Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015044.t1 | XP_013461828.1 | 97.9 | 387 | 8 | 0 | 1 | 387 | 1 | 387 | 3.40E-218 | 767.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015044.t1 | Q9M439 | 66.8 | 382 | 123 | 2 | 10 | 387 | 7 | 388 | 1.5e-145 | 517.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015044.t1 | A0A072V3G0 | 97.9 | 387 | 8 | 0 | 1 | 387 | 1 | 387 | 2.4e-218 | 767.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene015044 | MS.gene09632 | PPI |
| MS.gene015044 | MS.gene21440 | PPI |
| MS.gene015044 | MS.gene015287 | PPI |
| MS.gene015044 | MS.gene43507 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015044.t1 | MTR_3g103580 | 97.933 | 387 | 8 | 0 | 1 | 387 | 1 | 387 | 0.0 | 782 |
| MS.gene015044.t1 | MTR_3g103580 | 98.000 | 350 | 7 | 0 | 1 | 350 | 1 | 350 | 0.0 | 705 |
| MS.gene015044.t1 | MTR_3g103580 | 98.762 | 323 | 4 | 0 | 65 | 387 | 1 | 323 | 0.0 | 659 |
| MS.gene015044.t1 | MTR_5g014960 | 65.882 | 340 | 116 | 0 | 47 | 386 | 68 | 407 | 2.98e-167 | 474 |
| MS.gene015044.t1 | MTR_4g092590 | 60.294 | 340 | 131 | 2 | 50 | 387 | 20 | 357 | 1.22e-150 | 430 |
| MS.gene015044.t1 | MTR_4g092580 | 57.440 | 336 | 143 | 0 | 52 | 387 | 22 | 357 | 1.48e-146 | 419 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015044.t1 | AT1G10070 | 66.754 | 382 | 123 | 2 | 10 | 387 | 7 | 388 | 0.0 | 528 |
| MS.gene015044.t1 | AT1G10070 | 66.754 | 382 | 123 | 2 | 10 | 387 | 7 | 388 | 0.0 | 528 |
| MS.gene015044.t1 | AT1G10070 | 72.013 | 318 | 89 | 0 | 70 | 387 | 1 | 318 | 2.91e-173 | 486 |
| MS.gene015044.t1 | AT1G10060 | 61.198 | 384 | 140 | 3 | 10 | 386 | 2 | 383 | 1.09e-171 | 484 |
| MS.gene015044.t1 | AT1G10060 | 60.204 | 392 | 146 | 4 | 1 | 385 | 90 | 478 | 2.97e-170 | 484 |
| MS.gene015044.t1 | AT3G49680 | 65.385 | 338 | 115 | 2 | 51 | 386 | 73 | 410 | 7.30e-162 | 460 |
| MS.gene015044.t1 | AT3G49680 | 65.385 | 338 | 115 | 2 | 51 | 386 | 75 | 412 | 8.36e-162 | 460 |
| MS.gene015044.t1 | AT5G65780 | 63.636 | 341 | 123 | 1 | 47 | 386 | 74 | 414 | 1.56e-161 | 460 |
| MS.gene015044.t1 | AT1G10060 | 67.192 | 317 | 104 | 0 | 70 | 386 | 1 | 317 | 1.37e-158 | 449 |
| MS.gene015044.t1 | AT1G10060 | 62.931 | 348 | 120 | 3 | 10 | 350 | 2 | 347 | 8.52e-156 | 444 |
| MS.gene015044.t1 | AT1G10060 | 61.625 | 357 | 127 | 4 | 1 | 350 | 90 | 443 | 1.14e-154 | 444 |
