AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene016635

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016635.t1 MTR_8g038250 87.472 439 31 2 1 438 1 416 0.0 764
MS.gene016635.t1 MTR_8g096990 77.397 438 93 2 1 438 1 432 0.0 697
MS.gene016635.t1 MTR_5g080890 73.243 441 108 6 1 438 1 434 0.0 643
MS.gene016635.t1 MTR_5g080960 73.016 441 109 6 1 438 1 434 0.0 642
MS.gene016635.t1 MTR_8g038280 71.041 442 118 3 1 438 1 436 0.0 640
MS.gene016635.t1 MTR_5g044770 69.406 438 127 3 1 438 1 431 0.0 611
MS.gene016635.t1 MTR_6g090290 64.706 442 145 4 1 437 1 436 0.0 555
MS.gene016635.t1 MTR_8g038310 60.502 438 147 5 1 437 1 413 0.0 524
MS.gene016635.t1 MTR_4g109760 64.920 439 113 7 1 438 1 399 0.0 513
MS.gene016635.t1 MTR_3g028120 53.095 420 187 6 25 438 24 439 7.44e-149 431
MS.gene016635.t1 MTR_5g038360 51.025 439 199 8 10 437 3 436 1.62e-142 414
MS.gene016635.t1 MTR_2g067830 52.913 412 182 7 32 437 1 406 4.40e-139 404
MS.gene016635.t1 MTR_6g088760 53.117 401 171 9 6 395 7 401 5.21e-136 400
MS.gene016635.t1 MTR_5g044570 48.416 442 181 11 10 437 3 411 5.19e-129 379
MS.gene016635.t1 MTR_0024s0040 49.640 417 194 8 30 437 1 410 2.42e-125 369
MS.gene016635.t1 MTR_2g105300 49.633 409 172 8 32 434 1 381 2.50e-124 366
MS.gene016635.t1 MTR_0036s0050 44.144 444 219 11 9 437 6 435 9.45e-115 343
MS.gene016635.t1 MTR_0036s0070 41.913 439 227 11 9 437 6 426 3.43e-109 329
MS.gene016635.t1 MTR_0036s0110 41.518 448 233 12 6 437 8 442 3.96e-103 314
MS.gene016635.t1 MTR_5g008070 43.128 422 215 10 21 436 23 425 7.47e-102 310
MS.gene016635.t1 MTR_2g059460 40.898 423 203 11 23 438 25 407 2.02e-94 290
MS.gene016635.t1 MTR_2g059490 42.298 383 209 8 61 438 36 411 4.43e-93 288
MS.gene016635.t1 MTR_5g008110 38.739 444 219 12 7 437 12 415 8.84e-92 284
MS.gene016635.t1 MTR_5g008150 40.047 422 211 11 22 437 27 412 1.58e-91 283
MS.gene016635.t1 MTR_3g097150 36.471 425 240 14 27 438 42 449 1.01e-75 243
MS.gene016635.t1 MTR_1g060750 37.778 405 215 12 40 437 2 376 3.08e-75 240
MS.gene016635.t1 MTR_4g045857 42.188 256 141 4 61 312 36 288 1.13e-60 199
MS.gene016635.t1 MTR_5g008090 37.748 302 174 8 142 437 21 314 6.47e-59 196
MS.gene016635.t1 MTR_7g117430 35.561 419 219 18 33 435 101 484 6.77e-56 192
MS.gene016635.t1 MTR_1g115210 35.635 362 202 14 85 435 145 486 6.09e-54 187
MS.gene016635.t1 MTR_1g039270 34.341 364 205 16 85 435 127 469 2.71e-50 177
MS.gene016635.t1 MTR_6g085010 34.930 355 207 13 86 435 156 491 1.26e-47 170
MS.gene016635.t1 MTR_3g046300 31.266 387 246 9 68 438 79 461 7.55e-47 167
MS.gene016635.t1 MTR_1g111350 33.983 359 208 13 85 435 130 467 5.12e-46 166
MS.gene016635.t1 MTR_6g088765 40.756 238 107 5 163 399 5 209 1.66e-44 155
MS.gene016635.t1 MTR_5g091130 31.701 388 240 11 68 436 173 554 2.79e-43 160
MS.gene016635.t1 MTR_2g028190 32.338 402 243 14 49 435 64 451 3.97e-41 152
MS.gene016635.t1 MTR_3g053710 32.231 363 222 11 85 435 116 466 3.69e-40 149
MS.gene016635.t1 MTR_4g007740 31.421 366 215 13 87 436 133 478 4.47e-39 147
MS.gene016635.t1 MTR_8g103880 28.316 392 251 14 53 436 87 456 1.90e-36 139
MS.gene016635.t1 MTR_2g070430 28.475 446 270 16 28 437 25 457 5.24e-36 138
MS.gene016635.t1 MTR_2g055840 29.581 382 232 12 78 435 5 373 2.52e-35 134
MS.gene016635.t1 MTR_7g080230 27.703 444 261 17 30 436 11 431 3.68e-35 136
MS.gene016635.t1 MTR_1g078240 28.606 409 243 15 58 436 41 430 1.64e-34 134
MS.gene016635.t1 MTR_1g078240 29.024 410 241 16 58 436 41 431 4.61e-34 133
MS.gene016635.t1 MTR_3g068000 29.243 383 218 15 89 435 76 441 6.36e-32 127
MS.gene016635.t1 MTR_7g105850 28.631 482 270 21 9 437 7 467 3.70e-31 125
MS.gene016635.t1 MTR_3g065160 26.613 372 234 12 88 438 76 429 1.22e-30 122
MS.gene016635.t1 MTR_7g021510 29.381 388 221 19 82 436 67 434 1.66e-30 123
MS.gene016635.t1 MTR_4g008110 55.172 116 29 1 159 274 45 137 4.10e-30 114
