AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene013256

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene013256.t1 MTR_3g097150 97.494 439 11 0 1 439 11 449 0.0 880
MS.gene013256.t1 MTR_5g080890 38.824 425 237 12 26 439 22 434 3.42e-86 270
MS.gene013256.t1 MTR_5g044770 38.863 422 231 13 30 439 25 431 6.95e-86 269
MS.gene013256.t1 MTR_5g080960 38.353 425 239 12 26 439 22 434 3.54e-84 265
MS.gene013256.t1 MTR_8g096990 37.857 420 235 13 32 439 27 432 6.81e-83 261
MS.gene013256.t1 MTR_0036s0070 38.278 418 228 10 32 438 28 426 3.34e-80 254
MS.gene013256.t1 MTR_1g111350 39.344 427 231 11 15 436 64 467 7.03e-80 254
MS.gene013256.t1 MTR_1g115210 36.190 420 243 10 31 436 78 486 1.17e-77 249
MS.gene013256.t1 MTR_1g039270 36.744 430 244 11 25 436 50 469 1.37e-77 249
MS.gene013256.t1 MTR_0036s0110 34.490 461 253 14 5 438 4 442 8.84e-76 243
MS.gene013256.t1 MTR_8g038280 35.797 433 246 14 23 439 20 436 2.39e-75 242
MS.gene013256.t1 MTR_7g117430 38.771 423 237 12 28 437 72 485 1.19e-74 242
MS.gene013256.t1 MTR_2g059460 37.783 397 217 14 57 439 27 407 4.58e-73 235
MS.gene013256.t1 MTR_2g059490 37.795 381 214 9 77 439 36 411 3.02e-72 234
MS.gene013256.t1 MTR_5g008150 38.684 380 210 10 71 438 44 412 3.05e-71 231
MS.gene013256.t1 MTR_6g090290 34.499 429 258 9 23 438 18 436 6.53e-71 231
MS.gene013256.t1 MTR_5g008110 37.127 369 210 9 82 438 57 415 2.00e-70 229
MS.gene013256.t1 MTR_8g038250 35.446 426 221 14 32 439 27 416 2.07e-69 226
MS.gene013256.t1 MTR_0036s0050 34.507 426 242 11 32 438 28 435 2.26e-69 226
MS.gene013256.t1 MTR_5g008070 38.889 360 201 9 86 436 75 424 6.07e-69 225
MS.gene013256.t1 MTR_3g028120 35.934 423 247 11 32 439 26 439 7.70e-69 225
MS.gene013256.t1 MTR_2g067830 33.967 421 244 11 37 438 1 406 4.14e-68 222
MS.gene013256.t1 MTR_8g038310 34.375 416 234 9 32 438 28 413 2.99e-67 220
MS.gene013256.t1 MTR_5g038360 34.752 423 249 12 32 438 25 436 2.14e-66 219
MS.gene013256.t1 MTR_6g085010 40.346 347 194 7 92 436 156 491 1.72e-65 218
MS.gene013256.t1 MTR_3g046300 36.111 432 238 12 38 439 38 461 8.92e-65 215
MS.gene013256.t1 MTR_5g044570 33.333 420 233 9 32 438 26 411 1.47e-63 211
MS.gene013256.t1 MTR_5g091130 37.471 435 234 16 34 436 125 553 4.76e-63 213
MS.gene013256.t1 MTR_4g007740 37.469 403 209 17 58 436 94 477 6.06e-62 208
MS.gene013256.t1 MTR_8g103880 34.270 356 221 8 86 437 110 456 8.44e-61 204
MS.gene013256.t1 MTR_2g105300 34.821 336 198 9 111 434 54 380 8.35e-59 197
MS.gene013256.t1 MTR_0024s0040 34.826 402 240 11 48 438 20 410 9.90e-58 195
MS.gene013256.t1 MTR_4g109760 39.053 338 186 10 111 439 73 399 1.86e-57 194
MS.gene013256.t1 MTR_2g028190 34.878 410 225 17 50 436 61 451 2.80e-57 195
MS.gene013256.t1 MTR_3g053710 36.885 366 200 13 89 436 114 466 6.59e-54 187
MS.gene013256.t1 MTR_4g045857 44.737 228 118 3 78 297 44 271 1.35e-53 181
MS.gene013256.t1 MTR_1g060750 31.941 407 224 14 46 438 9 376 1.08e-52 181
MS.gene013256.t1 MTR_7g105850 34.177 395 197 17 91 438 89 467 3.22e-49 174
MS.gene013256.t1 MTR_3g053980 35.068 442 228 20 27 437 73 486 5.20e-46 166
MS.gene013256.t1 MTR_2g055840 29.737 380 235 9 81 436 2 373 1.85e-45 162
MS.gene013256.t1 MTR_3g065160 32.337 368 213 11 94 439 76 429 4.59e-45 162
MS.gene013256.t1 MTR_7g062360 31.008 387 215 14 90 439 57 428 1.65e-43 158
MS.gene013256.t1 MTR_6g088760 29.539 369 230 9 32 382 27 383 1.98e-43 159
MS.gene013256.t1 MTR_1g037630 29.426 401 242 13 44 429 47 421 1.05e-42 155
MS.gene013256.t1 MTR_8g039540 29.353 402 242 14 44 429 51 426 2.13e-42 155
MS.gene013256.t1 MTR_3g014290 29.177 401 243 13 44 429 35 409 2.62e-42 154
MS.gene013256.t1 MTR_2g070430 29.630 432 259 14 34 433 32 450 3.09e-42 155
MS.gene013256.t1 MTR_5g008090 34.395 314 183 11 138 438 11 314 1.89e-40 147
