AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene016581

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016581.t1 MTR_8g039540 97.005 434 13 0 1 434 1 434 0.0 857
MS.gene016581.t1 MTR_1g037630 82.692 416 71 1 19 434 15 429 0.0 691
MS.gene016581.t1 MTR_3g014290 80.769 416 67 2 19 434 15 417 0.0 662
MS.gene016581.t1 MTR_3g100500 54.875 441 187 7 1 433 1 437 6.12e-145 420
MS.gene016581.t1 MTR_3g097150 31.618 408 239 15 51 433 54 446 1.59e-53 185
MS.gene016581.t1 MTR_7g117430 31.915 423 261 11 28 433 72 484 9.96e-49 173
MS.gene016581.t1 MTR_1g111350 31.738 397 248 10 50 433 81 467 1.43e-48 172
MS.gene016581.t1 MTR_3g053710 32.468 385 226 14 66 428 89 461 1.52e-48 172
MS.gene016581.t1 MTR_1g115210 35.244 349 211 9 94 433 144 486 2.18e-48 172
MS.gene016581.t1 MTR_2g028190 32.346 439 237 19 35 433 33 451 4.47e-48 171
MS.gene016581.t1 MTR_4g007740 31.481 378 214 14 83 434 120 478 5.77e-45 163
MS.gene016581.t1 MTR_6g085010 32.948 346 212 10 97 433 157 491 3.95e-41 152
MS.gene016581.t1 MTR_8g103880 29.107 347 227 10 97 433 117 454 6.65e-41 151
MS.gene016581.t1 MTR_3g046300 32.061 393 230 12 71 433 73 458 9.81e-41 151
MS.gene016581.t1 MTR_5g091130 32.570 393 224 15 71 433 172 553 5.76e-40 150
MS.gene016581.t1 MTR_1g039270 33.048 351 217 9 94 433 126 469 6.59e-40 149
MS.gene016581.t1 MTR_4g100990 31.421 401 206 16 97 433 75 470 1.00e-38 146
MS.gene016581.t1 MTR_3g053980 33.516 364 211 15 83 428 130 480 2.49e-38 145
MS.gene016581.t1 MTR_0036s0110 27.570 428 270 16 32 434 29 441 6.60e-38 143
MS.gene016581.t1 MTR_0024s0040 30.969 423 255 16 35 434 1 409 6.05e-36 137
MS.gene016581.t1 MTR_5g038360 29.673 428 265 17 29 434 22 435 2.76e-34 133
MS.gene016581.t1 MTR_5g008110 31.486 397 226 19 72 434 30 414 6.32e-34 131
MS.gene016581.t1 MTR_5g012490 28.736 435 230 18 65 433 49 469 9.54e-34 132
MS.gene016581.t1 MTR_3g065160 29.477 363 219 15 98 434 76 427 6.33e-33 129
MS.gene016581.t1 MTR_7g062360 27.763 371 225 12 99 433 62 425 1.58e-32 128
MS.gene016581.t1 MTR_2g059460 28.261 368 228 16 92 434 49 405 2.55e-32 127
MS.gene016581.t1 MTR_8g096990 26.914 431 271 16 10 421 12 417 4.50e-32 127
MS.gene016581.t1 MTR_3g028120 27.378 431 272 16 29 434 23 437 1.07e-31 125
MS.gene016581.t1 MTR_5g077850 28.493 365 224 13 98 434 83 438 4.70e-31 124
MS.gene016581.t1 MTR_2g059490 27.624 362 237 14 92 434 54 409 5.91e-31 124
MS.gene016581.t1 MTR_0036s0070 26.733 404 263 13 32 421 28 412 1.35e-30 122
MS.gene016581.t1 MTR_7g105850 29.091 385 215 16 97 433 91 465 2.02e-30 122
MS.gene016581.t1 MTR_8g038280 27.494 451 273 19 10 434 12 434 8.40e-30 120
MS.gene016581.t1 MTR_5g008150 28.678 401 243 18 62 434 26 411 1.38e-29 119
MS.gene016581.t1 MTR_4g056420 27.470 415 242 20 61 434 41 437 1.87e-29 120
MS.gene016581.t1 MTR_5g080890 27.009 448 283 17 7 434 9 432 5.06e-29 118
MS.gene016581.t1 MTR_2g084550 27.027 407 254 15 61 434 42 438 3.15e-28 116
MS.gene016581.t1 MTR_5g080960 26.786 448 284 17 7 434 9 432 4.41e-28 115
MS.gene016581.t1 MTR_3g068000 26.963 382 222 15 96 433 73 441 8.59e-28 115
MS.gene016581.t1 MTR_0036s0050 26.887 424 272 16 32 434 28 434 1.27e-27 114
MS.gene016581.t1 MTR_5g016260 28.099 363 217 18 97 434 89 432 2.32e-27 115
MS.gene016581.t1 MTR_5g016260 28.099 363 217 18 97 434 89 432 2.45e-27 115
MS.gene016581.t1 MTR_8g038250 27.126 435 259 16 5 421 7 401 6.66e-27 112
MS.gene016581.t1 MTR_8g038310 27.842 431 256 18 5 421 10 399 7.91e-27 111
MS.gene016581.t1 MTR_5g077920 30.352 369 214 18 98 434 70 427 7.80e-26 109
MS.gene016581.t1 MTR_5g044770 26.879 346 226 12 92 421 82 416 1.84e-25 108
MS.gene016581.t1 MTR_5g044570 27.400 427 250 16 29 434 23 410 7.03e-25 106
MS.gene016581.t1 MTR_7g102750 27.200 375 221 18 97 434 82 441 5.28e-24 104
MS.gene016581.t1 MTR_1g060750 26.420 352 217 14 97 434 52 375 5.52e-24 103
MS.gene016581.t1 MTR_2g055840 27.446 368 228 12 97 433 14 373 8.51e-24 102
MS.gene016581.t1 MTR_2g070430 26.954 371 224 14 94 426 85 446 1.74e-23 102
MS.gene016581.t1 MTR_6g090290 27.111 450 283 18 7 434 9 435 2.40e-23 102
MS.gene016581.t1 MTR_1g061940 24.885 434 263 19 46 434 30 445 4.56e-23 101
MS.gene016581.t1 MTR_2g067830 27.500 360 227 15 97 434 58 405 7.95e-23 100
MS.gene016581.t1 MTR_5g022880 28.495 372 208 17 97 433 113 461 1.28e-22 100
MS.gene016581.t1 MTR_4g095270 26.111 360 226 16 97 433 81 423 1.29e-22 100
MS.gene016581.t1 MTR_4g095270 26.111 360 226 16 97 433 81 423 1.29e-22 100
MS.gene016581.t1 MTR_4g045857 31.982 222 130 10 89 289 45 266 3.04e-22 96.7
MS.gene016581.t1 MTR_5g008070 28.406 433 265 20 21 434 19 425 1.02e-21 97.1
MS.gene016581.t1 MTR_7g102750 29.375 320 181 16 97 382 82 390 2.42e-21 96.3
MS.gene016581.t1 MTR_7g080230 26.055 403 249 18 65 434 45 431 2.77e-21 96.3
MS.gene016581.t1 MTR_7g021510 25.806 372 232 13 97 434 73 434 3.15e-21 96.3
MS.gene016581.t1 MTR_6g088760 26.471 374 235 15 69 418 63 420 4.87e-21 95.9
MS.gene016581.t1 MTR_4g023650 27.441 379 212 17 97 433 62 419 1.79e-20 93.6
MS.gene016581.t1 MTR_5g061690 27.273 363 209 15 104 433 109 449 1.87e-19 90.9
MS.gene016581.t1 MTR_2g105300 26.888 331 208 15 118 426 57 375 3.09e-19 89.4
MS.gene016581.t1 MTR_4g109760 27.660 376 235 16 79 434 39 397 6.38e-18 85.5
MS.gene016581.t1 MTR_5g008090 28.808 302 191 12 149 434 20 313 9.52e-18 84.0
MS.gene016581.t1 MTR_3g096930 26.005 373 219 17 97 434 85 435 5.43e-17 83.2
MS.gene016581.t1 MTR_8g094590 25.296 423 255 19 41 427 21 418 6.21e-17 82.8
MS.gene016581.t1 MTR_1g078240 27.673 318 186 17 97 381 70 376 1.74e-16 81.3
MS.gene016581.t1 MTR_3g087780 25.745 369 210 18 104 433 107 450 6.50e-15 77.0
MS.gene016581.t1 MTR_8g094590 25.495 404 240 19 41 408 21 399 1.25e-14 75.9
