AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene008629

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008629.t1 MTR_3g031770 95.445 461 21 0 1 461 1 461 0.0 893
MS.gene008629.t1 MTR_2g027560 47.196 428 222 2 26 449 27 454 7.12e-140 411
MS.gene008629.t1 MTR_1g010120 43.871 465 252 6 12 467 9 473 3.32e-134 396
MS.gene008629.t1 MTR_4g124440 43.167 461 250 6 6 455 2 461 5.33e-124 370
MS.gene008629.t1 MTR_4g112025 39.565 460 270 6 7 458 11 470 2.12e-118 357
MS.gene008629.t1 MTR_1g053320 41.522 460 258 8 9 459 14 471 2.64e-118 356
MS.gene008629.t1 MTR_3g108600 43.207 449 230 7 13 456 18 446 3.81e-118 355
MS.gene008629.t1 MTR_7g085390 44.394 437 235 7 25 454 24 459 5.38e-117 352
MS.gene008629.t1 MTR_3g095050 42.466 438 241 9 26 456 33 466 1.60e-114 346
MS.gene008629.t1 MTR_8g092070 39.387 457 266 6 26 477 33 483 1.05e-113 347
MS.gene008629.t1 MTR_6g032820 44.111 433 231 8 7 429 6 437 7.89e-113 340
MS.gene008629.t1 MTR_4g134280 46.041 341 176 3 115 449 2 340 6.50e-106 320
MS.gene008629.t1 MTR_4g088155 32.584 445 266 17 27 446 23 458 5.89e-64 214
MS.gene008629.t1 MTR_6g088885 30.556 468 271 17 14 445 5 454 7.39e-60 204
MS.gene008629.t1 MTR_1g031900 34.240 441 249 15 27 445 24 445 9.85e-59 201
MS.gene008629.t1 MTR_1g031900 34.240 441 249 15 27 445 24 445 1.26e-58 201
MS.gene008629.t1 MTR_5g085580 31.929 451 268 14 27 446 25 467 1.80e-57 197
MS.gene008629.t1 MTR_1g056640 29.870 462 276 13 26 446 37 491 2.21e-57 197
MS.gene008629.t1 MTR_8g085720 32.192 438 265 13 27 446 26 449 8.29e-57 196
MS.gene008629.t1 MTR_4g132280 30.531 452 267 14 27 453 45 474 1.73e-54 191
MS.gene008629.t1 MTR_4g069940 30.349 458 291 13 6 446 14 460 2.58e-54 189
MS.gene008629.t1 MTR_8g012400 29.598 473 292 18 1 446 1 459 1.16e-53 187
MS.gene008629.t1 MTR_3g116510 30.599 451 291 13 12 445 9 454 1.25e-53 187
MS.gene008629.t1 MTR_3g065460 30.041 486 297 15 12 472 5 472 4.08e-53 186
MS.gene008629.t1 MTR_5g078200 29.787 470 279 15 13 453 5 452 2.14e-51 184
MS.gene008629.t1 MTR_7g053500 28.571 441 285 16 28 446 27 459 3.23e-50 178
MS.gene008629.t1 MTR_3g083580 30.044 456 280 16 11 445 9 446 7.84e-50 177
MS.gene008629.t1 MTR_7g026340 30.921 456 258 23 22 445 18 448 3.87e-48 172
MS.gene008629.t1 MTR_5g078200 29.885 435 264 14 41 453 4 419 8.33e-48 174
MS.gene008629.t1 MTR_1g052430 31.677 322 209 7 24 342 29 342 6.98e-47 167
MS.gene008629.t1 MTR_4g076570 31.056 322 211 7 24 342 19 332 7.69e-47 166
MS.gene008629.t1 MTR_8g102340 31.884 414 225 19 70 445 24 418 2.61e-46 167
MS.gene008629.t1 MTR_4g010200 32.295 353 223 9 1 342 3 350 6.63e-46 164
MS.gene008629.t1 MTR_4g076255 32.219 329 191 11 24 342 22 328 7.40e-46 162
MS.gene008629.t1 MTR_4g076470 31.471 340 212 11 8 341 1 325 1.72e-45 162
MS.gene008629.t1 MTR_2g034440 29.846 325 219 6 19 341 14 331 4.62e-45 160
MS.gene008629.t1 MTR_4g076490 34.579 321 189 11 27 342 97 401 6.11e-45 162
MS.gene008629.t1 MTR_4g083500 28.541 473 291 15 21 458 38 498 1.80e-44 164
MS.gene008629.t1 MTR_4g076440 30.792 341 215 12 8 342 1 326 2.95e-44 158
MS.gene008629.t1 MTR_4g076430 31.378 341 213 12 8 342 1 326 1.04e-43 157
MS.gene008629.t1 MTR_4g114850 30.571 350 228 9 19 358 18 362 1.43e-43 158
MS.gene008629.t1 MTR_4g076500 30.960 323 208 9 24 342 75 386 1.96e-43 158
MS.gene008629.t1 MTR_4g114850 31.118 331 216 8 19 342 18 343 3.49e-43 156
MS.gene008629.t1 MTR_2g034480 30.383 339 225 8 10 345 10 340 5.13e-43 156
MS.gene008629.t1 MTR_4g114850 31.118 331 216 8 19 342 18 343 5.24e-43 156
MS.gene008629.t1 MTR_4g084480 31.445 353 225 10 7 350 5 349 5.93e-43 156
MS.gene008629.t1 MTR_4g084480 31.445 353 225 10 7 350 12 356 8.36e-43 156
MS.gene008629.t1 MTR_4g084480 31.445 353 225 10 7 350 12 356 9.53e-43 156
MS.gene008629.t1 MTR_2g034470 30.178 338 226 7 24 359 27 356 2.53e-42 154
MS.gene008629.t1 MTR_3g080410 33.841 328 197 10 26 342 25 343 1.11e-41 152
MS.gene008629.t1 MTR_7g081350 30.816 331 210 10 13 335 9 328 6.78e-41 149
MS.gene008629.t1 MTR_1g022160 27.851 456 287 13 27 445 24 474 1.68e-40 152
MS.gene008629.t1 MTR_1g022160 27.632 456 288 13 27 445 24 474 4.33e-40 150
MS.gene008629.t1 MTR_2g034470 30.094 319 213 7 43 359 11 321 2.32e-39 145
MS.gene008629.t1 MTR_7g053500 28.278 389 249 16 80 446 2 382 1.01e-38 145
MS.gene008629.t1 MTR_1g022160 27.137 468 292 14 27 457 24 479 1.15e-38 147
MS.gene008629.t1 MTR_4g052970 30.211 331 213 11 26 347 28 349 3.02e-38 144
MS.gene008629.t1 MTR_8g012400 28.316 392 242 17 80 446 2 379 1.07e-35 136
MS.gene008629.t1 MTR_7g081370 29.042 334 209 10 5 335 8 316 1.58e-35 135
MS.gene008629.t1 MTR_1g007810 32.622 328 198 13 17 332 4 320 1.90e-35 135
MS.gene008629.t1 MTR_2g019345 29.683 347 226 11 7 344 4 341 1.46e-33 131
MS.gene008629.t1 MTR_3g116510 30.218 321 208 9 138 445 3 320 7.22e-33 127
MS.gene008629.t1 MTR_1g022160 28.963 328 220 8 27 346 24 346 1.42e-31 125
MS.gene008629.t1 MTR_2g086530 28.492 358 226 13 3 344 2 345 1.69e-31 125
MS.gene008629.t1 MTR_1g077990 28.701 331 220 10 20 342 24 346 3.98e-31 124
MS.gene008629.t1 MTR_2g086530 29.091 330 207 12 28 344 9 324 1.11e-30 122