MS.gene013256.t1 MTR_3g068000 30.789 380 211 15 95 436 76 441 1.53e-39 148
MS.gene013256.t1 MTR_5g016260 30.601 366 221 14 90 439 86 434 8.61e-39 147
MS.gene013256.t1 MTR_5g016260 30.601 366 221 14 90 439 86 434 1.55e-38 147
MS.gene013256.t1 MTR_4g095270 30.978 368 217 14 90 439 78 426 2.42e-38 146
MS.gene013256.t1 MTR_2g063110 28.016 514 274 21 1 437 15 509 2.68e-38 145
MS.gene013256.t1 MTR_4g095270 30.978 368 217 14 90 439 78 426 3.47e-38 146
MS.gene013256.t1 MTR_5g077850 31.351 370 218 11 94 439 83 440 4.00e-37 141
MS.gene013256.t1 MTR_2g084550 30.807 409 231 19 60 437 51 438 3.66e-35 136
MS.gene013256.t1 MTR_4g100990 29.138 429 215 20 81 439 64 473 3.25e-34 133
MS.gene013256.t1 MTR_4g056420 29.508 427 247 22 49 437 27 437 3.53e-34 133
MS.gene013256.t1 MTR_1g061940 28.406 433 258 18 35 437 35 445 1.48e-33 132
MS.gene013256.t1 MTR_5g012490 27.725 422 227 18 82 439 65 472 2.92e-32 128
MS.gene013256.t1 MTR_3g100500 28.536 403 250 12 51 437 58 438 2.98e-32 127
MS.gene013256.t1 MTR_7g102750 29.894 378 218 19 91 437 80 441 1.03e-31 127
MS.gene013256.t1 MTR_1g078240 28.871 381 220 17 91 437 68 431 4.20e-29 119
MS.gene013256.t1 MTR_7g021510 28.421 380 221 18 91 436 71 433 8.42e-29 118
MS.gene013256.t1 MTR_7g102750 30.966 352 196 19 91 411 80 415 1.10e-28 117
MS.gene013256.t1 MTR_5g077920 29.539 369 226 10 94 438 70 428 3.67e-28 115
MS.gene013256.t1 MTR_1g078240 28.871 381 219 18 91 437 68 430 4.95e-28 116
MS.gene013256.t1 MTR_5g022880 28.184 369 220 12 93 436 113 461 1.14e-25 109
MS.gene013256.t1 MTR_8g094590 28.682 387 207 22 91 439 72 427 1.22e-24 105
MS.gene013256.t1 MTR_1g078240 30.351 313 175 15 91 377 68 363 1.67e-24 104
MS.gene013256.t1 MTR_3g096930 25.815 399 227 22 80 439 69 437 2.27e-24 105
MS.gene013256.t1 MTR_8g075010 26.796 362 226 12 97 436 105 449 1.84e-23 103
MS.gene013256.t1 MTR_7g080230 26.316 380 229 16 91 437 70 431 1.27e-22 100
MS.gene013256.t1 MTR_8g094590 29.240 342 182 20 91 401 72 384 2.91e-21 95.9
MS.gene013256.t1 MTR_8g094590 30.196 255 145 12 91 333 72 305 1.52e-20 92.0
MS.gene013256.t1 MTR_5g080330 27.762 353 200 16 109 437 2 323 5.63e-20 92.0
MS.gene013256.t1 MTR_6g084710 27.204 397 231 19 78 436 174 550 1.39e-19 91.7
MS.gene013256.t1 MTR_1g029810 26.042 384 222 19 91 439 56 412 1.78e-19 90.5
MS.gene013256.t1 MTR_1g029810 25.581 387 220 18 91 439 56 412 1.84e-19 90.5
MS.gene013256.t1 MTR_2g022430 27.481 393 208 21 93 431 40 409 3.38e-18 86.7
MS.gene013256.t1 MTR_8g075090 24.339 378 225 14 93 438 113 461 5.99e-17 83.6
MS.gene013256.t1 MTR_8g075090 23.873 377 230 14 93 439 113 462 6.39e-17 83.2
MS.gene013256.t1 MTR_8g075090 25.356 351 201 15 93 411 113 434 7.84e-17 82.8
MS.gene013256.t1 MTR_4g023650 25.515 388 227 20 90 439 59 422 8.73e-17 82.4
MS.gene013256.t1 MTR_1g072420 24.173 393 230 17 88 431 42 415 1.13e-16 82.0
MS.gene013256.t1 MTR_2g022380 26.234 385 214 20 97 431 44 408 2.38e-16 81.3
MS.gene013256.t1 MTR_5g061690 22.689 357 234 14 97 431 106 442 2.95e-16 81.3
MS.gene013256.t1 MTR_6g084710 27.152 302 179 14 78 357 174 456 3.83e-16 80.9
MS.gene013256.t1 MTR_2g022350 26.234 385 214 20 97 431 44 408 2.33e-15 78.2
MS.gene013256.t1 MTR_1g072450 25.000 392 227 19 88 431 42 414 1.07e-14 76.3
MS.gene013256.t1 MTR_2g022490 24.713 348 205 14 93 401 44 373 1.72e-14 75.5
MS.gene013256.t1 MTR_4g023630 24.806 387 231 17 90 439 59 422 2.82e-14 74.7
MS.gene013256.t1 MTR_7g080230 25.648 347 207 16 124 437 33 361 8.50e-14 73.2
MS.gene013256.t1 MTR_1g093100 25.686 401 230 16 65 430 28 395 1.35e-13 72.8
MS.gene013256.t1 MTR_2g022320 26.246 301 162 13 79 338 21 302 1.55e-13 72.4
MS.gene013256.t1 MTR_2g022480 24.355 349 205 13 93 401 50 379 1.87e-13 72.4
MS.gene013256.t1 MTR_2g022370 24.935 385 223 20 93 431 40 404 5.90e-13 70.9
MS.gene013256.t1 MTR_0945s0010 31.008 129 82 4 314 439 1 125 1.45e-11 62.8