MS.gene016581.t1 MTR_2g022450 23.430 414 238 17 65 432 34 414 4.17e-14 74.3
MS.gene016581.t1 MTR_8g094590 26.861 309 183 15 41 330 21 305 2.53e-13 70.9
MS.gene016581.t1 MTR_2g022480 22.404 366 223 15 104 420 57 410 2.61e-13 71.6
MS.gene016581.t1 MTR_1g072420 24.259 371 224 15 105 428 55 415 3.97e-13 71.2
MS.gene016581.t1 MTR_7g080230 26.852 324 200 16 137 434 49 361 5.16e-13 70.9
MS.gene016581.t1 MTR_1g072380 23.721 430 243 18 58 428 28 431 5.98e-13 70.9
MS.gene016581.t1 MTR_2g022430 24.274 379 231 16 97 428 40 409 9.58e-12 67.0
MS.gene016581.t1 MTR_1g093100 22.365 389 231 14 94 427 44 416 8.15e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016581.t1 AT5G07030 72.422 417 109 3 24 434 39 455 0.0 602
MS.gene016581.t1 AT3G54400 68.585 417 122 5 20 434 16 425 0.0 578
MS.gene016581.t1 AT1G09750 52.998 417 181 8 30 433 34 448 5.14e-143 416
MS.gene016581.t1 AT5G10770 32.867 429 247 16 33 434 60 474 7.14e-54 187
MS.gene016581.t1 AT5G10760 32.697 419 247 12 33 433 63 464 5.99e-51 179
MS.gene016581.t1 AT3G25700 33.171 410 230 14 61 433 47 449 2.07e-50 177
MS.gene016581.t1 AT2G03200 32.240 366 211 12 94 433 104 458 2.56e-49 174
MS.gene016581.t1 AT3G61820 33.989 356 215 7 94 433 132 483 7.67e-47 168
MS.gene016581.t1 AT1G01300 31.250 432 245 15 34 433 73 484 6.93e-46 166
MS.gene016581.t1 AT3G20015 30.693 404 252 9 53 433 72 470 2.73e-45 164
MS.gene016581.t1 AT1G25510 33.333 348 215 8 94 433 145 483 1.30e-43 159
MS.gene016581.t1 AT3G18490 33.056 360 222 11 84 433 150 500 2.04e-42 156
MS.gene016581.t1 AT1G79720 31.156 398 226 18 67 433 102 482 4.55e-42 155
MS.gene016581.t1 AT5G45120 30.424 401 210 17 97 433 83 478 2.39e-39 148
MS.gene016581.t1 AT1G66180 29.383 405 226 20 71 434 42 427 1.61e-37 142
MS.gene016581.t1 AT2G42980 30.311 386 232 13 80 433 144 524 5.33e-37 142
MS.gene016581.t1 AT3G52500 30.649 385 214 14 97 434 90 468 1.31e-36 140
MS.gene016581.t1 AT3G59080 28.995 438 245 14 58 433 99 532 7.47e-36 139
MS.gene016581.t1 AT5G37540 28.822 399 236 19 72 433 51 438 1.43e-33 131
MS.gene016581.t1 AT3G02740 28.607 402 243 16 62 434 54 440 1.07e-32 129
MS.gene016581.t1 AT2G39710 30.000 370 214 15 96 428 64 425 1.28e-32 128
MS.gene016581.t1 AT3G02740 29.428 367 219 16 97 434 10 365 5.22e-32 126
MS.gene016581.t1 AT5G22850 27.078 421 254 18 52 434 36 441 1.25e-30 123
MS.gene016581.t1 AT3G12700 28.708 418 246 18 51 434 62 461 1.74e-30 122
MS.gene016581.t1 AT5G02190 28.689 366 218 12 106 433 82 442 2.66e-30 122
MS.gene016581.t1 AT3G59080 28.140 430 223 14 58 433 99 496 3.33e-28 117
MS.gene016581.t1 AT2G23945 27.536 414 254 17 52 433 49 448 8.56e-28 115
MS.gene016581.t1 AT1G64830 26.761 426 270 15 31 434 24 429 7.73e-27 112
MS.gene016581.t1 AT4G30040 25.901 444 272 19 4 421 8 420 2.00e-26 110
MS.gene016581.t1 AT5G33340 26.667 345 227 11 93 420 86 421 9.24e-26 108
MS.gene016581.t1 AT4G30030 28.249 354 225 15 97 433 78 419 1.26e-25 108
MS.gene016581.t1 AT1G08210 29.087 416 250 18 52 434 38 441 1.27e-25 109
MS.gene016581.t1 AT1G08210 29.327 416 249 18 52 434 72 475 1.28e-25 109
MS.gene016581.t1 AT1G08210 29.087 416 250 18 52 434 86 489 1.37e-25 109
MS.gene016581.t1 AT5G43100 26.158 367 232 17 90 434 69 418 1.70e-25 108
MS.gene016581.t1 AT5G43100 26.158 367 232 17 90 434 69 418 1.75e-25 109
MS.gene016581.t1 AT2G36670 24.826 431 267 18 46 434 43 458 2.05e-25 108
MS.gene016581.t1 AT2G36670 24.658 438 264 19 46 434 43 463 2.34e-24 105
MS.gene016581.t1 AT2G36670 25.936 374 226 17 97 434 34 392 3.52e-24 104
MS.gene016581.t1 AT2G36670 25.936 374 226 17 97 434 34 392 3.52e-24 104
MS.gene016581.t1 AT1G08210 30.811 370 213 17 52 388 38 397 3.58e-23 102
MS.gene016581.t1 AT3G50050 26.374 364 223 17 97 434 93 437 2.78e-22 100
MS.gene016581.t1 AT2G28040 26.361 349 208 14 91 420 50 368 8.53e-22 97.1
MS.gene016581.t1 AT2G28040 26.361 349 208 14 91 420 59 377 1.10e-21 96.7
MS.gene016581.t1 AT5G36260 23.284 408 270 17 56 434 41 434 1.42e-21 97.4
MS.gene016581.t1 AT4G35880 26.796 362 197 18 97 420 107 438 2.49e-21 96.7
MS.gene016581.t1 AT4G16563 26.698 427 227 16 83 433 75 491 3.68e-20 93.2
MS.gene016581.t1 AT1G65240 24.740 384 241 16 83 434 60 427 9.02e-20 91.7
MS.gene016581.t1 AT2G28010 25.439 342 221 11 91 420 59 378 1.59e-19 90.5
MS.gene016581.t1 AT4G33490 27.568 370 209 19 97 429 60 407 1.81e-19 90.5
MS.gene016581.t1 AT2G28030 24.926 337 217 11 97 420 61 374 3.09e-19 89.4
MS.gene016581.t1 AT3G51350 27.704 379 204 19 97 433 102 452 4.89e-19 89.7
MS.gene016581.t1 AT3G51340 26.954 371 211 17 97 433 103 447 8.27e-19 89.0
MS.gene016581.t1 AT1G77480 27.660 376 210 18 97 433 67 419 2.07e-18 87.4
MS.gene016581.t1 AT1G77480 27.660 376 210 18 97 433 67 419 3.50e-18 87.0
MS.gene016581.t1 AT2G35615 26.685 371 228 15 97 434 85 444 6.85e-18 85.9
MS.gene016581.t1 AT1G31450 26.230 366 234 14 97 434 85 442 3.91e-17 83.6
MS.gene016581.t1 AT3G51360 25.124 402 250 15 62 433 65 445 4.47e-17 83.6
MS.gene016581.t1 AT3G51360 25.124 402 250 15 62 433 55 435 6.39e-17 83.2
MS.gene016581.t1 AT2G28225 27.195 353 204 17 97 426 67 389 6.57e-17 82.8
MS.gene016581.t1 AT3G51360 25.410 366 225 13 97 433 5 351 1.56e-16 81.6
MS.gene016581.t1 AT3G51330 27.855 359 192 19 104 421 109 441 2.83e-16 80.9
MS.gene016581.t1 AT4G33490 29.811 265 145 15 97 341 57 300 1.19e-15 79.0
MS.gene016581.t1 AT2G17760 24.682 393 231 17 80 433 83 449 7.73e-15 77.0
MS.gene016581.t1 AT3G25700 31.818 176 96 7 61 215 47 219 1.25e-14 75.5
MS.gene016581.t1 AT3G25700 37.594 133 71 4 309 433 219 347 7.61e-14 72.8
MS.gene016581.t1 AT3G51340 28.082 292 156 14 97 356 103 372 3.31e-14 74.3
MS.gene016581.t1 AT3G51340 28.082 292 156 14 97 356 103 372 3.56e-14 74.7
MS.gene016581.t1 AT3G51360 27.517 298 171 13 62 335 65 341 1.15e-13 72.4
