Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019020.t1 | XP_024633839.1 | 95.9 | 490 | 20 | 0 | 1 | 490 | 1 | 490 | 4.70E-257 | 896.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019020.t1 | O48791 | 71.2 | 490 | 137 | 2 | 1 | 486 | 1 | 490 | 2.6e-187 | 656.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019020.t1 | A0A396IPJ6 | 95.9 | 490 | 20 | 0 | 1 | 490 | 1 | 490 | 3.4e-257 | 896.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene58836 | MS.gene019020 | PPI |
| MS.gene019020 | MS.gene38829 | PPI |
| MS.gene017694 | MS.gene019020 | PPI |
| MS.gene019020 | MS.gene013971 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019020.t1 | MTR_3g045180 | 93.976 | 498 | 22 | 1 | 1 | 490 | 1 | 498 | 0.0 | 958 |
| MS.gene019020.t1 | MTR_5g088860 | 83.910 | 491 | 75 | 2 | 1 | 487 | 1 | 491 | 0.0 | 850 |
| MS.gene019020.t1 | MTR_5g088860 | 83.910 | 491 | 75 | 2 | 1 | 487 | 23 | 513 | 0.0 | 849 |
| MS.gene019020.t1 | MTR_7g089090 | 58.402 | 488 | 197 | 3 | 1 | 483 | 1 | 487 | 0.0 | 576 |
| MS.gene019020.t1 | MTR_6g079040 | 64.228 | 123 | 42 | 2 | 86 | 207 | 41 | 162 | 3.23e-38 | 138 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019020.t1 | AT2G26770 | 73.887 | 494 | 121 | 3 | 1 | 488 | 1 | 492 | 0.0 | 753 |
| MS.gene019020.t1 | AT2G26770 | 73.887 | 494 | 121 | 3 | 1 | 488 | 1 | 492 | 0.0 | 753 |
| MS.gene019020.t1 | AT2G26770 | 73.887 | 494 | 121 | 3 | 1 | 488 | 1 | 492 | 0.0 | 753 |
| MS.gene019020.t1 | AT2G40820 | 54.352 | 471 | 208 | 3 | 19 | 483 | 47 | 516 | 0.0 | 523 |
| MS.gene019020.t1 | AT2G40820 | 54.352 | 471 | 208 | 3 | 19 | 483 | 22 | 491 | 0.0 | 523 |
| MS.gene019020.t1 | AT2G40820 | 54.352 | 471 | 208 | 3 | 19 | 483 | 22 | 491 | 0.0 | 523 |
| MS.gene019020.t1 | AT2G40820 | 54.352 | 471 | 208 | 3 | 19 | 483 | 34 | 503 | 0.0 | 522 |
| MS.gene019020.t1 | AT3G56480 | 55.794 | 466 | 198 | 4 | 25 | 483 | 25 | 489 | 1.50e-180 | 515 |
| MS.gene019020.t1 | AT2G40820 | 53.403 | 382 | 175 | 2 | 104 | 483 | 1 | 381 | 2.37e-147 | 427 |
Find 96 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGATTTCCTACTTACAAAAT+TGG | 0.176588 | 3.2:+32055119 | MS.gene019020:CDS |
| CTGCGGCGCAGGCACTATTT+TGG | 0.185288 | 3.2:+32062059 | MS.gene019020:CDS |
| TCCTTCATCGATAGCACTTT+TGG | 0.237609 | 3.2:-32055167 | None:intergenic |
| CCTCATTTATATGAATTAGA+AGG | 0.272590 | 3.2:+32059177 | MS.gene019020:CDS |
| GATGGGTTGGAATCCTTAAA+AGG | 0.280491 | 3.2:+32055870 | MS.gene019020:CDS |
