AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene47574

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47574.t1 MTR_4g088155 96.537 462 16 0 1 462 1 462 0.0 926
MS.gene47574.t1 MTR_7g053500 56.818 440 186 2 24 462 27 463 0.0 557
MS.gene47574.t1 MTR_8g012400 55.076 463 198 4 4 462 7 463 0.0 547
MS.gene47574.t1 MTR_7g053500 57.584 389 161 2 75 462 1 386 1.42e-176 500
MS.gene47574.t1 MTR_8g012400 55.784 389 165 3 75 462 1 383 3.34e-167 476
MS.gene47574.t1 MTR_1g056640 38.095 462 256 6 22 459 37 492 5.47e-114 344
MS.gene47574.t1 MTR_5g085580 38.053 452 259 6 22 459 24 468 1.40e-109 332
MS.gene47574.t1 MTR_4g069940 36.522 460 278 7 7 459 9 461 2.74e-103 316
MS.gene47574.t1 MTR_3g065460 36.774 465 263 8 4 458 3 446 4.63e-100 308
MS.gene47574.t1 MTR_6g088885 35.792 461 279 8 3 457 5 454 9.18e-99 305
MS.gene47574.t1 MTR_3g116510 35.088 456 286 6 3 457 8 454 1.99e-97 301
MS.gene47574.t1 MTR_5g078200 37.336 458 264 8 7 458 5 445 1.86e-96 303
MS.gene47574.t1 MTR_1g031900 36.147 462 273 9 1 457 1 445 8.36e-94 293
MS.gene47574.t1 MTR_1g031900 36.147 462 273 9 1 457 1 445 1.50e-93 292
MS.gene47574.t1 MTR_5g078200 37.529 429 247 7 34 458 1 412 8.81e-93 293
MS.gene47574.t1 MTR_8g085720 34.047 467 274 7 3 458 6 449 1.17e-92 290
MS.gene47574.t1 MTR_4g132280 36.486 444 253 7 23 458 45 467 4.95e-91 286
MS.gene47574.t1 MTR_1g022160 33.477 463 278 9 18 457 19 474 1.46e-88 278
MS.gene47574.t1 MTR_1g022160 33.477 463 278 9 18 457 19 474 2.96e-88 278
MS.gene47574.t1 MTR_3g083580 33.478 460 277 9 5 457 9 446 3.69e-88 278
MS.gene47574.t1 MTR_8g102340 34.773 440 241 8 19 457 24 418 9.76e-87 274
MS.gene47574.t1 MTR_1g022160 32.906 468 283 10 18 462 19 478 3.98e-85 270
MS.gene47574.t1 MTR_7g026340 34.163 442 272 11 21 457 21 448 5.01e-83 263
MS.gene47574.t1 MTR_4g084480 39.697 330 191 5 21 349 30 352 1.51e-81 257
MS.gene47574.t1 MTR_4g084480 39.697 330 191 5 21 349 30 352 2.32e-81 257
MS.gene47574.t1 MTR_4g084480 39.193 347 201 6 4 349 8 345 2.91e-81 256
MS.gene47574.t1 MTR_4g083500 33.624 458 279 8 17 458 38 486 8.62e-81 259
MS.gene47574.t1 MTR_3g080410 38.602 329 195 3 21 348 24 346 1.14e-80 254
MS.gene47574.t1 MTR_4g010200 36.494 348 208 5 24 370 34 369 2.00e-80 254
MS.gene47574.t1 MTR_1g077990 40.244 328 187 5 22 348 30 349 1.19e-78 250
MS.gene47574.t1 MTR_4g052970 38.226 327 194 5 24 349 30 349 2.01e-78 249
MS.gene47574.t1 MTR_2g019345 37.931 348 205 5 4 347 1 341 9.09e-77 245
MS.gene47574.t1 MTR_2g086530 37.318 343 195 6 22 358 7 335 4.44e-76 243
MS.gene47574.t1 MTR_2g086530 37.318 343 195 6 22 358 28 356 6.23e-76 243
MS.gene47574.t1 MTR_4g112025 32.762 467 287 17 4 461 12 460 6.32e-74 241
MS.gene47574.t1 MTR_1g053320 34.034 476 271 19 3 461 11 460 6.42e-74 241
MS.gene47574.t1 MTR_1g007810 37.941 340 197 7 8 344 1 329 7.19e-72 231
MS.gene47574.t1 MTR_1g010120 33.477 463 280 16 7 461 10 452 3.80e-70 230
MS.gene47574.t1 MTR_3g031770 33.703 451 265 17 17 458 21 446 4.17e-69 228
MS.gene47574.t1 MTR_1g022160 35.714 336 208 5 18 351 19 348 1.68e-68 224
MS.gene47574.t1 MTR_2g027560 34.521 449 262 17 19 457 24 450 1.07e-67 224
MS.gene47574.t1 MTR_3g095050 34.043 470 276 17 4 458 6 456 2.06e-67 223
MS.gene47574.t1 MTR_8g092070 33.632 446 265 19 23 458 34 458 2.93e-67 226
MS.gene47574.t1 MTR_3g116510 35.276 326 204 5 133 457 1 320 4.35e-67 218
MS.gene47574.t1 MTR_7g081350 35.673 342 201 7 4 345 13 335 6.01e-67 218
MS.gene47574.t1 MTR_4g124440 33.049 469 277 21 6 461 11 455 7.48e-67 222
MS.gene47574.t1 MTR_4g114850 34.251 327 208 4 24 349 27 347 1.85e-66 218
MS.gene47574.t1 MTR_3g108600 33.405 464 256 17 5 461 22 439 2.36e-66 220
MS.gene47574.t1 MTR_4g114850 34.251 327 208 4 24 349 27 347 2.89e-66 217
MS.gene47574.t1 MTR_4g114850 34.251 327 208 4 24 349 27 347 6.93e-66 216
MS.gene47574.t1 MTR_4g076255 36.196 326 187 8 23 347 25 330 4.86e-65 213
MS.gene47574.t1 MTR_2g034440 37.805 328 182 11 12 336 15 323 5.11e-65 213
MS.gene47574.t1 MTR_2g034470 36.599 347 198 11 12 355 23 350 1.80e-64 212
MS.gene47574.t1 MTR_4g076490 34.848 330 195 7 21 349 95 405 2.62e-64 213
MS.gene47574.t1 MTR_1g052430 36.499 337 194 8 13 345 22 342 1.40e-62 209
MS.gene47574.t1 MTR_4g076430 34.870 347 202 10 4 347 3 328 1.02e-61 204
MS.gene47574.t1 MTR_4g076500 38.415 328 183 8 23 347 78 389 1.70e-61 205
MS.gene47574.t1 MTR_2g034470 36.061 330 193 10 29 355 1 315 3.86e-61 202
MS.gene47574.t1 MTR_4g076440 33.815 346 207 9 4 347 3 328 8.77e-61 201
MS.gene47574.t1 MTR_4g076570 36.615 325 192 6 22 345 21 332 3.55e-60 201
MS.gene47574.t1 MTR_7g081370 32.362 343 203 7 5 345 8 323 9.01e-58 194
MS.gene47574.t1 MTR_4g076470 32.817 323 196 8 23 344 23 325 4.68e-57 192