MS.gene016581.t1 AT1G44130 27.177 379 208 20 97 434 49 400 1.46e-13 72.4
MS.gene016581.t1 AT3G42550 22.113 407 257 13 46 434 26 390 1.40e-12 69.7

Find 135 sgRNAs with CRISPR-Local

Find 251 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCTTGGATGTCACATTT+TGG 0.163682 8.1:+64404942 None:intergenic
ATGTGGACTTGTAGAGATTT+TGG 0.241963 8.1:+64402588 None:intergenic
CTAAAACTTGTGGACTTAAC+AGG 0.288816 8.1:+64404597 None:intergenic
GTTATCGTTGGTACAACTAT+TGG 0.296291 8.1:+64401255 None:intergenic
GCTGCTTGGATTCCTTGTTC+TGG 0.307297 8.1:-64404654 MS.gene016581:CDS
TAATAGAGTGATGATCTTCT+AGG 0.307781 8.1:+64402458 None:intergenic
TCCACCTCAAACTTTGCTTT+TGG 0.311445 8.1:-64404697 MS.gene016581:CDS
TTGGGGTTTGAGCTTGGACT+TGG 0.319192 8.1:+64404961 None:intergenic
CCAAAAGTGTACCTTGGAAT+TGG 0.323950 8.1:+64402698 None:intergenic
TTTCTTACGGCGGTGTATAC+AGG 0.325812 8.1:+64401351 None:intergenic
TACATTGTTAAGGCTAAGTT+TGG 0.332559 8.1:-64404723 MS.gene016581:CDS
TGTGCATTCAACTTCACTTA+TGG 0.334737 8.1:-64402776 MS.gene016581:CDS
TTTAAGTCGCCTAACTTTAG+TGG 0.335521 8.1:-64402545 MS.gene016581:CDS
AGGGTTGTCACAGGTAGTTT+TGG 0.343997 8.1:+64401306 None:intergenic
CAACACCGAGTTCACGTTAT+CGG 0.358619 8.1:+64401134 None:intergenic
GCACCACAACAGGGTCTATT+GGG 0.360076 8.1:-64402647 MS.gene016581:CDS
CCGAGGCCCATTGTCGCTCT+TGG 0.369063 8.1:-64402618 MS.gene016581:CDS
GAGAACATTGGCTGAAGATA+TGG 0.376954 8.1:+64404898 None:intergenic
AACACCGAGTTCACGTTATC+GGG 0.382498 8.1:+64401135 None:intergenic
TTATCGTTGGTACAACTATT+GGG 0.382986 8.1:+64401256 None:intergenic
AGCACCACAACAGGGTCTAT+TGG 0.384446 8.1:-64402648 MS.gene016581:CDS
CGCCGGTAGCACCACATGTT+TGG 0.392098 8.1:-64401172 MS.gene016581:CDS
GGTCTATTGGGCTTGGGCCG+AGG 0.393741 8.1:-64402635 MS.gene016581:CDS
TGGGGTATACTTGATCCTCT+TGG 0.395670 8.1:+64402493 None:intergenic
CCAATTCCAAGGTACACTTT+TGG 0.404889 8.1:-64402698 MS.gene016581:CDS
GATGTGCCCAACTCAAGAAT+TGG 0.410152 8.1:-64401066 MS.gene016581:CDS
GGCTGTGTTAACAAAACAAC+AGG 0.414272 8.1:-64402677 MS.gene016581:CDS
TCAGTTGTACCAATTGCATC+AGG 0.418514 8.1:-64404771 MS.gene016581:CDS
CGATATCCCTCCGGCTGCGC+TGG 0.418711 8.1:-64402396 MS.gene016581:CDS
GGGGTATACTTGATCCTCTT+GGG 0.424317 8.1:+64402494 None:intergenic
AGGAAGTTCAGCACCACAAC+AGG 0.431654 8.1:-64402657 MS.gene016581:CDS
TTTAGTGGGTCGTTGAGACT+TGG 0.434229 8.1:-64402530 MS.gene016581:CDS
TTCAACCCAACTACCGGTGC+TGG 0.436030 8.1:-64402371 MS.gene016581:CDS
CTTTGGGCCAAGAGCGACAA+TGG 0.436884 8.1:+64402611 None:intergenic
AACTTGGTTGCTATTAAAGT+TGG 0.437202 8.1:-64402431 MS.gene016581:CDS
CTTCCACCGCAAGTGGGGTT+GGG 0.437902 8.1:+64402803 None:intergenic
ATGGTTCCAGCACCGGTAGT+TGG 0.439867 8.1:+64402365 None:intergenic
GGGTTGAACGCCAGCGCAGC+CGG 0.450344 8.1:+64402386 None:intergenic
TGGTTCCAGCACCGGTAGTT+GGG 0.453469 8.1:+64402366 None:intergenic
GGCCAAACATGTGGTGCTAC+CGG 0.458811 8.1:+64401170 None:intergenic
TGGGGTTTGAGCTTGGACTT+GGG 0.460621 8.1:+64404962 None:intergenic
ATCACTCTATTATGTTAACT+TGG 0.460738 8.1:-64402447 MS.gene016581:CDS
CAAAAGTGTACCTTGGAATT+GGG 0.463126 8.1:+64402699 None:intergenic
ATGATGAGGCAGAGAAGGAA+AGG 0.465733 8.1:+64404987 None:intergenic
CTTCTAGGGTTTCTGAGAAG+TGG 0.468084 8.1:+64402473 None:intergenic
GCTTCCACCGCAAGTGGGGT+TGG 0.468113 8.1:+64402802 None:intergenic
TGAGGATGTTGTCTGGTGGT+AGG 0.470698 8.1:+64401205 None:intergenic
AACAATACCAATTCTTGAGT+TGG 0.471847 8.1:+64401059 None:intergenic
ACCACATGTTTGGCCATGGC+AGG 0.475549 8.1:-64401162 MS.gene016581:CDS
TTCTAGGGTTTCTGAGAAGT+GGG 0.476620 8.1:+64402474 None:intergenic
ACAATACCAATTCTTGAGTT+GGG 0.478056 8.1:+64401060 None:intergenic
GTGGACTTAACAGGAGCAAA+AGG 0.478097 8.1:+64404606 None:intergenic
CCAAGAGCGACAATGGGCCT+CGG 0.482750 8.1:+64402618 None:intergenic
ATTTGTCTCCCTGATGCAAT+TGG 0.484764 8.1:+64404762 None:intergenic
ATCCTCTTGGGCTGATACAC+CGG 0.486552 8.1:+64402506 None:intergenic
TTGGAATTGGGTCAGTGGCT+AGG 0.489050 8.1:+64402711 None:intergenic
ACTTGTCCAGCTTAGTTGCT+AGG 0.489142 8.1:-64404797 MS.gene016581:CDS
TGGCTAGGGTAAGTGTGTCT+TGG 0.489193 8.1:+64402726 None:intergenic
ACGATAACATTCTTGTTCTC+CGG 0.489874 8.1:-64401240 MS.gene016581:CDS
TGGAATTGGGTCAGTGGCTA+GGG 0.491060 8.1:+64402712 None:intergenic
ACAACAGGGTCTATTGGGCT+TGG 0.499921 8.1:-64402642 MS.gene016581:CDS
CAACAGGGTCTATTGGGCTT+GGG 0.502732 8.1:-64402641 MS.gene016581:CDS
AACCATCAAAACCAATGTCA+TGG 0.503013 8.1:-64404869 MS.gene016581:CDS
TTGGACCACACTAGCTGCTA+TGG 0.505139 8.1:+64402745 None:intergenic
CTCAACGACCCACTAAAGTT+AGG 0.505593 8.1:+64402536 None:intergenic
ACAAGTATTGCATTCTAGCT+TGG 0.506286 8.1:+64404814 None:intergenic
AGCATGTGTCGAACCCTCCT+AGG 0.506559 8.1:+64401286 None:intergenic
AAGTCCAACATACATTGTTA+AGG 0.507953 8.1:-64404733 MS.gene016581:CDS
ACTAACATTCCTAAAACTTG+TGG 0.509218 8.1:+64404587 None:intergenic
ATTCCTTGTTCTGGTTGTGT+TGG 0.509240 8.1:-64404645 MS.gene016581:CDS
GAGGATGTTGTCTGGTGGTA+GGG 0.510247 8.1:+64401206 None:intergenic
TTAAGTCGCCTAACTTTAGT+GGG 0.511725 8.1:-64402544 MS.gene016581:CDS
TTAGTGGGTCGTTGAGACTT+GGG 0.514645 8.1:-64402529 MS.gene016581:CDS
CTATGTATGAGGATGTTGTC+TGG 0.515277 8.1:+64401198 None:intergenic
AATAGAGTGATGATCTTCTA+GGG 0.515833 8.1:+64402459 None:intergenic
TGGTTCTCCTCAGTGCAAGC+AGG 0.516456 8.1:-64404562 MS.gene016581:intron
TTAGCCTTAACAATGTATGT+TGG 0.520799 8.1:+64404729 None:intergenic
GGTCCTTAGCTTCTAGCTGT+AGG 0.521564 8.1:+64404835 None:intergenic
TTTGGGCCAAGAGCGACAAT+GGG 0.530197 8.1:+64402612 None:intergenic
TCGGCGAAACTCGTTTCTTA+CGG 0.530256 8.1:+64401338 None:intergenic
CACGCGCTTCCACCGCAAGT+GGG 0.533182 8.1:+64402797 None:intergenic
TGGGTCGTTGAGACTTGGGC+CGG 0.534117 8.1:-64402525 MS.gene016581:CDS
