AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene008862

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008862.t1 MTR_8g069575 97.080 411 12 0 1 411 1 411 0.0 842
MS.gene008862.t1 MTR_5g033650 78.022 364 78 2 49 411 4 366 0.0 595
MS.gene008862.t1 MTR_3g077430 69.452 383 114 3 30 411 85 465 0.0 574
MS.gene008862.t1 MTR_8g069610 64.493 414 144 1 1 411 1 414 0.0 561
MS.gene008862.t1 MTR_4g105400 71.920 349 95 2 65 411 11 358 0.0 541
MS.gene008862.t1 MTR_8g069600 63.309 417 123 3 1 411 1 393 0.0 535
MS.gene008862.t1 MTR_3g095890 63.133 415 136 6 1 410 31 433 0.0 526
MS.gene008862.t1 MTR_8g069720 58.513 417 157 6 1 411 1 407 5.76e-179 504
MS.gene008862.t1 MTR_8g069580 58.711 419 140 8 1 411 1 394 3.11e-170 482
MS.gene008862.t1 MTR_7g116420 57.772 386 139 4 49 410 54 439 4.70e-164 468
MS.gene008862.t1 MTR_8g069640 55.556 414 170 4 1 410 1 404 2.12e-162 462
MS.gene008862.t1 MTR_8g069660 55.181 415 167 6 1 411 1 400 2.30e-160 457
MS.gene008862.t1 MTR_1g079730 51.810 442 179 6 3 411 11 451 1.57e-159 457
MS.gene008862.t1 MTR_8g088510 53.682 421 165 6 8 410 7 415 2.95e-159 455
MS.gene008862.t1 MTR_2g062610 53.627 386 158 3 46 411 42 426 6.22e-155 444
MS.gene008862.t1 MTR_8g069590 64.169 307 107 3 106 411 1 305 6.79e-144 412
MS.gene008862.t1 MTR_8g069715 50.839 417 173 6 1 411 20 410 7.40e-142 410
MS.gene008862.t1 MTR_2g062610 57.143 308 121 2 114 411 30 336 2.88e-135 391
MS.gene008862.t1 MTR_2g062610 57.143 308 121 2 114 411 37 343 3.96e-135 391
MS.gene008862.t1 MTR_2g073040 64.074 270 90 3 20 286 4 269 7.21e-123 357
MS.gene008862.t1 MTR_2g073020 60.777 283 100 4 11 286 4 282 8.52e-118 344
MS.gene008862.t1 MTR_2g093840 57.632 321 120 8 95 411 52 360 4.26e-116 343
MS.gene008862.t1 MTR_6g012620 41.117 394 191 10 49 411 27 410 7.00e-97 296
MS.gene008862.t1 MTR_3g104000 38.636 396 215 7 42 411 24 417 2.11e-89 277
MS.gene008862.t1 MTR_3g103980 37.413 433 231 11 3 411 19 435 2.23e-89 277
MS.gene008862.t1 MTR_3g104030 39.844 384 205 8 45 407 32 410 2.94e-89 276
MS.gene008862.t1 MTR_3g104070 37.002 427 225 11 7 406 7 416 4.03e-88 273
MS.gene008862.t1 MTR_8g069695 48.800 250 123 3 164 411 13 259 4.72e-83 255
MS.gene008862.t1 MTR_1g016910 34.803 431 240 8 9 411 3 420 1.88e-76 243
MS.gene008862.t1 MTR_3g104030 39.564 321 161 7 113 407 33 346 1.87e-70 226
MS.gene008862.t1 MTR_5g078580 33.933 389 225 12 7 388 4 367 1.04e-55 189
MS.gene008862.t1 MTR_8g040850 32.048 415 243 12 7 407 1 390 1.68e-54 186
MS.gene008862.t1 MTR_8g069630 63.846 130 45 2 283 411 1 129 1.79e-53 174
MS.gene008862.t1 MTR_8g040890 31.002 429 250 12 12 405 1 418 1.21e-51 179
MS.gene008862.t1 MTR_8g069750 61.607 112 43 0 219 330 151 262 4.07e-45 157
MS.gene008862.t1 MTR_5g092260 28.224 411 255 13 3 407 8 384 2.02e-41 150
MS.gene008862.t1 MTR_5g094280 34.164 281 161 9 39 314 17 278 2.84e-41 149
MS.gene008862.t1 MTR_3g108680 29.330 358 225 13 58 407 49 386 7.16e-40 146
MS.gene008862.t1 MTR_2g046390 30.618 356 210 11 63 405 37 368 9.37e-39 143
MS.gene008862.t1 MTR_8g104480 30.423 355 199 14 73 405 9 337 1.31e-37 139
MS.gene008862.t1 MTR_8g012985 37.083 240 135 9 176 405 4 237 1.90e-37 136
MS.gene008862.t1 MTR_2g046520 34.979 243 143 9 175 407 3 240 1.21e-33 126
MS.gene008862.t1 MTR_2g046430 34.979 243 143 9 175 407 3 240 4.23e-33 124
MS.gene008862.t1 MTR_8g069665 56.481 108 45 2 44 150 86 192 6.70e-33 123
MS.gene008862.t1 MTR_2g046300 28.804 368 186 14 63 405 37 353 7.32e-30 119
MS.gene008862.t1 MTR_2g073050 71.831 71 19 1 341 411 1 70 2.85e-29 108
MS.gene008862.t1 MTR_5g065260 31.897 232 149 5 176 405 2 226 6.07e-28 110
MS.gene008862.t1 MTR_8g069950 80.000 55 11 0 238 292 58 112 1.47e-23 95.1
MS.gene008862.t1 MTR_0009s0020 36.957 138 81 4 220 357 18 149 4.84e-22 92.0
MS.gene008862.t1 MTR_7g072230 32.000 175 107 6 238 403 34 205 5.72e-21 90.9
MS.gene008862.t1 MTR_2g046390 24.859 354 190 12 63 405 37 325 5.53e-19 87.8
MS.gene008862.t1 MTR_8g069585 53.425 73 30 2 11 80 6 77 4.18e-17 77.4
MS.gene008862.t1 MTR_5g065920 55.357 56 23 1 165 220 9 62 1.12e-15 72.0
MS.gene008862.t1 MTR_8g064210 48.052 77 36 2 2 74 15 91 2.32e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene008862.t1 AT1G10750 73.560 382 100 1 30 411 87 467 0.0 611
MS.gene008862.t1 AT1G23340 67.561 410 131 2 3 411 1 409 0.0 588
MS.gene008862.t1 AT1G23340 67.561 410 131 2 3 411 1 409 0.0 588
MS.gene008862.t1 AT1G70550 70.712 379 110 1 33 411 88 465 0.0 582
MS.gene008862.t1 AT5G50150 69.634 382 113 3 32 411 40 420 0.0 569
MS.gene008862.t1 AT1G55360 55.699 386 152 5 44 411 38 422 3.30e-158 452
MS.gene008862.t1 AT3G13510 52.506 419 168 7 10 411 15 419 7.20e-158 451
MS.gene008862.t1 AT5G18460 56.250 384 142 5 49 410 50 429 9.52e-158 452
MS.gene008862.t1 AT5G56530 52.706 425 157 8 10 410 15 419 1.18e-153 441
MS.gene008862.t1 AT5G56530 52.706 425 157 8 10 410 15 419 1.18e-153 441
MS.gene008862.t1 AT2G44210 51.074 419 175 7 13 411 7 415 6.23e-149 429
MS.gene008862.t1 AT2G44210 47.884 449 174 8 13 411 7 445 4.65e-143 415
MS.gene008862.t1 AT1G23340 65.965 285 95 2 3 286 1 284 2.03e-136 392
MS.gene008862.t1 AT2G44220 47.816 412 186 9 14 411 7 403 6.23e-133 387
MS.gene008862.t1 AT2G44240 49.474 380 181 7 40 411 26 402 4.78e-124 365
MS.gene008862.t1 AT2G17750 41.849 411 212 10 10 410 2 395 1.80e-113 338
MS.gene008862.t1 AT3G48230 48.000 375 183 8 45 411 3 373 6.58e-113 335
