AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene020419

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020419.t1 MTR_3g100500 93.812 404 25 0 1 404 17 420 0.0 773
MS.gene020419.t1 MTR_8g039540 52.346 405 181 7 1 400 16 413 7.17e-125 369
MS.gene020419.t1 MTR_1g037630 52.152 395 177 6 10 400 22 408 1.42e-124 368
MS.gene020419.t1 MTR_3g014290 53.134 367 160 6 38 400 38 396 3.08e-117 348
MS.gene020419.t1 MTR_1g039270 31.367 373 229 12 35 385 71 438 1.18e-39 148
MS.gene020419.t1 MTR_1g115210 34.259 324 200 8 71 385 136 455 3.69e-39 147
MS.gene020419.t1 MTR_7g117430 33.714 350 212 8 49 381 103 449 2.49e-38 144
MS.gene020419.t1 MTR_3g097150 32.153 367 211 14 42 383 61 414 6.63e-38 143
MS.gene020419.t1 MTR_4g007740 33.424 368 214 13 40 388 92 447 2.59e-37 142
MS.gene020419.t1 MTR_1g111350 33.143 350 214 9 44 381 91 432 9.31e-37 140
MS.gene020419.t1 MTR_2g028190 32.990 388 191 18 46 385 55 421 1.49e-34 134
MS.gene020419.t1 MTR_3g053710 29.167 408 255 14 17 404 56 449 2.59e-33 130
MS.gene020419.t1 MTR_6g085010 31.627 332 201 12 63 381 138 456 1.05e-30 123
MS.gene020419.t1 MTR_8g103880 28.617 311 206 8 81 381 116 420 9.92e-29 117
MS.gene020419.t1 MTR_5g038360 30.189 371 235 14 29 383 38 400 2.25e-25 107
MS.gene020419.t1 MTR_0036s0070 31.290 310 178 12 78 369 87 379 1.08e-24 105
MS.gene020419.t1 MTR_2g059460 30.333 300 180 10 82 361 60 350 1.64e-24 104
MS.gene020419.t1 MTR_0036s0110 30.334 389 217 18 29 386 42 407 1.97e-24 105
MS.gene020419.t1 MTR_3g053980 28.101 395 246 15 9 381 70 448 5.79e-24 103
MS.gene020419.t1 MTR_3g028120 28.255 361 232 13 42 383 51 403 9.99e-24 103
MS.gene020419.t1 MTR_5g080890 31.098 328 190 16 80 384 85 399 5.71e-23 100
MS.gene020419.t1 MTR_2g059490 27.635 351 220 12 58 386 39 377 6.87e-23 100
MS.gene020419.t1 MTR_3g046300 29.446 343 213 9 69 384 85 425 2.81e-22 99.0
MS.gene020419.t1 MTR_8g096990 27.586 377 234 18 29 384 40 398 3.72e-22 98.2
MS.gene020419.t1 MTR_5g080960 30.488 328 192 15 80 384 85 399 4.39e-22 98.2
MS.gene020419.t1 MTR_0036s0050 30.887 327 192 10 78 382 87 401 2.54e-21 95.9
MS.gene020419.t1 MTR_5g012490 27.809 356 181 12 68 365 66 403 3.27e-21 95.9
MS.gene020419.t1 MTR_0024s0040 28.382 377 236 14 29 383 11 375 1.11e-19 90.9
MS.gene020419.t1 MTR_3g068000 30.323 310 183 14 84 367 76 378 1.71e-19 90.9
MS.gene020419.t1 MTR_5g044770 27.778 360 227 14 42 383 52 396 5.10e-19 89.0
MS.gene020419.t1 MTR_5g091130 30.058 346 208 12 69 384 179 520 1.60e-18 88.2
MS.gene020419.t1 MTR_8g038310 27.824 363 225 15 29 380 41 377 4.26e-18 86.3
MS.gene020419.t1 MTR_5g008150 27.606 355 213 14 53 382 40 375 7.52e-18 85.5
MS.gene020419.t1 MTR_5g008110 25.824 364 231 16 42 380 27 376 9.29e-18 85.1
MS.gene020419.t1 MTR_7g062360 31.111 315 178 15 84 367 62 368 1.11e-17 85.1
MS.gene020419.t1 MTR_1g060750 27.717 368 201 17 43 386 15 341 1.18e-17 84.3
MS.gene020419.t1 MTR_4g045857 30.631 222 129 7 81 283 58 273 1.26e-17 83.2
MS.gene020419.t1 MTR_2g055840 27.003 337 204 14 82 385 14 341 1.43e-17 84.3
MS.gene020419.t1 MTR_8g038280 27.356 329 206 14 78 383 83 401 1.58e-17 84.7
MS.gene020419.t1 MTR_4g100990 26.946 334 192 13 81 365 74 404 1.92e-17 84.7
MS.gene020419.t1 MTR_5g008070 29.573 328 195 15 78 383 78 391 2.05e-17 84.3
MS.gene020419.t1 MTR_4g095270 28.417 278 162 14 80 339 79 337 3.36e-16 81.3
MS.gene020419.t1 MTR_5g016260 29.179 329 188 19 80 382 87 396 5.18e-16 80.5
MS.gene020419.t1 MTR_4g095270 28.417 278 162 14 80 339 79 337 5.68e-16 80.5
MS.gene020419.t1 MTR_5g016260 29.179 329 188 19 80 382 87 396 6.34e-16 80.5
MS.gene020419.t1 MTR_2g067830 26.531 343 217 16 42 361 21 351 1.30e-15 78.6
MS.gene020419.t1 MTR_7g105850 26.870 361 206 17 68 382 75 423 6.57e-15 76.6
MS.gene020419.t1 MTR_5g077850 27.660 329 200 15 83 383 83 401 9.35e-15 76.3
MS.gene020419.t1 MTR_5g044570 26.649 379 211 19 29 383 39 374 1.65e-14 75.1
MS.gene020419.t1 MTR_7g080230 30.337 267 144 13 80 317 70 323 1.97e-14 75.5
MS.gene020419.t1 MTR_4g056420 28.228 333 198 16 80 380 76 399 2.98e-14 74.7
MS.gene020419.t1 MTR_2g084550 33.083 266 141 13 77 315 73 328 3.12e-14 74.7
MS.gene020419.t1 MTR_6g088760 23.747 379 254 16 42 398 52 417 3.27e-14 74.7
MS.gene020419.t1 MTR_5g077920 27.964 329 201 13 83 383 70 390 4.20e-14 74.3
MS.gene020419.t1 MTR_2g105300 28.938 273 165 13 109 361 66 329 1.09e-13 72.4
MS.gene020419.t1 MTR_7g021510 26.377 345 208 17 72 383 63 394 1.35e-13 72.8
MS.gene020419.t1 MTR_8g038250 25.606 371 214 15 34 382 44 374 1.62e-13 72.4
MS.gene020419.t1 MTR_4g109760 28.947 304 186 13 97 380 70 363 1.92e-12 68.9