MS.gene013256.t1 MTR_2g037650 28.571 140 89 2 45 181 68 199 1.47e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene013256.t1 AT2G03200 60.544 441 153 8 16 439 25 461 0.0 553
MS.gene013256.t1 AT1G25510 40.284 422 221 11 34 436 74 483 1.66e-78 252
MS.gene013256.t1 AT3G18490 41.848 368 196 10 76 436 144 500 2.60e-78 252
MS.gene013256.t1 AT5G33340 37.204 422 237 9 32 439 30 437 1.20e-76 245
MS.gene013256.t1 AT1G64830 37.011 435 235 12 24 439 17 431 1.93e-75 242
MS.gene013256.t1 AT3G25700 35.248 383 218 11 82 436 69 449 2.19e-74 240
MS.gene013256.t1 AT1G01300 38.333 420 232 13 35 436 74 484 8.46e-74 239
MS.gene013256.t1 AT3G61820 42.049 371 194 10 79 436 121 483 3.34e-73 238
MS.gene013256.t1 AT3G20015 34.862 436 252 12 22 436 46 470 1.27e-70 231
MS.gene013256.t1 AT2G35615 37.875 433 227 15 33 437 26 444 9.06e-70 228
MS.gene013256.t1 AT1G31450 38.272 405 219 14 57 437 45 442 2.88e-62 208
MS.gene013256.t1 AT5G10770 35.556 405 227 12 49 437 88 474 2.62e-60 204
MS.gene013256.t1 AT3G59080 37.121 396 212 14 71 436 144 532 1.69e-58 201
MS.gene013256.t1 AT2G28040 34.819 359 194 11 92 439 55 384 3.41e-56 191
MS.gene013256.t1 AT2G28040 34.819 359 194 11 92 439 64 393 3.49e-56 191
MS.gene013256.t1 AT1G79720 35.349 430 229 17 38 437 73 483 1.04e-55 192
MS.gene013256.t1 AT2G42980 37.337 383 209 15 80 436 147 524 1.07e-54 190
MS.gene013256.t1 AT2G28030 35.493 355 196 11 93 439 61 390 2.61e-54 186
MS.gene013256.t1 AT2G28010 33.333 384 222 13 64 439 37 394 6.07e-54 185
MS.gene013256.t1 AT3G52500 31.887 461 250 17 23 437 26 468 5.90e-53 184
MS.gene013256.t1 AT2G28225 35.376 359 195 14 93 439 67 400 5.66e-52 180
MS.gene013256.t1 AT5G10760 34.515 423 243 17 29 436 61 464 7.49e-51 179
MS.gene013256.t1 AT1G09750 33.252 409 233 13 49 437 61 449 1.15e-50 177
MS.gene013256.t1 AT4G30030 35.000 360 204 14 91 436 76 419 2.23e-50 176
MS.gene013256.t1 AT3G12700 30.260 423 263 12 34 436 50 460 6.35e-50 176
MS.gene013256.t1 AT2G28220 33.889 360 199 13 93 439 67 400 5.19e-49 172
MS.gene013256.t1 AT3G59080 35.641 390 190 14 71 436 144 496 5.42e-48 172
MS.gene013256.t1 AT3G54400 33.333 342 201 11 93 424 88 412 3.24e-47 168
MS.gene013256.t1 AT2G23945 30.266 413 258 13 46 439 50 451 9.19e-47 167
MS.gene013256.t1 AT4G30040 33.898 354 208 16 93 431 85 427 6.66e-44 159
MS.gene013256.t1 AT1G66180 31.351 370 217 11 94 439 73 429 6.77e-44 159
MS.gene013256.t1 AT5G37540 32.713 376 208 12 94 439 81 441 8.29e-43 156
MS.gene013256.t1 AT5G45120 31.415 417 214 19 82 436 72 478 1.64e-41 154
MS.gene013256.t1 AT5G07030 27.059 425 266 13 36 437 52 455 1.48e-40 150
MS.gene013256.t1 AT2G39710 31.551 374 206 14 95 432 67 426 3.12e-39 147
MS.gene013256.t1 AT5G02190 31.183 372 210 15 102 437 82 443 4.02e-39 147
MS.gene013256.t1 AT5G43100 30.376 372 213 17 90 439 73 420 1.69e-38 145
MS.gene013256.t1 AT5G43100 30.376 372 213 17 90 439 73 420 8.39e-38 145
MS.gene013256.t1 AT3G50050 30.894 369 217 15 90 439 90 439 2.31e-36 141
MS.gene013256.t1 AT5G36260 26.897 435 263 17 31 437 27 434 5.97e-34 133
MS.gene013256.t1 AT1G05840 29.288 379 219 18 91 437 78 439 7.79e-33 130
MS.gene013256.t1 AT1G65240 26.941 438 264 18 27 437 19 427 4.30e-31 125
MS.gene013256.t1 AT2G36670 27.187 423 257 20 50 437 52 458 4.38e-31 125
MS.gene013256.t1 AT5G22850 29.443 377 222 17 91 437 79 441 1.30e-30 124
MS.gene013256.t1 AT2G36670 26.869 428 257 20 50 437 52 463 2.40e-30 123
MS.gene013256.t1 AT2G36670 28.533 375 222 19 93 437 34 392 6.11e-30 121
MS.gene013256.t1 AT2G36670 28.533 375 222 19 93 437 34 392 6.11e-30 121
MS.gene013256.t1 AT3G02740 26.744 430 258 20 37 437 39 440 5.92e-29 119
MS.gene013256.t1 AT1G08210 29.412 374 223 16 91 437 82 441 1.42e-28 117
MS.gene013256.t1 AT1G08210 29.412 374 223 16 91 437 116 475 2.06e-28 117
