AlfalfaGEDB | MS.gene018902

M. sativa cultivar XinJiangDaYe / MS.gene018902

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008146 GO:0016740 GO:0016782
PFAM: PF00685.30

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene018902.t1	XP_013459262.1	95.2	334	16	0	1	334	1	334	2.70E-187	664.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene018902.t1	P52839	49.8	327	158	2	8	334	4	324	9.5e-93	341.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene018902.t1	A0A072UW61	95.2	334	16	0	1	334	1	334	1.9e-187	664.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene018902	MS.gene55382	PPI
MS.gene018902	MS.gene99693	PPI
MS.gene018902	MS.gene53169	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene018902.t1	MTR_3g435130	95.210	334	16	0	1	334	1	334	0.0	656
MS.gene018902.t1	MTR_0071s0010	73.030	330	89	0	5	334	4	333	0.0	516
MS.gene018902.t1	MTR_3g046670	73.030	330	89	0	5	334	4	333	0.0	516
MS.gene018902.t1	MTR_8g066220	72.156	334	92	1	1	334	1	333	0.0	511
MS.gene018902.t1	MTR_8g066210	69.760	334	85	2	1	334	1	318	9.34e-172	479
MS.gene018902.t1	MTR_3g013500	50.943	318	155	1	17	334	21	337	3.81e-116	339
MS.gene018902.t1	MTR_7g007710	48.910	321	158	3	18	334	14	332	1.41e-113	333
MS.gene018902.t1	MTR_7g007680	47.500	320	159	4	18	334	20	333	1.23e-108	322
MS.gene018902.t1	MTR_8g040820	47.436	312	159	4	27	334	34	344	2.31e-105	312
MS.gene018902.t1	MTR_8g035720	46.505	329	172	2	10	334	78	406	3.26e-104	311
MS.gene018902.t1	MTR_8g040490	43.614	321	177	3	17	334	24	343	4.88e-98	295
MS.gene018902.t1	MTR_8g066280	70.745	188	55	0	147	334	22	209	6.65e-98	288
MS.gene018902.t1	MTR_8g040430	41.246	337	182	5	3	334	20	345	8.73e-95	285
MS.gene018902.t1	MTR_8g040520	46.349	315	161	4	17	327	24	334	1.64e-93	282
MS.gene018902.t1	MTR_8g041790	43.533	317	155	6	23	334	28	325	3.20e-91	276
MS.gene018902.t1	MTR_3g434790	66.667	192	64	0	143	334	43	234	8.02e-91	271
MS.gene018902.t1	MTR_3g434790	73.684	57	14	1	1	56	1	57	7.39e-22	92.8
MS.gene018902.t1	MTR_8g014380	42.662	293	127	5	16	306	22	275	1.01e-78	242
MS.gene018902.t1	MTR_8g040250	46.154	247	126	4	85	327	45	288	2.09e-76	237
MS.gene018902.t1	MTR_8g040820	48.402	219	109	3	120	334	27	245	7.42e-73	226
MS.gene018902.t1	MTR_8g040430	42.922	219	121	2	120	334	27	245	7.28e-64	203
MS.gene018902.t1	MTR_8g040730	49.171	181	83	5	78	253	39	215	4.12e-45	161
MS.gene018902.t1	MTR_8g040730	38.278	209	101	5	17	223	338	520	5.34e-39	145
MS.gene018902.t1	MTR_8g040710	45.638	149	79	1	178	324	134	282	7.12e-38	136
MS.gene018902.t1	MTR_8g066190	54.206	107	31	1	228	334	4	92	1.77e-29	108
MS.gene018902.t1	MTR_8g040315	41.985	131	75	1	204	334	3	132	2.89e-25	99.4
MS.gene018902.t1	MTR_8g066200	80.556	36	7	0	152	187	25	60	4.75e-15	69.3
MS.gene018902.t1	MTR_8g040410	48.333	60	30	1	66	124	21	80	1.41e-12	64.7
MS.gene018902.t1	MTR_8g040410	33.708	89	41	1	218	306	75	145	3.80e-11	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene018902.t1	AT5G07010	48.000	325	167	2	11	334	33	356	1.73e-111	328
MS.gene018902.t1	AT3G45070	50.156	321	157	3	15	334	5	323	4.75e-111	326
MS.gene018902.t1	AT3G45070	50.156	321	157	3	15	334	10	328	5.61e-111	326
MS.gene018902.t1	AT3G45070	50.156	321	157	3	15	334	37	355	8.02e-111	327
MS.gene018902.t1	AT2G03760	49.541	327	159	2	8	334	4	324	3.10e-110	324
MS.gene018902.t1	AT5G07000	47.352	321	165	3	17	334	25	344	1.89e-104	310
MS.gene018902.t1	AT3G45080	49.379	322	159	4	15	334	10	329	3.64e-104	308
MS.gene018902.t1	AT2G03770	47.516	322	160	2	13	334	10	322	2.68e-102	303
MS.gene018902.t1	AT1G13420	50.000	312	148	4	25	334	26	331	8.14e-102	303
MS.gene018902.t1	AT2G03750	45.679	324	173	3	11	334	30	350	8.27e-97	291
MS.gene018902.t1	AT5G43690	45.783	332	169	6	9	334	5	331	6.42e-94	283
MS.gene018902.t1	AT4G26280	46.984	315	146	3	15	327	7	302	1.45e-93	281
MS.gene018902.t1	AT1G28170	46.626	326	167	6	11	334	6	326	4.75e-93	280
MS.gene018902.t1	AT1G18590	44.969	318	161	5	22	332	31	341	9.35e-92	278
MS.gene018902.t1	AT2G14920	45.714	315	163	4	25	334	22	333	3.00e-91	276
MS.gene018902.t1	AT1G74100	43.711	318	165	5	22	332	23	333	6.42e-89	270
MS.gene018902.t1	AT1G13430	44.681	329	160	5	25	334	26	351	8.15e-84	257
MS.gene018902.t1	AT1G74090	44.720	322	159	7	22	331	35	349	4.22e-81	250
MS.gene018902.t1	AT2G27570	40.938	320	133	4	15	334	10	273	1.78e-78	241