MS.gene008862.t1 AT2G44250 45.146 412 204 11 11 410 7 408 5.20e-110 329
MS.gene008862.t1 AT2G19360 41.995 431 205 10 10 410 10 425 1.27e-108 326
MS.gene008862.t1 AT5G19170 46.067 356 168 7 61 405 23 365 1.00e-104 315
MS.gene008862.t1 AT1G70550 69.268 205 62 1 33 237 88 291 1.56e-102 306
MS.gene008862.t1 AT1G10750 61.404 228 86 2 30 256 87 313 4.07e-95 289
MS.gene008862.t1 AT2G44250 48.860 307 144 7 114 410 22 325 5.92e-89 273
MS.gene008862.t1 AT5G25950 36.842 437 229 9 5 411 13 432 1.84e-81 257
MS.gene008862.t1 AT5G25960 37.500 408 196 8 12 411 1 357 8.67e-74 234
MS.gene008862.t1 AT2G20170 36.983 411 221 15 10 405 9 396 5.51e-73 234
MS.gene008862.t1 AT2G20170 36.983 411 221 15 10 405 9 396 5.51e-73 234
MS.gene008862.t1 AT4G23390 37.228 368 205 13 53 405 41 397 5.63e-66 216
MS.gene008862.t1 AT4G23370 35.013 377 220 12 46 405 33 401 7.83e-66 216
MS.gene008862.t1 AT2G20170 34.793 411 213 15 10 405 9 379 2.99e-64 211
MS.gene008862.t1 AT4G23380 33.090 411 242 13 9 405 7 398 2.33e-62 206
MS.gene008862.t1 AT4G23350 31.452 372 236 9 46 405 36 400 1.21e-58 197
MS.gene008862.t1 AT4G23365 31.989 372 232 11 46 405 36 398 1.77e-58 196
MS.gene008862.t1 AT4G23355 32.000 375 228 11 46 405 36 398 9.74e-57 192
MS.gene008862.t1 AT4G23360 32.361 377 225 12 46 405 36 399 2.00e-55 188
MS.gene008862.t1 AT2G35250 33.724 341 198 9 70 407 24 339 1.47e-51 176
MS.gene008862.t1 AT1G10190 31.159 414 240 20 15 405 3 394 1.03e-44 160
MS.gene008862.t1 AT4G17505 30.141 355 193 13 61 411 18 321 1.59e-44 157
MS.gene008862.t1 AT4G15050 30.504 377 222 17 49 405 38 394 6.91e-41 149
MS.gene008862.t1 AT2G27320 33.663 303 170 9 72 370 30 305 1.82e-39 144
MS.gene008862.t1 AT2G24950 28.993 407 248 17 14 405 29 409 2.20e-38 143
MS.gene008862.t1 AT4G15053 29.535 430 249 20 1 405 1 401 1.06e-36 138
MS.gene008862.t1 AT4G10220 27.446 419 255 15 6 405 10 398 4.35e-36 137
MS.gene008862.t1 AT4G23373 34.263 251 133 9 9 258 6 225 6.45e-34 129
MS.gene008862.t1 AT5G05030 29.167 360 224 12 57 407 22 359 5.68e-33 127
MS.gene008862.t1 AT5G46810 27.714 350 225 11 73 405 11 349 1.31e-32 126
MS.gene008862.t1 AT5G46820 28.451 355 226 12 64 405 8 347 1.65e-32 126
MS.gene008862.t1 AT5G25410 27.500 360 226 13 57 405 28 363 2.81e-31 123
MS.gene008862.t1 AT5G60380 28.571 343 216 13 73 405 44 367 1.92e-29 118
MS.gene008862.t1 AT4G10210 26.392 413 250 15 1 390 5 386 6.04e-29 117
MS.gene008862.t1 AT5G46200 27.013 385 219 15 73 405 31 405 1.14e-28 116
MS.gene008862.t1 AT2G38255 33.175 211 105 6 73 275 14 196 2.89e-28 111
MS.gene008862.t1 AT5G11660 31.058 293 180 11 123 407 26 304 2.89e-26 107
MS.gene008862.t1 AT4G23080 27.400 427 255 20 6 407 10 406 4.65e-26 109
MS.gene008862.t1 AT4G17860 28.977 352 202 17 73 405 29 351 2.30e-25 106
MS.gene008862.t1 AT5G25415 32.787 244 143 8 175 407 99 332 1.37e-24 103
MS.gene008862.t1 AT5G36680 28.383 303 181 12 122 410 51 331 3.00e-23 100
MS.gene008862.t1 AT5G36770 28.383 303 181 12 122 410 51 331 3.00e-23 100
MS.gene008862.t1 AT2G03930 52.577 97 43 3 160 253 38 134 3.34e-23 95.9
MS.gene008862.t1 AT5G04937 29.442 197 112 8 216 405 22 198 4.43e-17 79.7
MS.gene008862.t1 AT5G37520 28.017 232 126 11 181 406 2 198 4.93e-15 73.9

Find 111 sgRNAs with CRISPR-Local

Find 255 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCCTAGATTCTTCACTTAT+TGG 0.171239 8.1:+35460352 MS.gene008862:CDS
ATCCCTATTCACACACTTAA+AGG 0.191236 8.1:+35462182 MS.gene008862:CDS
TCTTGTATATTATAACAATT+TGG 0.235227 8.1:-35462402 None:intergenic
ATGAAGAGTTTCACAAGTTT+AGG 0.287405 8.1:+35458087 MS.gene008862:CDS
TCTCCAACTTCTACATATAA+TGG 0.288121 8.1:+35460601 MS.gene008862:CDS
AATTCAGCTTGTCTCAAATA+TGG 0.288451 8.1:+35459769 MS.gene008862:CDS
CCCATTTGCTTATGCTCTCT+TGG 0.302334 8.1:-35458467 None:intergenic
ATACAAGAAGGAACTAATAA+TGG 0.313088 8.1:+35462417 MS.gene008862:CDS
TTGGTAGAAAAGTTTGATTT+TGG 0.315468 8.1:-35458064 None:intergenic
CAAAATGTCCACTACCCATT+TGG 0.328159 8.1:-35462275 None:intergenic
AGCCACCAATTCCCATGCTT+TGG 0.330284 8.1:-35462120 None:intergenic
GGGAATTGGTGGCTTGAATA+TGG 0.338331 8.1:+35462129 MS.gene008862:CDS
TGGATCATCTCTGGTTCATT+TGG 0.338622 8.1:+35459789 MS.gene008862:CDS
CTAATCGGAATTGTTCCTTC+TGG 0.344077 8.1:-35458539 None:intergenic
GCTTGCTCTATTAACACATT+TGG 0.344553 8.1:+35458590 MS.gene008862:CDS
AAACAAGGAGAAGAAAATAA+AGG 0.350929 8.1:-35458004 None:intergenic
AGTGCAACAATGGTTCAATT+TGG 0.351443 8.1:+35462207 MS.gene008862:CDS
TATCCTTTAAGTGTGTGAAT+AGG 0.369475 8.1:-35462185 None:intergenic
TTAGCTACAACATTGAGATT+TGG 0.370876 8.1:-35462375 None:intergenic
TGCTACAATTTACTTTGCTC+TGG 0.378764 8.1:+35460535 MS.gene008862:CDS
GTCCAATAAGTGAAGAATCT+AGG 0.380593 8.1:-35460354 None:intergenic
ATCCTTTAAGTGTGTGAATA+GGG 0.384997 8.1:-35462184 None:intergenic
GTCTCACACTTCTACCCAAA+TGG 0.386568 8.1:+35462260 MS.gene008862:CDS
ACTAGCAGTAATATTGCAAT+TGG 0.395249 8.1:+35460568 MS.gene008862:CDS
TTGATATTAGCTTAATGATC+TGG 0.397300 8.1:+35460632 MS.gene008862:CDS
ACTGATGCATATCAAGGAAC+TGG 0.402596 8.1:+35460511 MS.gene008862:CDS
AACTTGTGAAACTCTTCATT+TGG 0.403131 8.1:-35458083 None:intergenic
ATAGTGAATACAATGTCAAC+AGG 0.406411 8.1:+35462237 MS.gene008862:CDS
CAGGTCAGCCCAGAGCTCTA+TGG 0.408690 8.1:+35460321 MS.gene008862:intron
TATGGATCTGGAAACTTGAT+TGG 0.410366 8.1:+35462147 MS.gene008862:CDS
