGATAGAAGGAATTG+TGG 0.593661 tig0041494:+3399 Msa1461440:intergenic
GTATTGCATCTTGCGCACAT+TGG 0.606458 tig0041494:+913 Msa1461440:intergenic
AAGTCATAAATACAGTTTGG+TGG 0.607867 tig0041494:+3694 Msa1461440:intergenic
ATGAATAGGTAGAGTCTGCA+CGG 0.611183 tig0041494:+3720 Msa1461440:intergenic
AAACAGAAGAATATGTGCCA+TGG 0.616319 tig0041494:+3123 Msa1461440:intergenic
TCTCAAATTTCTCAAAACCA+TGG 0.619346 tig0041494:-3140 Msa1461440:five_prime_UTR
TTATGTTTGGAATTGATGTG+GGG 0.625617 tig0041494:+3273 Msa1461440:intergenic
TGAATAGGTAGAGTCTGCAC+GGG 0.628866 tig0041494:+3721 Msa1461440:intergenic
AGATAATCTGATACTCATGC+CGG 0.638016 tig0041494:+2989 Msa1461440:intergenic
GTTGATAGAAGGAATTGTGG+GGG 0.645643 tig0041494:+3402 Msa1461440:intergenic
TTGTTATGGCTGATCCGAAG+CGG 0.656660 tig0041494:-1064 Msa1461440:CDS
TGTTGATAGAAGGAATTGTG+GGG 0.673150 tig0041494:+3401 Msa1461440:intergenic
CGAAGATGAAGTTGATGATG+GGG 0.674501 tig0041494:-2601 Msa1461440:CDS
AAGTTGTCCAGAAAGTGTAG+TGG 0.688059 tig0041494:+852 Msa1461430:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAACATCTATTTAAATATTA+CGG + tig0041494:1782-1801 Msa1461440:intergenic 10.0%
!!! AATTTGAGATTTTCATTATT+GGG + tig0041494:1017-1036 Msa1461440:intergenic 15.0%
!!! AAATTTGAGATTTTCATTAT+TGG + tig0041494:1018-1037 Msa1461440:intergenic 15.0%
!! ATTGAAAACAAAACAAAAAA+AGG + tig0041494:1437-1456 Msa1461440:intergenic 15.0%
!!! ATAGGTTATTAAATTTTGAT+TGG - tig0041494:2230-2249 Msa1461440:intron 15.0%
!! ACAATTCAAATAAAGATAAA+TGG + tig0041494:2463-2482 Msa1461440:intergenic 15.0%
!!! TTTATCTTTATTTGAATTGT+TGG - tig0041494:2463-2482 Msa1461440:intron 15.0%
!! GTTAAATTTATTGTATATTG+TGG - tig0041494:2733-2752 Msa1461440:intron 15.0%
!!! AATAAATGATCTTTTAGTTA+TGG - tig0041494:2849-2868 Msa1461440:intron 15.0%
!!! ATAAATGATCTTTTAGTTAT+GGG - tig0041494:2850-2869 Msa1461440:intron 15.0%
!!! AGATATTTGTTAATAGAAAT+GGG + tig0041494:3512-3531 Msa1461440:intergenic 15.0%
!!! TAGATATTTGTTAATAGAAA+TGG + tig0041494:3513-3532 Msa1461440:intergenic 15.0%
!!! ATTCTTTTTTATATCTGAAA+TGG - tig0041494:3574-3593 Msa1461440:five_prime_UTR 15.0%
!!! AACATTTATGTTTGAAATGA+AGG - tig0041494:653-672 Msa1461440:three_prime_UTR 20.0%
!!! ATTTGAGATTTTCATTATTG+GGG + tig0041494:1016-1035 Msa1461440:intergenic 20.0%
!! AAAACAAAACAAAAAAAGGA+TGG + tig0041494:1433-1452 Msa1461440:intergenic 20.0%
!! TAATTTCTATAGCATAACAT+AGG - tig0041494:2212-2231 Msa1461440:intron 20.0%
!!! TTGAAATTGTTTGATTTGAA+TGG - tig0041494:2582-2601 Msa1461440:CDS 20.0%