MS.gene47574.t1 MTR_2g034480 33.711 353 210 11 4 352 12 344 7.03e-57 192
MS.gene47574.t1 MTR_7g085390 30.942 446 276 18 23 457 26 450 2.00e-56 194
MS.gene47574.t1 MTR_6g032820 31.336 434 261 19 23 441 26 437 2.41e-53 185
MS.gene47574.t1 MTR_4g134280 35.933 359 195 19 110 457 2 336 1.04e-48 171
MS.gene47574.t1 MTR_8g063490 65.217 115 27 2 294 406 1 104 2.36e-45 154
MS.gene47574.t1 MTR_5g081720 33.439 314 184 8 39 344 53 349 1.75e-44 160
MS.gene47574.t1 MTR_8g471000 76.471 85 20 0 128 212 1 85 2.50e-39 137
MS.gene47574.t1 MTR_4g133730 41.176 85 50 0 373 457 28 112 4.22e-19 83.2
MS.gene47574.t1 MTR_5g097420 40.230 87 52 0 371 457 28 114 8.04e-19 82.4
MS.gene47574.t1 MTR_4g133735 38.824 85 52 0 373 457 28 112 3.96e-17 77.8
MS.gene47574.t1 MTR_4g036680 35.922 103 59 2 243 345 80 175 2.33e-15 75.5
MS.gene47574.t1 MTR_4g036775 35.922 103 59 2 243 345 80 175 2.85e-15 75.1
MS.gene47574.t1 MTR_8g089400 35.238 105 61 3 359 459 22 123 1.12e-14 72.0
MS.gene47574.t1 MTR_1g116870 34.737 95 61 1 363 457 266 359 1.77e-14 75.1
MS.gene47574.t1 MTR_2g049670 44.706 85 45 2 373 457 158 240 2.42e-14 74.3
MS.gene47574.t1 MTR_5g091650 39.560 91 54 1 370 460 105 194 3.52e-14 71.6
MS.gene47574.t1 MTR_8g089400 35.238 105 61 3 359 459 42 143 3.64e-14 70.9
MS.gene47574.t1 MTR_5g015720 36.275 102 64 1 357 457 313 414 7.55e-14 73.6
MS.gene47574.t1 MTR_5g091650 39.560 91 54 1 369 459 104 193 7.74e-14 72.0
MS.gene47574.t1 MTR_8g085440 36.667 90 56 1 369 458 28 116 9.40e-14 68.2
MS.gene47574.t1 MTR_7g111600 38.372 86 52 1 373 458 160 244 6.01e-13 69.7
MS.gene47574.t1 MTR_7g111600 38.372 86 52 1 373 458 160 244 7.67e-13 68.9
MS.gene47574.t1 MTR_3g082080 36.047 86 54 1 367 452 151 235 1.04e-12 68.2
MS.gene47574.t1 MTR_7g111600 38.372 86 52 1 373 458 160 244 1.07e-12 69.3
MS.gene47574.t1 MTR_3g048280 38.384 99 58 3 358 454 95 192 1.39e-12 67.0
MS.gene47574.t1 MTR_4g079530 36.264 91 58 0 369 459 33 123 1.70e-12 64.7
MS.gene47574.t1 MTR_4g079530 36.264 91 58 0 369 459 33 123 2.15e-12 65.9
MS.gene47574.t1 MTR_8g088120 37.179 78 48 1 382 459 57 133 2.83e-11 63.5
MS.gene47574.t1 MTR_8g085390 39.080 87 52 1 373 459 30 115 3.26e-11 62.8
MS.gene47574.t1 MTR_1g084820 40.476 84 46 3 369 451 416 496 3.27e-11 65.9
MS.gene47574.t1 MTR_1g084820 40.476 84 46 3 369 451 416 496 3.45e-11 65.9
MS.gene47574.t1 MTR_8g085420 35.227 88 56 1 369 456 28 114 9.58e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47574.t1 AT3G23770 63.801 442 160 0 21 462 35 476 0.0 611
MS.gene47574.t1 AT4G14080 59.459 481 173 2 1 462 1 478 0.0 601
MS.gene47574.t1 AT3G07320 55.310 452 196 3 12 462 14 460 0.0 536
MS.gene47574.t1 AT4G26830 37.284 464 273 9 1 459 3 453 5.28e-112 338
MS.gene47574.t1 AT2G05790 38.852 453 256 7 22 459 24 470 9.50e-111 335
MS.gene47574.t1 AT5G55180 38.149 443 257 6 23 459 27 458 1.12e-110 335
MS.gene47574.t1 AT4G26830 37.415 441 260 7 23 459 29 457 1.17e-110 335
MS.gene47574.t1 AT5G55180 38.303 436 252 6 23 452 27 451 5.01e-108 328
MS.gene47574.t1 AT5G56590 35.845 438 268 7 23 457 26 453 2.90e-103 317
MS.gene47574.t1 AT2G01630 38.600 443 251 7 20 458 20 445 8.47e-102 313
MS.gene47574.t1 AT2G01630 38.303 436 248 7 20 451 20 438 2.23e-98 302
MS.gene47574.t1 AT4G29360 36.920 474 268 7 6 457 11 475 3.91e-98 305
MS.gene47574.t1 AT4G29360 37.037 459 259 6 21 457 35 485 1.00e-97 304
MS.gene47574.t1 AT1G66250 37.528 445 256 8 17 457 26 452 1.90e-97 302
MS.gene47574.t1 AT5G24318 36.087 460 274 11 4 457 10 455 1.70e-96 298
MS.gene47574.t1 AT5G24318 36.404 456 270 11 4 453 10 451 1.27e-95 296
MS.gene47574.t1 AT4G34480 35.242 454 279 7 6 458 9 448 1.09e-91 287
MS.gene47574.t1 AT1G11820 34.685 444 261 7 23 458 44 466 1.16e-90 285
MS.gene47574.t1 AT3G13560 35.763 439 260 6 23 457 26 446 2.22e-90 284
MS.gene47574.t1 AT3G13560 35.763 439 260 6 23 457 26 446 2.22e-90 284
MS.gene47574.t1 AT3G13560 35.763 439 260 6 23 457 26 446 2.22e-90 284
MS.gene47574.t1 AT3G13560 35.763 439 260 6 23 457 26 446 2.22e-90 284
MS.gene47574.t1 AT3G13560 35.763 439 260 6 23 457 26 446 2.22e-90 284
MS.gene47574.t1 AT2G16230 34.452 447 273 8 17 458 19 450 3.90e-88 278
MS.gene47574.t1 AT1G11820 34.247 438 259 7 23 452 44 460 5.28e-88 277
MS.gene47574.t1 AT3G55430 35.520 442 261 13 21 457 27 449 2.10e-86 272
MS.gene47574.t1 AT2G01630 37.783 397 216 8 20 412 20 389 4.99e-81 256
MS.gene47574.t1 AT5G24318 39.481 347 197 7 4 347 10 346 1.91e-79 251
MS.gene47574.t1 AT4G18340 36.261 353 217 5 3 354 14 359 4.67e-78 248
MS.gene47574.t1 AT4G18340 36.261 353 217 5 3 354 14 359 1.19e-77 248
MS.gene47574.t1 AT5G42720 36.612 366 220 6 6 367 11 368 1.16e-76 246
MS.gene47574.t1 AT1G32860 37.830 341 206 4 6 345 12 347 1.17e-75 243
MS.gene47574.t1 AT2G27500 36.585 328 201 4 23 349 29 350 1.46e-75 241