MS.gene016635.t1 MTR_3g053980 31.680 363 218 13 85 435 141 485 3.22e-28 116
MS.gene016635.t1 MTR_8g094590 26.952 397 245 14 65 438 53 427 2.10e-27 114
MS.gene016635.t1 MTR_4g095270 25.120 418 265 17 41 438 37 426 6.93e-27 113
MS.gene016635.t1 MTR_4g095270 25.120 418 265 17 41 438 37 426 8.63e-27 113
MS.gene016635.t1 MTR_5g016260 25.061 411 250 16 49 438 61 434 1.76e-26 112
MS.gene016635.t1 MTR_1g078240 28.863 343 198 13 58 373 41 364 2.11e-26 110
MS.gene016635.t1 MTR_5g016260 25.310 403 243 16 57 438 69 434 2.19e-26 112
MS.gene016635.t1 MTR_4g056420 29.737 380 221 16 85 436 76 437 2.58e-26 111
MS.gene016635.t1 MTR_3g096930 25.258 388 243 13 78 438 70 437 4.98e-26 110
MS.gene016635.t1 MTR_5g077850 26.247 381 228 15 88 438 83 440 2.35e-25 108
MS.gene016635.t1 MTR_1g029810 26.064 376 237 14 85 438 56 412 3.00e-25 107
MS.gene016635.t1 MTR_2g063110 26.055 403 235 16 85 435 117 508 3.22e-25 108
MS.gene016635.t1 MTR_1g029810 26.064 376 237 14 85 438 56 412 3.61e-25 107
MS.gene016635.t1 MTR_7g080230 28.045 353 209 15 110 436 28 361 3.98e-25 107
MS.gene016635.t1 MTR_7g062360 27.559 381 225 16 89 435 62 425 4.37e-25 107
MS.gene016635.t1 MTR_4g023630 26.190 420 259 15 50 438 23 422 6.09e-25 106
MS.gene016635.t1 MTR_4g023650 26.415 424 253 18 50 438 23 422 8.04e-25 106
MS.gene016635.t1 MTR_1g037630 28.537 410 263 16 25 423 26 416 9.20e-25 106
MS.gene016635.t1 MTR_6g084710 27.273 385 231 16 80 435 186 550 2.67e-24 105
MS.gene016635.t1 MTR_1g061940 29.504 383 222 18 84 436 81 445 4.29e-24 104
MS.gene016635.t1 MTR_8g094590 31.047 277 164 10 65 334 53 309 7.98e-24 101
MS.gene016635.t1 MTR_8g039540 28.505 428 270 17 14 423 12 421 1.25e-23 102
MS.gene016635.t1 MTR_2g084550 30.000 380 221 17 85 436 76 438 2.05e-23 102
MS.gene016635.t1 MTR_3g014290 30.994 342 213 13 87 423 81 404 4.47e-23 101
MS.gene016635.t1 MTR_8g094590 30.070 286 173 10 65 343 53 318 5.89e-23 100
MS.gene016635.t1 MTR_7g102750 28.424 387 217 20 85 436 80 441 1.48e-22 100
MS.gene016635.t1 MTR_0945s0010 38.760 129 74 4 311 438 1 125 1.71e-22 94.0
MS.gene016635.t1 MTR_2g103340 39.416 137 39 1 20 156 33 125 2.41e-22 94.4
MS.gene016635.t1 MTR_2g103340 46.341 82 34 3 351 432 88 159 8.99e-12 64.3
MS.gene016635.t1 MTR_6g084710 29.630 297 178 14 80 365 186 462 1.06e-21 97.8
MS.gene016635.t1 MTR_4g100990 27.163 416 224 19 86 438 74 473 9.97e-21 94.7
MS.gene016635.t1 MTR_5g061690 29.577 355 197 20 93 423 108 433 8.18e-20 92.0
MS.gene016635.t1 MTR_7g102750 29.231 325 180 16 85 379 80 384 4.09e-19 89.7
MS.gene016635.t1 MTR_8g075090 27.895 380 214 20 87 437 113 461 1.99e-18 87.8
MS.gene016635.t1 MTR_8g075090 28.042 378 212 20 87 435 113 459 4.01e-18 86.7
MS.gene016635.t1 MTR_5g077920 26.738 374 235 13 88 437 70 428 1.40e-17 85.1
MS.gene016635.t1 MTR_8g075090 30.164 305 169 15 92 374 118 400 2.72e-17 84.3
MS.gene016635.t1 MTR_3g006820 24.000 400 263 15 64 438 32 415 6.16e-17 82.8
MS.gene016635.t1 MTR_5g080330 25.344 363 199 17 103 435 2 322 2.43e-15 78.2
MS.gene016635.t1 MTR_8g075010 26.247 381 217 19 87 435 101 449 1.36e-14 76.3
MS.gene016635.t1 MTR_2g037710 27.397 219 126 7 228 438 15 208 3.50e-14 71.6
MS.gene016635.t1 MTR_3g100500 28.261 414 249 21 30 422 38 424 5.24e-12 67.8
MS.gene016635.t1 MTR_3g087780 26.048 334 194 17 128 438 8 311 1.33e-11 66.2
MS.gene016635.t1 MTR_3g087780 26.048 334 194 17 128 438 44 347 2.01e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016635.t1 AT1G64830 51.208 414 195 3 26 438 24 431 7.95e-141 410
MS.gene016635.t1 AT5G33340 52.657 414 188 4 27 438 30 437 4.69e-138 403
MS.gene016635.t1 AT2G35615 45.270 444 216 8 14 436 7 444 2.41e-116 348
MS.gene016635.t1 AT1G31450 43.049 446 229 8 4 436 9 442 9.61e-108 326
MS.gene016635.t1 AT2G28010 40.639 438 207 12 5 438 6 394 9.53e-93 286