MS.gene013256.t1 AT1G08210 29.412 374 223 16 91 437 130 489 2.49e-28 117
MS.gene013256.t1 AT3G02740 27.346 373 226 17 93 437 10 365 4.75e-28 115
MS.gene013256.t1 AT1G08210 30.746 335 195 15 91 402 82 402 5.94e-25 107
MS.gene013256.t1 AT4G33490 27.475 404 224 22 76 439 40 414 1.39e-24 105
MS.gene013256.t1 AT3G25700 35.065 154 87 4 82 222 69 222 2.38e-24 103
MS.gene013256.t1 AT3G25700 31.111 135 87 3 307 436 214 347 2.95e-15 77.4
MS.gene013256.t1 AT1G77480 29.925 401 213 24 79 439 50 422 1.88e-23 102
MS.gene013256.t1 AT1G77480 29.925 401 213 24 79 439 50 422 1.92e-23 102
MS.gene013256.t1 AT4G12920 26.952 397 250 14 56 437 17 388 1.45e-22 99.4
MS.gene013256.t1 AT4G16563 27.021 433 224 17 85 439 76 494 5.97e-22 98.6
MS.gene013256.t1 AT3G51350 25.843 356 219 12 93 424 102 436 6.76e-22 98.6
MS.gene013256.t1 AT4G35880 27.147 361 222 13 97 436 111 451 4.58e-21 95.9
MS.gene013256.t1 AT1G44130 27.649 387 211 23 91 439 47 402 3.96e-20 92.4
MS.gene013256.t1 AT4G35880 27.893 337 202 13 97 412 111 427 4.52e-20 92.4
MS.gene013256.t1 AT3G51360 26.486 370 225 14 93 437 89 436 1.31e-19 91.3
MS.gene013256.t1 AT3G51360 26.776 366 221 14 97 437 103 446 1.58e-19 91.3
MS.gene013256.t1 AT3G51360 26.776 366 221 14 97 437 9 352 1.67e-19 90.5
MS.gene013256.t1 AT2G17760 26.359 368 225 14 93 436 104 449 5.92e-19 89.7
MS.gene013256.t1 AT3G51330 25.641 351 219 11 97 424 106 437 6.40e-19 89.4
MS.gene013256.t1 AT4G33490 27.851 377 208 22 76 419 37 382 7.01e-19 88.6
MS.gene013256.t1 AT1G49050 25.604 414 253 19 65 438 172 570 7.92e-19 89.4
MS.gene013256.t1 AT3G51330 25.641 351 219 11 97 424 106 437 8.37e-19 89.4
MS.gene013256.t1 AT3G42550 24.658 365 203 13 93 437 78 390 8.47e-19 88.6
MS.gene013256.t1 AT3G51330 27.213 305 180 11 97 378 106 391 1.28e-18 88.2
MS.gene013256.t1 AT5G24820 25.469 373 226 12 95 436 48 399 7.12e-18 85.5
MS.gene013256.t1 AT1G49050 24.934 381 238 16 93 438 30 397 1.08e-17 85.1
MS.gene013256.t1 AT1G49050 27.027 296 187 13 65 342 172 456 1.86e-17 84.7
MS.gene013256.t1 AT1G03230 26.234 385 225 19 92 431 44 414 5.25e-17 83.2
MS.gene013256.t1 AT3G51360 27.439 328 193 13 97 401 103 408 1.18e-16 82.0
MS.gene013256.t1 AT3G51360 27.439 328 193 13 97 401 93 398 1.19e-16 82.0
MS.gene013256.t1 AT3G51360 25.323 387 231 15 17 378 19 372 1.21e-14 75.9
MS.gene013256.t1 AT3G51360 25.323 387 231 15 17 378 29 382 1.22e-14 75.9
MS.gene013256.t1 AT5G48430 25.271 277 180 8 179 431 115 388 2.16e-13 72.0
MS.gene013256.t1 AT3G51360 25.938 320 199 13 134 437 13 310 7.25e-13 70.1
MS.gene013256.t1 AT3G51360 25.938 320 199 13 134 437 13 310 7.25e-13 70.1

Find 84 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTAGATACTCACCGTTTC+CGG 0.192247 3.4:+87826039 None:intergenic
GGAACCCGCCTTGAGAAATT+TGG 0.230362 3.4:+87826224 None:intergenic
ATGATTGCTGATTCAAGTTT+AGG 0.247179 3.4:-87825140 MS.gene013256:CDS
ACACCATTGCATTTAGTCTT+TGG 0.261329 3.4:+87826113 None:intergenic
AATTTCTTACCCTGCAGTTT+TGG 0.262242 3.4:-87825993 MS.gene013256:CDS
CAAGGGTTTGAACAAGCTTC+AGG 0.279392 3.4:-87825665 MS.gene013256:CDS
GAAATTACCGGTCGATAAAT+CGG 0.298038 3.4:-87825279 MS.gene013256:CDS
AGGACGTGGTCCTTTGTCTT+TGG 0.305789 3.4:-87825630 MS.gene013256:CDS
CACTCTTCGCCATGTTGATT+CGG 0.307067 3.4:-87826197 MS.gene013256:CDS
TGCAATTATGTTTATTCATA+TGG 0.307345 3.4:-87825806 MS.gene013256:CDS
TCTAATGGAATGTCTATCTT+TGG 0.309215 3.4:-87825092 MS.gene013256:CDS
TCATTTCAAAGGTGGTGATT+TGG 0.324753 3.4:-87825183 MS.gene013256:CDS
GTCGATAAATCGGGTTCAAC+TGG 0.328905 3.4:-87825269 MS.gene013256:CDS
CTCTTATTGTTTAACCTCAA+TGG 0.332721 3.4:-87825582 MS.gene013256:CDS
GCTTCAGGGTTGGTTGGATT+AGG 0.351605 3.4:-87825650 MS.gene013256:CDS
AAAACTGCAGGGTAAGAAAT+TGG 0.351817 3.4:+87825995 None:intergenic