MS.gene47574.t1 AT2G27500 36.585 328 201 4 23 349 29 350 5.64e-75 240
MS.gene47574.t1 AT2G39640 32.527 455 281 14 6 458 13 443 2.55e-74 243
MS.gene47574.t1 AT3G24330 32.618 466 284 16 6 461 23 468 7.24e-74 241
MS.gene47574.t1 AT1G30080 37.423 326 196 5 21 345 34 352 2.10e-73 236
MS.gene47574.t1 AT1G30080 37.423 326 196 5 21 345 34 352 6.51e-73 236
MS.gene47574.t1 AT5G42100 37.537 341 204 6 10 347 14 348 2.63e-71 232
MS.gene47574.t1 AT3G61810 37.952 332 186 4 23 348 55 372 3.86e-71 230
MS.gene47574.t1 AT5G42100 37.537 341 204 6 10 347 14 348 4.93e-71 231
MS.gene47574.t1 AT4G34480 35.429 350 219 4 7 355 10 353 8.75e-70 226
MS.gene47574.t1 AT3G15800 35.736 333 206 6 24 354 44 370 1.16e-69 227
MS.gene47574.t1 AT4G31140 33.617 470 277 19 3 461 7 452 1.27e-69 229
MS.gene47574.t1 AT3G57270 39.634 328 180 8 23 347 27 339 1.75e-69 224
MS.gene47574.t1 AT1G11820 35.650 331 202 5 23 349 44 367 1.35e-66 218
MS.gene47574.t1 AT4G34480 36.103 349 208 7 111 458 3 337 1.92e-66 218
MS.gene47574.t1 AT5G24318 35.890 326 195 7 136 457 1 316 2.11e-65 213
MS.gene47574.t1 AT5G58090 33.925 451 267 19 21 461 20 449 5.56e-65 217
MS.gene47574.t1 AT5G18220 32.484 471 281 19 5 461 12 459 1.96e-64 216
MS.gene47574.t1 AT4G16260 36.723 354 201 11 4 354 8 341 4.22e-64 211
MS.gene47574.t1 AT2G26600 32.394 355 233 4 24 377 35 383 8.26e-64 211
MS.gene47574.t1 AT4G17180 33.621 464 268 18 4 457 7 440 1.25e-62 211
MS.gene47574.t1 AT3G57260 36.337 344 201 7 4 347 13 338 2.60e-62 206
MS.gene47574.t1 AT2G19440 33.259 451 268 18 23 462 21 449 3.07e-62 210
MS.gene47574.t1 AT2G19440 33.259 451 268 18 23 462 21 449 3.07e-62 210
MS.gene47574.t1 AT3G57260 36.337 344 201 7 4 347 29 354 3.11e-62 206
MS.gene47574.t1 AT5G20340 35.522 335 200 9 13 345 33 353 1.16e-61 204
MS.gene47574.t1 AT5G20870 32.347 473 280 20 4 458 10 460 9.20e-61 206
MS.gene47574.t1 AT3G57240 37.386 329 187 9 19 347 31 340 1.84e-60 201
MS.gene47574.t1 AT1G64760 32.450 453 269 19 23 462 25 453 4.37e-60 204
MS.gene47574.t1 AT1G64760 32.450 453 269 19 23 462 25 453 4.37e-60 204
MS.gene47574.t1 AT4G16260 37.386 329 186 10 29 354 1 312 9.89e-60 198
MS.gene47574.t1 AT1G33220 34.294 347 196 10 7 345 12 334 1.34e-59 199
MS.gene47574.t1 AT5G20390 34.018 341 209 10 7 345 17 343 5.50e-59 197
MS.gene47574.t1 AT5G20330 34.870 347 204 10 7 345 12 344 1.01e-58 197
MS.gene47574.t1 AT5G58480 30.856 444 273 14 23 457 26 444 1.02e-58 200
MS.gene47574.t1 AT3G46570 34.783 345 209 10 7 347 12 344 2.03e-58 196
MS.gene47574.t1 AT3G04010 31.210 471 286 19 3 461 17 461 5.08e-58 199
MS.gene47574.t1 AT2G27500 35.018 277 173 4 74 349 2 272 5.30e-58 194
MS.gene47574.t1 AT1G77780 32.565 347 212 10 6 345 1 332 4.27e-53 182
MS.gene47574.t1 AT5G64790 29.526 464 296 18 9 462 14 456 7.41e-52 182
MS.gene47574.t1 AT1G77790 31.195 343 224 6 4 345 10 341 2.53e-51 177
MS.gene47574.t1 AT2G26600 32.203 295 193 4 84 377 1 289 5.07e-50 172
MS.gene47574.t1 AT3G55780 32.335 334 208 9 23 348 31 354 1.26e-49 174
MS.gene47574.t1 AT5G20560 31.307 329 212 8 7 334 18 333 1.49e-49 172
MS.gene47574.t1 AT4G05430 38.000 100 61 1 359 457 8 107 1.13e-17 80.1
MS.gene47574.t1 AT4G05430 38.947 95 57 1 359 452 33 127 1.42e-17 79.3
MS.gene47574.t1 AT4G05430 38.000 100 61 1 359 457 33 132 2.15e-17 80.1
MS.gene47574.t1 AT5G67460 38.679 106 58 2 356 457 275 377 4.64e-17 83.2
MS.gene47574.t1 AT2G04910 37.500 88 55 0 373 460 15 102 1.11e-16 75.9
MS.gene47574.t1 AT5G35740 38.372 86 52 1 373 457 30 115 7.63e-16 73.9
MS.gene47574.t1 AT2G30933 39.623 106 60 3 358 460 64 168 1.06e-15 76.6
MS.gene47574.t1 AT5G67460 38.614 101 55 2 356 452 275 372 1.93e-15 78.2
MS.gene47574.t1 AT1G66855 36.364 88 56 0 370 457 23 110 9.59e-15 70.9
MS.gene47574.t1 AT1G66870 32.955 88 59 0 370 457 50 137 2.58e-14 70.5
MS.gene47574.t1 AT1G29380 41.379 87 50 1 373 459 148 233 4.22e-14 72.8
MS.gene47574.t1 AT1G29380 41.176 85 49 1 373 457 148 231 4.57e-14 73.6
MS.gene47574.t1 AT1G66870 32.955 88 59 0 370 457 23 110 4.60e-14 68.9
MS.gene47574.t1 AT5G63250 38.202 89 53 1 373 459 42 130 5.89e-14 69.3
MS.gene47574.t1 AT1G09460 39.326 89 51 2 369 457 133 218 9.26e-14 72.8
MS.gene47574.t1 AT2G04910 36.250 80 51 0 373 452 15 94 4.17e-13 66.6
MS.gene47574.t1 AT3G58100 32.584 89 60 0 371 459 39 127 1.72e-12 66.2
MS.gene47574.t1 AT2G30933 38.144 97 56 3 358 451 64 159 1.94e-12 65.5
MS.gene47574.t1 AT2G30933 38.144 97 56 3 358 451 64 159 1.94e-12 65.5
MS.gene47574.t1 AT1G29380 40.244 82 48 1 373 454 148 228 3.55e-12 66.2
MS.gene47574.t1 AT1G29380 40.244 82 48 1 373 454 148 228 3.55e-12 66.2
MS.gene47574.t1 AT3G58100 32.584 89 60 0 371 459 115 203 3.87e-12 66.6
MS.gene47574.t1 AT1G79480 32.941 85 56 1 373 457 310 393 4.48e-12 68.2