TCATTAAGCTAATATCAAAT+TGG 0.410645 8.1:-35460627 None:intergenic
CTGGAAACTTGATTGGGTAT+TGG 0.411401 8.1:+35462154 MS.gene008862:CDS
TTTCAATTGTGGAGTTTCTC+AGG 0.412909 8.1:+35458506 MS.gene008862:CDS
AAAATGTCCACTACCCATTT+GGG 0.415939 8.1:-35462274 None:intergenic
TGAGACAAGCTGAATTCATA+TGG 0.421304 8.1:-35459762 None:intergenic
AAAGGAGGAGGGAGATGACT+GGG 0.428087 8.1:-35457986 None:intergenic
ACCAAGAGAGCATAAGCAAA+TGG 0.428963 8.1:+35458466 MS.gene008862:CDS
CATATGGACTTTCCACTTGT+GGG 0.431251 8.1:-35459746 None:intergenic
GCCTGCTGTTAAGACAATTC+AGG 0.432132 8.1:+35458145 MS.gene008862:CDS
AAAATTGAAACGTGTGAGAA+TGG 0.438872 8.1:+35458616 MS.gene008862:CDS
TACAAGAAGGAACTAATAAT+GGG 0.448624 8.1:+35462418 MS.gene008862:CDS
TAAAGGAGGAGGGAGATGAC+TGG 0.452466 8.1:-35457987 None:intergenic
TAGCTATCCCCATAGAGCTC+TGG 0.454983 8.1:-35460330 None:intergenic
GGAGAAGAAAATAAAGGAGG+AGG 0.457366 8.1:-35457998 None:intergenic
TACCATTGAAGCTGGTTGGC+AGG 0.466050 8.1:+35459824 MS.gene008862:CDS
CCTCCATTATATGTAGAAGT+TGG 0.467832 8.1:-35460604 None:intergenic
AGCTATCCCCATAGAGCTCT+GGG 0.473291 8.1:-35460329 None:intergenic
TGGTGGCTTGAATATGGATC+TGG 0.475485 8.1:+35462135 MS.gene008862:CDS
AGGATCCAAAGCATGGGAAT+TGG 0.479599 8.1:+35462115 MS.gene008862:intron
TGCCTGCCAACCAGCTTCAA+TGG 0.481927 8.1:-35459826 None:intergenic
AGGTCAGCCCAGAGCTCTAT+GGG 0.482474 8.1:+35460322 MS.gene008862:intron
ACCTGAATTGTCTTAACAGC+AGG 0.484466 8.1:-35458146 None:intergenic
GCAACAATGGTTCAATTTGG+TGG 0.484648 8.1:+35462210 MS.gene008862:CDS
GTGAGTGGGAATGAGTACTA+TGG 0.487457 8.1:+35459696 MS.gene008862:CDS
AAAATTATAACTTTCAATTG+TGG 0.487536 8.1:+35458495 MS.gene008862:CDS
TCTCAAATATGGATCATCTC+TGG 0.501761 8.1:+35459780 MS.gene008862:CDS
CTTTAGAAATATGCAAGTTG+TGG 0.502120 8.1:+35462326 MS.gene008862:CDS
TCCAATAAGTGAAGAATCTA+GGG 0.502742 8.1:-35460353 None:intergenic
CCAAGAGAGCATAAGCAAAT+GGG 0.509356 8.1:+35458467 MS.gene008862:CDS
TTATGCTCTCTTGGTCTCTC+CGG 0.511099 8.1:-35458458 None:intergenic
TCATATGGACTTTCCACTTG+TGG 0.512196 8.1:-35459747 None:intergenic
GAGAAGAAAATAAAGGAGGA+GGG 0.526498 8.1:-35457997 None:intergenic
TCTCACACTTCTACCCAAAT+GGG 0.527784 8.1:+35462261 MS.gene008862:CDS
ATCCAAAGCATGGGAATTGG+TGG 0.528426 8.1:+35462118 MS.gene008862:CDS
ATGGATCTGGAAACTTGATT+GGG 0.531742 8.1:+35462148 MS.gene008862:CDS
AACATAGGATGGTCAAAAGC+TGG 0.536748 8.1:-35458306 None:intergenic
TTGCAGACTGATGCATATCA+AGG 0.537288 8.1:+35460505 MS.gene008862:intron
TTTATGTGTATAGGATCCTC+CGG 0.539657 8.1:+35458439 MS.gene008862:intron
CAAGGAGAAGAAAATAAAGG+AGG 0.541972 8.1:-35458001 None:intergenic
AAGAGTTTCACAAGTTTAGG+AGG 0.546074 8.1:+35458090 MS.gene008862:CDS
GAAGGAACTAATAATGGGTG+GGG 0.546366 8.1:+35462423 MS.gene008862:CDS
TTGTACCTCATGTCCATCGC+CGG 0.547979 8.1:-35458645 None:intergenic
GATCTCAATACCATTGAAGC+TGG 0.548557 8.1:+35459816 MS.gene008862:CDS
TCAATACCATTGAAGCTGGT+TGG 0.549014 8.1:+35459820 MS.gene008862:CDS
TTTGTCCTCTCAACATAGGA+TGG 0.550432 8.1:-35458317 None:intergenic
CAGTCTATGATATCACCATC+AGG 0.554468 8.1:-35458264 None:intergenic
AGAAGGAACTAATAATGGGT+GGG 0.554565 8.1:+35462422 MS.gene008862:CDS
AGAATGGACACCACCGGCGA+TGG 0.558764 8.1:+35458632 MS.gene008862:CDS
TACAACATTGAGATTTGGCA+AGG 0.560466 8.1:-35462370 None:intergenic
TGCGTGTAGGATCCAAAGCA+TGG 0.565093 8.1:+35462108 MS.gene008862:intron
AAATGTGTGGGTCCCACAAG+TGG 0.566747 8.1:+35459734 MS.gene008862:CDS
GTATATTATAACAATTTGGA+CGG 0.567975 8.1:-35462398 None:intergenic
TAGATTCTTCACTTATTGGA+CGG 0.568545 8.1:+35460356 MS.gene008862:CDS
TTTACTATGGTGGTCCTGGA+AGG 0.570062 8.1:+35462454 MS.gene008862:CDS
ACTTCTACCCAAATGGGTAG+TGG 0.571003 8.1:+35462267 MS.gene008862:CDS
AATGATGCACAGCATGCAGT+TGG 0.572287 8.1:+35459669 MS.gene008862:intron
CTTAAAGGATAGTGCAACAA+TGG 0.576183 8.1:+35462197 MS.gene008862:CDS
TGCTCTCTTGGTCTCTCCGG+AGG 0.583271 8.1:-35458455 None:intergenic
CCGATTAGAAGAATAAAAGA+AGG 0.583646 8.1:+35458554 MS.gene008862:CDS
CAAAAGCTAGCATAAATGTG+TGG 0.585357 8.1:+35459721 MS.gene008862:CDS
AATTGTTATAATATACAAGA+AGG 0.590507 8.1:+35462405 MS.gene008862:CDS
TTTGACCATCCTATGTTGAG+AGG 0.592755 8.1:+35458312 MS.gene008862:CDS
GTTTATTCTTCAGAAACAAG+TGG 0.596638 8.1:+35458041 MS.gene008862:CDS
CACCACCGGCGATGGACATG+AGG 0.599402 8.1:+35458640 MS.gene008862:CDS
CTGATGCATATCAAGGAACT+GGG 0.602205 8.1:+35460512 MS.gene008862:CDS
AAGAAGGAACTAATAATGGG+TGG 0.603081 8.1:+35462421 MS.gene008862:CDS
AAGTGTGTGAATAGGGATGA+TGG 0.604788 8.1:-35462177 None:intergenic
TACCTCATGTCCATCGCCGG+TGG 0.612456 8.1:-35458642 None:intergenic
AAAAGCTAGCATAAATGTGT+GGG 0.619969 8.1:+35459722 MS.gene008862:CDS
TATTAGCTTAATGATCTGGA+AGG 0.620476 8.1:+35460636 MS.gene008862:CDS
CATGACAAACCAAAGAAACA+AGG 0.620517 8.1:-35458019 None:intergenic
TCAGGTGAATCATGTCCAGA+AGG 0.626410 8.1:+35458524 MS.gene008862:CDS
GCGTGTAGGATCCAAAGCAT+GGG 0.628244 8.1:+35462109 MS.gene008862:intron
ACAACATTGAGATTTGGCAA+GGG 0.654257 8.1:-35462369 None:intergenic
AAGGAGGAGGGAGATGACTG+GGG 0.658931 8.1:-35457985 None:intergenic
CCAACTTCTACATATAATGG+AGG 0.662547 8.1:+35460604 MS.gene008862:CDS
ATGCAGTTGGATATGTGAGT+GGG 0.674700 8.1:+35459682 MS.gene008862:CDS
CATGCAGTTGGATATGTGAG+TGG 0.677188 8.1:+35459681 MS.gene008862:CDS
CGTGTGAGAATGGACACCAC+CGG 0.706037 8.1:+35458626 MS.gene008862:CDS
TAGTGAATACAATGTCAACA+GGG 0.716345 8.1:+35462238 MS.gene008862:CDS
