AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene00086

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00086.t1 MTR_2g032710 96.491 456 9 2 1 455 14 463 0.0 904
MS.gene00086.t1 MTR_4g094715 73.465 456 111 2 1 455 15 461 0.0 693
MS.gene00086.t1 MTR_1g086390 63.265 392 142 1 56 445 78 469 0.0 549
MS.gene00086.t1 MTR_7g110050 60.142 424 160 4 32 453 46 462 0.0 539
MS.gene00086.t1 MTR_5g034090 46.711 456 204 9 18 443 77 523 1.46e-132 393
MS.gene00086.t1 MTR_8g065010 47.897 428 188 7 41 442 91 509 1.03e-130 388
MS.gene00086.t1 MTR_6g005630 46.056 393 194 6 53 444 39 414 6.43e-120 357
MS.gene00086.t1 MTR_5g033680 41.439 403 218 8 43 440 50 439 2.30e-106 323
MS.gene00086.t1 MTR_6g005630 43.003 393 183 6 53 444 39 391 1.05e-105 320
MS.gene00086.t1 MTR_8g065030 39.206 403 225 7 58 451 64 455 1.17e-95 296
MS.gene00086.t1 MTR_1g052315 43.889 360 182 10 54 406 57 403 4.83e-89 278
MS.gene00086.t1 MTR_2g103410 39.842 379 215 7 59 434 59 427 1.19e-88 277
MS.gene00086.t1 MTR_5g034090 45.970 335 145 7 18 323 77 404 4.30e-87 272
MS.gene00086.t1 MTR_7g091850 41.620 358 196 5 58 412 75 422 1.34e-85 270
MS.gene00086.t1 MTR_2g060350 40.000 355 198 7 61 411 76 419 6.27e-81 257
MS.gene00086.t1 MTR_7g091840 40.854 328 180 5 54 376 60 378 4.62e-80 253
MS.gene00086.t1 MTR_2g103650 34.615 390 243 7 52 437 21 402 3.31e-74 239
MS.gene00086.t1 MTR_6g028080 36.598 388 217 9 59 437 30 397 4.79e-74 238
MS.gene00086.t1 MTR_1g012390 37.143 385 223 9 59 439 28 397 6.24e-72 233
MS.gene00086.t1 MTR_3g030540 37.903 372 207 8 51 416 62 415 8.27e-70 228
MS.gene00086.t1 MTR_3g054320 35.714 392 226 10 59 445 30 400 2.50e-69 226
MS.gene00086.t1 MTR_3g084380 34.722 360 215 6 57 411 19 363 7.75e-67 219
MS.gene00086.t1 MTR_2g049400 35.325 385 232 7 54 437 23 391 8.15e-66 216
MS.gene00086.t1 MTR_2g058670 34.831 356 220 7 54 406 20 366 1.16e-65 217
MS.gene00086.t1 MTR_8g010080 35.309 388 222 10 53 437 25 386 1.00e-64 213
MS.gene00086.t1 MTR_2g058640 36.056 355 213 8 56 406 22 366 3.87e-64 213
MS.gene00086.t1 MTR_8g010070 34.359 390 227 11 54 439 26 390 5.09e-64 212
MS.gene00086.t1 MTR_3g438050 34.048 373 227 7 56 423 24 382 7.34e-64 211
MS.gene00086.t1 MTR_8g010040 35.309 388 219 9 54 436 27 387 8.21e-64 211
MS.gene00086.t1 MTR_4g084050 34.521 365 212 8 59 412 23 371 1.19e-63 211
MS.gene00086.t1 MTR_2g058840 34.160 363 211 8 54 406 20 364 1.41e-63 211
MS.gene00086.t1 MTR_1g088160 35.263 380 218 13 54 422 30 392 7.04e-63 209
MS.gene00086.t1 MTR_3g084360 34.262 359 216 8 59 412 24 367 1.34e-62 208
MS.gene00086.t1 MTR_0157s0060 34.817 382 221 8 64 439 3 362 4.47e-62 206
MS.gene00086.t1 MTR_4g034020 34.817 382 221 8 64 439 3 362 4.47e-62 206
MS.gene00086.t1 MTR_5g097860 37.349 332 191 7 52 378 18 337 4.78e-62 205
MS.gene00086.t1 MTR_8g008765 35.671 328 186 5 54 378 26 331 7.45e-62 204
MS.gene00086.t1 MTR_4g083660 34.794 388 232 11 54 437 4 374 8.70e-62 205
MS.gene00086.t1 MTR_8g010110 33.075 387 231 8 54 437 26 387 1.19e-61 205
MS.gene00086.t1 MTR_5g043350 33.418 392 236 10 57 441 27 400 4.00e-61 204
MS.gene00086.t1 MTR_8g010090 33.333 387 230 9 54 437 26 387 1.59e-60 202
MS.gene00086.t1 MTR_7g035415 36.278 317 178 7 56 367 37 334 1.65e-60 201
MS.gene00086.t1 MTR_7g113910 35.772 369 150 8 58 424 56 339 8.44e-60 200
MS.gene00086.t1 MTR_2g103410 37.453 267 157 4 59 323 59 317 3.94e-58 194
MS.gene00086.t1 MTR_2g435640 34.626 361 217 8 51 406 48 394 4.74e-58 197
MS.gene00086.t1 MTR_8g009810 35.196 358 213 9 59 412 35 377 1.22e-57 195
MS.gene00086.t1 MTR_8g030930 33.518 361 218 9 56 408 24 370 4.03e-56 191
MS.gene00086.t1 MTR_1g013730 32.984 382 225 9 59 437 27 380 5.58e-55 187
MS.gene00086.t1 MTR_4g120320 33.162 389 240 10 51 434 47 420 4.38e-54 186
MS.gene00086.t1 MTR_1g013740 32.552 384 228 9 59 439 35 390 4.55e-54 186
MS.gene00086.t1 MTR_2g029170 34.706 340 203 10 101 437 38 361 1.13e-52 181
MS.gene00086.t1 MTR_8g010050 30.412 388 207 11 54 437 26 354 2.45e-52 180
MS.gene00086.t1 MTR_8g077320 29.602 402 206 9 50 436 24 363 4.81e-48 169
MS.gene00086.t1 MTR_8g008700 33.746 323 167 8 117 437 13 290 1.02e-47 166
MS.gene00086.t1 MTR_5g063430 30.671 313 191 8 53 358 19 312 1.49e-41 150
MS.gene00086.t1 MTR_2g100040 31.476 359 180 12 53 405 19 317 3.15e-40 148
MS.gene00086.t1 MTR_8g031180 36.538 208 122 3 206 407 70 273 5.55e-39 142
MS.gene00086.t1 MTR_4g124980 26.425 386 225 13 58 406 73 436 2.05e-21 97.1
MS.gene00086.t1 MTR_2g042740 25.789 380 235 12 58 406 55 418 8.37e-21 95.1
MS.gene00086.t1 MTR_3g449590 23.515 421 278 10 51 442 39 444 1.44e-17 85.1
MS.gene00086.t1 MTR_5g090160 25.879 313 191 9 64 348 49 348 4.60e-16 80.5
MS.gene00086.t1 MTR_1g100040 25.740 338 204 12 35 343 36 355 6.22e-15 77.0
MS.gene00086.t1 MTR_1g100050 29.020 255 149 8 39 275 32 272 6.88e-15 77.0