| MS.gene015044.t1 | AT1G50090 | 58.192 | 354 | 145 | 2 | 36 | 387 | 3 | 355 | 1.21e-153 | 438 |
| MS.gene015044.t1 | AT1G50110 | 56.818 | 352 | 150 | 2 | 36 | 387 | 3 | 352 | 8.56e-152 | 433 |
| MS.gene015044.t1 | AT1G50110 | 57.862 | 318 | 133 | 1 | 70 | 387 | 1 | 317 | 3.78e-140 | 402 |
| MS.gene015044.t1 | AT3G19710 | 50.427 | 351 | 173 | 1 | 37 | 387 | 2 | 351 | 1.15e-134 | 389 |
| MS.gene015044.t1 | AT1G10060 | 68.651 | 252 | 79 | 0 | 48 | 299 | 45 | 296 | 4.88e-127 | 368 |
| MS.gene015044.t1 | AT1G10060 | 59.477 | 306 | 114 | 4 | 1 | 299 | 90 | 392 | 8.33e-126 | 369 |
Find 114 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGTGAACTTTCCAAGTTTA+AGG | 0.116340 | 3.2:-85990505 | MS.gene015044:CDS |
| TGTAGTGCTGGCCAAAATTT+TGG | 0.137189 | 3.2:-85988721 | MS.gene015044:CDS |
| CATCACTTTACCTTCTATTC+TGG | 0.200728 | 3.2:+85983374 | None:intergenic |
| AAATGGTACCGGTGCATAAT+TGG | 0.204590 | 3.2:+85985801 | None:intergenic |
| TTAGGAAGTGGTCCGGTTCT+TGG | 0.250877 | 3.2:-85986091 | MS.gene015044:CDS |
| ACCTGTTTGTATTCCTAAAA+TGG | 0.255480 | 3.2:+85982999 | None:intergenic |
| TGCCAGTGATTTGGGTTATC+AGG | 0.275271 | 3.2:-85984613 | MS.gene015044:intron |
| GCTGTCCATGTCCAAAATTT+TGG | 0.281068 | 3.2:+85988710 | None:intergenic |
| ATCATACCTGGCCATAATTT+AGG | 0.288384 | 3.2:+85988644 | None:intergenic |
| CAATTCCGGACCCAACTTAC+AGG | 0.297018 | 3.2:-85988970 | MS.gene015044:intron |
| AAAGCATACAGAAAGGAAAA+TGG | 0.301781 | 3.2:-85986421 | MS.gene015044:CDS |
| CATTGAAATTGCCAGTGATT+TGG | 0.302811 | 3.2:-85984622 | MS.gene015044:CDS |
| TCCGCAGGGGTCCTAAATTA+TGG | 0.310746 | 3.2:-85988655 | MS.gene015044:CDS |
| GCAAAGGGTCGTTGTATCTT+AGG | 0.318014 | 3.2:-85986123 | MS.gene015044:CDS |
| GATTGGGACAGCCTTGGGTT+TGG | 0.329076 | 3.2:-85988778 | MS.gene015044:CDS |
| GCCCTCCCCTCTTCCAATTC+CGG | 0.331887 | 3.2:-85988984 | MS.gene015044:CDS |
| CTGTAAGTTGGGTCCGGAAT+TGG | 0.338583 | 3.2:+85988971 | None:intergenic |
| CCCTGTTCGCAACTATTTCA+AGG | 0.338859 | 3.2:-85986026 | MS.gene015044:intron |
| TCTTGTGATTCCGGAAAGTA+TGG | 0.340136 | 3.2:+85984653 | None:intergenic |
| GGAAAGTATGGTTCCATTGA+TGG | 0.362249 | 3.2:+85984665 | None:intergenic |
| ATGTGGATTGGGACAGCCTT+GGG | 0.366715 | 3.2:-85988783 | MS.gene015044:CDS |
| CCTTGACATGTAGGTCTTGA+TGG | 0.367499 | 3.2:-85985359 | MS.gene015044:intron |
