Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001083.t1 | XP_013442640.1 | 70.6 | 218 | 29 | 1 | 1 | 183 | 1 | 218 | 2.80E-74 | 288.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001083.t1 | Q9FKK2 | 32.0 | 228 | 105 | 6 | 5 | 182 | 3 | 230 | 1.0e-16 | 88.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001083.t1 | A0A072TSY1 | 70.6 | 218 | 29 | 1 | 1 | 183 | 1 | 218 | 2.0e-74 | 288.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene001083 | MS.gene22094 | PPI |
| MS.gene001083 | MS.gene068836 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001083.t1 | MTR_0121s0080 | 70.642 | 218 | 29 | 1 | 1 | 183 | 1 | 218 | 7.77e-104 | 298 |
| MS.gene001083.t1 | MTR_7g062350 | 83.648 | 159 | 26 | 0 | 25 | 183 | 72 | 230 | 2.04e-92 | 270 |
| MS.gene001083.t1 | MTR_8g043650 | 59.174 | 218 | 54 | 1 | 1 | 183 | 1 | 218 | 8.52e-83 | 244 |
| MS.gene001083.t1 | MTR_2g030740 | 57.339 | 218 | 58 | 1 | 1 | 183 | 1 | 218 | 1.02e-80 | 239 |
| MS.gene001083.t1 | MTR_1g105920 | 58.257 | 218 | 56 | 1 | 1 | 183 | 1 | 218 | 3.22e-80 | 238 |
| MS.gene001083.t1 | MTR_1g012570 | 57.339 | 218 | 58 | 1 | 1 | 183 | 1 | 218 | 1.80e-77 | 231 |
| MS.gene001083.t1 | MTR_1g105910 | 56.422 | 218 | 60 | 1 | 1 | 183 | 1 | 218 | 5.25e-77 | 230 |
| MS.gene001083.t1 | MTR_3g052920 | 54.587 | 218 | 64 | 1 | 1 | 183 | 1 | 218 | 9.04e-74 | 222 |
| MS.gene001083.t1 | MTR_2g093190 | 57.273 | 220 | 49 | 3 | 1 | 183 | 1 | 212 | 1.07e-72 | 219 |
| MS.gene001083.t1 | MTR_1g108580 | 56.481 | 216 | 51 | 2 | 1 | 181 | 1 | 208 | 1.75e-72 | 218 |
| MS.gene001083.t1 | MTR_1g108510 | 55.455 | 220 | 53 | 3 | 1 | 183 | 1 | 212 | 6.29e-72 | 217 |
| MS.gene001083.t1 | MTR_3g030770 | 55.909 | 220 | 52 | 3 | 1 | 183 | 1 | 212 | 2.41e-70 | 213 |
| MS.gene001083.t1 | MTR_1g077360 | 48.624 | 218 | 77 | 1 | 1 | 183 | 1 | 218 | 1.38e-62 | 193 |
| MS.gene001083.t1 | MTR_1g075600 | 49.083 | 218 | 76 | 1 | 1 | 183 | 1 | 218 | 1.68e-62 | 193 |
| MS.gene001083.t1 | MTR_1g105905 | 59.119 | 159 | 65 | 0 | 25 | 183 | 43 | 201 | 1.55e-61 | 190 |
| MS.gene001083.t1 | MTR_5g053390 | 48.165 | 218 | 78 | 1 | 1 | 183 | 1 | 218 | 4.63e-60 | 187 |
| MS.gene001083.t1 | MTR_3g052870 | 50.459 | 218 | 54 | 3 | 1 | 183 | 1 | 199 | 1.13e-59 | 185 |
| MS.gene001083.t1 | MTR_2g085250 | 47.248 | 218 | 80 | 1 | 1 | 183 | 1 | 218 | 2.53e-59 | 185 |
| MS.gene001083.t1 | MTR_2g085280 | 46.789 | 218 | 81 | 1 | 1 | 183 | 1 | 218 | 1.25e-58 | 183 |
| MS.gene001083.t1 | MTR_1g106070 | 55.307 | 179 | 45 | 1 | 1 | 144 | 1 | 179 | 5.02e-58 | 180 |
| MS.gene001083.t1 | MTR_5g055100 | 46.330 | 218 | 82 | 1 | 1 | 183 | 1 | 218 | 1.08e-56 | 178 |
| MS.gene001083.t1 | MTR_5g045560 | 46.789 | 218 | 81 | 1 | 1 | 183 | 1 | 218 | 4.92e-56 | 177 |