| GATGATGTGAATCATTTGTC+TGG | 0.288215 | 3.2:-32061734 | None:intergenic |
| CGTCCAACGTCAAAAGGTTC+TGG | 0.297621 | 3.2:-32061103 | None:intergenic |
| TGAACTCGAATAGAATTCTT+TGG | 0.297696 | 3.2:-32061829 | None:intergenic |
| TGTACACGAAGCTATTTCTT+TGG | 0.342988 | 3.2:+32055920 | MS.gene019020:CDS |
| ACAAGGATGACCCGCAGTTT+TGG | 0.345696 | 3.2:+32055038 | MS.gene019020:CDS |
| ATTGAACATTCTGAAAGTTC+GGG | 0.352004 | 3.2:-32059252 | None:intergenic |
| AATTGAACATTCTGAAAGTT+CGG | 0.353771 | 3.2:-32059253 | None:intergenic |
| AACACGAGCTTCAAGCATTA+AGG | 0.356617 | 3.2:+32058910 | MS.gene019020:CDS |
| GCGAGTGGCGGGAAGAAATA+AGG | 0.360346 | 3.2:+32055893 | MS.gene019020:CDS |
| CCACTCTTTGAACTGCTGCC+CGG | 0.360438 | 3.2:-32057471 | None:intergenic |
| TTGCAGTTCAGTTAACTCAT+AGG | 0.368181 | 3.2:+32056741 | MS.gene019020:CDS |
| GAGAGAATGTCTCAAACTTC+AGG | 0.371204 | 3.2:+32057514 | MS.gene019020:CDS |
| TTCTGGGGCATAAACTGATT+TGG | 0.372582 | 3.2:-32061086 | None:intergenic |
| ATCACCCGAAAATGCCAAAA+AGG | 0.374989 | 3.2:+32059290 | MS.gene019020:CDS |
| CACTCTTTGAACTGCTGCCC+GGG | 0.388647 | 3.2:-32057470 | None:intergenic |
| GTAGCCAAACCTGGTTCAAT+TGG | 0.395153 | 3.2:-32061184 | None:intergenic |
| TCCCACATTAAGTATATGAA+TGG | 0.411218 | 3.2:-32061766 | None:intergenic |
| CTTTGTGGAGTTCGAGGAGG+TGG | 0.411973 | 3.2:+32062031 | MS.gene019020:CDS |
| CTTCAGCTTTGTGGAGTTCG+AGG | 0.431632 | 3.2:+32062025 | MS.gene019020:intron |
| AGGTGGGAATGCTGCGGCGC+AGG | 0.440198 | 3.2:+32062048 | MS.gene019020:CDS |
| ACAAAAGAGAGTCCTTGCTT+TGG | 0.450797 | 3.2:-32062085 | None:intergenic |
| TCCTTAAAAGGGCGAGTGGC+GGG | 0.450878 | 3.2:+32055882 | MS.gene019020:CDS |
| TGCTAAGAAACTTGTATATG+AGG | 0.457477 | 3.2:+32057414 | MS.gene019020:CDS |
| CATTTGAATCAGAGAGAGAA+AGG | 0.460633 | 3.2:+32062113 | MS.gene019020:CDS |
| AAAGAAGATCAGAGATGGGT+TGG | 0.465534 | 3.2:+32055857 | MS.gene019020:CDS |
| TGAGAATGAAGCTTTGTAAA+GGG | 0.466239 | 3.2:+32061793 | MS.gene019020:CDS |
| AGTATATGAATGGATTCAGT+TGG | 0.467239 | 3.2:-32061756 | None:intergenic |
| GTCCAACGTCAAAAGGTTCT+GGG | 0.473124 | 3.2:-32061102 | None:intergenic |
| TTAGAAGGGACTGAAACATT+AGG | 0.479469 | 3.2:+32059192 | MS.gene019020:CDS |
| GATTCATTTGTAGGCTTCTG+AGG | 0.480823 | 3.2:+32057390 | MS.gene019020:intron |
| TGCAGTTCAGTTAACTCATA+GGG | 0.484553 | 3.2:+32056742 | MS.gene019020:CDS |