MS.gene008629.t1 MTR_5g081720 27.019 322 214 12 43 354 53 363 2.31e-29 119
MS.gene008629.t1 MTR_8g085440 45.631 103 42 5 351 446 21 116 2.54e-17 78.2
MS.gene008629.t1 MTR_8g085420 44.792 96 39 5 351 439 21 109 1.19e-13 68.6
MS.gene008629.t1 MTR_4g036680 35.714 98 57 3 247 342 82 175 1.79e-13 70.1
MS.gene008629.t1 MTR_4g036775 35.714 98 57 3 247 342 82 175 4.79e-13 68.9
MS.gene008629.t1 MTR_8g085390 43.617 94 49 4 356 446 22 114 2.92e-12 65.9
MS.gene008629.t1 MTR_4g079530 37.113 97 56 4 364 456 37 132 3.06e-11 62.4
MS.gene008629.t1 MTR_5g091650 37.363 91 47 4 362 446 106 192 9.33e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008629.t1 AT5G58480 67.593 432 136 2 25 454 24 453 0.0 604
MS.gene008629.t1 AT4G17180 44.124 451 240 5 4 449 1 444 2.60e-142 417
MS.gene008629.t1 AT4G31140 42.826 453 251 5 9 454 6 457 3.11e-128 381
MS.gene008629.t1 AT5G20870 43.312 471 251 7 1 456 1 470 1.38e-127 380
MS.gene008629.t1 AT5G58090 42.795 458 257 5 24 476 19 476 5.80e-126 375
MS.gene008629.t1 AT3G24330 42.737 475 259 8 14 476 22 495 1.35e-124 372
MS.gene008629.t1 AT5G64790 41.556 450 257 6 15 459 16 464 1.00e-122 367
MS.gene008629.t1 AT2G19440 45.249 442 232 9 15 449 10 448 1.10e-120 362
MS.gene008629.t1 AT2G19440 45.249 442 232 9 15 449 10 448 1.10e-120 362
MS.gene008629.t1 AT1G64760 43.312 471 258 8 13 476 11 479 1.08e-119 359
MS.gene008629.t1 AT1G64760 43.312 471 258 8 13 476 11 479 1.08e-119 359
MS.gene008629.t1 AT3G04010 41.845 466 255 10 20 473 26 487 1.06e-118 357
MS.gene008629.t1 AT5G18220 41.458 480 255 12 14 472 14 488 3.17e-115 348
MS.gene008629.t1 AT4G26830 32.166 457 285 13 8 446 3 452 4.67e-66 219
MS.gene008629.t1 AT5G55180 33.559 444 264 15 24 446 24 457 3.42e-65 218
MS.gene008629.t1 AT4G26830 32.653 441 275 12 21 446 23 456 6.28e-65 217
MS.gene008629.t1 AT5G55180 33.638 437 259 15 24 439 24 450 3.17e-62 210
MS.gene008629.t1 AT4G14080 30.689 479 282 16 10 446 4 474 9.94e-62 209
MS.gene008629.t1 AT3G23770 30.752 452 281 16 19 446 29 472 2.74e-61 207
MS.gene008629.t1 AT2G01630 34.163 442 250 15 27 446 23 445 4.10e-61 208
MS.gene008629.t1 AT5G56590 31.839 446 259 14 27 445 26 453 5.37e-60 205
MS.gene008629.t1 AT2G01630 34.023 435 246 15 27 439 23 438 2.63e-59 201
MS.gene008629.t1 AT3G07320 31.121 437 277 15 28 446 26 456 7.83e-57 195
MS.gene008629.t1 AT2G05790 31.126 453 273 13 26 446 24 469 9.01e-57 196
MS.gene008629.t1 AT1G66250 32.045 440 259 15 27 445 32 452 1.37e-56 196
MS.gene008629.t1 AT4G34480 32.230 453 282 13 8 446 7 448 2.04e-56 195
MS.gene008629.t1 AT1G11820 30.317 442 267 14 27 446 44 466 2.16e-55 193
MS.gene008629.t1 AT1G11820 30.115 435 263 14 27 439 44 459 5.50e-53 186
MS.gene008629.t1 AT5G24318 31.102 463 276 19 12 445 7 455 2.17e-51 181
MS.gene008629.t1 AT5G24318 31.291 457 271 19 12 439 7 449 3.21e-50 177
MS.gene008629.t1 AT3G55430 30.243 453 268 20 18 445 20 449 6.10e-50 177
MS.gene008629.t1 AT3G57260 32.710 321 199 7 27 344 32 338 2.90e-49 172
MS.gene008629.t1 AT2G16230 30.155 451 259 18 27 446 25 450 3.28e-49 176
MS.gene008629.t1 AT3G57260 32.710 321 199 7 27 344 48 354 7.34e-49 171
MS.gene008629.t1 AT3G13560 32.723 437 260 13 27 445 26 446 8.11e-49 175
MS.gene008629.t1 AT3G13560 32.723 437 260 13 27 445 26 446 8.11e-49 175
MS.gene008629.t1 AT3G13560 32.723 437 260 13 27 445 26 446 8.11e-49 175
MS.gene008629.t1 AT3G13560 32.723 437 260 13 27 445 26 446 8.11e-49 175
MS.gene008629.t1 AT3G13560 32.723 437 260 13 27 445 26 446 8.11e-49 175
MS.gene008629.t1 AT2G39640 29.605 456 295 20 3 447 4 444 8.28e-48 173
MS.gene008629.t1 AT4G29360 26.452 465 300 11 23 451 33 491 1.48e-47 172
MS.gene008629.t1 AT4G29360 26.452 465 300 11 23 451 23 481 1.66e-47 172
MS.gene008629.t1 AT3G57270 31.153 321 210 7 27 344 27 339 4.82e-46 163
MS.gene008629.t1 AT2G01630 32.718 379 218 13 27 389 23 380 4.44e-43 157
MS.gene008629.t1 AT4G16260 28.909 339 227 8 7 342 5 332 2.52e-42 154
MS.gene008629.t1 AT4G34480 32.955 352 220 9 8 350 7 351 1.18e-41 152
MS.gene008629.t1 AT2G26600 31.481 324 206 9 28 342 35 351 4.96e-40 149
MS.gene008629.t1 AT3G57240 32.087 321 200 8 27 344 35 340 6.29e-40 147
MS.gene008629.t1 AT3G55780 29.377 337 215 9 27 349 31 358 9.24e-40 147
MS.gene008629.t1 AT3G15800 31.288 326 209 8 28 344 44 363 2.29e-39 147
MS.gene008629.t1 AT3G61810 29.607 331 210 8 24 343 52 370 2.70e-38 144
MS.gene008629.t1 AT4G16260 29.236 301 200 7 45 342 13 303 5.58e-38 141
MS.gene008629.t1 AT5G24318 30.504 377 228 16 12 368 7 369 1.26e-37 142
MS.gene008629.t1 AT5G42720 31.778 343 217 9 12 344 11 346 9.14e-37 140
MS.gene008629.t1 AT1G11820 30.435 322 196 10 27 332 44 353 1.26e-35 136
MS.gene008629.t1 AT4G18340 27.746 346 233 10 26 362 33 370 5.19e-35 135
MS.gene008629.t1 AT4G18340 27.746 346 233 10 26 362 33 370 1.13e-34 134
MS.gene008629.t1 AT1G30080 29.448 326 213 10 26 342 35 352 8.60e-34 131
MS.gene008629.t1 AT4G34480 31.889 323 197 11 138 446 24 337 1.80e-33 130
MS.gene008629.t1 AT1G30080 29.448 326 213 10 26 342 35 352 2.17e-33 130
MS.gene008629.t1 AT5G20340 27.707 314 211 9 24 332 40 342 6.18e-33 128