!!! TGAAATTGTTTGATTTGAAT+GGG - tig0041494:2583-2602 Msa1461440:CDS 20.0%
!! AAAACATATGTGATTGTATT+AGG - tig0041494:2633-2652 Msa1461440:CDS 20.0%
!! TAAAAGTTATAATTGTCTCT+AGG - tig0041494:2660-2679 Msa1461440:intron 20.0%
!! ATTCTCAAAGTAACATATTA+TGG + tig0041494:2832-2851 Msa1461440:intergenic 20.0%
!! TTTAGTTAGTAATGTCAATA+TGG - tig0041494:3160-3179 Msa1461440:five_prime_UTR 20.0%
!!! ATTTGACTTGGATATTTTTT+TGG - tig0041494:3190-3209 Msa1461440:five_prime_UTR 20.0%
!!! TTTGACTTGGATATTTTTTT+GGG - tig0041494:3191-3210 Msa1461440:five_prime_UTR 20.0%
!!! ATATTTTTTTGGGTTATTTC+AGG - tig0041494:3201-3220 Msa1461440:five_prime_UTR 20.0%
!! AATAGTATAGATGTCTATAA+AGG + tig0041494:3693-3712 Msa1461440:intergenic 20.0%
!! ATAGACATCTATACTATTAT+TGG - tig0041494:3695-3714 Msa1461440:five_prime_UTR 20.0%
!! TAGACATCTATACTATTATT+GGG - tig0041494:3696-3715 Msa1461440:five_prime_UTR 20.0%
! ACAAAGTCATAAATACAGTT+TGG + tig0041494:480-499 Msa0413390:intergenic 25.0%
! GAATAAAATTAAACGTGAAG+TGG + tig0041494:505-524 Msa0413390:intergenic 25.0%
!!! CTGATTTTTTGTTTGAACTT+GGG + tig0041494:550-569 Msa0413390:intergenic 25.0%
!!! TCTGATTTTTTGTTTGAACT+TGG + tig0041494:551-570 Msa0413390:intergenic 25.0%
!! TCTAATATCTCTGAATTTTG+GGG + tig0041494:821-840 Msa1461440:intergenic 25.0%
!! GTCTAATATCTCTGAATTTT+GGG + tig0041494:822-841 Msa1461440:intergenic 25.0%
!!! GGGTGTAATTAGTTTTTTTT+TGG + tig0041494:878-897 Msa1461440:intergenic 25.0%
!! TGATGTAATAGAGTATCATT+TGG + tig0041494:965-984 Msa1461440:intergenic 25.0%
!! CCTTTTAAATTCTGTAATCA+CGG - tig0041494:1372-1391 Msa1461440:intron 25.0%
! ATAAATCAAATGAGCATATC+AGG + tig0041494:1470-1489 Msa1461440:intergenic 25.0%
!!! CAAATTCTGTTTTTCTTCTA+TGG - tig0041494:1632-1651 Msa1461440:intron 25.0%
!!! AGATTTTAGATTGTTACTTC+TGG - tig0041494:1677-1696 Msa1461440:intron 25.0%
!!! GATTTTAGATTGTTACTTCT+GGG - tig0041494:1678-1697 Msa1461440:intron 25.0%
!! CATGTTTTATAGAATGAACA+TGG - tig0041494:1701-1720 Msa1461440:intron 25.0%
! AGAAAAAGATCAAAATGAAC+AGG + tig0041494:2049-2068 Msa1461440:intergenic 25.0%
! ACAAATAGAAAGAGGTAATT+TGG - tig0041494:2118-2137 Msa1461440:intron 25.0%
!!! AGGTAATTTGGATTTTTAGT+AGG - tig0041494:2130-2149 Msa1461440:intron 25.0%
!!! TTTGGATTTTTAGTAGGAAT+TGG - tig0041494:2136-2155 Msa1461440:intron 25.0%
!! AAAAGTTGGTCATTTATGAA+TGG + tig0041494:2344-2363 Msa1461440:intergenic 25.0%
! AAACATAAAGGTTCAAAAGT+TGG + tig0041494:2358-2377 Msa1461440:intergenic 25.0%