MS.gene00086.t1 MTR_2g435340 26.074 326 187 13 55 346 34 339 1.88e-14 75.5
MS.gene00086.t1 MTR_8g098840 24.286 350 211 13 61 375 46 376 2.14e-14 75.5
MS.gene00086.t1 MTR_7g088550 25.649 308 186 10 64 342 55 348 5.32e-14 74.3
MS.gene00086.t1 MTR_1g099850 25.243 309 188 10 64 343 60 354 1.31e-13 73.2
MS.gene00086.t1 MTR_1g100060 27.826 230 136 6 64 275 98 315 2.73e-13 72.0
MS.gene00086.t1 MTR_7g110710 26.140 329 195 11 55 352 40 351 3.00e-13 72.0
MS.gene00086.t1 MTR_7g110710 26.444 329 194 11 55 352 40 351 3.85e-13 71.6
MS.gene00086.t1 MTR_3g096330 29.000 200 116 6 157 343 189 375 5.42e-13 71.6
MS.gene00086.t1 MTR_8g028725 24.058 345 218 11 61 375 57 387 9.18e-13 70.5
MS.gene00086.t1 MTR_1g086030 25.610 328 197 11 55 352 79 389 2.11e-12 69.3
MS.gene00086.t1 MTR_7g078680 23.373 415 266 12 64 445 60 455 5.18e-12 68.2
MS.gene00086.t1 MTR_2g435340 27.230 213 132 6 147 347 74 275 8.09e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00086.t1 AT1G48100 70.601 449 124 4 4 447 21 466 0.0 649
MS.gene00086.t1 AT1G02460 61.893 391 144 3 58 443 88 478 0.0 519
MS.gene00086.t1 AT4G01890 53.167 442 188 4 3 439 23 450 1.83e-168 482
MS.gene00086.t1 AT1G56710 55.335 403 175 3 44 443 33 433 3.39e-168 480
MS.gene00086.t1 AT1G60590 45.411 414 193 6 56 442 120 527 2.39e-121 364
MS.gene00086.t1 AT1G10640 45.499 411 194 6 56 442 116 520 1.78e-120 362
MS.gene00086.t1 AT5G14650 45.885 401 201 6 56 453 48 435 1.19e-117 352
MS.gene00086.t1 AT3G26610 41.043 441 234 8 7 439 27 449 3.69e-107 326
MS.gene00086.t1 AT1G70500 41.000 400 223 6 54 451 62 450 4.30e-100 308
MS.gene00086.t1 AT1G80170 41.425 379 210 6 58 433 52 421 7.96e-99 303
MS.gene00086.t1 AT1G23460 41.388 389 211 7 53 437 37 412 8.21e-96 295
MS.gene00086.t1 AT1G23460 41.388 389 211 7 53 437 61 436 1.16e-95 296
MS.gene00086.t1 AT3G57510 41.573 356 193 6 55 406 64 408 1.71e-87 274
MS.gene00086.t1 AT2G41850 41.061 358 195 7 53 406 63 408 3.18e-86 271
MS.gene00086.t1 AT3G07970 39.362 376 211 9 51 422 64 426 2.35e-82 261
MS.gene00086.t1 AT5G14650 43.866 269 139 3 56 323 48 305 2.64e-72 231
MS.gene00086.t1 AT3G15720 37.500 392 218 10 54 438 19 390 3.13e-71 233
MS.gene00086.t1 AT3G15720 37.500 392 218 10 54 438 18 389 3.85e-71 232
MS.gene00086.t1 AT1G80140 36.286 350 205 6 91 437 2 336 1.01e-70 228
MS.gene00086.t1 AT4G35670 34.551 356 215 6 59 411 24 364 2.05e-69 226
MS.gene00086.t1 AT3G15720 37.500 392 215 11 54 438 18 386 1.39e-68 225
MS.gene00086.t1 AT1G05660 34.447 389 235 10 54 439 23 394 2.16e-66 218
MS.gene00086.t1 AT2G43880 35.714 378 221 9 59 432 31 390 5.10e-66 217
MS.gene00086.t1 AT2G43870 37.892 351 201 7 59 406 21 357 7.30e-66 216
MS.gene00086.t1 AT5G44840 33.067 375 225 8 58 425 23 378 1.56e-65 216
MS.gene00086.t1 AT1G80170 40.299 268 150 4 58 323 52 311 8.92e-65 212
MS.gene00086.t1 AT2G43890 35.340 382 229 11 53 432 23 388 2.26e-64 213
MS.gene00086.t1 AT1G05650 34.271 391 235 10 53 439 22 394 3.80e-64 212
MS.gene00086.t1 AT1G78400 35.567 388 226 8 58 438 34 404 4.53e-64 212
MS.gene00086.t1 AT1G65570 37.500 384 218 12 59 437 31 397 5.24e-64 212
MS.gene00086.t1 AT3G59850 36.798 356 205 10 56 406 20 360 5.16e-63 209
MS.gene00086.t1 AT3G59850 36.798 356 205 10 56 406 64 404 1.29e-62 209
MS.gene00086.t1 AT5G27530 33.889 360 208 7 58 412 52 386 4.36e-62 209
MS.gene00086.t1 AT2G33160 35.369 393 229 9 47 432 25 399 9.14e-62 213
MS.gene00086.t1 AT1G02790 36.338 355 199 9 57 406 50 382 1.32e-61 206
MS.gene00086.t1 AT1G17150 36.053 380 226 8 58 433 32 398 2.20e-61 205
MS.gene00086.t1 AT2G43860 36.176 387 226 12 57 439 31 400 8.43e-61 204
MS.gene00086.t1 AT5G48140 35.398 339 200 9 72 406 34 357 5.18e-60 201
MS.gene00086.t1 AT3G07830 37.168 339 195 9 72 406 34 358 1.76e-59 200
MS.gene00086.t1 AT3G07840 35.613 351 209 8 64 411 26 362 3.44e-59 199
MS.gene00086.t1 AT3G15720 36.919 344 191 9 101 438 3 326 4.63e-58 196
MS.gene00086.t1 AT4G13760 34.270 356 214 9 58 406 30 372 2.01e-57 195
MS.gene00086.t1 AT1G43080 34.551 356 213 9 58 406 30 372 4.77e-57 194
MS.gene00086.t1 AT3G07820 36.337 344 200 10 72 411 33 361 6.03e-57 193
MS.gene00086.t1 AT1G43090 34.831 356 212 9 58 406 30 372 1.91e-56 193
MS.gene00086.t1 AT4G18180 36.288 361 207 10 56 406 34 381 5.24e-56 191
MS.gene00086.t1 AT5G17200 31.643 414 233 12 52 439 32 421 1.07e-55 191
MS.gene00086.t1 AT2G43880 37.162 296 172 5 59 353 31 313 1.20e-55 188
MS.gene00086.t1 AT5G44830 31.765 340 202 6 89 425 7 319 1.72e-55 187
MS.gene00086.t1 AT3G07850 36.944 360 209 8 52 407 63 408 2.56e-55 190
MS.gene00086.t1 AT3G14040 37.677 353 202 8 59 407 71 409 3.85e-55 190
MS.gene00086.t1 AT1G43100 34.734 357 211 10 58 406 30 372 7.35e-55 189
MS.gene00086.t1 AT5G39910 30.000 420 208 12 36 438 19 369 9.05e-55 187
MS.gene00086.t1 AT4G32380 32.162 370 226 7 71 432 39 391 4.76e-54 187