MS.gene008629.t1 AT1G33220 28.333 300 202 8 24 318 31 322 4.07e-32 125
MS.gene008629.t1 AT2G27500 27.711 332 223 10 25 347 27 350 5.30e-32 126
MS.gene008629.t1 AT2G27500 27.711 332 223 10 25 347 27 350 7.23e-32 126
MS.gene008629.t1 AT5G20560 27.356 329 222 10 11 332 16 334 8.03e-32 125
MS.gene008629.t1 AT5G24318 29.630 324 199 12 141 445 3 316 9.94e-32 124
MS.gene008629.t1 AT5G42100 30.000 340 222 11 15 344 15 348 4.86e-31 124
MS.gene008629.t1 AT5G42100 30.000 340 222 11 15 344 15 348 4.87e-31 124
MS.gene008629.t1 AT3G46570 29.023 348 227 12 12 349 12 349 1.24e-30 122
MS.gene008629.t1 AT5G20390 25.321 312 215 8 27 332 33 332 3.30e-29 117
MS.gene008629.t1 AT1G32860 28.049 328 217 11 26 342 28 347 5.89e-29 119
MS.gene008629.t1 AT5G20330 27.389 314 212 9 24 332 31 333 8.08e-29 117
MS.gene008629.t1 AT1G77780 25.000 332 232 9 7 332 2 322 2.23e-26 110
MS.gene008629.t1 AT2G26600 29.167 264 171 9 88 342 1 257 2.08e-23 100
MS.gene008629.t1 AT1G77790 25.225 333 232 10 17 342 19 341 2.33e-23 101
MS.gene008629.t1 AT2G27500 25.448 279 191 10 78 347 2 272 1.94e-17 83.6
MS.gene008629.t1 AT5G63225 35.556 90 46 4 361 443 22 106 1.60e-11 61.6
MS.gene008629.t1 AT1G66870 34.343 99 62 2 350 445 39 137 3.19e-11 61.6
MS.gene008629.t1 AT1G66870 34.343 99 62 2 350 445 12 110 5.69e-11 60.1

Find 103 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGTTCTTCAAGCTCTTTC+TGG 0.110215 3.4:+60716821 MS.gene008629:CDS
CATCTAATGCAGAACTTTAC+AGG 0.219916 3.4:+60720116 MS.gene008629:CDS
AACAACCCTCTTGGCATCTT+TGG 0.240136 3.4:+60720171 MS.gene008629:CDS
GAAACAACGATTTCCATATT+AGG 0.260145 3.4:-60719536 None:intergenic
TGCTTTAAAGAAAGCAAAAT+TGG 0.261631 3.4:+60719196 MS.gene008629:CDS
TGCAACTGCAAAAGCTTTAG+AGG 0.278675 3.4:+60719889 MS.gene008629:CDS
AAGCTGAAAGTTGCGACTTT+GGG 0.293598 3.4:+60720026 MS.gene008629:CDS
AAAAGTAGGATTTCCTAATA+TGG 0.301102 3.4:+60719523 MS.gene008629:CDS
GATAGAGGATATGGTGAAAA+TGG 0.307093 3.4:-60716678 None:intergenic
CAAAAGCTTCACTACTTTCA+TGG 0.308545 3.4:-60716753 None:intergenic
ATGTTCTTCAAGCTCTTTCT+GGG 0.334463 3.4:+60716822 MS.gene008629:CDS
ATCCATCAGATGATCGTTGC+AGG 0.343975 3.4:+60720074 MS.gene008629:CDS
CTAGAAAAGTTGCTGATACT+TGG 0.346987 3.4:+60716903 MS.gene008629:CDS
TTTCACTTTGTTGATGTTGT+TGG 0.356479 3.4:-60716780 None:intergenic
TAGGCCTTTCATGAAGGTTT+CGG 0.360341 3.4:-60719609 None:intergenic
GTTACTCGCTATGTTTCTAA+TGG 0.362830 3.4:+60716938 MS.gene008629:CDS
TCAAAATACCACGTGGATCT+TGG 0.373693 3.4:+60719776 MS.gene008629:CDS
GAAATCGTTGTTTCAAAGAT+TGG 0.377594 3.4:+60719545 MS.gene008629:CDS
CTGAAGCCATAGGTGTGAAC+TGG 0.381152 3.4:+60716702 MS.gene008629:CDS
AAAGCTGAAAGTTGCGACTT+TGG 0.385336 3.4:+60720025 MS.gene008629:CDS
GGCTTCAGAGATAGAGGATA+TGG 0.386431 3.4:-60716687 None:intergenic
TGGAGATTTCGAAAGGCATT+GGG 0.400392 3.4:+60719730 MS.gene008629:CDS
CGTCCACCGAAGCGCATCTC+AGG 0.423281 3.4:+60719279 MS.gene008629:CDS
GTCTGAAGGAAAGTTAGAAA+TGG 0.424998 3.4:-60719380 None:intergenic
TGGGGTTTATTCACCTTTGA+CGG 0.427131 3.4:+60719749 MS.gene008629:CDS
GAAAGAGCTTGAAGAACATC+TGG 0.437404 3.4:-60716818 None:intergenic
GAAAGTTAGAAATGGAGAAA+TGG 0.443322 3.4:-60719372 None:intergenic
TTGGAGATTTCGAAAGGCAT+TGG 0.444933 3.4:+60719729 MS.gene008629:CDS
GAACCATTTCTTAAAAGTTA+CGG 0.448648 3.4:+60719125 MS.gene008629:CDS
TAGAAAAGTTGCTGATACTT+GGG 0.449639 3.4:+60716904 MS.gene008629:CDS
TGCTATGAAGGTGGTTCGTT+AGG 0.450346 3.4:-60719628 None:intergenic
AACCCTCTTGGCATCTTTGG+AGG 0.454084 3.4:+60720174 MS.gene008629:CDS
GAAGAAGGTGAATGTGATGA+AGG 0.457940 3.4:-60716648 None:intergenic
ACCTATGGCTTCAGAGATAG+AGG 0.458655 3.4:-60716693 None:intergenic
AAAGCATATGATATATTACT+TGG 0.472673 3.4:-60719974 None:intergenic
GGTTCCCCAGTTCACACCTA+TGG 0.476387 3.4:-60716708 None:intergenic
GGAGAAATGGTCACAAAGAA+AGG 0.481696 3.4:-60719359 None:intergenic
ACTACTTTCATGGGTGCTAG+TGG 0.486222 3.4:-60716743 None:intergenic
GTTCGTTAGGCCTTTCATGA+AGG 0.487779 3.4:-60719615 None:intergenic
ATACCACGTGGATCTTGGCC+AGG 0.491196 3.4:+60719781 MS.gene008629:CDS
AAAAGCTTCACTACTTTCAT+GGG 0.496458 3.4:-60716752 None:intergenic
CAATTCCATCCCTTCACAAT+CGG 0.497846 3.4:+60719152 MS.gene008629:CDS
ATGCGCTTCGGTGGACGAGT+TGG 0.498782 3.4:-60719273 None:intergenic
GATCAAAGGAGCATTATTGT+TGG 0.509044 3.4:+60719710 MS.gene008629:CDS
TGCTAGTGGGTGTGAAGCCA+TGG 0.511774 3.4:-60716729 None:intergenic
GATTGTACTGCACTATCTTC+CGG 0.513984 3.4:+60719926 MS.gene008629:CDS
CTGAAAGTTGCGACTTTGGG+GGG 0.514636 3.4:+60720029 MS.gene008629:CDS
TTTACTAGTAACAACCCTCT+TGG 0.516527 3.4:+60720162 MS.gene008629:CDS
TTAGATGTGTGAATCTCAAT+CGG 0.519265 3.4:-60720100 None:intergenic
CTACTTTCATGGGTGCTAGT+GGG 0.519877 3.4:-60716742 None:intergenic
GTGTTCCCAAGTTGCTATGA+AGG 0.527307 3.4:-60719640 None:intergenic
AAGATTGGTTGGCCGACCGA+TGG 0.529255 3.4:+60719560 MS.gene008629:CDS
TCGAGTATCTTTCTTCAAAG+TGG 0.533132 3.4:+60719834 MS.gene008629:CDS
GCTCTTTCTGGGTCTAACAT+TGG 0.536633 3.4:+60716833 MS.gene008629:CDS
GTCAGACCTGAGATGCGCTT+CGG 0.538256 3.4:-60719285 None:intergenic
GAACCACCTTCATAGCAACT+TGG 0.538759 3.4:+60719634 MS.gene008629:CDS