GGTCAGCCCAGAGCTCTATG+GGG 0.745363 8.1:+35460323 MS.gene008862:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATATGATAAATTATG+TGG - chr8.1:35461574-35461593 None:intergenic 10.0%
!! TGTATTTAAAAAAATGATAA+TGG + chr8.1:35460472-35460491 MS.gene008862:intron 10.0%
!!! AAATGATTTTTTTTTTTTGA+AGG + chr8.1:35460098-35460117 MS.gene008862:intron 10.0%
!!! TCTTTTGAAATAATTAATAT+CGG + chr8.1:35459190-35459209 MS.gene008862:intron 10.0%
!!! TCTTTTTTAAACTATTAAAA+AGG + chr8.1:35461983-35462002 MS.gene008862:intron 10.0%
!!! TTTTATCAGAATATTATTTT+TGG - chr8.1:35459400-35459419 None:intergenic 10.0%
!!! TTTTTTGAAGAAAAAATAAA+TGG + chr8.1:35458201-35458220 MS.gene008862:intron 10.0%
!! AAAAATGAAGGAATAAAAAA+AGG - chr8.1:35461687-35461706 None:intergenic 15.0%
!! AAAATTATAACTTTCAATTG+TGG + chr8.1:35458495-35458514 MS.gene008862:CDS 15.0%
!! AAAGAAAATATAGACAAAAA+TGG - chr8.1:35458987-35459006 None:intergenic 15.0%
!! AAATTGATTATAAATGTGAT+CGG - chr8.1:35460755-35460774 None:intergenic 15.0%
!! AATTGTTATAATATACAAGA+AGG + chr8.1:35462405-35462424 MS.gene008862:CDS 15.0%
!! ATTATTTCAAAAGAAATGAA+TGG - chr8.1:35459184-35459203 None:intergenic 15.0%
!! ATTTATATTAGATTCAATGT+TGG - chr8.1:35459600-35459619 None:intergenic 15.0%
!! CTATTGAAGATAAAAATAAT+TGG - chr8.1:35459553-35459572 None:intergenic 15.0%
!! TATTTCAACTTATAATTGTT+CGG + chr8.1:35458415-35458434 MS.gene008862:intron 15.0%
!! TCTTGTATATTATAACAATT+TGG - chr8.1:35462405-35462424 None:intergenic 15.0%
!! TGAAAAAAAGAAACAAAAAA+GGG - chr8.1:35461551-35461570 None:intergenic 15.0%
!! TTAAAAATTGTTTATAAACG+TGG + chr8.1:35460857-35460876 MS.gene008862:intron 15.0%
!!! AAAGTATCATTATTAAGTAT+TGG - chr8.1:35460073-35460092 None:intergenic 15.0%
!!! AATATTTTGTAACAAATCAA+TGG - chr8.1:35461133-35461152 None:intergenic 15.0%
!!! ATTTTTCGTTAAATATGTTT+TGG + chr8.1:35458883-35458902 MS.gene008862:intron 15.0%
!!! CAATGTTTTTAATTTTGTAT+TGG + chr8.1:35460267-35460286 MS.gene008862:intron 15.0%
!!! TTTTATGTTTCTTTTCATTA+TGG + chr8.1:35458731-35458750 MS.gene008862:intron 15.0%
!!! TTTTCACATTCATTAAATAA+CGG - chr8.1:35460836-35460855 None:intergenic 15.0%
!!! TTTTTTTGAAGGAATAAATT+TGG + chr8.1:35460109-35460128 MS.gene008862:intron 15.0%
!! AAAAACATTGATGAAAATGA+AGG - chr8.1:35460257-35460276 None:intergenic 20.0%
!! AAAAAGAAAAAGAAGACATT+TGG - chr8.1:35459232-35459251 None:intergenic 20.0%
!! AAAACATTGATGAAAATGAA+GGG - chr8.1:35460256-35460275 None:intergenic 20.0%
!! AAAACTAAGAAAAATACCAA+TGG - chr8.1:35458345-35458364 None:intergenic 20.0%
!! AACTTAACATAGATAGATAA+TGG + chr8.1:35458799-35458818 MS.gene008862:intron 20.0%
!! ACGAAAATTCTTAAATTGAT+TGG + chr8.1:35460186-35460205 MS.gene008862:intron 20.0%
!! AGTAAAATACGCAAAATATT+AGG - chr8.1:35461727-35461746 None:intergenic 20.0%
!! ATTCTTAAATTGATTGGATT+TGG + chr8.1:35460192-35460211 MS.gene008862:intron 20.0%
!! GTAAAATACGCAAAATATTA+GGG - chr8.1:35461726-35461745 None:intergenic 20.0%
!! GTATATTATAACAATTTGGA+CGG - chr8.1:35462401-35462420 None:intergenic 20.0%
!! GTGAAAAAAAGAAACAAAAA+AGG - chr8.1:35461552-35461571 None:intergenic 20.0%
!! GTTAAATAAGAGAAAAATGA+AGG - chr8.1:35461699-35461718 None:intergenic 20.0%
!! TCATTAAGCTAATATCAAAT+TGG - chr8.1:35460630-35460649 None:intergenic 20.0%
!!! AAATCAATGGTTAGTTAATT+TGG - chr8.1:35461120-35461139 None:intergenic 20.0%
!!! AAATGGTCAAATTTCTTTTA+CGG - chr8.1:35458970-35458989 None:intergenic 20.0%
!!! AATAATCTCATTGACTAAAT+TGG - chr8.1:35461027-35461046 None:intergenic 20.0%
!!! AGTTTAAGTAATTTTTATGC+AGG + chr8.1:35460302-35460321 MS.gene008862:intron 20.0%
!!! ATAAAGTACTAACAACAAAT+TGG + chr8.1:35459638-35459657 MS.gene008862:intron 20.0%
!!! ATCTATGTTAAGTTGTTTTT+TGG - chr8.1:35458793-35458812 None:intergenic 20.0%
!!! TATATGGTAGTATTTTTTGA+GGG + chr8.1:35458834-35458853 MS.gene008862:intron 20.0%
!!! TCTATGTTAAGTTGTTTTTT+GGG - chr8.1:35458792-35458811 None:intergenic 20.0%
!!! TTTTGGCTCATATAAAATTA+CGG + chr8.1:35458900-35458919 MS.gene008862:intron 20.0%
! AAACAAGGAGAAGAAAATAA+AGG - chr8.1:35458007-35458026 None:intergenic 25.0%
! AAATTACCCATAATCTTCTA+AGG - chr8.1:35460690-35460709 None:intergenic 25.0%
! AAGGAATAAATTTGGAAAGA+AGG + chr8.1:35460117-35460136 MS.gene008862:intron 25.0%
! AAGTAAATTGATTGAAGCAA+GGG + chr8.1:35460380-35460399 MS.gene008862:intron 25.0%
! AGGAATAAATTTGGAAAGAA+GGG + chr8.1:35460118-35460137 MS.gene008862:intron 25.0%
! AGTAGTAAAGTGAGTAAATA+TGG - chr8.1:35461434-35461453 None:intergenic 25.0%
! AGTCAAAAAGAAGAAAACAA+AGG - chr8.1:35461763-35461782 None:intergenic 25.0%
! ATACAAGAAGGAACTAATAA+TGG + chr8.1:35462417-35462436 MS.gene008862:CDS 25.0%
! ATGGAAATTGGTAAAGTATA+TGG + chr8.1:35458818-35458837 MS.gene008862:intron 25.0%
! ATTGAAAATAAGTACTCCAT+CGG - chr8.1:35460884-35460903 None:intergenic 25.0%
! ATTTACTCACTTTACTACTT+TGG + chr8.1:35461435-35461454 MS.gene008862:intron 25.0%
! GAAAATTTAAGCTGAATATG+TGG + chr8.1:35461179-35461198 MS.gene008862:intron 25.0%
! GTTCAAATGTTAGTGAATTT+AGG + chr8.1:35461406-35461425 MS.gene008862:intron 25.0%
! TAAGTAAATTGATTGAAGCA+AGG + chr8.1:35460379-35460398 MS.gene008862:intron 25.0%
! TACAAGAAGGAACTAATAAT+GGG + chr8.1:35462418-35462437 MS.gene008862:CDS 25.0%
! TCACTTCTCAGTTTATTAAT+AGG + chr8.1:35459523-35459542 MS.gene008862:intron 25.0%
! TGACCACTTTAAATTGAAAA+AGG - chr8.1:35459486-35459505 None:intergenic 25.0%