Find 0 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	GAATATTTTGATATTTTTTG+CGG	+	23447:128637-128656	None:intergenic	15.0%
!!!	AGCTTTTTCAAATGAAAATA+AGG	+	23447:129076-129095	None:intergenic	20.0%
!!!	CTTTTGATAAATTTACTAGA+GGG	-	23447:128942-128961	MS.gene018902:CDS	20.0%
!!!	TCTTTTGATAAATTTACTAG+AGG	-	23447:128941-128960	MS.gene018902:CDS	20.0%
!!!	TTTTGATAAATTTACTAGAG+GGG	-	23447:128943-128962	MS.gene018902:CDS	20.0%
!	AAGAGAAATTGGTTAAACAT+GGG	-	23447:129323-129342	MS.gene018902:CDS	25.0%
!	TAAGAGGATATCTGTATTAA+GGG	+	23447:128555-128574	None:intergenic	25.0%
!	TTATCAAAAGACTCTTCTAA+TGG	+	23447:128932-128951	None:intergenic	25.0%
!!	TTCATCAATATCAAGGTTTT+TGG	-	23447:128477-128496	MS.gene018902:CDS	25.0%
	AACCTTGATATTGATGAAAG+TGG	+	23447:128475-128494	None:intergenic	30.0%
	ACCAAAGATGAAGAATCTAA+AGG	-	23447:129121-129140	MS.gene018902:CDS	30.0%
	ACCTTGATATTGATGAAAGT+GGG	+	23447:128474-128493	None:intergenic	30.0%
	CAAACCTTACCATTAGAAAA+AGG	-	23447:128437-128456	MS.gene018902:CDS	30.0%
	CAAAGATGAAGAATCTAAAG+GGG	-	23447:129123-129142	MS.gene018902:CDS	30.0%
	CAGTGTAATTGAAGAGAAAT+TGG	-	23447:129312-129331	MS.gene018902:CDS	30.0%
	CCAAAGATGAAGAATCTAAA+GGG	-	23447:129122-129141	MS.gene018902:CDS	30.0%
	CTAAGAGGATATCTGTATTA+AGG	+	23447:128556-128575	None:intergenic	30.0%
	CTTACCATTAGAAAAAGGAT+GGG	-	23447:128442-128461	MS.gene018902:CDS	30.0%
	GAAGAGAAATTGGTTAAACA+TGG	-	23447:129322-129341	MS.gene018902:CDS	30.0%
	TTGGAGAGGATAATGAATTT+AGG	+	23447:128754-128773	None:intergenic	30.0%
	TTTGACAAGATAAAACACCT+TGG	+	23447:128520-128539	None:intergenic	30.0%
!	AAAAGAGTCAAGTTGTAAAG+TGG	-	23447:128820-128839	MS.gene018902:CDS	30.0%
!	CTTGACTCTTTTACAGATTT+TGG	+	23447:128812-128831	None:intergenic	30.0%
!	GAGGATAATGAATTTAGGTT+AGG	+	23447:128749-128768	None:intergenic	30.0%
!!	AACAAAGCTTATTTTCGTTG+TGG	-	23447:129235-129254	MS.gene018902:CDS	30.0%
!!	CAATATCAAGGTTTTTGGAT+TGG	-	23447:128482-128501	MS.gene018902:CDS	30.0%
!!	GATTTTGGCAACAAATCATA+TGG	+	23447:128797-128816	None:intergenic	30.0%
!!!	GAAAAAGCTTGCTGAATTTT+TGG	-	23447:129087-129106	MS.gene018902:CDS	30.0%
	AACTTGGAGGTTAATAAGAC+TGG	-	23447:129190-129209	MS.gene018902:CDS	35.0%
	AAGCTTGGAAAGATCAAACA+AGG	-	23447:129015-129034	MS.gene018902:CDS	35.0%
	AAGGTAAGATCACAAAGTAG+TGG	-	23447:128899-128918	MS.gene018902:CDS	35.0%
	ACCCACTTTCATCAATATCA+AGG	-	23447:128470-128489	MS.gene018902:CDS	35.0%
	ACTATGGCATTTCTCAAACA+AGG	-	23447:128880-128899	MS.gene018902:CDS	35.0%
	ACTTGGAGGTTAATAAGACT+GGG	-	23447:129191-129210	MS.gene018902:CDS	35.0%
	AGGATATTGGAAAGAAAGCT+TGG	-	23447:129000-129019	MS.gene018902:CDS	35.0%
	ATTTAGGAGTGGTAACTAAG+AGG	+	23447:128571-128590	None:intergenic	35.0%
	CCCTTTAGATTCTTCATCTT+TGG	+	23447:129125-129144	None:intergenic	35.0%
	CCTTACCATTAGAAAAAGGA+TGG	-	23447:128441-128460	MS.gene018902:CDS	35.0%
	GATTCTTCATCTTTGGTGAA+TGG	+	23447:129118-129137	None:intergenic	35.0%
	GGGATCATGTATTAGGATAT+TGG	-	23447:128987-129006	MS.gene018902:CDS	35.0%