TCGTTGTTTCAAAGATTGGT+TGG 0.548740 3.4:+60719549 MS.gene008629:CDS
CTTTCATGAAGGTTTCGGCT+AGG 0.553713 3.4:-60719604 None:intergenic
TCCTTTGATCCTCGTCGAGA+AGG 0.557780 3.4:-60719697 None:intergenic
GCCTTCTCGACGAGGATCAA+AGG 0.558390 3.4:+60719696 MS.gene008629:CDS
GGCTAGGTACGAGGTTGCGT+TGG 0.562721 3.4:-60719588 None:intergenic
TGAAGCCATAGGTGTGAACT+GGG 0.562869 3.4:+60716703 MS.gene008629:CDS
CAGCTCCGATTGTGAAGGGA+TGG 0.564940 3.4:-60719157 None:intergenic
TAAATGTATGTTTCGTGTGG+TGG 0.567154 3.4:-60719671 None:intergenic
CATAGCAGCTCCGATTGTGA+AGG 0.572223 3.4:-60719162 None:intergenic
AGCTGAAAGTTGCGACTTTG+GGG 0.572621 3.4:+60720027 MS.gene008629:CDS
AACCTGCAACGATCATCTGA+TGG 0.575288 3.4:-60720076 None:intergenic
TTCCCAAGTTGCTATGAAGG+TGG 0.575432 3.4:-60719637 None:intergenic
TCTAACATTGGTGTCACTGT+TGG 0.577685 3.4:+60716845 MS.gene008629:CDS
TCACAAGGCTCTTTGAACCC+TGG 0.578006 3.4:-60719799 None:intergenic
GGTTGCGTTGGCAGATCCAT+CGG 0.580707 3.4:-60719576 None:intergenic
AGACCTGAGATGCGCTTCGG+TGG 0.586712 3.4:-60719282 None:intergenic
AACCACCTTCATAGCAACTT+GGG 0.594081 3.4:+60719635 MS.gene008629:CDS
AGGTGTGAACTGGGGAACCA+TGG 0.597334 3.4:+60716712 MS.gene008629:CDS
TCCTCTATCTCTGAAGCCAT+AGG 0.600540 3.4:+60716692 MS.gene008629:CDS
TTATTGTTGGAGATTTCGAA+AGG 0.601358 3.4:+60719723 MS.gene008629:CDS
CATTTATAGCCTTCTCGACG+AGG 0.612382 3.4:+60719688 MS.gene008629:CDS
AACCTCCAAAGATGCCAAGA+GGG 0.615636 3.4:-60720176 None:intergenic
ATAGCAGCTCCGATTGTGAA+GGG 0.619868 3.4:-60719161 None:intergenic
TACCACGTGGATCTTGGCCA+GGG 0.621241 3.4:+60719782 MS.gene008629:CDS
GATGTTAAAGCAAGATCCAC+CGG 0.621470 3.4:-60719945 None:intergenic
CTAGCCGAAACCTTCATGAA+AGG 0.624280 3.4:+60719605 MS.gene008629:CDS
ACGATCATCTGATGGATCAA+CGG 0.624728 3.4:-60720068 None:intergenic
TGTATGTTTCGTGTGGTGGT+CGG 0.627215 3.4:-60719667 None:intergenic
GCTGAAAGTTGCGACTTTGG+GGG 0.628367 3.4:+60720028 MS.gene008629:CDS
AAATGTGAGGAGCTCGACCA+TGG 0.628665 3.4:-60719318 None:intergenic
GAAGGTTTCGGCTAGGTACG+AGG 0.634304 3.4:-60719597 None:intergenic
ATGTGCAGATATGTTGCTGT+TGG 0.637030 3.4:+60719098 MS.gene008629:intron
GAAAATGGTGAAGAAGAAGA+AGG 0.639303 3.4:-60716663 None:intergenic
GCGTTGGCAGATCCATCGGT+CGG 0.639821 3.4:-60719572 None:intergenic
GATCTTGCTTTAACATCACA+TGG 0.641777 3.4:+60719951 MS.gene008629:CDS
ATCTAATGCAGAACTTTACA+GGG 0.641809 3.4:+60720117 MS.gene008629:CDS
GTTTCGTGTGGTGGTCGGTG+TGG 0.644770 3.4:-60719662 None:intergenic
GGAGATTTCGAAAGGCATTG+GGG 0.653681 3.4:+60719731 MS.gene008629:CDS
TGTACTGCACTATCTTCCGG+TGG 0.659525 3.4:+60719929 MS.gene008629:CDS
TGTGATGAAGGAAGAAACAG+TGG 0.672525 3.4:-60716636 None:intergenic
AAACCTCCAAAGATGCCAAG+AGG 0.686940 3.4:-60720177 None:intergenic
GTCTGACATCAACAAAACCA+TGG 0.694847 3.4:+60719301 MS.gene008629:CDS
CGGCCAATCAAAATACCACG+TGG 0.701867 3.4:+60719769 MS.gene008629:CDS
CGTGCTTGTCGAGAAATGTG+AGG 0.707226 3.4:-60719331 None:intergenic
CTATAAATGTATGTTTCGTG+TGG 0.708772 3.4:-60719674 None:intergenic
GAAGCCATAGGTGTGAACTG+GGG 0.765647 3.4:+60716704 MS.gene008629:CDS
AACCCTGGCCAAGATCCACG+TGG 0.771430 3.4:-60719784 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATAATGTAATTATTTTAC+AGG - chr3.4:60717912-60717931 None:intergenic 10.0%
!!! ATTGTTTGTTTTATAATTAT+GGG - chr3.4:60717426-60717445 None:intergenic 10.0%
!!! TTTTTTATTTAATATACTGT+TGG + chr3.4:60717037-60717056 MS.gene008629:intron 10.0%
!! AGAAAATATACAATTAAAAG+AGG - chr3.4:60716996-60717015 None:intergenic 15.0%
!! AGGAAATAATATGAAAATAA+AGG + chr3.4:60718450-60718469 MS.gene008629:intron 15.0%
!!! CATTGTTTGTTTTATAATTA+TGG - chr3.4:60717427-60717446 None:intergenic 15.0%
!!! TGGATCTTATTTTTATATTT+CGG - chr3.4:60718198-60718217 None:intergenic 15.0%
!!! TTGAATACAAATATTTTAGA+TGG + chr3.4:60718814-60718833 MS.gene008629:intron 15.0%
!! AAAGCATATGATATATTACT+TGG - chr3.4:60719977-60719996 None:intergenic 20.0%
!! AAATTAGTTGAACATATCTT+AGG - chr3.4:60718784-60718803 None:intergenic 20.0%
!! CATAAATAATTCTTACTGTT+AGG - chr3.4:60718104-60718123 None:intergenic 20.0%
!! CTAACAGTAAGAATTATTTA+TGG + chr3.4:60718102-60718121 MS.gene008629:intron 20.0%
!! TATAAAACAAACAATGTCAT+AGG + chr3.4:60717431-60717450 MS.gene008629:intron 20.0%
!!! AGCTATTTTTGTATGTTTTA+TGG - chr3.4:60719466-60719485 None:intergenic 20.0%
!!! ATATTTGTTGTTAACTTGTT+AGG + chr3.4:60717087-60717106 MS.gene008629:intron 20.0%
!!! CTTTTGAAAAAATTTACTCT+TGG + chr3.4:60719007-60719026 MS.gene008629:intron 20.0%
!!! TATTATGATGGTAAATTTTC+TGG + chr3.4:60717848-60717867 MS.gene008629:intron 20.0%
!!! TGCTTTTAATAGTTATTATC+AGG + chr3.4:60719991-60720010 MS.gene008629:CDS 20.0%
! AAAAGTAGGATTTCCTAATA+TGG + chr3.4:60719523-60719542 MS.gene008629:CDS 25.0%
! AAATAAGTATGCTTTCTCAA+AGG + chr3.4:60718950-60718969 MS.gene008629:intron 25.0%
! ATGTGTCAAAGATAAAATGT+AGG + chr3.4:60717522-60717541 MS.gene008629:intron 25.0%
! CAATAACAAAGATGAATTGA+TGG + chr3.4:60718547-60718566 MS.gene008629:intron 25.0%
! CTACAAAAACCTATTAAACA+CGG + chr3.4:60717244-60717263 MS.gene008629:intron 25.0%
! GAACCATTTCTTAAAAGTTA+CGG + chr3.4:60719125-60719144 MS.gene008629:CDS 25.0%