MS.gene00086.t1 AT2G15460 34.637 358 210 11 58 406 30 372 4.95e-54 186
MS.gene00086.t1 AT2G26620 34.181 354 215 9 58 406 30 370 9.37e-54 185
MS.gene00086.t1 AT2G15450 34.358 358 211 11 58 406 30 372 1.06e-53 185
MS.gene00086.t1 AT2G40310 33.894 357 212 10 59 406 31 372 2.80e-53 184
MS.gene00086.t1 AT2G15470 34.078 358 212 11 58 406 30 372 5.07e-52 181
MS.gene00086.t1 AT4G32375 36.655 281 151 6 133 406 5 265 1.43e-51 180
MS.gene00086.t1 AT4G32370 36.177 293 165 6 73 357 49 327 1.21e-49 172
MS.gene00086.t1 AT4G35670 32.841 271 168 3 141 411 1 257 5.97e-49 169
MS.gene00086.t1 AT4G32380 34.944 269 154 5 71 334 39 291 9.45e-45 161
MS.gene00086.t1 AT3G42950 28.169 426 256 18 58 450 65 473 1.06e-21 97.8
MS.gene00086.t1 AT5G41870 27.246 334 186 12 51 348 37 349 2.78e-21 96.3
MS.gene00086.t1 AT1G19170 24.737 380 240 11 58 406 86 450 5.27e-20 92.8
MS.gene00086.t1 AT3G62110 26.888 331 190 13 55 352 40 351 3.40e-17 84.3
MS.gene00086.t1 AT3G62110 26.888 331 190 13 55 352 40 351 3.40e-17 84.3
MS.gene00086.t1 AT4G33440 25.836 329 191 11 46 343 65 371 6.04e-17 83.6
MS.gene00086.t1 AT4G23820 26.708 322 182 12 61 348 43 344 2.04e-16 81.6
MS.gene00086.t1 AT2G23900 23.980 392 242 14 64 421 61 430 1.08e-14 76.6
MS.gene00086.t1 AT3G48950 22.923 349 212 11 64 375 50 378 1.37e-14 76.3
MS.gene00086.t1 AT4G23500 24.096 415 265 13 64 446 73 469 2.56e-12 69.3
MS.gene00086.t1 AT3G06770 26.708 322 190 13 55 346 41 346 6.40e-11 64.7
MS.gene00086.t1 AT3G06770 26.708 322 190 13 55 346 40 345 8.33e-11 64.3

Find 108 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCTCCACTTGATTGTTTA+AGG 0.078821 2.1:+59702992 MS.gene00086:CDS
CTTGATCAAATGACTCTAAA+TGG 0.174861 2.1:+59700632 MS.gene00086:CDS
GGAGATTCTCCAGGATTATT+TGG 0.177480 2.1:-59699938 None:intergenic
GAATATGAATGCAATAATTA+AGG 0.201144 2.1:+59703048 MS.gene00086:CDS
GATAACTCCACAACTGTTTC+AGG 0.213781 2.1:-59703024 None:intergenic
GGTACCTTCCTTCCACATTA+TGG 0.224070 2.1:-59699811 None:intergenic
GTTCTTCATGAGCTCAAATT+TGG 0.241900 2.1:+59700981 MS.gene00086:CDS
CCATAAGCATTCCAACAAAA+AGG 0.243640 2.1:-59702949 None:intergenic
TCTCCAGGATTATTTGGATA+TGG 0.275950 2.1:-59699932 None:intergenic
GCAGCTTGTGCAGTTGATTC+AGG 0.281433 2.1:+59700046 MS.gene00086:CDS
GGATTATTTGGATATGGATA+TGG 0.286489 2.1:-59699926 None:intergenic
GTGCTCCTACCCACGGAATT+AGG 0.301522 2.1:+59701341 MS.gene00086:CDS
CAATTCCATGTGAAATTTGA+TGG 0.330222 2.1:+59701036 MS.gene00086:CDS
TCTGCCTCAGGCCAACAATT+TGG 0.335193 2.1:-59700578 None:intergenic
ATCTTCCTTGCAAACCTCAT+AGG 0.364129 2.1:+59700693 MS.gene00086:CDS
AGTTTCTTACATTCTCCATT+TGG 0.366739 2.1:-59702711 None:intergenic
TATCTTGTTTATAGATGATA+AGG 0.370877 2.1:+59700959 MS.gene00086:intron
TCCTCCACTTGATTGTTTAA+GGG 0.382641 2.1:+59702993 MS.gene00086:CDS
TTCGACAGCATTGGAAGCCT+TGG 0.386798 2.1:+59702553 MS.gene00086:intron
GGCCAACAATTTGGTCCATC+TGG 0.390506 2.1:-59700569 None:intergenic
AGCTCAAATTTGGTGTTAAG+AGG 0.391670 2.1:+59700991 MS.gene00086:CDS
TGACTGAGTTGCGGACTGTT+AGG 0.400859 2.1:-59702606 None:intergenic
GGTGATGATTGCATTTCAAT+TGG 0.404860 2.1:+59701280 MS.gene00086:intron
ATTATTATTATTACCCTATG+AGG 0.418598 2.1:-59700707 None:intergenic
TTATTTACCATTGCTTATCA+AGG 0.419053 2.1:-59701166 None:intergenic
AAGTGGATTCCATCAGTATT+GGG 0.420148 2.1:-59701111 None:intergenic
TCAGTATTGGGACTAAGTTT+AGG 0.423481 2.1:-59701099 None:intergenic
ACTAAGTTTAGGTGCAGAAA+TGG 0.423584 2.1:-59701088 None:intergenic
AATATGAATGCAATAATTAA+GGG 0.424968 2.1:+59703049 MS.gene00086:CDS
CATCCCTTGTGTTTCCTAAG+TGG 0.427078 2.1:-59701128 None:intergenic
TTGAAATATATGTAGATTGA+TGG 0.430307 2.1:+59700533 MS.gene00086:intron
GGTGAAGGGTCACTAGGTGC+TGG 0.431729 2.1:-59699860 None:intergenic
CGTACATCGAAAACGCTTGA+AGG 0.435739 2.1:-59699959 None:intergenic
GGAGGTACAGGATCAGTAAC+AGG 0.441303 2.1:+59702670 MS.gene00086:CDS
TAAGTGGATTCCATCAGTAT+TGG 0.443227 2.1:-59701112 None:intergenic
ACTAGGAGATGTAGGAGGAT+TGG 0.444391 2.1:-59699891 None:intergenic
TTACATATACTGCTGATGTT+TGG 0.447172 2.1:-59702780 None:intergenic
AACATTTGACAACATCCAAA+TGG 0.452717 2.1:+59702696 MS.gene00086:CDS
TCAAATTTCACATGGAATTG+AGG 0.453281 2.1:-59701033 None:intergenic
TTGATCAAATGACTCTAAAT+GGG 0.457341 2.1:+59700633 MS.gene00086:CDS
GTAGATTGATGGTACTTTGA+TGG 0.462628 2.1:+59700544 MS.gene00086:intron
GATTGCATTTCAATTGGTCC+TGG 0.464171 2.1:+59701286 MS.gene00086:CDS
CACCAGATGGACCAAATTGT+TGG 0.467082 2.1:+59700567 MS.gene00086:CDS
CTTACTTGAAATACCAGTCC+TGG 0.467508 2.1:-59700136 None:intergenic
GGATTGGAAGAAGGTGGTGA+AGG 0.469757 2.1:-59699875 None:intergenic
GTGAAGGGTCACTAGGTGCT+GGG 0.472007 2.1:-59699859 None:intergenic
AAAACATGGCAAGGAGGTAC+AGG 0.486274 2.1:+59702658 MS.gene00086:CDS
TCAAGTAATTCACCTTCATA+AGG 0.493532 2.1:-59702922 None:intergenic
TCACTACAAGCAAAGTGTAT+TGG 0.494006 2.1:-59702853 None:intergenic