MS.gene020419.t1 MTR_2g070430 23.512 336 214 13 82 380 88 417 4.03e-12 68.2
MS.gene020419.t1 MTR_1g078240 24.496 347 216 14 45 354 24 361 1.40e-11 66.2
MS.gene020419.t1 MTR_6g090290 27.744 328 207 15 80 386 85 403 1.54e-11 66.2
MS.gene020419.t1 MTR_1g078240 25.072 347 214 14 45 354 24 361 1.99e-11 65.9
MS.gene020419.t1 MTR_1g078240 25.072 347 214 14 45 354 24 361 2.40e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020419.t1 AT1G09750 60.891 404 148 8 8 401 26 429 6.47e-163 466
MS.gene020419.t1 AT3G54400 51.139 395 179 8 10 401 22 405 2.11e-124 367
MS.gene020419.t1 AT5G07030 48.000 400 194 7 8 400 42 434 4.33e-111 334
MS.gene020419.t1 AT1G01300 33.428 353 214 9 49 381 98 449 5.71e-39 146
MS.gene020419.t1 AT5G10770 30.120 415 245 14 17 404 60 456 1.40e-37 142
MS.gene020419.t1 AT3G18490 32.424 330 195 10 68 381 148 465 9.08e-37 140
MS.gene020419.t1 AT3G25700 30.518 367 218 11 50 383 52 414 2.51e-36 139
MS.gene020419.t1 AT2G03200 32.938 337 178 12 80 383 105 426 4.92e-36 138
MS.gene020419.t1 AT1G25510 34.161 322 195 9 69 381 135 448 1.68e-32 128
MS.gene020419.t1 AT3G61820 34.848 330 197 9 68 381 121 448 1.07e-31 126
MS.gene020419.t1 AT3G20015 30.328 366 231 9 41 386 79 440 1.68e-31 125
MS.gene020419.t1 AT1G79720 31.921 354 206 14 53 382 104 446 7.05e-30 121
MS.gene020419.t1 AT5G10760 33.671 395 204 20 17 381 63 429 1.82e-28 117
MS.gene020419.t1 AT5G45120 29.429 350 186 13 82 374 83 428 5.21e-23 101
MS.gene020419.t1 AT2G28040 26.590 346 207 12 53 380 28 344 1.45e-21 96.3
MS.gene020419.t1 AT2G28040 26.316 323 192 11 76 380 59 353 3.68e-21 95.1
MS.gene020419.t1 AT5G33340 26.748 329 210 10 78 385 86 404 1.34e-20 94.0
MS.gene020419.t1 AT3G52500 25.455 385 233 12 41 382 52 425 5.46e-19 89.4
MS.gene020419.t1 AT2G28010 25.648 347 210 12 53 380 37 354 1.26e-18 87.8
MS.gene020419.t1 AT2G39710 31.071 280 162 13 84 339 67 339 1.32e-18 87.8
MS.gene020419.t1 AT2G28030 25.857 321 195 10 77 380 56 350 2.12e-18 87.0
MS.gene020419.t1 AT5G02190 29.345 351 202 17 91 403 82 424 2.65e-18 87.0
MS.gene020419.t1 AT3G59080 27.451 357 226 10 58 384 146 499 1.69e-17 85.1
MS.gene020419.t1 AT2G42980 25.434 346 227 11 69 384 147 491 4.57e-17 83.6
MS.gene020419.t1 AT3G12700 26.441 295 194 10 41 317 65 354 6.64e-17 82.8
MS.gene020419.t1 AT1G64830 26.462 325 208 14 78 382 82 395 8.71e-17 82.4
MS.gene020419.t1 AT5G22850 27.378 347 195 19 46 354 46 373 2.65e-16 81.3
MS.gene020419.t1 AT1G31450 28.453 362 221 15 50 380 52 406 1.16e-15 79.0
MS.gene020419.t1 AT3G02740 28.731 268 157 10 76 320 4 260 2.55e-15 77.8
MS.gene020419.t1 AT3G02740 28.947 266 155 10 79 321 82 336 2.85e-15 78.2
MS.gene020419.t1 AT2G35615 26.555 418 232 20 11 381 20 409 3.20e-15 77.8
MS.gene020419.t1 AT1G66180 25.397 378 245 17 56 405 43 411 7.60e-15 76.6
MS.gene020419.t1 AT5G36260 24.302 358 224 13 41 363 28 373 8.24e-15 76.6
MS.gene020419.t1 AT1G08210 30.515 272 150 12 77 317 79 342 2.09e-14 75.5
MS.gene020419.t1 AT1G08210 30.515 272 150 12 77 317 113 376 2.23e-14 75.5
MS.gene020419.t1 AT1G08210 30.515 272 150 12 77 317 79 342 2.25e-14 75.1
MS.gene020419.t1 AT1G08210 30.515 272 150 12 77 317 127 390 2.39e-14 75.5
MS.gene020419.t1 AT1G05840 24.576 354 198 14 43 360 32 352 6.06e-13 70.9
MS.gene020419.t1 AT1G65240 26.415 265 155 11 78 317 70 319 6.71e-13 70.5
MS.gene020419.t1 AT2G36670 25.714 350 215 15 72 387 90 428 9.30e-13 70.5
MS.gene020419.t1 AT3G59080 27.697 343 207 8 58 384 146 463 9.75e-13 70.1
MS.gene020419.t1 AT3G50050 26.269 335 201 18 80 387 91 406 1.27e-12 70.1
MS.gene020419.t1 AT1G77480 28.022 364 196 16 61 383 44 382 2.20e-12 68.9
MS.gene020419.t1 AT3G25700 31.548 168 100 5 50 202 52 219 2.35e-12 68.2
MS.gene020419.t1 AT1G77480 29.041 365 191 18 61 383 44 382 3.30e-12 68.6
MS.gene020419.t1 AT2G36670 25.882 340 207 15 82 387 105 433 4.96e-12 68.2
MS.gene020419.t1 AT2G36670 25.882 340 207 15 82 387 34 362 7.30e-12 67.4
MS.gene020419.t1 AT2G36670 25.882 340 207 15 82 387 34 362 7.30e-12 67.4
MS.gene020419.t1 AT2G28225 24.625 333 180 14 82 381 67 361 7.56e-12 67.0
MS.gene020419.t1 AT4G16563 30.603 232 124 7 157 354 184 412 1.48e-11 66.6
MS.gene020419.t1 AT2G23945 24.412 340 216 16 40 354 53 376 3.69e-11 65.1

Find 124 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACATTGCATTTCACAGATT+TGG 0.209097 3.3:+82935221 MS.gene020419:CDS
AATTCCAGCCCAAGGACTTT+TGG 0.224408 3.3:+82934762 MS.gene020419:CDS
GCTCATTCTGGCTGGGTCTT+TGG 0.233936 3.3:-82934314 None:intergenic
GTTATCCCTAGCTACTCTTT+TGG 0.270512 3.3:+82934712 MS.gene020419:CDS