! GTCGTCAACAATAATAATTT+AGG + chr3.4:60717302-60717321 MS.gene008629:intron 25.0%
! TAAAATGTAACCAATTGTGT+TGG + chr3.4:60717337-60717356 MS.gene008629:intron 25.0%
! TACTCTTGGTTGAAAAAAAA+TGG + chr3.4:60719021-60719040 MS.gene008629:intron 25.0%
! TATAGAACAAATAATGTCAC+AGG + chr3.4:60717182-60717201 MS.gene008629:intron 25.0%
! TATGACTCACTTTACATATA+TGG + chr3.4:60719060-60719079 MS.gene008629:intron 25.0%
! TGTAGAAGTTTAATCTCATA+AGG + chr3.4:60718028-60718047 MS.gene008629:intron 25.0%
! TTTAGATGGAAACTTTAACT+TGG + chr3.4:60718828-60718847 MS.gene008629:intron 25.0%
! TTTCTCTATAGAAGAAAGAT+GGG - chr3.4:60718167-60718186 None:intergenic 25.0%
!! AAAGTCAAGTGAAATGTTTT+TGG - chr3.4:60719405-60719424 None:intergenic 25.0%
!! ATCACTGTTTTATCAAAAGT+AGG + chr3.4:60719509-60719528 MS.gene008629:CDS 25.0%
!! GGTTTGTGTTATTAATAGTT+AGG + chr3.4:60717468-60717487 MS.gene008629:intron 25.0%
!! TTTTATGGCTAAGTTTATGA+GGG - chr3.4:60719451-60719470 None:intergenic 25.0%
!! TTTTCTCTATAGAAGAAAGA+TGG - chr3.4:60718168-60718187 None:intergenic 25.0%
!!! GAGTTTAGACATATTTTGAA+TGG - chr3.4:60718351-60718370 None:intergenic 25.0%
!!! TGCTTTAAAGAAAGCAAAAT+TGG + chr3.4:60719196-60719215 MS.gene008629:CDS 25.0%
AAAAGCTTCACTACTTTCAT+GGG - chr3.4:60716755-60716774 None:intergenic 30.0%
AATGATCAGCTGACATATTT+AGG + chr3.4:60718401-60718420 MS.gene008629:intron 30.0%
AATGCAGTAGTTATGATTCA+TGG + chr3.4:60717620-60717639 MS.gene008629:intron 30.0%
ATAAAGGCTAATCATGAAAC+AGG + chr3.4:60718466-60718485 MS.gene008629:intron 30.0%
ATCTAATGCAGAACTTTACA+GGG + chr3.4:60720117-60720136 MS.gene008629:CDS 30.0%
CACGATGTAAAATCTATAGA+TGG + chr3.4:60717712-60717731 MS.gene008629:intron 30.0%
CATACTTATTTGATGCTCTA+TGG - chr3.4:60718941-60718960 None:intergenic 30.0%
CTATAAATGTATGTTTCGTG+TGG - chr3.4:60719677-60719696 None:intergenic 30.0%
CTTTCATTTGCAATGAACAT+TGG - chr3.4:60717590-60717609 None:intergenic 30.0%
GAAACAACGATTTCCATATT+AGG - chr3.4:60719539-60719558 None:intergenic 30.0%
GAAAGTTAGAAATGGAGAAA+TGG - chr3.4:60719375-60719394 None:intergenic 30.0%
TAGAAAAGTTGCTGATACTT+GGG + chr3.4:60716904-60716923 MS.gene008629:CDS 30.0%
TTAGATGTGTGAATCTCAAT+CGG - chr3.4:60720103-60720122 None:intergenic 30.0%
TTATTGTTGGAGATTTCGAA+AGG + chr3.4:60719723-60719742 MS.gene008629:CDS 30.0%
TTCTCTATAGAAGAAAGATG+GGG - chr3.4:60718166-60718185 None:intergenic 30.0%
TTTCACTTTGTTGATGTTGT+TGG - chr3.4:60716783-60716802 None:intergenic 30.0%
! AAGCTTTTGAGCAAAACATT+AGG - chr3.4:60716875-60716894 None:intergenic 30.0%
! CATCTATAGATTTTACATCG+TGG - chr3.4:60717714-60717733 None:intergenic 30.0%
! CTCTTTTCTTAGTCCATATA+AGG + chr3.4:60718738-60718757 MS.gene008629:intron 30.0%
! CTTCATTTTCACTTTCTAAG+TGG + chr3.4:60718677-60718696 MS.gene008629:intron 30.0%
! CTTCTGTAGTCTTAATCATA+GGG - chr3.4:60718512-60718531 None:intergenic 30.0%
! GAAATCGTTGTTTCAAAGAT+TGG + chr3.4:60719545-60719564 MS.gene008629:CDS 30.0%
! GTTTTATGGCTAAGTTTATG+AGG - chr3.4:60719452-60719471 None:intergenic 30.0%
! TAATGTTACTGTTTTCTCAG+TGG + chr3.4:60718887-60718906 MS.gene008629:intron 30.0%
! TGACATATTTAGGATCTGTT+TGG + chr3.4:60718411-60718430 MS.gene008629:intron 30.0%
! TTGGTTTGAGAAAATAAGGA+AGG + chr3.4:60718430-60718449 MS.gene008629:intron 30.0%
!! CTGTTTGGTTTGAGAAAATA+AGG + chr3.4:60718426-60718445 MS.gene008629:intron 30.0%
!! GGTAAATTTTCTGGAAACAA+GGG + chr3.4:60717857-60717876 MS.gene008629:intron 30.0%
!! TGGTAAATTTTCTGGAAACA+AGG + chr3.4:60717856-60717875 MS.gene008629:intron 30.0%
!! TGTTAACTTGTTAGGTTCTT+TGG + chr3.4:60717095-60717114 MS.gene008629:intron 30.0%
AATCTAGCATCTTAGGACTT+GGG - chr3.4:60718373-60718392 None:intergenic 35.0%
AATTAATCACCTTCGTGAAG+GGG + chr3.4:60717811-60717830 MS.gene008629:intron 35.0%
ACTGTTAGGTGTCAAAAATG+AGG - chr3.4:60718090-60718109 None:intergenic 35.0%
AGATGGCACACATATTATGA+TGG + chr3.4:60717836-60717855 MS.gene008629:intron 35.0%
ATGTCAGCTGATCATTTCTT+TGG - chr3.4:60718397-60718416 None:intergenic 35.0%
ATGTTCTTCAAGCTCTTTCT+GGG + chr3.4:60716822-60716841 MS.gene008629:CDS 35.0%
CAAAAGCTTCACTACTTTCA+TGG - chr3.4:60716756-60716775 None:intergenic 35.0%
CAAGAATTAGTCACTGAATG+AGG + chr3.4:60717146-60717165 MS.gene008629:intron 35.0%
CATCTAATGCAGAACTTTAC+AGG + chr3.4:60720116-60720135 MS.gene008629:CDS 35.0%
CCATATAAGGAGATAATGAC+AGG + chr3.4:60718751-60718770 MS.gene008629:intron 35.0%
CCTATGATTAAGACTACAGA+AGG + chr3.4:60718510-60718529 MS.gene008629:intron 35.0%
CCTGTCATTATCTCCTTATA+TGG - chr3.4:60718754-60718773 None:intergenic 35.0%
CTAGAAAAGTTGCTGATACT+TGG + chr3.4:60716903-60716922 MS.gene008629:CDS 35.0%
CTGTTAGGTGTCAAAAATGA+GGG - chr3.4:60718089-60718108 None:intergenic 35.0%
CTTGTATGAGCTGAGAAAAT+TGG + chr3.4:60717767-60717786 MS.gene008629:intron 35.0%
GAAAATGGTGAAGAAGAAGA+AGG - chr3.4:60716666-60716685 None:intergenic 35.0%
GAAGAGTAGATTAGTGATAC+TGG + chr3.4:60718609-60718628 MS.gene008629:intron 35.0%
GAATTAATCACCTTCGTGAA+GGG + chr3.4:60717810-60717829 MS.gene008629:intron 35.0%
GATAGAGGATATGGTGAAAA+TGG - chr3.4:60716681-60716700 None:intergenic 35.0%