GGGAGATGATGAGGCAGAGA+AGG 0.536111 8.1:+64404982 None:intergenic
CTGGCGTTCAACCCAACTAC+CGG 0.536552 8.1:-64402377 MS.gene016581:CDS
TGATTTCCTAGCAACTAAGC+TGG 0.540455 8.1:+64404791 None:intergenic
AAATGTGACATCCAAGATGA+TGG 0.542321 8.1:-64404939 MS.gene016581:CDS
ATACTAGTAGTGATGCTGCT+TGG 0.543500 8.1:-64404668 MS.gene016581:CDS
ACCATCAAAACCAATGTCAT+GGG 0.556771 8.1:-64404868 MS.gene016581:CDS
TGGTTGCTATTAAAGTTGGC+CGG 0.557965 8.1:-64402427 MS.gene016581:CDS
ACACTTTCTTCCCATGACAT+TGG 0.559822 8.1:+64404858 None:intergenic
TGAGTGTTGAGCCATCATCT+TGG 0.560394 8.1:+64404928 None:intergenic
TTCTTCCATAGCAGCTAGTG+TGG 0.561075 8.1:-64402750 MS.gene016581:CDS
AAACTACCTGTGACAACCCT+AGG 0.568218 8.1:-64401303 MS.gene016581:CDS
TGTCCTACAGCTAGAAGCTA+AGG 0.572859 8.1:-64404838 MS.gene016581:CDS
GTTATCGGGTGCTCCTGCCA+TGG 0.574666 8.1:+64401149 None:intergenic
AACGAGTTTCGCCGACGAGT+TGG 0.576490 8.1:-64401330 MS.gene016581:CDS
AAGTTAGGCGACTTAAAGCT+AGG 0.577360 8.1:+64402551 None:intergenic
AACCCTCCTAGGGTTGTCAC+AGG 0.582519 8.1:+64401297 None:intergenic
TCCTGCCATGGCCAAACATG+TGG 0.582611 8.1:+64401161 None:intergenic
GCATGTGTCGAACCCTCCTA+GGG 0.582815 8.1:+64401287 None:intergenic
CTACCTGTGACAACCCTAGG+AGG 0.586431 8.1:-64401300 MS.gene016581:CDS
ACGCGCTTCCACCGCAAGTG+GGG 0.589037 8.1:+64402798 None:intergenic
ACACAGCCAAAAGTGTACCT+TGG 0.590538 8.1:+64402692 None:intergenic
CAACCAACACAACCAGAACA+AGG 0.590941 8.1:+64404642 None:intergenic
TTGAACGCCAGCGCAGCCGG+AGG 0.592132 8.1:+64402389 None:intergenic
TAGCACCACATGTTTGGCCA+TGG 0.594751 8.1:-64401166 MS.gene016581:CDS
AAAGCCAAAAGCAAAGTTTG+AGG 0.597951 8.1:+64404693 None:intergenic
GAAAATCGTCGATATCCCTC+CGG 0.600442 8.1:-64402405 MS.gene016581:CDS
AGGCAAACAGTAAGAGAATG+TGG 0.601674 8.1:+64402571 None:intergenic
GCCAAAAGCAAAGTTTGAGG+TGG 0.603568 8.1:+64404696 None:intergenic
ACACGCGCTTCCACCGCAAG+TGG 0.606518 8.1:+64402796 None:intergenic
TGAACGCCAGCGCAGCCGGA+GGG 0.607788 8.1:+64402390 None:intergenic
ATCCTCATACATAGCACCGC+CGG 0.613653 8.1:-64401189 MS.gene016581:CDS
ATGGTTTGAATGGAGAACAT+TGG 0.614704 8.1:+64404886 None:intergenic
TGGTAGGGTTACATTCATGC+CGG 0.624318 8.1:+64401221 None:intergenic
GTACCTTGGAATTGGGTCAG+TGG 0.625290 8.1:+64402706 None:intergenic
GAGAACAAGAATGTTATCGT+TGG 0.633329 8.1:+64401243 None:intergenic
GTACCCAACCCCACTTGCGG+TGG 0.634233 8.1:-64402806 MS.gene016581:CDS
CAGGTACCCAACCCCACTTG+CGG 0.634918 8.1:-64402809 MS.gene016581:CDS
CTTGGACTTGGGAGATGATG+AGG 0.637478 8.1:+64404973 None:intergenic
TACCGGCGGTGCTATGTATG+AGG 0.638539 8.1:+64401187 None:intergenic
CAGTTGTACCAATTGCATCA+GGG 0.640171 8.1:-64404770 MS.gene016581:CDS
TACCTGTGACAACCCTAGGA+GGG 0.643334 8.1:-64401299 MS.gene016581:CDS
TAGCCACTGACCCAATTCCA+AGG 0.643632 8.1:-64402709 MS.gene016581:CDS
AGCACCCGATAACGTGAACT+CGG 0.644320 8.1:-64401139 MS.gene016581:CDS
GCGAAACTCGTTTCTTACGG+CGG 0.651651 8.1:+64401341 None:intergenic
GGAAGTTCAGCACCACAACA+GGG 0.652373 8.1:-64402656 MS.gene016581:CDS
GGCCGGTGTATCAGCCCAAG+AGG 0.653863 8.1:-64402508 MS.gene016581:CDS
TCTAGGGTTTCTGAGAAGTG+GGG 0.654621 8.1:+64402475 None:intergenic
AAGCCCAATAGACCCTGTTG+TGG 0.656487 8.1:+64402644 None:intergenic
TGTATGAGGATGTTGTCTGG+TGG 0.659104 8.1:+64401201 None:intergenic
CAAACATGTGGTGCTACCGG+CGG 0.675349 8.1:+64401173 None:intergenic
TGGGCACATCAAATAGCACA+CGG 0.733939 8.1:+64401079 None:intergenic
TTACTAACCTGCTTGCACTG+AGG 0.771908 8.1:+64404555 None:intergenic
GCACATCAAATAGCACACGG+TGG 0.801608 8.1:+64401082 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATACTAATTTGTATTTA+AGG - chr8.1:64404579-64404598 MS.gene016581:CDS 10.0%
!!! TATATTGTTCAAATTTTTTA+TGG + chr8.1:64402459-64402478 None:intergenic 10.0%
!! AAAATGTCAATTTCAAAAAA+AGG + chr8.1:64401586-64401605 None:intergenic 15.0%
!! AACAATAGTAATTAGTTAAA+TGG - chr8.1:64404496-64404515 MS.gene016581:intron 15.0%
!! TAGATGTATAATTTCTTAAT+TGG + chr8.1:64402945-64402964 None:intergenic 15.0%
!! TGTTTCTTTAATGAAAAAAA+TGG + chr8.1:64404386-64404405 None:intergenic 15.0%
!! TTAGAAAATTTAAACCTAAT+TGG - chr8.1:64402386-64402405 MS.gene016581:CDS 15.0%
!!! ATTTTTTTCATTAAAGAAAC+AGG - chr8.1:64404385-64404404 MS.gene016581:intron 15.0%
!!! TGTCTAAAAATTTAAGATTT+TGG + chr8.1:64402489-64402508 None:intergenic 15.0%
!!! TTTTGAAATTGACATTTTTA+TGG - chr8.1:64401588-64401607 MS.gene016581:intron 15.0%
!!! TTTTTGTTAATATTTCGTTA+TGG + chr8.1:64402259-64402278 None:intergenic 15.0%
!! AATTACTATTGTTATATCCT+TGG + chr8.1:64404489-64404508 None:intergenic 20.0%
!! ACTAACATATCAAATAACTT+TGG - chr8.1:64403898-64403917 MS.gene016581:intron 20.0%
!! ATATTACAAAGTCATACATA+TGG + chr8.1:64404263-64404282 None:intergenic 20.0%
!! ATTATTACCTGAATCAAAAA+TGG + chr8.1:64403709-64403728 None:intergenic 20.0%
!! TACATATACACTTTATTAGA+AGG + chr8.1:64401738-64401757 None:intergenic 20.0%
!! TATATATACCTTCAAAACAT+GGG - chr8.1:64404097-64404116 MS.gene016581:intron 20.0%
!! TCTCAAACAAAAAAAAACTT+TGG - chr8.1:64403845-64403864 MS.gene016581:intron 20.0%
!! TTAAATGGTTTATACAATGT+CGG - chr8.1:64404511-64404530 MS.gene016581:intron 20.0%
!! TTATATATACCTTCAAAACA+TGG - chr8.1:64404096-64404115 MS.gene016581:intron 20.0%
!! TTTACCAAAATTATCCAATT+AGG + chr8.1:64402403-64402422 None:intergenic 20.0%
!!! AAATAGCTTTTGGTTTTTTT+GGG + chr8.1:64403151-64403170 None:intergenic 20.0%
!!! ATAGTTGAAAAGTTGATATA+TGG - chr8.1:64402281-64402300 MS.gene016581:intron 20.0%
!!! ATCATTGTTATTTTCAACTT+TGG - chr8.1:64403809-64403828 MS.gene016581:intron 20.0%
!!! ATTTGATAATGAACCAAATT+TGG - chr8.1:64402162-64402181 MS.gene016581:intron 20.0%
!!! CAAAAAGTACAAAACATAAA+CGG - chr8.1:64403191-64403210 MS.gene016581:intron 20.0%
!!! GAAAAGTAATAAATAGCTTT+TGG + chr8.1:64403161-64403180 None:intergenic 20.0%
