AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene011443

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene011443.t1 MTR_5g035070 65.808 427 128 6 7 420 23 444 0.0 541
MS.gene011443.t1 MTR_5g035090 59.712 417 154 7 10 417 24 435 5.29e-179 506
MS.gene011443.t1 MTR_5g035150 57.946 409 164 4 10 413 30 435 1.66e-168 479
MS.gene011443.t1 MTR_3g109840 56.250 416 162 9 5 416 23 422 3.66e-159 455
MS.gene011443.t1 MTR_5g035080 60.955 356 129 4 63 413 74 424 3.32e-157 451
MS.gene011443.t1 MTR_8g104970 53.786 383 167 5 43 420 99 476 3.84e-146 424
MS.gene011443.t1 MTR_7g033230 55.432 359 146 7 64 415 264 615 1.02e-138 410
MS.gene011443.t1 MTR_7g033230 55.432 359 146 7 64 415 334 685 9.80e-138 410
MS.gene011443.t1 MTR_5g035140 57.088 261 103 4 156 411 11 267 3.12e-101 302
MS.gene011443.t1 MTR_6g016880 37.237 427 236 14 1 410 9 420 5.89e-92 284
MS.gene011443.t1 MTR_8g099300 41.026 351 197 8 64 409 74 419 1.32e-90 280
MS.gene011443.t1 MTR_7g019120 37.306 386 215 10 30 410 58 421 4.67e-88 274
MS.gene011443.t1 MTR_8g027900 39.660 353 204 6 61 409 208 555 3.26e-87 276
MS.gene011443.t1 MTR_6g016875 40.503 358 198 8 60 410 60 409 4.24e-84 264
MS.gene011443.t1 MTR_6g007777 37.634 372 208 9 47 411 105 459 1.76e-80 256
MS.gene011443.t1 MTR_2g016060 38.187 364 183 12 69 411 60 402 1.73e-79 251
MS.gene011443.t1 MTR_1g076690 36.011 361 207 11 64 411 223 572 2.78e-67 224
MS.gene011443.t1 MTR_6g061710 35.083 362 209 12 64 411 167 516 3.91e-63 212
MS.gene011443.t1 MTR_7g094750 31.868 364 212 14 64 411 91 434 5.14e-49 172
MS.gene011443.t1 MTR_0136s0010 33.973 365 193 17 62 405 168 505 2.06e-47 170
MS.gene011443.t1 MTR_7g093450 32.242 397 211 14 35 405 219 583 8.17e-46 167
MS.gene011443.t1 MTR_5g059400 32.095 377 207 16 64 416 175 526 1.83e-45 165
MS.gene011443.t1 MTR_1g116130 32.095 377 207 16 64 416 175 526 1.83e-45 165
MS.gene011443.t1 MTR_4g126950 30.645 372 198 18 64 409 109 446 8.93e-44 159
MS.gene011443.t1 MTR_1g058380 30.120 415 234 15 13 405 10 390 6.23e-43 155
MS.gene011443.t1 MTR_4g121560 31.063 367 205 15 64 409 114 453 8.00e-43 156
MS.gene011443.t1 MTR_5g071350 30.000 380 212 11 56 405 40 395 4.23e-42 153
MS.gene011443.t1 MTR_5g035120 52.448 143 66 2 13 154 23 164 1.91e-41 145
MS.gene011443.t1 MTR_2g022560 31.781 365 214 14 64 413 97 441 5.07e-41 151
MS.gene011443.t1 MTR_7g109550 32.873 362 190 16 64 406 137 464 1.70e-40 150
MS.gene011443.t1 MTR_4g125060 30.769 364 204 11 64 406 132 468 7.89e-40 148
MS.gene011443.t1 MTR_4g088030 30.811 370 213 13 41 405 15 346 9.64e-40 145
MS.gene011443.t1 MTR_8g069790 30.163 368 212 13 64 412 178 519 1.37e-39 149
MS.gene011443.t1 MTR_4g125060 31.319 364 202 12 64 406 200 536 1.65e-39 149
MS.gene011443.t1 MTR_4g125060 30.769 364 204 11 64 406 171 507 1.78e-39 148
MS.gene011443.t1 MTR_8g093530 28.652 356 211 11 64 407 90 414 1.40e-38 144
MS.gene011443.t1 MTR_5g033800 30.435 368 209 16 64 411 145 485 2.96e-38 144
MS.gene011443.t1 MTR_3g021090 30.259 347 204 12 64 405 31 344 2.17e-37 140
MS.gene011443.t1 MTR_3g035830 28.902 346 210 10 64 405 31 344 5.75e-37 138
MS.gene011443.t1 MTR_3g021060 31.322 348 199 14 64 405 46 359 6.68e-37 138
MS.gene011443.t1 MTR_3g464570 30.966 352 211 13 64 405 43 372 8.34e-37 138
MS.gene011443.t1 MTR_4g016940 30.220 364 224 15 59 409 81 427 1.30e-36 139
MS.gene011443.t1 MTR_1g050448 27.926 376 218 11 45 409 42 375 2.14e-36 137
MS.gene011443.t1 MTR_1g112210 29.752 363 191 14 64 405 59 378 4.50e-36 136
MS.gene011443.t1 MTR_4g087730 29.971 347 205 11 64 405 45 358 7.08e-36 135
MS.gene011443.t1 MTR_3g049330 31.667 360 202 13 55 405 22 346 1.27e-35 134