| TATCTTAGGCCACTGCTCTT+AGG | 0.375755 | 3.2:-85986109 | MS.gene015044:CDS |
| ATTGAAATTGCCAGTGATTT+GGG | 0.377285 | 3.2:-85984621 | MS.gene015044:CDS |
| TTGGACATGGACAGCTTAAT+CGG | 0.380425 | 3.2:-85988702 | MS.gene015044:CDS |
| ACCTTGAAATAGTTGCGAAC+AGG | 0.382294 | 3.2:+85986025 | None:intergenic |
| TGCTAGAGCAGTTTGCTTCA+AGG | 0.383554 | 3.2:+85986297 | None:intergenic |
| TATATGAGGAATGTGTATTC+AGG | 0.397711 | 3.2:+85986055 | None:intergenic |
| AGAAGCAGTTTCCTTAAACT+TGG | 0.398954 | 3.2:+85990494 | None:intergenic |
| AAGTGGTCCGGTTCTTGGTT+TGG | 0.404166 | 3.2:-85986086 | MS.gene015044:CDS |
| GTGGACTATAAAACAGGTTC+TGG | 0.405793 | 3.2:-85983048 | MS.gene015044:CDS |
| TATTATAGTTACCTGTAAGT+TGG | 0.436071 | 3.2:+85988959 | None:intergenic |
| CGTGCTTCGCAACGCGGGAC+TGG | 0.439406 | 3.2:-85985841 | MS.gene015044:CDS |
| ATTATAGTTACCTGTAAGTT+GGG | 0.439858 | 3.2:+85988960 | None:intergenic |
| ATTGATGGCAGGCGTTGAAA+TGG | 0.440858 | 3.2:+85984680 | None:intergenic |
| AGGTAGTATTGAAGATAAGA+AGG | 0.442292 | 3.2:-85982980 | MS.gene015044:CDS |
| ATCAATGGAACCATACTTTC+CGG | 0.443211 | 3.2:-85984663 | MS.gene015044:CDS |
| CTTGCACAACTCTTGACAAA+TGG | 0.449586 | 3.2:+85983023 | None:intergenic |
| TACCGGTGCATAATTGGAAA+TGG | 0.455649 | 3.2:+85985807 | None:intergenic |
| CATGGACAGCTTAATCGGTA+TGG | 0.457574 | 3.2:-85988697 | MS.gene015044:CDS |
| ACTTAATTTGTATGTGGAAG+AGG | 0.457846 | 3.2:-85985872 | MS.gene015044:CDS |
| ATTTCAACGCCTGCCATCAA+TGG | 0.460138 | 3.2:-85984678 | MS.gene015044:CDS |
| GTATCACATACCAGAATAGA+AGG | 0.460502 | 3.2:-85983384 | MS.gene015044:intron |
| TAATTTATAGGTTCCTCCAC+CGG | 0.461428 | 3.2:-85986146 | MS.gene015044:intron |
| AGGAAACTGCTTCTTCGTCC+TGG | 0.467038 | 3.2:-85990485 | MS.gene015044:CDS |
| TGCAGGGTGGACTATAAAAC+AGG | 0.473120 | 3.2:-85983054 | MS.gene015044:intron |
| ATCGGAAAATCCTCTGCTCT+TGG | 0.477180 | 3.2:+85985324 | None:intergenic |
| AATGTGGATTGGGACAGCCT+TGG | 0.478960 | 3.2:-85988784 | MS.gene015044:CDS |
| GTCCGGAATTGGAAGAGGGG+AGG | 0.483252 | 3.2:+85988982 | None:intergenic |
| AATGCAATTCGCATGAAGAT+TGG | 0.484572 | 3.2:-85986370 | MS.gene015044:CDS |
| CGCGCCACTTAATTTGTATG+TGG | 0.486258 | 3.2:-85985878 | MS.gene015044:CDS |
| GGCATCAACAAATTGATCAA+TGG | 0.490126 | 3.2:+85986318 | None:intergenic |