| MS.gene001083.t1 | MTR_3g030780 | 62.879 | 132 | 14 | 1 | 1 | 97 | 1 | 132 | 3.23e-47 | 151 |
| MS.gene001083.t1 | MTR_0121s0100 | 52.273 | 132 | 28 | 1 | 1 | 97 | 1 | 132 | 2.88e-33 | 115 |
| MS.gene001083.t1 | MTR_1g075570 | 45.732 | 164 | 68 | 2 | 18 | 160 | 1 | 164 | 8.39e-30 | 107 |
| MS.gene001083.t1 | MTR_3g067915 | 60.294 | 68 | 27 | 0 | 24 | 91 | 29 | 96 | 2.87e-22 | 86.3 |
| MS.gene001083.t1 | MTR_3g080940 | 33.099 | 142 | 89 | 1 | 25 | 160 | 60 | 201 | 9.49e-21 | 85.5 |
| MS.gene001083.t1 | MTR_3g067910 | 54.688 | 64 | 29 | 0 | 25 | 88 | 41 | 104 | 2.35e-17 | 73.9 |
| MS.gene001083.t1 | MTR_1g108500 | 57.627 | 59 | 4 | 1 | 25 | 83 | 148 | 185 | 2.87e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001083.t1 | AT5G60440 | 36.310 | 168 | 69 | 3 | 5 | 134 | 3 | 170 | 8.80e-22 | 90.1 |
Find 32 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTGATAGGATTGCAATTC+AGG | 0.214281 | 3.4:+24204495 | MS.gene001083:CDS |
| TGAATTATTCAAGAAGATTT+TGG | 0.235888 | 3.4:+24204444 | MS.gene001083:CDS |
| GTCATAGATCGCTATCTCTC+TGG | 0.302606 | 3.4:+24204214 | MS.gene001083:CDS |
| TGGTGGGCTTGTCTGATTAA+TGG | 0.313412 | 3.4:+24204403 | MS.gene001083:CDS |
| TCTGATTAATGGGATGAATA+AGG | 0.315380 | 3.4:+24204414 | MS.gene001083:CDS |
| GGTGGGCTTGTCTGATTAAT+GGG | 0.325502 | 3.4:+24204404 | MS.gene001083:CDS |
| AGGCGTTCAAGGCTCAATTT+TGG | 0.334747 | 3.4:+24204383 | MS.gene001083:CDS |
| GTTGAGGATTTCGCTGATAA+TGG | 0.359317 | 3.4:-24204579 | None:intergenic |
| GCGAGCCTCAATAAATTCTA+TGG | 0.364151 | 3.4:-24204260 | None:intergenic |
| CCTTGAACGCCTTGCACAAA+AGG | 0.373825 | 3.4:-24204372 | None:intergenic |
| TTGTTGCTCATCATGCTGAT+AGG | 0.400809 | 3.4:+24204482 | MS.gene001083:CDS |
| TCATAGATCGCTATCTCTCT+GGG | 0.423793 | 3.4:+24204215 | MS.gene001083:CDS |
| TCTATGACCGTATCAACATT+TGG | 0.448129 | 3.4:-24204199 | None:intergenic |
| ATAATGGAGAGGGATGTTAG+AGG | 0.448969 | 3.4:-24204563 | None:intergenic |
| ATTTAATGAGGTTGCAACAT+AGG | 0.459732 | 3.4:-24204637 | None:intergenic |
| AGCATTGAGCTCACACACTT+TGG | 0.483043 | 3.4:-24204290 | None:intergenic |
| TTTAATGAGGTTGCAACATA+GGG | 0.485784 | 3.4:-24204636 | None:intergenic |
| ACCTTTCCTTCTGCTTGACA+TGG | 0.501316 | 3.4:-24204022 | None:intergenic |
| GCTCACACACTTTGGCGCTA+TGG | 0.507496 | 3.4:-24204282 | None:intergenic |
| CGGTCATCCAAATGTTGATA+CGG | 0.514501 | 3.4:+24204192 | MS.gene001083:CDS |
| GCTGATAGGATTGCAATTCA+GGG | 0.533534 | 3.4:+24204496 | MS.gene001083:CDS |
| TTGTTGAAGCCAATTTAATG+AGG | 0.541384 | 3.4:-24204649 | None:intergenic |
| GAAAACATCTCAGTTTGTTG+AGG | 0.554882 | 3.4:-24204595 | None:intergenic |
| TAGCACCATAGAATTTATTG+AGG | 0.563308 | 3.4:+24204255 | MS.gene001083:CDS |
