AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene007670

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007670.t1 MTR_4g058930 98.711 698 8 1 1 697 1 698 0.0 1426
MS.gene007670.t1 MTR_4g058930 98.661 672 8 1 1 671 1 672 0.0 1370
MS.gene007670.t1 MTR_2g094570 70.759 725 158 20 1 697 57 755 0.0 976
MS.gene007670.t1 MTR_2g094570 70.759 725 158 20 1 697 1 699 0.0 975
MS.gene007670.t1 MTR_2g094570 70.398 679 147 20 1 651 1 653 0.0 905
MS.gene007670.t1 MTR_1g064430 58.874 693 219 14 9 664 37 700 0.0 764
MS.gene007670.t1 MTR_1g064430 58.874 693 219 14 9 664 24 687 0.0 764
MS.gene007670.t1 MTR_1g094960 58.957 575 204 14 1 554 8 571 0.0 596
MS.gene007670.t1 MTR_7g101280 55.453 541 214 12 9 536 28 554 0.0 571
MS.gene007670.t1 MTR_0041s0110 55.453 541 214 12 9 536 28 554 0.0 571
MS.gene007670.t1 MTR_3g073420 42.549 557 295 13 9 558 16 554 1.54e-122 378
MS.gene007670.t1 MTR_7g101275 51.491 369 126 7 21 385 1 320 7.29e-119 362
MS.gene007670.t1 MTR_0041s0120 50.400 375 133 8 21 385 1 332 6.32e-117 357
MS.gene007670.t1 MTR_5g061220 41.079 482 221 14 12 474 19 456 7.29e-99 314
MS.gene007670.t1 MTR_2g024430 41.119 411 157 11 9 402 17 359 3.57e-89 288
MS.gene007670.t1 MTR_5g040880 37.630 481 212 12 4 458 3 421 8.52e-87 282
MS.gene007670.t1 MTR_5g082140 39.062 448 189 12 1 429 1 383 8.50e-84 272
MS.gene007670.t1 MTR_2g018690 38.926 447 205 10 2 436 13 403 5.50e-83 281
MS.gene007670.t1 MTR_8g079492 39.013 446 205 10 2 436 13 402 1.77e-82 280
MS.gene007670.t1 MTR_8g079492 38.702 447 206 10 2 436 13 403 9.75e-82 278
MS.gene007670.t1 MTR_5g076270 39.186 393 186 7 5 388 17 365 1.95e-79 271
MS.gene007670.t1 MTR_2g006270 35.683 454 210 11 3 447 4 384 1.83e-77 255
MS.gene007670.t1 MTR_4g088210 40.365 384 170 9 12 381 38 376 2.66e-77 262
MS.gene007670.t1 MTR_4g088210 40.365 384 170 9 12 381 38 376 3.24e-77 262
MS.gene007670.t1 MTR_8g100050 38.847 399 185 7 12 399 59 409 4.51e-77 262
MS.gene007670.t1 MTR_8g100050 38.847 399 185 7 12 399 59 409 1.22e-76 263
MS.gene007670.t1 MTR_3g064050 37.740 416 204 8 6 412 20 389 4.50e-76 261
MS.gene007670.t1 MTR_1g024025 40.155 386 175 8 6 381 37 376 2.14e-75 265
MS.gene007670.t1 MTR_2g093740 38.517 418 191 9 12 410 56 426 1.30e-74 254
MS.gene007670.t1 MTR_2g093740 38.517 418 191 9 12 410 56 426 6.08e-74 254
MS.gene007670.t1 MTR_2g093740 38.517 418 191 9 12 410 56 426 1.04e-73 254
MS.gene007670.t1 MTR_1g058210 37.192 406 185 10 9 401 9 357 2.39e-73 244
MS.gene007670.t1 MTR_4g124900 35.600 500 241 14 3 484 14 450 9.75e-73 256
MS.gene007670.t1 MTR_4g124900 35.600 500 241 14 3 484 14 450 1.08e-72 255
MS.gene007670.t1 MTR_5g040740 37.817 394 169 8 9 391 9 337 2.59e-72 240
MS.gene007670.t1 MTR_8g027440 31.285 716 359 26 3 670 16 646 3.19e-72 248
MS.gene007670.t1 MTR_4g060460 38.200 411 186 6 12 402 54 416 1.87e-71 248
MS.gene007670.t1 MTR_2g043250 38.010 392 184 7 3 383 14 357 1.93e-71 252
MS.gene007670.t1 MTR_4g060460 38.200 411 186 6 12 402 54 416 1.98e-71 248
MS.gene007670.t1 MTR_2g093740 37.355 431 191 10 12 410 56 439 6.52e-71 244
MS.gene007670.t1 MTR_2g005240 36.989 465 203 14 12 440 23 433 1.71e-70 243
MS.gene007670.t1 MTR_2g093740 37.355 431 191 10 12 410 56 439 2.94e-70 244
MS.gene007670.t1 MTR_2g093740 37.355 431 191 10 12 410 56 439 4.26e-70 244
MS.gene007670.t1 MTR_7g062540 37.081 418 196 13 1 404 3 367 1.50e-67 235
MS.gene007670.t1 MTR_8g101360 36.735 392 195 7 2 384 21 368 2.00e-63 229
MS.gene007670.t1 MTR_2g014770 49.296 213 99 2 6 209 47 259 3.74e-58 207
MS.gene007670.t1 MTR_2g014770 37.963 108 66 1 274 381 281 387 2.75e-18 89.4
MS.gene007670.t1 MTR_2g006380 32.675 456 206 16 7 447 15 384 9.18e-58 202
MS.gene007670.t1 MTR_8g079492 37.534 373 175 10 67 436 3 320 1.18e-57 210
MS.gene007670.t1 MTR_2g014770 49.296 213 99 2 6 209 47 259 1.19e-57 207
MS.gene007670.t1 MTR_2g014770 31.183 186 114 5 274 446 281 465 1.55e-18 90.5
MS.gene007670.t1 MTR_5g061890 36.935 398 166 15 9 387 9 340 9.39e-57 198
MS.gene007670.t1 MTR_5g060780 36.224 392 168 14 9 382 9 336 3.57e-56 197
MS.gene007670.t1 MTR_5g460920 35.623 393 169 14 9 382 9 336 3.31e-55 194
MS.gene007670.t1 MTR_2g093740 40.373 322 142 7 91 410 23 296 7.13e-55 199
MS.gene007670.t1 MTR_2g093740 39.441 322 145 6 91 410 23 296 1.43e-54 199
MS.gene007670.t1 MTR_4g021580 49.528 212 99 4 7 211 14 224 2.83e-53 194
MS.gene007670.t1 MTR_4g021580 49.528 212 99 4 7 211 14 224 4.06e-53 194
MS.gene007670.t1 MTR_2g093740 38.806 335 142 8 91 410 23 309 2.41e-51 189
MS.gene007670.t1 MTR_2g093740 37.910 335 145 7 91 410 23 309 3.02e-51 189
MS.gene007670.t1 MTR_8g446900 31.608 367 191 8 56 422 12 318 3.46e-50 180
MS.gene007670.t1 MTR_5g060630 31.860 430 201 14 9 398 9 386 1.33e-48 179
MS.gene007670.t1 MTR_5g460740 31.860 430 201 14 9 398 9 386 1.33e-48 179
MS.gene007670.t1 MTR_5g062970 43.878 196 92 3 12 196 38 226 2.45e-47 167
MS.gene007670.t1 MTR_5g062960 37.234 188 90 5 275 458 1 164 3.22e-25 104
MS.gene007670.t1 MTR_3g064050 39.231 130 75 3 284 412 26 152 5.24e-17 85.5
MS.gene007670.t1 MTR_5g032260 39.048 105 62 2 275 378 49 152 9.02e-14 74.3
MS.gene007670.t1 MTR_5g060770 29.670 182 108 6 55 227 6 176 1.40e-13 73.2
MS.gene007670.t1 MTR_1g058350 32.432 148 83 4 103 238 71 213 4.80e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene007670.t1 AT2G28350 58.108 740 217 21 3 697 2 693 0.0 753
MS.gene007670.t1 AT4G30080 56.023 689 248 14 4 669 12 668 0.0 716
MS.gene007670.t1 AT2G28350 57.644 713 209 21 3 670 2 666 0.0 714