GATCTTGCTTTAACATCACA+TGG + chr3.4:60719951-60719970 MS.gene008629:CDS 35.0%
GCTGCTAATTGTAATCACTT+TGG - chr3.4:60718218-60718237 None:intergenic 35.0%
GTCTGAAGGAAAGTTAGAAA+TGG - chr3.4:60719383-60719402 None:intergenic 35.0%
GTGAAAATGAAGTTGCAACA+TGG - chr3.4:60718669-60718688 None:intergenic 35.0%
TAAATGTATGTTTCGTGTGG+TGG - chr3.4:60719674-60719693 None:intergenic 35.0%
TATAAGGAGATAATGACAGG+AGG + chr3.4:60718754-60718773 MS.gene008629:intron 35.0%
TCGAGTATCTTTCTTCAAAG+TGG + chr3.4:60719834-60719853 MS.gene008629:CDS 35.0%
TGAATTAATCACCTTCGTGA+AGG + chr3.4:60717809-60717828 MS.gene008629:intron 35.0%
TGGCATGAAACAAATGTAAG+AGG + chr3.4:60717640-60717659 MS.gene008629:intron 35.0%
TGTATGTGTCCGTGTTTAAT+AGG - chr3.4:60717256-60717275 None:intergenic 35.0%
TGTTAGGTGTCAAAAATGAG+GGG - chr3.4:60718088-60718107 None:intergenic 35.0%
TTCACGAAAGAAGCAATAAC+CGG + chr3.4:60718573-60718592 MS.gene008629:intron 35.0%
TTCACTTTCTAAGTGGAGTT+GGG + chr3.4:60718684-60718703 MS.gene008629:intron 35.0%
TTTACTAGTAACAACCCTCT+TGG + chr3.4:60720162-60720181 MS.gene008629:CDS 35.0%
TTTCACTTTCTAAGTGGAGT+TGG + chr3.4:60718683-60718702 MS.gene008629:intron 35.0%
! CAATTTTCTCAGCTCATACA+AGG - chr3.4:60717769-60717788 None:intergenic 35.0%
! CCTTCTGTAGTCTTAATCAT+AGG - chr3.4:60718513-60718532 None:intergenic 35.0%
! GTTACTCGCTATGTTTCTAA+TGG + chr3.4:60716938-60716957 MS.gene008629:CDS 35.0%
! TAGGTACTGTGATAACTAAG+TGG + chr3.4:60717541-60717560 MS.gene008629:intron 35.0%
! TCGTTGTTTCAAAGATTGGT+TGG + chr3.4:60719549-60719568 MS.gene008629:CDS 35.0%
!! GATCAAAGGAGCATTATTGT+TGG + chr3.4:60719710-60719729 MS.gene008629:CDS 35.0%
!! TCGCCGTAACTTTTAAGAAA+TGG - chr3.4:60719131-60719150 None:intergenic 35.0%
!!! AGCTTTGATAGTTTTGAGTC+AGG + chr3.4:60719248-60719267 MS.gene008629:CDS 35.0%
!!! GTGAAATGTTTTTGGTCTGA+AGG - chr3.4:60719397-60719416 None:intergenic 35.0%
AAAGCTGAAAGTTGCGACTT+TGG + chr3.4:60720025-60720044 MS.gene008629:CDS 40.0%
AACCACCTTCATAGCAACTT+GGG + chr3.4:60719635-60719654 MS.gene008629:CDS 40.0%
AAGACACATCACTGTTAGCT+AGG + chr3.4:60718283-60718302 MS.gene008629:intron 40.0%
AAGCTGAAAGTTGCGACTTT+GGG + chr3.4:60720026-60720045 MS.gene008629:CDS 40.0%
AGAAGAAAGATGGGGATGAA+GGG - chr3.4:60718158-60718177 None:intergenic 40.0%
ATAGTTAGGTTTGCGAAGAG+TGG + chr3.4:60717482-60717501 MS.gene008629:intron 40.0%
ATGTGCAGATATGTTGCTGT+TGG + chr3.4:60719098-60719117 MS.gene008629:intron 40.0%
CAATCTAGCATCTTAGGACT+TGG - chr3.4:60718374-60718393 None:intergenic 40.0%
CAATTCCATCCCTTCACAAT+CGG + chr3.4:60719152-60719171 MS.gene008629:CDS 40.0%
CGACATCAACATGTGTTAGT+CGG - chr3.4:60717286-60717305 None:intergenic 40.0%
CTTTGGCAATCTAGCATCTT+AGG - chr3.4:60718380-60718399 None:intergenic 40.0%
GAAACAAGGGATTCTCTTCA+GGG + chr3.4:60717870-60717889 MS.gene008629:intron 40.0%
GAAAGAGCTTGAAGAACATC+TGG - chr3.4:60716821-60716840 None:intergenic 40.0%
GAAGAAGGTGAATGTGATGA+AGG - chr3.4:60716651-60716670 None:intergenic 40.0%
GACATCAACATGTGTTAGTC+GGG - chr3.4:60717285-60717304 None:intergenic 40.0%
GATGTTAAAGCAAGATCCAC+CGG - chr3.4:60719948-60719967 None:intergenic 40.0%
GATGTTCTTCAAGCTCTTTC+TGG + chr3.4:60716821-60716840 MS.gene008629:CDS 40.0%
GATTGTACTGCACTATCTTC+CGG + chr3.4:60719926-60719945 MS.gene008629:CDS 40.0%
GGAGAAATGGTCACAAAGAA+AGG - chr3.4:60719362-60719381 None:intergenic 40.0%
GTCATAGGTGTCAACTGAAT+GGG + chr3.4:60717446-60717465 MS.gene008629:intron 40.0%
GTCTGACATCAACAAAACCA+TGG + chr3.4:60719301-60719320 MS.gene008629:CDS 40.0%
TAGAAGAAAGATGGGGATGA+AGG - chr3.4:60718159-60718178 None:intergenic 40.0%
TAGGCCTTTCATGAAGGTTT+CGG - chr3.4:60719612-60719631 None:intergenic 40.0%
TCAAAATACCACGTGGATCT+TGG + chr3.4:60719776-60719795 MS.gene008629:CDS 40.0%
TCATAGGTGTCAACTGAATG+GGG + chr3.4:60717447-60717466 MS.gene008629:intron 40.0%
TGGGGTTTATTCACCTTTGA+CGG + chr3.4:60719749-60719768 MS.gene008629:CDS 40.0%
TGTCATAGGTGTCAACTGAA+TGG + chr3.4:60717445-60717464 MS.gene008629:intron 40.0%
TGTGATGAAGGAAGAAACAG+TGG - chr3.4:60716639-60716658 None:intergenic 40.0%
! ACGATCATCTGATGGATCAA+CGG - chr3.4:60720071-60720090 None:intergenic 40.0%
! CGACATTTTGAGCATTCACA+AGG - chr3.4:60719817-60719836 None:intergenic 40.0%
! TGCAACTGCAAAAGCTTTAG+AGG + chr3.4:60719889-60719908 MS.gene008629:CDS 40.0%
! TTGGAGATTTCGAAAGGCAT+TGG + chr3.4:60719729-60719748 MS.gene008629:CDS 40.0%
!! TCTAACATTGGTGTCACTGT+TGG + chr3.4:60716845-60716864 MS.gene008629:CDS 40.0%
!! TGGAGATTTCGAAAGGCATT+GGG + chr3.4:60719730-60719749 MS.gene008629:CDS 40.0%
!!! GATCCACGTGGTATTTTGAT+TGG - chr3.4:60719775-60719794 None:intergenic 40.0%
!!! GTATTTTGATTGGCCGTCAA+AGG - chr3.4:60719765-60719784 None:intergenic 40.0%
AAACCTCCAAAGATGCCAAG+AGG - chr3.4:60720180-60720199 None:intergenic 45.0%
AACAACCCTCTTGGCATCTT+TGG + chr3.4:60720171-60720190 MS.gene008629:CDS 45.0%
AACCTCCAAAGATGCCAAGA+GGG - chr3.4:60720179-60720198 None:intergenic 45.0%
AACCTGCAACGATCATCTGA+TGG - chr3.4:60720079-60720098 None:intergenic 45.0%
AATAACCGGAGGTAGTGTTG+TGG + chr3.4:60718587-60718606 MS.gene008629:intron 45.0%
ACCTATGGCTTCAGAGATAG+AGG - chr3.4:60716696-60716715 None:intergenic 45.0%