| TCAAGCAGAAGGAAAGGTCG+TGG | 0.571573 | 3.4:+24204027 | MS.gene001083:CDS |
| CTTCAACCATGTCAAGCAGA+AGG | 0.615319 | 3.4:+24204016 | None:intergenic |
| ACCATGTCAAGCAGAAGGAA+AGG | 0.615544 | 3.4:+24204021 | None:intergenic |
| CAAGCAGAAGGAAAGGTCGT+GGG | 0.623500 | 3.4:+24204028 | MS.gene001083:CDS |
| GATTTCGCTGATAATGGAGA+GGG | 0.639954 | 3.4:-24204573 | None:intergenic |
| GGATTTCGCTGATAATGGAG+AGG | 0.643251 | 3.4:-24204574 | None:intergenic |
| AATCAACAACACACTAGACG+AGG | 0.684390 | 3.4:+24204324 | MS.gene001083:CDS |
| CATAGATCGCTATCTCTCTG+GGG | 0.721259 | 3.4:+24204216 | MS.gene001083:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGAATTATTCAAGAAGATTT+TGG | + | chr3.4:24204444-24204463 | MS.gene001083:CDS | 20.0% |
| !!! | TTTTTTTCATCTCAATCTTT+TGG | - | chr3.4:24204056-24204075 | None:intergenic | 20.0% |
| ! | ATAGGGTTATGAAAAATTTG+TGG | - | chr3.4:24204622-24204641 | None:intergenic | 25.0% |
| !! | AAAGAAAATACCTTTTCACT+AGG | - | chr3.4:24204172-24204191 | None:intergenic | 25.0% |
| !! | ATTATTCAAGAAGATTTTGG+TGG | + | chr3.4:24204447-24204466 | MS.gene001083:CDS | 25.0% |
| !!! | AGTGAAAAGGTATTTTCTTT+CGG | + | chr3.4:24204172-24204191 | MS.gene001083:intron | 25.0% |
| TAGCACCATAGAATTTATTG+AGG | + | chr3.4:24204255-24204274 | MS.gene001083:CDS | 30.0% | |
| TCTGATTAATGGGATGAATA+AGG | + | chr3.4:24204414-24204433 | MS.gene001083:CDS | 30.0% | |
| ! | CAAACTTTTCGATTTTCTGT+TGG | + | chr3.4:24204529-24204548 | MS.gene001083:CDS | 30.0% |
| TCTATGACCGTATCAACATT+TGG | - | chr3.4:24204202-24204221 | None:intergenic | 35.0% | |
| TGTATTCTCACCTAGTGAAA+AGG | + | chr3.4:24204159-24204178 | MS.gene001083:intron | 35.0% | |
| !! | GAAAACATCTCAGTTTGTTG+AGG | - | chr3.4:24204598-24204617 | None:intergenic | 35.0% |
| !!! | TCTATGGTGCTATTGTTTTG+CGG | - | chr3.4:24204247-24204266 | None:intergenic | 35.0% |
| AATCAACAACACACTAGACG+AGG | + | chr3.4:24204324-24204343 | MS.gene001083:CDS | 40.0% | |
| CGGTCATCCAAATGTTGATA+CGG | + | chr3.4:24204192-24204211 | MS.gene001083:CDS | 40.0% | |
| GCGAGCCTCAATAAATTCTA+TGG | - | chr3.4:24204263-24204282 | None:intergenic | 40.0% | |
| GCTGATAGGATTGCAATTCA+GGG | + | chr3.4:24204496-24204515 | MS.gene001083:CDS | 40.0% | |
| GTTGAGGATTTCGCTGATAA+TGG | - | chr3.4:24204582-24204601 | None:intergenic | 40.0% | |
| TCATAGATCGCTATCTCTCT+GGG | + | chr3.4:24204215-24204234 | MS.gene001083:CDS | 40.0% | |
| TGCTGATAGGATTGCAATTC+AGG | + | chr3.4:24204495-24204514 | MS.gene001083:CDS | 40.0% | |
| TTGTTGCTCATCATGCTGAT+AGG | + | chr3.4:24204482-24204501 | MS.gene001083:CDS | 40.0% | |
| ! | ATAATGGAGAGGGATGTTAG+AGG | - | chr3.4:24204566-24204585 | None:intergenic | 40.0% |