| ATGAGAATGAAGCTTTGTAA+AGG | 0.488741 | 3.2:+32061792 | MS.gene019020:CDS |
| TAGTAAATTTGAGAAGGGTT+TGG | 0.490035 | 3.2:+32055256 | MS.gene019020:CDS |
| ATCCATTCATATACTTAATG+TGG | 0.494470 | 3.2:+32061764 | MS.gene019020:CDS |
| CTCATTTATATGAATTAGAA+GGG | 0.497583 | 3.2:+32059178 | MS.gene019020:CDS |
| TGGATCTAATAATCAGATCA+TGG | 0.501525 | 3.2:+32055139 | MS.gene019020:CDS |
| TAATTGCAGATTGTTCTAGC+TGG | 0.505097 | 3.2:+32062295 | MS.gene019020:intron |
| CAAGTTTGTATTATTTCAGA+AGG | 0.506009 | 3.2:+32061133 | MS.gene019020:CDS |
| ATTCTATTCGAGTTCAATGC+AGG | 0.509177 | 3.2:+32061836 | MS.gene019020:CDS |
| AGCAGTTCAAAGAGTGGAGG+AGG | 0.517740 | 3.2:+32057477 | MS.gene019020:CDS |
| CAAGTCCATCATGTTTCTGA+AGG | 0.520285 | 3.2:-32062334 | None:intergenic |
| AATATGCGTCCAACGTCAAA+AGG | 0.523688 | 3.2:-32061109 | None:intergenic |
| CCCAAAAGTGCTATCGATGA+AGG | 0.526304 | 3.2:+32055166 | MS.gene019020:CDS |
| CTTGCTAGTAAATTTGAGAA+GGG | 0.528068 | 3.2:+32055251 | MS.gene019020:CDS |
| TTTCAGAATGTTCAATTCAG+TGG | 0.532345 | 3.2:+32059259 | MS.gene019020:CDS |
| GTCTCAAACTTCAGGAAACC+AGG | 0.541401 | 3.2:+32057522 | MS.gene019020:CDS |
| CCTTGCTAGTAAATTTGAGA+AGG | 0.543952 | 3.2:+32055250 | MS.gene019020:CDS |
| AAACTCCTTCAGAAACATGA+TGG | 0.544547 | 3.2:+32062329 | MS.gene019020:CDS |
| GGACTTGGAACAATTAATGA+AGG | 0.548634 | 3.2:+32058253 | MS.gene019020:intron |
| CCTTCTCAAATTTACTAGCA+AGG | 0.554709 | 3.2:-32055250 | None:intergenic |
| TTCAGTTAACTCATAGGGAA+GGG | 0.556887 | 3.2:+32056747 | MS.gene019020:CDS |
| ATGGGTTGGAATCCTTAAAA+GGG | 0.558486 | 3.2:+32055871 | MS.gene019020:CDS |
| CAGTTGGCAAGGGAAAAGAA+AGG | 0.562244 | 3.2:+32059141 | MS.gene019020:intron |
| TGCTGCTAAGTTGTCTGAAG+AGG | 0.562336 | 3.2:+32055283 | MS.gene019020:CDS |
| TCCATTCATATACTTAATGT+GGG | 0.569079 | 3.2:+32061765 | MS.gene019020:CDS |
| CAAACCTGGTTCAATTGGAC+CGG | 0.569962 | 3.2:-32061179 | None:intergenic |
| ACGCGAAACTGCACAGCTGT+TGG | 0.571348 | 3.2:+32055199 | MS.gene019020:CDS |
| ACCTGGTTCAATTGGACCGG+TGG | 0.575977 | 3.2:-32061176 | None:intergenic |
| CCTTCTAATTCATATAAATG+AGG | 0.579496 | 3.2:-32059177 | None:intergenic |
| GGCAGCAGTTCAAAGAGTGG+AGG | 0.581141 | 3.2:+32057474 | MS.gene019020:CDS |
| CATAGTGTCACCAAAACTGC+GGG | 0.582763 | 3.2:-32055048 | None:intergenic |
| ATTAATGAAGGAGGTTCAAG+AGG | 0.588663 | 3.2:+32058265 | MS.gene019020:CDS |
| GTTCAGTTAACTCATAGGGA+AGG | 0.590453 | 3.2:+32056746 | MS.gene019020:CDS |