! TTAGAAGATTATGGGTAATT+TGG + chr8.1:35460689-35460708 MS.gene008862:intron 25.0%
!! CCTTCTTTTATTCTTCTAAT+CGG - chr8.1:35458557-35458576 None:intergenic 25.0%
!! CTTTTCATTATGGTAAAGTA+TGG + chr8.1:35458741-35458760 MS.gene008862:intron 25.0%
!! GAAAGAGATAAAAAGCATAA+GGG - chr8.1:35461498-35461517 None:intergenic 25.0%
!! TGAAAGAGATAAAAAGCATA+AGG - chr8.1:35461499-35461518 None:intergenic 25.0%
!! TTGATATTAGCTTAATGATC+TGG + chr8.1:35460632-35460651 MS.gene008862:CDS 25.0%
!!! AGTCCTTTTTCAATTTAAAG+TGG + chr8.1:35459480-35459499 MS.gene008862:intron 25.0%
!!! AGTTTTTTTTCTCTATTGAG+CGG + chr8.1:35458359-35458378 MS.gene008862:intron 25.0%
!!! ATATGGTAGTATTTTTTGAG+GGG + chr8.1:35458835-35458854 MS.gene008862:intron 25.0%
!!! CATAGATAGATAATGGAAAT+TGG + chr8.1:35458806-35458825 MS.gene008862:intron 25.0%
!!! GATTTGGTTTTGCATTAAAT+AGG + chr8.1:35460208-35460227 MS.gene008862:intron 25.0%
!!! GTATATGGTAGTATTTTTTG+AGG + chr8.1:35458833-35458852 MS.gene008862:intron 25.0%
!!! TTGGTAGAAAAGTTTGATTT+TGG - chr8.1:35458067-35458086 None:intergenic 25.0%
AAAAGCTAGCATAAATGTGT+GGG + chr8.1:35459722-35459741 MS.gene008862:CDS 30.0%
AAAATTGAAACGTGTGAGAA+TGG + chr8.1:35458616-35458635 MS.gene008862:CDS 30.0%
AAAGTGACCTTAGAAGATTA+TGG + chr8.1:35460680-35460699 MS.gene008862:intron 30.0%
AACTTGTGAAACTCTTCATT+TGG - chr8.1:35458086-35458105 None:intergenic 30.0%
AAGTGACCTTAGAAGATTAT+GGG + chr8.1:35460681-35460700 MS.gene008862:intron 30.0%
AATTCAGCTTGTCTCAAATA+TGG + chr8.1:35459769-35459788 MS.gene008862:CDS 30.0%
ACTAGCAGTAATATTGCAAT+TGG + chr8.1:35460568-35460587 MS.gene008862:CDS 30.0%
AGTAAATACCAGTAGAAACT+TGG - chr8.1:35460726-35460745 None:intergenic 30.0%
ATAGTGAATACAATGTCAAC+AGG + chr8.1:35462237-35462256 MS.gene008862:CDS 30.0%
ATCCTTTAAGTGTGTGAATA+GGG - chr8.1:35462187-35462206 None:intergenic 30.0%
ATGAAGAGTTTCACAAGTTT+AGG + chr8.1:35458087-35458106 MS.gene008862:CDS 30.0%
CAACATAAATGACACACAAA+AGG + chr8.1:35461350-35461369 MS.gene008862:intron 30.0%
CCGATTAGAAGAATAAAAGA+AGG + chr8.1:35458554-35458573 MS.gene008862:CDS 30.0%
CTTCTCAGTTTATTAATAGG+TGG + chr8.1:35459526-35459545 MS.gene008862:intron 30.0%
CTTTAAGTCGTAAAAAGCAA+AGG - chr8.1:35461639-35461658 None:intergenic 30.0%
GTATCGTTGAAGATTTACTA+GGG + chr8.1:35461870-35461889 MS.gene008862:intron 30.0%
GTTTATTCTTCAGAAACAAG+TGG + chr8.1:35458041-35458060 MS.gene008862:CDS 30.0%
TAATGAGACTTCCAAACTAA+TGG - chr8.1:35461233-35461252 None:intergenic 30.0%
TAGAATTGTAGAAGAAGCAA+GGG + chr8.1:35461278-35461297 MS.gene008862:intron 30.0%
TAGATTCTTCACTTATTGGA+CGG + chr8.1:35460356-35460375 MS.gene008862:CDS 30.0%
TAGTGAATACAATGTCAACA+GGG + chr8.1:35462238-35462257 MS.gene008862:CDS 30.0%
TATCCTTTAAGTGTGTGAAT+AGG - chr8.1:35462188-35462207 None:intergenic 30.0%
TCCAATAAGTGAAGAATCTA+GGG - chr8.1:35460356-35460375 None:intergenic 30.0%
TCTCCAACTTCTACATATAA+TGG + chr8.1:35460601-35460620 MS.gene008862:CDS 30.0%
TGAAGGAATAAAAAAAGGTG+TGG - chr8.1:35461682-35461701 None:intergenic 30.0%
TGTATCGTTGAAGATTTACT+AGG + chr8.1:35461869-35461888 MS.gene008862:intron 30.0%
TGTATTAATGTGAGACTACA+AGG - chr8.1:35459350-35459369 None:intergenic 30.0%
TTAGCTACAACATTGAGATT+TGG - chr8.1:35462378-35462397 None:intergenic 30.0%
TTGAAAATAAGTACTCCATC+GGG - chr8.1:35460883-35460902 None:intergenic 30.0%
TTGATTATAAATGTGATCGG+AGG - chr8.1:35460752-35460771 None:intergenic 30.0%
! AGACTAACTTTTCTTTTGGA+AGG - chr8.1:35458947-35458966 None:intergenic 30.0%
! CTTTAGAAATATGCAAGTTG+TGG + chr8.1:35462326-35462345 MS.gene008862:CDS 30.0%
! TATTAGCTTAATGATCTGGA+AGG + chr8.1:35460636-35460655 MS.gene008862:CDS 30.0%
!! ATAATCTTCTAAGGTCACTT+TGG - chr8.1:35460681-35460700 None:intergenic 30.0%
!! GGAAATTACTTTTACTATGG+TGG + chr8.1:35462444-35462463 MS.gene008862:CDS 30.0%
!! TGGGGAAATTACTTTTACTA+TGG + chr8.1:35462441-35462460 MS.gene008862:CDS 30.0%
!! TTGTTCGGTTTTATGTGTAT+AGG + chr8.1:35458430-35458449 MS.gene008862:intron 30.0%
!! TTTTCTTCTCCTTGTTTCTT+TGG + chr8.1:35458010-35458029 MS.gene008862:CDS 30.0%
!!! TTGCACTGCTTTTTTTTAGT+AGG + chr8.1:35459908-35459927 MS.gene008862:intron 30.0%
AAAATGTCCACTACCCATTT+GGG - chr8.1:35462277-35462296 None:intergenic 35.0%
AAAGTGGTCACTGAATTTCA+TGG + chr8.1:35459496-35459515 MS.gene008862:intron 35.0%
AAATACCAGTAGAAACTTGG+AGG - chr8.1:35460723-35460742 None:intergenic 35.0%
AAGAGTTTCACAAGTTTAGG+AGG + chr8.1:35458090-35458109 MS.gene008862:CDS 35.0%
AATACCAGTAGAAACTTGGA+GGG - chr8.1:35460722-35460741 None:intergenic 35.0%
ACAACATTGAGATTTGGCAA+GGG - chr8.1:35462372-35462391 None:intergenic 35.0%
ACATACTTTAGTCCCTATGT+AGG - chr8.1:35459032-35459051 None:intergenic 35.0%
ACCCTAGATTCTTCACTTAT+TGG + chr8.1:35460352-35460371 MS.gene008862:CDS 35.0%
ACCGACAAGTAAAAAATGAG+TGG - chr8.1:35459939-35459958 None:intergenic 35.0%
AGTGCAACAATGGTTCAATT+TGG + chr8.1:35462207-35462226 MS.gene008862:CDS 35.0%
ATCCCTATTCACACACTTAA+AGG + chr8.1:35462182-35462201 MS.gene008862:CDS 35.0%
ATGGATCTGGAAACTTGATT+GGG + chr8.1:35462148-35462167 MS.gene008862:CDS 35.0%
ATTAATGTGAGACTACAAGG+AGG - chr8.1:35459347-35459366 None:intergenic 35.0%
CAAAAGCTAGCATAAATGTG+TGG + chr8.1:35459721-35459740 MS.gene008862:CDS 35.0%
CAAGGAGAAGAAAATAAAGG+AGG - chr8.1:35458004-35458023 None:intergenic 35.0%
CACAATGTTAGTGTTGTGTA+TGG + chr8.1:35462013-35462032 MS.gene008862:intron 35.0%