	TAAAGATCAACCTCCAAGAA+GGG	+	23447:128707-128726	None:intergenic	35.0%
	TTAGTTACCACTCCTAAATC+AGG	-	23447:128572-128591	MS.gene018902:CDS	35.0%
!	AAGACACTTTTGCTTCACTA+TGG	-	23447:128864-128883	MS.gene018902:CDS	35.0%
!	CCATCCTTTTTCTAATGGTA+AGG	+	23447:128444-128463	None:intergenic	35.0%
!	CTAACCCATCCTTTTTCTAA+TGG	+	23447:128449-128468	None:intergenic	35.0%
!	TCATCTTCTCGCAAGTATTT+TGG	+	23447:128389-128408	None:intergenic	35.0%
!!	AGTGAAGCAAAAGTGTCTTT+AGG	+	23447:128863-128882	None:intergenic	35.0%
	AAGAAGGGAACAAGAACATG+AGG	+	23447:128692-128711	None:intergenic	40.0%
	AGAAGGGAACAAGAACATGA+GGG	+	23447:128691-128710	None:intergenic	40.0%
	AGATGAAGAATCTAAAGGGG+TGG	-	23447:129126-129145	MS.gene018902:CDS	40.0%
	ATGTGTGTTGCAAAGAGTCT+TGG	+	23447:128773-128792	None:intergenic	40.0%
	ATTGGCACAAGAATTCTCCA+AGG	-	23447:128500-128519	MS.gene018902:CDS	40.0%
	CCTAATACATGATCCCAACA+GGG	+	23447:128983-129002	None:intergenic	40.0%
	GTAAAGATCAACCTCCAAGA+AGG	+	23447:128708-128727	None:intergenic	40.0%
	TACTTGCGAGAAGATGACTT+AGG	-	23447:128392-128411	MS.gene018902:CDS	40.0%
	TCATGTTCTTGTTCCCTTCT+TGG	-	23447:128691-128710	MS.gene018902:CDS	40.0%
	TCCTAATACATGATCCCAAC+AGG	+	23447:128984-129003	None:intergenic	40.0%
	TGACTTAGGCAAAGAATGCA+AGG	-	23447:128406-128425	MS.gene018902:CDS	40.0%
	TTTGCGGTTTATCAAAGCGA+AGG	+	23447:128621-128640	None:intergenic	40.0%
!	TATTTTCGTTGTGGCCAAGT+TGG	-	23447:129244-129263	MS.gene018902:CDS	40.0%
!!	AAGACTGGGAAAGTGTCATT+TGG	-	23447:129205-129224	MS.gene018902:CDS	40.0%
!!	TTTTGAGAAGCTGAGCAACT+TGG	-	23447:129174-129193	MS.gene018902:CDS	40.0%
	AGCCAAGTGGTACCTGATTT+AGG	+	23447:128587-128606	None:intergenic	45.0%
	AGTGGTACCTGATTTAGGAG+TGG	+	23447:128582-128601	None:intergenic	45.0%
	CCCTGTTGGGATCATGTATT+AGG	-	23447:128980-128999	MS.gene018902:CDS	45.0%
	CTCCTAAATCAGGTACCACT+TGG	-	23447:128582-128601	MS.gene018902:CDS	45.0%
	TGAGGGTTTGTAGTGAGCAA+AGG	+	23447:128674-128693	None:intergenic	45.0%
	TGTTCTTGTTCCCTTCTTGG+AGG	-	23447:128694-128713	MS.gene018902:CDS	45.0%
	TGTTGCAAAGAGTCTTGGAG+AGG	+	23447:128768-128787	None:intergenic	45.0%
!	ACTAGAGGGGTCACTTTGTT+TGG	-	23447:128956-128975	MS.gene018902:CDS	45.0%
!	CACTTTGTTTGGACCCTGTT+GGG	-	23447:128967-128986	MS.gene018902:CDS	45.0%
!	GCTTTTCTCCATCTCCAACT+TGG	+	23447:129261-129280	None:intergenic	45.0%
!	GGTTAATGCTTTGAGCCAAG+TGG	+	23447:128600-128619	None:intergenic	45.0%
!	TCACTTTGTTTGGACCCTGT+TGG	-	23447:128966-128985	MS.gene018902:CDS	45.0%
	TGAGAAGCTGAGCAACTTGG+AGG	-	23447:129177-129196	MS.gene018902:CDS	50.0%
!	CGTTGTGGCCAAGTTGGAGA+TGG	-	23447:129250-129269	MS.gene018902:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
23447	gene	128353	129357	128353	ID=MS.gene018902
23447	mRNA	128353	129357	128353	ID=MS.gene018902.t1;Parent=MS.gene018902
23447	exon	128353	129357	128353	ID=MS.gene018902.t1.exon1;Parent=MS.gene018902.t1
23447	CDS	128353	129357	128353	ID=cds.MS.gene018902.t1;Parent=MS.gene018902.t1