ACGAAAGAAGCAATAACCGG+AGG + chr3.4:60718576-60718595 MS.gene008629:intron 45.0%
ACTACTTTCATGGGTGCTAG+TGG - chr3.4:60716746-60716765 None:intergenic 45.0%
AGAGTGGTATGTAGGAGAGT+TGG + chr3.4:60717498-60717517 MS.gene008629:intron 45.0%
AGCTGAAAGTTGCGACTTTG+GGG + chr3.4:60720027-60720046 MS.gene008629:CDS 45.0%
AGGATGCTACTAGGATTGCT+TGG + chr3.4:60718303-60718322 MS.gene008629:intron 45.0%
ATAGCAGCTCCGATTGTGAA+GGG - chr3.4:60719164-60719183 None:intergenic 45.0%
ATCCATCAGATGATCGTTGC+AGG + chr3.4:60720074-60720093 MS.gene008629:CDS 45.0%
ATGGGGATGAAGGGATAAGT+TGG - chr3.4:60718149-60718168 None:intergenic 45.0%
CATTTATAGCCTTCTCGACG+AGG + chr3.4:60719688-60719707 MS.gene008629:CDS 45.0%
CTACTTTCATGGGTGCTAGT+GGG - chr3.4:60716745-60716764 None:intergenic 45.0%
CTAGCCGAAACCTTCATGAA+AGG + chr3.4:60719605-60719624 MS.gene008629:CDS 45.0%
CTGTTAGCTAGGATGCTACT+AGG + chr3.4:60718294-60718313 MS.gene008629:intron 45.0%
CTTTCATGAAGGTTTCGGCT+AGG - chr3.4:60719607-60719626 None:intergenic 45.0%
GAACCACCTTCATAGCAACT+TGG + chr3.4:60719634-60719653 MS.gene008629:CDS 45.0%
GAATGCTTGCATTTCAGCGT+TGG + chr3.4:60717681-60717700 MS.gene008629:intron 45.0%
GCTCTTTCTGGGTCTAACAT+TGG + chr3.4:60716833-60716852 MS.gene008629:CDS 45.0%
GGAAACAAGGGATTCTCTTC+AGG + chr3.4:60717869-60717888 MS.gene008629:intron 45.0%
GGCTTCAGAGATAGAGGATA+TGG - chr3.4:60716690-60716709 None:intergenic 45.0%
GTCTGTTGTCTGACATGTGT+CGG - chr3.4:60717366-60717385 None:intergenic 45.0%
GTGTTCCCAAGTTGCTATGA+AGG - chr3.4:60719643-60719662 None:intergenic 45.0%
GTTCGTTAGGCCTTTCATGA+AGG - chr3.4:60719618-60719637 None:intergenic 45.0%
GTTTGCGAAGAGTGGTATGT+AGG + chr3.4:60717490-60717509 MS.gene008629:intron 45.0%
TCACAGGTGTCAACTGAATG+TGG + chr3.4:60717198-60717217 MS.gene008629:intron 45.0%
TCATGATTTCCACGCTGTCA+TGG - chr3.4:60718249-60718268 None:intergenic 45.0%
TGAAGCCATAGGTGTGAACT+GGG + chr3.4:60716703-60716722 MS.gene008629:CDS 45.0%
TGCTATGAAGGTGGTTCGTT+AGG - chr3.4:60719631-60719650 None:intergenic 45.0%
TGTATGTTTCGTGTGGTGGT+CGG - chr3.4:60719670-60719689 None:intergenic 45.0%
TTCCCAAGTTGCTATGAAGG+TGG - chr3.4:60719640-60719659 None:intergenic 45.0%
TTTCTAAGTGGAGTTGGGTG+AGG + chr3.4:60718689-60718708 MS.gene008629:intron 45.0%
! ACAGGGTGTGTAGTTTTCAG+TGG + chr3.4:60720134-60720153 MS.gene008629:CDS 45.0%
! AGGGATTCTCTTCAGGGTTT+TGG + chr3.4:60717876-60717895 MS.gene008629:intron 45.0%
!! GGAGATTTCGAAAGGCATTG+GGG + chr3.4:60719731-60719750 MS.gene008629:CDS 45.0%
!! GTGTCGGTTTCCAACACAAT+TGG - chr3.4:60717350-60717369 None:intergenic 45.0%
!! TCCTCTATCTCTGAAGCCAT+AGG + chr3.4:60716692-60716711 MS.gene008629:CDS 45.0%
AAATGTGAGGAGCTCGACCA+TGG - chr3.4:60719321-60719340 None:intergenic 50.0%
AACCCTCTTGGCATCTTTGG+AGG + chr3.4:60720174-60720193 MS.gene008629:CDS 50.0%
ACCTTCGTGAAGGGGTAAGA+TGG + chr3.4:60717819-60717838 MS.gene008629:intron 50.0%
CATAGCAGCTCCGATTGTGA+AGG - chr3.4:60719165-60719184 None:intergenic 50.0%
CGGCCAATCAAAATACCACG+TGG + chr3.4:60719769-60719788 MS.gene008629:CDS 50.0%
CGTGCTTGTCGAGAAATGTG+AGG - chr3.4:60719334-60719353 None:intergenic 50.0%
CTCTTCCACAACACTACCTC+CGG - chr3.4:60718595-60718614 None:intergenic 50.0%
CTGAAAGTTGCGACTTTGGG+GGG + chr3.4:60720029-60720048 MS.gene008629:CDS 50.0%
CTGAAGCCATAGGTGTGAAC+TGG + chr3.4:60716702-60716721 MS.gene008629:CDS 50.0%
GAAGCCATAGGTGTGAACTG+GGG + chr3.4:60716704-60716723 MS.gene008629:CDS 50.0%
GCTGAAAGTTGCGACTTTGG+GGG + chr3.4:60720028-60720047 MS.gene008629:CDS 50.0%
TCACAAGGCTCTTTGAACCC+TGG - chr3.4:60719802-60719821 None:intergenic 50.0%
TCCTTTGATCCTCGTCGAGA+AGG - chr3.4:60719700-60719719 None:intergenic 50.0%
TGTACTGCACTATCTTCCGG+TGG + chr3.4:60719929-60719948 MS.gene008629:CDS 50.0%
! CTTGCATTTCAGCGTTGGAG+CGG + chr3.4:60717686-60717705 MS.gene008629:intron 50.0%
AGGTGTGAACTGGGGAACCA+TGG + chr3.4:60716712-60716731 MS.gene008629:CDS 55.0%
ATACCACGTGGATCTTGGCC+AGG + chr3.4:60719781-60719800 MS.gene008629:CDS 55.0%
CAGCTCCGATTGTGAAGGGA+TGG - chr3.4:60719160-60719179 None:intergenic 55.0%
GAAGGTTTCGGCTAGGTACG+AGG - chr3.4:60719600-60719619 None:intergenic 55.0%
GCCATCTTACCCCTTCACGA+AGG - chr3.4:60717823-60717842 None:intergenic 55.0%
GCCTTCTCGACGAGGATCAA+AGG + chr3.4:60719696-60719715 MS.gene008629:CDS 55.0%
GGTTCCCCAGTTCACACCTA+TGG - chr3.4:60716711-60716730 None:intergenic 55.0%
GTCAGACCTGAGATGCGCTT+CGG - chr3.4:60719288-60719307 None:intergenic 55.0%
TACCACGTGGATCTTGGCCA+GGG + chr3.4:60719782-60719801 MS.gene008629:CDS 55.0%
! AAGATTGGTTGGCCGACCGA+TGG + chr3.4:60719560-60719579 MS.gene008629:CDS 55.0%
! CTTGAGTTGCCATGACAGCG+TGG + chr3.4:60718237-60718256 MS.gene008629:intron 55.0%
! GGTTGCGTTGGCAGATCCAT+CGG - chr3.4:60719579-60719598 None:intergenic 55.0%
! TGCTAGTGGGTGTGAAGCCA+TGG - chr3.4:60716732-60716751 None:intergenic 55.0%
AACCCTGGCCAAGATCCACG+TGG - chr3.4:60719787-60719806 None:intergenic 60.0%
AGACCTGAGATGCGCTTCGG+TGG - chr3.4:60719285-60719304 None:intergenic 60.0%
ATGCGCTTCGGTGGACGAGT+TGG - chr3.4:60719276-60719295 None:intergenic 60.0%