MS.gene007670.t1 AT1G77850 42.918 473 233 12 9 467 16 465 1.88e-116 362
MS.gene007670.t1 AT1G77850 42.918 473 233 12 9 467 16 465 3.83e-116 361
MS.gene007670.t1 AT1G59750 31.930 689 339 20 12 662 22 618 4.68e-82 273
MS.gene007670.t1 AT1G59750 32.122 688 341 20 12 662 22 620 6.40e-82 273
MS.gene007670.t1 AT1G59750 32.075 689 343 20 12 662 22 623 2.55e-81 272
MS.gene007670.t1 AT1G59750 32.075 689 343 20 12 662 22 623 2.55e-81 272
MS.gene007670.t1 AT1G30330 40.097 414 193 8 3 407 14 381 5.70e-81 276
MS.gene007670.t1 AT1G30330 40.049 412 192 8 5 407 18 383 2.07e-80 275
MS.gene007670.t1 AT1G30330 40.049 412 192 8 5 407 18 383 2.07e-80 275
MS.gene007670.t1 AT5G37020 38.817 389 185 7 9 388 19 363 3.79e-79 268
MS.gene007670.t1 AT5G37020 38.817 389 185 7 9 388 19 363 9.02e-79 268
MS.gene007670.t1 AT2G33860 33.733 501 244 13 12 474 54 504 2.12e-76 257
MS.gene007670.t1 AT5G60450 38.916 406 183 9 12 400 41 398 5.21e-76 259
MS.gene007670.t1 AT5G60450 38.916 406 183 9 12 400 65 422 9.12e-76 259
MS.gene007670.t1 AT5G62000 39.409 406 185 10 9 400 52 410 4.22e-74 256
MS.gene007670.t1 AT5G62000 39.409 406 185 10 9 400 52 410 4.22e-74 256
MS.gene007670.t1 AT5G62000 39.409 406 185 10 9 400 52 410 4.22e-74 256
MS.gene007670.t1 AT5G62000 39.409 406 185 10 9 400 52 410 4.22e-74 256
MS.gene007670.t1 AT5G62000 39.409 406 185 10 9 400 52 410 6.60e-74 256
MS.gene007670.t1 AT2G46530 39.005 382 169 12 12 381 21 350 3.06e-67 232
MS.gene007670.t1 AT2G46530 39.005 382 169 12 12 381 42 371 5.51e-67 232
MS.gene007670.t1 AT1G19850 37.500 392 182 9 4 381 35 377 8.69e-66 234
MS.gene007670.t1 AT1G19850 37.500 392 182 9 4 381 46 388 8.78e-66 234
MS.gene007670.t1 AT3G61830 36.131 429 206 13 4 418 14 388 1.63e-65 228
MS.gene007670.t1 AT1G35520 29.970 674 348 24 6 660 21 589 3.54e-61 216
MS.gene007670.t1 AT1G34390 28.299 682 361 22 6 667 21 594 1.26e-59 211
MS.gene007670.t1 AT1G35240 36.616 396 182 14 6 387 21 361 4.01e-58 207
MS.gene007670.t1 AT1G34310 35.539 408 196 13 6 402 21 372 6.81e-58 206
MS.gene007670.t1 AT4G23980 35.025 394 177 12 8 381 8 342 2.06e-57 206
MS.gene007670.t1 AT1G19220 48.869 221 96 4 3 211 14 229 2.08e-57 211
MS.gene007670.t1 AT1G19220 40.000 130 67 4 286 412 261 382 2.48e-17 87.0
MS.gene007670.t1 AT4G23980 35.025 394 177 12 8 381 8 342 2.22e-57 206
MS.gene007670.t1 AT1G35540 34.913 401 187 13 6 395 21 358 5.49e-57 204
MS.gene007670.t1 AT5G37020 38.699 292 134 5 98 388 21 268 6.99e-57 206
MS.gene007670.t1 AT1G34410 35.659 387 183 12 6 381 21 352 7.69e-57 204
MS.gene007670.t1 AT5G20730 47.059 221 100 4 3 211 15 230 6.46e-56 207
MS.gene007670.t1 AT5G20730 42.478 113 64 1 286 398 262 373 3.20e-18 90.1
MS.gene007670.t1 AT5G20730 47.059 221 100 4 3 211 15 230 6.67e-56 207
MS.gene007670.t1 AT5G20730 42.105 114 64 2 286 398 262 374 5.66e-17 86.3
MS.gene007670.t1 AT5G20730 47.059 221 100 4 3 211 15 230 7.10e-56 207
MS.gene007670.t1 AT5G20730 42.105 114 64 2 286 398 262 374 5.05e-17 86.3
MS.gene007670.t1 AT2G33860 32.576 396 188 10 108 474 2 347 2.99e-54 193
MS.gene007670.t1 AT1G34170 33.333 387 194 12 6 381 19 352 1.19e-52 190
MS.gene007670.t1 AT3G61830 35.946 370 180 10 52 418 22 337 1.38e-52 191
MS.gene007670.t1 AT2G46530 38.406 276 121 6 107 381 36 263 3.17e-46 172
MS.gene007670.t1 AT1G34170 39.091 220 117 5 6 214 19 232 2.71e-42 160
MS.gene007670.t1 AT1G34170 39.091 220 117 5 6 214 19 232 8.74e-42 160
MS.gene007670.t1 AT1G43950 39.810 211 109 6 6 207 21 222 2.42e-38 142
MS.gene007670.t1 AT2G36080 37.838 111 63 3 103 208 33 142 1.78e-11 63.9
MS.gene007670.t1 AT2G36080 37.838 111 63 3 103 208 33 142 1.78e-11 63.9
MS.gene007670.t1 AT2G36080 37.838 111 63 3 103 208 33 142 4.14e-11 64.3
MS.gene007670.t1 AT2G36080 37.838 111 63 3 103 208 33 142 4.67e-11 63.9

Find 160 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTAGGATTAGTTGGTTTA+TGG 0.143329 4.3:-42446157 MS.gene007670:CDS
TTGAAACTGAAGACTCTTCT+AGG 0.222931 4.3:-42446173 MS.gene007670:CDS
ACCCTTGATTTATCACTTCT+TGG 0.257467 4.3:-42444739 MS.gene007670:CDS
GGAAAGTGAGAAAGGGTATT+TGG 0.265159 4.3:-42447141 MS.gene007670:CDS
TTGACAATTCTGACAGATTC+AGG 0.268164 4.3:-42444233 MS.gene007670:CDS
AAAACTTTGGAGTTCACTTT+AGG 0.275701 4.3:+42447074 None:intergenic
TTCTCCGTGGCGCCTTCTTC+AGG 0.276881 4.3:-42446088 MS.gene007670:intron
TCATCTTGTGGATGGACTTC+AGG 0.278431 4.3:-42446489 MS.gene007670:CDS
CTAATACAAACATTGATCTA+AGG 0.281084 4.3:-42447022 MS.gene007670:CDS
AACAATGTAAATTGTGGAAT+TGG 0.287100 4.3:-42446456 MS.gene007670:CDS
CAGGCATGATAAGCTTTCTT+TGG 0.290621 4.3:+42445050 None:intergenic
TGGAATTCGTAGGGCGAAAA+AGG 0.294025 4.3:-42446535 MS.gene007670:CDS
GGGCCAAACAGCCCCTTCTT+TGG 0.299415 4.3:-42445261 MS.gene007670:CDS
GGAGCCAGGCATGCTCAATT+TGG 0.301173 4.3:-42445207 MS.gene007670:CDS
TGTGGATGGACTTCAGGTTC+TGG 0.309717 4.3:-42446483 MS.gene007670:CDS
AGAAGGCGCCACGGAGAATT+AGG 0.313389 4.3:+42446093 None:intergenic
GGGAACAATTGCTTCTGTTC+AGG 0.316761 4.3:-42446136 MS.gene007670:CDS
CACCAGCAACAAGCTTCTTC+TGG 0.317024 4.3:+42446601 None:intergenic
ATGAGGATTCAATGGTGTTC+TGG 0.321716 4.3:-42446216 MS.gene007670:CDS
TCTAGGATTAGTTGGTTTAT+GGG 0.340586 4.3:-42446156 MS.gene007670:CDS
CGCCGGCACTTGCTTACAAC+AGG 0.344728 4.3:-42446642 MS.gene007670:CDS
AATTGTGGAATTGGACCTTT+TGG 0.346313 4.3:-42446447 MS.gene007670:CDS
TGGCAACTTGGATTGGATAT+TGG 0.355146 4.3:-42444802 MS.gene007670:CDS
TAATGAAGCTTCTTCCTTTG+AGG 0.360496 4.3:-42446917 MS.gene007670:CDS
AGCAACAATAAACTTCAGTT+AGG 0.363663 4.3:-42445156 MS.gene007670:CDS