TATCCATATCCAAATAATCC+TGG 0.495114 2.1:+59699929 MS.gene00086:CDS
TGGTCCATGCAAACCAGGAC+TGG 0.506011 2.1:+59700123 MS.gene00086:CDS
GAGGGAACACTAGGAGATGT+AGG 0.508733 2.1:-59699899 None:intergenic
AATACCAGTCCTGGTTTGCA+TGG 0.514150 2.1:-59700127 None:intergenic
GATGTAGGAGGATTGGAAGA+AGG 0.516798 2.1:-59699884 None:intergenic
TCTTCCTTGCAAACCTCATA+GGG 0.518124 2.1:+59700694 MS.gene00086:CDS
GTACGATCATTCGGAGCAGT+TGG 0.525889 2.1:+59699977 MS.gene00086:CDS
AATACTGATGGAATCCACTT+AGG 0.526638 2.1:+59701114 MS.gene00086:CDS
CAAATGACTCTAAATGGGAC+AGG 0.527686 2.1:+59700638 MS.gene00086:CDS
AAACTTAGTCCCAATACTGA+TGG 0.527825 2.1:+59701102 MS.gene00086:CDS
CCACTTGATTGTTTAAGGGA+AGG 0.528218 2.1:+59702997 MS.gene00086:CDS
AATGGGACAGGAATTATTGA+AGG 0.532465 2.1:+59700650 MS.gene00086:CDS
GATTGGAAGAAGGTGGTGAA+GGG 0.533569 2.1:-59699874 None:intergenic
ACGCTTGAAGGAGATTCTCC+AGG 0.538734 2.1:-59699947 None:intergenic
GGATATGGATATGGATCAGA+GGG 0.546147 2.1:-59699917 None:intergenic
GTCTATAAAACACAAGCCAT+TGG 0.548487 2.1:-59700610 None:intergenic
CTACAAGCAAAGTGTATTGG+AGG 0.549583 2.1:-59702850 None:intergenic
ACAGGAATTATTGAAGGCAA+TGG 0.554020 2.1:+59700656 MS.gene00086:CDS
GAAGGTGGTGAAGGGTCACT+AGG 0.554808 2.1:-59699866 None:intergenic
GCTCAAATTTGGTGTTAAGA+GGG 0.555873 2.1:+59700992 MS.gene00086:CDS
GTAGGAGGATTGGAAGAAGG+TGG 0.559194 2.1:-59699881 None:intergenic
TTAGGAAACACAAGGGATGT+TGG 0.561840 2.1:+59701132 MS.gene00086:CDS
TATAATTCCTTGATAAGCAA+TGG 0.563522 2.1:+59701159 MS.gene00086:CDS
GTGAAATTTGATGGTTGTCA+AGG 0.563909 2.1:+59701045 MS.gene00086:CDS
ATGTGACATACCTAATTCCG+TGG 0.569578 2.1:-59701351 None:intergenic
TCATCCATAATGTGGAAGGA+AGG 0.585473 2.1:+59699807 MS.gene00086:CDS
TATGGATCAGAGGGAACACT+AGG 0.586071 2.1:-59699908 None:intergenic
GTTACTTGTGCTCCTACCCA+CGG 0.586560 2.1:+59701334 MS.gene00086:CDS
TCATTCGGAGCAGTTGGAGA+TGG 0.586682 2.1:+59699983 MS.gene00086:CDS
ATTACCCTATGAGGTTTGCA+AGG 0.592983 2.1:-59700698 None:intergenic
CGTGGGAGTTGTGTACTCCA+AGG 0.597477 2.1:-59702570 None:intergenic
CTAAGAATCAAAACATGGCA+AGG 0.597482 2.1:+59702649 MS.gene00086:CDS
AGGTGAGCCTGAAACAGTTG+TGG 0.597649 2.1:+59703017 MS.gene00086:CDS
TGAAGGGTCACTAGGTGCTG+GGG 0.603447 2.1:-59699858 None:intergenic
AAGGCAATGGAGAACAATGG+TGG 0.608620 2.1:+59700669 MS.gene00086:CDS
ACATTCACATCTGAACATCC+AGG 0.609157 2.1:-59701304 None:intergenic
TGGATATGGATATGGATCAG+AGG 0.617965 2.1:-59699918 None:intergenic
TGTTCAGATGTGAATGTGGA+TGG 0.618209 2.1:+59701310 MS.gene00086:CDS
GTCATAAAAGAATCAGACAA+TGG 0.619796 2.1:+59702625 MS.gene00086:CDS
TCCCTTAAACAATCAAGTGG+AGG 0.624213 2.1:-59702994 None:intergenic
AGGCAATGGAGAACAATGGT+GGG 0.624461 2.1:+59700670 MS.gene00086:CDS
GACAACCATCAAATTTCACA+TGG 0.633858 2.1:-59701041 None:intergenic
AGGCAGACAGTAAAAGCCAA+TGG 0.642020 2.1:+59700594 MS.gene00086:CDS
CCTTCCCTTAAACAATCAAG+TGG 0.643008 2.1:-59702997 None:intergenic
GGTACTTTGATGGCACCAGA+TGG 0.645534 2.1:+59700554 MS.gene00086:CDS
TGGACCAAATTGTTGGCCTG+AGG 0.650727 2.1:+59700574 MS.gene00086:CDS
ACATACCTAATTCCGTGGGT+AGG 0.654879 2.1:-59701346 None:intergenic
GAATCCACTTAGGAAACACA+AGG 0.655820 2.1:+59701124 MS.gene00086:CDS
TGGATGTTCAGATGTGAATG+TGG 0.656226 2.1:+59701306 MS.gene00086:CDS
TTGAAGGCAATGGAGAACAA+TGG 0.660810 2.1:+59700666 MS.gene00086:CDS
TTAGGTTCGAGACACATGCG+TGG 0.677550 2.1:-59702588 None:intergenic
GGAACACTAGGAGATGTAGG+AGG 0.682778 2.1:-59699896 None:intergenic
CTCACTGTAGGGCTAACACA+TGG 0.682946 2.1:-59700844 None:intergenic
CAGCTGCATTTAGAGCAGCA+TGG 0.685070 2.1:+59700020 MS.gene00086:CDS
TGTGACATACCTAATTCCGT+GGG 0.685322 2.1:-59701350 None:intergenic
TAGGTTCGAGACACATGCGT+GGG 0.689252 2.1:-59702587 None:intergenic
ATGGACTAAGAATCAAAACA+TGG 0.703229 2.1:+59702644 MS.gene00086:CDS
AGAATCAAAACATGGCAAGG+AGG 0.705826 2.1:+59702652 MS.gene00086:CDS
AATCCACTTAGGAAACACAA+GGG 0.771508 2.1:+59701125 MS.gene00086:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATAAATAAATAAATAA+TGG + chr2.1:59701616-59701635 MS.gene00086:intron 0.0%
!! ATAGATAAATACAAAAATAA+TGG - chr2.1:59700750-59700769 None:intergenic 10.0%
!! CAATTAATTATGATTAATTT+AGG + chr2.1:59701412-59701431 MS.gene00086:intron 10.0%
!! TCATAAGAAAAAAAATTATA+AGG - chr2.1:59700937-59700956 None:intergenic 10.0%
!!! AATGTTACATTTTATTTAAT+AGG + chr2.1:59701259-59701278 MS.gene00086:intron 10.0%
!!! ATTTTAAAACAGTAATTTTT+AGG - chr2.1:59700085-59700104 None:intergenic 10.0%
!!! ATTTTTCTTCAAATTTATTT+TGG - chr2.1:59702230-59702249 None:intergenic 10.0%
!! AATGTTAAAAACTAAAATTC+TGG + chr2.1:59700772-59700791 MS.gene00086:intron 15.0%
!!! AATATCGAAATTTTATTCTA+TGG + chr2.1:59701213-59701232 MS.gene00086:intron 15.0%
!!! AATTAAATTTTACACAATGA+TGG - chr2.1:59700490-59700509 None:intergenic 15.0%
!!! ATCAAATTTGAATTTTTTAG+AGG + chr2.1:59701952-59701971 MS.gene00086:intron 15.0%
