Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012839.t1 | XP_013461828.1 | 97.4 | 387 | 10 | 0 | 1 | 387 | 1 | 387 | 1.70E-217 | 765 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012839.t1 | Q9M439 | 66.8 | 382 | 123 | 2 | 10 | 387 | 7 | 388 | 3.3e-145 | 516.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012839.t1 | A0A072V3G0 | 97.4 | 387 | 10 | 0 | 1 | 387 | 1 | 387 | 1.2e-217 | 765.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene012839 | MS.gene09632 | PPI |
| MS.gene012839 | MS.gene015287 | PPI |
| MS.gene012839 | MS.gene21440 | PPI |
| MS.gene43507 | MS.gene012839 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012839.t1 | MTR_3g103580 | 97.416 | 387 | 10 | 0 | 1 | 387 | 1 | 387 | 0.0 | 780 |
| MS.gene012839.t1 | MTR_3g103580 | 97.429 | 350 | 9 | 0 | 1 | 350 | 1 | 350 | 0.0 | 702 |
| MS.gene012839.t1 | MTR_3g103580 | 98.142 | 323 | 6 | 0 | 65 | 387 | 1 | 323 | 0.0 | 656 |
| MS.gene012839.t1 | MTR_5g014960 | 65.882 | 340 | 116 | 0 | 47 | 386 | 68 | 407 | 2.62e-167 | 474 |
| MS.gene012839.t1 | MTR_4g092590 | 59.770 | 348 | 135 | 3 | 43 | 387 | 12 | 357 | 7.96e-150 | 428 |
| MS.gene012839.t1 | MTR_4g092580 | 57.143 | 336 | 144 | 0 | 52 | 387 | 22 | 357 | 3.07e-146 | 419 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012839.t1 | AT1G10070 | 66.754 | 382 | 123 | 2 | 10 | 387 | 7 | 388 | 0.0 | 527 |
| MS.gene012839.t1 | AT1G10070 | 66.754 | 382 | 123 | 2 | 10 | 387 | 7 | 388 | 0.0 | 527 |
| MS.gene012839.t1 | AT1G10070 | 72.013 | 318 | 89 | 0 | 70 | 387 | 1 | 318 | 3.83e-173 | 485 |
| MS.gene012839.t1 | AT1G10060 | 66.962 | 339 | 112 | 0 | 48 | 386 | 45 | 383 | 1.48e-171 | 484 |
| MS.gene012839.t1 | AT1G10060 | 67.160 | 338 | 111 | 0 | 48 | 385 | 141 | 478 | 3.61e-170 | 484 |
| MS.gene012839.t1 | AT3G49680 | 65.385 | 338 | 115 | 2 | 51 | 386 | 75 | 412 | 5.00e-162 | 461 |
| MS.gene012839.t1 | AT3G49680 | 65.385 | 338 | 115 | 2 | 51 | 386 | 73 | 410 | 5.44e-162 | 461 |
| MS.gene012839.t1 | AT5G65780 | 63.636 | 341 | 123 | 1 | 47 | 386 | 74 | 414 | 8.67e-162 | 460 |
| MS.gene012839.t1 | AT1G10060 | 66.877 | 317 | 105 | 0 | 70 | 386 | 1 | 317 | 3.31e-158 | 447 |
| MS.gene012839.t1 | AT1G10060 | 69.637 | 303 | 92 | 0 | 48 | 350 | 45 | 347 | 6.41e-156 | 444 |
| MS.gene012839.t1 | AT1G10060 | 69.637 | 303 | 92 | 0 | 48 | 350 | 141 | 443 | 1.15e-154 | 444 |
| MS.gene012839.t1 | AT1G50090 | 57.910 | 354 | 146 | 2 | 36 | 387 | 3 | 355 | 4.07e-153 | 436 |
| MS.gene012839.t1 | AT1G50110 | 56.534 | 352 | 151 | 2 | 36 | 387 | 3 | 352 | 2.14e-151 | 432 |
| MS.gene012839.t1 | AT1G50110 | 57.547 | 318 | 134 | 1 | 70 | 387 | 1 | 317 | 1.63e-139 | 400 |
| MS.gene012839.t1 | AT3G19710 | 50.427 | 351 | 173 | 1 | 37 | 387 | 2 | 351 | 1.08e-134 | 389 |