| TCAGTTAACTCATAGGGAAG+GGG | 0.591057 | 3.2:+32056748 | MS.gene019020:CDS |
| ATGATGAGAGATACCTTGCT+TGG | 0.592908 | 3.2:-32058314 | None:intergenic |
| ATCCTTAAAAGGGCGAGTGG+CGG | 0.594151 | 3.2:+32055881 | MS.gene019020:CDS |
| ATGATGTGAATCATTTGTCT+GGG | 0.597530 | 3.2:-32061733 | None:intergenic |
| GGCAAGGGAAAAGAAAGGAG+AGG | 0.600428 | 3.2:+32059146 | MS.gene019020:CDS |
| CAGCTTTGTGGAGTTCGAGG+AGG | 0.602632 | 3.2:+32062028 | MS.gene019020:intron |
| ATGCAGCTATCATGCTTGCA+AGG | 0.603055 | 3.2:+32062137 | MS.gene019020:CDS |
| TTTGTGGAGTTCGAGGAGGT+GGG | 0.607486 | 3.2:+32062032 | MS.gene019020:CDS |
| CTTGGAACAATTAATGAAGG+AGG | 0.607672 | 3.2:+32058256 | MS.gene019020:CDS |
| ACCACCGGTCCAATTGAACC+AGG | 0.610618 | 3.2:+32061175 | MS.gene019020:CDS |
| TGACACTATGATGATGCATA+AGG | 0.614210 | 3.2:+32055061 | MS.gene019020:CDS |
| AGGGGAGCTAGTACAACAGA+AGG | 0.616255 | 3.2:+32056766 | MS.gene019020:CDS |
| CAGATCACACTGTCAACCAC+CGG | 0.621680 | 3.2:+32061160 | MS.gene019020:CDS |
| ATGAGTTAACTGAACTGCAA+GGG | 0.627954 | 3.2:-32056738 | None:intergenic |
| GCTAGTACAACAGAAGGCTG+AGG | 0.635371 | 3.2:+32056772 | MS.gene019020:CDS |
| TTGAACATTCTGAAAGTTCG+GGG | 0.637142 | 3.2:-32059251 | None:intergenic |
| TATGAGTTAACTGAACTGCA+AGG | 0.638190 | 3.2:-32056739 | None:intergenic |
| AATGCTGCATCAGCCAAGCA+AGG | 0.639243 | 3.2:+32058301 | MS.gene019020:CDS |
| TCGAGGAGGTGGGAATGCTG+CGG | 0.640974 | 3.2:+32062042 | MS.gene019020:CDS |
| CCGGGCAGCAGTTCAAAGAG+TGG | 0.642685 | 3.2:+32057471 | MS.gene019020:CDS |
| TCCAACGTCAAAAGGTTCTG+GGG | 0.650573 | 3.2:-32061101 | None:intergenic |
| GGAATCCTTAAAAGGGCGAG+TGG | 0.655039 | 3.2:+32055878 | MS.gene019020:CDS |
| TGAACATTCTGAAAGTTCGG+GGG | 0.661686 | 3.2:-32059250 | None:intergenic |
| CAGCTATCATGCTTGCAAGG+AGG | 0.667212 | 3.2:+32062140 | MS.gene019020:CDS |
| TATGATGATGCATAAGGACG+TGG | 0.671817 | 3.2:+32055067 | MS.gene019020:CDS |
| AAAAGTGCTATCGATGAAGG+AGG | 0.674422 | 3.2:+32055169 | MS.gene019020:CDS |
| TGGTTTGGGAAACTATGTAG+AGG | 0.678930 | 3.2:+32061373 | MS.gene019020:CDS |
| TCATAGTGTCACCAAAACTG+CGG | 0.680285 | 3.2:-32055049 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 32055035 | 32062348 | 32055035 | ID=MS.gene019020 |
| chr3.2 | mRNA | 32055035 | 32062348 | 32055035 | ID=MS.gene019020.t1;Parent=MS.gene019020 |
| chr3.2 | exon | 32055035 | 32055304 | 32055035 | ID=MS.gene019020.t1.exon1;Parent=MS.gene019020.t1 |