GTCGAACGCTAAAAGTTCTT+TGG 0.293009 3.3:-82935022 None:intergenic
AACCCGTTTACAACGCAATT+AGG 0.294497 3.3:+82935103 MS.gene020419:CDS
GGGCCGAAGAGACGGTCTTT+TGG 0.298389 3.3:-82934357 None:intergenic
TGGTGTGGTTCGAATGCTTT+TGG 0.298582 3.3:-82934917 None:intergenic
CTCTTCGGCCCCAATTGCTT+CGG 0.302831 3.3:+82934369 MS.gene020419:CDS
TCACCATGAGAAGAAATTAA+AGG 0.309724 3.3:+82936306 MS.gene020419:CDS
AAGGCCTGGCCCGAAGCAAT+TGG 0.338203 3.3:-82934379 None:intergenic
TTCACATGCAAAGGCTTTGT+TGG 0.341260 3.3:-82936366 None:intergenic
TCGAACGCTAAAAGTTCTTT+GGG 0.342499 3.3:-82935021 None:intergenic
ATCCCTAATTGCGTTGTAAA+CGG 0.345710 3.3:-82935106 None:intergenic
ATCAATGATGGTTCCGGATC+CGG 0.354629 3.3:-82935052 None:intergenic
GGCCCCAATTGCTTCGGGCC+AGG 0.355213 3.3:+82934375 MS.gene020419:CDS
AGTTTGATCCATAGCAGTTC+AGG 0.355328 3.3:+82935267 MS.gene020419:CDS
ACCCGTTTACAACGCAATTA+GGG 0.360383 3.3:+82935104 MS.gene020419:CDS
TCTTCGGCCCCAATTGCTTC+GGG 0.367155 3.3:+82934370 MS.gene020419:CDS
CCGAGTAAAGTGACCCGGTT+TGG 0.372002 3.3:-82934816 None:intergenic
GGTCTTTGGAGGCCATGTTT+AGG 0.373313 3.3:-82934300 None:intergenic
TCATGTCCAGCCACGGGCTC+TGG 0.378128 3.3:+82934607 MS.gene020419:CDS
GGATCCATCAATGCTATCTC+TGG 0.379282 3.3:+82934733 MS.gene020419:CDS
TACATAGTCCGTGTCAAAAT+CGG 0.383355 3.3:+82934415 MS.gene020419:CDS
AAATAGTAAGATTTGAAACT+TGG 0.389111 3.3:-82934859 None:intergenic
GTCCCTAGCTCTGGCTGCAC+TGG 0.389639 3.3:+82934493 MS.gene020419:CDS
CACATAACTGGTGGAGGCAT+TGG 0.393709 3.3:-82934539 None:intergenic
AGGCCTGGCCCGAAGCAATT+GGG 0.394921 3.3:-82934378 None:intergenic
CCAAACCGGGTCACTTTACT+CGG 0.396643 3.3:+82934816 MS.gene020419:CDS
TCCCTAATTGCGTTGTAAAC+GGG 0.400552 3.3:-82935105 None:intergenic
ACATTCCAATGGCACATAAC+TGG 0.407742 3.3:-82934551 None:intergenic
ATCCGGAACCATCATTGATT+CGG 0.413245 3.3:+82935056 MS.gene020419:CDS
AGAACCAGAGATAGCATTGA+TGG 0.414066 3.3:-82934737 None:intergenic
GCTCCTTTAATTTCTTCTCA+TGG 0.417149 3.3:-82936309 None:intergenic
ACCCGAATCAATGATGGTTC+CGG 0.417241 3.3:-82935058 None:intergenic
CATTCTGGCTGGGTCTTTGG+AGG 0.417382 3.3:-82934311 None:intergenic
TGTAAACGGGTTCCACAAAA+CGG 0.423278 3.3:-82935092 None:intergenic
ACCCGGTCAACTCCTCTTCA+TGG 0.424582 3.3:+82934441 MS.gene020419:CDS
CAACCAGTGCAGCCAGAGCT+AGG 0.427419 3.3:-82934496 None:intergenic
GTCCCAAGCCCAAAAGTCCT+TGG 0.427997 3.3:-82934771 None:intergenic
CGAGTAAAGTGACCCGGTTT+GGG 0.434912 3.3:-82934815 None:intergenic
TTATCTTTATTATCCCAAAC+CGG 0.435010 3.3:+82934802 MS.gene020419:CDS
AGAGGATAGATAGCTCATTC+TGG 0.435428 3.3:-82934326 None:intergenic
TCCGGAACCATCATTGATTC+GGG 0.439572 3.3:+82935057 MS.gene020419:CDS
TTCTTCCTCTTCACATGCAA+AGG 0.450301 3.3:-82936375 None:intergenic
GATAGATAGCTCATTCTGGC+TGG 0.450962 3.3:-82934322 None:intergenic
TCGGGCCAGGCCTTCAACAT+TGG 0.453690 3.3:+82934388 MS.gene020419:CDS
AAAGCTCCTAAGCTCGAAAA+AGG 0.458966 3.3:-82935153 None:intergenic
CATGCGCCAGAGCCCGTGGC+TGG 0.466461 3.3:-82934613 None:intergenic
ACATAACTGGTGGAGGCATT+GGG 0.467440 3.3:-82934538 None:intergenic
ACAGTGTAGTTCACATTGCT+TGG 0.476963 3.3:-82935321 None:intergenic
TATGTAGTTGCCAATGTTGA+AGG 0.481281 3.3:-82934398 None:intergenic
TCCTTCAACAAATCTTATGC+AGG 0.482523 3.3:+82934637 MS.gene020419:CDS
TGGACTCTCATGTCCAGCCA+CGG 0.483179 3.3:+82934600 MS.gene020419:CDS
ACGTTGAGATCGGAGTCATC+TGG 0.484141 3.3:-82934211 None:intergenic
ACCTGCATAAGATTTGTTGA+AGG 0.487641 3.3:-82934638 None:intergenic
GAGTTTAGAAAACAAGTAAC+CGG 0.488320 3.3:+82935129 MS.gene020419:CDS
TTAGCGTTCGACGTGAATAC+CGG 0.490806 3.3:+82935033 MS.gene020419:CDS
TTCCGTTCCACAATGTAGTC+AGG 0.496309 3.3:+82934573 MS.gene020419:CDS
TCCCAAGCCCAAAAGTCCTT+GGG 0.497011 3.3:-82934770 None:intergenic
GTAAACGGGTTCCACAAAAC+GGG 0.498348 3.3:-82935091 None:intergenic
ATCGTTGGCTTGTCTTGCAA+TGG 0.500484 3.3:+82935290 MS.gene020419:CDS
GTTTGGGATAATAAAGATAA+CGG 0.502287 3.3:-82934799 None:intergenic
TTTGGGATAATAAAGATAAC+GGG 0.503429 3.3:-82934798 None:intergenic
GTCACTTAAATTGGGACCCG+TGG 0.504362 3.3:+82934888 MS.gene020419:CDS
TATGAAAGACGTGGAAGATA+AGG 0.505378 3.3:+82936342 MS.gene020419:CDS
TTTGGATCTAAAATTACCAC+TGG 0.520013 3.3:+82935239 MS.gene020419:CDS
TGTGAAGAACTATGAAACAT+TGG 0.523272 3.3:+82935188 MS.gene020419:CDS
AAGCCCAAAAGTCCTTGGGC+TGG 0.523485 3.3:-82934766 None:intergenic
AGCCCAAAAGACCGTCTCTT+CGG 0.524317 3.3:+82934354 MS.gene020419:CDS
AACTGTACCCGAATCAATGA+TGG 0.524932 3.3:-82935064 None:intergenic
ATGGATCCAAAAGAGTAGCT+AGG 0.527831 3.3:-82934718 None:intergenic
AGATTCACTTAGATTAGCTA+CGG 0.529774 3.3:+82934687 MS.gene020419:CDS