MS.gene016635.t1 AT2G28040 41.176 425 199 12 16 438 18 393 3.76e-90 279
MS.gene016635.t1 AT2G28040 43.041 388 192 10 54 438 23 384 2.10e-89 277
MS.gene016635.t1 AT2G28030 43.207 368 179 10 75 438 49 390 4.35e-85 266
MS.gene016635.t1 AT2G28220 37.868 441 221 11 5 438 6 400 5.96e-84 263
MS.gene016635.t1 AT2G28225 40.431 371 189 8 75 438 55 400 1.27e-78 249
MS.gene016635.t1 AT2G03200 36.384 437 238 14 25 438 42 461 1.88e-70 230
MS.gene016635.t1 AT1G01300 38.292 363 196 14 85 436 140 485 5.52e-56 192
MS.gene016635.t1 AT3G61820 36.869 396 215 15 55 435 108 483 8.65e-55 189
MS.gene016635.t1 AT3G18490 32.366 448 269 16 1 435 74 500 1.84e-53 186
MS.gene016635.t1 AT4G30030 36.533 375 209 15 72 435 63 419 9.65e-53 182
MS.gene016635.t1 AT2G23945 28.947 456 288 14 10 438 5 451 1.87e-47 169
MS.gene016635.t1 AT3G25700 31.019 432 244 17 39 436 39 450 1.13e-46 167
MS.gene016635.t1 AT1G25510 33.973 365 200 15 85 435 146 483 1.48e-46 167
MS.gene016635.t1 AT5G36260 32.190 379 210 15 85 436 76 434 1.76e-46 167
MS.gene016635.t1 AT5G10760 32.401 429 252 14 23 435 58 464 2.68e-46 166
MS.gene016635.t1 AT3G20015 30.474 443 242 18 28 435 59 470 1.49e-44 162
MS.gene016635.t1 AT5G10770 32.407 432 250 16 29 436 61 474 2.35e-42 156
MS.gene016635.t1 AT3G02740 30.933 375 219 14 85 436 83 440 8.20e-41 152
MS.gene016635.t1 AT3G02740 30.000 380 226 13 80 436 3 365 9.86e-41 150
MS.gene016635.t1 AT2G42980 32.737 391 231 14 72 438 145 527 2.93e-40 151
MS.gene016635.t1 AT3G59080 31.795 390 234 15 72 438 155 535 7.54e-40 150
MS.gene016635.t1 AT4G30040 30.310 452 251 21 9 430 10 427 1.52e-39 147
MS.gene016635.t1 AT1G65240 30.244 410 237 16 55 436 39 427 2.11e-38 145
MS.gene016635.t1 AT3G12700 27.983 461 278 19 5 436 26 461 5.87e-38 144
MS.gene016635.t1 AT1G79720 32.603 365 213 15 87 435 135 482 5.17e-35 135
MS.gene016635.t1 AT2G39710 29.787 376 213 15 89 430 67 425 2.21e-33 130
MS.gene016635.t1 AT5G37540 28.000 375 226 14 88 435 81 438 6.54e-32 126
MS.gene016635.t1 AT3G59080 30.052 386 210 14 72 438 155 499 1.42e-31 126
MS.gene016635.t1 AT1G66180 25.995 377 233 14 88 438 73 429 7.18e-30 120
MS.gene016635.t1 AT4G33490 24.757 412 251 13 61 438 28 414 1.71e-29 119
MS.gene016635.t1 AT3G54400 28.608 388 248 13 61 436 55 425 2.14e-29 119
MS.gene016635.t1 AT5G02190 27.394 376 222 15 96 435 82 442 3.12e-29 119
MS.gene016635.t1 AT5G43100 27.431 401 236 19 63 438 50 420 1.08e-28 118
MS.gene016635.t1 AT5G43100 27.930 401 234 20 63 438 50 420 1.65e-28 118
MS.gene016635.t1 AT3G50050 26.282 468 261 22 13 438 14 439 3.00e-27 115
MS.gene016635.t1 AT1G05840 28.000 375 234 15 85 436 78 439 4.52e-27 113
MS.gene016635.t1 AT1G44130 25.815 399 248 14 68 438 24 402 2.14e-26 110
MS.gene016635.t1 AT4G12920 27.532 385 240 14 61 435 32 387 2.58e-26 110
MS.gene016635.t1 AT1G09750 27.857 420 254 19 43 435 51 448 5.47e-26 110
MS.gene016635.t1 AT5G45120 28.851 409 218 18 87 435 83 478 1.82e-25 108
MS.gene016635.t1 AT5G22850 27.778 432 256 19 45 436 26 441 6.45e-24 104
MS.gene016635.t1 AT4G33490 28.197 305 178 11 61 349 25 304 7.59e-24 103
MS.gene016635.t1 AT2G36670 27.512 418 244 19 59 436 60 458 2.12e-23 102
MS.gene016635.t1 AT1G77480 26.110 383 229 17 85 438 65 422 5.56e-23 100
MS.gene016635.t1 AT2G36670 27.733 375 230 15 87 436 34 392 6.97e-23 100
MS.gene016635.t1 AT2G36670 27.733 375 230 15 87 436 34 392 6.97e-23 100
MS.gene016635.t1 AT1G77480 26.110 383 229 17 85 438 65 422 9.35e-23 100
MS.gene016635.t1 AT2G36670 27.851 377 227 16 87 436 105 463 1.23e-22 100
MS.gene016635.t1 AT5G07030 27.646 463 285 18 2 436 15 455 2.17e-22 99.4
MS.gene016635.t1 AT2G17760 27.059 425 246 20 45 435 55 449 1.22e-21 97.8
MS.gene016635.t1 AT3G52500 28.715 397 224 20 84 436 87 468 5.93e-21 95.5
MS.gene016635.t1 AT3G51350 25.561 446 272 18 27 435 30 452 8.65e-21 95.1