! ATCAAACACAACAAACATAA+AGG + tig0041494:2370-2389 Msa1461440:intergenic 25.0%
!! TGTTTGATGTCCATTTTATT+TGG - tig0041494:2382-2401 Msa1461440:intron 25.0%
! GAAAAAGAAGCCAAATAAAA+TGG + tig0041494:2395-2414 Msa1461440:intergenic 25.0%
! ATAAGACGATAAAAATACCA+AGG + tig0041494:2497-2516 Msa1461440:intergenic 25.0%
!! TTATAATTGTCTCTAGGAAT+TGG - tig0041494:2666-2685 Msa1461440:intron 25.0%
! AGGTTAATTACAAAACTAAC+AGG + tig0041494:2778-2797 Msa1461440:intergenic 25.0%
! TTTACTTCTCTTATGTTACT+TGG - tig0041494:2913-2932 Msa1461440:intron 25.0%
! TTCGGAATTGAAGAAAATTA+TGG - tig0041494:3060-3079 Msa1461440:five_prime_UTR 25.0%
! TTCAAATAATCCTCAAAACA+TGG + tig0041494:3382-3401 Msa1461440:intergenic 25.0%
! TTCATTGAACATTGCAAATT+TGG + tig0041494:3423-3442 Msa1461440:intergenic 25.0%
! TGTAAAACTACTCAAATCAT+AGG + tig0041494:3450-3469 Msa1461440:intergenic 25.0%
! TCTTATTGAACATTTAGTGA+AGG - tig0041494:3477-3496 Msa1461440:intron 25.0%
!!! AAATATGCTAAAAGCATTCA+TGG + tig0041494:3540-3559 Msa1461440:intergenic 25.0%
!! TTATATCTGAAATGGACAAA+AGG - tig0041494:3582-3601 Msa1461440:five_prime_UTR 25.0%
!!! AGTTTAGTTTTTAACACAGA+AGG - tig0041494:3615-3634 Msa1461440:five_prime_UTR 25.0%
! ATCTCATGTTAAGAACTAAA+CGG + tig0041494:3650-3669 Msa1461440:intergenic 25.0%
! ACAATGTCAAATTGATGTAT+AGG + tig0041494:3727-3746 Msa1461440:intergenic 25.0%
AAGTCATAAATACAGTTTGG+TGG + tig0041494:477-496 Msa0413390:intergenic 30.0%
! GAAAAAAAAAGTGTAGACTG+AGG + tig0041494:628-647 Msa1461440:intergenic 30.0%
ATAGGAAGTTGAGAATTGAT+AGG + tig0041494:685-704 Msa1461440:intergenic 30.0%
CACTGAAATGAAGGATTAAT+AGG + tig0041494:703-722 Msa1461440:intergenic 30.0%
! TTGTTGATAGAAGGAATTGT+GGG + tig0041494:771-790 Msa1461440:intergenic 30.0%
! ATTGTTGATAGAAGGAATTG+TGG + tig0041494:772-791 Msa1461440:intergenic 30.0%
ACAATTCCTTCTATCAACAA+TGG - tig0041494:771-790 Msa1461440:three_prime_UTR 30.0%
! ATTCTTCCATTGTTGATAGA+AGG + tig0041494:780-799 Msa1461440:intergenic 30.0%
! CGTCTAATATCTCTGAATTT+TGG + tig0041494:823-842 Msa1461440:intergenic 30.0%
! TTATGTTTGGAATTGATGTG+GGG + tig0041494:898-917 Msa1461440:intergenic 30.0%
! CTTATGTTTGGAATTGATGT+GGG + tig0041494:899-918 Msa1461440:intergenic 30.0%
CAAAAACAGTACCTTATGTT+TGG + tig0041494:911-930 Msa1461440:intergenic 30.0%
TCTCAAATTTCTCAAAACCA+TGG - tig0041494:1028-1047 Msa1461440:intron 30.0%
TTTCCATTATTATTGTCTCC+CGG - tig0041494:1097-1116 Msa1461440:CDS 30.0%