MS.gene011443.t1 MTR_8g093870 27.273 385 229 10 46 406 112 469 3.60e-35 135
MS.gene011443.t1 MTR_3g070900 30.748 361 210 18 64 405 106 445 4.71e-35 135
MS.gene011443.t1 MTR_4g014440 30.376 372 224 15 52 405 49 403 6.98e-35 134
MS.gene011443.t1 MTR_3g090120 29.477 363 207 15 64 409 129 459 1.94e-34 133
MS.gene011443.t1 MTR_2g091175 31.034 348 199 14 64 405 50 362 1.94e-34 132
MS.gene011443.t1 MTR_1g112210 29.363 361 182 14 64 405 59 365 2.83e-34 131
MS.gene011443.t1 MTR_7g068920 30.220 364 224 15 59 409 75 421 3.02e-34 132
MS.gene011443.t1 MTR_8g463790 27.731 357 233 10 64 411 71 411 4.50e-34 132
MS.gene011443.t1 MTR_5g082880 30.000 360 217 14 64 405 165 507 2.33e-33 131
MS.gene011443.t1 MTR_7g094750 32.530 249 144 9 64 299 91 328 5.35e-32 124
MS.gene011443.t1 MTR_7g070060 27.078 421 240 17 10 405 27 405 2.62e-31 124
MS.gene011443.t1 MTR_2g073510 28.490 351 216 11 64 409 33 353 2.66e-31 122
MS.gene011443.t1 MTR_5g083260 27.855 359 223 14 64 406 83 421 2.76e-31 124
MS.gene011443.t1 MTR_4g079700 31.006 358 194 18 64 409 47 363 3.93e-31 122
MS.gene011443.t1 MTR_3g071650 26.437 435 263 16 4 406 18 427 5.32e-31 123
MS.gene011443.t1 MTR_4g102570 48.750 160 59 7 181 328 2 150 7.14e-31 116
MS.gene011443.t1 MTR_2g015720 28.056 360 226 9 52 409 30 358 7.58e-31 122
MS.gene011443.t1 MTR_6g016240 28.184 369 215 12 64 407 71 414 1.45e-29 119
MS.gene011443.t1 MTR_2g073500 29.213 356 197 14 63 405 28 341 1.92e-29 117
MS.gene011443.t1 MTR_4g014430 27.151 372 234 16 53 409 97 446 5.02e-29 118
MS.gene011443.t1 MTR_1g111010 30.163 368 185 18 60 409 28 341 1.80e-28 115
MS.gene011443.t1 MTR_7g019140 44.000 125 56 3 30 154 58 168 4.37e-28 109
MS.gene011443.t1 MTR_4g092830 26.944 360 234 13 64 409 48 392 5.45e-26 109
MS.gene011443.t1 MTR_5g034810 29.630 351 201 15 64 405 35 348 2.60e-25 106
MS.gene011443.t1 MTR_5g033800 33.708 178 106 7 64 233 145 318 3.56e-24 103
MS.gene011443.t1 MTR_3g464570 28.223 287 175 12 129 405 3 268 4.04e-19 87.0
MS.gene011443.t1 MTR_7g070060 26.518 313 182 13 117 405 15 303 1.40e-18 86.3
MS.gene011443.t1 MTR_7g070060 30.729 192 103 7 10 194 27 195 2.59e-18 85.5
MS.gene011443.t1 MTR_7g019130 33.871 124 75 4 186 304 4 125 2.52e-17 79.0
MS.gene011443.t1 MTR_7g117160 31.868 182 74 9 227 408 58 189 7.72e-15 73.2
MS.gene011443.t1 MTR_0929s0010 46.269 67 29 2 47 113 68 127 8.04e-12 62.8
MS.gene011443.t1 MTR_0929s0020 35.714 70 42 1 186 252 4 73 8.85e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene011443.t1 AT1G70230 52.707 351 151 7 64 413 79 415 2.20e-135 395
MS.gene011443.t1 AT5G15890 41.143 350 190 10 64 410 187 523 7.43e-87 274
MS.gene011443.t1 AT1G01430 37.343 399 232 9 31 415 59 453 1.44e-86 271
MS.gene011443.t1 AT4G01080 40.571 350 190 10 64 407 91 428 5.00e-85 266
MS.gene011443.t1 AT4G23790 36.211 417 245 10 10 409 15 427 1.34e-83 263
MS.gene011443.t1 AT4G11090 36.253 411 244 10 11 409 21 425 6.46e-83 261
MS.gene011443.t1 AT3G02440 36.198 384 221 10 40 410 97 469 5.82e-82 260
MS.gene011443.t1 AT3G02440 36.198 384 221 10 40 410 105 477 9.22e-82 259
MS.gene011443.t1 AT5G15900 38.095 357 204 11 64 413 67 413 1.97e-81 257
MS.gene011443.t1 AT5G15900 38.095 357 204 11 64 413 86 432 3.68e-81 257
MS.gene011443.t1 AT3G28150 36.901 355 205 9 64 411 70 412 7.20e-80 253
MS.gene011443.t1 AT4G25360 34.908 381 221 12 47 411 157 526 4.48e-66 220
MS.gene011443.t1 AT4G25360 34.908 381 221 12 47 411 157 526 4.48e-66 220
MS.gene011443.t1 AT5G51640 33.681 383 223 13 47 411 121 490 5.23e-61 206
MS.gene011443.t1 AT5G19160 34.426 366 216 10 64 417 99 452 1.62e-52 182