| !! | GATTTCGCTGATAATGGAGA+GGG | - | chr3.4:24204576-24204595 | None:intergenic | 40.0% |
| !! | GTTCAAGGCTCAATTTTGGT+GGG | + | chr3.4:24204387-24204406 | MS.gene001083:CDS | 40.0% |
| CATAGATCGCTATCTCTCTG+GGG | + | chr3.4:24204216-24204235 | MS.gene001083:CDS | 45.0% | |
| CTAGACGAGGAGAAAAAGCA+TGG | + | chr3.4:24204337-24204356 | MS.gene001083:CDS | 45.0% | |
| GTCATAGATCGCTATCTCTC+TGG | + | chr3.4:24204214-24204233 | MS.gene001083:CDS | 45.0% | |
| ! | AGCATTGAGCTCACACACTT+TGG | - | chr3.4:24204293-24204312 | None:intergenic | 45.0% |
| ! | AGGCGTTCAAGGCTCAATTT+TGG | + | chr3.4:24204383-24204402 | MS.gene001083:CDS | 45.0% |
| ! | CGTTCAAGGCTCAATTTTGG+TGG | + | chr3.4:24204386-24204405 | MS.gene001083:CDS | 45.0% |
| ! | GGTGGGCTTGTCTGATTAAT+GGG | + | chr3.4:24204404-24204423 | MS.gene001083:CDS | 45.0% |
| !! | ACTTTCTCAAAGCGTCGTAG+CGG | + | chr3.4:24204102-24204121 | MS.gene001083:intron | 45.0% |
| !! | GACGCTTTGAGAAAGTCACT+TGG | - | chr3.4:24204098-24204117 | None:intergenic | 45.0% |
| !! | GCTTTGAGAAAGTCACTTGG+AGG | - | chr3.4:24204095-24204114 | None:intergenic | 45.0% |
| !! | GGATTTCGCTGATAATGGAG+AGG | - | chr3.4:24204577-24204596 | None:intergenic | 45.0% |
| !! | TGAGTTGAGCCTTTTGTGCA+AGG | + | chr3.4:24204363-24204382 | MS.gene001083:CDS | 45.0% |
| !! | TGGTGGGCTTGTCTGATTAA+TGG | + | chr3.4:24204403-24204422 | MS.gene001083:CDS | 45.0% |
| !!! | ATGGTGCTATTGTTTTGCGG+TGG | - | chr3.4:24204244-24204263 | None:intergenic | 45.0% |
| !!! | TGGTGCTATTGTTTTGCGGT+GGG | - | chr3.4:24204243-24204262 | None:intergenic | 45.0% |
| CAAGCAGAAGGAAAGGTCGT+GGG | + | chr3.4:24204028-24204047 | MS.gene001083:CDS | 50.0% | |
| CCTTGAACGCCTTGCACAAA+AGG | - | chr3.4:24204375-24204394 | None:intergenic | 50.0% | |
| TCAAGCAGAAGGAAAGGTCG+TGG | + | chr3.4:24204027-24204046 | MS.gene001083:CDS | 50.0% | |
| !! | CCTTTTGTGCAAGGCGTTCA+AGG | + | chr3.4:24204372-24204391 | MS.gene001083:CDS | 50.0% |
| !! | CTTTCTCAAAGCGTCGTAGC+GGG | + | chr3.4:24204103-24204122 | MS.gene001083:intron | 50.0% |
| GCTCACACACTTTGGCGCTA+TGG | - | chr3.4:24204285-24204304 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 24204024 | 24204657 | 24204024 | ID=MS.gene001083 |
| chr3.4 | mRNA | 24204024 | 24204657 | 24204024 | ID=MS.gene001083.t1;Parent=MS.gene001083 |
| chr3.4 | exon | 24204024 | 24204098 | 24204024 | ID=MS.gene001083.t1.exon1;Parent=MS.gene001083.t1 |
| chr3.4 | CDS | 24204024 | 24204098 | 24204024 | ID=cds.MS.gene001083.t1;Parent=MS.gene001083.t1 |
| chr3.4 | exon | 24204181 | 24204657 | 24204181 | ID=MS.gene001083.t1.exon2;Parent=MS.gene001083.t1 |
| chr3.4 | CDS | 24204181 | 24204657 | 24204181 | ID=cds.MS.gene001083.t1;Parent=MS.gene001083.t1 |
| Gene Sequence |
| Protein sequence |