MS.gene47574.t1 AT1G79480 32.941 85 56 1 373 457 311 394 4.83e-12 67.8
MS.gene47574.t1 AT1G18650 34.483 87 56 1 373 459 21 106 5.37e-12 63.5
MS.gene47574.t1 AT1G66852 33.333 90 55 2 370 457 23 109 1.34e-11 61.6
MS.gene47574.t1 AT5G63240 33.333 102 61 3 360 459 32 128 2.58e-11 61.6
MS.gene47574.t1 AT4G13600 35.556 90 57 1 372 461 60 148 4.36e-11 63.2
MS.gene47574.t1 AT5G61130 33.721 86 56 1 373 458 21 105 8.13e-11 62.0

Find 132 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTGTGACCATGGGAAATA+TGG 0.172066 4.3:-25314285 None:intergenic
CATCGTTGTTAGTAATTCTA+TGG 0.198229 4.3:-25313800 None:intergenic
GTTAAGTCTAGTTCATAAAT+TGG 0.210610 4.3:-25314702 None:intergenic
CTGTTCCAGGTCCTGACTTT+TGG 0.217083 4.3:-25314644 None:intergenic
AAGTAGCGGAACAGGGTATT+TGG 0.222064 4.3:-25313991 None:intergenic
ATTGGGACGCCAGGGCGTGC+TGG 0.242633 4.3:-25314588 None:intergenic
AACAGGGTATTTGGGTAGTA+TGG 0.258964 4.3:-25313982 None:intergenic
CAGCAATGCCTGAGATTTCA+TGG 0.261581 4.3:-25313915 None:intergenic
CTGTAGATTAAGCCACTATC+AGG 0.265102 4.3:-25314366 None:intergenic
TAGGAACTTACTAGGGTCTT+TGG 0.284953 4.3:-25315021 None:intergenic
TGAGCTTTGTGGTTGATAAA+AGG 0.285457 4.3:-25313870 None:intergenic
TTGGGACGCCAGGGCGTGCT+GGG 0.295213 4.3:-25314587 None:intergenic
TTGTATGGCACATTGTTCTT+TGG 0.295488 4.3:-25314765 None:intergenic
CCCCAGTGTCGTTCTGTTCC+AGG 0.305239 4.3:-25314657 None:intergenic
TGCCTGAGATTTCATGGTTT+GGG 0.310176 4.3:-25313909 None:intergenic
AACATCAGAGGCATCAAAAT+TGG 0.316037 4.3:+25314120 MS.gene47574:CDS
CTGTTCCGCTACTTACTTGT+AGG 0.323753 4.3:+25314000 MS.gene47574:CDS
GTTCACACCAATATTGCTAT+TGG 0.324826 4.3:-25313737 None:intergenic
TTAAGTCTAGTTCATAAATT+GGG 0.327852 4.3:-25314701 None:intergenic
CACCATACCTTGCCTTTGTA+TGG 0.329187 4.3:-25314780 None:intergenic
GTTTGGAGAAATGGATTATC+TGG 0.335991 4.3:+25313593 None:intergenic
CCCATTCATCTGCTATGCTT+TGG 0.343713 4.3:-25313942 None:intergenic
CTACAAGTAAGTAGCGGAAC+AGG 0.345027 4.3:-25313999 None:intergenic
CTAAGACATCCATGGCTAAT+GGG 0.352075 4.3:-25314146 None:intergenic
GCAAAAGCCAATAGCAATAT+TGG 0.354290 4.3:+25313730 MS.gene47574:CDS
TCTTCTATGTCACCGGAGTT+TGG 0.363338 4.3:-25314483 None:intergenic
ATGCCTGAGATTTCATGGTT+TGG 0.373081 4.3:-25313910 None:intergenic
AGCAATATTGGTGTGAACTA+TGG 0.382104 4.3:+25313742 MS.gene47574:CDS
AAAACGTAACTGGCATGAAC+AGG 0.382615 4.3:-25314927 None:intergenic
TCTAAGACATCCATGGCTAA+TGG 0.385476 4.3:-25314147 None:intergenic
GATAAAAGGCTTAGAATATC+CGG 0.387118 4.3:-25313856 None:intergenic
GAGAAGAGGAAGGTTGGTAT+TGG 0.399570 4.3:-25314606 None:intergenic
CAAGTTTCGACATAGCAAAA+AGG 0.407301 4.3:-25314419 None:intergenic
AGAAGAGGAAGGTTGGTATT+GGG 0.411195 4.3:-25314605 None:intergenic
AGGGCGTGCTGGGGTTCCGA+TGG 0.418653 4.3:-25314577 None:intergenic
TAGTCTAGGGGAATATTGTT+GGG 0.419210 4.3:-25314309 None:intergenic
TGGGCCCAGTTCAAAACTAA+TGG 0.423053 4.3:+25314960 MS.gene47574:CDS
AATTTATGAACTAGACTTAA+CGG 0.423395 4.3:+25314704 MS.gene47574:CDS
AGGAGCAGATGAAACAGAAT+TGG 0.425999 4.3:+25314815 MS.gene47574:CDS
ACCTGGAACAGAACGACACT+GGG 0.435501 4.3:+25314656 MS.gene47574:CDS
TTCATGGTTTGGGATCATAA+TGG 0.438321 4.3:-25313899 None:intergenic
GGAGTTTGGCCATCCTGTTT+CGG 0.439261 4.3:-25314469 None:intergenic
ATTTATGAACTAGACTTAAC+GGG 0.440218 4.3:+25314705 MS.gene47574:CDS
ACCTGTTTCTTCTATGTCAC+CGG 0.446920 4.3:-25314490 None:intergenic
GCATGAACAGGGATTGAAAC+TGG 0.447483 4.3:-25314915 None:intergenic
ACTTGTGATGCACTTTCACC+TGG 0.452950 4.3:+25314876 MS.gene47574:CDS
AAGGTTGGTATTGGGACGCC+AGG 0.460472 4.3:-25314597 None:intergenic
AGTAGCGGAACAGGGTATTT+GGG 0.463795 4.3:-25313990 None:intergenic
ACCAAAGCATAGCAGATGAA+TGG 0.464106 4.3:+25313941 MS.gene47574:CDS
CAGCTTGTAATATCCGAAAC+AGG 0.467943 4.3:+25314456 MS.gene47574:CDS
ACCACAAGAGACCCTGATAG+TGG 0.469191 4.3:+25314354 MS.gene47574:CDS
AATGGAAACTTTGAGCTTTG+TGG 0.471458 4.3:-25313881 None:intergenic
CTTTGGTTGTTTCTGTAGCA+AGG 0.471794 4.3:-25315004 None:intergenic
GATCGGAATGTTCCGCTTGA+CGG 0.477679 4.3:-25314183 None:intergenic
AAAGGAGGGAGTCAAGCATT+TGG 0.479957 4.3:-25314401 None:intergenic
CCAAAGCATAGCAGATGAAT+GGG 0.483302 4.3:+25313942 MS.gene47574:CDS
TTGTTCTTTGGAGCTTGTAA+TGG 0.483493 4.3:-25314753 None:intergenic
ATCCCAAACCATGAAATCTC+AGG 0.486232 4.3:+25313907 MS.gene47574:CDS
GGAGCCACTTGCTATTTCAA+TGG 0.489535 4.3:+25314981 MS.gene47574:CDS
GTCTCTAGAGAATTACGCTT+GGG 0.492062 4.3:-25315157 None:intergenic
ATAGTCTAGGGGAATATTGT+TGG 0.494497 4.3:-25314310 None:intergenic
TCCGGTGACATAGAAGAAAC+AGG 0.494666 4.3:+25314489 MS.gene47574:CDS
GGGCGTGCTGGGGTTCCGAT+GGG 0.498266 4.3:-25314576 None:intergenic
TAAAGTGAGAAGAGGAAGGT+TGG 0.499588 4.3:-25314612 None:intergenic