| MS.gene012839.t1 | AT1G10060 | 68.651 | 252 | 79 | 0 | 48 | 299 | 45 | 296 | 3.19e-127 | 369 |
| MS.gene012839.t1 | AT1G10060 | 68.651 | 252 | 79 | 0 | 48 | 299 | 141 | 392 | 7.47e-126 | 369 |
Find 109 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGTGAACTTTCCAAGTTTA+AGG | 0.116340 | 3.3:-85642907 | MS.gene012839:CDS |
| TGTAGTGCTGGCCAAAATTT+TGG | 0.137189 | 3.3:-85641122 | MS.gene012839:CDS |
| CATCACTTTACCTTCTATTC+TGG | 0.200728 | 3.3:+85634560 | None:intergenic |
| ATATGGTACCGGTGCATAAT+TGG | 0.225695 | 3.3:+85637123 | None:intergenic |
| TTAGGAAGTGGTCCGGTTCT+TGG | 0.250877 | 3.3:-85637393 | MS.gene012839:CDS |
| ACCTGTTTGTATTCCTAAAA+TGG | 0.255480 | 3.3:+85634185 | None:intergenic |
| TGCCAGTGATTTGGGTTATC+AGG | 0.275271 | 3.3:-85635979 | MS.gene012839:intron |
| GCTGTCCATGTCCAAAATTT+TGG | 0.281068 | 3.3:+85641111 | None:intergenic |
| CAATTCCGGACCCAACTTAC+AGG | 0.297018 | 3.3:-85641372 | MS.gene012839:intron |
| AAAGCATACAGAAAGGAAAA+TGG | 0.301781 | 3.3:-85637721 | MS.gene012839:CDS |
| CATTGAAATTGCCAGTGATT+TGG | 0.302811 | 3.3:-85635988 | MS.gene012839:CDS |
| ATCATACCTGGCCATAATTT+AGG | 0.308609 | 3.3:+85641045 | None:intergenic |
| TCCGCAGGGGTCCTAAATTA+TGG | 0.310746 | 3.3:-85641056 | MS.gene012839:CDS |
| GATTGGGACAGCCTTGGGTT+TGG | 0.329076 | 3.3:-85641179 | MS.gene012839:CDS |
| GCAAAGGATCGTTGTATCTT+AGG | 0.329627 | 3.3:-85637425 | MS.gene012839:CDS |
| GCCCTCCCCTCTTCCAATTC+CGG | 0.331887 | 3.3:-85641386 | MS.gene012839:CDS |
| CTGTAAGTTGGGTCCGGAAT+TGG | 0.338583 | 3.3:+85641373 | None:intergenic |
| CCCTGTTCGCAACTATTTCA+AGG | 0.338859 | 3.3:-85637328 | MS.gene012839:intron |
| TCTTGTGATTCCGGAAAGTA+TGG | 0.340136 | 3.3:+85636019 | None:intergenic |
| ATCAATGGAACCATACTTTC+CGG | 0.356234 | 3.3:-85636029 | MS.gene012839:CDS |
| ATGTGGATTGGGACAGCCTT+GGG | 0.366715 | 3.3:-85641184 | MS.gene012839:CDS |
| CCTTGACATGTAGGTCTTGA+TGG | 0.367499 | 3.3:-85636713 | MS.gene012839:intron |
| TATCTTAGGCCACTGCTCTT+AGG | 0.375755 | 3.3:-85637411 | MS.gene012839:CDS |
| ATTGAAATTGCCAGTGATTT+GGG | 0.377285 | 3.3:-85635987 | MS.gene012839:CDS |
| TTGGACATGGACAGCTTAAT+CGG | 0.380425 | 3.3:-85641103 | MS.gene012839:CDS |
| ACCTTGAAATAGTTGCGAAC+AGG | 0.388998 | 3.3:+85637327 | None:intergenic |
| TATATGAGGAATGTGTATTC+AGG | 0.397711 | 3.3:+85637357 | None:intergenic |
| AGAAGCAGTTTCCTTAAACT+TGG | 0.398954 | 3.3:+85642896 | None:intergenic |
| GTGGACTATAAAACAGGTTC+TGG | 0.405793 | 3.3:-85634234 | MS.gene012839:CDS |
| TATTATAGTTACCTGTAAGT+TGG | 0.436071 | 3.3:+85641361 | None:intergenic |
| ACTTAATTTGTACGTGGAAG+AGG | 0.438422 | 3.3:-85637194 | MS.gene012839:CDS |
| CGTGCTTCGCAACGCGGGAC+TGG | 0.439406 | 3.3:-85637163 | MS.gene012839:CDS |