TTCACCACTGATTCTTTGTA+AGG 0.370999 4.3:-42446991 MS.gene007670:CDS
GGTTGTTGGTGTTAAATTCA+TGG 0.380615 4.3:-42446970 MS.gene007670:CDS
TGTTATGGAAGCTGTGAAAT+TGG 0.385027 4.3:-42446328 MS.gene007670:CDS
AGTGAGGGCTGAAGATGTTA+TGG 0.385830 4.3:-42446343 MS.gene007670:CDS
AAACCAAAGAAGGGGCTGTT+TGG 0.394320 4.3:+42445258 None:intergenic
CAATGCTCCTGCATGTATAC+AGG 0.395010 4.3:-42445229 MS.gene007670:CDS
AGGATGTGCACGGTGAGCTT+TGG 0.396378 4.3:-42446695 MS.gene007670:CDS
AAGACTCTTCTAGGATTAGT+TGG 0.399400 4.3:-42446164 MS.gene007670:CDS
GAGAATAAGACATTGAGAAA+TGG 0.411485 4.3:-42446399 MS.gene007670:CDS
TTCTTCCTCGGTGGAGTGAA+AGG 0.420088 4.3:+42445360 None:intergenic
AGGTGGTGGTGGTGATGGTT+TGG 0.423416 4.3:-42446871 MS.gene007670:CDS
TTCGCCACCACCGTCTGAAC+AGG 0.425252 4.3:+42446717 None:intergenic
CTGATTCTTTGTAAGGTTGT+TGG 0.426252 4.3:-42446984 MS.gene007670:CDS
CTAATGCTGCTGAGTTTAGT+TGG 0.428567 4.3:-42444822 MS.gene007670:CDS
GCCTACTACTACAGGTTACA+TGG 0.430758 4.3:-42445470 MS.gene007670:intron
GGATAGTAAACAACTTCAAA+TGG 0.432142 4.3:+42446291 None:intergenic
ACGGTGAGCTTTGGAAGTTT+AGG 0.437969 4.3:-42446686 MS.gene007670:CDS
TGAGGATTCAATGGTGTTCT+GGG 0.446254 4.3:-42446215 MS.gene007670:CDS
AACCAAGAAGTGATAAATCA+AGG 0.446549 4.3:+42444737 None:intergenic
TTCAATGGTGTTCTGGGATG+AGG 0.448575 4.3:-42446209 MS.gene007670:CDS
TCCTCCAAATTGAGCATGCC+TGG 0.451469 4.3:+42445203 None:intergenic
ACTGAAGTTTATTGTTGCTA+AGG 0.452992 4.3:+42445160 None:intergenic
AACTCGGGAGTGCTTGCTCT+TGG 0.454030 4.3:+42446270 None:intergenic
GATGAAGGTGGTGGTGGTGA+TGG 0.454860 4.3:-42446876 MS.gene007670:CDS
CATTATGCAAATATGCTTAG+AGG 0.456170 4.3:+42444029 None:intergenic
CAGCTATGGCATGCTTGTGC+TGG 0.456859 4.3:-42447113 MS.gene007670:CDS
TATCATGCCTGTTGAGTATC+GGG 0.463632 4.3:-42445038 MS.gene007670:CDS
AGCACCTTGATGTGCATAGT+AGG 0.468204 4.3:-42445113 MS.gene007670:CDS
TCAAGGTGCTGAATGTTAGT+AGG 0.470920 4.3:+42445126 None:intergenic
AAGTTTAGGCATATTTATAG+AGG 0.471597 4.3:-42446672 MS.gene007670:CDS
AACCAGAAGAAGCTTGTTGC+TGG 0.473424 4.3:-42446603 MS.gene007670:CDS
GCTGAGTTTAGTTGGCAACT+TGG 0.473727 4.3:-42444814 MS.gene007670:CDS
TCAGAGCTTGGTTATGATGA+AGG 0.480927 4.3:-42446891 MS.gene007670:CDS
TTGGATGCATCAAACAAAAC+AGG 0.481900 4.3:-42444053 MS.gene007670:CDS
TCAATGAAGGAAAGTGAGAA+AGG 0.482138 4.3:-42447149 MS.gene007670:CDS
ATTGTCAGATAAACCAAAGA+AGG 0.484820 4.3:+42445248 None:intergenic
GGTTATGATGAAGGTGGTGG+TGG 0.486406 4.3:-42446882 MS.gene007670:CDS
TGAGTGGAAGAGTGAGAGTG+AGG 0.488015 4.3:-42446359 MS.gene007670:CDS
TCACAAAGGTGAACATGGAT+TGG 0.488178 4.3:-42444072 MS.gene007670:CDS
GTTTAGTTGGCAACTTGGAT+TGG 0.492268 4.3:-42444809 MS.gene007670:CDS
GCAACAATAAACTTCAGTTA+GGG 0.494974 4.3:-42445155 MS.gene007670:CDS
TGTTAAGGCTTCAGCTGTGA+AGG 0.496289 4.3:-42446244 MS.gene007670:CDS
AGGGGCTGTTTGGCCCGAGC+TGG 0.496697 4.3:+42445268 None:intergenic
GGTTCTAACAATGTAAATTG+TGG 0.497055 4.3:-42446462 MS.gene007670:CDS
TTATCATGCCTGTTGAGTAT+CGG 0.500678 4.3:-42445039 MS.gene007670:CDS
TGTTCAGACGGTGGTGGCGA+AGG 0.501569 4.3:-42446715 MS.gene007670:CDS
AGAAGAGTCTTCAGTTTCAA+AGG 0.502379 4.3:+42446176 None:intergenic
TTGATGGTTCATCTTGTGGA+TGG 0.504684 4.3:-42446497 MS.gene007670:CDS
AATCTGATGCAAACAATGGT+GGG 0.510020 4.3:-42446806 MS.gene007670:CDS
CTATGGCATGCTTGTGCTGG+TGG 0.512929 4.3:-42447110 MS.gene007670:CDS
GCATGCTTGTGCTGGTGGAA+TGG 0.513876 4.3:-42447105 MS.gene007670:CDS
AGCTGAGCCGCCTGTTCAGA+CGG 0.518924 4.3:-42446727 MS.gene007670:CDS
AGTAAACAACTTCAAATGGT+TGG 0.519885 4.3:+42446295 None:intergenic
TTGTGGAAAGTAGAAAACTT+TGG 0.520773 4.3:+42447061 None:intergenic
CCTTTGAGGAATTCAGAGCT+TGG 0.522861 4.3:-42446903 MS.gene007670:CDS
AAATCGGTGAAGAACCCTTC+AGG 0.523462 4.3:-42444612 MS.gene007670:intron
CTGCATGTATACAGGGAGCC+AGG 0.523472 4.3:-42445221 MS.gene007670:CDS
GAAGAGTCTTCAGTTTCAAA+GGG 0.524779 4.3:+42446177 None:intergenic
GGAAACCGCAGCTTCTTCCT+CGG 0.526125 4.3:+42445348 None:intergenic
TAATACAAACATTGATCTAA+GGG 0.528805 4.3:-42447021 MS.gene007670:CDS
TGAAGCCTTAACACAAAACT+CGG 0.529140 4.3:+42446254 None:intergenic
ATTTGTTGCTCAGTGAGTAT+TGG 0.534131 4.3:+42444934 None:intergenic
TCTTCCTCGGTGGAGTGAAA+GGG 0.536181 4.3:+42445361 None:intergenic
CAATGAAGGAAAGTGAGAAA+GGG 0.542351 4.3:-42447148 MS.gene007670:CDS
ACCCTGTTGTAAGCAAGTGC+CGG 0.547262 4.3:+42446640 None:intergenic
GCATGTTCAGCATGTCCTTG+TGG 0.548555 4.3:+42447044 None:intergenic
CAGCTGTGAAGGCTGCTATG+AGG 0.549739 4.3:-42446233 MS.gene007670:CDS
CTGGCTCCCTGTATACATGC+AGG 0.552767 4.3:+42445222 None:intergenic
GATACTAATTCGACCAACCA+CGG 0.553761 4.3:+42445408 None:intergenic
AATTGTGTGTTGGAATTCGT+AGG 0.556594 4.3:-42446545 MS.gene007670:CDS
ATCGTCTCAGCACAGTACCT+TGG 0.557673 4.3:+42446771 None:intergenic
TCTGATGCAAACAATGGTGG+GGG 0.561128 4.3:-42446804 MS.gene007670:CDS
GCCGCCTGTTCAGACGGTGG+TGG 0.561486 4.3:-42446721 MS.gene007670:CDS
ATACTAATTCGACCAACCAC+GGG 0.564828 4.3:+42445409 None:intergenic
GCCAGGCATGCTCAATTTGG+AGG 0.565841 4.3:-42445204 MS.gene007670:CDS
ATTGGATATTGGTCATTGCA+AGG 0.566046 4.3:-42444791 MS.gene007670:CDS
AGGTTGTTGATCCTATCCGC+TGG 0.566285 4.3:-42446116 MS.gene007670:CDS
GGAACTCACAAAGGTGAACA+TGG 0.567844 4.3:-42444077 MS.gene007670:CDS
GAAGCGTGTTAGCCCGTGGT+TGG 0.567936 4.3:-42445421 MS.gene007670:CDS
TTGTCAGATAAACCAAAGAA+GGG 0.569032 4.3:+42445249 None:intergenic
ATAGTAAAGACAAAGAGAAG+TGG 0.569647 4.3:-42444876 MS.gene007670:CDS
CAATCTGATGCAAACAATGG+TGG 0.570087 4.3:-42446807 MS.gene007670:CDS
AGTTTAGGCATATTTATAGA+GGG 0.570327 4.3:-42446671 MS.gene007670:CDS