GGATATTACAAGCTGGATGT+TGG 0.499928 4.3:-25314448 None:intergenic
CGTGCTGGGGTTCCGATGGG+TGG 0.501818 4.3:-25314573 None:intergenic
TTGTTGGGGTCCTGTGACCA+TGG 0.503564 4.3:-25314294 None:intergenic
GACCTGGAACAGAACGACAC+TGG 0.510156 4.3:+25314655 MS.gene47574:CDS
ATCGGAACCCCAGCACGCCC+TGG 0.510521 4.3:+25314579 MS.gene47574:CDS
CACTGTCTCTTATGTCGGAT+CGG 0.511588 4.3:-25314200 None:intergenic
GTGTGAACTATGGCGTGCTT+GGG 0.520155 4.3:+25313752 MS.gene47574:CDS
GGTGTGAACTATGGCGTGCT+TGG 0.521584 4.3:+25313751 MS.gene47574:CDS
CTGTCTAAGAATTTGAGCAT+TGG 0.522756 4.3:-25314231 None:intergenic
CTCTGAAGAGAGCATAGTCT+AGG 0.524797 4.3:-25314323 None:intergenic
CCCATATTTCCCATGGTCAC+AGG 0.525831 4.3:+25314284 MS.gene47574:CDS
CAGAACAAGGTCACAAAATG+TGG 0.544995 4.3:+25314046 MS.gene47574:CDS
TACGACGAAAACCAAAAGTC+AGG 0.545569 4.3:+25314633 MS.gene47574:CDS
TGCCATACAAAGGCAAGGTA+TGG 0.546385 4.3:+25314778 MS.gene47574:CDS
CCTGTGACCATGGGAAATAT+GGG 0.546906 4.3:-25314284 None:intergenic
TCTGAAGAGAGCATAGTCTA+GGG 0.547953 4.3:-25314322 None:intergenic
ATAAAAGGCTTAGAATATCC+GGG 0.553913 4.3:-25313855 None:intergenic
GTTTGCTACATTGCAAGCAT+AGG 0.561983 4.3:-25314850 None:intergenic
ATGCATACCCATATTTCCCA+TGG 0.571377 4.3:+25314277 MS.gene47574:CDS
TGGGACGCCAGGGCGTGCTG+GGG 0.571585 4.3:-25314586 None:intergenic
CTGTTTCGGATATTACAAGC+TGG 0.574803 4.3:-25314455 None:intergenic
CTAGACTATGCTCTCTTCAG+AGG 0.575011 4.3:+25314324 MS.gene47574:CDS
CATCACACTGTCTCTTATGT+CGG 0.575279 4.3:-25314205 None:intergenic
TTACTAACAACGATGAAAGC+TGG 0.576835 4.3:+25313808 MS.gene47574:CDS
AGTCTCTAGAGAATTACGCT+TGG 0.577469 4.3:-25315158 None:intergenic
CGGAATGTTCCGCTTGACGG+TGG 0.579777 4.3:-25314180 None:intergenic
TGTTCCGCTTGACGGTGGGA+AGG 0.582463 4.3:-25314175 None:intergenic
CTAAGCTACAATTCAGAACA+AGG 0.590477 4.3:+25314033 MS.gene47574:CDS
AGTAATTCTATGGATCGATA+CGG 0.593771 4.3:-25313790 None:intergenic
ACAGGGTATTTGGGTAGTAT+GGG 0.595098 4.3:-25313981 None:intergenic
GGGTATTTGGGTAGTATGGG+AGG 0.595469 4.3:-25313978 None:intergenic
TGGGGCTTATTACACACAGA+TGG 0.597574 4.3:+25314675 MS.gene47574:CDS
TAAGTCTAGTTCATAAATTG+GGG 0.599115 4.3:-25314700 None:intergenic
AATTGGCACCCCATTAGCCA+TGG 0.599239 4.3:+25314137 MS.gene47574:CDS
TGTAGATTAAGCCACTATCA+GGG 0.604866 4.3:-25314365 None:intergenic
TAAGACATCCATGGCTAATG+GGG 0.605089 4.3:-25314145 None:intergenic
TCAACCTTCCCACCGTCAAG+CGG 0.605172 4.3:+25314171 MS.gene47574:CDS
GGAATGTTCCGCTTGACGGT+GGG 0.607933 4.3:-25314179 None:intergenic
GCCACTATCAGGGTCTCTTG+TGG 0.608331 4.3:-25314355 None:intergenic
TCTATGGATCGATACGGAGA+GGG 0.608408 4.3:-25313784 None:intergenic
AGGTTGGTATTGGGACGCCA+GGG 0.610142 4.3:-25314596 None:intergenic
TTGTAATATCCGAAACAGGA+TGG 0.610704 4.3:+25314460 MS.gene47574:CDS
GTATGGTGTGTGGCTGCAGA+AGG 0.611532 4.3:+25314795 MS.gene47574:CDS
TTTCGTCGTAAAGTGAGAAG+AGG 0.612513 4.3:-25314620 None:intergenic
GAAACAGGATGGCCAAACTC+CGG 0.614834 4.3:+25314471 MS.gene47574:CDS
GCATAGCAGATGAATGGGTG+AGG 0.616109 4.3:+25313947 MS.gene47574:CDS
CTCAATTCTCAGAACATCAG+AGG 0.619397 4.3:+25314108 MS.gene47574:CDS
GAAAACCAAAAGTCAGGACC+TGG 0.622204 4.3:+25314639 MS.gene47574:CDS
GGTTGATTCTAAGACATCCA+TGG 0.623476 4.3:-25314154 None:intergenic
AAGAACAATGTGCCATACAA+AGG 0.624147 4.3:+25314768 MS.gene47574:CDS
TGTTGGGGTCCTGTGACCAT+GGG 0.639282 4.3:-25314293 None:intergenic
CAATGTGCCATACAAAGGCA+AGG 0.639681 4.3:+25314773 MS.gene47574:CDS
GTCGTAAAGTGAGAAGAGGA+AGG 0.641080 4.3:-25314616 None:intergenic
AAGGCTTAGAATATCCGGGT+TGG 0.641156 4.3:-25313851 None:intergenic
AAACGTAACTGGCATGAACA+GGG 0.641773 4.3:-25314926 None:intergenic
TTCTATGGATCGATACGGAG+AGG 0.647784 4.3:-25313785 None:intergenic
AGTCTAGGGGAATATTGTTG+GGG 0.649812 4.3:-25314308 None:intergenic
GAAGCTCTACGACGCCAACC+CGG 0.650383 4.3:+25313837 MS.gene47574:CDS
TACAAGTAAGTAGCGGAACA+GGG 0.656576 4.3:-25313998 None:intergenic
AAGGCCATTGAAATAGCAAG+TGG 0.663975 4.3:-25314985 None:intergenic
CTGAAGAGAGCATAGTCTAG+GGG 0.665261 4.3:-25314321 None:intergenic
CATTTCCTACAAGTAAGTAG+CGG 0.676069 4.3:-25314005 None:intergenic
ATGACAGCAAAACCACCCAT+CGG 0.676239 4.3:+25314561 MS.gene47574:CDS
CGACATAAGAGACAGTGTGA+TGG 0.681532 4.3:+25314206 MS.gene47574:CDS
CAAAGGCAAGGTATGGTGTG+TGG 0.700494 4.3:+25314785 MS.gene47574:CDS
TGGCGTCGTAGAGCTTCACA+CGG 0.725730 4.3:-25313831 None:intergenic
CCTGGAACAGAACGACACTG+GGG 0.780167 4.3:+25314657 MS.gene47574:CDS
AGGTGCAAACATCTACAACG+CGG 0.808340 4.3:+25314509 MS.gene47574:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTATGAACTAGACTTAA+CGG + chr4.3:25314704-25314723 MS.gene47574:CDS 20.0%
!! ATCAATGAAGAAAAATGAAT+TGG - chr4.3:25314259-25314278 None:intergenic 20.0%
!!! TTAAGTCTAGTTCATAAATT+GGG - chr4.3:25314704-25314723 None:intergenic 20.0%