GGTGGTGTAATTATTGATTC+TGG 0.361626 3.4:-87825368 MS.gene013256:CDS
AGGTGTTGCTTGTTTAGCTA+TGG 0.362086 3.4:-87825120 MS.gene013256:CDS
GGTAGTGAAACATTCACTTT+TGG 0.375982 3.4:-87825755 MS.gene013256:CDS
AACCATTGTTGCTGCAAGTT+GGG 0.389462 3.4:+87825828 None:intergenic
TTAGCTATGGGAGCTTCTAA+TGG 0.390722 3.4:-87825107 MS.gene013256:CDS
GCGTTCAACATGGCATCAAC+CGG 0.402130 3.4:-87826150 MS.gene013256:CDS
TCACCGTTTCCGGCGTGAAC+CGG 0.417313 3.4:+87826049 None:intergenic
AAATGTTAATGCCACAAAAC+AGG 0.432893 3.4:-87825519 MS.gene013256:CDS
ACTCTTCGCCATGTTGATTC+GGG 0.433466 3.4:-87826196 MS.gene013256:CDS
GGTGTTGCTTGTTTAGCTAT+GGG 0.444613 3.4:-87825119 MS.gene013256:CDS
TACATCTCAAACGAAATTAC+CGG 0.459448 3.4:-87825291 MS.gene013256:CDS
TGCAATGGATTTGTAATAAG+TGG 0.466557 3.4:+87825488 None:intergenic
ATACAGGAAGTGATCTCATA+TGG 0.474114 3.4:-87825970 MS.gene013256:CDS
CCTCTTGATGTTGAGGATGT+TGG 0.474796 3.4:+87826262 None:intergenic
CGGAAACGGTGAGTATCTAA+TGG 0.475311 3.4:-87826038 MS.gene013256:CDS
ATCACCAAATTTCTCAAGGC+GGG 0.484091 3.4:-87826228 MS.gene013256:CDS
TTTGGAGTCGGCTATGTCCC+CGG 0.492840 3.4:+87826131 None:intergenic
CAACCATTGTTGCTGCAAGT+TGG 0.494678 3.4:+87825827 None:intergenic
ACTACATGAGAGTTTAGAAA+AGG 0.496166 3.4:+87825877 None:intergenic
CTTCCTGTATCCAAAACTGC+AGG 0.502993 3.4:+87825983 None:intergenic
TCACTTGAAGGTATATCTGT+TGG 0.504731 3.4:-87825446 MS.gene013256:CDS
CAACATGGCGAAGAGTGATT+CGG 0.510181 3.4:+87826203 None:intergenic
GCACCGGTTCACGCCGGAAA+CGG 0.511002 3.4:-87826052 MS.gene013256:CDS
TGAGAGTTTAGAAAAGGTTG+AGG 0.520561 3.4:+87825883 None:intergenic
AAGGGTTTGAACAAGCTTCA+GGG 0.524466 3.4:-87825664 MS.gene013256:CDS
GAACAAGCTTCAGGGTTGGT+TGG 0.529767 3.4:-87825656 MS.gene013256:CDS
AAATTACCGGTCGATAAATC+GGG 0.532183 3.4:-87825278 MS.gene013256:CDS
CGCCTTGAGAAATTTGGTGA+TGG 0.533286 3.4:+87826230 None:intergenic
AGAAATTGGTGGTGTTCCAA+TGG 0.538618 3.4:+87826009 None:intergenic
CGTTCAACATGGCATCAACC+GGG 0.541177 3.4:-87826149 MS.gene013256:CDS
TATCTAATGGAATTATCCAT+TGG 0.541639 3.4:-87826025 MS.gene013256:CDS
TATTCAACAACAAAATATGT+TGG 0.544631 3.4:-87825066 MS.gene013256:CDS
GGTGGTGATTTGGAATTGCC+TGG 0.558833 3.4:-87825173 MS.gene013256:CDS
AACTGAGGATTCATCTCAAG+TGG 0.559058 3.4:-87826077 MS.gene013256:CDS
GGGTTGGTTGGATTAGGACG+TGG 0.561420 3.4:-87825644 MS.gene013256:CDS
TCCCCAACTTGCAGCAACAA+TGG 0.562844 3.4:-87825830 MS.gene013256:CDS
GTTGAACCCGATTTATCGAC+CGG 0.563118 3.4:+87825272 None:intergenic
TGTCATGTTGGTGACACTAC+TGG 0.564089 3.4:-87826305 None:intergenic
TTCATCTCAAGTGGAAGCAC+CGG 0.564417 3.4:-87826068 MS.gene013256:CDS
GTTTGAACAAGCTTCAGGGT+TGG 0.567318 3.4:-87825660 MS.gene013256:CDS
TTGCATTTAGTCTTTGGAGT+CGG 0.574174 3.4:+87826119 None:intergenic
TTGTTGCTGCAAGTTGGGGA+AGG 0.579404 3.4:+87825833 None:intergenic
GAGGTTAGTGATGATGGAAG+TGG 0.582198 3.4:-87825389 MS.gene013256:CDS
ACCATTGTTGCTGCAAGTTG+GGG 0.588415 3.4:+87825829 None:intergenic
GAGAAACCCTCTTGATGTTG+AGG 0.588466 3.4:+87826255 None:intergenic
AATTGAGAAACCAAAGACAA+AGG 0.591149 3.4:+87825620 None:intergenic
AAACTCGAGCGCGTTCAACA+TGG 0.593524 3.4:-87826160 MS.gene013256:CDS
GGTTAATCATGATCTTCAGA+AGG 0.593723 3.4:-87825045 MS.gene013256:CDS
GGGAAGGTAAAGCATTGCAT+AGG 0.594254 3.4:+87825849 None:intergenic
AACCATCACCAAATTTCTCA+AGG 0.601116 3.4:-87826232 MS.gene013256:CDS
GGTTGTGGTGAAGACAATGA+AGG 0.605437 3.4:-87825689 MS.gene013256:CDS
CCAACATCCTCAACATCAAG+AGG 0.614340 3.4:-87826262 MS.gene013256:CDS
TGTTTGTAACATTGTGAACA+AGG 0.616147 3.4:+87825935 None:intergenic
ACTGCAGGGTAAGAAATTGG+TGG 0.619033 3.4:+87825998 None:intergenic
AGTTCTTGCCCGAATCAACA+TGG 0.641331 3.4:+87826188 None:intergenic
TTGAGATGAATCCTCAGTTG+TGG 0.642542 3.4:+87826081 None:intergenic
TTCCTGTATCCAAAACTGCA+GGG 0.643359 3.4:+87825984 None:intergenic
GTGGAAGCACCGGTTCACGC+CGG 0.647259 3.4:-87826058 MS.gene013256:CDS