!!! CAAAACTTATTTTATATGTT+TGG + chr2.1:59701977-59701996 MS.gene00086:intron 15.0%
!!! TCTGTTATTTTAGTTAAATT+GGG + chr2.1:59701378-59701397 MS.gene00086:intron 15.0%
!!! TGTAATTATTTTATGTTGTA+TGG + chr2.1:59702363-59702382 MS.gene00086:intron 15.0%
!!! TTCTGTTATTTTAGTTAAAT+TGG + chr2.1:59701377-59701396 MS.gene00086:intron 15.0%
!! ACCTTGTTAGTTAATTATAT+AGG - chr2.1:59700514-59700533 None:intergenic 20.0%
!! ATTATTATTATTACCCTATG+AGG - chr2.1:59700710-59700729 None:intergenic 20.0%
!! TCCTATATAATTAACTAACA+AGG + chr2.1:59700510-59700529 MS.gene00086:intron 20.0%
!! TTGAAATATATGTAGATTGA+TGG + chr2.1:59700533-59700552 MS.gene00086:intron 20.0%
!!! CTTTAAATTTTAGACAAATG+TGG + chr2.1:59700203-59700222 MS.gene00086:intron 20.0%
!!! TATCTTGTTTATAGATGATA+AGG + chr2.1:59700959-59700978 MS.gene00086:intron 20.0%
!!! TTAATTTCCTTTTATGTTCA+CGG + chr2.1:59700167-59700186 MS.gene00086:intron 20.0%
! AAAAAGAAACTGTGATGTAA+TGG - chr2.1:59700894-59700913 None:intergenic 25.0%
! ACTATTGTCACATTAATGAT+TGG - chr2.1:59701756-59701775 None:intergenic 25.0%
! ATCTCCTACAGAAAAATAAA+GGG + chr2.1:59702808-59702827 MS.gene00086:CDS 25.0%
! TATAATTCCTTGATAAGCAA+TGG + chr2.1:59701159-59701178 MS.gene00086:CDS 25.0%
! TATCTCCTACAGAAAAATAA+AGG + chr2.1:59702807-59702826 MS.gene00086:CDS 25.0%
! TTATTTACCATTGCTTATCA+AGG - chr2.1:59701169-59701188 None:intergenic 25.0%
! TTGATCAAATGACTCTAAAT+GGG + chr2.1:59700633-59700652 MS.gene00086:CDS 25.0%
! TTTGAAGAAAAATGAAACTC+TGG + chr2.1:59702237-59702256 MS.gene00086:intron 25.0%
!! ATTGAACTTTTACCTTATGA+AGG + chr2.1:59702910-59702929 MS.gene00086:CDS 25.0%
!! CTTTTGATAAACAGTAGAAT+TGG - chr2.1:59702750-59702769 None:intergenic 25.0%
!! TGAATATTTTACTCACTGTA+GGG - chr2.1:59700858-59700877 None:intergenic 25.0%
!!! GAAGTTGTTTTTATGTTGTA+TGG + chr2.1:59702326-59702345 MS.gene00086:intron 25.0%
!!! TTGCCTTTTGAAATGATTTT+TGG - chr2.1:59701509-59701528 None:intergenic 25.0%
AAACTGTGATGTAATGGTTA+AGG - chr2.1:59700888-59700907 None:intergenic 30.0%
AACATTTGACAACATCCAAA+TGG + chr2.1:59702696-59702715 MS.gene00086:CDS 30.0%
ACATGTTCTAACATCTAAGA+AGG + chr2.1:59702203-59702222 MS.gene00086:intron 30.0%
AGTTTCTTACATTCTCCATT+TGG - chr2.1:59702714-59702733 None:intergenic 30.0%
ATAAAAGTTTGCAGCGTAAT+TGG + chr2.1:59700446-59700465 MS.gene00086:intron 30.0%
ATATATACACCCAAATTCTG+TGG + chr2.1:59701482-59701501 MS.gene00086:intron 30.0%
ATTATAGCTACATAGAAGCT+TGG - chr2.1:59702512-59702531 None:intergenic 30.0%
CAATTCCATGTGAAATTTGA+TGG + chr2.1:59701036-59701055 MS.gene00086:CDS 30.0%
CATAAGCATTCCAACAAAAA+GGG - chr2.1:59702951-59702970 None:intergenic 30.0%
CTTGATCAAATGACTCTAAA+TGG + chr2.1:59700632-59700651 MS.gene00086:CDS 30.0%
CTTGGTACTCAAAATTCTAT+AGG - chr2.1:59702494-59702513 None:intergenic 30.0%
GACTATAGAACTTTAACAGT+TGG + chr2.1:59701717-59701736 MS.gene00086:intron 30.0%
GGATTATTTGGATATGGATA+TGG - chr2.1:59699929-59699948 None:intergenic 30.0%
GGTCCAAAAATCATTTCAAA+AGG + chr2.1:59701503-59701522 MS.gene00086:intron 30.0%
GTCATAAAAGAATCAGACAA+TGG + chr2.1:59702625-59702644 MS.gene00086:CDS 30.0%
TAAGCTACAAACTTAGTTGA+TGG + chr2.1:59701448-59701467 MS.gene00086:intron 30.0%
TATCCATATCCAAATAATCC+TGG + chr2.1:59699929-59699948 MS.gene00086:CDS 30.0%
TCAAATTTCACATGGAATTG+AGG - chr2.1:59701036-59701055 None:intergenic 30.0%
TCAAGTAATTCACCTTCATA+AGG - chr2.1:59702925-59702944 None:intergenic 30.0%
TTACATATACTGCTGATGTT+TGG - chr2.1:59702783-59702802 None:intergenic 30.0%
! ATCACTTCTACTATCTTTTC+TGG + chr2.1:59700103-59700122 MS.gene00086:CDS 30.0%
! ATGGACTAAGAATCAAAACA+TGG + chr2.1:59702644-59702663 MS.gene00086:CDS 30.0%
! ATTCTTTTATGACTGAGTTG+CGG - chr2.1:59702618-59702637 None:intergenic 30.0%
! CTGAATATTTTACTCACTGT+AGG - chr2.1:59700859-59700878 None:intergenic 30.0%
! GATTCTTTTCATCCATAATG+TGG + chr2.1:59699799-59699818 MS.gene00086:CDS 30.0%
! GTGATCATTTGAAGTTAGAA+AGG - chr2.1:59701665-59701684 None:intergenic 30.0%
! TTTTCATTTCAGAGTCCAAA+TGG + chr2.1:59700805-59700824 MS.gene00086:intron 30.0%
!! ATTTTTGGACCACAGAATTT+GGG - chr2.1:59701494-59701513 None:intergenic 30.0%
!! TTTGGACTCTGAAATGAAAA+TGG - chr2.1:59700805-59700824 None:intergenic 30.0%
!! TTTTCTTTGGTTTCTTGTGA+TGG - chr2.1:59699835-59699854 None:intergenic 30.0%
!!! ATACCTTGGGACTAATTTTT+TGG + chr2.1:59701832-59701851 MS.gene00086:intron 30.0%
!!! TGTACCCTTTATTTTTCTGT+AGG - chr2.1:59702815-59702834 None:intergenic 30.0%
!!! TTACATGTGATGTGCTATTT+TGG + chr2.1:59702466-59702485 MS.gene00086:intron 30.0%
AAACTTAGTCCCAATACTGA+TGG + chr2.1:59701102-59701121 MS.gene00086:CDS 35.0%
AATACTGATGGAATCCACTT+AGG + chr2.1:59701114-59701133 MS.gene00086:CDS 35.0%
AATCCACTTAGGAAACACAA+GGG + chr2.1:59701125-59701144 MS.gene00086:CDS 35.0%
AATGGGACAGGAATTATTGA+AGG + chr2.1:59700650-59700669 MS.gene00086:CDS 35.0%