CCGTGATTACAGAATTTAAA+AGG + tig0041494:1375-1394 Msa1461440:intergenic 30.0%
AACAACCTTGAATAGTAGAT+AGG - tig0041494:1811-1830 Msa1461440:intron 30.0%
CAATACCTATCTACTATTCA+AGG + tig0041494:1819-1838 Msa1461440:intergenic 30.0%
! GAATTGGTGAAAGTTTTCTA+GGG - tig0041494:2152-2171 Msa1461440:intron 30.0%
!! ATTTGATTTGCAGGTATGAT+TGG - tig0041494:2318-2337 Msa1461440:intron 30.0%
! AATTGTTGGATTTTCTACCT+TGG - tig0041494:2477-2496 Msa1461440:intron 30.0%
TGTCTTATGCTAACATAAGA+TGG + tig0041494:2536-2555 Msa1461440:intergenic 30.0%
ACAAAGAAAGTAACACTTTG+AGG + tig0041494:2715-2734 Msa1461440:intergenic 30.0%
GTTAGTAATGTCAATATGGA+TGG - tig0041494:3164-3183 Msa1461440:five_prime_UTR 30.0%
! TATGGATGGTTAATTTGACT+TGG - tig0041494:3178-3197 Msa1461440:five_prime_UTR 30.0%
!! ATTTCAGGATTTTGAGAAAC+AGG - tig0041494:3216-3235 Msa1461440:five_prime_UTR 30.0%
ACTTGGGAAAAATGATGCTT+TGG + tig0041494:534-553 Msa0413390:intergenic 35.0%
TTGAAGTAATAGCAATTGCC+TGG + tig0041494:595-614 Msa1461440:intergenic 35.0%
! TGTTGATAGAAGGAATTGTG+GGG + tig0041494:770-789 Msa1461440:intergenic 35.0%
!!! ATTTTGGGGTTTCATAGCTT+TGG + tig0041494:807-826 Msa1461440:intergenic 35.0%
! CCTTATGTTTGGAATTGATG+TGG + tig0041494:900-919 Msa1461440:intergenic 35.0%
CCACATCAATTCCAAACATA+AGG - tig0041494:897-916 Msa1461440:CDS 35.0%
GTCTGAAAGCGAAAGAAAAA+GGG + tig0041494:994-1013 Msa1461440:intergenic 35.0%
AAACAGAAGAATATGTGCCA+TGG + tig0041494:1048-1067 Msa1461440:intergenic 35.0%
ACCTGCATCGTAATGAATTT+CGG - tig0041494:1121-1140 Msa1461440:CDS 35.0%
AGATAATCTGATACTCATGC+CGG + tig0041494:1182-1201 Msa1461440:intergenic 35.0%
ACACACAACAAAATGAGCAA+TGG + tig0041494:1501-1520 Msa1461440:intergenic 35.0%
ATAGAATGAACATGGACATG+TGG - tig0041494:1709-1728 Msa1461440:intron 35.0%
TAGAATGAACATGGACATGT+GGG - tig0041494:1710-1729 Msa1461440:intron 35.0%
ACTTGCAGTTTACTGATAAG+TGG - tig0041494:1737-1756 Msa1461440:intron 35.0%
CTTGCAGTTTACTGATAAGT+GGG - tig0041494:1738-1757 Msa1461440:intron 35.0%
TATTGTAGCCGCTCTTTAAT+CGG - tig0041494:1834-1853 Msa1461440:intron 35.0%
TGATGCAAGTAAAAAAGGCA+TGG + tig0041494:1922-1941 Msa1461440:intergenic 35.0%
!!! ATGCCTTTTTTACTTGCATC+AGG - tig0041494:1921-1940 Msa1461440:intron 35.0%
!!! TGCCTTTTTTACTTGCATCA+GGG - tig0041494:1922-1941 Msa1461440:intron 35.0%
TCACATATAGTATAGCTCTC+CGG - tig0041494:1971-1990 Msa1461440:intron 35.0%
TCAACATTGTAATCAAGAGC+CGG + tig0041494:1993-2012 Msa1461440:intergenic 35.0%