GCTTGTTGCTGGTGATTCTG+TGG 0.572232 4.3:-42446592 MS.gene007670:CDS
ACCAAGAAGTGATAAATCAA+GGG 0.572563 4.3:+42444738 None:intergenic
ATCTGATGCAAACAATGGTG+GGG 0.580572 4.3:-42446805 MS.gene007670:CDS
AAAGTCTTAGCAAAAGATGC+AGG 0.580849 4.3:+42446834 None:intergenic
CCAAGCTCTGAATTCCTCAA+AGG 0.581047 4.3:+42446903 None:intergenic
TCAGCTGCGTAATCAAGCCT+TGG 0.583950 4.3:+42446744 None:intergenic
ACAGGTGCTGTGAAGCAAAT+CGG 0.584233 4.3:-42444628 MS.gene007670:CDS
AAATCCTACTATGCACATCA+AGG 0.584801 4.3:+42445109 None:intergenic
GAGAATGGTGAATTGTGTGT+TGG 0.591957 4.3:-42446555 MS.gene007670:CDS
GTGCTGAGACGATCTTTCCA+AGG 0.594168 4.3:-42446761 MS.gene007670:CDS
GAGTGGAAGAGTGAGAGTGA+GGG 0.596583 4.3:-42446358 MS.gene007670:CDS
TTTATAGAGGGACACCGCGC+CGG 0.597722 4.3:-42446659 MS.gene007670:CDS
TTGCCTGAAAACGTCGGTGA+CGG 0.603464 4.3:+42445291 None:intergenic
TGGAGTCAGAAGATGTTGGA+AGG 0.603517 4.3:-42444762 MS.gene007670:CDS
AGGCTGCTATGAGGATTCAA+TGG 0.603915 4.3:-42446224 MS.gene007670:CDS
CTCCACCGAGGAAGAAGCTG+CGG 0.604549 4.3:-42445353 MS.gene007670:CDS
GGTATTTGGATCCACAGCTA+TGG 0.606132 4.3:-42447127 MS.gene007670:CDS
AACCGCAGCTTCTTCCTCGG+TGG 0.613433 4.3:+42445351 None:intergenic
GGAGAATTAGGCCAGCGGAT+AGG 0.613434 4.3:+42446105 None:intergenic
GCCACCACCGTCTGAACAGG+CGG 0.614156 4.3:+42446720 None:intergenic
TTCTTGGAGTCAGAAGATGT+TGG 0.615581 4.3:-42444766 MS.gene007670:CDS
AAAGGGCTGAAATGAATGAC+TGG 0.619094 4.3:+42445378 None:intergenic
CCTACTACTACAGGTTACAT+GGG 0.623681 4.3:-42445469 MS.gene007670:intron
TGACGGAAACTGAACATCGA+GGG 0.624449 4.3:+42445308 None:intergenic
CTTGGTTATGATGAAGGTGG+TGG 0.625908 4.3:-42446885 MS.gene007670:CDS
GCACAAGCATGCCATAGCTG+TGG 0.631502 4.3:+42447116 None:intergenic
TGAGCCGCCTGTTCAGACGG+TGG 0.636259 4.3:-42446724 MS.gene007670:CDS
ACACAAAAGCAATACCTGAA+GGG 0.638283 4.3:+42444598 None:intergenic
ATTGTGTGTTGGAATTCGTA+GGG 0.641411 4.3:-42446544 MS.gene007670:CDS
GACGGAAACTGAACATCGAG+GGG 0.641637 4.3:+42445309 None:intergenic
AATGCTCCTGCATGTATACA+GGG 0.643769 4.3:-42445228 MS.gene007670:CDS
GGCTGACCCTGAAACTGATG+AGG 0.645568 4.3:-42446949 MS.gene007670:CDS
ACGTGAAGCGTGTTAGCCCG+TGG 0.646883 4.3:-42445425 MS.gene007670:CDS
AATACCTGAAGAAGGCGCCA+CGG 0.652216 4.3:+42446084 None:intergenic
TCACAGCACCTGTTGCATCA+CGG 0.662294 4.3:+42444638 None:intergenic
GAAGCCTTAACACAAAACTC+GGG 0.664478 4.3:+42446255 None:intergenic
GTTGTAAGCAAGTGCCGGCG+CGG 0.664793 4.3:+42446645 None:intergenic
TCCGCTGGCCTAATTCTCCG+TGG 0.672854 4.3:-42446101 MS.gene007670:CDS
GGCACTTGCTTACAACAGGG+TGG 0.673448 4.3:-42446638 MS.gene007670:CDS
TGGATAACAGGAACTCACAA+AGG 0.673838 4.3:-42444086 MS.gene007670:CDS
TGTCAGATAAACCAAAGAAG+GGG 0.674937 4.3:+42445250 None:intergenic
AGCTGGTTGCCTGAAAACGT+CGG 0.679058 4.3:+42445285 None:intergenic
ACAACCTTACAAAGAATCAG+TGG 0.680398 4.3:+42446987 None:intergenic
CTGATGCAAACAATGGTGGG+GGG 0.689649 4.3:-42446803 MS.gene007670:CDS
GCCGGCACTTGCTTACAACA+GGG 0.691948 4.3:-42446641 MS.gene007670:CDS
GCCACGGAGAATTAGGCCAG+CGG 0.695316 4.3:+42446100 None:intergenic
GAGCTTGGTTATGATGAAGG+TGG 0.703479 4.3:-42446888 MS.gene007670:CDS
ACTCAATCTGATGCAAACAA+TGG 0.706199 4.3:-42446810 MS.gene007670:CDS
GTGACGGAAACTGAACATCG+AGG 0.721853 4.3:+42445307 None:intergenic
GTGGTGGCGAAGGATGTGCA+CGG 0.739739 4.3:-42446705 MS.gene007670:CDS
ACGGAAACTGAACATCGAGG+GGG 0.755057 4.3:+42445310 None:intergenic
TCAGCCCTTTCACTCCACCG+AGG 0.764350 4.3:-42445365 MS.gene007670:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGAAACAATTAGTAGTAT+AGG + chr4.3:42445322-42445341 None:intergenic 20.0%
!! GAAATGTCTTATATAATAAG+AGG - chr4.3:42447046-42447065 MS.gene007670:CDS 20.0%
!! TAATACAAACATTGATCTAA+GGG - chr4.3:42444156-42444175 MS.gene007670:intron 20.0%
!!! AAAATAGAGTAAAGTCATTT+TGG + chr4.3:42446814-42446833 None:intergenic 20.0%
!!! ATTGTTTTTCAGTTACAATT+TGG - chr4.3:42445113-42445132 MS.gene007670:CDS 20.0%
!!! TTGAACACATTTTTACATAA+GGG - chr4.3:42445146-42445165 MS.gene007670:CDS 20.0%
!!! TTTGTTATTGTTCAGTTTTA+TGG - chr4.3:42445205-42445224 MS.gene007670:CDS 20.0%
! AAAAAAGAAAATGCTCCAAA+AGG + chr4.3:42444748-42444767 None:intergenic 25.0%
! AACAATGTAAATTGTGGAAT+TGG - chr4.3:42444721-42444740 MS.gene007670:CDS 25.0%
! AAGTTTAGGCATATTTATAG+AGG - chr4.3:42444505-42444524 MS.gene007670:intron 25.0%
! AGTATTACTCTTGGATTAAT+GGG - chr4.3:42446874-42446893 MS.gene007670:CDS 25.0%
! AGTTTAGGCATATTTATAGA+GGG - chr4.3:42444506-42444525 MS.gene007670:intron 25.0%
! CTAATACAAACATTGATCTA+AGG - chr4.3:42444155-42444174 MS.gene007670:intron 25.0%
! TAAGTTTCACTATACATTCT+AGG - chr4.3:42446756-42446775 MS.gene007670:CDS 25.0%
! TAGCAAAATAGTATTACTCT+TGG - chr4.3:42446865-42446884 MS.gene007670:CDS 25.0%
! TAGTATTACTCTTGGATTAA+TGG - chr4.3:42446873-42446892 MS.gene007670:CDS 25.0%
!! ATTTTGCTACTGTACAATAA+TGG + chr4.3:42446854-42446873 None:intergenic 25.0%
!! GTTGAACACATTTTTACATA+AGG - chr4.3:42445145-42445164 MS.gene007670:CDS 25.0%
!! TTTTGCTACTGTACAATAAT+GGG + chr4.3:42446853-42446872 None:intergenic 25.0%
!!! AAACATCAGTTTTGTCTTTT+TGG - chr4.3:42446216-42446235 MS.gene007670:CDS 25.0%
AACCAAGAAGTGATAAATCA+AGG + chr4.3:42446443-42446462 None:intergenic 30.0%
ACCAAGAAGTGATAAATCAA+GGG + chr4.3:42446442-42446461 None:intergenic 30.0%
ACTGAAGTTTATTGTTGCTA+AGG + chr4.3:42446020-42446039 None:intergenic 30.0%
AGCAACAATAAACTTCAGTT+AGG - chr4.3:42446021-42446040 MS.gene007670:intron 30.0%
AGTAAACAACTTCAAATGGT+TGG + chr4.3:42444885-42444904 None:intergenic 30.0%