GGTGAAGACAATGAAGGACA+AGG 0.647678 3.4:-87825683 MS.gene013256:CDS
ACTCCAAAGACTAAATGCAA+TGG 0.654657 3.4:-87826116 MS.gene013256:CDS
CACAACTTATTACATTGAGT+AGG 0.657341 3.4:+87825005 None:intergenic
GTTAGTGATGATGGAAGTGG+TGG 0.663606 3.4:-87825386 MS.gene013256:CDS
CAACATCCTCAACATCAAGA+GGG 0.669695 3.4:-87826261 MS.gene013256:CDS
GTTAGCATCATCCACAACTG+AGG 0.676686 3.4:-87826092 MS.gene013256:CDS
GTGAAGACAATGAAGGACAA+GGG 0.682228 3.4:-87825682 MS.gene013256:CDS
CATCACCAAATTTCTCAAGG+CGG 0.689470 3.4:-87826229 MS.gene013256:CDS
GTTCAACATGGCATCAACCG+GGG 0.699502 3.4:-87826148 MS.gene013256:CDS
GGTGATTATTCTATGACACA+AGG 0.711992 3.4:-87825785 MS.gene013256:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTAATTTTTCATTTCAA+AGG - chr3.4:87826111-87826130 MS.gene013256:CDS 10.0%
!!! TTGAAATGAAAAATTAATTT+TGG + chr3.4:87826111-87826130 None:intergenic 10.0%
!! AGTGAAATATAATAAAAAGA+TGG + chr3.4:87825844-87825863 None:intergenic 15.0%
!!! GAATTTTTTGGATTAAAAAT+TGG + chr3.4:87825403-87825422 None:intergenic 15.0%
!!! TTTTATTATATTTCACTTGA+AGG - chr3.4:87825847-87825866 MS.gene013256:CDS 15.0%
!! TATTCAACAACAAAATATGT+TGG - chr3.4:87826239-87826258 MS.gene013256:CDS 20.0%
!! TGCAATTATGTTTATTCATA+TGG - chr3.4:87825499-87825518 MS.gene013256:CDS 20.0%
!!! TTAATTTTTCATTTCAAAGG+TGG - chr3.4:87826114-87826133 MS.gene013256:CDS 20.0%
! CAAGTTTCTGTTAAAAACAT+TGG - chr3.4:87825589-87825608 MS.gene013256:CDS 25.0%
! TAATAAAAAGATGGTTGCAA+TGG + chr3.4:87825835-87825854 None:intergenic 25.0%
! TATCTAATGGAATTATCCAT+TGG - chr3.4:87825280-87825299 MS.gene013256:CDS 25.0%
! TTTGGATTAAAAATTGGTGT+TGG + chr3.4:87825397-87825416 None:intergenic 25.0%
!!! AAAGAAAGTGTTCTTTTGTT+AGG - chr3.4:87825754-87825773 MS.gene013256:CDS 25.0%
!!! CAATGTTTTTAACAGAAACT+TGG + chr3.4:87825591-87825610 None:intergenic 25.0%
!!! TCTGTTAAAAACATTGGTTT+TGG - chr3.4:87825595-87825614 MS.gene013256:CDS 25.0%
AAATGTTAATGCCACAAAAC+AGG - chr3.4:87825786-87825805 MS.gene013256:CDS 30.0%
AATTGAGAAACCAAAGACAA+AGG + chr3.4:87825688-87825707 None:intergenic 30.0%
ACTACATGAGAGTTTAGAAA+AGG + chr3.4:87825431-87825450 None:intergenic 30.0%
CACAACTTATTACATTGAGT+AGG + chr3.4:87826303-87826322 None:intergenic 30.0%
CTCTTATTGTTTAACCTCAA+TGG - chr3.4:87825723-87825742 MS.gene013256:CDS 30.0%
TACATCTCAAACGAAATTAC+CGG - chr3.4:87826014-87826033 MS.gene013256:CDS 30.0%
TCTAATGGAATGTCTATCTT+TGG - chr3.4:87826213-87826232 MS.gene013256:CDS 30.0%
TGTTTGTAACATTGTGAACA+AGG + chr3.4:87825373-87825392 None:intergenic 30.0%
! AATTGAGCAATCAACTTTTG+AGG - chr3.4:87825897-87825916 MS.gene013256:CDS 30.0%
! GCAATCATATAATTTTCACC+AGG + chr3.4:87826153-87826172 None:intergenic 30.0%
! TGCAATGGATTTGTAATAAG+TGG + chr3.4:87825820-87825839 None:intergenic 30.0%
! TGTTTTGTGGCATTAACATT+TGG + chr3.4:87825787-87825806 None:intergenic 30.0%
!! ATGATTGCTGATTCAAGTTT+AGG - chr3.4:87826165-87826184 MS.gene013256:CDS 30.0%
!! TCTATGACACAAGGTATTTT+AGG - chr3.4:87825529-87825548 MS.gene013256:CDS 30.0%
!!! AAAAACATTGGTTTTGGTTG+TGG - chr3.4:87825601-87825620 MS.gene013256:CDS 30.0%
!!! GAAAAGGTTGAGGAATTTTT+TGG + chr3.4:87825415-87825434 None:intergenic 30.0%
!!! TTTGTTTTAGTTTGCCATCA+GGG - chr3.4:87826067-87826086 MS.gene013256:CDS 30.0%
AAAACTGCAGGGTAAGAAAT+TGG + chr3.4:87825313-87825332 None:intergenic 35.0%
AAATTACCGGTCGATAAATC+GGG - chr3.4:87826027-87826046 MS.gene013256:CDS 35.0%
AACCATCACCAAATTTCTCA+AGG - chr3.4:87825073-87825092 MS.gene013256:CDS 35.0%
ACTCCAAAGACTAAATGCAA+TGG - chr3.4:87825189-87825208 MS.gene013256:CDS 35.0%
ATACAGGAAGTGATCTCATA+TGG - chr3.4:87825335-87825354 MS.gene013256:CDS 35.0%
GAAATTACCGGTCGATAAAT+CGG - chr3.4:87826026-87826045 MS.gene013256:CDS 35.0%
GGTGATTATTCTATGACACA+AGG - chr3.4:87825520-87825539 MS.gene013256:CDS 35.0%