MS.gene016635.t1 AT1G08210 27.586 377 231 15 85 436 130 489 2.39e-20 94.0
MS.gene016635.t1 AT1G08210 27.586 377 231 15 85 436 116 475 2.55e-20 94.0
MS.gene016635.t1 AT1G08210 27.586 377 231 15 85 436 82 441 3.00e-20 93.6
MS.gene016635.t1 AT1G49050 25.385 390 243 15 80 437 197 570 3.52e-20 93.6
MS.gene016635.t1 AT1G49050 22.802 364 234 12 105 437 50 397 3.94e-20 92.4
MS.gene016635.t1 AT3G42550 26.923 364 201 16 87 436 78 390 2.05e-17 84.3
MS.gene016635.t1 AT1G49050 27.599 279 169 12 80 341 197 459 3.51e-17 84.0
MS.gene016635.t1 AT3G25700 27.473 273 155 10 39 293 39 286 9.30e-17 81.6
MS.gene016635.t1 AT1G08210 26.303 403 230 16 85 436 82 468 2.82e-16 81.3
MS.gene016635.t1 AT5G10080 25.370 473 272 27 16 437 13 455 4.51e-15 77.8
MS.gene016635.t1 AT3G51340 23.967 363 234 13 92 435 108 447 7.89e-15 77.0
MS.gene016635.t1 AT3G51360 24.784 464 274 22 14 436 7 436 2.25e-14 75.5
MS.gene016635.t1 AT3G51360 24.784 464 274 22 14 436 17 446 2.30e-14 75.5
MS.gene016635.t1 AT3G51360 26.344 372 218 18 92 436 10 352 1.25e-13 72.8
MS.gene016635.t1 AT5G24820 23.650 389 248 12 74 435 33 399 1.90e-13 72.0

Find 97 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTGGTGTTTATAATAATT+TGG 0.122254 8.2:-61657328 MS.gene016635:CDS
GGAGATGCCGCTGTGGTTTC+CGG 0.148862 8.2:-61657522 MS.gene016635:CDS
TTAAAATGTGCCGTTATTAT+AGG 0.181038 8.2:+61657207 None:intergenic
GGTTCTGACCTTTCCATCTT+TGG 0.183882 8.2:-61657090 MS.gene016635:CDS
ACGACAAGCAAACTCAATTT+TGG 0.203851 8.2:-61657543 MS.gene016635:CDS
GGTTGGTATAGTGGTGATTT+TGG 0.223826 8.2:+61658185 None:intergenic
ACCCCACCATTTAAGTTATA+TGG 0.242744 8.2:-61658014 MS.gene016635:CDS
GTCAACCCCTATAGTGAAAA+AGG 0.271435 8.2:-61657487 MS.gene016635:CDS
GCAATGCCATATAACTTAAA+TGG 0.273035 8.2:+61658008 None:intergenic
AACAAAAGAATAGAATTTAA+AGG 0.289730 8.2:-61657417 MS.gene016635:CDS
TCATTTCCTAAAACTGTGAT+AGG 0.290097 8.2:-61657717 MS.gene016635:CDS
ACTGCAACAAATAAACTAAT+AGG 0.318254 8.2:+61657162 None:intergenic
GAGATGCCGCTGTGGTTTCC+GGG 0.323952 8.2:-61657521 MS.gene016635:CDS
CTTGTCGTGTTTGACTCAAC+CGG 0.337489 8.2:+61657558 None:intergenic
AGCGTCTCTTATTACACAAT+TGG 0.348748 8.2:-61657625 MS.gene016635:CDS
GGTAACATCATAATTGATTC+AGG 0.364855 8.2:-61657372 MS.gene016635:CDS
TGAGGGCAGATTTGTAGAAA+TGG 0.376120 8.2:+61658102 None:intergenic
ATACAGGTAGTGACATTGTT+TGG 0.380232 8.2:-61657983 MS.gene016635:CDS
TATAGCAAAGGTTAAATTCT+CGG 0.388913 8.2:+61657256 None:intergenic
CCTAGCTCAGCAGAATTTGT+TGG 0.393293 8.2:-61657064 MS.gene016635:CDS
GCAGTTACTTCTGGGTTCCC+TGG 0.393771 8.2:-61657111 MS.gene016635:CDS
AGCTCCGGTGTAGTTGGCCT+TGG 0.399332 8.2:-61657657 MS.gene016635:CDS
GCCGCTGTGGTTTCCGGGGA+TGG 0.421556 8.2:-61657516 MS.gene016635:CDS
GTCAAATAGTAGAAAACTAT+AGG 0.426026 8.2:+61657462 None:intergenic
CTGAGCTAGGTTTCCAAAGA+TGG 0.426813 8.2:+61657077 None:intergenic
GTGTTTGACTCAACCGGGTT+TGG 0.428845 8.2:+61657564 None:intergenic
TTGATTGAGGTGTGTTTATG+AGG 0.430036 8.2:+61658084 None:intergenic
GGAATCAGCTGTGTTGGAAT+TGG 0.432066 8.2:-61657307 MS.gene016635:CDS
ATAGCAAAGGTTAAATTCTC+GGG 0.433218 8.2:+61657257 None:intergenic
TCCGGTGTAGTTGGCCTTGG+AGG 0.448012 8.2:-61657654 MS.gene016635:CDS
ACAAAAGAATAGAATTTAAA+GGG 0.448273 8.2:-61657416 MS.gene016635:CDS
GTTCAAACTTAGGAGTGGTT+TGG 0.448701 8.2:+61657928 None:intergenic
ATGAGATACTCACCATGATC+GGG 0.452999 8.2:+61658053 None:intergenic
TGGTGGGGTACCAACTGAAT+AGG 0.458426 8.2:+61658028 None:intergenic
GACAATACAATGTCATTTGA+AGG 0.463678 8.2:-61657684 MS.gene016635:CDS
AAAGAGAAATAATGAAACAA+AGG 0.467908 8.2:+61658258 None:intergenic
ACGTTGACAGTTATTCCAAC+TGG 0.472429 8.2:-61657345 MS.gene016635:CDS
CCTAAAACTGTGATAGGATG+TGG 0.472735 8.2:-61657711 MS.gene016635:CDS
AAGAGAAATAATGAAACAAA+GGG 0.474915 8.2:+61658259 None:intergenic
TAATTTGGAATCAGCTGTGT+TGG 0.477151 8.2:-61657313 MS.gene016635:CDS