ATAGTAAAGACAAAGAGAAG+TGG - chr4.3:42446301-42446320 MS.gene007670:CDS 30.0%
ATTGTCAGATAAACCAAAGA+AGG + chr4.3:42445932-42445951 None:intergenic 30.0%
GAAACAATTAGTAGTATAGG+AGG + chr4.3:42445319-42445338 None:intergenic 30.0%
GAAATAACCTGTGTTAAGTA+GGG - chr4.3:42445580-42445599 MS.gene007670:intron 30.0%
GAGAATAAGACATTGAGAAA+TGG - chr4.3:42444778-42444797 MS.gene007670:CDS 30.0%
GCAACAATAAACTTCAGTTA+GGG - chr4.3:42446022-42446041 MS.gene007670:intron 30.0%
GGATAGTAAACAACTTCAAA+TGG + chr4.3:42444889-42444908 None:intergenic 30.0%
GTATTACTCTTGGATTAATG+GGG - chr4.3:42446875-42446894 MS.gene007670:CDS 30.0%
TATTACTCTTGGATTAATGG+GGG - chr4.3:42446876-42446895 MS.gene007670:CDS 30.0%
TGAAAAACAATACCTGAAGA+AGG + chr4.3:42445104-42445123 None:intergenic 30.0%
TGAAATAACCTGTGTTAAGT+AGG - chr4.3:42445579-42445598 MS.gene007670:intron 30.0%
TTGTAGACTAAACTAACAAC+CGG + chr4.3:42446720-42446739 None:intergenic 30.0%
TTGTCAGATAAACCAAAGAA+GGG + chr4.3:42445931-42445950 None:intergenic 30.0%
TTGTGGAAAGTAGAAAACTT+TGG + chr4.3:42444119-42444138 None:intergenic 30.0%
! AAAGTTTTCTACTTTCCACA+AGG - chr4.3:42444118-42444137 MS.gene007670:intron 30.0%
! GACTCAATTTTATGCTTCTT+TGG - chr4.3:42445168-42445187 MS.gene007670:CDS 30.0%
! GGTTCTAACAATGTAAATTG+TGG - chr4.3:42444715-42444734 MS.gene007670:CDS 30.0%
! TTCACGTTTTGTAGTAAATC+AGG + chr4.3:42445739-42445758 None:intergenic 30.0%
! TTTTCACTCTTGTGATTATG+AGG - chr4.3:42445555-42445574 MS.gene007670:intron 30.0%
!! AAAACTTTGGAGTTCACTTT+AGG + chr4.3:42444106-42444125 None:intergenic 30.0%
!! AGGATCTTGATTGTTTTTCA+GGG - chr4.3:42447066-42447085 MS.gene007670:CDS 30.0%
!! TCTAGGATTAGTTGGTTTAT+GGG - chr4.3:42445021-42445040 MS.gene007670:CDS 30.0%
!! TTCTAGGATTAGTTGGTTTA+TGG - chr4.3:42445020-42445039 MS.gene007670:CDS 30.0%
!!! GTGAACTTTTTTCAAACTCT+TGG - chr4.3:42445527-42445546 MS.gene007670:intron 30.0%
!!! GTGTCTTTTGCTAGTTTTTT+TGG - chr4.3:42446693-42446712 MS.gene007670:CDS 30.0%
!!! TGTTTTGATGGTAATTTGAG+TGG - chr4.3:42444802-42444821 MS.gene007670:CDS 30.0%
!!! TTGAGAAATGGTTGTTTTGA+TGG - chr4.3:42444790-42444809 MS.gene007670:CDS 30.0%
!!! TTTTGCTAGTTTTTTTGGTC+CGG - chr4.3:42446698-42446717 MS.gene007670:CDS 30.0%
AAAGTCTTAGCAAAAGATGC+AGG + chr4.3:42444346-42444365 None:intergenic 35.0%
AAATCCTACTATGCACATCA+AGG + chr4.3:42446071-42446090 None:intergenic 35.0%
AACACAAAAGCAATACCTGA+AGG + chr4.3:42446583-42446602 None:intergenic 35.0%
AATCTGATGCAAACAATGGT+GGG - chr4.3:42444371-42444390 MS.gene007670:intron 35.0%
AATTGTGTGTTGGAATTCGT+AGG - chr4.3:42444632-42444651 MS.gene007670:CDS 35.0%
ACAACCTTACAAAGAATCAG+TGG + chr4.3:42444193-42444212 None:intergenic 35.0%
ACACAAAAGCAATACCTGAA+GGG + chr4.3:42446582-42446601 None:intergenic 35.0%
ACCCTTGATTTATCACTTCT+TGG - chr4.3:42446438-42446457 MS.gene007670:CDS 35.0%
ACTCAATCTGATGCAAACAA+TGG - chr4.3:42444367-42444386 MS.gene007670:intron 35.0%
AGAAGAGTCTTCAGTTTCAA+AGG + chr4.3:42445004-42445023 None:intergenic 35.0%
ATTACTCTTGGATTAATGGG+GGG - chr4.3:42446877-42446896 MS.gene007670:CDS 35.0%
ATTGGATATTGGTCATTGCA+AGG - chr4.3:42446386-42446405 MS.gene007670:CDS 35.0%
ATTGTGTGTTGGAATTCGTA+GGG - chr4.3:42444633-42444652 MS.gene007670:CDS 35.0%
ATTGTTATGCCTACTACTAC+AGG - chr4.3:42445699-42445718 MS.gene007670:intron 35.0%
CAAAAACCTCATCAGTTTCA+GGG + chr4.3:42444237-42444256 None:intergenic 35.0%
CAATCTGGGCAATAATATCA+AGG + chr4.3:42445503-42445522 None:intergenic 35.0%
CTGATTCTTTGTAAGGTTGT+TGG - chr4.3:42444193-42444212 MS.gene007670:intron 35.0%
GAAGAGTCTTCAGTTTCAAA+GGG + chr4.3:42445003-42445022 None:intergenic 35.0%
GTGATATTGTGGATAATCAC+AGG - chr4.3:42445430-42445449 MS.gene007670:CDS 35.0%
TAATGAAGCTTCTTCCTTTG+AGG - chr4.3:42444260-42444279 MS.gene007670:CDS 35.0%
TGAAGCCTTAACACAAAACT+CGG + chr4.3:42444926-42444945 None:intergenic 35.0%
TGATATTATTGCCCAGATTG+CGG - chr4.3:42445503-42445522 MS.gene007670:intron 35.0%
TGTCAGATAAACCAAAGAAG+GGG + chr4.3:42445930-42445949 None:intergenic 35.0%
TGTTATGGAAGCTGTGAAAT+TGG - chr4.3:42444849-42444868 MS.gene007670:CDS 35.0%
TTATCATGCCTGTTGAGTAT+CGG - chr4.3:42446138-42446157 MS.gene007670:CDS 35.0%
TTGAAACTGAAGACTCTTCT+AGG - chr4.3:42445004-42445023 MS.gene007670:CDS 35.0%
TTGACAATTCTGACAGATTC+AGG - chr4.3:42446944-42446963 MS.gene007670:CDS 35.0%
TTGGATGCATCAAACAAAAC+AGG - chr4.3:42447124-42447143 MS.gene007670:CDS 35.0%
! AATTGTGGAATTGGACCTTT+TGG - chr4.3:42444730-42444749 MS.gene007670:CDS 35.0%
! AGACAAAGAGAAGTGGTTTT+TGG - chr4.3:42446308-42446327 MS.gene007670:CDS 35.0%
! ATTTGTTGCTCAGTGAGTAT+TGG + chr4.3:42446246-42446265 None:intergenic 35.0%
! GTCGATTTAGCTGAAATCAT+AGG - chr4.3:42445276-42445295 MS.gene007670:CDS 35.0%
! TTTTGGATGCATTTTCAGCT+GGG - chr4.3:42446325-42446344 MS.gene007670:CDS 35.0%
! TTTTTGGATGCATTTTCAGC+TGG - chr4.3:42446324-42446343 MS.gene007670:CDS 35.0%
!! AAGACTCTTCTAGGATTAGT+TGG - chr4.3:42445013-42445032 MS.gene007670:CDS 35.0%
!! GAGGATCTTGATTGTTTTTC+AGG - chr4.3:42447065-42447084 MS.gene007670:CDS 35.0%
!! GGTTGTTGGTGTTAAATTCA+TGG - chr4.3:42444207-42444226 MS.gene007670:intron 35.0%
!! TGATTCTGTGGTGTTTTTGA+GGG - chr4.3:42444597-42444616 MS.gene007670:intron 35.0%
!! TTCACCACTGATTCTTTGTA+AGG - chr4.3:42444186-42444205 MS.gene007670:intron 35.0%
!! TTCAGTTTTATGGTCATCTC+TGG - chr4.3:42445215-42445234 MS.gene007670:CDS 35.0%
AAAGGGCTGAAATGAATGAC+TGG + chr4.3:42445802-42445821 None:intergenic 40.0%
AACACCGTCTGACATATTCA+AGG + chr4.3:42446628-42446647 None:intergenic 40.0%
AACATCTCCCTACTTAACAC+AGG + chr4.3:42445590-42445609 None:intergenic 40.0%