! AATGTAATTGTTTATGTGAC+AGG + chr4.3:25315116-25315135 MS.gene47574:intron 25.0%
! ATTTATGAACTAGACTTAAC+GGG + chr4.3:25314705-25314724 MS.gene47574:CDS 25.0%
! GTCACATAAACAATTACATT+AGG - chr4.3:25315116-25315135 None:intergenic 25.0%
! TATTGTAAAGAGAGTAAAGT+AGG - chr4.3:25315043-25315062 None:intergenic 25.0%
! TGTAACATAAAGAATCTCTA+TGG - chr4.3:25313663-25313682 None:intergenic 25.0%
!! GTTAAGTCTAGTTCATAAAT+TGG - chr4.3:25314705-25314724 None:intergenic 25.0%
!! TAAGTCTAGTTCATAAATTG+GGG - chr4.3:25314703-25314722 None:intergenic 25.0%
!!! TTACGTTTTTAGTACCTATT+GGG + chr4.3:25314941-25314960 MS.gene47574:CDS 25.0%
AGTAAAGTAGGAACTTACTA+GGG - chr4.3:25315031-25315050 None:intergenic 30.0%
AGTAATTCTATGGATCGATA+CGG - chr4.3:25313793-25313812 None:intergenic 30.0%
ATAAAAGGCTTAGAATATCC+GGG - chr4.3:25313858-25313877 None:intergenic 30.0%
ATAGGTACTAAAAACGTAAC+TGG - chr4.3:25314940-25314959 None:intergenic 30.0%
CATCGTTGTTAGTAATTCTA+TGG - chr4.3:25313803-25313822 None:intergenic 30.0%
GATAAAAGGCTTAGAATATC+CGG - chr4.3:25313859-25313878 None:intergenic 30.0%
!!! GTTACGTTTTTAGTACCTAT+TGG + chr4.3:25314940-25314959 MS.gene47574:CDS 30.0%
AAGAACAATGTGCCATACAA+AGG + chr4.3:25314768-25314787 MS.gene47574:CDS 35.0%
AATGGAAACTTTGAGCTTTG+TGG - chr4.3:25313884-25313903 None:intergenic 35.0%
AGCAATATTGGTGTGAACTA+TGG + chr4.3:25313742-25313761 MS.gene47574:CDS 35.0%
ATAGTCTAGGGGAATATTGT+TGG - chr4.3:25314313-25314332 None:intergenic 35.0%
CAAGTTTCGACATAGCAAAA+AGG - chr4.3:25314422-25314441 None:intergenic 35.0%
CATTTCCTACAAGTAAGTAG+CGG - chr4.3:25314008-25314027 None:intergenic 35.0%
CTAAGCTACAATTCAGAACA+AGG + chr4.3:25314033-25314052 MS.gene47574:CDS 35.0%
CTGTCTAAGAATTTGAGCAT+TGG - chr4.3:25314234-25314253 None:intergenic 35.0%
GAGTAAAGTAGGAACTTACT+AGG - chr4.3:25315032-25315051 None:intergenic 35.0%
GCAAAAGCCAATAGCAATAT+TGG + chr4.3:25313730-25313749 MS.gene47574:CDS 35.0%
GTTCACACCAATATTGCTAT+TGG - chr4.3:25313740-25313759 None:intergenic 35.0%
TAGTCTAGGGGAATATTGTT+GGG - chr4.3:25314312-25314331 None:intergenic 35.0%
TGTAGATTAAGCCACTATCA+GGG - chr4.3:25314368-25314387 None:intergenic 35.0%
TTACTAACAACGATGAAAGC+TGG + chr4.3:25313808-25313827 MS.gene47574:CDS 35.0%
TTGTAATATCCGAAACAGGA+TGG + chr4.3:25314460-25314479 MS.gene47574:CDS 35.0%
TTGTATGGCACATTGTTCTT+TGG - chr4.3:25314768-25314787 None:intergenic 35.0%
TTTCGACATAGCAAAAAGGA+GGG - chr4.3:25314418-25314437 None:intergenic 35.0%
! GCAATTTTTCCTAACTCTAC+AGG + chr4.3:25313706-25313725 MS.gene47574:intron 35.0%
! TTCATGGTTTGGGATCATAA+TGG - chr4.3:25313902-25313921 None:intergenic 35.0%
! TTTTGATGCTTCTCATTGCT+GGG - chr4.3:25314083-25314102 None:intergenic 35.0%
!! AACATCAGAGGCATCAAAAT+TGG + chr4.3:25314120-25314139 MS.gene47574:CDS 35.0%
!! CTTTTTGCTATGTCGAAACT+TGG + chr4.3:25314420-25314439 MS.gene47574:CDS 35.0%
!! TGAGCTTTGTGGTTGATAAA+AGG - chr4.3:25313873-25313892 None:intergenic 35.0%
!! TTGTTCTTTGGAGCTTGTAA+TGG - chr4.3:25314756-25314775 None:intergenic 35.0%
AAAACGTAACTGGCATGAAC+AGG - chr4.3:25314930-25314949 None:intergenic 40.0%
AAACGTAACTGGCATGAACA+GGG - chr4.3:25314929-25314948 None:intergenic 40.0%
AAGGCCATTGAAATAGCAAG+TGG - chr4.3:25314988-25315007 None:intergenic 40.0%
ACCAAAGCATAGCAGATGAA+TGG + chr4.3:25313941-25313960 MS.gene47574:CDS 40.0%
AGGAGCAGATGAAACAGAAT+TGG + chr4.3:25314815-25314834 MS.gene47574:CDS 40.0%
AGTCTAGGGGAATATTGTTG+GGG - chr4.3:25314311-25314330 None:intergenic 40.0%
ATCCCAAACCATGAAATCTC+AGG + chr4.3:25313907-25313926 MS.gene47574:CDS 40.0%
ATGCATACCCATATTTCCCA+TGG + chr4.3:25314277-25314296 MS.gene47574:CDS 40.0%
ATGCCTGAGATTTCATGGTT+TGG - chr4.3:25313913-25313932 None:intergenic 40.0%
CAGAACAAGGTCACAAAATG+TGG + chr4.3:25314046-25314065 MS.gene47574:CDS 40.0%
CAGCTTGTAATATCCGAAAC+AGG + chr4.3:25314456-25314475 MS.gene47574:CDS 40.0%
CATCACACTGTCTCTTATGT+CGG - chr4.3:25314208-25314227 None:intergenic 40.0%
CCAAAGCATAGCAGATGAAT+GGG + chr4.3:25313942-25313961 MS.gene47574:CDS 40.0%
CTAAGACATCCATGGCTAAT+GGG - chr4.3:25314149-25314168 None:intergenic 40.0%
CTCAATTCTCAGAACATCAG+AGG + chr4.3:25314108-25314127 MS.gene47574:CDS 40.0%
CTGTAGATTAAGCCACTATC+AGG - chr4.3:25314369-25314388 None:intergenic 40.0%
CTGTTTCGGATATTACAAGC+TGG - chr4.3:25314458-25314477 None:intergenic 40.0%
GGATATTACAAGCTGGATGT+TGG - chr4.3:25314451-25314470 None:intergenic 40.0%
GTTTCGACATAGCAAAAAGG+AGG - chr4.3:25314419-25314438 None:intergenic 40.0%
TAAAGTGAGAAGAGGAAGGT+TGG - chr4.3:25314615-25314634 None:intergenic 40.0%
TAAGACATCCATGGCTAATG+GGG - chr4.3:25314148-25314167 None:intergenic 40.0%
TACGACGAAAACCAAAAGTC+AGG + chr4.3:25314633-25314652 MS.gene47574:CDS 40.0%
TAGGAACTTACTAGGGTCTT+TGG - chr4.3:25315024-25315043 None:intergenic 40.0%