AAATTATTATAAACACCAGT+TGG 0.480158 8.2:+61657330 None:intergenic
GGTGTAGTTGGCCTTGGAGG+TGG 0.481284 8.2:-61657651 MS.gene016635:CDS
GCGTCTCTTATTACACAATT+GGG 0.481758 8.2:-61657624 MS.gene016635:CDS
CTGATTGTAGCAAAATGTAA+TGG 0.484105 8.2:-61656999 None:intergenic
TTATATGGCATTGCTGATAC+AGG 0.486441 8.2:-61657999 MS.gene016635:CDS
GAATTTAAAGGGTCTTCAAA+TGG 0.487908 8.2:-61657405 MS.gene016635:CDS
CCAACAAATTCTGCTGAGCT+AGG 0.493770 8.2:+61657064 None:intergenic
CTTGTTGCACAGATTTACAT+AGG 0.501571 8.2:+61657862 None:intergenic
TGGTTGTAACATTCTTTGCA+AGG 0.508371 8.2:+61657948 None:intergenic
GGTGCAAGCTCCGGTGTAGT+TGG 0.509113 8.2:-61657663 MS.gene016635:CDS
TGCCATATAACTTAAATGGT+GGG 0.513388 8.2:+61658012 None:intergenic
TGCTATAGTGTCACATCAGA+TGG 0.514224 8.2:-61657240 MS.gene016635:CDS
CAAAGGTTAAATTCTCGGGT+TGG 0.519243 8.2:+61657261 None:intergenic
TTATTGTTTGTTGCCAAACC+CGG 0.519426 8.2:-61657577 MS.gene016635:CDS
TTATTTGTTGCAGTTGCAGA+TGG 0.522932 8.2:-61657153 MS.gene016635:CDS
ACCTCCAAGGCCAACTACAC+CGG 0.524986 8.2:+61657653 None:intergenic
TCATTTGAAGGTGCAAGCTC+CGG 0.527243 8.2:-61657672 MS.gene016635:CDS
TTGTCGTGTTTGACTCAACC+GGG 0.535482 8.2:+61657559 None:intergenic
CATGAGATACTCACCATGAT+CGG 0.536629 8.2:+61658052 None:intergenic
GTCTTCAAATGGTGGTGATG+AGG 0.541942 8.2:-61657394 MS.gene016635:CDS
GGTTGGCACGGTTTCTAGAA+CGG 0.543629 8.2:+61658123 None:intergenic
GGCAGATTTGTAGAAATGGT+TGG 0.544857 8.2:+61658106 None:intergenic
TCAACTGTAATCCCCGATCA+TGG 0.547516 8.2:-61658065 MS.gene016635:CDS
CTTTCTCATGCTTTAAACAA+CGG 0.553785 8.2:-61658239 MS.gene016635:CDS
ACATTGGAAGCATTTAGTGT+TGG 0.556371 8.2:-61657441 MS.gene016635:CDS
AAGATGGAAAGGTCAGAACC+AGG 0.560391 8.2:+61657093 None:intergenic
TCTTCAAATGGTGGTGATGA+GGG 0.564161 8.2:-61657393 MS.gene016635:CDS
CTTACATTAGAGTCCTCCAC+TGG 0.566167 8.2:-61657750 MS.gene016635:CDS
ATGCCATATAACTTAAATGG+TGG 0.567034 8.2:+61658011 None:intergenic
TCGGGGATTACAGTTGATTG+AGG 0.568413 8.2:+61658071 None:intergenic
GCCATATAACTTAAATGGTG+GGG 0.578953 8.2:+61658013 None:intergenic
GGTTGACACTACACCATCCC+CGG 0.582467 8.2:+61657503 None:intergenic
AGAAATAATGAAACAAAGGG+AGG 0.584756 8.2:+61658262 None:intergenic
GCTCAGCAGAATTTGTTGGT+TGG 0.588696 8.2:-61657060 MS.gene016635:CDS
AACAAAGGGAGGAGTAAAAG+AGG 0.588818 8.2:+61658273 None:intergenic
GAGACGCTGGTCCACCTCCA+AGG 0.588914 8.2:+61657640 None:intergenic
TTTAAAGGGTCTTCAAATGG+TGG 0.588943 8.2:-61657402 MS.gene016635:CDS
ATTTGTAGAAATGGTTGGCA+CGG 0.592947 8.2:+61658111 None:intergenic
CTAGGTTTCCAAAGATGGAA+AGG 0.601783 8.2:+61657082 None:intergenic
TGATGGTTCAAACTTAGGAG+TGG 0.603375 8.2:+61657923 None:intergenic
TGAAATAGGTCGCCCAGTGG+AGG 0.608785 8.2:+61657737 None:intergenic
GGTGATACGTCACAATCGAA+AGG 0.619221 8.2:-61657792 MS.gene016635:CDS
CTGATGTGACACTATAGCAA+AGG 0.629182 8.2:+61657244 None:intergenic
AGATGCCGCTGTGGTTTCCG+GGG 0.633627 8.2:-61657520 MS.gene016635:CDS
TTACATAGGTCAGACGAACA+TGG 0.633649 8.2:+61657876 None:intergenic
TATCTCATGACCTATTCAGT+TGG 0.633970 8.2:-61658038 MS.gene016635:CDS
TGATTGAGGTGTGTTTATGA+GGG 0.635382 8.2:+61658085 None:intergenic
GTGCGGCATTGACAATATGT+TGG 0.644132 8.2:+61658147 None:intergenic
AGATGGAAAGGTCAGAACCA+GGG 0.650286 8.2:+61657094 None:intergenic
TTACATTAGAGTCCTCCACT+GGG 0.659506 8.2:-61657749 MS.gene016635:CDS
ACGGTTTCTAGAACGGTGTG+CGG 0.667331 8.2:+61658130 None:intergenic
TGAGATACTCACCATGATCG+GGG 0.668047 8.2:+61658054 None:intergenic
AATTGTGTAATAAGAGACGC+TGG 0.670336 8.2:+61657627 None:intergenic
CTATGTAAATCTGTGCAACA+AGG 0.670936 8.2:-61657861 MS.gene016635:CDS
GGAGTAAAAGAGGCTCAACA+AGG 0.674597 8.2:+61658283 None:intergenic
ACCATCCCCGGAAACCACAG+CGG 0.698241 8.2:+61657515 None:intergenic
AAATGAAATAGGTCGCCCAG+TGG 0.727383 8.2:+61657734 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAAAGAATAGAATTTAA+AGG - chr8.2:61657881-61657900 MS.gene016635:CDS 15.0%