AATGCTCCTGCATGTATACA+GGG - chr4.3:42445949-42445968 MS.gene007670:intron 40.0%
ATACTAATTCGACCAACCAC+GGG + chr4.3:42445771-42445790 None:intergenic 40.0%
ATATTATTGCCCAGATTGCG+GGG - chr4.3:42445505-42445524 MS.gene007670:intron 40.0%
ATCTGATGCAAACAATGGTG+GGG - chr4.3:42444372-42444391 MS.gene007670:intron 40.0%
ATGAGGATTCAATGGTGTTC+TGG - chr4.3:42444961-42444980 MS.gene007670:CDS 40.0%
CAATCTGATGCAAACAATGG+TGG - chr4.3:42444370-42444389 MS.gene007670:intron 40.0%
CAGGCATGATAAGCTTTCTT+TGG + chr4.3:42446130-42446149 None:intergenic 40.0%
CATTGCTAGTCGTGATATTG+TGG - chr4.3:42445419-42445438 MS.gene007670:CDS 40.0%
CCTACTACTACAGGTTACAT+GGG - chr4.3:42445708-42445727 MS.gene007670:intron 40.0%
CTAATGCTGCTGAGTTTAGT+TGG - chr4.3:42446355-42446374 MS.gene007670:CDS 40.0%
CTCTGGTGTTTGTGGTTATA+TGG - chr4.3:42445232-42445251 MS.gene007670:CDS 40.0%
GAAGCCTTAACACAAAACTC+GGG + chr4.3:42444925-42444944 None:intergenic 40.0%
GAGAATGGTGAATTGTGTGT+TGG - chr4.3:42444622-42444641 MS.gene007670:CDS 40.0%
GATACTAATTCGACCAACCA+CGG + chr4.3:42445772-42445791 None:intergenic 40.0%
GATATTATTGCCCAGATTGC+GGG - chr4.3:42445504-42445523 MS.gene007670:intron 40.0%
GCAAAAACCTCATCAGTTTC+AGG + chr4.3:42444238-42444257 None:intergenic 40.0%
GTTTAGTTGGCAACTTGGAT+TGG - chr4.3:42446368-42446387 MS.gene007670:CDS 40.0%
TATCATGCCTGTTGAGTATC+GGG - chr4.3:42446139-42446158 MS.gene007670:CDS 40.0%
TCACAAAGGTGAACATGGAT+TGG - chr4.3:42447105-42447124 MS.gene007670:CDS 40.0%
TCAGAGCTTGGTTATGATGA+AGG - chr4.3:42444286-42444305 MS.gene007670:intron 40.0%
TCTGACATATTCAAGGACCA+AGG + chr4.3:42446621-42446640 None:intergenic 40.0%
TGAGGATTCAATGGTGTTCT+GGG - chr4.3:42444962-42444981 MS.gene007670:CDS 40.0%
TGGATAACAGGAACTCACAA+AGG - chr4.3:42447091-42447110 MS.gene007670:CDS 40.0%
TGGATAATCACAGGCAAATG+TGG - chr4.3:42445439-42445458 MS.gene007670:CDS 40.0%
TGGTCCTTGAATATGTCAGA+CGG - chr4.3:42446621-42446640 MS.gene007670:CDS 40.0%
TTCTTGGAGTCAGAAGATGT+TGG - chr4.3:42446411-42446430 MS.gene007670:CDS 40.0%
! ATCTTTTCCCGATACTCAAC+AGG + chr4.3:42446149-42446168 None:intergenic 40.0%
! TGGCAACTTGGATTGGATAT+TGG - chr4.3:42446375-42446394 MS.gene007670:CDS 40.0%
! TGGTCATTGCAAGGTTTTCT+TGG - chr4.3:42446395-42446414 MS.gene007670:CDS 40.0%
! TTGATGGTTCATCTTGTGGA+TGG - chr4.3:42444680-42444699 MS.gene007670:CDS 40.0%
!! GGAAAGTGAGAAAGGGTATT+TGG - chr4.3:42444036-42444055 MS.gene007670:CDS 40.0%
!! GGTTTTGATGGTTCATCTTG+TGG - chr4.3:42444676-42444695 MS.gene007670:CDS 40.0%
!! GTGATTCTGTGGTGTTTTTG+AGG - chr4.3:42444596-42444615 MS.gene007670:intron 40.0%
!! TATCTTTGAGATCGCAACTG+AGG + chr4.3:42445473-42445492 None:intergenic 40.0%
!! TCAAGGTGCTGAATGTTAGT+AGG + chr4.3:42446054-42446073 None:intergenic 40.0%
!! TTTTGAGTAAACAAGCGCCT+TGG - chr4.3:42446601-42446620 MS.gene007670:CDS 40.0%
!!! CTTGATTGTTTTTCAGGGCA+TGG - chr4.3:42447071-42447090 MS.gene007670:CDS 40.0%
!!! GTTTTTTACCGTGATGCAAC+AGG - chr4.3:42446531-42446550 MS.gene007670:CDS 40.0%
!!! TTTTTCAGGGCATGGATAAC+AGG - chr4.3:42447079-42447098 MS.gene007670:CDS 40.0%
AAAAAGTTCACCCCGCAATC+TGG + chr4.3:42445518-42445537 None:intergenic 45.0%
AAAAGTTCACCCCGCAATCT+GGG + chr4.3:42445517-42445536 None:intergenic 45.0%
AAACCAAAGAAGGGGCTGTT+TGG + chr4.3:42445922-42445941 None:intergenic 45.0%
AAATCGGTGAAGAACCCTTC+AGG - chr4.3:42446565-42446584 MS.gene007670:CDS 45.0%
AGCACCTTGATGTGCATAGT+AGG - chr4.3:42446064-42446083 MS.gene007670:intron 45.0%
AGCTCAATTGTCTTACTGCG+AGG - chr4.3:42445380-42445399 MS.gene007670:CDS 45.0%
AGGCTGCTATGAGGATTCAA+TGG - chr4.3:42444953-42444972 MS.gene007670:CDS 45.0%
AGTGAGGGCTGAAGATGTTA+TGG - chr4.3:42444834-42444853 MS.gene007670:CDS 45.0%
ATGGTCATCTCTGGTGTTTG+TGG - chr4.3:42445224-42445243 MS.gene007670:CDS 45.0%
CAATGCTCCTGCATGTATAC+AGG - chr4.3:42445948-42445967 MS.gene007670:intron 45.0%
CAGGCAGCAAAAATGTGAGA+GGG - chr4.3:42446963-42446982 MS.gene007670:CDS 45.0%
CCCATGTAACCTGTAGTAGT+AGG + chr4.3:42445711-42445730 None:intergenic 45.0%
CTTGGTTATGATGAAGGTGG+TGG - chr4.3:42444292-42444311 MS.gene007670:intron 45.0%
GAGCTTGGTTATGATGAAGG+TGG - chr4.3:42444289-42444308 MS.gene007670:intron 45.0%
GCCTACTACTACAGGTTACA+TGG - chr4.3:42445707-42445726 MS.gene007670:intron 45.0%
GCTGAGTTTAGTTGGCAACT+TGG - chr4.3:42446363-42446382 MS.gene007670:CDS 45.0%
GGAACTCACAAAGGTGAACA+TGG - chr4.3:42447100-42447119 MS.gene007670:CDS 45.0%
GGAATTCGTAGGGCGAAAAA+GGG - chr4.3:42444643-42444662 MS.gene007670:CDS 45.0%
GGGAACAATTGCTTCTGTTC+AGG - chr4.3:42445041-42445060 MS.gene007670:CDS 45.0%
GGTATTTGGATCCACAGCTA+TGG - chr4.3:42444050-42444069 MS.gene007670:CDS 45.0%
TCAGGCAGCAAAAATGTGAG+AGG - chr4.3:42446962-42446981 MS.gene007670:CDS 45.0%
TCTGATGCAAACAATGGTGG+GGG - chr4.3:42444373-42444392 MS.gene007670:intron 45.0%
TGACGGAAACTGAACATCGA+GGG + chr4.3:42445872-42445891 None:intergenic 45.0%
TGGAATTCGTAGGGCGAAAA+AGG - chr4.3:42444642-42444661 MS.gene007670:CDS 45.0%
TGGAGTCAGAAGATGTTGGA+AGG - chr4.3:42446415-42446434 MS.gene007670:CDS 45.0%
TGTTAAGGCTTCAGCTGTGA+AGG - chr4.3:42444933-42444952 MS.gene007670:CDS 45.0%
! ACAGGTGCTGTGAAGCAAAT+CGG - chr4.3:42446549-42446568 MS.gene007670:CDS 45.0%
! CCTTTGAGGAATTCAGAGCT+TGG - chr4.3:42444274-42444293 MS.gene007670:intron 45.0%
! GATGCATTTTCAGCTGGGAA+AGG - chr4.3:42446330-42446349 MS.gene007670:CDS 45.0%
! TCATCTTGTGGATGGACTTC+AGG - chr4.3:42444688-42444707 MS.gene007670:CDS 45.0%
!! AACCAGAAGAAGCTTGTTGC+TGG - chr4.3:42444574-42444593 MS.gene007670:intron 45.0%