MS.gene011443.t1 AT3G06080 34.133 375 202 15 64 417 103 453 4.94e-45 162
MS.gene011443.t1 AT3G12060 32.703 370 206 17 64 414 196 541 1.61e-43 160
MS.gene011443.t1 AT5G49340 30.886 395 220 16 42 409 73 441 3.22e-43 157
MS.gene011443.t1 AT5G06230 32.708 373 210 15 52 405 10 360 4.81e-43 155
MS.gene011443.t1 AT5G06230 32.708 373 210 15 52 405 51 401 7.93e-43 155
MS.gene011443.t1 AT1G48880 32.410 361 187 15 64 406 115 436 9.07e-43 156
MS.gene011443.t1 AT5G06700 32.708 373 202 17 64 414 255 600 1.30e-42 158
MS.gene011443.t1 AT5G58600 29.539 369 203 11 64 409 66 400 4.91e-42 153
MS.gene011443.t1 AT5G64020 31.335 367 210 13 64 406 57 405 9.25e-41 150
MS.gene011443.t1 AT5G20590 29.945 364 204 18 64 405 141 475 8.12e-40 149
MS.gene011443.t1 AT2G30010 30.458 371 203 12 64 409 56 396 8.69e-40 147
MS.gene011443.t1 AT3G11570 31.765 340 196 14 80 405 98 415 1.24e-39 147
MS.gene011443.t1 AT2G30010 30.458 371 203 12 64 409 99 439 1.66e-39 147
MS.gene011443.t1 AT3G62390 29.820 389 225 17 35 405 109 467 2.08e-39 147
MS.gene011443.t1 AT1G60790 30.214 374 194 15 64 405 190 528 3.46e-39 148
MS.gene011443.t1 AT3G14850 32.194 351 203 12 64 409 34 354 7.98e-38 140
MS.gene011443.t1 AT2G34070 29.412 357 211 13 60 409 61 383 1.99e-37 140
MS.gene011443.t1 AT2G40160 28.000 425 265 18 4 405 13 419 5.47e-37 140
MS.gene011443.t1 AT2G40160 28.000 425 265 18 4 405 13 419 6.93e-37 140
MS.gene011443.t1 AT2G14530 28.997 369 215 12 64 409 62 406 8.36e-37 139
MS.gene011443.t1 AT5G19160 36.694 248 133 9 64 298 99 335 2.19e-35 134
MS.gene011443.t1 AT3G55990 30.585 376 217 18 52 405 126 479 6.96e-35 135
MS.gene011443.t1 AT2G31110 28.871 381 222 15 41 405 11 358 1.02e-34 132
MS.gene011443.t1 AT1G73140 30.000 360 209 17 64 405 67 401 1.63e-34 133
MS.gene011443.t1 AT5G20680 26.776 366 222 8 64 409 118 457 4.89e-34 132
MS.gene011443.t1 AT2G37720 28.804 368 206 11 64 406 143 479 5.59e-34 132
MS.gene011443.t1 AT2G38320 25.420 417 275 12 1 405 8 400 7.87e-34 131
MS.gene011443.t1 AT5G20680 26.776 366 222 8 64 409 194 533 9.64e-34 132
MS.gene011443.t1 AT5G01360 26.836 354 234 13 64 406 36 375 9.69e-34 130
MS.gene011443.t1 AT5G20680 26.776 366 222 8 64 409 212 551 9.72e-34 132
MS.gene011443.t1 AT5G20680 26.776 366 222 8 64 409 212 551 9.72e-34 132
MS.gene011443.t1 AT5G20680 26.776 366 222 8 64 409 212 551 9.72e-34 132
MS.gene011443.t1 AT5G20680 26.776 366 222 8 64 409 212 551 9.72e-34 132
MS.gene011443.t1 AT1G29050 26.724 348 223 10 64 409 61 378 2.47e-33 129
MS.gene011443.t1 AT5G01360 26.836 354 234 13 64 406 92 431 2.53e-33 130
MS.gene011443.t1 AT2G40150 27.005 374 252 12 46 405 50 416 8.94e-33 128
MS.gene011443.t1 AT2G40320 27.654 358 225 14 64 406 81 419 1.30e-32 128
MS.gene011443.t1 AT2G30900 30.423 355 198 15 64 405 43 361 1.65e-32 126
MS.gene011443.t1 AT2G42570 27.968 379 223 13 42 409 25 364 4.76e-32 125
MS.gene011443.t1 AT3G11030 27.901 362 220 15 64 406 106 445 2.19e-31 125
MS.gene011443.t1 AT5G01620 25.737 373 217 13 64 409 110 449 9.36e-31 123
MS.gene011443.t1 AT5G01620 25.737 373 217 13 64 409 110 449 9.36e-31 123
MS.gene011443.t1 AT3G54260 27.528 356 216 15 64 409 51 374 9.97e-31 122
MS.gene011443.t1 AT5G01620 25.737 373 217 13 64 409 118 457 1.03e-30 123
MS.gene011443.t1 AT1G78710 30.137 365 190 16 64 409 38 356 1.24e-30 121
MS.gene011443.t1 AT5G64470 26.933 375 212 14 63 409 54 394 3.71e-30 120
MS.gene011443.t1 AT5G64470 27.273 374 218 14 63 409 54 400 5.61e-30 120
MS.gene011443.t1 AT2G38320 26.087 322 216 7 95 405 7 317 1.60e-29 117
MS.gene011443.t1 AT3G06080 34.677 248 138 10 64 298 103 339 3.45e-28 114