CCATAGCAGTTCAGGATCGT+TGG 0.532599 3.3:+82935275 MS.gene020419:CDS
AACCAGTGCAGCCAGAGCTA+GGG 0.534147 3.3:-82934495 None:intergenic
GGCCTGGCCCGAAGCAATTG+GGG 0.536252 3.3:-82934377 None:intergenic
GGACTCTCATGTCCAGCCAC+GGG 0.536709 3.3:+82934601 MS.gene020419:CDS
TTCGACGTGAATACCGGATC+CGG 0.536848 3.3:+82935039 MS.gene020419:CDS
GACCATGAAGAGGAGTTGAC+CGG 0.537080 3.3:-82934443 None:intergenic
CCAACGATCCTGAACTGCTA+TGG 0.538186 3.3:-82935275 None:intergenic
AGCAATTGGGGCCGAAGAGA+CGG 0.539943 3.3:-82934365 None:intergenic
CTCCACCAGTTATGTGCCAT+TGG 0.544799 3.3:+82934546 MS.gene020419:CDS
GCTAAAAGTTCTTTGGGGAA+AGG 0.546793 3.3:-82935015 None:intergenic
TTTGAAACTTGGAAGGCAGT+AGG 0.547376 3.3:-82934848 None:intergenic
AGTAAGATTTGAAACTTGGA+AGG 0.547857 3.3:-82934855 None:intergenic
AGTTGCCAATGTTGAAGGCC+TGG 0.547921 3.3:-82934393 None:intergenic
CAATGGCACATAACTGGTGG+AGG 0.558511 3.3:-82934545 None:intergenic
GGAACATGCGCCAGAGCCCG+TGG 0.560056 3.3:-82934617 None:intergenic
GGTTCTTCAATTCCAGCCCA+AGG 0.560164 3.3:+82934754 MS.gene020419:CDS
CGACGAGGATTGCGAAGAAG+TGG 0.573330 3.3:-82934937 None:intergenic
TGGATCCAAAAGAGTAGCTA+GGG 0.577281 3.3:-82934717 None:intergenic
CAATTTAACCGGAATTACCG+TGG 0.578946 3.3:+82934978 MS.gene020419:CDS
ATAGATAGCTCATTCTGGCT+GGG 0.581666 3.3:-82934321 None:intergenic
TATCTTTATTATCCCAAACC+GGG 0.590415 3.3:+82934803 MS.gene020419:CDS
GTGAAATGCAATGTAATAGT+TGG 0.590687 3.3:-82935213 None:intergenic
CATGGGAATTACGTTGAGAT+CGG 0.592922 3.3:-82934221 None:intergenic
AATTTAACCGGAATTACCGT+GGG 0.594540 3.3:+82934979 MS.gene020419:CDS
CTCAACGTAATTCCCATGTA+CGG 0.595179 3.3:+82934226 MS.gene020419:CDS
CGTGTCAAAATCGGAACACC+CGG 0.597259 3.3:+82934424 MS.gene020419:CDS
TGGCTGGACATGAGAGTCCA+CGG 0.597889 3.3:-82934597 None:intergenic
GGGTCCCAATTTAAGTGACC+CGG 0.609037 3.3:-82934884 None:intergenic
GACCTGACTACATTGTGGAA+CGG 0.611603 3.3:-82934575 None:intergenic
ACCATGAAGAGGAGTTGACC+GGG 0.612103 3.3:-82934442 None:intergenic
TTCCAATGGCACATAACTGG+TGG 0.614155 3.3:-82934548 None:intergenic
CCTCGTCGGTACTTGTGTCA+AGG 0.618851 3.3:-82934465 None:intergenic
AAGTGAATCTTGAACAAGTG+TGG 0.620682 3.3:-82934674 None:intergenic
TCACTTAAATTGGGACCCGT+GGG 0.630294 3.3:+82934889 MS.gene020419:CDS
TTGTGTCAAGGACCATGAAG+AGG 0.644346 3.3:-82934453 None:intergenic
GCTAGGGACAAAAGCCTCGT+CGG 0.645348 3.3:-82934479 None:intergenic
CCACGGACCTGACTACATTG+TGG 0.652854 3.3:-82934580 None:intergenic
AAATTGACAAAATAAAGAGA+TGG 0.653235 3.3:-82934961 None:intergenic
TGTGGAACGGAACATTCCAA+TGG 0.656650 3.3:-82934562 None:intergenic
TACACCCGAGTAAAGTGACC+CGG 0.661103 3.3:-82934821 None:intergenic
TGGATCAAACTATTCTCCAG+TGG 0.664018 3.3:-82935255 None:intergenic
CCACAATGTAGTCAGGTCCG+TGG 0.670073 3.3:+82934580 MS.gene020419:CDS
AGGATTGCGAAGAAGTGGTG+TGG 0.674358 3.3:-82934932 None:intergenic
CGAACGCTAAAAGTTCTTTG+GGG 0.675408 3.3:-82935020 None:intergenic
TCATCTGGTTGAGATGCACA+TGG 0.677104 3.3:-82934196 None:intergenic
TTGAGATGCACATGGATCAG+TGG 0.680155 3.3:-82934188 None:intergenic
CAAACCGGGTCACTTTACTC+GGG 0.687401 3.3:+82934817 MS.gene020419:CDS
GTGTCACATTATGAAAGACG+TGG 0.695541 3.3:+82936333 MS.gene020419:CDS
AAATAAAGAGATGGACGACG+AGG 0.698074 3.3:-82934952 None:intergenic
CCTTGACACAAGTACCGACG+AGG 0.718459 3.3:+82934465 MS.gene020419:CDS
GGACAATAGAGTCCTAAACA+TGG 0.719535 3.3:+82934288 MS.gene020419:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCACTAAAAAAATATTAAAT+TGG + chr3.3:82936236-82936255 MS.gene020419:intron 10.0%
!!! TATTATTGCTATTATTATTA+TGG + chr3.3:82935869-82935888 MS.gene020419:intron 10.0%
!!! TAATAATTTAGTTCTTTTGT+CGG - chr3.3:82935907-82935926 None:intergenic 15.0%
!! AAATTGACAAAATAAAGAGA+TGG - chr3.3:82934964-82934983 None:intergenic 20.0%
!! CAAGATTACAAGAAAAAAAA+TGG - chr3.3:82936197-82936216 None:intergenic 20.0%
!! TAATACAAAGATTGATTACT+TGG - chr3.3:82935854-82935873 None:intergenic 20.0%
!!! AAATAGTAAGATTTGAAACT+TGG - chr3.3:82934862-82934881 None:intergenic 20.0%
!!! CTTTATTTTGTCAATTTAAC+CGG + chr3.3:82934967-82934986 MS.gene020419:CDS 20.0%
!!! TCCTTTTGTTTATTTATGAA+CGG + chr3.3:82935748-82935767 MS.gene020419:intron 20.0%
!!! TTCAAATCTTACTATTTTTC+CGG + chr3.3:82934865-82934884 MS.gene020419:CDS 20.0%
!!! TTTCTCTAAAAACATAAAAC+AGG - chr3.3:82936161-82936180 None:intergenic 20.0%
! AAGAAAAGAGTTTCTCATTA+CGG - chr3.3:82936023-82936042 None:intergenic 25.0%
! AATACCAACCTTATTATTCA+CGG - chr3.3:82935400-82935419 None:intergenic 25.0%
! ATACATAAGGCAATTTCTTA+GGG - chr3.3:82935695-82935714 None:intergenic 25.0%
! ATCGTAATTCATCATACATA+AGG - chr3.3:82935708-82935727 None:intergenic 25.0%