!! ACGGTGAGCTTTGGAAGTTT+AGG - chr4.3:42444491-42444510 MS.gene007670:intron 45.0%
!! CACTCCCGAGTTTTGTGTTA+AGG - chr4.3:42444918-42444937 MS.gene007670:CDS 45.0%
!! CCAAGCTCTGAATTCCTCAA+AGG + chr4.3:42444277-42444296 None:intergenic 45.0%
!! GTGTTTTTGAGGGCTGAGAA+TGG - chr4.3:42444607-42444626 MS.gene007670:intron 45.0%
!! TTCAATGGTGTTCTGGGATG+AGG - chr4.3:42444968-42444987 MS.gene007670:CDS 45.0%
ACCCTGTTGTAAGCAAGTGC+CGG + chr4.3:42444540-42444559 None:intergenic 50.0%
ACGGAAACTGAACATCGAGG+GGG + chr4.3:42445870-42445889 None:intergenic 50.0%
AGCTGGTTGCCTGAAAACGT+CGG + chr4.3:42445895-42445914 None:intergenic 50.0%
AGGTTGTTGATCCTATCCGC+TGG - chr4.3:42445061-42445080 MS.gene007670:CDS 50.0%
ATCGTCTCAGCACAGTACCT+TGG + chr4.3:42444409-42444428 None:intergenic 50.0%
CACCAGCAACAAGCTTCTTC+TGG + chr4.3:42444579-42444598 None:intergenic 50.0%
CGTAGGGCGAAAAAGGGAAT+CGG - chr4.3:42444649-42444668 MS.gene007670:CDS 50.0%
GACGGAAACTGAACATCGAG+GGG + chr4.3:42445871-42445890 None:intergenic 50.0%
GAGTGGAAGAGTGAGAGTGA+GGG - chr4.3:42444819-42444838 MS.gene007670:CDS 50.0%
GCATGTTCAGCATGTCCTTG+TGG + chr4.3:42444136-42444155 None:intergenic 50.0%
GTGCTGAGACGATCTTTCCA+AGG - chr4.3:42444416-42444435 MS.gene007670:intron 50.0%
TCACAGCACCTGTTGCATCA+CGG + chr4.3:42446542-42446561 None:intergenic 50.0%
TCAGCTGCGTAATCAAGCCT+TGG + chr4.3:42444436-42444455 None:intergenic 50.0%
TCCTCCAAATTGAGCATGCC+TGG + chr4.3:42445977-42445996 None:intergenic 50.0%
TCTTCCTCGGTGGAGTGAAA+GGG + chr4.3:42445819-42445838 None:intergenic 50.0%
TGAGTGGAAGAGTGAGAGTG+AGG - chr4.3:42444818-42444837 MS.gene007670:CDS 50.0%
TTCTTCCTCGGTGGAGTGAA+AGG + chr4.3:42445820-42445839 None:intergenic 50.0%
TTGCCTGAAAACGTCGGTGA+CGG + chr4.3:42445889-42445908 None:intergenic 50.0%
! CTGATGCAAACAATGGTGGG+GGG - chr4.3:42444374-42444393 MS.gene007670:intron 50.0%
! GCTTGTTGCTGGTGATTCTG+TGG - chr4.3:42444585-42444604 MS.gene007670:intron 50.0%
! GGTTATGATGAAGGTGGTGG+TGG - chr4.3:42444295-42444314 MS.gene007670:intron 50.0%
! GTGACGGAAACTGAACATCG+AGG + chr4.3:42445873-42445892 None:intergenic 50.0%
! TGTGGATGGACTTCAGGTTC+TGG - chr4.3:42444694-42444713 MS.gene007670:CDS 50.0%
! TTTCCGTCACCGACGTTTTC+AGG - chr4.3:42445883-42445902 MS.gene007670:intron 50.0%
!! AATACCTGAAGAAGGCGCCA+CGG + chr4.3:42445096-42445115 None:intergenic 50.0%
!! ACGTTTTCAGGCAACCAGCT+CGG - chr4.3:42445895-42445914 MS.gene007670:intron 50.0%
!!! GGAATCGGTGGTGGTTTTGA+TGG - chr4.3:42444664-42444683 MS.gene007670:CDS 50.0%
AGGATGTGCACGGTGAGCTT+TGG - chr4.3:42444482-42444501 MS.gene007670:intron 55.0%
AGGGCGAAAAAGGGAATCGG+TGG - chr4.3:42444652-42444671 MS.gene007670:CDS 55.0%
CAGCTATGGCATGCTTGTGC+TGG - chr4.3:42444064-42444083 MS.gene007670:CDS 55.0%
CAGCTGTGAAGGCTGCTATG+AGG - chr4.3:42444944-42444963 MS.gene007670:CDS 55.0%
CTATGGCATGCTTGTGCTGG+TGG - chr4.3:42444067-42444086 MS.gene007670:CDS 55.0%
CTGCATGTATACAGGGAGCC+AGG - chr4.3:42445956-42445975 MS.gene007670:intron 55.0%
CTGGCTCCCTGTATACATGC+AGG + chr4.3:42445958-42445977 None:intergenic 55.0%
GCACAAGCATGCCATAGCTG+TGG + chr4.3:42444064-42444083 None:intergenic 55.0%
GCCAGGCATGCTCAATTTGG+AGG - chr4.3:42445973-42445992 MS.gene007670:intron 55.0%
GCCGGCACTTGCTTACAACA+GGG - chr4.3:42444536-42444555 MS.gene007670:intron 55.0%
GCGAAAAAGGGAATCGGTGG+TGG - chr4.3:42444655-42444674 MS.gene007670:CDS 55.0%
GGAAACCGCAGCTTCTTCCT+CGG + chr4.3:42445832-42445851 None:intergenic 55.0%
GGAGAATTAGGCCAGCGGAT+AGG + chr4.3:42445075-42445094 None:intergenic 55.0%
GGAGCCAGGCATGCTCAATT+TGG - chr4.3:42445970-42445989 MS.gene007670:intron 55.0%
GGCACTTGCTTACAACAGGG+TGG - chr4.3:42444539-42444558 MS.gene007670:intron 55.0%
GGCTGACCCTGAAACTGATG+AGG - chr4.3:42444228-42444247 MS.gene007670:CDS 55.0%
TTTATAGAGGGACACCGCGC+CGG - chr4.3:42444518-42444537 MS.gene007670:intron 55.0%
! GCATGCTTGTGCTGGTGGAA+TGG - chr4.3:42444072-42444091 MS.gene007670:CDS 55.0%
! GTGGGGGGTTTTCTGTTCCA+AGG - chr4.3:42444389-42444408 MS.gene007670:intron 55.0%
!! AACTCGGGAGTGCTTGCTCT+TGG + chr4.3:42444910-42444929 None:intergenic 55.0%
!! AGAAGGCGCCACGGAGAATT+AGG + chr4.3:42445087-42445106 None:intergenic 55.0%
!! AGGTGGTGGTGGTGATGGTT+TGG - chr4.3:42444306-42444325 MS.gene007670:intron 55.0%
!! CGTTTTCAGGCAACCAGCTC+GGG - chr4.3:42445896-42445915 MS.gene007670:intron 55.0%
!! GATGAAGGTGGTGGTGGTGA+TGG - chr4.3:42444301-42444320 MS.gene007670:intron 55.0%
AACCGCAGCTTCTTCCTCGG+TGG + chr4.3:42445829-42445848 None:intergenic 60.0%
ACGTGAAGCGTGTTAGCCCG+TGG - chr4.3:42445752-42445771 MS.gene007670:intron 60.0%
AGCTGAGCCGCCTGTTCAGA+CGG - chr4.3:42444450-42444469 MS.gene007670:intron 60.0%
AGGGGGAAGTCGAGGTTTGG+TGG + chr4.3:42445853-42445872 None:intergenic 60.0%
CGCCGGCACTTGCTTACAAC+AGG - chr4.3:42444535-42444554 MS.gene007670:intron 60.0%
CTCCACCGAGGAAGAAGCTG+CGG - chr4.3:42445824-42445843 MS.gene007670:intron 60.0%
GAACATCGAGGGGGAAGTCG+AGG + chr4.3:42445861-42445880 None:intergenic 60.0%
GAAGCGTGTTAGCCCGTGGT+TGG - chr4.3:42445756-42445775 MS.gene007670:intron 60.0%
GCCACGGAGAATTAGGCCAG+CGG + chr4.3:42445080-42445099 None:intergenic 60.0%
GGGCCAAACAGCCCCTTCTT+TGG - chr4.3:42445916-42445935 MS.gene007670:intron 60.0%
TCAGCCCTTTCACTCCACCG+AGG - chr4.3:42445812-42445831 MS.gene007670:intron 60.0%
TCGAGGGGGAAGTCGAGGTT+TGG + chr4.3:42445856-42445875 None:intergenic 60.0%
TTCGCCACCACCGTCTGAAC+AGG + chr4.3:42444463-42444482 None:intergenic 60.0%
TTCTCCGTGGCGCCTTCTTC+AGG - chr4.3:42445089-42445108 MS.gene007670:CDS 60.0%