MS.gene011443.t1 AT5G58600 29.317 249 136 7 64 297 66 289 2.26e-25 105
MS.gene011443.t1 AT5G20590 32.000 200 114 9 64 246 141 335 6.14e-25 105
MS.gene011443.t1 AT3G62390 29.433 282 167 11 35 300 109 374 7.21e-25 105
MS.gene011443.t1 AT2G40160 30.808 198 126 6 4 194 13 206 1.07e-24 103
MS.gene011443.t1 AT5G06230 31.557 244 146 8 52 282 51 286 3.13e-23 99.8
MS.gene011443.t1 AT2G31110 31.280 211 122 7 41 238 11 211 3.16e-23 98.6
MS.gene011443.t1 AT1G78710 28.161 348 166 14 64 409 31 296 2.85e-22 96.7
MS.gene011443.t1 AT2G30900 34.659 176 101 7 64 235 43 208 2.40e-21 93.6
MS.gene011443.t1 AT5G64470 26.071 280 168 10 63 320 54 316 1.04e-18 87.0
MS.gene011443.t1 AT5G64470 24.820 278 160 8 63 313 54 309 1.64e-18 86.3
MS.gene011443.t1 AT2G34070 27.660 282 164 12 136 410 2 250 2.91e-18 84.3

Find 88 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCCATTTGCAAACTTTA+AGG 0.089875 8.2:+21094317 None:intergenic
GTTTGTCTTCAATGAGTTTA+AGG 0.143671 8.2:+21095063 None:intergenic
AAATACATCTTATTATAATT+TGG 0.160255 8.2:+21094834 None:intergenic
AAATTATAATAAGATGTATT+TGG 0.209465 8.2:-21094832 MS.gene011443:CDS
TTTCTGTATGGCTTAATCTT+TGG 0.232960 8.2:+21094513 None:intergenic
GAATTATCATGCATTGAAAA+AGG 0.238491 8.2:-21094649 MS.gene011443:CDS
CTTGATAGCTTGCAATTGTT+AGG 0.287235 8.2:+21095104 None:intergenic
ATGCAAGTGCTTCCTTATAT+TGG 0.319566 8.2:-21094899 MS.gene011443:CDS
TTTGAAGGTGATTGGGATAA+GGG 0.344564 8.2:-21094546 MS.gene011443:CDS
AGATTAAGCCATACAGAAAC+AGG 0.345678 8.2:-21094509 MS.gene011443:CDS
ACTCCATTTGCAAACTTTAA+GGG 0.351417 8.2:+21094318 None:intergenic
TCCTTCTCCCTGTTTCTGTA+TGG 0.364814 8.2:+21094501 None:intergenic
TGCTTGAAATCATGAAGAAA+TGG 0.365942 8.2:-21094224 MS.gene011443:CDS
CTAACAATTGCAAGCTATCA+AGG 0.373855 8.2:-21095103 MS.gene011443:CDS
TGTTATAGTTGTGCTGATCT+TGG 0.379290 8.2:-21094693 MS.gene011443:CDS
GGATATTGATGAGATGGATT+TGG 0.391087 8.2:-21094784 MS.gene011443:CDS
CCGATGATGAGTATCTATAT+TGG 0.392502 8.2:-21095136 MS.gene011443:CDS
TCTTGTCAATTATGCTATCA+AGG 0.397068 8.2:+21094613 None:intergenic
TACATAGAAGTGACTCATAT+TGG 0.398521 8.2:+21095006 None:intergenic
GAAATCTTCAATTTACTATG+AGG 0.398608 8.2:-21094733 MS.gene011443:CDS
GCTCTTCACTCTTCAATACT+TGG 0.401832 8.2:+21094192 None:intergenic
GATCTTGGTGGTAATGAAAC+TGG 0.403049 8.2:-21094678 MS.gene011443:CDS
TCAAAACCTTGTACAAGAAA+AGG 0.406007 8.2:+21094873 None:intergenic
TGGGTGTTTCAAAGTTTCGC+TGG 0.415567 8.2:-21095693 MS.gene011443:CDS
CATGACCCTTTGTGTATACA+AGG 0.416243 8.2:+21094958 None:intergenic
CCCTTTGTGTATACAAGGTT+AGG 0.418054 8.2:+21094963 None:intergenic
TGGATGTTGTTGATGAGAAA+TGG 0.427589 8.2:-21094812 MS.gene011443:CDS
GCTTGAAATCATGAAGAAAT+GGG 0.429048 8.2:-21094223 MS.gene011443:CDS
TTGAGACCAGATGGTCATCC+AGG 0.429326 8.2:-21094354 MS.gene011443:CDS
TCATGGTAGTTGAGTTGAAA+TGG 0.431536 8.2:+21095587 None:intergenic
ATCTCATTCCATGTATCTAT+AGG 0.431656 8.2:+21094249 None:intergenic
TCGTGCTATCAATTGGTCAT+TGG 0.434054 8.2:-21094761 MS.gene011443:CDS
CATGGTAGTTGAGTTGAAAT+GGG 0.441487 8.2:+21095588 None:intergenic
TGTACACATTGGTGTTTACC+AGG 0.442530 8.2:-21094273 MS.gene011443:CDS
TTGATAGCTTGCAATTGTTA+GGG 0.449291 8.2:+21095105 None:intergenic
TAAAGACCTGGATGACCATC+TGG 0.453635 8.2:+21094348 None:intergenic
AAATGGAAGGGTTAGGTTTG+AGG 0.454302 8.2:-21094403 MS.gene011443:CDS
AATGAGTTTAAGGAAAGTGT+TGG 0.459155 8.2:+21095073 None:intergenic
GATTTGGTCGTGCTATCAAT+TGG 0.465164 8.2:-21094768 MS.gene011443:CDS
AAGAAAAGGAGACCAATATA+AGG 0.465384 8.2:+21094887 None:intergenic
CGTCCCTTAAAGTTTGCAAA+TGG 0.478180 8.2:-21094321 MS.gene011443:CDS