ACAGGAATTATTGAAGGCAA+TGG + chr2.1:59700656-59700675 MS.gene00086:CDS 35.0%
ACTAAGTTTAGGTGCAGAAA+TGG - chr2.1:59701091-59701110 None:intergenic 35.0%
AGGTAGCAATCTAATACCTT+GGG + chr2.1:59701819-59701838 MS.gene00086:intron 35.0%
AGTTATTACACTCACACTAC+AGG - chr2.1:59701554-59701573 None:intergenic 35.0%
CCAAATGGAAAAACTGTATC+AGG + chr2.1:59700820-59700839 MS.gene00086:CDS 35.0%
CCATAAGCATTCCAACAAAA+AGG - chr2.1:59702952-59702971 None:intergenic 35.0%
CTAAGAATCAAAACATGGCA+AGG + chr2.1:59702649-59702668 MS.gene00086:CDS 35.0%
GACAACCATCAAATTTCACA+TGG - chr2.1:59701044-59701063 None:intergenic 35.0%
GGTGATGATTGCATTTCAAT+TGG + chr2.1:59701280-59701299 MS.gene00086:intron 35.0%
GTCTATAAAACACAAGCCAT+TGG - chr2.1:59700613-59700632 None:intergenic 35.0%
GTGAGTGTAATAACTCTTGA+AGG + chr2.1:59701559-59701578 MS.gene00086:intron 35.0%
GTTCTTCATGAGCTCAAATT+TGG + chr2.1:59700981-59701000 MS.gene00086:CDS 35.0%
TAGGTAGCAATCTAATACCT+TGG + chr2.1:59701818-59701837 MS.gene00086:intron 35.0%
TCACTACAAGCAAAGTGTAT+TGG - chr2.1:59702856-59702875 None:intergenic 35.0%
TCCTCCACTTGATTGTTTAA+GGG + chr2.1:59702993-59703012 MS.gene00086:CDS 35.0%
TCTCCAGGATTATTTGGATA+TGG - chr2.1:59699935-59699954 None:intergenic 35.0%
TGTCCAAAAAATTAGTCCCA+AGG - chr2.1:59701838-59701857 None:intergenic 35.0%
TTCCTCCACTTGATTGTTTA+AGG + chr2.1:59702992-59703011 MS.gene00086:CDS 35.0%
! AAGGTATTAGATTGCTACCT+AGG - chr2.1:59701819-59701838 None:intergenic 35.0%
! AAGTGGATTCCATCAGTATT+GGG - chr2.1:59701114-59701133 None:intergenic 35.0%
! CCTGATACAGTTTTTCCATT+TGG - chr2.1:59700823-59700842 None:intergenic 35.0%
! CCTTTTTGTTGGAATGCTTA+TGG + chr2.1:59702949-59702968 MS.gene00086:CDS 35.0%
! CGCTGCAAACTTTTATATTG+CGG - chr2.1:59700442-59700461 None:intergenic 35.0%
! CTTTTCATCCATAATGTGGA+AGG + chr2.1:59699803-59699822 MS.gene00086:CDS 35.0%
! GTAGATTGATGGTACTTTGA+TGG + chr2.1:59700544-59700563 MS.gene00086:intron 35.0%
! GTGAAATTTGATGGTTGTCA+AGG + chr2.1:59701045-59701064 MS.gene00086:CDS 35.0%
! GTTTTCGATGTACGATCATT+CGG + chr2.1:59699968-59699987 MS.gene00086:CDS 35.0%
! TAAGTGGATTCCATCAGTAT+TGG - chr2.1:59701115-59701134 None:intergenic 35.0%
! TCAGTATTGGGACTAAGTTT+AGG - chr2.1:59701102-59701121 None:intergenic 35.0%
!! AGCTCAAATTTGGTGTTAAG+AGG + chr2.1:59700991-59701010 MS.gene00086:CDS 35.0%
!! GATTTTTGGACCACAGAATT+TGG - chr2.1:59701495-59701514 None:intergenic 35.0%
!! GCTCAAATTTGGTGTTAAGA+GGG + chr2.1:59700992-59701011 MS.gene00086:CDS 35.0%
!! TACTTGATGACCCTTTTTGT+TGG + chr2.1:59702938-59702957 MS.gene00086:CDS 35.0%
!!! ACGATCGTGGTTTTAAATTG+TGG + chr2.1:59700313-59700332 MS.gene00086:intron 35.0%
AACGCAACCGTGAACATAAA+AGG - chr2.1:59700177-59700196 None:intergenic 40.0%
ACATTCACATCTGAACATCC+AGG - chr2.1:59701307-59701326 None:intergenic 40.0%
AGAATCAAAACATGGCAAGG+AGG + chr2.1:59702652-59702671 MS.gene00086:CDS 40.0%
ATCTTCCTTGCAAACCTCAT+AGG + chr2.1:59700693-59700712 MS.gene00086:CDS 40.0%
ATGTGACATACCTAATTCCG+TGG - chr2.1:59701354-59701373 None:intergenic 40.0%
ATTACCCTATGAGGTTTGCA+AGG - chr2.1:59700701-59700720 None:intergenic 40.0%
CAAATGACTCTAAATGGGAC+AGG + chr2.1:59700638-59700657 MS.gene00086:CDS 40.0%
CCACTTGATTGTTTAAGGGA+AGG + chr2.1:59702997-59703016 MS.gene00086:CDS 40.0%
CCTTCCCTTAAACAATCAAG+TGG - chr2.1:59703000-59703019 None:intergenic 40.0%
CTACAAGCAAAGTGTATTGG+AGG - chr2.1:59702853-59702872 None:intergenic 40.0%
CTGTCGAAATCACATCAGTA+AGG - chr2.1:59702541-59702560 None:intergenic 40.0%
CTTACTTGAAATACCAGTCC+TGG - chr2.1:59700139-59700158 None:intergenic 40.0%
CTTCAAAATCCTTTACGCTG+CGG - chr2.1:59700358-59700377 None:intergenic 40.0%
GAATCCACTTAGGAAACACA+AGG + chr2.1:59701124-59701143 MS.gene00086:CDS 40.0%
GATAACTCCACAACTGTTTC+AGG - chr2.1:59703027-59703046 None:intergenic 40.0%
GATTGCATTTCAATTGGTCC+TGG + chr2.1:59701286-59701305 MS.gene00086:CDS 40.0%
GCACATCACATGTAACTTCT+AGG - chr2.1:59702461-59702480 None:intergenic 40.0%
GGAGATTCTCCAGGATTATT+TGG - chr2.1:59699941-59699960 None:intergenic 40.0%
GGATATGGATATGGATCAGA+GGG - chr2.1:59699920-59699939 None:intergenic 40.0%
GTGGTCTGCAAAAGTAATAG+AGG + chr2.1:59700332-59700351 MS.gene00086:intron 40.0%
TCATCCATAATGTGGAAGGA+AGG + chr2.1:59699807-59699826 MS.gene00086:CDS 40.0%
TCCCTTAAACAATCAAGTGG+AGG - chr2.1:59702997-59703016 None:intergenic 40.0%
TCTTCCTTGCAAACCTCATA+GGG + chr2.1:59700694-59700713 MS.gene00086:CDS 40.0%
TGATGTGATTTCGACAGCAT+TGG + chr2.1:59702544-59702563 MS.gene00086:intron 40.0%
TGGATATGGATATGGATCAG+AGG - chr2.1:59699921-59699940 None:intergenic 40.0%
TGGATGTTCAGATGTGAATG+TGG + chr2.1:59701306-59701325 MS.gene00086:CDS 40.0%
TGTGACATACCTAATTCCGT+GGG - chr2.1:59701353-59701372 None:intergenic 40.0%
TGTTCAGATGTGAATGTGGA+TGG + chr2.1:59701310-59701329 MS.gene00086:CDS 40.0%