| GCTGGAGCCAAACCAAGAAC+CGG | 0.491499 | 3.2:+85986079 | None:intergenic |
| AGTTACCTGTAAGTTGGGTC+CGG | 0.496236 | 3.2:+85988965 | None:intergenic |
| TGCTCTAGCAAACAAGCGTT+GGG | 0.496963 | 3.2:-85986284 | MS.gene015044:intron |
| AGGTTCCTCCACCGGGCAAA+GGG | 0.499399 | 3.2:-85986138 | MS.gene015044:CDS |
| AGATACAACGACCCTTTGCC+CGG | 0.499668 | 3.2:+85986127 | None:intergenic |
| TAGGTTCCTCCACCGGGCAA+AGG | 0.501342 | 3.2:-85986139 | MS.gene015044:CDS |
| CTGCTCTAGCAAACAAGCGT+TGG | 0.505553 | 3.2:-85986285 | MS.gene015044:CDS |
| TGATTACGCTAATGTGGATT+GGG | 0.508424 | 3.2:-85988794 | MS.gene015044:CDS |
| AGGCCACTGCTCTTAGGAAG+TGG | 0.509303 | 3.2:-85986103 | MS.gene015044:CDS |
| ATGATTACGCTAATGTGGAT+TGG | 0.509628 | 3.2:-85988795 | MS.gene015044:CDS |
| AAGCATACAGAAAGGAAAAT+GGG | 0.510935 | 3.2:-85986420 | MS.gene015044:CDS |
| CCTTGAAATAGTTGCGAACA+GGG | 0.513370 | 3.2:+85986026 | None:intergenic |
| GCTGTCGGTGTTGCTCCTGT+AGG | 0.514968 | 3.2:-85983409 | MS.gene015044:CDS |
| GTTGGGTCCGGAATTGGAAG+AGG | 0.515795 | 3.2:+85988977 | None:intergenic |
| AACGACCCTTTGCCCGGTGG+AGG | 0.518065 | 3.2:+85986133 | None:intergenic |
| TCCGGAATTGGAAGAGGGGA+GGG | 0.522473 | 3.2:+85988983 | None:intergenic |
| TACGTTACAAAATGTAGTGC+TGG | 0.523205 | 3.2:-85988733 | MS.gene015044:CDS |
| AATTTATAGGTTCCTCCACC+GGG | 0.527583 | 3.2:-85986145 | MS.gene015044:intron |
| CTGTCGGTGTTGCTCCTGTA+GGG | 0.530130 | 3.2:-85983408 | MS.gene015044:CDS |
| TTCTGTACAGGAACTGCTGT+CGG | 0.530561 | 3.2:-85983424 | MS.gene015044:CDS |
| GGTAGTATTGAAGATAAGAA+GGG | 0.530796 | 3.2:-85982979 | MS.gene015044:CDS |
| AACCATTTCCAATTATGCAC+CGG | 0.531718 | 3.2:-85985809 | MS.gene015044:intron |
| AGGAATGTGTATTCAGGTGC+TGG | 0.532284 | 3.2:+85986061 | None:intergenic |
| TCTTCCACATACAAATTAAG+TGG | 0.533831 | 3.2:+85985874 | None:intergenic |
| TGGTTATCGTTCTTCCTCCA+AGG | 0.534170 | 3.2:-85990465 | MS.gene015044:intron |
| GTAACGTACATGAAATCGGT+TGG | 0.536355 | 3.2:+85988748 | None:intergenic |
| ATTGAACTTAGCCCATCCGC+AGG | 0.539047 | 3.2:-85988670 | MS.gene015044:CDS |
| AATATATAGGGATTATTCGA+AGG | 0.543618 | 3.2:-85986448 | MS.gene015044:intron |
| TAATTTAGGACCCCTGCGGA+TGG | 0.548755 | 3.2:+85988658 | None:intergenic |
| AGGGGTCCTAAATTATGGCC+AGG | 0.559751 | 3.2:-85988650 | MS.gene015044:intron |
| ATGGACAGCTTAATCGGTAT+GGG | 0.560555 | 3.2:-85988696 | MS.gene015044:CDS |