!! ACAAAAGAATAGAATTTAAA+GGG - chr8.2:61657882-61657901 MS.gene016635:CDS 15.0%
!!! AAATTTTTGATCATTACAAT+CGG + chr8.2:61657465-61657484 None:intergenic 15.0%
!!! ATATGATTTTCCTATAATAA+CGG - chr8.2:61658081-61658100 MS.gene016635:CDS 15.0%
!!! TGTGAATATAGTATTTTTTA+TGG - chr8.2:61657485-61657504 MS.gene016635:CDS 15.0%
!! AAAGAGAAATAATGAAACAA+AGG + chr8.2:61657043-61657062 None:intergenic 20.0%
!! AAATTATTATAAACACCAGT+TGG + chr8.2:61657971-61657990 None:intergenic 20.0%
!! AACTGGTGTTTATAATAATT+TGG - chr8.2:61657970-61657989 MS.gene016635:CDS 20.0%
!! AAGAGAAATAATGAAACAAA+GGG + chr8.2:61657042-61657061 None:intergenic 20.0%
! ACTGCAACAAATAAACTAAT+AGG + chr8.2:61658139-61658158 None:intergenic 25.0%
! GTCAAATAGTAGAAAACTAT+AGG + chr8.2:61657839-61657858 None:intergenic 25.0%
! TATAGCAAAGGTTAAATTCT+CGG + chr8.2:61658045-61658064 None:intergenic 25.0%
!! AGTTTTCTACTATTTGACAT+TGG - chr8.2:61657841-61657860 MS.gene016635:CDS 25.0%
!! ATAATAACGGCACATTTTAA+AGG - chr8.2:61658094-61658113 MS.gene016635:CDS 25.0%
!! TTAAAATGTGCCGTTATTAT+AGG + chr8.2:61658094-61658113 None:intergenic 25.0%
!!! ACAGTTTTAGGAAATGAAAT+AGG + chr8.2:61657578-61657597 None:intergenic 25.0%
AGAAATAATGAAACAAAGGG+AGG + chr8.2:61657039-61657058 None:intergenic 30.0%
ATAGCAAAGGTTAAATTCTC+GGG + chr8.2:61658044-61658063 None:intergenic 30.0%
ATGCCATATAACTTAAATGG+TGG + chr8.2:61657290-61657309 None:intergenic 30.0%
GAATTTAAAGGGTCTTCAAA+TGG - chr8.2:61657893-61657912 MS.gene016635:CDS 30.0%
GCAATGCCATATAACTTAAA+TGG + chr8.2:61657293-61657312 None:intergenic 30.0%
TCATTTCCTAAAACTGTGAT+AGG - chr8.2:61657581-61657600 MS.gene016635:CDS 30.0%
TGCCATATAACTTAAATGGT+GGG + chr8.2:61657289-61657308 None:intergenic 30.0%
! CTTTCTCATGCTTTAAACAA+CGG - chr8.2:61657059-61657078 MS.gene016635:CDS 30.0%
! GACAATACAATGTCATTTGA+AGG - chr8.2:61657614-61657633 MS.gene016635:CDS 30.0%
! GGTAACATCATAATTGATTC+AGG - chr8.2:61657926-61657945 MS.gene016635:CDS 30.0%
! TACATTTTGCTACAATCAGT+AGG + chr8.2:61658298-61658317 None:intergenic 30.0%
!! ATAGGATCCTTTTTCACTAT+AGG + chr8.2:61657821-61657840 None:intergenic 30.0%
!! GATTTTGATGGTTCAAACTT+AGG + chr8.2:61657383-61657402 None:intergenic 30.0%
!! TAGGATCCTTTTTCACTATA+GGG + chr8.2:61657820-61657839 None:intergenic 30.0%
!! TTGTAAGTTGACGATTTTGA+TGG + chr8.2:61657395-61657414 None:intergenic 30.0%
!!! TGTTGGTATTTGTTTTGTGT+AGG + chr8.2:61657137-61657156 None:intergenic 30.0%
AATTGTGTAATAAGAGACGC+TGG + chr8.2:61657674-61657693 None:intergenic 35.0%
AGCGTCTCTTATTACACAAT+TGG - chr8.2:61657673-61657692 MS.gene016635:CDS 35.0%
ATACAGGTAGTGACATTGTT+TGG - chr8.2:61657315-61657334 MS.gene016635:CDS 35.0%
ATTTGTAGAAATGGTTGGCA+CGG + chr8.2:61657190-61657209 None:intergenic 35.0%
CTATGTAAATCTGTGCAACA+AGG - chr8.2:61657437-61657456 MS.gene016635:CDS 35.0%
CTTGTTGCACAGATTTACAT+AGG + chr8.2:61657439-61657458 None:intergenic 35.0%
GCCATATAACTTAAATGGTG+GGG + chr8.2:61657288-61657307 None:intergenic 35.0%
GCGTCTCTTATTACACAATT+GGG - chr8.2:61657674-61657693 MS.gene016635:CDS 35.0%
TATCTCATGACCTATTCAGT+TGG - chr8.2:61657260-61657279 MS.gene016635:CDS 35.0%
TGATTGAGGTGTGTTTATGA+GGG + chr8.2:61657216-61657235 None:intergenic 35.0%
TGGTTGTAACATTCTTTGCA+AGG + chr8.2:61657353-61657372 None:intergenic 35.0%
TTATATGGCATTGCTGATAC+AGG - chr8.2:61657299-61657318 MS.gene016635:CDS 35.0%
TTGATTGAGGTGTGTTTATG+AGG + chr8.2:61657217-61657236 None:intergenic 35.0%
TTTAAAGGGTCTTCAAATGG+TGG - chr8.2:61657896-61657915 MS.gene016635:CDS 35.0%
! ACCCCACCATTTAAGTTATA+TGG - chr8.2:61657284-61657303 MS.gene016635:CDS 35.0%
! ACGACAAGCAAACTCAATTT+TGG - chr8.2:61657755-61657774 MS.gene016635:CDS 35.0%
! ACGACACAATTTTTCGTTGA+AGG + chr8.2:61658270-61658289 None:intergenic 35.0%
! GTTGGTATAGTGGTGATTTT+GGG + chr8.2:61657115-61657134 None:intergenic 35.0%