Gene Sequence

>MS.gene018902

ATGGCAAAGTTAGGTGATGAAAACTCTCTTGTTCCAAAATACTTGCGAGAAGATGACTTAGGCAAAGAATGCAAGGAGTTGATACAAACCTTACCATTAGAAAAAGGATGGGTTAGTACCCACTTTCATCAATATCAAGGTTTTTGGATTGGCACAAGAATTCTCCAAGGTGTTTTATCTTGTCAAAAACACTTTCAAGCCCTTAATACAGATATCCTCTTAGTTACCACTCCTAAATCAGGTACCACTTGGCTCAAAGCATTAACCTTCGCTTTGATAAACCGCAAAAAATATCAAAATATTCATAATAACAATCATCCTTTGCTCACTACAAACCCTCATGTTCTTGTTCCCTTCTTGGAGGTTGATCTTTACTATGATAAAGATTATGTTCCTAACCTAAATTCATTATCCTCTCCAAGACTCTTTGCAACACACATTCCATATGATTTGTTGCCAAAATCTGTAAAAGAGTCAAGTTGTAAAGTGGTTTATCTTTGTAGAGATCCTAAAGACACTTTTGCTTCACTATGGCATTTCTCAAACAAGGTAAGATCACAAAGTAGTGGAACACTTCCATTAGAAGAGTCTTTTGATAAATTTACTAGAGGGGTCACTTTGTTTGGACCCTGTTGGGATCATGTATTAGGATATTGGAAAGAAAGCTTGGAAAGATCAAACAAGGTTATGTTTTTGAGATTTGAAGAAATGAAAATGAAGCCTTATTTTCATTTGAAAAAGCTTGCTGAATTTTTGGAGTGTCCATTCACCAAAGATGAAGAATCTAAAGGGGTGGTTGATGATATATTAAATTTGTGTAGTTTTGAGAAGCTGAGCAACTTGGAGGTTAATAAGACTGGGAAAGTGTCATTTGGTGTTGAAAACAAAGCTTATTTTCGTTGTGGCCAAGTTGGAGATGGAGAAAAGCTTCTTACAACTCAAATGATTGAGCAGTTAAACAGTGTAATTGAAGAGAAATTGGTTAAACATGGGTTTAATTTTTAG

Protein sequence

>MS.gene018902.t1

MAKLGDENSLVPKYLREDDLGKECKELIQTLPLEKGWVSTHFHQYQGFWIGTRILQGVLSCQKHFQALNTDILLVTTPKSGTTWLKALTFALINRKKYQNIHNNNHPLLTTNPHVLVPFLEVDLYYDKDYVPNLNSLSSPRLFATHIPYDLLPKSVKESSCKVVYLCRDPKDTFASLWHFSNKVRSQSSGTLPLEESFDKFTRGVTLFGPCWDHVLGYWKESLERSNKVMFLRFEEMKMKPYFHLKKLAEFLECPFTKDEESKGVVDDILNLCSFEKLSNLEVNKTGKVSFGVENKAYFRCGQVGDGEKLLTTQMIEQLNSVIEEKLVKHGFNF