CACCAATGTGTACAATCATT+CGG 0.481222 8.2:+21094282 None:intergenic
CAATATAGATACTCATCATC+GGG 0.484814 8.2:+21095137 None:intergenic
GACAAAGGATATTGATGAGA+TGG 0.489508 8.2:-21094790 MS.gene011443:CDS
ATGAGTTTAAGGAAAGTGTT+GGG 0.490796 8.2:+21095074 None:intergenic
AAATCTTCAATTTACTATGA+GGG 0.491548 8.2:-21094732 MS.gene011443:CDS
TCCAACTCTACACCTTCGTC+AGG 0.492975 8.2:-21095559 MS.gene011443:intron
TGTAGAGTTGGAGATGATCA+TGG 0.493631 8.2:+21095570 None:intergenic
GGAAGATCAAATTCTGAAGA+TGG 0.496762 8.2:+21095610 None:intergenic
TTGTTGGTGATTCTTTGGCT+AGG 0.497790 8.2:-21095031 MS.gene011443:CDS
GGGTGTTTCAAAGTTTCGCT+GGG 0.506462 8.2:-21095692 MS.gene011443:CDS
CTCCCATGTGATTTCTCCAA+TGG 0.510688 8.2:-21095254 MS.gene011443:CDS
ATGAGTATCTATATTGGAGA+TGG 0.514623 8.2:-21095130 MS.gene011443:CDS
TGAAGAAGTTGAAGCTGCTA+AGG 0.519001 8.2:-21094439 MS.gene011443:CDS
CCTAACCTTGTATACACAAA+GGG 0.520288 8.2:-21094963 MS.gene011443:CDS
GTCATGGATCCATGTTCCAT+TGG 0.520938 8.2:+21095238 None:intergenic
ACCTGACGAAGGTGTAGAGT+TGG 0.524674 8.2:+21095558 None:intergenic
TTCCATGTATCTATAGGCCC+TGG 0.529916 8.2:+21094255 None:intergenic
TATAGTTGTGCTGATCTTGG+TGG 0.538478 8.2:-21094690 MS.gene011443:CDS
CCAATATAGATACTCATCAT+CGG 0.547581 8.2:+21095136 None:intergenic
TTATGCATGGTGATATGAGA+AGG 0.549324 8.2:-21094470 MS.gene011443:CDS
ATGAGAATAGATTTAGAAAG+TGG 0.549699 8.2:-21094938 MS.gene011443:CDS
GAAGGTGATTGGGATAAGGG+TGG 0.571866 8.2:-21094543 MS.gene011443:CDS
CATTGTACAAAGGACCACTT+AGG 0.572103 8.2:+21095216 None:intergenic
GTTGAAGCTGCTAAGGTGAA+AGG 0.577562 8.2:-21094432 MS.gene011443:CDS
CTTGTCAATTATGCTATCAA+GGG 0.584390 8.2:+21094614 None:intergenic
GTGATTTCTCCAATGGAACA+TGG 0.584806 8.2:-21095247 MS.gene011443:CDS
CATGTTGTTCCATTGTACAA+AGG 0.585508 8.2:+21095206 None:intergenic
ACAAAGGACCACTTAGGTCA+TGG 0.588556 8.2:+21095222 None:intergenic
GCACAAAATTGCATGAAACA+TGG 0.589654 8.2:-21095164 MS.gene011443:CDS
TCACCTTCAAAATGATGTGG+TGG 0.596718 8.2:+21094558 None:intergenic
ATGGTAGTTGAGTTGAAATG+GGG 0.603017 8.2:+21095589 None:intergenic
CTAAGTGGTCCTTTGTACAA+TGG 0.611732 8.2:-21095215 MS.gene011443:CDS
TACCAGGGCCTATAGATACA+TGG 0.613847 8.2:-21094257 MS.gene011443:CDS
ACCTAACCTTGTATACACAA+AGG 0.614223 8.2:-21094964 MS.gene011443:CDS
GTTCCATTGGAGAAATCACA+TGG 0.625175 8.2:+21095251 None:intergenic
GCCATACAGAAACAGGGAGA+AGG 0.625719 8.2:-21094502 MS.gene011443:CDS
CAATCACCTTCAAAATGATG+TGG 0.630714 8.2:+21094555 None:intergenic
ACATGGATCCATGACCTAAG+TGG 0.635398 8.2:-21095230 MS.gene011443:CDS
AAGGGACGTGTATAAAGACC+TGG 0.643670 8.2:+21094336 None:intergenic
TATGCATGGTGATATGAGAA+GGG 0.650241 8.2:-21094469 MS.gene011443:CDS
AAGGAACTTGAAGTTATGCA+TGG 0.652940 8.2:-21094483 MS.gene011443:CDS
GTACACATTGGTGTTTACCA+GGG 0.654100 8.2:-21094272 MS.gene011443:CDS
TGTTGATGAGAAATGGACAA+AGG 0.687196 8.2:-21094805 MS.gene011443:CDS
GATTAAGCCATACAGAAACA+GGG 0.687255 8.2:-21094508 MS.gene011443:CDS
TTCCATTGGAGAAATCACAT+GGG 0.718347 8.2:+21095252 None:intergenic
TGCCGAATGATTGTACACAT+TGG 0.726351 8.2:-21094284 MS.gene011443:CDS
TAATGATGAATACCTGACGA+AGG 0.745175 8.2:+21095547 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATACATCTTATTATAATT+TGG + chr8.2:21095063-21095082 None:intergenic 10.0%
!! AAATTATAATAAGATGTATT+TGG - chr8.2:21095062-21095081 MS.gene011443:CDS 10.0%
!!! AAAATCATATTTTTACAAAT+TGG - chr8.2:21094476-21094495 MS.gene011443:CDS 10.0%
!!! ATATGATTTTTAATAAGATG+AGG + chr8.2:21094378-21094397 None:intergenic 15.0%
!! AAATCTTCAATTTACTATGA+GGG - chr8.2:21095162-21095181 MS.gene011443:CDS 20.0%
!! AACATTGAATCAATTATTCT+TGG + chr8.2:21094585-21094604 None:intergenic 20.0%