CATGACAAACCAAAGAAACA+AGG - chr8.1:35458022-35458041 None:intergenic 35.0%
CCAACTTCTACATATAATGG+AGG + chr8.1:35460604-35460623 MS.gene008862:CDS 35.0%
CCATAAAAATGCATCTTCGA+CGG + chr8.1:35458114-35458133 MS.gene008862:CDS 35.0%
CCGACAAGTAAAAAATGAGT+GGG - chr8.1:35459938-35459957 None:intergenic 35.0%
CCTCCATTATATGTAGAAGT+TGG - chr8.1:35460607-35460626 None:intergenic 35.0%
CTTAAAGGATAGTGCAACAA+TGG + chr8.1:35462197-35462216 MS.gene008862:CDS 35.0%
GAGAAGAAAATAAAGGAGGA+GGG - chr8.1:35458000-35458019 None:intergenic 35.0%
GCTTGCTCTATTAACACATT+TGG + chr8.1:35458590-35458609 MS.gene008862:CDS 35.0%
GTAGAATTGTAGAAGAAGCA+AGG + chr8.1:35461277-35461296 MS.gene008862:intron 35.0%
GTCCAATAAGTGAAGAATCT+AGG - chr8.1:35460357-35460376 None:intergenic 35.0%
TAAATTGATTGAAGCAAGGG+AGG + chr8.1:35460383-35460402 MS.gene008862:intron 35.0%
TACAACATTGAGATTTGGCA+AGG - chr8.1:35462373-35462392 None:intergenic 35.0%
TATGGATCTGGAAACTTGAT+TGG + chr8.1:35462147-35462166 MS.gene008862:CDS 35.0%
TCTCAAATATGGATCATCTC+TGG + chr8.1:35459780-35459799 MS.gene008862:CDS 35.0%
TGAGACAAGCTGAATTCATA+TGG - chr8.1:35459765-35459784 None:intergenic 35.0%
TGATGAAAATGAAGGGTAGT+TGG - chr8.1:35460249-35460268 None:intergenic 35.0%
TGCTACAATTTACTTTGCTC+TGG + chr8.1:35460535-35460554 MS.gene008862:CDS 35.0%
TGGCTCATATAAAATTACGG+TGG + chr8.1:35458903-35458922 MS.gene008862:intron 35.0%
TTGCTTCTATTTATGCGTGT+AGG + chr8.1:35462095-35462114 MS.gene008862:intron 35.0%
TTTATGTGTATAGGATCCTC+CGG + chr8.1:35458439-35458458 MS.gene008862:intron 35.0%
TTTCAATTGTGGAGTTTCTC+AGG + chr8.1:35458506-35458525 MS.gene008862:CDS 35.0%
! AAGAAGGAACTAATAATGGG+TGG + chr8.1:35462421-35462440 MS.gene008862:CDS 35.0%
! ACGGAGACTAACTTTTCTTT+TGG - chr8.1:35458951-35458970 None:intergenic 35.0%
!! AGAAGAAAACAAAGGTACCA+AGG - chr8.1:35461755-35461774 None:intergenic 35.0%
!! AGAAGGAACTAATAATGGGT+GGG + chr8.1:35462422-35462441 MS.gene008862:CDS 35.0%
!!! CCCACTCATTTTTTACTTGT+CGG + chr8.1:35459935-35459954 MS.gene008862:intron 35.0%
!!! GTGATTTTAGCTCCTACATA+GGG + chr8.1:35459017-35459036 MS.gene008862:intron 35.0%
!!! GTTTCTGTTGTTTTCTGTGA+TGG - chr8.1:35458673-35458692 None:intergenic 35.0%
!!! TGTGATTTTAGCTCCTACAT+AGG + chr8.1:35459016-35459035 MS.gene008862:intron 35.0%
!!! TTTTTTGAGGGGAAAGCATA+TGG + chr8.1:35458846-35458865 MS.gene008862:intron 35.0%
AACATAGGATGGTCAAAAGC+TGG - chr8.1:35458309-35458328 None:intergenic 40.0%
AAGTGTGTGAATAGGGATGA+TGG - chr8.1:35462180-35462199 None:intergenic 40.0%
AATATGTGGTCAATGCATGG+TGG + chr8.1:35461193-35461212 MS.gene008862:intron 40.0%
ACATATGCTTGTGTTCGATG+TGG + chr8.1:35461933-35461952 MS.gene008862:intron 40.0%
ACCTGAATTGTCTTAACAGC+AGG - chr8.1:35458149-35458168 None:intergenic 40.0%
ACTGATGCATATCAAGGAAC+TGG + chr8.1:35460511-35460530 MS.gene008862:CDS 40.0%
ATATCCCTCCAAGTTTCTAC+TGG + chr8.1:35460715-35460734 MS.gene008862:intron 40.0%
ATATGTGGTCAATGCATGGT+GGG + chr8.1:35461194-35461213 MS.gene008862:intron 40.0%
ATGCAGTTGGATATGTGAGT+GGG + chr8.1:35459682-35459701 MS.gene008862:CDS 40.0%
CAAAATGTCCACTACCCATT+TGG - chr8.1:35462278-35462297 None:intergenic 40.0%
CAGTCTATGATATCACCATC+AGG - chr8.1:35458267-35458286 None:intergenic 40.0%
CATATGGACTTTCCACTTGT+GGG - chr8.1:35459749-35459768 None:intergenic 40.0%
CTAATCGGAATTGTTCCTTC+TGG - chr8.1:35458542-35458561 None:intergenic 40.0%
CTGAATATGTGGTCAATGCA+TGG + chr8.1:35461190-35461209 MS.gene008862:intron 40.0%
CTGATGCATATCAAGGAACT+GGG + chr8.1:35460512-35460531 MS.gene008862:CDS 40.0%
CTGGAAACTTGATTGGGTAT+TGG + chr8.1:35462154-35462173 MS.gene008862:CDS 40.0%
GATCTCAATACCATTGAAGC+TGG + chr8.1:35459816-35459835 MS.gene008862:CDS 40.0%
GCAACAATGGTTCAATTTGG+TGG + chr8.1:35462210-35462229 MS.gene008862:CDS 40.0%
GGAGAAGAAAATAAAGGAGG+AGG - chr8.1:35458001-35458020 None:intergenic 40.0%
GTTGAGAGGACAAAAACCAT+TGG + chr8.1:35458326-35458345 MS.gene008862:CDS 40.0%
TCAATACCATTGAAGCTGGT+TGG + chr8.1:35459820-35459839 MS.gene008862:CDS 40.0%
TCATATGGACTTTCCACTTG+TGG - chr8.1:35459750-35459769 None:intergenic 40.0%
TCTCACACTTCTACCCAAAT+GGG + chr8.1:35462261-35462280 MS.gene008862:CDS 40.0%
TCTCGTAGTCAAAGTCTCAA+AGG + chr8.1:35461157-35461176 MS.gene008862:intron 40.0%
TCTGACACACATTCAATCTG+AGG - chr8.1:35461907-35461926 None:intergenic 40.0%
TTGCAGACTGATGCATATCA+AGG + chr8.1:35460505-35460524 MS.gene008862:intron 40.0%
TTTGACCATCCTATGTTGAG+AGG + chr8.1:35458312-35458331 MS.gene008862:CDS 40.0%
TTTGTCCTCTCAACATAGGA+TGG - chr8.1:35458320-35458339 None:intergenic 40.0%
! ACCAAGAGAGCATAAGCAAA+TGG + chr8.1:35458466-35458485 MS.gene008862:CDS 40.0%
! CCAAGAGAGCATAAGCAAAT+GGG + chr8.1:35458467-35458486 MS.gene008862:CDS 40.0%
! CCGTCGAAGATGCATTTTTA+TGG - chr8.1:35458117-35458136 None:intergenic 40.0%
! CTCATTGACTAAATTGGCAC+TGG - chr8.1:35461021-35461040 None:intergenic 40.0%
! GGACATTTTACATTCCTTCC+AGG - chr8.1:35462471-35462490 None:intergenic 40.0%
! GGTTTTTGTCCTCTCAACAT+AGG - chr8.1:35458324-35458343 None:intergenic 40.0%
! TACTTTTACTATGGTGGTCC+TGG + chr8.1:35462450-35462469 MS.gene008862:CDS 40.0%
! TGGATCATCTCTGGTTCATT+TGG + chr8.1:35459789-35459808 MS.gene008862:CDS 40.0%
!! AGAAAACAAAGGTACCAAGG+TGG - chr8.1:35461752-35461771 None:intergenic 40.0%
!! GAAGGAACTAATAATGGGTG+GGG + chr8.1:35462423-35462442 MS.gene008862:CDS 40.0%
AATGATGCACAGCATGCAGT+TGG + chr8.1:35459669-35459688 MS.gene008862:intron 45.0%