! ATTATCAACAACAGAATTTG+AGG + chr3.3:82935361-82935380 MS.gene020419:intron 25.0%
! TTATCAACAACAGAATTTGA+GGG + chr3.3:82935362-82935381 MS.gene020419:intron 25.0%
! TTATCTTTATTATCCCAAAC+CGG + chr3.3:82934802-82934821 MS.gene020419:CDS 25.0%
! TTTATTTATGAACGGGAATA+AGG + chr3.3:82935756-82935775 MS.gene020419:intron 25.0%
!! GTTTGGGATAATAAAGATAA+CGG - chr3.3:82934802-82934821 None:intergenic 25.0%
!! TTTGGGATAATAAAGATAAC+GGG - chr3.3:82934801-82934820 None:intergenic 25.0%
!!! AAATAGTTGAACTTTTGAGA+TGG - chr3.3:82936048-82936067 None:intergenic 25.0%
!!! CCTTTTGTTTATTTATGAAC+GGG + chr3.3:82935749-82935768 MS.gene020419:intron 25.0%
!!! GATCATTTTGATTATCCAAT+TGG + chr3.3:82935635-82935654 MS.gene020419:intron 25.0%
!!! GTTATGCTTTTGTTTTAGTT+TGG + chr3.3:82935487-82935506 MS.gene020419:intron 25.0%
!!! TCAAATCTTACTATTTTTCC+GGG + chr3.3:82934866-82934885 MS.gene020419:CDS 25.0%
!!! TTTTTTTAGTGAAGCATCAA+TGG - chr3.3:82936228-82936247 None:intergenic 25.0%
AAAAGGAAAATTCCATTCAC+TGG - chr3.3:82935735-82935754 None:intergenic 30.0%
AAAGGAAAATTCCATTCACT+GGG - chr3.3:82935734-82935753 None:intergenic 30.0%
CATACATAAGGCAATTTCTT+AGG - chr3.3:82935696-82935715 None:intergenic 30.0%
CCCGTTCATAAATAAACAAA+AGG - chr3.3:82935752-82935771 None:intergenic 30.0%
GAGTTTAGAAAACAAGTAAC+CGG + chr3.3:82935129-82935148 MS.gene020419:CDS 30.0%
GTGAAATGCAATGTAATAGT+TGG - chr3.3:82935216-82935235 None:intergenic 30.0%
GTTTGATACCGTGAATAATA+AGG + chr3.3:82935389-82935408 MS.gene020419:intron 30.0%
TACATAAGGCAATTTCTTAG+GGG - chr3.3:82935694-82935713 None:intergenic 30.0%
TACATTGCATTTCACAGATT+TGG + chr3.3:82935221-82935240 MS.gene020419:CDS 30.0%
TATCTTTATTATCCCAAACC+GGG + chr3.3:82934803-82934822 MS.gene020419:CDS 30.0%
TCACCATGAGAAGAAATTAA+AGG + chr3.3:82936306-82936325 MS.gene020419:CDS 30.0%
TCCGTTTGAAAATGTCAAAT+CGG - chr3.3:82935985-82936004 None:intergenic 30.0%
TGTGAAGAACTATGAAACAT+TGG + chr3.3:82935188-82935207 MS.gene020419:CDS 30.0%
TTTGAAAATGTCAAATCGGT+AGG - chr3.3:82935981-82936000 None:intergenic 30.0%
TTTGTGAGATCATCACAAAT+TGG - chr3.3:82936105-82936124 None:intergenic 30.0%
! AGATTCACTTAGATTAGCTA+CGG + chr3.3:82934687-82934706 MS.gene020419:CDS 30.0%
! AGTAAGATTTGAAACTTGGA+AGG - chr3.3:82934858-82934877 None:intergenic 30.0%
! AGTTATTTCAGCAATCCAAT+TGG - chr3.3:82935653-82935672 None:intergenic 30.0%
! CAAATTATGAACTTCTGGAA+TGG - chr3.3:82936135-82936154 None:intergenic 30.0%
! TACTTGTGTTTAAGTTCGTA+TGG - chr3.3:82935941-82935960 None:intergenic 30.0%
! TTTGGATCTAAAATTACCAC+TGG + chr3.3:82935239-82935258 MS.gene020419:CDS 30.0%
!! TTTGGATGTTTTTGTTGTAG+TGG + chr3.3:82935505-82935524 MS.gene020419:intron 30.0%
!!! ACCGATTTGACATTTTCAAA+CGG + chr3.3:82935981-82936000 MS.gene020419:intron 30.0%
AAGTGAATCTTGAACAAGTG+TGG - chr3.3:82934677-82934696 None:intergenic 35.0%
AATTTAACCGGAATTACCGT+GGG + chr3.3:82934979-82934998 MS.gene020419:CDS 35.0%
ACATAAGGCAATTTCTTAGG+GGG - chr3.3:82935693-82935712 None:intergenic 35.0%
AGTTCAACTATTTGCATGCA+TGG + chr3.3:82936055-82936074 MS.gene020419:intron 35.0%
ATCCCTAATTGCGTTGTAAA+CGG - chr3.3:82935109-82935128 None:intergenic 35.0%
ATTGTGATCCTTAGCACAAA+GGG + chr3.3:82935607-82935626 MS.gene020419:intron 35.0%
GAATTTCTTCTTTAGCCACA+TGG - chr3.3:82935548-82935567 None:intergenic 35.0%
GATACCGTGAATAATAAGGT+TGG + chr3.3:82935393-82935412 MS.gene020419:intron 35.0%
GGTGACAAATTATGAACTTC+TGG - chr3.3:82936140-82936159 None:intergenic 35.0%
TACATAGTCCGTGTCAAAAT+CGG + chr3.3:82934415-82934434 MS.gene020419:CDS 35.0%
TATGAAAGACGTGGAAGATA+AGG + chr3.3:82936342-82936361 MS.gene020419:CDS 35.0%
TATGTAGTTGCCAATGTTGA+AGG - chr3.3:82934401-82934420 None:intergenic 35.0%
TCCTTCAACAAATCTTATGC+AGG + chr3.3:82934637-82934656 MS.gene020419:CDS 35.0%
TCGAACGCTAAAAGTTCTTT+GGG - chr3.3:82935024-82935043 None:intergenic 35.0%
TTTGTAACTAGCTAGCTAGA+AGG + chr3.3:82935430-82935449 MS.gene020419:intron 35.0%
! GCTCCTTTAATTTCTTCTCA+TGG - chr3.3:82936312-82936331 None:intergenic 35.0%
! TACAGTTATTACCCGTTTTG+TGG + chr3.3:82935080-82935099 MS.gene020419:CDS 35.0%
! TTTTCCGGGTCACTTAAATT+GGG + chr3.3:82934880-82934899 MS.gene020419:CDS 35.0%
! TTTTTCCGGGTCACTTAAAT+TGG + chr3.3:82934879-82934898 MS.gene020419:CDS 35.0%
!! ACCTGCATAAGATTTGTTGA+AGG - chr3.3:82934641-82934660 None:intergenic 35.0%
!! AGGTACATTTACTTTTCCCA+CGG - chr3.3:82934998-82935017 None:intergenic 35.0%
!!! TCTGTTTTTTGTGGGTTGAA+TGG - chr3.3:82934262-82934281 None:intergenic 35.0%
AAAGCTCCTAAGCTCGAAAA+AGG - chr3.3:82935156-82935175 None:intergenic 40.0%