CGACATCTACAAATAGAAAG+AGG - tig0041494:2110-2129 Msa1461440:intron 35.0%
! GGAATTGGTGAAAGTTTTCT+AGG - tig0041494:2151-2170 Msa1461440:intron 35.0%
GTATATTGTGGTTGTTTGTG+CGG - tig0041494:2745-2764 Msa1461440:intron 35.0%
AACCTGAGTTCATGCTTTAA+AGG - tig0041494:2793-2812 Msa1461440:intron 35.0%
TTAGACTGAGAAGAATGCTT+TGG - tig0041494:2978-2997 Msa1461440:five_prime_UTR 35.0%
TTAGACTGAGAAGAATGCTT+CGG - tig0041494:2978-2997 Msa1461440:five_prime_UTR 35.0%
!! GATTTTGAGAAACAGGCAAT+CGG - tig0041494:3223-3242 Msa1461440:five_prime_UTR 35.0%
! GTTTGTGAAACCATGTTTTG+AGG - tig0041494:3369-3388 Msa1461440:five_prime_UTR 35.0%
ATGAATAGGTAGAGTCTGCA+CGG + tig0041494:451-470 Msa0413390:intergenic 40.0%
!! CAGTTTGGTGGTGTATGAAT+AGG + tig0041494:465-484 Msa0413390:intergenic 40.0%
GAAGACACACACTGAAATGA+AGG + tig0041494:712-731 Msa1461440:intergenic 40.0%
! GTTGATAGAAGGAATTGTGG+GGG + tig0041494:769-788 Msa1461440:intergenic 40.0%
ATCTACAGATACCGCAGAAT+TGG + tig0041494:855-874 Msa1461440:intergenic 40.0%
GGTCTGAAAGCGAAAGAAAA+AGG + tig0041494:995-1014 Msa1461440:intergenic 40.0%
GTACCGGGAGACAATAATAA+TGG + tig0041494:1103-1122 Msa1461440:intergenic 40.0%
ATTCATTACGATGCAGGTAC+CGG + tig0041494:1119-1138 Msa1461440:intergenic 40.0%
TCCGAAATTCATTACGATGC+AGG + tig0041494:1125-1144 Msa1461440:intergenic 40.0%
!! AGCGAAGATGAAGTTGATGA+TGG - tig0041494:1565-1584 Msa1461440:intron 40.0%
!! GCGAAGATGAAGTTGATGAT+GGG - tig0041494:1566-1585 Msa1461440:intron 40.0%
!! CGAAGATGAAGTTGATGATG+GGG - tig0041494:1567-1586 Msa1461440:intron 40.0%
TTGCTAACCTGGAAGATAGA+AGG - tig0041494:1590-1609 Msa1461440:intron 40.0%
TGCTAACCTGGAAGATAGAA+GGG - tig0041494:1591-1610 Msa1461440:intron 40.0%
AGCCCTGATGCAAGTAAAAA+AGG + tig0041494:1927-1946 Msa1461440:intergenic 40.0%
!! GTGCTACTGATTTGATTTGC+AGG - tig0041494:2309-2328 Msa1461440:intron 40.0%
!! CTGCTCATTTGTGTTGCTTT+AGG - tig0041494:2425-2444 Msa1461440:intron 40.0%
!! CTCATTTGTGTTGCTTTAGG+AGG - tig0041494:2428-2447 Msa1461440:intron 40.0%
!! CTCCTTTAAAGCATGAACTC+AGG + tig0041494:2798-2817 Msa1461440:intergenic 40.0%
!! AAGAATGCTTTGGAGGAAGA+AGG - tig0041494:2988-3007 Msa1461440:five_prime_UTR 40.0%
GAAAGTTGCCGAGATTGTTA+TGG - tig0041494:3090-3109 Msa1461440:five_prime_UTR 40.0%
GCGCAAGATGCAATACATTT+CGG - tig0041494:3262-3281 Msa1461440:five_prime_UTR 40.0%
! AAGTTGTCCAGAAAGTGTAG+TGG + tig0041494:3319-3338 Msa1461440:intergenic 40.0%
TGGACAACTTACTTTGTTGC+AGG - tig0041494:3329-3348 Msa1461440:five_prime_UTR 40.0%