TTAGACAAATGTGGCTGATG+TGG + chr2.1:59700212-59700231 MS.gene00086:intron 40.0%
TTAGGAAACACAAGGGATGT+TGG + chr2.1:59701132-59701151 MS.gene00086:CDS 40.0%
TTGAAGGCAATGGAGAACAA+TGG + chr2.1:59700666-59700685 MS.gene00086:CDS 40.0%
! CTGCAGAAATTTTTACGCTG+TGG + chr2.1:59700256-59700275 MS.gene00086:intron 40.0%
! GCAATTTAGAATCACGATCG+TGG + chr2.1:59700300-59700319 MS.gene00086:intron 40.0%
AAGGCAATGGAGAACAATGG+TGG + chr2.1:59700669-59700688 MS.gene00086:CDS 45.0%
ACATACCTAATTCCGTGGGT+AGG - chr2.1:59701349-59701368 None:intergenic 45.0%
ACTAGGAGATGTAGGAGGAT+TGG - chr2.1:59699894-59699913 None:intergenic 45.0%
AGGCAATGGAGAACAATGGT+GGG + chr2.1:59700670-59700689 MS.gene00086:CDS 45.0%
AGGCAGACAGTAAAAGCCAA+TGG + chr2.1:59700594-59700613 MS.gene00086:CDS 45.0%
CACCAGATGGACCAAATTGT+TGG + chr2.1:59700567-59700586 MS.gene00086:CDS 45.0%
CATCCCTTGTGTTTCCTAAG+TGG - chr2.1:59701131-59701150 None:intergenic 45.0%
CGTACATCGAAAACGCTTGA+AGG - chr2.1:59699962-59699981 None:intergenic 45.0%
GATGTAGGAGGATTGGAAGA+AGG - chr2.1:59699887-59699906 None:intergenic 45.0%
GATTGGAAGAAGGTGGTGAA+GGG - chr2.1:59699877-59699896 None:intergenic 45.0%
GGCTGCAATTATGGTTGCAA+AGG + chr2.1:59700233-59700252 MS.gene00086:intron 45.0%
GGTACCTTCCTTCCACATTA+TGG - chr2.1:59699814-59699833 None:intergenic 45.0%
TAGATTGCTACCTAGGAGCA+GGG - chr2.1:59701812-59701831 None:intergenic 45.0%
TATGGATCAGAGGGAACACT+AGG - chr2.1:59699911-59699930 None:intergenic 45.0%
TTAGATTGCTACCTAGGAGC+AGG - chr2.1:59701813-59701832 None:intergenic 45.0%
! AAAACATGGCAAGGAGGTAC+AGG + chr2.1:59702658-59702677 MS.gene00086:CDS 45.0%
! AATACCAGTCCTGGTTTGCA+TGG - chr2.1:59700130-59700149 None:intergenic 45.0%
!! TTTTCTGGTCCATGCAAACC+AGG + chr2.1:59700118-59700137 MS.gene00086:CDS 45.0%
!!! AATTTTTTTTTTTTTTTTGA+AGG + chr2.1:59702082-59702101 MS.gene00086:intron 5.0%
!!! TTTTTTCTTTAAATTTATTT+TGG + chr2.1:59702415-59702434 MS.gene00086:intron 5.0%
ACGCTTGAAGGAGATTCTCC+AGG - chr2.1:59699950-59699969 None:intergenic 50.0%
AGATTGCTACCTAGGAGCAG+GGG - chr2.1:59701811-59701830 None:intergenic 50.0%
AGGTGAGCCTGAAACAGTTG+TGG + chr2.1:59703017-59703036 MS.gene00086:CDS 50.0%
CTCACTGTAGGGCTAACACA+TGG - chr2.1:59700847-59700866 None:intergenic 50.0%
GAGGGAACACTAGGAGATGT+AGG - chr2.1:59699902-59699921 None:intergenic 50.0%
GGAACACTAGGAGATGTAGG+AGG - chr2.1:59699899-59699918 None:intergenic 50.0%
GGATTGGAAGAAGGTGGTGA+AGG - chr2.1:59699878-59699897 None:intergenic 50.0%
GGCCAACAATTTGGTCCATC+TGG - chr2.1:59700572-59700591 None:intergenic 50.0%
GGCTGATGTGGCTGCAATTA+TGG + chr2.1:59700224-59700243 MS.gene00086:intron 50.0%
GTACGATCATTCGGAGCAGT+TGG + chr2.1:59699977-59699996 MS.gene00086:CDS 50.0%
GTAGGAGGATTGGAAGAAGG+TGG - chr2.1:59699884-59699903 None:intergenic 50.0%
GTTACTTGTGCTCCTACCCA+CGG + chr2.1:59701334-59701353 MS.gene00086:CDS 50.0%
TAATAGAGGCCGCAGCGTAA+AGG + chr2.1:59700346-59700365 MS.gene00086:intron 50.0%
TAGGTTCGAGACACATGCGT+GGG - chr2.1:59702590-59702609 None:intergenic 50.0%
TCATTCGGAGCAGTTGGAGA+TGG + chr2.1:59699983-59700002 MS.gene00086:CDS 50.0%
TCTGCCTCAGGCCAACAATT+TGG - chr2.1:59700581-59700600 None:intergenic 50.0%
TTAGGTTCGAGACACATGCG+TGG - chr2.1:59702591-59702610 None:intergenic 50.0%
! CAGCTGCATTTAGAGCAGCA+TGG + chr2.1:59700020-59700039 MS.gene00086:CDS 50.0%
! GCAGCTTGTGCAGTTGATTC+AGG + chr2.1:59700046-59700065 MS.gene00086:CDS 50.0%
! GGAGGTACAGGATCAGTAAC+AGG + chr2.1:59702670-59702689 MS.gene00086:CDS 50.0%
! GGTACTTTGATGGCACCAGA+TGG + chr2.1:59700554-59700573 MS.gene00086:CDS 50.0%
! TGACTGAGTTGCGGACTGTT+AGG - chr2.1:59702609-59702628 None:intergenic 50.0%
! TGGACCAAATTGTTGGCCTG+AGG + chr2.1:59700574-59700593 MS.gene00086:CDS 50.0%
! TGGCTTTTACTGTCTGCCTC+AGG - chr2.1:59700593-59700612 None:intergenic 50.0%
! TTCGACAGCATTGGAAGCCT+TGG + chr2.1:59702553-59702572 MS.gene00086:intron 50.0%
!! GGTGCTGGGGAAGTTTTCTT+TGG - chr2.1:59699848-59699867 None:intergenic 50.0%
CGTGGGAGTTGTGTACTCCA+AGG - chr2.1:59702573-59702592 None:intergenic 55.0%
GAAGGTGGTGAAGGGTCACT+AGG - chr2.1:59699869-59699888 None:intergenic 55.0%
GTGAAGGGTCACTAGGTGCT+GGG - chr2.1:59699862-59699881 None:intergenic 55.0%
GTGCTCCTACCCACGGAATT+AGG + chr2.1:59701341-59701360 MS.gene00086:CDS 55.0%
TGGTCCATGCAAACCAGGAC+TGG + chr2.1:59700123-59700142 MS.gene00086:CDS 55.0%
! CTGTTTTGTCCCCTGCTCCT+AGG + chr2.1:59701799-59701818 MS.gene00086:intron 55.0%
! TGAAGGGTCACTAGGTGCTG+GGG - chr2.1:59699861-59699880 None:intergenic 55.0%
GGTGAAGGGTCACTAGGTGC+TGG - chr2.1:59699863-59699882 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 59699797 59703068 59699797 ID=MS.gene00086
chr2.1 mRNA 59699797 59703068 59699797 ID=MS.gene00086.t1;Parent=MS.gene00086
chr2.1 exon 59699797 59700153 59699797 ID=MS.gene00086.t1.exon1;Parent=MS.gene00086.t1