| ATTATAGTTACCTGTAAGTT+GGG | 0.439858 | 3.3:+85641362 | None:intergenic |
| ATTGATAGCAGGTGTTGAAA+TGG | 0.440308 | 3.3:+85636046 | None:intergenic |
| AGGTAGTATTGAAGATAAGA+AGG | 0.442292 | 3.3:-85634166 | MS.gene012839:CDS |
| CTTGCACAACTCTTGACAAA+TGG | 0.449586 | 3.3:+85634209 | None:intergenic |
| TACCGGTGCATAATTGGAAA+TGG | 0.455649 | 3.3:+85637129 | None:intergenic |
| CATGGACAGCTTAATCGGTA+TGG | 0.457574 | 3.3:-85641098 | MS.gene012839:CDS |
| GTATCACATACCAGAATAGA+AGG | 0.460502 | 3.3:-85634570 | MS.gene012839:intron |
| AGGAAACTGCTTCTTCGTCC+TGG | 0.467038 | 3.3:-85642887 | MS.gene012839:CDS |
| TGCAGGGTGGACTATAAAAC+AGG | 0.473120 | 3.3:-85634240 | MS.gene012839:intron |
| ATCCGAAAATCCTCTGCTCT+TGG | 0.477180 | 3.3:+85636678 | None:intergenic |
| AATGTGGATTGGGACAGCCT+TGG | 0.478960 | 3.3:-85641185 | MS.gene012839:CDS |
| GTCCGGAATTGGAAGAGGGG+AGG | 0.483252 | 3.3:+85641384 | None:intergenic |
| ATTTCAACACCTGCTATCAA+TGG | 0.483444 | 3.3:-85636044 | MS.gene012839:CDS |
| AGCTCTAGCAAATAAACGTT+GGG | 0.486780 | 3.3:-85637584 | MS.gene012839:intron |
| AGCATCAACAAATTGATCAA+TGG | 0.490126 | 3.3:+85637618 | None:intergenic |
| TAATGTATAGGTTCCTCCAC+CGG | 0.490433 | 3.3:-85637448 | MS.gene012839:intron |
| AATGCTATTCGCATGAAGAT+TGG | 0.496130 | 3.3:-85637670 | MS.gene012839:CDS |
| AGTTACCTGTAAGTTGGGTC+CGG | 0.496236 | 3.3:+85641367 | None:intergenic |
| AACGATCCTTTGCCCGGTGG+AGG | 0.507822 | 3.3:+85637435 | None:intergenic |
| AGATACAACGATCCTTTGCC+CGG | 0.508926 | 3.3:+85637429 | None:intergenic |
| AGGCCACTGCTCTTAGGAAG+TGG | 0.509303 | 3.3:-85637405 | MS.gene012839:CDS |
| AAGCATACAGAAAGGAAAAT+GGG | 0.510935 | 3.3:-85637720 | MS.gene012839:CDS |
| CCTTGAAATAGTTGCGAACA+GGG | 0.513370 | 3.3:+85637328 | None:intergenic |
| GCTGTCGGTGTTGCTCCTGT+AGG | 0.514968 | 3.3:-85634595 | MS.gene012839:CDS |
| GTTGGGTCCGGAATTGGAAG+AGG | 0.515795 | 3.3:+85641379 | None:intergenic |
| GCTGGAGCTAAACCAAGAAC+CGG | 0.519479 | 3.3:+85637381 | None:intergenic |
| ATGAGTACGCTAATGTGGAT+TGG | 0.520629 | 3.3:-85641196 | MS.gene012839:CDS |
| TCCGGAATTGGAAGAGGGGA+GGG | 0.522473 | 3.3:+85641385 | None:intergenic |
| TACGTTACAAAATGTAGTGC+TGG | 0.523205 | 3.3:-85641134 | MS.gene012839:CDS |
| CTGTCGGTGTTGCTCCTGTA+GGG | 0.530130 | 3.3:-85634594 | MS.gene012839:CDS |
| TTCTGTACAGGAACTGCTGT+CGG | 0.530561 | 3.3:-85634610 | MS.gene012839:CDS |
| GGTAGTATTGAAGATAAGAA+GGG | 0.530796 | 3.3:-85634165 | MS.gene012839:CDS |
| AACCATTTCCAATTATGCAC+CGG | 0.531718 | 3.3:-85637131 | MS.gene012839:intron |
| AGGAATGTGTATTCAGGTGC+TGG | 0.532284 | 3.3:+85637363 | None:intergenic |
| TGGTTATCGTTCTTCCTCCA+AGG | 0.534170 | 3.3:-85642867 | MS.gene012839:intron |