ACTTCTACCCAAATGGGTAG+TGG + chr8.1:35462267-35462286 MS.gene008862:CDS 45.0%
AGGATCCAAAGCATGGGAAT+TGG + chr8.1:35462115-35462134 MS.gene008862:intron 45.0%
AGGTGTGGCGTTCAGAATTA+TGG - chr8.1:35461667-35461686 None:intergenic 45.0%
ATATCACCATCAGGACTCTG+CGG - chr8.1:35458258-35458277 None:intergenic 45.0%
ATCCAAAGCATGGGAATTGG+TGG + chr8.1:35462118-35462137 MS.gene008862:CDS 45.0%
ATTGATTGAAGCAAGGGAGG+TGG + chr8.1:35460386-35460405 MS.gene008862:intron 45.0%
CATGCAGTTGGATATGTGAG+TGG + chr8.1:35459681-35459700 MS.gene008862:CDS 45.0%
CCCATTTGCTTATGCTCTCT+TGG - chr8.1:35458470-35458489 None:intergenic 45.0%
GCCTGCTGTTAAGACAATTC+AGG + chr8.1:35458145-35458164 MS.gene008862:CDS 45.0%
GTCTCACACTTCTACCCAAA+TGG + chr8.1:35462260-35462279 MS.gene008862:CDS 45.0%
GTGAGTGGGAATGAGTACTA+TGG + chr8.1:35459696-35459715 MS.gene008862:CDS 45.0%
TATGTGGTCAATGCATGGTG+GGG + chr8.1:35461195-35461214 MS.gene008862:intron 45.0%
TCAGGTGAATCATGTCCAGA+AGG + chr8.1:35458524-35458543 MS.gene008862:CDS 45.0%
TGGTGGCTTGAATATGGATC+TGG + chr8.1:35462135-35462154 MS.gene008862:CDS 45.0%
TGTGAGACTACAAGGAGGAT+TGG - chr8.1:35459342-35459361 None:intergenic 45.0%
TGTTTATAAACGTGGCCCGA+TGG + chr8.1:35460865-35460884 MS.gene008862:intron 45.0%
TTATGCTCTCTTGGTCTCTC+CGG - chr8.1:35458461-35458480 None:intergenic 45.0%
TTTACTATGGTGGTCCTGGA+AGG + chr8.1:35462454-35462473 MS.gene008862:CDS 45.0%
! AGACTACAAGGAGGATTGGT+TGG - chr8.1:35459338-35459357 None:intergenic 45.0%
! AGTGGACATTTTGCTGAGGA+GGG + chr8.1:35462285-35462304 MS.gene008862:CDS 45.0%
! GCGTATTTTACTCACCACCT+TGG + chr8.1:35461735-35461754 MS.gene008862:intron 45.0%
! GGGAATTGGTGGCTTGAATA+TGG + chr8.1:35462129-35462148 MS.gene008862:CDS 45.0%
!! TAGTGGACATTTTGCTGAGG+AGG + chr8.1:35462284-35462303 MS.gene008862:CDS 45.0%
!!! TAAATACATTTTTTATTAAT+GGG - chr8.1:35460460-35460479 None:intergenic 5.0%
!!! TTAAATACATTTTTTATTAA+TGG - chr8.1:35460461-35460480 None:intergenic 5.0%
AAAGGAGGAGGGAGATGACT+GGG - chr8.1:35457989-35458008 None:intergenic 50.0%
AAATGTGTGGGTCCCACAAG+TGG + chr8.1:35459734-35459753 MS.gene008862:CDS 50.0%
AGCCACCAATTCCCATGCTT+TGG - chr8.1:35462123-35462142 None:intergenic 50.0%
AGCTATCCCCATAGAGCTCT+GGG - chr8.1:35460332-35460351 None:intergenic 50.0%
ATGTGGTCAATGCATGGTGG+GGG + chr8.1:35461196-35461215 MS.gene008862:intron 50.0%
GCGTGTAGGATCCAAAGCAT+GGG + chr8.1:35462109-35462128 MS.gene008862:intron 50.0%
TAAAGGAGGAGGGAGATGAC+TGG - chr8.1:35457990-35458009 None:intergenic 50.0%
TACCATTGAAGCTGGTTGGC+AGG + chr8.1:35459824-35459843 MS.gene008862:CDS 50.0%
TAGCTATCCCCATAGAGCTC+TGG - chr8.1:35460333-35460352 None:intergenic 50.0%
TGCGTGTAGGATCCAAAGCA+TGG + chr8.1:35462108-35462127 MS.gene008862:intron 50.0%
TTGTACCTCATGTCCATCGC+CGG - chr8.1:35458648-35458667 None:intergenic 50.0%
! CATGGGGCACTCCATTAGTT+TGG + chr8.1:35461219-35461238 MS.gene008862:intron 50.0%
! GTTTTTCCGCAGAGTCCTGA+TGG + chr8.1:35458249-35458268 MS.gene008862:intron 50.0%
!!! GGGTAGTGGACATTTTGCTG+AGG + chr8.1:35462281-35462300 MS.gene008862:CDS 50.0%
AAGGAGGAGGGAGATGACTG+GGG - chr8.1:35457988-35458007 None:intergenic 55.0%
AGGTCAGCCCAGAGCTCTAT+GGG + chr8.1:35460322-35460341 MS.gene008862:intron 55.0%
CGTGTGAGAATGGACACCAC+CGG + chr8.1:35458626-35458645 MS.gene008862:CDS 55.0%
TGCCTGCCAACCAGCTTCAA+TGG - chr8.1:35459829-35459848 None:intergenic 55.0%
! TCAATGCATGGTGGGGGCAT+GGG + chr8.1:35461202-35461221 MS.gene008862:intron 55.0%
! TTTTGTGTGCGCGCGCGTTT+TGG + chr8.1:35462046-35462065 MS.gene008862:intron 55.0%
AGAATGGACACCACCGGCGA+TGG + chr8.1:35458632-35458651 MS.gene008862:CDS 60.0%
CAGGTCAGCCCAGAGCTCTA+TGG + chr8.1:35460321-35460340 MS.gene008862:intron 60.0%
GGTCAGCCCAGAGCTCTATG+GGG + chr8.1:35460323-35460342 MS.gene008862:intron 60.0%
TACCTCATGTCCATCGCCGG+TGG - chr8.1:35458645-35458664 None:intergenic 60.0%
TGCTCTCTTGGTCTCTCCGG+AGG - chr8.1:35458458-35458477 None:intergenic 60.0%
! CAATGCATGGTGGGGGCATG+GGG + chr8.1:35461203-35461222 MS.gene008862:intron 60.0%
! GTCAATGCATGGTGGGGGCA+TGG + chr8.1:35461201-35461220 MS.gene008862:intron 60.0%
CACCACCGGCGATGGACATG+AGG + chr8.1:35458640-35458659 MS.gene008862:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 35457972 35462494 35457972 ID=MS.gene008862
chr8.1 mRNA 35457972 35462494 35457972 ID=MS.gene008862.t1;Parent=MS.gene008862
chr8.1 exon 35457972 35458166 35457972 ID=MS.gene008862.t1.exon1;Parent=MS.gene008862.t1
chr8.1 CDS 35457972 35458166 35457972 ID=cds.MS.gene008862.t1;Parent=MS.gene008862.t1
chr8.1 exon 35458261 35458347 35458261 ID=MS.gene008862.t1.exon2;Parent=MS.gene008862.t1
chr8.1 CDS 35458261 35458347 35458261 ID=cds.MS.gene008862.t1;Parent=MS.gene008862.t1
chr8.1 exon 35458452 35458661 35458452 ID=MS.gene008862.t1.exon3;Parent=MS.gene008862.t1
chr8.1 CDS 35458452 35458661 35458452 ID=cds.MS.gene008862.t1;Parent=MS.gene008862.t1
chr8.1 exon 35459681 35459845 35459681 ID=MS.gene008862.t1.exon4;Parent=MS.gene008862.t1
chr8.1 CDS 35459681 35459845 35459681 ID=cds.MS.gene008862.t1;Parent=MS.gene008862.t1
chr8.1 exon 35460324 35460377 35460324 ID=MS.gene008862.t1.exon5;Parent=MS.gene008862.t1
chr8.1 CDS 35460324 35460377 35460324 ID=cds.MS.gene008862.t1;Parent=MS.gene008862.t1
chr8.1 exon 35460511 35460657 35460511 ID=MS.gene008862.t1.exon6;Parent=MS.gene008862.t1