! TAATTTGGAATCAGCTGTGT+TGG - chr8.2:61657985-61658004 MS.gene016635:CDS 35.0%
! TTATTGTTTGTTGCCAAACC+CGG - chr8.2:61657721-61657740 MS.gene016635:CDS 35.0%
! TTATTTGTTGCAGTTGCAGA+TGG - chr8.2:61658145-61658164 MS.gene016635:CDS 35.0%
! TTGGTATAGTGGTGATTTTG+GGG + chr8.2:61657114-61657133 None:intergenic 35.0%
!! ACATTGGAAGCATTTAGTGT+TGG - chr8.2:61657857-61657876 MS.gene016635:CDS 35.0%
!! AGGATCCTTTTTCACTATAG+GGG + chr8.2:61657819-61657838 None:intergenic 35.0%
!! TTGCTTTTGCAGTTACTTCT+GGG - chr8.2:61658179-61658198 MS.gene016635:CDS 35.0%
!! TTTGCTTTTGCAGTTACTTC+TGG - chr8.2:61658178-61658197 MS.gene016635:CDS 35.0%
!!! GGTATTTGTTTTGTGTAGGT+TGG + chr8.2:61657133-61657152 None:intergenic 35.0%
!!! TTTTGTGTAGGTTGGTATAG+TGG + chr8.2:61657125-61657144 None:intergenic 35.0%
AACAAAGGGAGGAGTAAAAG+AGG + chr8.2:61657028-61657047 None:intergenic 40.0%
ACGTTGACAGTTATTCCAAC+TGG - chr8.2:61657953-61657972 MS.gene016635:CDS 40.0%
ATGAGATACTCACCATGATC+GGG + chr8.2:61657248-61657267 None:intergenic 40.0%
CAAAGGTTAAATTCTCGGGT+TGG + chr8.2:61658040-61658059 None:intergenic 40.0%
CATGAGATACTCACCATGAT+CGG + chr8.2:61657249-61657268 None:intergenic 40.0%
CCTAAAACTGTGATAGGATG+TGG - chr8.2:61657587-61657606 MS.gene016635:CDS 40.0%
CTAGGTTTCCAAAGATGGAA+AGG + chr8.2:61658219-61658238 None:intergenic 40.0%
CTGATGTGACACTATAGCAA+AGG + chr8.2:61658057-61658076 None:intergenic 40.0%
GGCAGATTTGTAGAAATGGT+TGG + chr8.2:61657195-61657214 None:intergenic 40.0%
GTCAACCCCTATAGTGAAAA+AGG - chr8.2:61657811-61657830 MS.gene016635:CDS 40.0%
GTTCAAACTTAGGAGTGGTT+TGG + chr8.2:61657373-61657392 None:intergenic 40.0%
TGAGGGCAGATTTGTAGAAA+TGG + chr8.2:61657199-61657218 None:intergenic 40.0%
TGATGGTTCAAACTTAGGAG+TGG + chr8.2:61657378-61657397 None:intergenic 40.0%
TGCTATAGTGTCACATCAGA+TGG - chr8.2:61658058-61658077 MS.gene016635:CDS 40.0%
TTACATAGGTCAGACGAACA+TGG + chr8.2:61657425-61657444 None:intergenic 40.0%
TTACATTAGAGTCCTCCACT+GGG - chr8.2:61657549-61657568 MS.gene016635:CDS 40.0%
! CCACATCCTATCACAGTTTT+AGG + chr8.2:61657590-61657609 None:intergenic 40.0%
! TCTTCAAATGGTGGTGATGA+GGG - chr8.2:61657905-61657924 MS.gene016635:CDS 40.0%
!! GGTTGGTATAGTGGTGATTT+TGG + chr8.2:61657116-61657135 None:intergenic 40.0%
AAATGAAATAGGTCGCCCAG+TGG + chr8.2:61657567-61657586 None:intergenic 45.0%
AAGATGGAAAGGTCAGAACC+AGG + chr8.2:61658208-61658227 None:intergenic 45.0%
AGATGGAAAGGTCAGAACCA+GGG + chr8.2:61658207-61658226 None:intergenic 45.0%
CCAACAAATTCTGCTGAGCT+AGG + chr8.2:61658237-61658256 None:intergenic 45.0%
CCTAGCTCAGCAGAATTTGT+TGG - chr8.2:61658234-61658253 MS.gene016635:CDS 45.0%
CTGAGCTAGGTTTCCAAAGA+TGG + chr8.2:61658224-61658243 None:intergenic 45.0%
CTTACATTAGAGTCCTCCAC+TGG - chr8.2:61657548-61657567 MS.gene016635:CDS 45.0%
CTTGTCGTGTTTGACTCAAC+CGG + chr8.2:61657743-61657762 None:intergenic 45.0%
GGAATCAGCTGTGTTGGAAT+TGG - chr8.2:61657991-61658010 MS.gene016635:CDS 45.0%
GGAGTAAAAGAGGCTCAACA+AGG + chr8.2:61657018-61657037 None:intergenic 45.0%
GGTGATACGTCACAATCGAA+AGG - chr8.2:61657506-61657525 MS.gene016635:CDS 45.0%
TCAACTGTAATCCCCGATCA+TGG - chr8.2:61657233-61657252 MS.gene016635:CDS 45.0%
TCGGGGATTACAGTTGATTG+AGG + chr8.2:61657230-61657249 None:intergenic 45.0%
TGAGATACTCACCATGATCG+GGG + chr8.2:61657247-61657266 None:intergenic 45.0%
TTGTCGTGTTTGACTCAACC+GGG + chr8.2:61657742-61657761 None:intergenic 45.0%
! GGTTCTGACCTTTCCATCTT+TGG - chr8.2:61658208-61658227 MS.gene016635:CDS 45.0%
! GTCTTCAAATGGTGGTGATG+AGG - chr8.2:61657904-61657923 MS.gene016635:CDS 45.0%
! TCATTTGAAGGTGCAAGCTC+CGG - chr8.2:61657626-61657645 MS.gene016635:CDS 45.0%
!! GCTCAGCAGAATTTGTTGGT+TGG - chr8.2:61658238-61658257 MS.gene016635:CDS 45.0%
!! GTGCGGCATTGACAATATGT+TGG + chr8.2:61657154-61657173 None:intergenic 45.0%