| chr3.2 | CDS | 32055035 | 32055304 | 32055035 | ID=cds.MS.gene019020.t1;Parent=MS.gene019020.t1 |
| chr3.2 | exon | 32055813 | 32055941 | 32055813 | ID=MS.gene019020.t1.exon2;Parent=MS.gene019020.t1 |
| chr3.2 | CDS | 32055813 | 32055941 | 32055813 | ID=cds.MS.gene019020.t1;Parent=MS.gene019020.t1 |
| chr3.2 | exon | 32056731 | 32056823 | 32056731 | ID=MS.gene019020.t1.exon3;Parent=MS.gene019020.t1 |
| chr3.2 | CDS | 32056731 | 32056823 | 32056731 | ID=cds.MS.gene019020.t1;Parent=MS.gene019020.t1 |
| chr3.2 | exon | 32057403 | 32057543 | 32057403 | ID=MS.gene019020.t1.exon4;Parent=MS.gene019020.t1 |
| chr3.2 | CDS | 32057403 | 32057543 | 32057403 | ID=cds.MS.gene019020.t1;Parent=MS.gene019020.t1 |
| chr3.2 | exon | 32058254 | 32058322 | 32058254 | ID=MS.gene019020.t1.exon5;Parent=MS.gene019020.t1 |
| chr3.2 | CDS | 32058254 | 32058322 | 32058254 | ID=cds.MS.gene019020.t1;Parent=MS.gene019020.t1 |
| chr3.2 | exon | 32058897 | 32058980 | 32058897 | ID=MS.gene019020.t1.exon6;Parent=MS.gene019020.t1 |
| chr3.2 | CDS | 32058897 | 32058980 | 32058897 | ID=cds.MS.gene019020.t1;Parent=MS.gene019020.t1 |
| chr3.2 | exon | 32059144 | 32059324 | 32059144 | ID=MS.gene019020.t1.exon7;Parent=MS.gene019020.t1 |
| chr3.2 | CDS | 32059144 | 32059324 | 32059144 | ID=cds.MS.gene019020.t1;Parent=MS.gene019020.t1 |
| chr3.2 | exon | 32061080 | 32061196 | 32061080 | ID=MS.gene019020.t1.exon8;Parent=MS.gene019020.t1 |
| chr3.2 | CDS | 32061080 | 32061196 | 32061080 | ID=cds.MS.gene019020.t1;Parent=MS.gene019020.t1 |
| chr3.2 | exon | 32061369 | 32061427 | 32061369 | ID=MS.gene019020.t1.exon9;Parent=MS.gene019020.t1 |
| chr3.2 | CDS | 32061369 | 32061427 | 32061369 | ID=cds.MS.gene019020.t1;Parent=MS.gene019020.t1 |
| chr3.2 | exon | 32061723 | 32061857 | 32061723 | ID=MS.gene019020.t1.exon10;Parent=MS.gene019020.t1 |
| chr3.2 | CDS | 32061723 | 32061857 | 32061723 | ID=cds.MS.gene019020.t1;Parent=MS.gene019020.t1 |
| chr3.2 | exon | 32062031 | 32062180 | 32062031 | ID=MS.gene019020.t1.exon11;Parent=MS.gene019020.t1 |
| chr3.2 | CDS | 32062031 | 32062180 | 32062031 | ID=cds.MS.gene019020.t1;Parent=MS.gene019020.t1 |
| chr3.2 | exon | 32062304 | 32062348 | 32062304 | ID=MS.gene019020.t1.exon12;Parent=MS.gene019020.t1 |
| chr3.2 | CDS | 32062304 | 32062348 | 32062304 | ID=cds.MS.gene019020.t1;Parent=MS.gene019020.t1 |
| Gene Sequence |
| Protein sequence |