| TACCTGATAACCCAAATCAC+TGG | 0.560844 | 3.2:+85984611 | None:intergenic |
| CAGTCCCGCGTTGCGAAGCA+CGG | 0.561077 | 3.2:+85985842 | None:intergenic |
| TTGACCGTGCTTCGCAACGC+GGG | 0.561096 | 3.2:-85985846 | MS.gene015044:CDS |
| TGGTATGTGATACTCCCTAC+AGG | 0.561876 | 3.2:+85983394 | None:intergenic |
| TTGGGTCCGGAATTGGAAGA+GGG | 0.575596 | 3.2:+85988978 | None:intergenic |
| GAACAGGGGAAGCATATATG+AGG | 0.580432 | 3.2:+85986041 | None:intergenic |
| AGTATGGTTCCATTGATGGC+AGG | 0.580853 | 3.2:+85984669 | None:intergenic |
| CGATGATGATTACGCTAATG+TGG | 0.581764 | 3.2:-85988800 | MS.gene015044:CDS |
| TAAAACGTTGATCACCTTGG+AGG | 0.582579 | 3.2:+85990451 | None:intergenic |
| ACAGAAAACTTCATCTGCCT+CGG | 0.585007 | 3.2:+85983441 | None:intergenic |
| AATTTAGGACCCCTGCGGAT+GGG | 0.586287 | 3.2:+85988659 | None:intergenic |
| GGAAGAGCGCGTTGTTGCTG+TGG | 0.591202 | 3.2:-85983473 | MS.gene015044:CDS |
| TTGGAGGAAGAACGATAACC+AGG | 0.591557 | 3.2:+85990467 | None:intergenic |
| ACTGCTCTTAGGAAGTGGTC+CGG | 0.600004 | 3.2:-85986098 | MS.gene015044:CDS |
| TCAACAAATTGATCAATGGA+AGG | 0.611879 | 3.2:+85986322 | None:intergenic |
| TTTGACCGTGCTTCGCAACG+CGG | 0.611947 | 3.2:-85985847 | MS.gene015044:CDS |
| GTATTGAAGATAAGAAGGGA+TGG | 0.616637 | 3.2:-85982975 | MS.gene015044:CDS |
| TTGAACTTAGCCCATCCGCA+GGG | 0.617818 | 3.2:-85988669 | MS.gene015044:CDS |
| CACATACAAATTAAGTGGCG+CGG | 0.630226 | 3.2:+85985879 | None:intergenic |
| CAAAATAGAGCCAAGAGCAG+AGG | 0.632269 | 3.2:-85985334 | MS.gene015044:CDS |
| TTGGCATGAGTCCAAACCCA+AGG | 0.634099 | 3.2:+85988767 | None:intergenic |
| AGGCACGAAAGCATACAGAA+AGG | 0.658001 | 3.2:-85986428 | MS.gene015044:CDS |
| TGAATCAAGGTATAACACAT+CGG | 0.667794 | 3.2:+85985306 | None:intergenic |
| TAACAGATAGGGGCTTACAA+TGG | 0.677239 | 3.2:-85989019 | MS.gene015044:intron |
| TGAACTTAGCCCATCCGCAG+GGG | 0.684106 | 3.2:-85988668 | MS.gene015044:CDS |
| GGACCACTTCCTAAGAGCAG+TGG | 0.684872 | 3.2:+85986100 | None:intergenic |
| TACAACGACCCTTTGCCCGG+TGG | 0.687415 | 3.2:+85986130 | None:intergenic |
| CTTGAAATAGTTGCGAACAG+GGG | 0.708149 | 3.2:+85986027 | None:intergenic |
| TGGGTCCGGAATTGGAAGAG+GGG | 0.711513 | 3.2:+85988979 | None:intergenic |
| TGCTGTGGATGAATTGACCG+AGG | 0.715955 | 3.2:-85983458 | MS.gene015044:CDS |