AAATAAAGAGATGGACGACG+AGG - chr3.3:82934955-82934974 None:intergenic 40.0%
AACCCGTTTACAACGCAATT+AGG + chr3.3:82935103-82935122 MS.gene020419:CDS 40.0%
AACTGTACCCGAATCAATGA+TGG - chr3.3:82935067-82935086 None:intergenic 40.0%
ACAGTGTAGTTCACATTGCT+TGG - chr3.3:82935324-82935343 None:intergenic 40.0%
ACATTCCAATGGCACATAAC+TGG - chr3.3:82934554-82934573 None:intergenic 40.0%
ACCCACAAAAAACAGACTCA+TGG + chr3.3:82934266-82934285 MS.gene020419:CDS 40.0%
ACCCGTTTACAACGCAATTA+GGG + chr3.3:82935104-82935123 MS.gene020419:CDS 40.0%
AGAGGATAGATAGCTCATTC+TGG - chr3.3:82934329-82934348 None:intergenic 40.0%
AGTTTGATCCATAGCAGTTC+AGG + chr3.3:82935267-82935286 MS.gene020419:CDS 40.0%
ATGGATCCAAAAGAGTAGCT+AGG - chr3.3:82934721-82934740 None:intergenic 40.0%
ATTACGATTGTCCCAGTGAA+TGG + chr3.3:82935720-82935739 MS.gene020419:intron 40.0%
CAATTTAACCGGAATTACCG+TGG + chr3.3:82934978-82934997 MS.gene020419:CDS 40.0%
CATGGGAATTACGTTGAGAT+CGG - chr3.3:82934224-82934243 None:intergenic 40.0%
CCCACAAAAAACAGACTCAT+GGG + chr3.3:82934267-82934286 MS.gene020419:CDS 40.0%
CGAACGCTAAAAGTTCTTTG+GGG - chr3.3:82935023-82935042 None:intergenic 40.0%
CTAGCTAGTTACAAAGCTCA+CGG - chr3.3:82935425-82935444 None:intergenic 40.0%
CTCAACGTAATTCCCATGTA+CGG + chr3.3:82934226-82934245 MS.gene020419:CDS 40.0%
CTGGATTCCAATTCCATTCA+CGG - chr3.3:82935787-82935806 None:intergenic 40.0%
GAAAGACAAAAACAACACGC+TGG - chr3.3:82935806-82935825 None:intergenic 40.0%
GATTGTGATCCTTAGCACAA+AGG + chr3.3:82935606-82935625 MS.gene020419:intron 40.0%
GGACAATAGAGTCCTAAACA+TGG + chr3.3:82934288-82934307 MS.gene020419:CDS 40.0%
GTCGAACGCTAAAAGTTCTT+TGG - chr3.3:82935025-82935044 None:intergenic 40.0%
GTGTCACATTATGAAAGACG+TGG + chr3.3:82936333-82936352 MS.gene020419:CDS 40.0%
TAGCTAGTTACAAAGCTCAC+GGG - chr3.3:82935424-82935443 None:intergenic 40.0%
TCAACTATTTGCATGCATGG+AGG + chr3.3:82936058-82936077 MS.gene020419:intron 40.0%
TCCCTAATTGCGTTGTAAAC+GGG - chr3.3:82935108-82935127 None:intergenic 40.0%
TGGATCAAACTATTCTCCAG+TGG - chr3.3:82935258-82935277 None:intergenic 40.0%
TGGATCCAAAAGAGTAGCTA+GGG - chr3.3:82934720-82934739 None:intergenic 40.0%
TGGATTCCAATTCCATTCAC+GGG - chr3.3:82935786-82935805 None:intergenic 40.0%
TTGTGATCCTTAGCACAAAG+GGG + chr3.3:82935608-82935627 MS.gene020419:intron 40.0%
! AGAACCAGAGATAGCATTGA+TGG - chr3.3:82934740-82934759 None:intergenic 40.0%
! ATAGATAGCTCATTCTGGCT+GGG - chr3.3:82934324-82934343 None:intergenic 40.0%
! ATCCGGAACCATCATTGATT+CGG + chr3.3:82935056-82935075 MS.gene020419:CDS 40.0%
! GCTAAAAGTTCTTTGGGGAA+AGG - chr3.3:82935018-82935037 None:intergenic 40.0%
! GTTATCCCTAGCTACTCTTT+TGG + chr3.3:82934712-82934731 MS.gene020419:CDS 40.0%
! TACTTTTCCCACGGTAATTC+CGG - chr3.3:82934989-82935008 None:intergenic 40.0%
! TGTAAACGGGTTCCACAAAA+CGG - chr3.3:82935095-82935114 None:intergenic 40.0%
! TTTGAAACTTGGAAGGCAGT+AGG - chr3.3:82934851-82934870 None:intergenic 40.0%
!! TGTTTTTGTTGTAGTGGCCT+AGG + chr3.3:82935511-82935530 MS.gene020419:intron 40.0%
!!! CCCATGAGTCTGTTTTTTGT+GGG - chr3.3:82934270-82934289 None:intergenic 40.0%
!!! TCCCATGAGTCTGTTTTTTG+TGG - chr3.3:82934271-82934290 None:intergenic 40.0%
ACCCGAATCAATGATGGTTC+CGG - chr3.3:82935061-82935080 None:intergenic 45.0%
ATCAATGATGGTTCCGGATC+CGG - chr3.3:82935055-82935074 None:intergenic 45.0%
ATCGTTACCCCTTTGTGCTA+AGG - chr3.3:82935618-82935637 None:intergenic 45.0%
GAATAAGGCATACCCGTGAA+TGG + chr3.3:82935771-82935790 MS.gene020419:intron 45.0%
GACCTGACTACATTGTGGAA+CGG - chr3.3:82934578-82934597 None:intergenic 45.0%
GATAGATAGCTCATTCTGGC+TGG - chr3.3:82934325-82934344 None:intergenic 45.0%
GGATCCATCAATGCTATCTC+TGG + chr3.3:82934733-82934752 MS.gene020419:CDS 45.0%
TCACTTAAATTGGGACCCGT+GGG + chr3.3:82934889-82934908 MS.gene020419:CDS 45.0%
TCATCTGGTTGAGATGCACA+TGG - chr3.3:82934199-82934218 None:intergenic 45.0%
TCCTAAGCTCGAAAAAGGTC+CGG - chr3.3:82935151-82935170 None:intergenic 45.0%
TGTAGTGGCCTAGGTGAAAA+GGG + chr3.3:82935520-82935539 MS.gene020419:intron 45.0%
TGTGGAACGGAACATTCCAA+TGG - chr3.3:82934565-82934584 None:intergenic 45.0%
TTAGCGTTCGACGTGAATAC+CGG + chr3.3:82935033-82935052 MS.gene020419:CDS 45.0%
TTCAGACAACGTGATTGCAC+TGG + chr3.3:82935823-82935842 MS.gene020419:intron 45.0%
TTCCAATGGCACATAACTGG+TGG - chr3.3:82934551-82934570 None:intergenic 45.0%
TTCCGTTCCACAATGTAGTC+AGG + chr3.3:82934573-82934592 MS.gene020419:CDS 45.0%
TTGAGATGCACATGGATCAG+TGG - chr3.3:82934191-82934210 None:intergenic 45.0%
TTGTAGTGGCCTAGGTGAAA+AGG + chr3.3:82935519-82935538 MS.gene020419:intron 45.0%