!!! TGTTGCTAATTAAGATTTTA+AGG + chr8.2:21094509-21094528 None:intergenic 20.0%
! ATGAGAATAGATTTAGAAAG+TGG - chr8.2:21094956-21094975 MS.gene011443:CDS 25.0%
! GAAATCTTCAATTTACTATG+AGG - chr8.2:21095161-21095180 MS.gene011443:CDS 25.0%
! GAATTATCATGCATTGAAAA+AGG - chr8.2:21095245-21095264 MS.gene011443:CDS 25.0%
!! TGTTGCTAATTCAGATTTTA+AGG + chr8.2:21094414-21094433 None:intergenic 25.0%
!!! ACAAGGTTTTGAAAAATCAA+AGG - chr8.2:21095032-21095051 MS.gene011443:CDS 25.0%
!!! ATGTTCATTTGTTGTAGAAA+AGG - chr8.2:21094600-21094619 MS.gene011443:CDS 25.0%
!!! GTTTTGAAAAATCAAAGGAA+GGG - chr8.2:21095037-21095056 MS.gene011443:CDS 25.0%
AACTCCATTTGCAAACTTTA+AGG + chr8.2:21095580-21095599 None:intergenic 30.0%
AAGAAAAGGAGACCAATATA+AGG + chr8.2:21095010-21095029 None:intergenic 30.0%
AAGGTGAAAGGTAGAAAAAA+TGG - chr8.2:21095474-21095493 MS.gene011443:intron 30.0%
ACTCCATTTGCAAACTTTAA+GGG + chr8.2:21095579-21095598 None:intergenic 30.0%
ATCTCATTCCATGTATCTAT+AGG + chr8.2:21095648-21095667 None:intergenic 30.0%
ATGAGTATCTATATTGGAGA+TGG - chr8.2:21094764-21094783 MS.gene011443:CDS 30.0%
ATGCATTGAAAAAGGCTTTA+AGG - chr8.2:21095253-21095272 MS.gene011443:CDS 30.0%
CAATATAGATACTCATCATC+GGG + chr8.2:21094760-21094779 None:intergenic 30.0%
CCAATATAGATACTCATCAT+CGG + chr8.2:21094761-21094780 None:intergenic 30.0%
CTTGTCAATTATGCTATCAA+GGG + chr8.2:21095283-21095302 None:intergenic 30.0%
GAAAGGTAGAAAAAATGGAA+GGG - chr8.2:21095479-21095498 MS.gene011443:intron 30.0%
GCTTGAAATCATGAAGAAAT+GGG - chr8.2:21095671-21095690 MS.gene011443:CDS 30.0%
GTTTGTCTTCAATGAGTTTA+AGG + chr8.2:21094834-21094853 None:intergenic 30.0%
TACATAGAAGTGACTCATAT+TGG + chr8.2:21094891-21094910 None:intergenic 30.0%
TCAAAACCTTGTACAAGAAA+AGG + chr8.2:21095024-21095043 None:intergenic 30.0%
TCTTGTCAATTATGCTATCA+AGG + chr8.2:21095284-21095303 None:intergenic 30.0%
TGAAAGGTAGAAAAAATGGA+AGG - chr8.2:21095478-21095497 MS.gene011443:intron 30.0%
TGCTTGAAATCATGAAGAAA+TGG - chr8.2:21095670-21095689 MS.gene011443:CDS 30.0%
TTTCTGTATGGCTTAATCTT+TGG + chr8.2:21095384-21095403 None:intergenic 30.0%
! AATGAGTTTAAGGAAAGTGT+TGG + chr8.2:21094824-21094843 None:intergenic 30.0%
! TTGATAGCTTGCAATTGTTA+GGG + chr8.2:21094792-21094811 None:intergenic 30.0%
!! ACATCATTTTGAAGGTGATT+GGG - chr8.2:21095341-21095360 MS.gene011443:intron 30.0%
!! ATGAGTTTAAGGAAAGTGTT+GGG + chr8.2:21094823-21094842 None:intergenic 30.0%
!!! GGTTTTGAAAAATCAAAGGA+AGG - chr8.2:21095036-21095055 MS.gene011443:CDS 30.0%
!!! TATGAGGGTGATTTAGTTTT+AGG - chr8.2:21095177-21095196 MS.gene011443:CDS 30.0%
AAGGAACTTGAAGTTATGCA+TGG - chr8.2:21095411-21095430 MS.gene011443:intron 35.0%
ACCTAACCTTGTATACACAA+AGG - chr8.2:21094930-21094949 MS.gene011443:CDS 35.0%
AGATTAAGCCATACAGAAAC+AGG - chr8.2:21095385-21095404 MS.gene011443:intron 35.0%
ATGCAAGTGCTTCCTTATAT+TGG - chr8.2:21094995-21095014 MS.gene011443:CDS 35.0%
CAATCACCTTCAAAATGATG+TGG + chr8.2:21095342-21095361 None:intergenic 35.0%
CACCAATGTGTACAATCATT+CGG + chr8.2:21095615-21095634 None:intergenic 35.0%
CATGTTGTTCCATTGTACAA+AGG + chr8.2:21094691-21094710 None:intergenic 35.0%
CCGATGATGAGTATCTATAT+TGG - chr8.2:21094758-21094777 MS.gene011443:CDS 35.0%
CCTAACCTTGTATACACAAA+GGG - chr8.2:21094931-21094950 MS.gene011443:CDS 35.0%
CTAACAATTGCAAGCTATCA+AGG - chr8.2:21094791-21094810 MS.gene011443:CDS 35.0%
GACAAAGGATATTGATGAGA+TGG - chr8.2:21095104-21095123 MS.gene011443:CDS 35.0%
GATTAAGCCATACAGAAACA+GGG - chr8.2:21095386-21095405 MS.gene011443:intron 35.0%
GCACAAAATTGCATGAAACA+TGG - chr8.2:21094730-21094749 MS.gene011443:CDS 35.0%