GTGTTTGACTCAACCGGGTT+TGG + chr8.2:61657737-61657756 None:intergenic 50.0%
! GGTTGGCACGGTTTCTAGAA+CGG + chr8.2:61657178-61657197 None:intergenic 50.0%
! TGGTGGGGTACCAACTGAAT+AGG + chr8.2:61657273-61657292 None:intergenic 50.0%
!! ACGGTTTCTAGAACGGTGTG+CGG + chr8.2:61657171-61657190 None:intergenic 50.0%
!! CAATTTTGGAGATGCCGCTG+TGG - chr8.2:61657769-61657788 MS.gene016635:CDS 50.0%
ACCTCCAAGGCCAACTACAC+CGG + chr8.2:61657648-61657667 None:intergenic 55.0%
GCAGTTACTTCTGGGTTCCC+TGG - chr8.2:61658187-61658206 MS.gene016635:CDS 55.0%
GGTTGACACTACACCATCCC+CGG + chr8.2:61657798-61657817 None:intergenic 55.0%
TGAAATAGGTCGCCCAGTGG+AGG + chr8.2:61657564-61657583 None:intergenic 55.0%
!! GTGGTGATTTTGGGGAGTCG+CGG + chr8.2:61657106-61657125 None:intergenic 55.0%
ACCATCCCCGGAAACCACAG+CGG + chr8.2:61657786-61657805 None:intergenic 60.0%
AGATGCCGCTGTGGTTTCCG+GGG - chr8.2:61657778-61657797 MS.gene016635:CDS 60.0%
GAGATGCCGCTGTGGTTTCC+GGG - chr8.2:61657777-61657796 MS.gene016635:CDS 60.0%
GGAGATGCCGCTGTGGTTTC+CGG - chr8.2:61657776-61657795 MS.gene016635:CDS 60.0%
GGTGCAAGCTCCGGTGTAGT+TGG - chr8.2:61657635-61657654 MS.gene016635:CDS 60.0%
! AGCTCCGGTGTAGTTGGCCT+TGG - chr8.2:61657641-61657660 MS.gene016635:CDS 60.0%
!! GGTGTAGTTGGCCTTGGAGG+TGG - chr8.2:61657647-61657666 MS.gene016635:CDS 60.0%
!! GTGATTTTGGGGAGTCGCGG+TGG + chr8.2:61657103-61657122 None:intergenic 60.0%
!! TCCGGTGTAGTTGGCCTTGG+AGG - chr8.2:61657644-61657663 MS.gene016635:CDS 60.0%
GAGACGCTGGTCCACCTCCA+AGG + chr8.2:61657661-61657680 None:intergenic 65.0%
GCCGCTGTGGTTTCCGGGGA+TGG - chr8.2:61657782-61657801 MS.gene016635:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 61657002 61658318 61657002 ID=MS.gene016635
chr8.2 mRNA 61657002 61658318 61657002 ID=MS.gene016635.t1;Parent=MS.gene016635
chr8.2 exon 61657002 61658318 61657002 ID=MS.gene016635.t1.exon1;Parent=MS.gene016635.t1
chr8.2 CDS 61657002 61658318 61657002 ID=cds.MS.gene016635.t1;Parent=MS.gene016635.t1
Gene Sequence

>MS.gene016635

ATGAATACATGTTCCTTGTTGAGCCTCTTTTACTCCTCCCTTTGTTTCATTATTTCTCTTTCTCATGCTTTAAACAACGGTTTTAGTGTTGAACTCATCCACCGCGACTCCCCAAAATCACCACTATACCAACCTACACAAAACAAATACCAACATATTGTCAATGCCGCACACCGTTCTAGAAACCGTGCCAACCATTTCTACAAATCTGCCCTCATAAACACACCTCAATCAACTGTAATCCCCGATCATGGTGAGTATCTCATGACCTATTCAGTTGGTACCCCACCATTTAAGTTATATGGCATTGCTGATACAGGTAGTGACATTGTTTGGCTTCAATGCGAGCCTTGCAAAGAATGTTACAACCAAACCACTCCTAAGTTTGAACCATCAAAATCGTCAACTTACAAAAACATTCCATGTTCGTCTGACCTATGTAAATCTGTGCAACAAGGTGCCGATTGTAATGATCAAAAATTTTGTGAATATAGTATTTTTTATGGTGATACGTCACAATCGAAAGGAAATCTTAGTGTCGATACTCTTACATTAGAGTCCTCCACTGGGCGACCTATTTCATTTCCTAAAACTGTGATAGGATGTGGAACTGACAATACAATGTCATTTGAAGGTGCAAGCTCCGGTGTAGTTGGCCTTGGAGGTGGACCAGCGTCTCTTATTACACAATTGGGATCATCAATTGATTCAAAATTCTCTTATTGTTTGTTGCCAAACCCGGTTGAGTCAAACACGACAAGCAAACTCAATTTTGGAGATGCCGCTGTGGTTTCCGGGGATGGTGTAGTGTCAACCCCTATAGTGAAAAAGGATCCTATAGTTTTCTACTATTTGACATTGGAAGCATTTAGTGTTGGTAACAAAAGAATAGAATTTAAAGGGTCTTCAAATGGTGGTGATGAGGGTAACATCATAATTGATTCAGGTACAACGTTGACAGTTATTCCAACTGGTGTTTATAATAATTTGGAATCAGCTGTGTTGGAATTGGTGAAACTGAAGCGTGTTGATGATCCAACCCGAGAATTTAACCTTTGCTATAGTGTCACATCAGATGGATATGATTTTCCTATAATAACGGCACATTTTAAAGGTGCAGATGTTAAGTTGCATCCTATTAGTTTATTTGTTGCAGTTGCAGATGGCGTTGTTTGCTTTGCTTTTGCAGTTACTTCTGGGTTCCCTGGTTCTGACCTTTCCATCTTTGGAAACCTAGCTCAGCAGAATTTGTTGGTTGGTTATGACCTTCAACGAAAAATTGTGTCGTTTAAGCCTACTGATTGTAGCAAAATGTAA
Protein sequence

>MS.gene016635.t1

MNTCSLLSLFYSSLCFIISLSHALNNGFSVELIHRDSPKSPLYQPTQNKYQHIVNAAHRSRNRANHFYKSALINTPQSTVIPDHGEYLMTYSVGTPPFKLYGIADTGSDIVWLQCEPCKECYNQTTPKFEPSKSSTYKNIPCSSDLCKSVQQGADCNDQKFCEYSIFYGDTSQSKGNLSVDTLTLESSTGRPISFPKTVIGCGTDNTMSFEGASSGVVGLGGGPASLITQLGSSIDSKFSYCLLPNPVESNTTSKLNFGDAAVVSGDGVVSTPIVKKDPIVFYYLTLEAFSVGNKRIEFKGSSNGGDEGNIIIDSGTTLTVIPTGVYNNLESAVLELVKLKRVDDPTREFNLCYSVTSDGYDFPIITAHFKGADVKLHPISLFVAVADGVVCFAFAVTSGFPGSDLSIFGNLAQQNLLVGYDLQRKIVSFKPTDCSKM