TTGTGTCAAGGACCATGAAG+AGG - chr3.3:82934456-82934475 None:intergenic 45.0%
! AATTCCAGCCCAAGGACTTT+TGG + chr3.3:82934762-82934781 MS.gene020419:CDS 45.0%
! ACATGGTACCCTTTTCACCT+AGG - chr3.3:82935531-82935550 None:intergenic 45.0%
! ATTCCAGCCCAAGGACTTTT+GGG + chr3.3:82934763-82934782 MS.gene020419:CDS 45.0%
! GGTGAGCATTTTCCGTACAT+GGG - chr3.3:82934241-82934260 None:intergenic 45.0%
! GGTGTGGTTCGAATGCTTTT+GGG - chr3.3:82934919-82934938 None:intergenic 45.0%
! GTAAACGGGTTCCACAAAAC+GGG - chr3.3:82935094-82935113 None:intergenic 45.0%
! TGGTGAGCATTTTCCGTACA+TGG - chr3.3:82934242-82934261 None:intergenic 45.0%
! TGGTGTGGTTCGAATGCTTT+TGG - chr3.3:82934920-82934939 None:intergenic 45.0%
!! ACATAACTGGTGGAGGCATT+GGG - chr3.3:82934541-82934560 None:intergenic 45.0%
!! ATCGTTGGCTTGTCTTGCAA+TGG + chr3.3:82935290-82935309 MS.gene020419:CDS 45.0%
!! TAGGTGAAAAGGGTACCATG+TGG + chr3.3:82935530-82935549 MS.gene020419:intron 45.0%
!! TCCGGAACCATCATTGATTC+GGG + chr3.3:82935057-82935076 MS.gene020419:CDS 45.0%
!!! AATGCTTTTGGGTTGACCCA+CGG - chr3.3:82934908-82934927 None:intergenic 45.0%
!!! GGTCTTTTGGGCTAGTAAAG+AGG - chr3.3:82934347-82934366 None:intergenic 45.0%
ACCATGAAGAGGAGTTGACC+GGG - chr3.3:82934445-82934464 None:intergenic 50.0%
ACGTTGAGATCGGAGTCATC+TGG - chr3.3:82934214-82934233 None:intergenic 50.0%
AGCCCAAAAGACCGTCTCTT+CGG + chr3.3:82934354-82934373 MS.gene020419:CDS 50.0%
AGGATTGCGAAGAAGTGGTG+TGG - chr3.3:82934935-82934954 None:intergenic 50.0%
AGTTGCCAATGTTGAAGGCC+TGG - chr3.3:82934396-82934415 None:intergenic 50.0%
CAATGGCACATAACTGGTGG+AGG - chr3.3:82934548-82934567 None:intergenic 50.0%
CCAACGATCCTGAACTGCTA+TGG - chr3.3:82935278-82935297 None:intergenic 50.0%
CCATAGCAGTTCAGGATCGT+TGG + chr3.3:82935275-82935294 MS.gene020419:CDS 50.0%
CGAGTAAAGTGACCCGGTTT+GGG - chr3.3:82934818-82934837 None:intergenic 50.0%
CGTGTCAAAATCGGAACACC+CGG + chr3.3:82934424-82934443 MS.gene020419:CDS 50.0%
CTCCACCAGTTATGTGCCAT+TGG + chr3.3:82934546-82934565 MS.gene020419:CDS 50.0%
GACCATGAAGAGGAGTTGAC+CGG - chr3.3:82934446-82934465 None:intergenic 50.0%
GGCATACCCGTGAATGGAAT+TGG + chr3.3:82935777-82935796 MS.gene020419:intron 50.0%
GGGTCCCAATTTAAGTGACC+CGG - chr3.3:82934887-82934906 None:intergenic 50.0%
GGTTCTTCAATTCCAGCCCA+AGG + chr3.3:82934754-82934773 MS.gene020419:CDS 50.0%
GTCACTTAAATTGGGACCCG+TGG + chr3.3:82934888-82934907 MS.gene020419:CDS 50.0%
TACACCCGAGTAAAGTGACC+CGG - chr3.3:82934824-82934843 None:intergenic 50.0%
TCCCAAGCCCAAAAGTCCTT+GGG - chr3.3:82934773-82934792 None:intergenic 50.0%
TTCGACGTGAATACCGGATC+CGG + chr3.3:82935039-82935058 MS.gene020419:CDS 50.0%
! CAAACCGGGTCACTTTACTC+GGG + chr3.3:82934817-82934836 MS.gene020419:CDS 50.0%
! CACATAACTGGTGGAGGCAT+TGG - chr3.3:82934542-82934561 None:intergenic 50.0%
! CCAAACCGGGTCACTTTACT+CGG + chr3.3:82934816-82934835 MS.gene020419:CDS 50.0%
! GGTCTTTGGAGGCCATGTTT+AGG - chr3.3:82934303-82934322 None:intergenic 50.0%
!! CGGGTGTTCCGATTTTGACA+CGG - chr3.3:82934426-82934445 None:intergenic 50.0%
!!! ACCGGACCTTTTTCGAGCTT+AGG + chr3.3:82935147-82935166 MS.gene020419:CDS 50.0%
!!! ATGCTTTTGGGTTGACCCAC+GGG - chr3.3:82934907-82934926 None:intergenic 50.0%
AACCAGTGCAGCCAGAGCTA+GGG - chr3.3:82934498-82934517 None:intergenic 55.0%
AAGCCCAAAAGTCCTTGGGC+TGG - chr3.3:82934769-82934788 None:intergenic 55.0%
ACCCGGTCAACTCCTCTTCA+TGG + chr3.3:82934441-82934460 MS.gene020419:CDS 55.0%
AGCAATTGGGGCCGAAGAGA+CGG - chr3.3:82934368-82934387 None:intergenic 55.0%
CCACAATGTAGTCAGGTCCG+TGG + chr3.3:82934580-82934599 MS.gene020419:CDS 55.0%
CCACGGACCTGACTACATTG+TGG - chr3.3:82934583-82934602 None:intergenic 55.0%
CCGAGTAAAGTGACCCGGTT+TGG - chr3.3:82934819-82934838 None:intergenic 55.0%
CCTCGTCGGTACTTGTGTCA+AGG - chr3.3:82934468-82934487 None:intergenic 55.0%
CGACGAGGATTGCGAAGAAG+TGG - chr3.3:82934940-82934959 None:intergenic 55.0%
CTCTTCGGCCCCAATTGCTT+CGG + chr3.3:82934369-82934388 MS.gene020419:CDS 55.0%
GCTAGGGACAAAAGCCTCGT+CGG - chr3.3:82934482-82934501 None:intergenic 55.0%
GTCCCAAGCCCAAAAGTCCT+TGG - chr3.3:82934774-82934793 None:intergenic 55.0%
TCTTCGGCCCCAATTGCTTC+GGG + chr3.3:82934370-82934389 MS.gene020419:CDS 55.0%
TGGACTCTCATGTCCAGCCA+CGG + chr3.3:82934600-82934619 MS.gene020419:CDS 55.0%
TGGCTGGACATGAGAGTCCA+CGG - chr3.3:82934600-82934619 None:intergenic 55.0%
! AGCCCAAGGACTTTTGGGCT+TGG + chr3.3:82934768-82934787 MS.gene020419:CDS 55.0%
! CATTCTGGCTGGGTCTTTGG+AGG - chr3.3:82934314-82934333 None:intergenic 55.0%
! GAGGCTTTTGTCCCTAGCTC+TGG + chr3.3:82934484-82934503 MS.gene020419:CDS 55.0%