GTAGAAAAAATGGAAGGGTT+AGG - chr8.2:21095484-21095503 MS.gene011443:intron 35.0%
TAATGATGAATACCTGACGA+AGG + chr8.2:21094350-21094369 None:intergenic 35.0%
TATGCATGGTGATATGAGAA+GGG - chr8.2:21095425-21095444 MS.gene011443:intron 35.0%
TTATGCATGGTGATATGAGA+AGG - chr8.2:21095424-21095443 MS.gene011443:intron 35.0%
TTCCATTGGAGAAATCACAT+GGG + chr8.2:21094645-21094664 None:intergenic 35.0%
TTTGAAGGTGATTGGGATAA+GGG - chr8.2:21095348-21095367 MS.gene011443:intron 35.0%
! CTTGATAGCTTGCAATTGTT+AGG + chr8.2:21094793-21094812 None:intergenic 35.0%
! GACAAACACATAGCTTTTGT+TGG - chr8.2:21094847-21094866 MS.gene011443:CDS 35.0%
! GGAAGATCAAATTCTGAAGA+TGG + chr8.2:21094287-21094306 None:intergenic 35.0%
! GGATATTGATGAGATGGATT+TGG - chr8.2:21095110-21095129 MS.gene011443:CDS 35.0%
! TGGATGTTGTTGATGAGAAA+TGG - chr8.2:21095082-21095101 MS.gene011443:CDS 35.0%
! TGTTATAGTTGTGCTGATCT+TGG - chr8.2:21095201-21095220 MS.gene011443:CDS 35.0%
! TGTTGATGAGAAATGGACAA+AGG - chr8.2:21095089-21095108 MS.gene011443:CDS 35.0%
! TTTCCACCACATCATTTTGA+AGG - chr8.2:21095333-21095352 MS.gene011443:intron 35.0%
!! ATGGTAGTTGAGTTGAAATG+GGG + chr8.2:21094308-21094327 None:intergenic 35.0%
!! CACATCATTTTGAAGGTGAT+TGG - chr8.2:21095340-21095359 MS.gene011443:intron 35.0%
!! CATGGTAGTTGAGTTGAAAT+GGG + chr8.2:21094309-21094328 None:intergenic 35.0%
!! TCATGGTAGTTGAGTTGAAA+TGG + chr8.2:21094310-21094329 None:intergenic 35.0%
!! TTTTGAAGGTGATTGGGATA+AGG - chr8.2:21095347-21095366 MS.gene011443:intron 35.0%
!!! AGCTTTTGTTGGTGATTCTT+TGG - chr8.2:21094858-21094877 MS.gene011443:CDS 35.0%
!!! GTGGTAATGAAACTGGTTTT+CGG - chr8.2:21095223-21095242 MS.gene011443:CDS 35.0%
!!! TTAGCATTTTTGAGACCAGA+TGG - chr8.2:21095531-21095550 MS.gene011443:intron 35.0%
AAATGGAAGGGTTAGGTTTG+AGG - chr8.2:21095491-21095510 MS.gene011443:intron 40.0%
CATGACCCTTTGTGTATACA+AGG + chr8.2:21094939-21094958 None:intergenic 40.0%
CATTGTACAAAGGACCACTT+AGG + chr8.2:21094681-21094700 None:intergenic 40.0%
CCCTTTGTGTATACAAGGTT+AGG + chr8.2:21094934-21094953 None:intergenic 40.0%
CGTCCCTTAAAGTTTGCAAA+TGG - chr8.2:21095573-21095592 MS.gene011443:CDS 40.0%
CTAAGTGGTCCTTTGTACAA+TGG - chr8.2:21094679-21094698 MS.gene011443:CDS 40.0%
GTGATTTCTCCAATGGAACA+TGG - chr8.2:21094647-21094666 MS.gene011443:CDS 40.0%
GTTCCATTGGAGAAATCACA+TGG + chr8.2:21094646-21094665 None:intergenic 40.0%
TCACCTTCAAAATGATGTGG+TGG + chr8.2:21095339-21095358 None:intergenic 40.0%
TCGTGCTATCAATTGGTCAT+TGG - chr8.2:21095133-21095152 MS.gene011443:CDS 40.0%
TGAAGAAGTTGAAGCTGCTA+AGG - chr8.2:21095455-21095474 MS.gene011443:intron 40.0%
TGCCGAATGATTGTACACAT+TGG - chr8.2:21095610-21095629 MS.gene011443:CDS 40.0%
TGTAGAGTTGGAGATGATCA+TGG + chr8.2:21094327-21094346 None:intergenic 40.0%
! GATCTTGGTGGTAATGAAAC+TGG - chr8.2:21095216-21095235 MS.gene011443:CDS 40.0%
! GATTTGGTCGTGCTATCAAT+TGG - chr8.2:21095126-21095145 MS.gene011443:CDS 40.0%
! GTACACATTGGTGTTTACCA+GGG - chr8.2:21095622-21095641 MS.gene011443:CDS 40.0%
! TATAGTTGTGCTGATCTTGG+TGG - chr8.2:21095204-21095223 MS.gene011443:CDS 40.0%
! TGGTCTCCTTTTCTTGTACA+AGG - chr8.2:21095015-21095034 MS.gene011443:CDS 40.0%
! TGTACACATTGGTGTTTACC+AGG - chr8.2:21095621-21095640 MS.gene011443:CDS 40.0%
!! TTGTTGGTGATTCTTTGGCT+AGG - chr8.2:21094863-21094882 MS.gene011443:CDS 40.0%
AAGGGACGTGTATAAAGACC+TGG + chr8.2:21095561-21095580 None:intergenic 45.0%
ACAAAGGACCACTTAGGTCA+TGG + chr8.2:21094675-21094694 None:intergenic 45.0%
ACATGGATCCATGACCTAAG+TGG - chr8.2:21094664-21094683 MS.gene011443:CDS 45.0%
CTCCCATGTGATTTCTCCAA+TGG - chr8.2:21094640-21094659 MS.gene011443:CDS 45.0%