TGCCTGAGATTTCATGGTTT+GGG - chr4.3:25313912-25313931 None:intergenic 40.0%
TTTCGTCGTAAAGTGAGAAG+AGG - chr4.3:25314623-25314642 None:intergenic 40.0%
! AACAGGGTATTTGGGTAGTA+TGG - chr4.3:25313985-25314004 None:intergenic 40.0%
! ACAGGGTATTTGGGTAGTAT+GGG - chr4.3:25313984-25314003 None:intergenic 40.0%
! ACCTGTTTCTTCTATGTCAC+CGG - chr4.3:25314493-25314512 None:intergenic 40.0%
! CTTTTGATGCTTCTCATTGC+TGG - chr4.3:25314084-25314103 None:intergenic 40.0%
! GGTTCATAGCATTCTTTTCC+AGG - chr4.3:25314897-25314916 None:intergenic 40.0%
! GTTTGCTACATTGCAAGCAT+AGG - chr4.3:25314853-25314872 None:intergenic 40.0%
! TACAAGTAAGTAGCGGAACA+GGG - chr4.3:25314001-25314020 None:intergenic 40.0%
! TCTAAGACATCCATGGCTAA+TGG - chr4.3:25314150-25314169 None:intergenic 40.0%
! TCTGAAGAGAGCATAGTCTA+GGG - chr4.3:25314325-25314344 None:intergenic 40.0%
!! AGAAGAGGAAGGTTGGTATT+GGG - chr4.3:25314608-25314627 None:intergenic 40.0%
!! CTTTGGTTGTTTCTGTAGCA+AGG - chr4.3:25315007-25315026 None:intergenic 40.0%
!! GGTTGATTCTAAGACATCCA+TGG - chr4.3:25314157-25314176 None:intergenic 40.0%
!!! GGCTCCATTAGTTTTGAACT+GGG - chr4.3:25314967-25314986 None:intergenic 40.0%
!!! TGGCTCCATTAGTTTTGAAC+TGG - chr4.3:25314968-25314987 None:intergenic 40.0%
AAAGGAGGGAGTCAAGCATT+TGG - chr4.3:25314404-25314423 None:intergenic 45.0%
AAGGCTTAGAATATCCGGGT+TGG - chr4.3:25313854-25313873 None:intergenic 45.0%
ACTTGTGATGCACTTTCACC+TGG + chr4.3:25314876-25314895 MS.gene47574:CDS 45.0%
AGGTGCAAACATCTACAACG+CGG + chr4.3:25314509-25314528 MS.gene47574:CDS 45.0%
ATGACAGCAAAACCACCCAT+CGG + chr4.3:25314561-25314580 MS.gene47574:CDS 45.0%
CAATGTGCCATACAAAGGCA+AGG + chr4.3:25314773-25314792 MS.gene47574:CDS 45.0%
CACCATACCTTGCCTTTGTA+TGG - chr4.3:25314783-25314802 None:intergenic 45.0%
CACTGTCTCTTATGTCGGAT+CGG - chr4.3:25314203-25314222 None:intergenic 45.0%
CAGCAATGCCTGAGATTTCA+TGG - chr4.3:25313918-25313937 None:intergenic 45.0%
CCCATTCATCTGCTATGCTT+TGG - chr4.3:25313945-25313964 None:intergenic 45.0%
CCTGTGACCATGGGAAATAT+GGG - chr4.3:25314287-25314306 None:intergenic 45.0%
CGACATAAGAGACAGTGTGA+TGG + chr4.3:25314206-25314225 MS.gene47574:CDS 45.0%
CTAGACTATGCTCTCTTCAG+AGG + chr4.3:25314324-25314343 MS.gene47574:CDS 45.0%
CTGTTCCGCTACTTACTTGT+AGG + chr4.3:25314000-25314019 MS.gene47574:CDS 45.0%
GAAAACCAAAAGTCAGGACC+TGG + chr4.3:25314639-25314658 MS.gene47574:CDS 45.0%
GCATGAACAGGGATTGAAAC+TGG - chr4.3:25314918-25314937 None:intergenic 45.0%
GTCGTAAAGTGAGAAGAGGA+AGG - chr4.3:25314619-25314638 None:intergenic 45.0%
TCCGGTGACATAGAAGAAAC+AGG + chr4.3:25314489-25314508 MS.gene47574:CDS 45.0%
TCCTGTGACCATGGGAAATA+TGG - chr4.3:25314288-25314307 None:intergenic 45.0%
TCTATGGATCGATACGGAGA+GGG - chr4.3:25313787-25313806 None:intergenic 45.0%
TCTTCTATGTCACCGGAGTT+TGG - chr4.3:25314486-25314505 None:intergenic 45.0%
TGCCATACAAAGGCAAGGTA+TGG + chr4.3:25314778-25314797 MS.gene47574:CDS 45.0%
TGGGCCCAGTTCAAAACTAA+TGG + chr4.3:25314960-25314979 MS.gene47574:CDS 45.0%
TGGGGCTTATTACACACAGA+TGG + chr4.3:25314675-25314694 MS.gene47574:CDS 45.0%
TTCTATGGATCGATACGGAG+AGG - chr4.3:25313788-25313807 None:intergenic 45.0%
! AAGTAGCGGAACAGGGTATT+TGG - chr4.3:25313994-25314013 None:intergenic 45.0%
! AGTAGCGGAACAGGGTATTT+GGG - chr4.3:25313993-25314012 None:intergenic 45.0%
! CTACAAGTAAGTAGCGGAAC+AGG - chr4.3:25314002-25314021 None:intergenic 45.0%
! CTCTGAAGAGAGCATAGTCT+AGG - chr4.3:25314326-25314345 None:intergenic 45.0%
! CTGAAGAGAGCATAGTCTAG+GGG - chr4.3:25314324-25314343 None:intergenic 45.0%
! GCTTTTGCACCTGTAGAGTT+AGG - chr4.3:25313718-25313737 None:intergenic 45.0%
!! GAGAAGAGGAAGGTTGGTAT+TGG - chr4.3:25314609-25314628 None:intergenic 45.0%
!! GGAGCCACTTGCTATTTCAA+TGG + chr4.3:25314981-25315000 MS.gene47574:CDS 45.0%
!! GTGGTTTTGCTGTCATTCGT+TGG - chr4.3:25314557-25314576 None:intergenic 45.0%
!!! AGTTTTGAACTGGGCCCAAT+AGG - chr4.3:25314958-25314977 None:intergenic 45.0%
AATTGGCACCCCATTAGCCA+TGG + chr4.3:25314137-25314156 MS.gene47574:CDS 50.0%
ACCACAAGAGACCCTGATAG+TGG + chr4.3:25314354-25314373 MS.gene47574:CDS 50.0%
ACCTGGAACAGAACGACACT+GGG + chr4.3:25314656-25314675 MS.gene47574:CDS 50.0%
CAAAGGCAAGGTATGGTGTG+TGG + chr4.3:25314785-25314804 MS.gene47574:CDS 50.0%
CCCATATTTCCCATGGTCAC+AGG + chr4.3:25314284-25314303 MS.gene47574:CDS 50.0%
GAAACAGGATGGCCAAACTC+CGG + chr4.3:25314471-25314490 MS.gene47574:CDS 50.0%
GATCGGAATGTTCCGCTTGA+CGG - chr4.3:25314186-25314205 None:intergenic 50.0%
GCATAGCAGATGAATGGGTG+AGG + chr4.3:25313947-25313966 MS.gene47574:CDS 50.0%
GGAGTTTGGCCATCCTGTTT+CGG - chr4.3:25314472-25314491 None:intergenic 50.0%
GGGTATTTGGGTAGTATGGG+AGG - chr4.3:25313981-25314000 None:intergenic 50.0%