! GCCCAAGGACTTTTGGGCTT+GGG + chr3.3:82934769-82934788 MS.gene020419:CDS 55.0%
! GCTCATTCTGGCTGGGTCTT+TGG - chr3.3:82934317-82934336 None:intergenic 55.0%
! GGCCGAAGAGACGGTCTTTT+GGG - chr3.3:82934359-82934378 None:intergenic 55.0%
!! CCTTGACACAAGTACCGACG+AGG + chr3.3:82934465-82934484 MS.gene020419:CDS 55.0%
AAGGCCTGGCCCGAAGCAAT+TGG - chr3.3:82934382-82934401 None:intergenic 60.0%
AGGCCTGGCCCGAAGCAATT+GGG - chr3.3:82934381-82934400 None:intergenic 60.0%
CAACCAGTGCAGCCAGAGCT+AGG - chr3.3:82934499-82934518 None:intergenic 60.0%
GGACTCTCATGTCCAGCCAC+GGG + chr3.3:82934601-82934620 MS.gene020419:CDS 60.0%
TCGGGCCAGGCCTTCAACAT+TGG + chr3.3:82934388-82934407 MS.gene020419:CDS 60.0%
! GGACTTTTGGGCTTGGGACG+TGG + chr3.3:82934775-82934794 MS.gene020419:CDS 60.0%
! GGGCCGAAGAGACGGTCTTT+TGG - chr3.3:82934360-82934379 None:intergenic 60.0%
GGCCTGGCCCGAAGCAATTG+GGG - chr3.3:82934380-82934399 None:intergenic 65.0%
TCATGTCCAGCCACGGGCTC+TGG + chr3.3:82934607-82934626 MS.gene020419:CDS 65.0%
!! GTCCCTAGCTCTGGCTGCAC+TGG + chr3.3:82934493-82934512 MS.gene020419:CDS 65.0%
GGAACATGCGCCAGAGCCCG+TGG - chr3.3:82934620-82934639 None:intergenic 70.0%
GGCCCCAATTGCTTCGGGCC+AGG + chr3.3:82934375-82934394 MS.gene020419:CDS 70.0%
CATGCGCCAGAGCCCGTGGC+TGG - chr3.3:82934616-82934635 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 82934172 82936387 82934172 ID=MS.gene020419
chr3.3 mRNA 82934172 82936387 82934172 ID=MS.gene020419.t1;Parent=MS.gene020419
chr3.3 exon 82934172 82935329 82934172 ID=MS.gene020419.t1.exon1;Parent=MS.gene020419.t1
chr3.3 CDS 82934172 82935329 82934172 ID=cds.MS.gene020419.t1;Parent=MS.gene020419.t1
chr3.3 exon 82936274 82936387 82936274 ID=MS.gene020419.t1.exon2;Parent=MS.gene020419.t1
chr3.3 CDS 82936274 82936387 82936274 ID=cds.MS.gene020419.t1;Parent=MS.gene020419.t1
Gene Sequence

>MS.gene020419

ATGAGCATGAGCAATGCCACTGATCCATGTGCATCTCAACCAGATGACTCCGATCTCAACGTAATTCCCATGTACGGAAAATGCTCACCATTCAACCCACAAAAAACAGACTCATGGGACAATAGAGTCCTAAACATGGCCTCCAAAGACCCAGCCAGAATGAGCTATCTATCCTCTTTACTAGCCCAAAAGACCGTCTCTTCGGCCCCAATTGCTTCGGGCCAGGCCTTCAACATTGGCAACTACATAGTCCGTGTCAAAATCGGAACACCCGGTCAACTCCTCTTCATGGTCCTTGACACAAGTACCGACGAGGCTTTTGTCCCTAGCTCTGGCTGCACTGGTTGCTCTGCAACAACTTTCTTACCCAATGCCTCCACCAGTTATGTGCCATTGGAATGTTCCGTTCCACAATGTAGTCAGGTCCGTGGACTCTCATGTCCAGCCACGGGCTCTGGCGCATGTTCCTTCAACAAATCTTATGCAGGTTCAACTTATTCTGCCACACTTGTTCAAGATTCACTTAGATTAGCTACGGATGTTATCCCTAGCTACTCTTTTGGATCCATCAATGCTATCTCTGGTTCTTCAATTCCAGCCCAAGGACTTTTGGGCTTGGGACGTGGCCCGTTATCTTTATTATCCCAAACCGGGTCACTTTACTCGGGTGTATTCTCCTACTGCCTTCCAAGTTTCAAATCTTACTATTTTTCCGGGTCACTTAAATTGGGACCCGTGGGTCAACCCAAAAGCATTCGAACCACACCACTTCTTCGCAATCCTCGTCGTCCATCTCTTTATTTTGTCAATTTAACCGGAATTACCGTGGGAAAAGTAAATGTACCTTTCCCCAAAGAACTTTTAGCGTTCGACGTGAATACCGGATCCGGAACCATCATTGATTCGGGTACAGTTATTACCCGTTTTGTGGAACCCGTTTACAACGCAATTAGGGATGAGTTTAGAAAACAAGTAACCGGACCTTTTTCGAGCTTAGGAGCTTTCGACACATGTTTTGTGAAGAACTATGAAACATTGGCACCAACTATTACATTGCATTTCACAGATTTGGATCTAAAATTACCACTGGAGAATAGTTTGATCCATAGCAGTTCAGGATCGTTGGCTTGTCTTGCAATGGCGTCTGCACCAAGCAATGTGAACTACACTGTGTTGAACGTGATTGCTAATTATCAACAACAGAATTTGAGGGTTTTGTTTGATACCGTGAATAATAAGGTTGGTATTGCCCGTGAGCTTTGTAACTAGCTAGCTAGAAGGTAGCTAGCTACATTTTAATTTGTTATTTAGCTAAGTTATGCTTTTGTTTTAGTTTGGATGTTTTTGTTGTAGTGGCCTAGGTGAAAAGGGTACCATGTGGCTAAAGAAGAAATTCAATTAATAATGCATGTAAAATGTCGTCTTTATTAGTGTGATTGTGATCCTTAGCACAAAGGGGTAACGATCATTTTGATTATCCAATTGGATTGCTGAAATAACTTTTGCTGTATTTTTTTGCCCCCTAAGAAATTGCCTTATGTATGATGAATTACGATTGTCCCAGTGAATGGAATTTTCCTTTTGTTTATTTATGAACGGGAATAAGGCATACCCGTGAATGGAATTGGAATCCAGCGTGTTGTTTTTGTCTTTCAGACAACGTGATTGCACTGGTGATGCCAAGTAATCAATCTTTGTATTATTGCTATTATTATTATGGTGATAAACTTTTCCGACAAAAGAACTAAATTATTATTATCAAATAACCATACGAACTTAAACACAAGTATAAATTATCTGTTATAACCTACCGATTTGACATTTTCAAACGGAAACTAAATTGATTTACCGTAATGAGAAACTCTTTTCTTAACCATCTCAAAAGTTCAACTATTTGCATGCATGGAGGTTAATATTTCTTTTAAGATTTCCAATTTGTGATGATCTCACAAAAAATGTGCCATTCCAGAAGTTCATAATTTGTCACCTGTTTTATGTTTTTAGAGAAAATGAGTCTTGCAACCATTTTTTTTCTTGTAATCTTGTAATTTTGCCATTGATGCTTCACTAAAAAAATATTAAATTGGACAACTTGAAAATAGCATGATGAGTGTGAGAAAGAAATGTCTAATACTCACCATGAGAAGAAATTAAAGGAGCAGTGTCACATTATGAAAGACGTGGAAGATAAGGACCAACAAAGCCTTTGCATGTGA
Protein sequence

>MS.gene020419.t1

MSMSNATDPCASQPDDSDLNVIPMYGKCSPFNPQKTDSWDNRVLNMASKDPARMSYLSSLLAQKTVSSAPIASGQAFNIGNYIVRVKIGTPGQLLFMVLDTSTDEAFVPSSGCTGCSATTFLPNASTSYVPLECSVPQCSQVRGLSCPATGSGACSFNKSYAGSTYSATLVQDSLRLATDVIPSYSFGSINAISGSSIPAQGLLGLGRGPLSLLSQTGSLYSGVFSYCLPSFKSYYFSGSLKLGPVGQPKSIRTTPLLRNPRRPSLYFVNLTGITVGKVNVPFPKELLAFDVNTGSGTIIDSGTVITRFVEPVYNAIRDEFRKQVTGPFSSLGAFDTCFVKNYETLAPTITLHFTDLDLKLPLENSLIHSSSGSLACLAMASAPSNHDECEKEMSNTHHEKKLKEQCHIMKDVEDKDQQSLCM