chr8.1 CDS 35460511 35460657 35460511 ID=cds.MS.gene008862.t1;Parent=MS.gene008862.t1
chr8.1 exon 35462117 35462494 35462117 ID=MS.gene008862.t1.exon7;Parent=MS.gene008862.t1
chr8.1 CDS 35462117 35462494 35462117 ID=cds.MS.gene008862.t1;Parent=MS.gene008862.t1
Gene Sequence

>MS.gene008862

ATGGATTTTAGCTCCCCAGTCATCTCCCTCCTCCTTTATTTTCTTCTCCTTGTTTCTTTGGTTTGTCATGTTTATTCTTCAGAAACAAGTGGCCAAAATCAAACTTTTCTACCAAATGAAGAGTTTCACAAGTTTAGGAGGACCATAAAAATGCATCTTCGACGGATCAACAAGCCTGCTGTTAAGACAATTCAGGTATGTTAAAAATGTCTTCAAAAAATTTCTGAATTTTTTTGAAGAAAAAATAAATGGTGTTTGTGTGATATGTGTTTGTGTTGTTTTTCCGCAGAGTCCTGATGGTGATATCATAGACTGTGTTATGTCTCATAGACAACCAGCTTTTGACCATCCTATGTTGAGAGGACAAAAACCATTGGTATTTTTCTTAGTTTTTTTTCTCTATTGAGCGGAAATTAAAATAAAAAATTTCTTTTATATAATTATATTTCAACTTATAATTGTTCGGTTTTATGTGTATAGGATCCTCCGGAGAGACCAAGAGAGCATAAGCAAATGGGCATTGAAAATTATAACTTTCAATTGTGGAGTTTCTCAGGTGAATCATGTCCAGAAGGAACAATTCCGATTAGAAGAATAAAAGAAGGTGACATGTTAAGAGCTTGCTCTATTAACACATTTGGAAGAAAATTGAAACGTGTGAGAATGGACACCACCGGCGATGGACATGAGGTACAATGCCATCACAGAAAACAACAGAAACTCAGATTATTTTGTCTCATAATTTCTTTTATATTATATTTTTATGTTTCTTTTCATTATGGTAAAGTATGGAATAAATAAACAACTTAAGAGATAACCCAAAAAACAACTTAACATAGATAGATAATGGAAATTGGTAAAGTATATGGTAGTATTTTTTGAGGGGAAAGCATATGGTAGTTGAGTATTTAATTTTTCGTTAAATATGTTTTGGCTCATATAAAATTACGGTGGTAAGTTTAGTCTTTATAGCCTTCCAAAAGAAAAGTTAGTCTCCGTAAAAGAAATTTGACCATTTTTGTCTATATTTTCTTTATAAGAACTTGTGATTTTAGCTCCTACATAGGGACTAAAGTATGTATTAAATTTTATAAAAAGTAAAGATTAAAAATTGTTCAATAAGTTTTTTTTTTTTGAAACAGTTCAAAAAGAATTTATAAAGACTAAAACAATTTTTTTATATTTTATAAGTTACAAAAACATATGTATCCATTCATTTCTTTTGAAATAATTAATATCGGTCATAAAAGAGAAATTCCAAATGTCTTCTTTTTCTTTTTATATTATATTTTTTTAAAAATTTATAAGTATGAAAATGAAGATAGTACGATTAGCACAAAAAAATTGAAAATGATCTGTAGTACCAACCAATCCTCCTTGTAGTCTCACATTAATACAAACAAAATAATATTATGATAACTTCTTCCAAAAATAATATTCTGATAAAAGCATAATAGACAAGTGTTCTTTTTTTTTTTTTTTGTCTTTCTAATTTACTCATGAAGCTAAGTCCTTTTTCAATTTAAAGTGGTCACTGAATTTCATGGTTACTCACTTCTCAGTTTATTAATAGGTGGACCAATTATTTTTATCTTCAATAGAAATAATCTTAGACACAACAAAAACCAACATTGAATCTAATATAAATCTTGTGCTATTTTTGAAAATAAAGTACTAACAACAAATTGGTTCTTCTTAATGATGCACAGCATGCAGTTGGATATGTGAGTGGGAATGAGTACTATGGAGCAAAAGCTAGCATAAATGTGTGGGTCCCACAAGTGGAAAGTCCATATGAATTCAGCTTGTCTCAAATATGGATCATCTCTGGTTCATTTGGAGTAGATCTCAATACCATTGAAGCTGGTTGGCAGGCATGTTGATTATTTTCATTAATTACTTCATCTTAGACAATCAATTCATTCAATCACTTCTTTTGCACTGCTTTTTTTTAGTAGGTACTCCCACTCATTTTTTACTTGTCGGTTACGTGTATTTCATACGTCTCATAATAAGTGACATTGTAGACAGTTGCACATAGATATTTTTGACATTTAATATTTTTGATTATGTATTAGTAAAAATTATAAATGCAAATCCAATACTTAATAATGATACTTTAATAAAAATGATTTTTTTTTTTTGAAGGAATAAATTTGGAAAGAAGGGAGTATAGTATTAATCTTTTACACAATTTTTTTAAACTAATTTTACACGAAAATTCTTAAATTGATTGGATTTGGTTTTGCATTAAATAGGCAGTTAGAATGTCAACCAACTACCCTTCATTTTCATCAATGTTTTTAATTTTGTATTGGTCAATCAAGCTAAGTTTAAGTAATTTTTATGCAGGTCAGCCCAGAGCTCTATGGGGATAGCTACCCTAGATTCTTCACTTATTGGACGGTAAGTAAATTGATTGAAGCAAGGGAGGTGGAAAAGTAAAATATCTATGATTGATACTACTTGCTTCAAGTGTAAGATACCCATTAATAAAAAATGTATTTAAAAAAATGATAATGGCTAGTAATAATTGCAGACTGATGCATATCAAGGAACTGGGTGCTACAATTTACTTTGCTCTGGCTTTGTTCAAACTAGCAGTAATATTGCAATTGGAGCAGCAATTTCTCCAACTTCTACATATAATGGAGGCCAATTTGATATTAGCTTAATGATCTGGAAGGTAATTTTCTGCTCAGAATACCAAAGTGACCTTAGAAGATTATGGGTAATTTGGTATATATCCCTCCAAGTTTCTACTGGTATTTACTACTCCTCCGATCACATTTATAATCAATTTTTTTTTTAAATTTATTAAATAACTGATGTATCTAGATTATAATATAGTCTAGATACATCCGTTATTTAATGAATGTGAAAAATTAAAAATTGTTTATAAACGTGGCCCGATGGAGTACTTATTTTCAATAATAATACAGCTTCAAATATGTAAAATAAGTCAATTTAGTTACTAAAGTTAAAACAAAATAAAATAGTTCTTCGAAATAAAAAAAATTCAATTAATTAATTATAATTAATTATTCCAGTGCCAATTTAGTCAATGAGATTATTGCATATTGACAAATGTGATTAGAAACTTTTAGTTTTAAAGAACATCTGAATATTTTTTTAACTTCAATAACCAAATTAACTAACCATTGATTTGTTACAAAATATTGAAGTCTCGTAGTCAAAGTCTCAAAGGAAAATTTAAGCTGAATATGTGGTCAATGCATGGTGGGGGCATGGGGCACTCCATTAGTTTGGAAGTCTCATTATATGCTTTCGAATTTTGTTATTATGTAGAATTGTAGAAGAAGCAAGGGCAAGTTTATTATAACATGTTCACAACATAATAATTTGTTTTGAAAAAAGCAACATAAATGACACACAAAAGGAGAAAAACAATATAATGAACAAAATTTTCTAGTGTTCAAATGTTAGTGAATTTAGGTACCATATTTACTCACTTTACTACTTTGGTACATTACACGCATGCACTGTGAATTTGAATGTAACTCCCTTATGCTTTTTATCTCTTTCAAGATTCTTTTTCTTTATTCTTTTTTCTTTCCCTTTTTTGTTTCTTTTTTTCACCACATAATTTATCATATTTTTTACTTTCTCTCATTCTTTCTGAGCACTTTGAGAACATCATTTTCCTTTGCTTTTTACGACTTAAAGATATTCCATAATTCTGAACGCCACACCTTTTTTTATTCCTTCATTTTTCTCTTATTTAACAAAACCCTAATATTTTGCGTATTTTACTCACCACCTTGGTACCTTTGTTTTCTTCTTTTTGACTCATTTCTATTATCATGTCAGCTAACATGCTGCTCTTAATGATTATGTTTATGTTTGAGTAATTTAACTTTGTCACAAAACAAAAGTTGTATCGTTGAAGATTTACTAGGGTAACATTGATACCTCAGATTGAATGTGTGTCAGATGATTAACATATGCTTGTGTTCGATGTGGTTATATTAATTCAGTTACTTCTATTTTTCTTTTTTAAACTATTAAAAAGGTTTATATCACAATGTTAGTGTTGTGTATGGTATTTTTTTTTTTTGTGTGCGCGCGCGTTTTGGTGTTTGTGTTTCATATATAGTTTACTTTGCTTCTATTTATGCGTGTAGGATCCAAAGCATGGGAATTGGTGGCTTGAATATGGATCTGGAAACTTGATTGGGTATTGGCCATCATCCCTATTCACACACTTAAAGGATAGTGCAACAATGGTTCAATTTGGTGGAGAAATAGTGAATACAATGTCAACAGGGTCTCACACTTCTACCCAAATGGGTAGTGGACATTTTGCTGAGGAGGGTTTTGCAAAAGCTGCATACTTTAGAAATATGCAAGTTGTGGATTCTGATAACAGCTTGATTCCCTTGCCAAATCTCAATGTTGTAGCTAACCGTCCAAATTGTTATAATATACAAGAAGGAACTAATAATGGGTGGGGAAATTACTTTTACTATGGTGGTCCTGGAAGGAATGTAAAATGTCCCTAA
Protein sequence

>MS.gene008862.t1

MDFSSPVISLLLYFLLLVSLVCHVYSSETSGQNQTFLPNEEFHKFRRTIKMHLRRINKPAVKTIQSPDGDIIDCVMSHRQPAFDHPMLRGQKPLDPPERPREHKQMGIENYNFQLWSFSGESCPEGTIPIRRIKEGDMLRACSINTFGRKLKRVRMDTTGDGHEHAVGYVSGNEYYGAKASINVWVPQVESPYEFSLSQIWIISGSFGVDLNTIEAGWQVSPELYGDSYPRFFTYWTTDAYQGTGCYNLLCSGFVQTSSNIAIGAAISPTSTYNGGQFDISLMIWKDPKHGNWWLEYGSGNLIGYWPSSLFTHLKDSATMVQFGGEIVNTMSTGSHTSTQMGSGHFAEEGFAKAAYFRNMQVVDSDNSLIPLPNLNVVANRPNCYNIQEGTNNGWGNYFYYGGPGRNVKCP