!!! TAAACCTAATTGGATAATTT+TGG - chr8.1:64402396-64402415 MS.gene016581:CDS 20.0%
!!! TAAATAGCTTTTGGTTTTTT+TGG + chr8.1:64403152-64403171 None:intergenic 20.0%
!!! TTAATTTGTTTTCTTGCTAT+AGG + chr8.1:64402727-64402746 None:intergenic 20.0%
!!! TTGTTCAAATTTTTTATGGT+TGG + chr8.1:64402455-64402474 None:intergenic 20.0%
!!! TTTATAGTTTTGAAAAGTGT+CGG + chr8.1:64402750-64402769 None:intergenic 20.0%
!!! TTTATGTTTTGTACTTTTTG+TGG + chr8.1:64403191-64403210 None:intergenic 20.0%
!!! TTTGTTTTGTAAAAAAGTGT+CGG + chr8.1:64402837-64402856 None:intergenic 20.0%
! AAACACACATGCATTATTAT+TGG + chr8.1:64403969-64403988 None:intergenic 25.0%
! AAATACACAAGATAAAACCT+AGG + chr8.1:64404060-64404079 None:intergenic 25.0%
! AATACACAAGATAAAACCTA+GGG + chr8.1:64404059-64404078 None:intergenic 25.0%
! ATATATACCTTCAAAACATG+GGG - chr8.1:64404098-64404117 MS.gene016581:intron 25.0%
! ATCACTCTATTATGTTAACT+TGG - chr8.1:64403605-64403624 MS.gene016581:intron 25.0%
! CATTATACATCAACCAAATT+TGG + chr8.1:64402178-64402197 None:intergenic 25.0%
! CTACTCTTACATGAAATATA+AGG + chr8.1:64402347-64402366 None:intergenic 25.0%
! TAATGAAAAAAATGGATGTG+CGG + chr8.1:64404378-64404397 None:intergenic 25.0%
! TGTTAATATTTCGTTATGGA+GGG + chr8.1:64402255-64402274 None:intergenic 25.0%
! TTATTCTAACTATGTTACCA+AGG - chr8.1:64404469-64404488 MS.gene016581:intron 25.0%
! TTGTTAATATTTCGTTATGG+AGG + chr8.1:64402256-64402275 None:intergenic 25.0%
!! AAAACTTTGGACATGTATTT+TGG - chr8.1:64403858-64403877 MS.gene016581:intron 25.0%
!! AATTGACATTTTTATGGCAT+TGG - chr8.1:64401594-64401613 MS.gene016581:intron 25.0%
!! TATAAGCACCCTTTTATTAT+AGG - chr8.1:64402628-64402647 MS.gene016581:CDS 25.0%
!! TCAAACATGTGATATTTTTC+AGG - chr8.1:64403224-64403243 MS.gene016581:intron 25.0%
!! TTTTCTTGTTGAATATTACC+AGG - chr8.1:64404653-64404672 MS.gene016581:CDS 25.0%
!!! AATAGCTTTTGGTTTTTTTG+GGG + chr8.1:64403150-64403169 None:intergenic 25.0%
!!! ATAAAAGGGTGCTTATATAT+AGG + chr8.1:64402625-64402644 None:intergenic 25.0%
!!! ATGAATTAACTCTTTTTTGG+TGG + chr8.1:64402033-64402052 None:intergenic 25.0%
!!! GATAATTTTGGTAAAGACTA+GGG - chr8.1:64402408-64402427 MS.gene016581:CDS 25.0%
!!! GATTATTAGTTTCTTTTCTC+TGG - chr8.1:64401889-64401908 MS.gene016581:intron 25.0%
!!! GTGATGAATTAACTCTTTTT+TGG + chr8.1:64402036-64402055 None:intergenic 25.0%
!!! TAAAAGGGTGCTTATATATA+GGG + chr8.1:64402624-64402643 None:intergenic 25.0%
AACAATACCAATTCTTGAGT+TGG + chr8.1:64404996-64405015 None:intergenic 30.0%
AAGTCCAACATACATTGTTA+AGG - chr8.1:64401319-64401338 MS.gene016581:CDS 30.0%
AATAGAGTGATGATCTTCTA+GGG + chr8.1:64403596-64403615 None:intergenic 30.0%
ACAATACCAATTCTTGAGTT+GGG + chr8.1:64404995-64405014 None:intergenic 30.0%
ACCCTTTGCCTATAATAAAA+GGG + chr8.1:64402639-64402658 None:intergenic 30.0%
ACGTAATCTTGATCTCAAAA+TGG + chr8.1:64404330-64404349 None:intergenic 30.0%
ACTAACATTCCTAAAACTTG+TGG + chr8.1:64401468-64401487 None:intergenic 30.0%
ATTCTCACGAAAAAACTAAG+TGG - chr8.1:64404151-64404170 MS.gene016581:intron 30.0%
TAATAGAGTGATGATCTTCT+AGG + chr8.1:64403597-64403616 None:intergenic 30.0%
TAATCTTGATCTCAAAATGG+TGG + chr8.1:64404327-64404346 None:intergenic 30.0%
TACATTGTTAAGGCTAAGTT+TGG - chr8.1:64401329-64401348 MS.gene016581:CDS 30.0%
TATTATGCTTGACCAACAAA+GGG - chr8.1:64403997-64404016 MS.gene016581:intron 30.0%
TCAATACTTACCTTTCCTAT+AGG - chr8.1:64403105-64403124 MS.gene016581:intron 30.0%
TGCAGATAGTATTATGTCTT+TGG + chr8.1:64402058-64402077 None:intergenic 30.0%
TTATCGTTGGTACAACTATT+GGG + chr8.1:64404799-64404818 None:intergenic 30.0%
TTCACTTAAGCTTATGCTAT+TGG - chr8.1:64401852-64401871 MS.gene016581:intron 30.0%
TTCTCACGAAAAAACTAAGT+GGG - chr8.1:64404152-64404171 MS.gene016581:intron 30.0%
! AACTTGGTTGCTATTAAAGT+TGG - chr8.1:64403621-64403640 MS.gene016581:intron 30.0%
! AATTTTGGATCTTGCTAACA+AGG - chr8.1:64402902-64402921 MS.gene016581:intron 30.0%
! ACCCTTTTATTATAGGCAAA+GGG - chr8.1:64402635-64402654 MS.gene016581:CDS 30.0%
!! AGGAAAGGTGTTAGAAAAAT+TGG + chr8.1:64401053-64401072 None:intergenic 30.0%
!! GGAAAGGTGTTAGAAAAATT+GGG + chr8.1:64401052-64401071 None:intergenic 30.0%
!! GGATAATTTTGGTAAAGACT+AGG - chr8.1:64402407-64402426 MS.gene016581:CDS 30.0%
!! TTAGCCTTAACAATGTATGT+TGG + chr8.1:64401326-64401345 None:intergenic 30.0%
!! TTTTATGGCATTGGAATGTT+TGG - chr8.1:64401603-64401622 MS.gene016581:intron 30.0%
!!! AGTTTTAGGAATGTTAGTTG+TGG - chr8.1:64401470-64401489 MS.gene016581:intron 30.0%
!!! ATCTTTTTTGCCTATAGGAA+AGG + chr8.1:64403118-64403137 None:intergenic 30.0%
!!! ATTGGTTTTGATGGTTTGAA+TGG + chr8.1:64401179-64401198 None:intergenic 30.0%
!!! TAAGCTGTTTTTGAAAGTGT+CGG + chr8.1:64402783-64402802 None:intergenic 30.0%
AAAGCCAAAAGCAAAGTTTG+AGG + chr8.1:64401362-64401381 None:intergenic 35.0%
AAATGTGACATCCAAGATGA+TGG - chr8.1:64401113-64401132 MS.gene016581:CDS 35.0%
AACCATCAAAACCAATGTCA+TGG - chr8.1:64401183-64401202 MS.gene016581:CDS 35.0%
ACCATCAAAACCAATGTCAT+GGG - chr8.1:64401184-64401203 MS.gene016581:CDS 35.0%
ACGATAACATTCTTGTTCTC+CGG - chr8.1:64404812-64404831 MS.gene016581:CDS 35.0%
ATGGTTTGAATGGAGAACAT+TGG + chr8.1:64401169-64401188 None:intergenic 35.0%
ATGTAGTTACGTGTGATGAT+TGG + chr8.1:64402002-64402021 None:intergenic 35.0%
CACCCTTTGCCTATAATAAA+AGG + chr8.1:64402640-64402659 None:intergenic 35.0%
CGTGTGTGATGACAAAATTA+AGG - chr8.1:64404195-64404214 MS.gene016581:intron 35.0%
CTAAAACTTGTGGACTTAAC+AGG + chr8.1:64401458-64401477 None:intergenic 35.0%
CTATTATGCTTGACCAACAA+AGG - chr8.1:64403996-64404015 MS.gene016581:intron 35.0%
GAGAACAAGAATGTTATCGT+TGG + chr8.1:64404812-64404831 None:intergenic 35.0%
GTTATCGTTGGTACAACTAT+TGG + chr8.1:64404800-64404819 None:intergenic 35.0%