chr2.1 CDS 59699797 59700153 59699797 ID=cds.MS.gene00086.t1;Parent=MS.gene00086.t1
chr2.1 exon 59700548 59700715 59700548 ID=MS.gene00086.t1.exon2;Parent=MS.gene00086.t1
chr2.1 CDS 59700548 59700715 59700548 ID=cds.MS.gene00086.t1;Parent=MS.gene00086.t1
chr2.1 exon 59700817 59700861 59700817 ID=MS.gene00086.t1.exon3;Parent=MS.gene00086.t1
chr2.1 CDS 59700817 59700861 59700817 ID=cds.MS.gene00086.t1;Parent=MS.gene00086.t1
chr2.1 exon 59700973 59701180 59700973 ID=MS.gene00086.t1.exon4;Parent=MS.gene00086.t1
chr2.1 CDS 59700973 59701180 59700973 ID=cds.MS.gene00086.t1;Parent=MS.gene00086.t1
chr2.1 exon 59701281 59701362 59701281 ID=MS.gene00086.t1.exon5;Parent=MS.gene00086.t1
chr2.1 CDS 59701281 59701362 59701281 ID=cds.MS.gene00086.t1;Parent=MS.gene00086.t1
chr2.1 exon 59702561 59703068 59702561 ID=MS.gene00086.t1.exon6;Parent=MS.gene00086.t1
chr2.1 CDS 59702561 59703068 59702561 ID=cds.MS.gene00086.t1;Parent=MS.gene00086.t1
Gene Sequence

>MS.gene00086

ATGATTCTTTTCATCCATAATGTGGAAGGAAGGTACCATCACAAGAAACCAAAGAAAACTTCCCCAGCACCTAGTGACCCTTCACCACCTTCTTCCAATCCTCCTACATCTCCTAGTGTTCCCTCTGATCCATATCCATATCCAAATAATCCTGGAGAATCTCCTTCAAGCGTTTTCGATGTACGATCATTCGGAGCAGTTGGAGATGGTGATGCTGATGATACAGCTGCATTTAGAGCAGCATGGAAAGCAGCTTGTGCAGTTGATTCAGGAGTTCTTTTAGCTCCTAAAAATTACTGTTTTAAAATCACTTCTACTATCTTTTCTGGTCCATGCAAACCAGGACTGGTATTTCAAGTAAGAATGTTTCTTAATTTCCTTTTATGTTCACGGTTGCGTTGCGATTCTTTAAATTTTAGACAAATGTGGCTGATGTGGCTGCAATTATGGTTGCAAAGGCTGCAGAAATTTTTACGCTGTGGTCGCAATTGCTGTTATAAACTGCAATTTAGAATCACGATCGTGGTTTTAAATTGTGGTCTGCAAAAGTAATAGAGGCCGCAGCGTAAAGGATTTTGAAGTCTCTTCAAAGTCATTGTAACTATAATTTTGACTGCATCAGCTGCATTTTCTCTAATTTTTCCGCAATATAAAAGTTTGCAGCGTAATTGGAACTGAAATTTAACATGACCATCATTGTGTAAAATTTAATTTCCTATATAATTAACTAACAAGGTTGAAATATATGTAGATTGATGGTACTTTGATGGCACCAGATGGACCAAATTGTTGGCCTGAGGCAGACAGTAAAAGCCAATGGCTTGTGTTTTATAGACTTGATCAAATGACTCTAAATGGGACAGGAATTATTGAAGGCAATGGAGAACAATGGTGGGATCTTCCTTGCAAACCTCATAGGGTAATAATAATAATAATAATTAACATTAACACCATTATTTTTGTATTTATCTATCTAATGTTAAAAACTAAAATTCTGGTAATTTTCCATTTTCATTTCAGAGTCCAAATGGAAAAACTGTATCAGGACCATGTGTTAGCCCTACAGTGAGTAAAATATTCAGAATTTACCTTAACCATTACATCACAGTTTCTTTTTTTTTTTTTAAATATGAAATGCCTTATAATTTTTTTTCTTATGAAATATCTTGTTTATAGATGATAAGGTTCTTCATGAGCTCAAATTTGGTGTTAAGAGGGTTGAAAATACAAAATAGTCCTCAATTCCATGTGAAATTTGATGGTTGTCAAGGAGTATTAATTGATGAATTGTCCATTTCTGCACCTAAACTTAGTCCCAATACTGATGGAATCCACTTAGGAAACACAAGGGATGTTGGAATATATAATTCCTTGATAAGCAATGGTAAATAATAAAGATCATTATCACACTTTCTCAATATCGAAATTTTATTCTATGGTTTTTTATTTAATTATGTCACATAATGTTACATTTTATTTAATAGGTGATGATTGCATTTCAATTGGTCCTGGATGTTCAGATGTGAATGTGGATGGTGTTACTTGTGCTCCTACCCACGGAATTAGGTATGTCACATAGTTTCTGTTATTTTAGTTAAATTGGGTTGTTAACTATCAATTAATTATGATTAATTTAGGAATAAAATAAAAATAAGCTACAAACTTAGTTGATGGTATTCATATAAATATATACACCCAAATTCTGTGGTCCAAAAATCATTTCAAAAGGCAAAATGAATGAAAAGTGACTTTAACCTGTAGTGTGAGTGTAATAACTCTTGAAGGTGTCTTATAAGATAGCAATTTATCTTCAAGAAAAATAAATAAATAAATAAATAATGGAACATTTTTGTTTGAAATAGAAACCTTTCTAACTTCAAATGATCACAAGATTAATTTTCTTCTTTTAGAGATTTATGTGACTATAGAACTTTAACAGTTGGAAGTTTCAAACTGCCAATCATTAATGTGACAATAGTTTTTGTTCTTCATTTCTGTAATTCTGTTTTGTCCCCTGCTCCTAGGTAGCAATCTAATACCTTGGGACTAATTTTTTGGACATGACTTTTAAAGATGTTGATTATGTAAAACTTAATAACATAAAATTGAGTTTGAAAAAAAAAAGTGATTTAAATTCATATGAATTTATTTTGAAATCAAATTTGAATTTTTTAGAGGTACAAAACTTATTTTATATGTTTGGATGCTATAGAGTATAATTGATTTTGTCTCAAAAATTAACTCTAACTTAGCTAGAATTTTGTGCTCTAAAGTCTAAACTCTACAATTTTTTTTTTTTTTTTGAAGGAGTCTAAACTCTACAATTGTTTTTAAGATTAAATTTTTTTCAATTCACTTTGACATTCAACACACTACTTGATGAGAAGCACACACTTAATAGACTAGACATGTTCTAACATCTAAGAAGGACCAAAATAAATTTGAAGAAAAATGAAACTCTGGTAACTCGTTGAACATTTACAATTTTTCATAAAATAATTACATAATTGCAATCAGTGTCATAGATTAGAAGTTGTTTTTATGTTGTATGGCTTGATTGCAATTATGTAATTATTTTATGTTGTATGGCTTGATTGTAAATGTTCAGCAGAGTTCTTTTTTTTCTTTAAATTTATTTTGGTCTCTAGTATGAGACATCTTCCTAGAAGTTACATGTGATGTGCTATTTTGGAACCTATAGAATTTTGAGTACCAAGCTTCTATGTAGCTATAATTAAAAACCTTACTGATGTGATTTCGACAGCATTGGAAGCCTTGGAGTACACAACTCCCACGCATGTGTCTCGAACCTAACAGTCCGCAACTCAGTCATAAAAGAATCAGACAATGGACTAAGAATCAAAACATGGCAAGGAGGTACAGGATCAGTAACAGGTTTAACATTTGACAACATCCAAATGGAGAATGTAAGAAACTGTATCAACATAGACCAATTCTACTGTTTATCAAAAGAGTGTATGAACCAAACATCAGCAGTATATGTAAACAATATCTCCTACAGAAAAATAAAGGGTACATATGATGTGAGAACACCTCCAATACACTTTGCTTGTAGTGACACTGTTGCTTGCACAAACATCACACTCTCTGAGATTGAACTTTTACCTTATGAAGGTGAATTACTTGATGACCCTTTTTGTTGGAATGCTTATGGAAGACAAGAGACTTTGACTATTCCTCCACTTGATTGTTTAAGGGAAGGTGAGCCTGAAACAGTTGTGGAGTTATCTGAATATGAATGCAATAATTAA
Protein sequence

>MS.gene00086.t1

MILFIHNVEGRYHHKKPKKTSPAPSDPSPPSSNPPTSPSVPSDPYPYPNNPGESPSSVFDVRSFGAVGDGDADDTAAFRAAWKAACAVDSGVLLAPKNYCFKITSTIFSGPCKPGLVFQIDGTLMAPDGPNCWPEADSKSQWLVFYRLDQMTLNGTGIIEGNGEQWWDLPCKPHRSPNGKTVSGPCVSPTMIRFFMSSNLVLRGLKIQNSPQFHVKFDGCQGVLIDELSISAPKLSPNTDGIHLGNTRDVGIYNSLISNGDDCISIGPGCSDVNVDGVTCAPTHGISIGSLGVHNSHACVSNLTVRNSVIKESDNGLRIKTWQGGTGSVTGLTFDNIQMENVRNCINIDQFYCLSKECMNQTSAVYVNNISYRKIKGTYDVRTPPIHFACSDTVACTNITLSEIELLPYEGELLDDPFCWNAYGRQETLTIPPLDCLREGEPETVVELSEYECNN