GGTTAATCATGATCTTCAGA+AGG - chr3.4:87826260-87826279 MS.gene013256:CDS 35.0%
TCATTTCAAAGGTGGTGATT+TGG - chr3.4:87826122-87826141 MS.gene013256:CDS 35.0%
TGAGAGTTTAGAAAAGGTTG+AGG + chr3.4:87825425-87825444 None:intergenic 35.0%
! AATTTCTTACCCTGCAGTTT+TGG - chr3.4:87825312-87825331 MS.gene013256:CDS 35.0%
! ACACCATTGCATTTAGTCTT+TGG + chr3.4:87825195-87825214 None:intergenic 35.0%
! GGTAGTGAAACATTCACTTT+TGG - chr3.4:87825550-87825569 MS.gene013256:CDS 35.0%
! TCACTTGAAGGTATATCTGT+TGG - chr3.4:87825859-87825878 MS.gene013256:CDS 35.0%
! TTCTTTTGTGTCATCCATTG+AGG + chr3.4:87825740-87825759 None:intergenic 35.0%
!! GGTGGTGTAATTATTGATTC+TGG - chr3.4:87825937-87825956 MS.gene013256:CDS 35.0%
!! TTGCATTTAGTCTTTGGAGT+CGG + chr3.4:87825189-87825208 None:intergenic 35.0%
!!! ACTTTTGAGGTTAGTGATGA+TGG - chr3.4:87825910-87825929 MS.gene013256:CDS 35.0%
!!! GTTTGTTTTAGTTTGCCATC+AGG - chr3.4:87826066-87826085 MS.gene013256:CDS 35.0%
AACCATTGTTGCTGCAAGTT+GGG + chr3.4:87825480-87825499 None:intergenic 40.0%
AACTGAGGATTCATCTCAAG+TGG - chr3.4:87825228-87825247 MS.gene013256:CDS 40.0%
AAGGGTTTGAACAAGCTTCA+GGG - chr3.4:87825641-87825660 MS.gene013256:CDS 40.0%
ATCACCAAATTTCTCAAGGC+GGG - chr3.4:87825077-87825096 MS.gene013256:CDS 40.0%
CAACATCCTCAACATCAAGA+GGG - chr3.4:87825044-87825063 MS.gene013256:CDS 40.0%
CATCACCAAATTTCTCAAGG+CGG - chr3.4:87825076-87825095 MS.gene013256:CDS 40.0%
CATTAGATACTCACCGTTTC+CGG + chr3.4:87825269-87825288 None:intergenic 40.0%
GTGAAGACAATGAAGGACAA+GGG - chr3.4:87825623-87825642 MS.gene013256:CDS 40.0%
TTAGCTATGGGAGCTTCTAA+TGG - chr3.4:87826198-87826217 MS.gene013256:CDS 40.0%
TTCCTGTATCCAAAACTGCA+GGG + chr3.4:87825324-87825343 None:intergenic 40.0%
TTGAGATGAATCCTCAGTTG+TGG + chr3.4:87825227-87825246 None:intergenic 40.0%
! TCAACTTCTGTTTTCCCTGA+TGG + chr3.4:87826084-87826103 None:intergenic 40.0%
! TGGAGTTGTTACCTGTTTTG+TGG + chr3.4:87825800-87825819 None:intergenic 40.0%
!! AGAAATTGGTGGTGTTCCAA+TGG + chr3.4:87825299-87825318 None:intergenic 40.0%
!! AGGTGTTGCTTGTTTAGCTA+TGG - chr3.4:87826185-87826204 MS.gene013256:CDS 40.0%
!! GGTGTTGCTTGTTTAGCTAT+GGG - chr3.4:87826186-87826205 MS.gene013256:CDS 40.0%
ACCATTGTTGCTGCAAGTTG+GGG + chr3.4:87825479-87825498 None:intergenic 45.0%
ACTGCAGGGTAAGAAATTGG+TGG + chr3.4:87825310-87825329 None:intergenic 45.0%
AGTTCTTGCCCGAATCAACA+TGG + chr3.4:87825120-87825139 None:intergenic 45.0%
CAACATGGCGAAGAGTGATT+CGG + chr3.4:87825105-87825124 None:intergenic 45.0%
CAACCATTGTTGCTGCAAGT+TGG + chr3.4:87825481-87825500 None:intergenic 45.0%
CAAGGGTTTGAACAAGCTTC+AGG - chr3.4:87825640-87825659 MS.gene013256:CDS 45.0%
CCAACATCCTCAACATCAAG+AGG - chr3.4:87825043-87825062 MS.gene013256:CDS 45.0%
CCTCTTGATGTTGAGGATGT+TGG + chr3.4:87825046-87825065 None:intergenic 45.0%
CGGAAACGGTGAGTATCTAA+TGG - chr3.4:87825267-87825286 MS.gene013256:CDS 45.0%
CTTCCTGTATCCAAAACTGC+AGG + chr3.4:87825325-87825344 None:intergenic 45.0%
GAGAAACCCTCTTGATGTTG+AGG + chr3.4:87825053-87825072 None:intergenic 45.0%
GGTGAAGACAATGAAGGACA+AGG - chr3.4:87825622-87825641 MS.gene013256:CDS 45.0%
GGTTGTGGTGAAGACAATGA+AGG - chr3.4:87825616-87825635 MS.gene013256:CDS 45.0%
GTCGATAAATCGGGTTCAAC+TGG - chr3.4:87826036-87826055 MS.gene013256:CDS 45.0%
GTTAGCATCATCCACAACTG+AGG - chr3.4:87825213-87825232 MS.gene013256:CDS 45.0%
GTTGAACCCGATTTATCGAC+CGG + chr3.4:87826036-87826055 None:intergenic 45.0%
GTTTGAACAAGCTTCAGGGT+TGG - chr3.4:87825645-87825664 MS.gene013256:CDS 45.0%
! CACTCTTCGCCATGTTGATT+CGG - chr3.4:87825108-87825127 MS.gene013256:CDS 45.0%
! CGCCTTGAGAAATTTGGTGA+TGG + chr3.4:87825078-87825097 None:intergenic 45.0%
! TTCATCTCAAGTGGAAGCAC+CGG - chr3.4:87825237-87825256 MS.gene013256:CDS 45.0%
!! ACTCTTCGCCATGTTGATTC+GGG - chr3.4:87825109-87825128 MS.gene013256:CDS 45.0%