TCATACGATTAATCTTCCCT+AGG - chr8.1:64404040-64404059 MS.gene016581:intron 35.0%
TGTGCATTCAACTTCACTTA+TGG - chr8.1:64403276-64403295 MS.gene016581:intron 35.0%
TTAAGTCGCCTAACTTTAGT+GGG - chr8.1:64403508-64403527 MS.gene016581:intron 35.0%
TTGATCTCAAAATGGTGGTT+GGG + chr8.1:64404322-64404341 None:intergenic 35.0%
TTTAAGTCGCCTAACTTTAG+TGG - chr8.1:64403507-64403526 MS.gene016581:intron 35.0%
! ACAAGTATTGCATTCTAGCT+TGG + chr8.1:64401241-64401260 None:intergenic 35.0%
! AGGGTGCTTATATATAAGGA+CGG - chr8.1:64402654-64402673 MS.gene016581:CDS 35.0%
! ATCTTGGATGTCACATTTTG+GGG + chr8.1:64401111-64401130 None:intergenic 35.0%
! ATGTGGACTTGTAGAGATTT+TGG + chr8.1:64403467-64403486 None:intergenic 35.0%
! CAAAAGTGTACCTTGGAATT+GGG + chr8.1:64403356-64403375 None:intergenic 35.0%
! CACCCTTTTATTATAGGCAA+AGG - chr8.1:64402634-64402653 MS.gene016581:CDS 35.0%
! CATCTTGGATGTCACATTTT+GGG + chr8.1:64401112-64401131 None:intergenic 35.0%
! CTGTTAAGTCCACAAGTTTT+AGG - chr8.1:64401456-64401475 MS.gene016581:intron 35.0%
! TCATCTTGGATGTCACATTT+TGG + chr8.1:64401113-64401132 None:intergenic 35.0%
!! GCAAAGGGTGCTTATATATA+AGG - chr8.1:64402650-64402669 MS.gene016581:CDS 35.0%
!! TGCTTTTTGTACAAGACATG+TGG + chr8.1:64404299-64404318 None:intergenic 35.0%
!!! ACTTGTAGAGATTTTGGCTT+TGG + chr8.1:64403461-64403480 None:intergenic 35.0%
!!! CTTGTAGAGATTTTGGCTTT+GGG + chr8.1:64403460-64403479 None:intergenic 35.0%
!!! TCAAACTTTGCTTTTGGCTT+TGG - chr8.1:64401361-64401380 MS.gene016581:CDS 35.0%
!!! TCGTGATCTTTTTTGCCTAT+AGG + chr8.1:64403123-64403142 None:intergenic 35.0%
AAGTTAGGCGACTTAAAGCT+AGG + chr8.1:64403504-64403523 None:intergenic 40.0%
ACACTTTCTTCCCATGACAT+TGG + chr8.1:64401197-64401216 None:intergenic 40.0%
AGACTAGGGTAGATTCACAT+TGG - chr8.1:64402422-64402441 MS.gene016581:CDS 40.0%
AGGCAAACAGTAAGAGAATG+TGG + chr8.1:64403484-64403503 None:intergenic 40.0%
ATACTAGTAGTGATGCTGCT+TGG - chr8.1:64401384-64401403 MS.gene016581:intron 40.0%
ATCAAAAATGGTTCCAGCAC+CGG + chr8.1:64403697-64403716 None:intergenic 40.0%
ATTTGTCTCCCTGATGCAAT+TGG + chr8.1:64401293-64401312 None:intergenic 40.0%
CAGTTGTACCAATTGCATCA+GGG - chr8.1:64401282-64401301 MS.gene016581:CDS 40.0%
CTATGTATGAGGATGTTGTC+TGG + chr8.1:64404857-64404876 None:intergenic 40.0%
CTTGATCTCAAAATGGTGGT+TGG + chr8.1:64404323-64404342 None:intergenic 40.0%
GACTAGGGTAGATTCACATT+GGG - chr8.1:64402423-64402442 MS.gene016581:CDS 40.0%
GGCTGTGTTAACAAAACAAC+AGG - chr8.1:64403375-64403394 MS.gene016581:intron 40.0%
TAGTTACGTGTGATGATTGG+TGG + chr8.1:64401999-64402018 None:intergenic 40.0%
TCAAAATCCTACACGTGACT+TGG - chr8.1:64404237-64404256 MS.gene016581:intron 40.0%
TCAGTTGTACCAATTGCATC+AGG - chr8.1:64401281-64401300 MS.gene016581:CDS 40.0%
TGATTTCCTAGCAACTAAGC+TGG + chr8.1:64401264-64401283 None:intergenic 40.0%
TGCTTATATATAGGGACGGA+GGG + chr8.1:64402616-64402635 None:intergenic 40.0%
! AGGGTGCTTATATATAGGGA+CGG + chr8.1:64402620-64402639 None:intergenic 40.0%
! ATTCCTTGTTCTGGTTGTGT+TGG - chr8.1:64401407-64401426 MS.gene016581:intron 40.0%
! CCAAAAGTGTACCTTGGAAT+TGG + chr8.1:64403357-64403376 None:intergenic 40.0%
! CCAATTCCAAGGTACACTTT+TGG - chr8.1:64403354-64403373 MS.gene016581:intron 40.0%
! CTCAACACTACTTGTTAGCT+TGG - chr8.1:64401528-64401547 MS.gene016581:intron 40.0%
! GAGAACATTGGCTGAAGATA+TGG + chr8.1:64401157-64401176 None:intergenic 40.0%
! TCCACCTCAAACTTTGCTTT+TGG - chr8.1:64401355-64401374 MS.gene016581:CDS 40.0%
! TGGTTGCTATTAAAGTTGGC+CGG - chr8.1:64403625-64403644 MS.gene016581:intron 40.0%
!! GCTGGAACCATTTTTGATTC+AGG - chr8.1:64403699-64403718 MS.gene016581:intron 40.0%
!! GGAAAGGTAAGTATTGACGA+TGG + chr8.1:64403102-64403121 None:intergenic 40.0%
!! TTCTAGGGTTTCTGAGAAGT+GGG + chr8.1:64403581-64403600 None:intergenic 40.0%
!!! GTGGTTTTGTTGTTGCATGT+TGG + chr8.1:64404954-64404973 None:intergenic 40.0%
!!! TCCCATGACATTGGTTTTGA+TGG + chr8.1:64401188-64401207 None:intergenic 40.0%
AAACTACCTGTGACAACCCT+AGG - chr8.1:64404749-64404768 MS.gene016581:CDS 45.0%
AACACCGAGTTCACGTTATC+GGG + chr8.1:64404920-64404939 None:intergenic 45.0%
ACTTGTCCAGCTTAGTTGCT+AGG - chr8.1:64401255-64401274 MS.gene016581:CDS 45.0%
AGTCGAGTGAATACAGTACC+TGG + chr8.1:64404674-64404693 None:intergenic 45.0%
ATGATGAGGCAGAGAAGGAA+AGG + chr8.1:64401068-64401087 None:intergenic 45.0%
CAACACCGAGTTCACGTTAT+CGG + chr8.1:64404921-64404940 None:intergenic 45.0%
CAACCAACACAACCAGAACA+AGG + chr8.1:64401413-64401432 None:intergenic 45.0%
CACCTACTAGTTCCCTTTGT+TGG + chr8.1:64404012-64404031 None:intergenic 45.0%
CTCAACGACCCACTAAAGTT+AGG + chr8.1:64403519-64403538 None:intergenic 45.0%
GAAAATCGTCGATATCCCTC+CGG - chr8.1:64403647-64403666 MS.gene016581:intron 45.0%
GACCAACAAAGGGAACTAGT+AGG - chr8.1:64404007-64404026 MS.gene016581:intron 45.0%
GATGTGCCCAACTCAAGAAT+TGG - chr8.1:64404986-64405005 MS.gene016581:CDS 45.0%
GCCAAAAGCAAAGTTTGAGG+TGG + chr8.1:64401359-64401378 None:intergenic 45.0%
GGGGTATACTTGATCCTCTT+GGG + chr8.1:64403561-64403580 None:intergenic 45.0%
GTGCTTATATATAGGGACGG+AGG + chr8.1:64402617-64402636 None:intergenic 45.0%
GTGGACTTAACAGGAGCAAA+AGG + chr8.1:64401449-64401468 None:intergenic 45.0%
TCGGCGAAACTCGTTTCTTA+CGG + chr8.1:64404717-64404736 None:intergenic 45.0%
TGGGCACATCAAATAGCACA+CGG + chr8.1:64404976-64404995 None:intergenic 45.0%
TGGGGTATACTTGATCCTCT+TGG + chr8.1:64403562-64403581 None:intergenic 45.0%
TGGTAGGGTTACATTCATGC+CGG + chr8.1:64404834-64404853 None:intergenic 45.0%
TGTATGAGGATGTTGTCTGG+TGG + chr8.1:64404854-64404873 None:intergenic 45.0%
TGTCCTACAGCTAGAAGCTA+AGG - chr8.1:64401214-64401233 MS.gene016581:CDS 45.0%
TTACTAACCTGCTTGCACTG+AGG + chr8.1:64401500-64401519 None:intergenic 45.0%