| AAAATATAGGGATTATTCGA+AGG | 0.536039 | 3.3:-85637748 | MS.gene012839:intron |
| GTAACGTACATGAAATCGGT+TGG | 0.536355 | 3.3:+85641149 | None:intergenic |
| ATTGAACTTAGCCCATCCGC+AGG | 0.539047 | 3.3:-85641071 | MS.gene012839:CDS |
| TGAGTACGCTAATGTGGATT+GGG | 0.540805 | 3.3:-85641195 | MS.gene012839:CDS |
| TCTTCCACGTACAAATTAAG+TGG | 0.543543 | 3.3:+85637196 | None:intergenic |
| TAATTTAGGACCCCTGCGGA+TGG | 0.548755 | 3.3:+85641059 | None:intergenic |
| TAGGTTCCTCCACCGGGCAA+AGG | 0.559583 | 3.3:-85637441 | MS.gene012839:CDS |
| AGGGGTCCTAAATTATGGCC+AGG | 0.559751 | 3.3:-85641051 | MS.gene012839:intron |
| ATGGACAGCTTAATCGGTAT+GGG | 0.560555 | 3.3:-85641097 | MS.gene012839:CDS |
| TACCTGATAACCCAAATCAC+TGG | 0.560844 | 3.3:+85635977 | None:intergenic |
| CAGTCCCGCGTTGCGAAGCA+CGG | 0.561077 | 3.3:+85637164 | None:intergenic |
| TTGACCGTGCTTCGCAACGC+GGG | 0.561096 | 3.3:-85637168 | MS.gene012839:CDS |
| TGGTATGTGATACTCCCTAC+AGG | 0.561876 | 3.3:+85634580 | None:intergenic |
| CGATGATGAGTACGCTAATG+TGG | 0.569452 | 3.3:-85641201 | MS.gene012839:CDS |
| CAGCTCTAGCAAATAAACGT+TGG | 0.570115 | 3.3:-85637585 | MS.gene012839:CDS |
| AATGTATAGGTTCCTCCACC+GGG | 0.570735 | 3.3:-85637447 | MS.gene012839:intron |
| TTGGGTCCGGAATTGGAAGA+GGG | 0.575596 | 3.3:+85641380 | None:intergenic |
| AGTATGGTTCCATTGATAGC+AGG | 0.575714 | 3.3:+85636035 | None:intergenic |
| GAACAGGGGAAGCATATATG+AGG | 0.580432 | 3.3:+85637343 | None:intergenic |
| TAAAACGTTGATCACCTTGG+AGG | 0.582579 | 3.3:+85642853 | None:intergenic |
| ACAGAAAACTTCATCTGCCT+CGG | 0.585007 | 3.3:+85634627 | None:intergenic |
| AATTTAGGACCCCTGCGGAT+GGG | 0.586287 | 3.3:+85641060 | None:intergenic |
| GGAAGAGCGCGTTGTTGCTG+TGG | 0.591202 | 3.3:-85634659 | MS.gene012839:CDS |
| TTGGAGGAAGAACGATAACC+AGG | 0.591557 | 3.3:+85642869 | None:intergenic |
| CGCGCCACTTAATTTGTACG+TGG | 0.594998 | 3.3:-85637200 | MS.gene012839:CDS |
| ACTGCTCTTAGGAAGTGGTC+CGG | 0.600004 | 3.3:-85637400 | MS.gene012839:CDS |
| TTTGACCGTGCTTCGCAACG+CGG | 0.611947 | 3.3:-85637169 | MS.gene012839:CDS |
| TCAACAAATTGATCAATGGA+AGG | 0.615530 | 3.3:+85637622 | None:intergenic |
| GTATTGAAGATAAGAAGGGA+TGG | 0.616637 | 3.3:-85634161 | MS.gene012839:CDS |
| TTGAACTTAGCCCATCCGCA+GGG | 0.617818 | 3.3:-85641070 | MS.gene012839:CDS |
| CAAAATAGAGCCAAGAGCAG+AGG | 0.632269 | 3.3:-85636688 | MS.gene012839:CDS |
| TTGGCATGAGTCCAAACCCA+AGG | 0.634099 | 3.3:+85641168 | None:intergenic |
| CACGTACAAATTAAGTGGCG+CGG | 0.649503 | 3.3:+85637201 | None:intergenic |
| AGGCACGAAAGCATACAGAA+AGG | 0.658001 | 3.3:-85637728 | MS.gene012839:CDS |
| TACAACGATCCTTTGCCCGG+TGG | 0.674996 | 3.3:+85637432 | None:intergenic |
| TAACAGATAGGGGCTTACAA+TGG | 0.677239 | 3.3:-85641421 | MS.gene012839:intron |