GGGTGTTTCAAAGTTTCGCT+GGG - chr8.2:21094202-21094221 MS.gene011443:CDS 45.0%
GTCATGGATCCATGTTCCAT+TGG + chr8.2:21094659-21094678 None:intergenic 45.0%
GTTGAAGCTGCTAAGGTGAA+AGG - chr8.2:21095462-21095481 MS.gene011443:intron 45.0%
TAAAGACCTGGATGACCATC+TGG + chr8.2:21095549-21095568 None:intergenic 45.0%
TACCAGGGCCTATAGATACA+TGG - chr8.2:21095637-21095656 MS.gene011443:CDS 45.0%
TGGGTGTTTCAAAGTTTCGC+TGG - chr8.2:21094201-21094220 MS.gene011443:CDS 45.0%
TTCCATGTATCTATAGGCCC+TGG + chr8.2:21095642-21095661 None:intergenic 45.0%
!! TCCTTCTCCCTGTTTCTGTA+TGG + chr8.2:21095396-21095415 None:intergenic 45.0%
GAAGGTGATTGGGATAAGGG+TGG - chr8.2:21095351-21095370 MS.gene011443:intron 50.0%
GCCATACAGAAACAGGGAGA+AGG - chr8.2:21095392-21095411 MS.gene011443:intron 50.0%
TCCAACTCTACACCTTCGTC+AGG - chr8.2:21094335-21094354 MS.gene011443:CDS 50.0%
TTGAGACCAGATGGTCATCC+AGG - chr8.2:21095540-21095559 MS.gene011443:intron 50.0%
! ACCTGACGAAGGTGTAGAGT+TGG + chr8.2:21094339-21094358 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 21094197 21095719 21094197 ID=MS.gene011443
chr8.2 mRNA 21094197 21095719 21094197 ID=MS.gene011443.t1;Parent=MS.gene011443
chr8.2 exon 21095560 21095719 21095560 ID=MS.gene011443.t1.exon1;Parent=MS.gene011443.t1
chr8.2 CDS 21095560 21095719 21095560 ID=cds.MS.gene011443.t1;Parent=MS.gene011443.t1
chr8.2 exon 21094197 21095299 21094197 ID=MS.gene011443.t1.exon2;Parent=MS.gene011443.t1
chr8.2 CDS 21094197 21095299 21094197 ID=cds.MS.gene011443.t1;Parent=MS.gene011443.t1
Gene Sequence

>MS.gene011443

ATGGTGGGTGTTTCAAAGTTTCGCTGGGCTCTCTGTGCACTTGTTCTCATTGCTCTTAGCATCTACTTCTATTGTGATCGCTTTCTTCCATCTTCAGAATTTGATCTTCCCCATTTCAACTCAACTACCATGATCATCTCCAACTCTACACCTTCGTCAGGTATTCATCATTACATCACCTCATCTTATTAAAAATCATATTTTTACATTTTTTCCTTAAAATCTGAATTAGCAACAAATCAAGTGTTTAAAAAAGCAACAACTTTGTAATATTTATTAAAAATCATATTTTTACAAATTGGTTTTTTTCCTTAAAATCTTAATTAGCAACAAATCAATCTAAGTGTTTAAAAAAGCAACAACTTTGTAATATTTGTTTAGTTTTCCAAGAATAATTGATTCAATGTTCATTTGTTGTAGAAAAGGAGAAGACTTTTCACACTCTCCCATGTGATTTCTCCAATGGAACATGGATCCATGACCTAAGTGGTCCTTTGTACAATGGAACAACATGTAAAACAACAATCAAAGAAGCACAAAATTGCATGAAACATGGAAGACCCGATGATGAGTATCTATATTGGAGATGGAACCCTAACAATTGCAAGCTATCAAGGTTTGAACCCAACACTTTCCTTAAACTCATTGAAGACAAACACATAGCTTTTGTTGGTGATTCTTTGGCTAGGAACCAATATGAGTCACTTCTATGTATGTTGTCAATTGCTTCAAAACCTAACCTTGTATACACAAAGGGTCATGAGAATAGATTTAGAAAGTGGTACTTTGATTCTCACAATGCAAGTGCTTCCTTATATTGGTCTCCTTTTCTTGTACAAGGTTTTGAAAAATCAAAGGAAGGGCCAAATTATAATAAGATGTATTTGGATGTTGTTGATGAGAAATGGACAAAGGATATTGATGAGATGGATTTGGTCGTGCTATCAATTGGTCATTGGTTTTTGAAATCTTCAATTTACTATGAGGGTGATTTAGTTTTAGGTTGTTATAGTTGTGCTGATCTTGGTGGTAATGAAACTGGTTTTCGGAATTATCATGCATTGAAAAAGGCTTTAAGGACTACCCTTGATAGCATAATTGACAAGAGAGTTAATATTGATGTAGTTGTGACAACGTTTCCACCACATCATTTTGAAGGTGATTGGGATAAGGGTGGTGTTTGTCCAAAGATTAAGCCATACAGAAACAGGGAGAAGGAACTTGAAGTTATGCATGGTGATATGAGAAGGGTTGAAATTGAAGAAGTTGAAGCTGCTAAGGTGAAAGGTAGAAAAAATGGAAGGGTTAGGTTTGAGGCACTTGATATAACTAGATTAGCATTTTTGAGACCAGATGGTCATCCAGGTCTTTATACACGTCCCTTAAAGTTTGCAAATGGAGTTCAAAAGCGCATGCCGAATGATTGTACACATTGGTGTTTACCAGGGCCTATAGATACATGGAATGAGATTTTGCTTGAAATCATGAAGAAATGGGAGCAGCATCCAAGTATTGAAGAGTGA
Protein sequence

>MS.gene011443.t1

MVGVSKFRWALCALVLIALSIYFYCDRFLPSSEFDLPHFNSTTMIISNSTPSSEKEKTFHTLPCDFSNGTWIHDLSGPLYNGTTCKTTIKEAQNCMKHGRPDDEYLYWRWNPNNCKLSRFEPNTFLKLIEDKHIAFVGDSLARNQYESLLCMLSIASKPNLVYTKGHENRFRKWYFDSHNASASLYWSPFLVQGFEKSKEGPNYNKMYLDVVDEKWTKDIDEMDLVVLSIGHWFLKSSIYYEGDLVLGCYSCADLGGNETGFRNYHALKKALRTTLDSIIDKRVNIDVVVTTFPPHHFEGDWDKGGVCPKIKPYRNREKELEVMHGDMRRVEIEEVEAAKVKGRKNGRVRFEALDITRLAFLRPDGHPGLYTRPLKFANGVQKRMPNDCTHWCLPGPIDTWNEILLEIMKKWEQHPSIEE