! GTGGTGGCGAAGGATGTGCA+CGG - chr4.3:42444472-42444491 MS.gene007670:intron 60.0%
! TCCGCTGGCCTAATTCTCCG+TGG - chr4.3:42445076-42445095 MS.gene007670:CDS 60.0%
! TGTTCAGACGGTGGTGGCGA+AGG - chr4.3:42444462-42444481 MS.gene007670:intron 60.0%
!! GTTGTAAGCAAGTGCCGGCG+CGG + chr4.3:42444535-42444554 None:intergenic 60.0%
GCCACCACCGTCTGAACAGG+CGG + chr4.3:42444460-42444479 None:intergenic 65.0%
TGAGCCGCCTGTTCAGACGG+TGG - chr4.3:42444453-42444472 MS.gene007670:intron 65.0%
AGGGGCTGTTTGGCCCGAGC+TGG + chr4.3:42445912-42445931 None:intergenic 70.0%
GCCGCCTGTTCAGACGGTGG+TGG - chr4.3:42444456-42444475 MS.gene007670:intron 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 42444031 42447168 42444031 ID=MS.gene007670
chr4.3 mRNA 42444031 42447168 42444031 ID=MS.gene007670.t1;Parent=MS.gene007670
chr4.3 exon 42446089 42447168 42446089 ID=MS.gene007670.t1.exon1;Parent=MS.gene007670.t1
chr4.3 CDS 42446089 42447168 42446089 ID=cds.MS.gene007670.t1;Parent=MS.gene007670.t1
chr4.3 exon 42444613 42445478 42444613 ID=MS.gene007670.t1.exon2;Parent=MS.gene007670.t1
chr4.3 CDS 42444613 42445478 42444613 ID=cds.MS.gene007670.t1;Parent=MS.gene007670.t1
chr4.3 exon 42444215 42444280 42444215 ID=MS.gene007670.t1.exon3;Parent=MS.gene007670.t1
chr4.3 CDS 42444215 42444280 42444215 ID=cds.MS.gene007670.t1;Parent=MS.gene007670.t1
chr4.3 exon 42444031 42444112 42444031 ID=MS.gene007670.t1.exon4;Parent=MS.gene007670.t1
chr4.3 CDS 42444031 42444112 42444031 ID=cds.MS.gene007670.t1;Parent=MS.gene007670.t1
Gene Sequence

>MS.gene007670

ATGAAGGAAAGTGAGAAAGGGTATTTGGATCCACAGCTATGGCATGCTTGTGCTGGTGGAATGGTTCAGATACCTAAAGTGAACTCCAAAGTTTTCTACTTTCCACAAGGACATGCTGAACATGCTAATACAAACATTGATCTAAGGGTTTCAGTTTCACCACTGATTCTTTGTAAGGTTGTTGGTGTTAAATTCATGGCTGACCCTGAAACTGATGAGGTTTTTGCTGTAATGAAGCTTCTTCCTTTGAGGAATTCAGAGCTTGGTTATGATGAAGGTGGTGGTGGTGATGGTTTGGAAAATTCAGAAAAGCCTGCATCTTTTGCTAAGACTTTGACTCAATCTGATGCAAACAATGGTGGGGGGTTTTCTGTTCCAAGGTACTGTGCTGAGACGATCTTTCCAAGGCTTGATTACGCAGCTGAGCCGCCTGTTCAGACGGTGGTGGCGAAGGATGTGCACGGTGAGCTTTGGAAGTTTAGGCATATTTATAGAGGGACACCGCGCCGGCACTTGCTTACAACAGGGTGGAGTAGTTTTGTGAACCAGAAGAAGCTTGTTGCTGGTGATTCTGTGGTGTTTTTGAGGGCTGAGAATGGTGAATTGTGTGTTGGAATTCGTAGGGCGAAAAAGGGAATCGGTGGTGGTTTTGATGGTTCATCTTGTGGATGGACTTCAGGTTCTGGTTCTAACAATGTAAATTGTGGAATTGGACCTTTTGGAGCATTTTCTTTTTTCTTGAAAGAAGAGAATAAGACATTGAGAAATGGTTGTTTTGATGGTAATTTGAGTGGAAGAGTGAGAGTGAGGGCTGAAGATGTTATGGAAGCTGTGAAATTGGCTGCTAATAACCAACCATTTGAAGTTGTTTACTATCCAAGAGCAAGCACTCCCGAGTTTTGTGTTAAGGCTTCAGCTGTGAAGGCTGCTATGAGGATTCAATGGTGTTCTGGGATGAGGTTCAAGATGCCCTTTGAAACTGAAGACTCTTCTAGGATTAGTTGGTTTATGGGAACAATTGCTTCTGTTCAGGTTGTTGATCCTATCCGCTGGCCTAATTCTCCGTGGCGCCTTCTTCAGGTATTGTTTTTCAGTTACAATTTGGAGAAAATATGTTGAACACATTTTTACATAAGGGACTCAATTTTATGCTTCTTTGGATATCTATCTGTGCTTTGTTATTGTTCAGTTTTATGGTCATCTCTGGTGTTTGTGGTTATATGGTGTAAATATCTGTAAAAACAAGTCGATTTAGCTGAAATCATAGGTATAATTTTTCAATCAACCTCCTATACTACTAATTGTTTCATTTATTCGTGCACTAATTCAAGTGAAATTCTTGAAAAAATAGCTCAATTGTCTTACTGCGAGGTTTTAAATTGCAGTAGCATTGCTAGTCGTGATATTGTGGATAATCACAGGCAAATGTGGCTATTACGCCTCAGTTGCGATCTCAAAGATATCTAAATCCTTGATATTATTGCCCAGATTGCGGGGTGAACTTTTTTCAAACTCTTGGTTTTTTTTTCACTCTTGTGATTATGAGGATGAAATAACCTGTGTTAAGTAGGGAGATGTTTTTGCATTCTTGATTGCATTCACATCTTAGTTCAAAAATCAATTTAGTTTTGCACAGAATCATATCACACATGCTTGATTATCGATCTCATTGTTATGCCTACTACTACAGGTTACATGGGATGAGCCTGATTTACTACAAAACGTGAAGCGTGTTAGCCCGTGGTTGGTCGAATTAGTATCAAATGTACCAGTCATTCATTTCAGCCCTTTCACTCCACCGAGGAAGAAGCTGCGGTTTCCACCAAACCTCGACTTCCCCCTCGATGTTCAGTTTCCGTCACCGACGTTTTCAGGCAACCAGCTCGGGCCAAACAGCCCCTTCTTTGGTTTATCTGACAATGCTCCTGCATGTATACAGGGAGCCAGGCATGCTCAATTTGGAGGAATATCTCTTTCAGATTTCAACCTTAGCAACAATAAACTTCAGTTAGGGTTGTTTCCTACTAACATTCAGCACCTTGATGTGCATAGTAGGATTTCAAATAGCAACAACATGATGAACAACAATGATAAGACCAAAGAAAGCTTATCATGCCTGTTGAGTATCGGGAAAAGATATGATAAGAGTTCAGAGAAATCTCAATCTGATGACAATGTTGTTAAGAAACATCAGTTTTGTCTTTTTGGTCAACCAATACTCACTGAGCAACAAATTTCTAGAAACTGTTCAGAAAAAAATTCAGTAGATGATAGTAAAGACAAAGAGAAGTGGTTTTTGGATGCATTTTCAGCTGGGAAAGGTTCTAATGCTGCTGAGTTTAGTTGGCAACTTGGATTGGATATTGGTCATTGCAAGGTTTTCTTGGAGTCAGAAGATGTTGGAAGGACCCTTGATTTATCACTTCTTGGTTCTTATGAAGAGCTCTACAAGAAACTTGCTAACATGTTTGATTTAGAAAAGTCTGAGATGTTGAATCATGTTTTTTACCGTGATGCAACAGGTGCTGTGAAGCAAATCGGTGAAGAACCCTTCAGGTATTGCTTTTGTGTTTTGAGTAAACAAGCGCCTTGGTCCTTGAATATGTCAGACGGTGTTACTATAGTCTCTGAATGTATCAAAATTACAAAAACATATCTAAATGTGTCTTTTGCTAGTTTTTTTGGTCCGGTTGTTAGTTTAGTCTACAAAATTACTATGTTTTTTTAAGTTTCACTATACATTCTAGGACTATGATGACACTGACACGCACGTTTAAAGACCAAAATGACTTTACTCTATTTTTTTTCTTTTGAATTTTCCCATTATTGTACAGTAGCAAAATAGTATTACTCTTGGATTAATGGGGGGTTTTGTGATCTCATTGCAGTGAATTCATGAAGACAGCTAAAAGATTGACAATTCTGACAGATTCAGGCAGCAAAAATGTGAGAGGGTAAGAGAGAAAATGAGTTCATCAGTTTTGTTTTATTCATCTGTGTAAATGATTATGTGTTGAAATGTCTTATATAATAAGAGGATCTTGATTGTTTTTCAGGGCATGGATAACAGGAACTCACAAAGGTGAACATGGATTGGATGCATCAAACAAAACAGGACCTCTAAGCATATTTGCATAA
Protein sequence

>MS.gene007670.t1

MKESEKGYLDPQLWHACAGGMVQIPKVNSKVFYFPQGHAEHANTNIDLRVSVSPLILCKVVGVKFMADPETDEVFAVMKLLPLRNSELGYDEGGGGDGLENSEKPASFAKTLTQSDANNGGGFSVPRYCAETIFPRLDYAAEPPVQTVVAKDVHGELWKFRHIYRGTPRRHLLTTGWSSFVNQKKLVAGDSVVFLRAENGELCVGIRRAKKGIGGGFDGSSCGWTSGSGSNNVNCGIGPFGAFSFFLKEENKTLRNGCFDGNLSGRVRVRAEDVMEAVKLAANNQPFEVVYYPRASTPEFCVKASAVKAAMRIQWCSGMRFKMPFETEDSSRISWFMGTIASVQVVDPIRWPNSPWRLLQVTWDEPDLLQNVKRVSPWLVELVSNVPVIHFSPFTPPRKKLRFPPNLDFPLDVQFPSPTFSGNQLGPNSPFFGLSDNAPACIQGARHAQFGGISLSDFNLSNNKLQLGLFPTNIQHLDVHSRISNSNNMMNNNDKTKESLSCLLSIGKRYDKSSEKSQSDDNVVKKHQFCLFGQPILTEQQISRNCSEKNSVDDSKDKEKWFLDAFSAGKGSNAAEFSWQLGLDIGHCKVFLESEDVGRTLDLSLLGSYEELYKKLANMFDLEKSEMLNHVFYRDATGAVKQIGEEPFSEFMKTAKRLTILTDSGSKNVRGAWITGTHKGEHGLDASNKTGPLSIFA