GGCTAGGTACGAGGTTGCGT+TGG - chr3.4:60719591-60719610 None:intergenic 60.0%
GTTTCGTGTGGTGGTCGGTG+TGG - chr3.4:60719665-60719684 None:intergenic 60.0%
! AGTTGCGACTTTGGGGGGCT+TGG + chr3.4:60720034-60720053 MS.gene008629:CDS 60.0%
! GCGTTGGCAGATCCATCGGT+CGG - chr3.4:60719575-60719594 None:intergenic 60.0%
CGTCCACCGAAGCGCATCTC+AGG + chr3.4:60719279-60719298 MS.gene008629:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 60716632 60720201 60716632 ID=MS.gene008629
chr3.4 mRNA 60716632 60720201 60716632 ID=MS.gene008629.t1;Parent=MS.gene008629
chr3.4 exon 60716632 60716972 60716632 ID=MS.gene008629.t1.exon1;Parent=MS.gene008629.t1
chr3.4 CDS 60716632 60716972 60716632 ID=cds.MS.gene008629.t1;Parent=MS.gene008629.t1
chr3.4 exon 60719106 60720201 60719106 ID=MS.gene008629.t1.exon2;Parent=MS.gene008629.t1
chr3.4 CDS 60719106 60720201 60719106 ID=cds.MS.gene008629.t1;Parent=MS.gene008629.t1
Gene Sequence

>MS.gene008629

ATGTCCACTGTTTCTTCCTTCATCACATTCACCTTCTTCTTCTTCACCATTTTCACCATATCCTCTATCTCTGAAGCCATAGGTGTGAACTGGGGAACCATGGCTTCACACCCACTAGCACCCATGAAAGTAGTGAAGCTTTTGAAGTCCAACAACATCAACAAAGTGAAACTGTTTGATGCAAAACCAGATGTTCTTCAAGCTCTTTCTGGGTCTAACATTGGTGTCACTGTTGGTATTCCTAATGTTTTGCTCAAAAGCTTGAATTCTTCTAGAAAAGTTGCTGATACTTGGGTTCATGATAATGTTACTCGCTATGTTTCTAATGGTGCTAAAATTGAGTATGTCATTTTTCTTTCTTCCTCTTTTAATTGTATATTTTCTCATTTTATATTATTTATTTCATTTTTTATTTAATATACTGTTGGTTATTTTTTTATTTATATTTTTTTTTCATATTTGTTGTTAACTTGTTAGGTTCTTTGGTAATAACGTAAAGACTTAGTAAGTGAAACAAGAATTAGTCACTGAATGAGGAAGTTTTATAGATTATAGAACAAATAATGTCACAGGTGTCAACTGAATGTGGTTTGAGTACTTGATCTCTTGATGCTACAAAAACCTATTAAACACGGACACATACACGTACACCCGACTAACACATGTTGATGTCGTCAACAATAATAATTTAGGAAAATGAATTAATAAAATGTAACCAATTGTGTTGGAAACCGACACATGTCAGACAACAGACACATTTTTGATTAGAAGTGTCGTGCTAGATGCGAAAACCCATAATTATAAAACAAACAATGTCATAGGTGTCAACTGAATGGGGTTTGTGTTATTAATAGTTAGGTTTGCGAAGAGTGGTATGTAGGAGAGTTGGAATGTGTCAAAGATAAAATGTAGGTACTGTGATAACTAAGTGGAAAAAGAACTAAACTTAGCTAAGCCAATGTTCATTGCAAATGAAAGCAGTGAATCAAATGCAGTAGTTATGATTCATGGCATGAAACAAATGTAAGAGGACTGCAATTGTTAGAATAGAATGCTTGCATTTCAGCGTTGGAGCGGCTCCACGATGTAAAATCTATAGATGGAATGTGATTTTAAAATAAATCAGTTTTTCTACCTTGTATGAGCTGAGAAAATTGGAAAATCAAATCAGTTATATTGAATTAATCACCTTCGTGAAGGGGTAAGATGGCACACATATTATGATGGTAAATTTTCTGGAAACAAGGGATTCTCTTCAGGGTTTTGGTCTCTTCTAGCCTGTAAAATAATTACATTATTTTAAACACAATATTCTTTCAAATGCAATAACATAAGAAACTTGATCAAAAAATAGTAGCAGAAGCAGAAAATTATAAACTTAATAGTGTAAAAGTTCTGTAGAAGTTTAATCTCATAAGGTAGAGAAGTTTATATTGCTTTAATATCGAGAATACCCCTCATTTTTGACACCTAACAGTAAGAATTATTTATGGATTTTGCGATGACTTAAAACACCAACTTATCCCTTCATCCCCATCTTTCTTCTATAGAGAAAAAGTTTATCCGAAATATAAAAATAAGATCCAAAGTGATTACAATTAGCAGCTTGAGTTGCCATGACAGCGTGGAAATCATGACTCATGATAAGAGTAAGACACATCACTGTTAGCTAGGATGCTACTAGGATTGCTTGGTGACTCTTCGTATTCTATGTTTCCATTCAAAATATGTCTAAACTCCCAAGTCCTAAGATGCTAGATTGCCAAAGAAATGATCAGCTGACATATTTAGGATCTGTTTGGTTTGAGAAAATAAGGAAGGAAATAATATGAAAATAAAGGCTAATCATGAAACAGGACAGTTATACAAAATCAAATCCCTATGATTAAGACTACAGAAGGTGTGTGTTGTTTTTCAATAACAAAGATGAATTGATGGCTGTTCACGAAAGAAGCAATAACCGGAGGTAGTGTTGTGGAAGAGTAGATTAGTGATACTGGTGTACATGATTTGTTGATACGATGACACTTTCAGCCATGTTGCAACTTCATTTTCACTTTCTAAGTGGAGTTGGGTGAGGTATTTCAACAATAAAATTTGTCAAAACTCTTTTCTTAGTCCATATAAGGAGATAATGACAGGAGGAGTTCCTAAGATATGTTCAACTAATTTTGCTTATAGTTTGAATACAAATATTTTAGATGGAAACTTTAACTTGGTCATTTTTCTTCTAAGAGTTGATTTTTGAACAGAATTAATGTTACTGTTTTCTCAGTGGTGTATGCTAGATCAGTTAAATTCACTAGCCATAGAGCATCAAATAAGTATGCTTTCTCAAAGGTTTAATCAGCTAAAATTTGAACAAGTAATTGAAACTTTTGAAAAAATTTACTCTTGGTTGAAAAAAAATGGATATGTATTTCTGAAGTATGACTCACTTTACATATATGGTTCTAAATTTAACTTATGTGCAGATATGTTGCTGTTGGTGATGAACCATTTCTTAAAAGTTACGGCGAACAATTCCATCCCTTCACAATCGGAGCTGCTATGAATATTCAAGCTGCTTTAAAGAAAGCAAAATTGGACAACAAAGTAAAAGTTGTAGTTCTATGCAGCTTTGATAGTTTTGAGTCAGGATCCAACTCGTCCACCGAAGCGCATCTCAGGTCTGACATCAACAAAACCATGGTCGAGCTCCTCACATTTCTCGACAAGCACGAATCACCTTTCTTTGTGACCATTTCTCCATTTCTAACTTTCCTTCAGACCAAAAACATTTCACTTGACTTTTCACTCTTCAAAGAAACTGCACACCCTCATAAACTTAGCCATAAAACATACAAAAATAGCTTCGATATGAGCTACGATACAGTGATCACTGTTTTATCAAAAGTAGGATTTCCTAATATGGAAATCGTTGTTTCAAAGATTGGTTGGCCGACCGATGGATCTGCCAACGCAACCTCGTACCTAGCCGAAACCTTCATGAAAGGCCTAACGAACCACCTTCATAGCAACTTGGGAACACCACACCGACCACCACACGAAACATACATTTATAGCCTTCTCGACGAGGATCAAAGGAGCATTATTGTTGGAGATTTCGAAAGGCATTGGGGTTTATTCACCTTTGACGGCCAATCAAAATACCACGTGGATCTTGGCCAGGGTTCAAAGAGCCTTGTGAATGCTCAAAATGTCGAGTATCTTTCTTCAAAGTGGTGTGTTGTGAACAACAACAAAGACTTGTCTAATGCAACTGCAAAAGCTTTAGAGGCTTGCGCAAATGCTGATTGTACTGCACTATCTTCCGGTGGATCTTGCTTTAACATCACATGGCCAAGTAATATATCATATGCTTTTAATAGTTATTATCAGGAGCATGATCAGAAAGCTGAAAGTTGCGACTTTGGGGGGCTTGGCTTAATCACAACCGTTGATCCATCAGATGATCGTTGCAGGTTTCCGATTGAGATTCACACATCTAATGCAGAACTTTACAGGGTGTGTAGTTTTCAGTGGTTGATTTTACTAGTAACAACCCTCTTGGCATCTTTGGAGGTTTGA
Protein sequence

>MS.gene008629.t1

MSTVSSFITFTFFFFTIFTISSISEAIGVNWGTMASHPLAPMKVVKLLKSNNINKVKLFDAKPDVLQALSGSNIGVTVGIPNVLLKSLNSSRKVADTWVHDNVTRYVSNGAKIEYVAVGDEPFLKSYGEQFHPFTIGAAMNIQAALKKAKLDNKVKVVVLCSFDSFESGSNSSTEAHLRSDINKTMVELLTFLDKHESPFFVTISPFLTFLQTKNISLDFSLFKETAHPHKLSHKTYKNSFDMSYDTVITVLSKVGFPNMEIVVSKIGWPTDGSANATSYLAETFMKGLTNHLHSNLGTPHRPPHETYIYSLLDEDQRSIIVGDFERHWGLFTFDGQSKYHVDLGQGSKSLVNAQNVEYLSSKWCVVNNNKDLSNATAKALEACANADCTALSSGGSCFNITWPSNISYAFNSYYQEHDQKAESCDFGGLGLITTVDPSDDRCRFPIEIHTSNAELYRVCSFQWLILLVTTLLASLEV