!! GAGGTTAGTGATGATGGAAG+TGG - chr3.4:87825916-87825935 MS.gene013256:CDS 45.0%
!! GGGAAGGTAAAGCATTGCAT+AGG + chr3.4:87825459-87825478 None:intergenic 45.0%
!! GTTAGTGATGATGGAAGTGG+TGG - chr3.4:87825919-87825938 MS.gene013256:CDS 45.0%
!!! TACCCTGCAGTTTTGGATAC+AGG - chr3.4:87825319-87825338 MS.gene013256:CDS 45.0%
AAACTCGAGCGCGTTCAACA+TGG - chr3.4:87825145-87825164 MS.gene013256:CDS 50.0%
AGGACGTGGTCCTTTGTCTT+TGG - chr3.4:87825675-87825694 MS.gene013256:CDS 50.0%
CGTTCAACATGGCATCAACC+GGG - chr3.4:87825156-87825175 MS.gene013256:CDS 50.0%
GCGTTCAACATGGCATCAAC+CGG - chr3.4:87825155-87825174 MS.gene013256:CDS 50.0%
GGAACCCGCCTTGAGAAATT+TGG + chr3.4:87825084-87825103 None:intergenic 50.0%
GTTCAACATGGCATCAACCG+GGG - chr3.4:87825157-87825176 MS.gene013256:CDS 50.0%
TCCCCAACTTGCAGCAACAA+TGG - chr3.4:87825475-87825494 MS.gene013256:CDS 50.0%
! GAACAAGCTTCAGGGTTGGT+TGG - chr3.4:87825649-87825668 MS.gene013256:CDS 50.0%
! GGTGGTGATTTGGAATTGCC+TGG - chr3.4:87826132-87826151 MS.gene013256:CDS 50.0%
! TTGTTGCTGCAAGTTGGGGA+AGG + chr3.4:87825475-87825494 None:intergenic 50.0%
!! GCTTCAGGGTTGGTTGGATT+AGG - chr3.4:87825655-87825674 MS.gene013256:CDS 50.0%
! TTTGGAGTCGGCTATGTCCC+CGG + chr3.4:87825177-87825196 None:intergenic 55.0%
!! GGGTTGGTTGGATTAGGACG+TGG - chr3.4:87825661-87825680 MS.gene013256:CDS 55.0%
TCACCGTTTCCGGCGTGAAC+CGG + chr3.4:87825259-87825278 None:intergenic 60.0%
!! GCACCGGTTCACGCCGGAAA+CGG - chr3.4:87825253-87825272 MS.gene013256:CDS 65.0%
!! GTGGAAGCACCGGTTCACGC+CGG - chr3.4:87825247-87825266 MS.gene013256:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 87825004 87826323 87825004 ID=MS.gene013256
chr3.4 mRNA 87825004 87826323 87825004 ID=MS.gene013256.t1;Parent=MS.gene013256
chr3.4 exon 87825004 87826323 87825004 ID=MS.gene013256.t1.exon1;Parent=MS.gene013256.t1
chr3.4 CDS 87825004 87826323 87825004 ID=cds.MS.gene013256.t1;Parent=MS.gene013256.t1
Gene Sequence

>MS.gene013256

ATGTTGGTGACACTACTGGTAACAATTCTCTTTGTTCATCCAACATCCTCAACATCAAGAGGGTTTCTCAACCATCACCAAATTTCTCAAGGCGGGTTCCGAATCACTCTTCGCCATGTTGATTCGGGCAAGAACTTGACTAAACTCGAGCGCGTTCAACATGGCATCAACCGGGGACATAGCCGACTCCAAAGACTAAATGCAATGGTGTTAGCATCATCCACAACTGAGGATTCATCTCAAGTGGAAGCACCGGTTCACGCCGGAAACGGTGAGTATCTAATGGAATTATCCATTGGAACACCACCAATTTCTTACCCTGCAGTTTTGGATACAGGAAGTGATCTCATATGGACTCAATGTGAACCTTGTTCACAATGTTACAAACAACCAACACCAATTTTTAATCCAAAAAATTCCTCAACCTTTTCTAAACTCTCATGTAGTAGCAACCTATGCAATGCTTTACCTTCCCCAACTTGCAGCAACAATGGTTGCAATTATGTTTATTCATATGGTGATTATTCTATGACACAAGGTATTTTAGGTAGTGAAACATTCACTTTTGGTGATGATAAAAAAAACCAAGTTTCTGTTAAAAACATTGGTTTTGGTTGTGGTGAAGACAATGAAGGACAAGGGTTTGAACAAGCTTCAGGGTTGGTTGGATTAGGACGTGGTCCTTTGTCTTTGGTTTCTCAATTAAAAGAACAAAAATTCTCTTATTGTTTAACCTCAATGGATGACACAAAAGAAAGTGTTCTTTTGTTAGGTTCTCTTCCAAATGTTAATGCCACAAAACAGGTAACAACTCCACTTATTACAAATCCATTGCAACCATCTTTTTATTATATTTCACTTGAAGGTATATCTGTTGGTGACACAAAATTGTCAATTGAGCAATCAACTTTTGAGGTTAGTGATGATGGAAGTGGTGGTGTAATTATTGATTCTGGCACAACAATTACTTACATTGAAGAGAATGCTTTTGATTCATTGAAGAAAGAGTTTACATCTCAAACGAAATTACCGGTCGATAAATCGGGTTCAACTGGATTAGATGTTTGTTTTAGTTTGCCATCAGGGAAAACAGAAGTTGAAATTCCAAAATTAATTTTTCATTTCAAAGGTGGTGATTTGGAATTGCCTGGTGAAAATTATATGATTGCTGATTCAAGTTTAGGTGTTGCTTGTTTAGCTATGGGAGCTTCTAATGGAATGTCTATCTTTGGTAATATTCAACAACAAAATATGTTGGTTAATCATGATCTTCAGAAGGAAACTATTACTTTTGTTCCTACTCAATGTAATAAGTTGTGA
Protein sequence

>MS.gene013256.t1

MLVTLLVTILFVHPTSSTSRGFLNHHQISQGGFRITLRHVDSGKNLTKLERVQHGINRGHSRLQRLNAMVLASSTTEDSSQVEAPVHAGNGEYLMELSIGTPPISYPAVLDTGSDLIWTQCEPCSQCYKQPTPIFNPKNSSTFSKLSCSSNLCNALPSPTCSNNGCNYVYSYGDYSMTQGILGSETFTFGDDKKNQVSVKNIGFGCGEDNEGQGFEQASGLVGLGRGPLSLVSQLKEQKFSYCLTSMDDTKESVLLLGSLPNVNATKQVTTPLITNPLQPSFYYISLEGISVGDTKLSIEQSTFEVSDDGSGGVIIDSGTTITYIEENAFDSLKKEFTSQTKLPVDKSGSTGLDVCFSLPSGKTEVEIPKLIFHFKGGDLELPGENYMIADSSLGVACLAMGASNGMSIFGNIQQQNMLVNHDLQKETITFVPTQCNKL