TTCTTCCATAGCAGCTAGTG+TGG - chr8.1:64403302-64403321 MS.gene016581:intron 45.0%
TTTCTTACGGCGGTGTATAC+AGG + chr8.1:64404704-64404723 None:intergenic 45.0%
! ACACAGCCAAAAGTGTACCT+TGG + chr8.1:64403363-64403382 None:intergenic 45.0%
! AGGGTTGTCACAGGTAGTTT+TGG + chr8.1:64404749-64404768 None:intergenic 45.0%
! GAGGGATATCGACGATTTTC+CGG + chr8.1:64403647-64403666 None:intergenic 45.0%
! TCTAGGGTTTCTGAGAAGTG+GGG + chr8.1:64403580-64403599 None:intergenic 45.0%
! TTAGTGGGTCGTTGAGACTT+GGG - chr8.1:64403523-64403542 MS.gene016581:intron 45.0%
! TTTAGTGGGTCGTTGAGACT+TGG - chr8.1:64403522-64403541 MS.gene016581:intron 45.0%
!! CTTCTAGGGTTTCTGAGAAG+TGG + chr8.1:64403582-64403601 None:intergenic 45.0%
!! GATTGTGCCCCATGTTTTGA+AGG + chr8.1:64404108-64404127 None:intergenic 45.0%
!! TGAGTGTTGAGCCATCATCT+TGG + chr8.1:64401127-64401146 None:intergenic 45.0%
!!! CACATTTTGGGGTTTGAGCT+TGG + chr8.1:64401100-64401119 None:intergenic 45.0%
!!! GTAGTTTTGGACCAACTCGT+CGG + chr8.1:64404736-64404755 None:intergenic 45.0%
!! TATATAAATATAAAATAGAA+AGG + chr8.1:64404083-64404102 None:intergenic 5.0%
!!! AATTAAACATTTATAAATTT+TGG - chr8.1:64402887-64402906 MS.gene016581:intron 5.0%
AAGCCCAATAGACCCTGTTG+TGG + chr8.1:64403411-64403430 None:intergenic 50.0%
ACAACAGGGTCTATTGGGCT+TGG - chr8.1:64403410-64403429 MS.gene016581:intron 50.0%
AGCACCACAACAGGGTCTAT+TGG - chr8.1:64403404-64403423 MS.gene016581:intron 50.0%
AGCACCCGATAACGTGAACT+CGG - chr8.1:64404913-64404932 MS.gene016581:CDS 50.0%
AGGAAGTTCAGCACCACAAC+AGG - chr8.1:64403395-64403414 MS.gene016581:intron 50.0%
ATCCTCATACATAGCACCGC+CGG - chr8.1:64404863-64404882 MS.gene016581:CDS 50.0%
ATCCTCTTGGGCTGATACAC+CGG + chr8.1:64403549-64403568 None:intergenic 50.0%
CAACAGGGTCTATTGGGCTT+GGG - chr8.1:64403411-64403430 MS.gene016581:intron 50.0%
CATATGGCCAAGTCACGTGT+AGG + chr8.1:64404247-64404266 None:intergenic 50.0%
CTTGGACTTGGGAGATGATG+AGG + chr8.1:64401082-64401101 None:intergenic 50.0%
GAGGATGTTGTCTGGTGGTA+GGG + chr8.1:64404849-64404868 None:intergenic 50.0%
GCACATCAAATAGCACACGG+TGG + chr8.1:64404973-64404992 None:intergenic 50.0%
GCACCACAACAGGGTCTATT+GGG - chr8.1:64403405-64403424 MS.gene016581:intron 50.0%
GCGAAACTCGTTTCTTACGG+CGG + chr8.1:64404714-64404733 None:intergenic 50.0%
GCTGCTTGGATTCCTTGTTC+TGG - chr8.1:64401398-64401417 MS.gene016581:intron 50.0%
GGAAGTTCAGCACCACAACA+GGG - chr8.1:64403396-64403415 MS.gene016581:intron 50.0%
GTACCTTGGAATTGGGTCAG+TGG + chr8.1:64403349-64403368 None:intergenic 50.0%
TACCTGTGACAACCCTAGGA+GGG - chr8.1:64404753-64404772 MS.gene016581:CDS 50.0%
TAGCACCACATGTTTGGCCA+TGG - chr8.1:64404886-64404905 MS.gene016581:CDS 50.0%
TAGCCACTGACCCAATTCCA+AGG - chr8.1:64403343-64403362 MS.gene016581:intron 50.0%
TGAGGATGTTGTCTGGTGGT+AGG + chr8.1:64404850-64404869 None:intergenic 50.0%
TGGAATTGGGTCAGTGGCTA+GGG + chr8.1:64403343-64403362 None:intergenic 50.0%
TGGCTAGGGTAAGTGTGTCT+TGG + chr8.1:64403329-64403348 None:intergenic 50.0%
TGGGGTTTGAGCTTGGACTT+GGG + chr8.1:64401093-64401112 None:intergenic 50.0%
TTGGAATTGGGTCAGTGGCT+AGG + chr8.1:64403344-64403363 None:intergenic 50.0%
TTGGACCACACTAGCTGCTA+TGG + chr8.1:64403310-64403329 None:intergenic 50.0%
TTGGGGTTTGAGCTTGGACT+TGG + chr8.1:64401094-64401113 None:intergenic 50.0%
TTTGGGCCAAGAGCGACAAT+GGG + chr8.1:64403443-64403462 None:intergenic 50.0%
! GGTCCTTAGCTTCTAGCTGT+AGG + chr8.1:64401220-64401239 None:intergenic 50.0%
AACCCTCCTAGGGTTGTCAC+AGG + chr8.1:64404758-64404777 None:intergenic 55.0%
AACGAGTTTCGCCGACGAGT+TGG - chr8.1:64404722-64404741 MS.gene016581:CDS 55.0%
ACCACATGTTTGGCCATGGC+AGG - chr8.1:64404890-64404909 MS.gene016581:CDS 55.0%
AGCATGTGTCGAACCCTCCT+AGG + chr8.1:64404769-64404788 None:intergenic 55.0%
CTACCTGTGACAACCCTAGG+AGG - chr8.1:64404752-64404771 MS.gene016581:CDS 55.0%
CTGGCGTTCAACCCAACTAC+CGG - chr8.1:64403675-64403694 MS.gene016581:intron 55.0%
CTTTGGGCCAAGAGCGACAA+TGG + chr8.1:64403444-64403463 None:intergenic 55.0%
GCATGTGTCGAACCCTCCTA+GGG + chr8.1:64404768-64404787 None:intergenic 55.0%
GGGAGATGATGAGGCAGAGA+AGG + chr8.1:64401073-64401092 None:intergenic 55.0%
TCCTGCCATGGCCAAACATG+TGG + chr8.1:64404894-64404913 None:intergenic 55.0%
TGGTTCTCCTCAGTGCAAGC+AGG - chr8.1:64401490-64401509 MS.gene016581:intron 55.0%
TTCAACCCAACTACCGGTGC+TGG - chr8.1:64403681-64403700 MS.gene016581:intron 55.0%
!! ATGGTTCCAGCACCGGTAGT+TGG + chr8.1:64403690-64403709 None:intergenic 55.0%
!! CAAACATGTGGTGCTACCGG+CGG + chr8.1:64404882-64404901 None:intergenic 55.0%
!! GGCCAAACATGTGGTGCTAC+CGG + chr8.1:64404885-64404904 None:intergenic 55.0%
!! TACCGGCGGTGCTATGTATG+AGG + chr8.1:64404868-64404887 None:intergenic 55.0%
!! TGGTTCCAGCACCGGTAGTT+GGG + chr8.1:64403689-64403708 None:intergenic 55.0%
CAGGTACCCAACCCCACTTG+CGG - chr8.1:64403243-64403262 MS.gene016581:intron 60.0%
CCAAGAGCGACAATGGGCCT+CGG + chr8.1:64403437-64403456 None:intergenic 60.0%
CGCCGGTAGCACCACATGTT+TGG - chr8.1:64404880-64404899 MS.gene016581:CDS 60.0%
CTTCCACCGCAAGTGGGGTT+GGG + chr8.1:64403252-64403271 None:intergenic 60.0%
GTTATCGGGTGCTCCTGCCA+TGG + chr8.1:64404906-64404925 None:intergenic 60.0%
TGGGTCGTTGAGACTTGGGC+CGG - chr8.1:64403527-64403546 MS.gene016581:intron 60.0%
ACACGCGCTTCCACCGCAAG+TGG + chr8.1:64403259-64403278 None:intergenic 65.0%
ACGCGCTTCCACCGCAAGTG+GGG + chr8.1:64403257-64403276 None:intergenic 65.0%
CACGCGCTTCCACCGCAAGT+GGG + chr8.1:64403258-64403277 None:intergenic 65.0%
CCGAGGCCCATTGTCGCTCT+TGG - chr8.1:64403434-64403453 MS.gene016581:intron 65.0%
GCTTCCACCGCAAGTGGGGT+TGG + chr8.1:64403253-64403272 None:intergenic 65.0%
GGCCGGTGTATCAGCCCAAG+AGG - chr8.1:64403544-64403563 MS.gene016581:intron 65.0%
GGTCTATTGGGCTTGGGCCG+AGG - chr8.1:64403417-64403436 MS.gene016581:intron 65.0%