GTGTGAACTATGGCGTGCTT+GGG + chr4.3:25313752-25313771 MS.gene47574:CDS 50.0%
! CTGTTCCAGGTCCTGACTTT+TGG - chr4.3:25314647-25314666 None:intergenic 50.0%
CCTGGAACAGAACGACACTG+GGG + chr4.3:25314657-25314676 MS.gene47574:CDS 55.0%
GACCTGGAACAGAACGACAC+TGG + chr4.3:25314655-25314674 MS.gene47574:CDS 55.0%
GCCACTATCAGGGTCTCTTG+TGG - chr4.3:25314358-25314377 None:intergenic 55.0%
GGAATGTTCCGCTTGACGGT+GGG - chr4.3:25314182-25314201 None:intergenic 55.0%
GGTGTGAACTATGGCGTGCT+TGG + chr4.3:25313751-25313770 MS.gene47574:CDS 55.0%
GTATGGTGTGTGGCTGCAGA+AGG + chr4.3:25314795-25314814 MS.gene47574:CDS 55.0%
TCAACCTTCCCACCGTCAAG+CGG + chr4.3:25314171-25314190 MS.gene47574:CDS 55.0%
TGGCGTCGTAGAGCTTCACA+CGG - chr4.3:25313834-25313853 None:intergenic 55.0%
TGTTGGGGTCCTGTGACCAT+GGG - chr4.3:25314296-25314315 None:intergenic 55.0%
TTGTTGGGGTCCTGTGACCA+TGG - chr4.3:25314297-25314316 None:intergenic 55.0%
!! AAGGTTGGTATTGGGACGCC+AGG - chr4.3:25314600-25314619 None:intergenic 55.0%
!! AGGTTGGTATTGGGACGCCA+GGG - chr4.3:25314599-25314618 None:intergenic 55.0%
CGGAATGTTCCGCTTGACGG+TGG - chr4.3:25314183-25314202 None:intergenic 60.0%
GAAGCTCTACGACGCCAACC+CGG + chr4.3:25313837-25313856 MS.gene47574:CDS 60.0%
TGTTCCGCTTGACGGTGGGA+AGG - chr4.3:25314178-25314197 None:intergenic 60.0%
! CCCCAGTGTCGTTCTGTTCC+AGG - chr4.3:25314660-25314679 None:intergenic 60.0%
AGGGCGTGCTGGGGTTCCGA+TGG - chr4.3:25314580-25314599 None:intergenic 70.0%
ATCGGAACCCCAGCACGCCC+TGG + chr4.3:25314579-25314598 MS.gene47574:CDS 70.0%
ATTGGGACGCCAGGGCGTGC+TGG - chr4.3:25314591-25314610 None:intergenic 70.0%
CGTGCTGGGGTTCCGATGGG+TGG - chr4.3:25314576-25314595 None:intergenic 70.0%
GGGCGTGCTGGGGTTCCGAT+GGG - chr4.3:25314579-25314598 None:intergenic 70.0%
TTGGGACGCCAGGGCGTGCT+GGG - chr4.3:25314590-25314609 None:intergenic 70.0%
TGGGACGCCAGGGCGTGCTG+GGG - chr4.3:25314589-25314608 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 25313603 25315175 25313603 ID=MS.gene47574
chr4.3 mRNA 25313603 25315175 25313603 ID=MS.gene47574.t1;Parent=MS.gene47574
chr4.3 exon 25313603 25313648 25313603 ID=MS.gene47574.t1.exon1;Parent=MS.gene47574.t1
chr4.3 CDS 25313603 25313648 25313603 ID=cds.MS.gene47574.t1;Parent=MS.gene47574.t1
chr4.3 exon 25313728 25315032 25313728 ID=MS.gene47574.t1.exon2;Parent=MS.gene47574.t1
chr4.3 CDS 25313728 25315032 25313728 ID=cds.MS.gene47574.t1;Parent=MS.gene47574.t1
chr4.3 exon 25315138 25315175 25315138 ID=MS.gene47574.t1.exon3;Parent=MS.gene47574.t1
chr4.3 CDS 25315138 25315175 25315138 ID=cds.MS.gene47574.t1;Parent=MS.gene47574.t1
Gene Sequence

>MS.gene47574

ATGGATTATCTGGCTATATTTTTAGTCTGTTTAATTTCATTATCAAGTAAGTTTGAGCCATAGAGATTCTTTATGTTACATTGTCTTATTACATTAAACTAATGCAATTTTTCCTAACTCTACAGGTGCAAAAGCCAATAGCAATATTGGTGTGAACTATGGCGTGCTTGGGAACAATCTACCCTCTCCGTATCGATCCATAGAATTACTAACAACGATGAAAGCTGGCCGTGTGAAGCTCTACGACGCCAACCCGGATATTCTAAGCCTTTTATCAACCACAAAGCTCAAAGTTTCCATTATGATCCCAAACCATGAAATCTCAGGCATTGCTGCAAACCAAAGCATAGCAGATGAATGGGTGAGGAACAATATCCTCCCATACTACCCAAATACCCTGTTCCGCTACTTACTTGTAGGAAATGAAGTGCTAAGCTACAATTCAGAACAAGGTCACAAAATGTGGCATGATCTTGTCCCAGCAATGAGAAGCATCAAAAGAGCACTCAATTCTCAGAACATCAGAGGCATCAAAATTGGCACCCCATTAGCCATGGATGTCTTAGAATCAACCTTCCCACCGTCAAGCGGAACATTCCGATCCGACATAAGAGACAGTGTGATGGCACCAATGCTCAAATTCTTAGACAGAACCAATTCATTTTTCTTCATTGATGCATACCCATATTTCCCATGGTCACAGGACCCCAACAATATTCCCCTAGACTATGCTCTCTTCAGAGGAGATTTCACCACAAGAGACCCTGATAGTGGCTTAATCTACAGAAACTTGTTAGACCAAATGCTTGACTCCCTCCTTTTTGCTATGTCGAAACTTGGATACTCCAACATCCAGCTTGTAATATCCGAAACAGGATGGCCAAACTCCGGTGACATAGAAGAAACAGGTGCAAACATCTACAACGCGGCTACATACAATCGCAATCTCATCCAACGAATGACAGCAAAACCACCCATCGGAACCCCAGCACGCCCTGGCGTCCCAATACCAACCTTCCTCTTCTCACTTTACGACGAAAACCAAAAGTCAGGACCTGGAACAGAACGACACTGGGGCTTATTACACACAGATGGAACCCCAATTTATGAACTAGACTTAACGGGTAAAAAAAATAACACAGATTATGCACCATTACAAGCTCCAAAGAACAATGTGCCATACAAAGGCAAGGTATGGTGTGTGGCTGCAGAAGGAGCAGATGAAACAGAATTGGCAGCAGCTTTGACCTATGCTTGCAATGTAGCAAACATGACTTGTGATGCACTTTCACCTGGAAAAGAATGCTATGAACCAGTTTCAATCCCTGTTCATGCCAGTTACGTTTTTAGTACCTATTGGGCCCAGTTCAAAACTAATGGAGCCACTTGCTATTTCAATGGCCTTGCTACAGAAACAACCAAAGACCCTAGTAAGTTCCTACTTTACTCTCTTTACAATATAATCATAGTTTCACAAAGTTATAATTACAAATTTCATTTCAATCAATAACCTAATGTAATTGTTTATGTGACAGGTCGAAATTCTTGCAAATTCCCAAGCGTAATTCTCTAG
Protein sequence

>MS.gene47574.t1

MDYLAIFLVCLISLSSAKANSNIGVNYGVLGNNLPSPYRSIELLTTMKAGRVKLYDANPDILSLLSTTKLKVSIMIPNHEISGIAANQSIADEWVRNNILPYYPNTLFRYLLVGNEVLSYNSEQGHKMWHDLVPAMRSIKRALNSQNIRGIKIGTPLAMDVLESTFPPSSGTFRSDIRDSVMAPMLKFLDRTNSFFFIDAYPYFPWSQDPNNIPLDYALFRGDFTTRDPDSGLIYRNLLDQMLDSLLFAMSKLGYSNIQLVISETGWPNSGDIEETGANIYNAATYNRNLIQRMTAKPPIGTPARPGVPIPTFLFSLYDENQKSGPGTERHWGLLHTDGTPIYELDLTGKKNNTDYAPLQAPKNNVPYKGKVWCVAAEGADETELAAALTYACNVANMTCDALSPGKECYEPVSIPVHASYVFSTYWAQFKTNGATCYFNGLATETTKDPSRNSCKFPSVIL