| GCCATAATTTAGGACCCCTG+CGG | 0.723216 | 3.2:+85988654 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 85982957 | 85990540 | 85982957 | ID=MS.gene015044 |
| chr3.2 | mRNA | 85982957 | 85990540 | 85982957 | ID=MS.gene015044.t1;Parent=MS.gene015044 |
| chr3.2 | exon | 85990466 | 85990540 | 85990466 | ID=MS.gene015044.t1.exon1;Parent=MS.gene015044.t1 |
| chr3.2 | CDS | 85990466 | 85990540 | 85990466 | ID=cds.MS.gene015044.t1;Parent=MS.gene015044.t1 |
| chr3.2 | exon | 85988971 | 85989035 | 85988971 | ID=MS.gene015044.t1.exon2;Parent=MS.gene015044.t1 |
| chr3.2 | CDS | 85988971 | 85989035 | 85988971 | ID=cds.MS.gene015044.t1;Parent=MS.gene015044.t1 |
| chr3.2 | exon | 85988651 | 85988825 | 85988651 | ID=MS.gene015044.t1.exon3;Parent=MS.gene015044.t1 |
| chr3.2 | CDS | 85988651 | 85988825 | 85988651 | ID=cds.MS.gene015044.t1;Parent=MS.gene015044.t1 |
| chr3.2 | exon | 85986285 | 85986461 | 85986285 | ID=MS.gene015044.t1.exon4;Parent=MS.gene015044.t1 |
| chr3.2 | CDS | 85986285 | 85986461 | 85986285 | ID=cds.MS.gene015044.t1;Parent=MS.gene015044.t1 |
| chr3.2 | exon | 85986027 | 85986158 | 85986027 | ID=MS.gene015044.t1.exon5;Parent=MS.gene015044.t1 |
| chr3.2 | CDS | 85986027 | 85986158 | 85986027 | ID=cds.MS.gene015044.t1;Parent=MS.gene015044.t1 |
| chr3.2 | exon | 85985810 | 85985908 | 85985810 | ID=MS.gene015044.t1.exon6;Parent=MS.gene015044.t1 |
| chr3.2 | CDS | 85985810 | 85985908 | 85985810 | ID=cds.MS.gene015044.t1;Parent=MS.gene015044.t1 |
| chr3.2 | exon | 85985252 | 85985368 | 85985252 | ID=MS.gene015044.t1.exon7;Parent=MS.gene015044.t1 |
| chr3.2 | CDS | 85985252 | 85985368 | 85985252 | ID=cds.MS.gene015044.t1;Parent=MS.gene015044.t1 |
| chr3.2 | exon | 85984614 | 85984709 | 85984614 | ID=MS.gene015044.t1.exon8;Parent=MS.gene015044.t1 |
| chr3.2 | CDS | 85984614 | 85984709 | 85984614 | ID=cds.MS.gene015044.t1;Parent=MS.gene015044.t1 |
| chr3.2 | exon | 85983385 | 85983497 | 85983385 | ID=MS.gene015044.t1.exon9;Parent=MS.gene015044.t1 |
| chr3.2 | CDS | 85983385 | 85983497 | 85983385 | ID=cds.MS.gene015044.t1;Parent=MS.gene015044.t1 |
| chr3.2 | exon | 85982957 | 85983071 | 85982957 | ID=MS.gene015044.t1.exon10;Parent=MS.gene015044.t1 |
| chr3.2 | CDS | 85982957 | 85983071 | 85982957 | ID=cds.MS.gene015044.t1;Parent=MS.gene015044.t1 |
| Gene Sequence |
| Protein sequence |