ACAACTTACTTTGTTGCAGG+TGG - tig0041494:3332-3351 Msa1461440:five_prime_UTR 40.0%
CAACTTACTTTGTTGCAGGT+GGG - tig0041494:3333-3352 Msa1461440:five_prime_UTR 40.0%
ATTGCAAATTTGGCAGCTCT+TGG + tig0041494:3413-3432 Msa1461440:intergenic 40.0%
TGAATAGGTAGAGTCTGCAC+GGG + tig0041494:450-469 Msa0413390:intergenic 45.0%
TCCTTCTTCTCATCTTCACC+AGG - tig0041494:574-593 Msa1461440:three_prime_UTR 45.0%
GAATTGTGGGGGAAATTGAG+GGG + tig0041494:758-777 Msa1461440:intergenic 45.0%
GGAATTGTGGGGGAAATTGA+GGG + tig0041494:759-778 Msa1461440:intergenic 45.0%
AGGAATTGTGGGGGAAATTG+AGG + tig0041494:760-779 Msa1461440:intergenic 45.0%
CGCTATTACTGCCAATTCTG+CGG - tig0041494:841-860 Msa1461440:three_prime_UTR 45.0%
TTCATTACGATGCAGGTACC+GGG + tig0041494:1118-1137 Msa1461440:intergenic 45.0%
AGGCTCTGTGAAAGTCGAAA+TGG + tig0041494:1150-1169 Msa1461440:intergenic 45.0%
TTATCTGTCGTGTCGTCTTC+AGG - tig0041494:1196-1215 Msa1461440:intron 45.0%
! TTGTGTGTTGATGAAGCCCA+TGG - tig0041494:1513-1532 Msa1461440:intron 45.0%
AGCTTACCCTTCTATCTTCC+AGG + tig0041494:1600-1619 Msa1461440:intergenic 45.0%
CAGTTTACTGATAAGTGGGC+AGG - tig0041494:1742-1761 Msa1461440:intron 45.0%
CTACCACTCCGATTAAAGAG+CGG + tig0041494:1845-1864 Msa1461440:intergenic 45.0%
! CTTTGAGGACCATGTATTGC+AGG + tig0041494:2700-2719 Msa1461440:intergenic 45.0%
GACTGAGAAGAATGCTTTGG+AGG - tig0041494:2981-3000 Msa1461440:five_prime_UTR 45.0%
CGGATCAGCCATAACAATCT+CGG + tig0041494:3101-3120 Msa1461440:intergenic 45.0%
TTGTTATGGCTGATCCGAAG+CGG - tig0041494:3104-3123 Msa1461440:five_prime_UTR 45.0%
GTATTGCATCTTGCGCACAT+TGG + tig0041494:3258-3277 Msa1461440:intergenic 45.0%
CATTTCGGAGCTAGAACACA+AGG - tig0041494:3277-3296 Msa1461440:five_prime_UTR 45.0%
TGTGAAGCCACTACACTTTC+TGG - tig0041494:3309-3328 Msa1461440:five_prime_UTR 45.0%
GCCTGGTGAAGATGAGAAGA+AGG + tig0041494:578-597 Msa1461440:intergenic 50.0%
CACAGAGCCTTTACATGTGC+CGG - tig0041494:1160-1179 Msa1461440:intron 50.0%
ACTCATGCCGGCACATGTAA+AGG + tig0041494:1170-1189 Msa1461440:intergenic 50.0%
AGCACTTGTTCCAGTGCCAT+GGG + tig0041494:1532-1551 Msa1461440:intergenic 50.0%
TAGCACTTGTTCCAGTGCCA+TGG + tig0041494:1533-1552 Msa1461440:intergenic 50.0%
!! TGATGATGGGGTTGCTAACC+TGG - tig0041494:1579-1598 Msa1461440:intron 50.0%
TAGCCGCTCTTTAATCGGAG+TGG - tig0041494:1839-1858 Msa1461440:intron 50.0%
CGCTCTTTAATCGGAGTGGT+AGG - tig0041494:1843-1862 Msa1461440:intron 50.0%
GAGGACCATGTATTGCAGGT+CGG + tig0041494:2696-2715 Msa1461440:intergenic 50.0%