| TGAACTTAGCCCATCCGCAG+GGG | 0.684106 | 3.3:-85641069 | MS.gene012839:CDS |
| GGACCACTTCCTAAGAGCAG+TGG | 0.684872 | 3.3:+85637402 | None:intergenic |
| CTTGAAATAGTTGCGAACAG+GGG | 0.708149 | 3.3:+85637329 | None:intergenic |
| TGGGTCCGGAATTGGAAGAG+GGG | 0.711513 | 3.3:+85641381 | None:intergenic |
| TGCTGTGGATGAATTGACCG+AGG | 0.715955 | 3.3:-85634644 | MS.gene012839:CDS |
| GCCATAATTTAGGACCCCTG+CGG | 0.723216 | 3.3:+85641055 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 85634143 | 85642942 | 85634143 | ID=MS.gene012839 |
| chr3.3 | mRNA | 85634143 | 85642942 | 85634143 | ID=MS.gene012839.t1;Parent=MS.gene012839 |
| chr3.3 | exon | 85642868 | 85642942 | 85642868 | ID=MS.gene012839.t1.exon1;Parent=MS.gene012839.t1 |
| chr3.3 | CDS | 85642868 | 85642942 | 85642868 | ID=cds.MS.gene012839.t1;Parent=MS.gene012839.t1 |
| chr3.3 | exon | 85641373 | 85641437 | 85641373 | ID=MS.gene012839.t1.exon2;Parent=MS.gene012839.t1 |
| chr3.3 | CDS | 85641373 | 85641437 | 85641373 | ID=cds.MS.gene012839.t1;Parent=MS.gene012839.t1 |
| chr3.3 | exon | 85641052 | 85641226 | 85641052 | ID=MS.gene012839.t1.exon3;Parent=MS.gene012839.t1 |
| chr3.3 | CDS | 85641052 | 85641226 | 85641052 | ID=cds.MS.gene012839.t1;Parent=MS.gene012839.t1 |
| chr3.3 | exon | 85637585 | 85637761 | 85637585 | ID=MS.gene012839.t1.exon4;Parent=MS.gene012839.t1 |
| chr3.3 | CDS | 85637585 | 85637761 | 85637585 | ID=cds.MS.gene012839.t1;Parent=MS.gene012839.t1 |
| chr3.3 | exon | 85637329 | 85637460 | 85637329 | ID=MS.gene012839.t1.exon5;Parent=MS.gene012839.t1 |
| chr3.3 | CDS | 85637329 | 85637460 | 85637329 | ID=cds.MS.gene012839.t1;Parent=MS.gene012839.t1 |
| chr3.3 | exon | 85637132 | 85637230 | 85637132 | ID=MS.gene012839.t1.exon6;Parent=MS.gene012839.t1 |
| chr3.3 | CDS | 85637132 | 85637230 | 85637132 | ID=cds.MS.gene012839.t1;Parent=MS.gene012839.t1 |
| chr3.3 | exon | 85636606 | 85636722 | 85636606 | ID=MS.gene012839.t1.exon7;Parent=MS.gene012839.t1 |
| chr3.3 | CDS | 85636606 | 85636722 | 85636606 | ID=cds.MS.gene012839.t1;Parent=MS.gene012839.t1 |
| chr3.3 | exon | 85635980 | 85636075 | 85635980 | ID=MS.gene012839.t1.exon8;Parent=MS.gene012839.t1 |
| chr3.3 | CDS | 85635980 | 85636075 | 85635980 | ID=cds.MS.gene012839.t1;Parent=MS.gene012839.t1 |
| chr3.3 | exon | 85634571 | 85634683 | 85634571 | ID=MS.gene012839.t1.exon9;Parent=MS.gene012839.t1 |
| chr3.3 | CDS | 85634571 | 85634683 | 85634571 | ID=cds.MS.gene012839.t1;Parent=MS.gene012839.t1 |
| chr3.3 | exon | 85634143 | 85634257 | 85634143 | ID=MS.gene012839.t1.exon10;Parent=MS.gene012839.t1 |
| chr3.3 | CDS | 85634143 | 85634257 | 85634143 | ID=cds.MS.gene012839.t1;Parent=MS.gene012839.t1 |
| Gene Sequence |
| Protein sequence |