GTACCCAACCCCACTTGCGG+TGG - chr8.1:64403246-64403265 MS.gene016581:intron 65.0%
CGATATCCCTCCGGCTGCGC+TGG - chr8.1:64403656-64403675 MS.gene016581:intron 70.0%
GGGTTGAACGCCAGCGCAGC+CGG + chr8.1:64403669-64403688 None:intergenic 70.0%
TGAACGCCAGCGCAGCCGGA+GGG + chr8.1:64403665-64403684 None:intergenic 70.0%
TTGAACGCCAGCGCAGCCGG+AGG + chr8.1:64403666-64403685 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 64401041 64405033 64401041 ID=MS.gene016581
chr8.1 mRNA 64401041 64405033 64401041 ID=MS.gene016581.t1;Parent=MS.gene016581
chr8.1 exon 64404563 64405033 64404563 ID=MS.gene016581.t1.exon1;Parent=MS.gene016581.t1
chr8.1 CDS 64404563 64405033 64404563 ID=cds.MS.gene016581.t1;Parent=MS.gene016581.t1
chr8.1 exon 64402354 64402828 64402354 ID=MS.gene016581.t1.exon2;Parent=MS.gene016581.t1
chr8.1 CDS 64402354 64402828 64402354 ID=cds.MS.gene016581.t1;Parent=MS.gene016581.t1
chr8.1 exon 64401041 64401399 64401041 ID=MS.gene016581.t1.exon3;Parent=MS.gene016581.t1
chr8.1 CDS 64401041 64401399 64401041 ID=cds.MS.gene016581.t1;Parent=MS.gene016581.t1
Gene Sequence

>MS.gene016581

ATGAAAATCCCAATTTTTCTAACACCTTTCCTTCTCTGCCTCATCATCTCCCAAGTCCAAGCTCAAACCCCAAAATGTGACATCCAAGATGATGGCTCAACACTCAAAGTGTTCCATATCTTCAGCCAATGTTCTCCATTCAAACCATCAAAACCAATGTCATGGGAAGAAAGTGTCCTACAGCTAGAAGCTAAGGACCAAGCTAGAATGCAATACTTGTCCAGCTTAGTTGCTAGGAAATCAGTTGTACCAATTGCATCAGGGAGACAAATCATACAAAGTCCAACATACATTGTTAAGGCTAAGTTTGGTACTCCACCTCAAACTTTGCTTTTGGCTTTGGATACTAGTAGTGATGCTGCTTGGATTCCTTGTTCTGGTTGTGTTGGTTGCTCAACTTCAAAACCTTTTGCTCCTGTTAAGTCCACAAGTTTTAGGAATGTTAGTTGTGGTTCTCCTCAGTGCAAGCAGGTTAGTAATTAACATACTCAACACTACTTGTTAGCTTGGATTTTTTTCTTGCATCTTTTTTAGTTCTTTTACCTTTTTTTGAAATTGACATTTTTATGGCATTGGAATGTTTGGATCTCATTGTTTTGATTATATAATATGATATTGATTGTGACTAGTATATATGTGTGTGTCTATATATAATATGTGTGTCTCTCATTATTTAGAAAAGTATTTAATTTGTCCTTCTAATAAAGTGTATATGTACTAGTAATATCTAGTGATTTATTTTAACTTAATTTGATCTGAAGTGTATAATGTTGACAATGTTGAAACTTATTAAAATTTAAGAGTGAAATTATTCACTTAAGCTTATGCTATTGGTAAAATTACTTTAAGATTATTAGTTTCTTTTCTCTGGTTTCAATATTATTTGTTTCTTTTAACTCAAAAAATTATTTGTTTCTCTATCTCATCACACGTAACTACATAATAGTATATCTCACCACCAATCATCACACGTAACTACATAATAGTAACCACCAAAAAAGAGTTAATTCATCACCAAAGACATAATACTATCTGCAAGAGCTCTTGAACAATTTTTTATTTTGTTTCATAAGAAACTCTTAATATGATATATACGTGTGTGTGTGTCAAAATCAACATAAATTTGATAATGAACCAAATTTGGTTGATGTATAATGTGTTGACAGTTGATGAATCAAAATCAAAACAATATACATTTGATAAAATACATACCCTCCATAACGAAATATTAACAAAAATGATAGTTGAAAAGTTGATATATGGATTAAATTTTAGATACGTCAACATTTCAACTCTTGTTTTTCCTTATATTTCATGTAAGAGTAGTATAATATAAAATTCTTTGTTAGAAAATTTAAACCTAATTGGATAATTTTGGTAAAGACTAGGGTAGATTCACATTGGGTTAAAACCAACCATAAAAAATTTGAACAATATATCTTCATCCAAAATCTTAAATTTTTAGACATAGAATCATATTGTTTATACGTTATTCAATATCTATTTATCTTATTTAATGTGTTACTTCTAACTCAACAGATCAAACTATCTATGCATGCACTCAAATATACTCCCTCCGTCCCTATATATAAGCACCCTTTTATTATAGGCAAAGGGTGCTTATATATAAGGACGGAGAGAGTATAATCTATCAGACACTTATAAAAACAGCAGATGCTTACACCTATAGCAAGAAAACAAATTAACCGACACTTTTCAAAACTATAAAAACAAATTAACCGACACTTTCAAAAACAGCTTATTCTTGTATCTATAGCATGAAAACAAATTTACCGACACTTTTTTACAAAACAAAATAACTAACATTCTTCATTTAAATATAATAAATTAAACATTTATAAATTTTGGATCTTGCTAACAAGGTTACTTTTTAATAATTACCAATTAAGAAATTATACATCTAAAAAGTCTAAAACTTTAATCTACGATCAATTAAATACACAAATTCTTATTAAAATTTATTATCTAAATTCGTTAAAGAGTTCTCATAAATTTTTCACTATATTACGTAGTATATTCGAATCATAAAACTTTATTCCATCGTCAATACTTACCTTTCCTATAGGCAAAAAAGATCACGAGATTCCCCAAAAAAACCAAAAGCTATTTATTACTTTTCTATTTTTCCACAAAAAGTACAAAACATAAACGGTTCTCAAATCTCAAACATGTGATATTTTTCAGGTACCCAACCCCACTTGCGGTGGAAGCGCGTGTGCATTCAACTTCACTTATGGTAGTTCTTCCATAGCAGCTAGTGTGGTCCAAGACACACTTACCCTAGCCACTGACCCAATTCCAAGGTACACTTTTGGCTGTGTTAACAAAACAACAGGAAGTTCAGCACCACAACAGGGTCTATTGGGCTTGGGCCGAGGCCCATTGTCGCTCTTGGCCCAAAGCCAAAATCTCTACAAGTCCACATTCTCTTACTGTTTGCCTAGCTTTAAGTCGCCTAACTTTAGTGGGTCGTTGAGACTTGGGCCGGTGTATCAGCCCAAGAGGATCAAGTATACCCCACTTCTCAGAAACCCTAGAAGATCATCACTCTATTATGTTAACTTGGTTGCTATTAAAGTTGGCCGGAAAATCGTCGATATCCCTCCGGCTGCGCTGGCGTTCAACCCAACTACCGGTGCTGGAACCATTTTTGATTCAGGTAATAATGTCTTCATTAACATTTGACATATTCTAATTCTTAACAAAATGAATTCTAGTATAGTTTTACAAATCATAAGTATACATTTATCATTGTTATTTTCAACTTTGGACATGCACTCTTTTCTCAAACAAAAAAAAACTTTGGACATGTATTTTGGATAATGAAATTTCAGATACTAACATATCAAATAACTTTGGACATGTATTGTAAATAAATTTTTTACTACTAATAAATTTAAACTACCAATAATAATGCATGTGTGTTTAAGTTTACTATTATGCTTGACCAACAAAGGGAACTAGTAGGTGTTATGAGATCATACGATTAATCTTCCCTAGGTTTTATCTTGTGTATTTCCTTTCTATTTTATATTTATATATACCTTCAAAACATGGGGCACAATCTTGTGTAGTCATGTTATGTTCACATTCTCACGAAAAAACTAAGTGGGTTTCATGTTTTAACTTTTAACGTGTGTGATGACAAAATTAAGGTCAAGTTGAGACAAATTTGTCAAAATCCTACACGTGACTTGGCCATATGTATGACTTTGTAATATTATGTATCATGCTCCACATGTCTTGTACAAAAAGCACCCAACCACCATTTTGAGATCAAGATTACGTACAAGTACAATTATAATTATAATCACCGCACATCCATTTTTTTCATTAAAGAAACAGGAGAATGATGATTTTGAAAATAAATTGACAATATTGAAGCAAACAACAATAACACTAAGACATTATTCTAACTATGTTACCAAGGATATAACAATAGTAATTAGTTAAATGGTTTATACAATGTCGGTGTAAAAATTCATTTTATACATAAGCATACTTTGTAATCTTTCAAATAATACTAATTTGTATTTAAGGCGCATATTTTATTTTCTCATGTCAATTTCACTAAACATTTTCTAAATCTGATTTTCTTGTTGAATATTACCAGGTACTGTATTCACTCGACTAGCTGAACCTGTATACACCGCCGTAAGAAACGAGTTTCGCCGACGAGTTGGTCCAAAACTACCTGTGACAACCCTAGGAGGGTTCGACACATGCTACAATGTCCCAATAGTTGTACCAACGATAACATTCTTGTTCTCCGGCATGAATGTAACCCTACCACCAGACAACATCCTCATACATAGCACCGCCGGTAGCACCACATGTTTGGCCATGGCAGGAGCACCCGATAACGTGAACTCGGTGTTGAACGTGATAGCCAACATGCAACAACAAAACCACCGTGTGCTATTTGATGTGCCCAACTCAAGAATTGGTATTGTTCGTGAGCTTTGCACCTAA
Protein sequence

>MS.gene016581.t1

MKIPIFLTPFLLCLIISQVQAQTPKCDIQDDGSTLKVFHIFSQCSPFKPSKPMSWEESVLQLEAKDQARMQYLSSLVARKSVVPIASGRQIIQSPTYIVKAKFGTPPQTLLLALDTSSDAAWIPCSGCVGCSTSKPFAPVKSTSFRNVSCGSPQCKQVPNPTCGGSACAFNFTYGSSSIAASVVQDTLTLATDPIPRYTFGCVNKTTGSSAPQQGLLGLGRGPLSLLAQSQNLYKSTFSYCLPSFKSPNFSGSLRLGPVYQPKRIKYTPLLRNPRRSSLYYVNLVAIKVGRKIVDIPPAALAFNPTTGAGTIFDSGTVFTRLAEPVYTAVRNEFRRRVGPKLPVTTLGGFDTCYNVPIVVPTITFLFSGMNVTLPPDNILIHSTAGSTTCLAMAGAPDNVNSVLNVIANMQQQNHRVLFDVPNSRIGIVRELCT