!! GCTTTGGAGGAAGAAGGATC+TGG - tig0041494:2994-3013 Msa1461440:five_prime_UTR 50.0%
TGTTATGGCTGATCCGAAGC+GGG - tig0041494:3105-3124 Msa1461440:five_prime_UTR 50.0%
CAAATTTGGCAGCTCTTGGC+AGG + tig0041494:3409-3428 Msa1461440:intergenic 50.0%
! GTTGATGAAGCCCATGGCAC+TGG - tig0041494:1519-1538 Msa1461440:intron 55.0%
AGTGGGCAGGTTGCTCAAAC+TGG - tig0041494:1755-1774 Msa1461440:intron 55.0%
TCGGAGTGGTAGGCACAAAC+TGG - tig0041494:1853-1872 Msa1461440:intron 55.0%
GAGAGCCGACCTGCAATACA+TGG - tig0041494:2688-2707 Msa1461440:intron 55.0%
CAACACCTCTTAGCCCGCTT+CGG + tig0041494:3121-3140 Msa1461440:intergenic 55.0%
!! CTGATCCGAAGCGGGCTAAG+AGG - tig0041494:3113-3132 Msa1461440:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
tig0041494 gene 439 3751 439 ID=Msa1461440;Name=Msa1461440
tig0041494 mRNA 439 3751 439 ID=Msa1461440-mRNA-1;Parent=Msa1461440;Name=Msa1461440-mRNA-1;_AED=0.04;_eAED=0.04;_QI=707|0.75|0.8|1|0.5|0.6|5|438|86
tig0041494 exon 439 967 439 ID=Msa1461440-mRNA-1:exon:23197;Parent=Msa1461440-mRNA-1
tig0041494 exon 1056 1144 1056 ID=Msa1461440-mRNA-1:exon:23196;Parent=Msa1461440-mRNA-1
tig0041494 exon 2578 2661 2578 ID=Msa1461440-mRNA-1:exon:23195;Parent=Msa1461440-mRNA-1
tig0041494 exon 2973 3493 2973 ID=Msa1461440-mRNA-1:exon:23194;Parent=Msa1461440-mRNA-1
tig0041494 exon 3569 3751 3569 ID=Msa1461440-mRNA-1:exon:23193;Parent=Msa1461440-mRNA-1
tig0041494 five_prime_UTR 3569 3751 3569 ID=Msa1461440-mRNA-1:five_prime_utr;Parent=Msa1461440-mRNA-1
tig0041494 five_prime_UTR 2973 3493 2973 ID=Msa1461440-mRNA-1:five_prime_utr;Parent=Msa1461440-mRNA-1
tig0041494 five_prime_UTR 2659 2661 2659 ID=Msa1461440-mRNA-1:five_prime_utr;Parent=Msa1461440-mRNA-1
tig0041494 CDS 2578 2658 2578 ID=Msa1461440-mRNA-1:cds;Parent=Msa1461440-mRNA-1
tig0041494 CDS 1056 1144 1056 ID=Msa1461440-mRNA-1:cds;Parent=Msa1461440-mRNA-1
tig0041494 CDS 877 967 877 ID=Msa1461440-mRNA-1:cds;Parent=Msa1461440-mRNA-1
tig0041494 three_prime_UTR 439 876 439 ID=Msa1461440-mRNA-1:three_prime_utr;Parent=Msa1461440-mRNA-1
Gene Sequence

>Msa1461440

ATGGCACTGGAACAAGTGCTATCAGACTGTCATAGCGAAGATGAAGTTGATGATGGGGTTGCTAACCTGGAAGATAGAAGGACTGAGAAGAATGCTTCGGAATTGAAGAAAATTATGGCTAATGAGAAAGTTGCCGAGATTGTTATGGCTGATCCGAAGCGGGCTAAGAGGATTTTGAGAAACAGGCAATCGGCTGCGCATTCCAATGTGCGCAAGATGCAATACATTTCGGAGCTAGAACACAAGGTTCAGACTCTGTGA
Protein sequence

>Msa1461440

MALEQVLSDCHSEDEVDDGVANLEDRRTEKNASELKKIMANEKVAEIVMADPKRAKRILRNRQSAAHSNVRKMQYISELEHKVQTL*