Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019441.t1 | XP_013444959.1 | 98.6 | 358 | 5 | 0 | 1 | 358 | 1 | 358 | 1.30E-195 | 692.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019441.t1 | A8IXB8 | 57.4 | 357 | 140 | 4 | 6 | 350 | 5 | 361 | 1.3e-103 | 377.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019441.t1 | A0A072TNB3 | 98.6 | 358 | 5 | 0 | 1 | 358 | 1 | 358 | 9.2e-196 | 692.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene005906 | MS.gene019441 | PPI |
| MS.gene34068 | MS.gene019441 | PPI |
| MS.gene99989 | MS.gene019441 | PPI |
| MS.gene09533 | MS.gene019441 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019441.t1 | MTR_8g032880 | 98.603 | 358 | 5 | 0 | 1 | 358 | 1 | 358 | 0.0 | 729 |
| MS.gene019441.t1 | MTR_1g094680 | 32.817 | 323 | 176 | 11 | 21 | 308 | 33 | 349 | 2.35e-45 | 159 |
| MS.gene019441.t1 | MTR_1g094680 | 32.817 | 323 | 176 | 11 | 21 | 308 | 82 | 398 | 4.23e-45 | 159 |
| MS.gene019441.t1 | MTR_4g058005 | 30.503 | 318 | 183 | 8 | 21 | 307 | 62 | 372 | 3.27e-43 | 154 |
| MS.gene019441.t1 | MTR_1g094680 | 35.169 | 236 | 121 | 8 | 21 | 226 | 8 | 241 | 4.84e-37 | 134 |
| MS.gene019441.t1 | MTR_1g094680 | 35.169 | 236 | 121 | 8 | 21 | 226 | 82 | 315 | 6.45e-36 | 133 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019441.t1 | AT1G01910 | 82.521 | 349 | 61 | 0 | 6 | 354 | 5 | 353 | 0.0 | 581 |
| MS.gene019441.t1 | AT1G01910 | 82.521 | 349 | 61 | 0 | 6 | 354 | 5 | 353 | 0.0 | 581 |
| MS.gene019441.t1 | AT1G01910 | 82.521 | 349 | 61 | 0 | 6 | 354 | 5 | 353 | 0.0 | 581 |
| MS.gene019441.t1 | AT1G01910 | 86.149 | 296 | 41 | 0 | 6 | 301 | 5 | 300 | 0.0 | 516 |
| MS.gene019441.t1 | AT1G01910 | 80.973 | 226 | 43 | 0 | 129 | 354 | 24 | 249 | 4.18e-136 | 387 |
| MS.gene019441.t1 | AT3G10350 | 31.988 | 322 | 179 | 10 | 21 | 308 | 88 | 403 | 1.12e-44 | 159 |
| MS.gene019441.t1 | AT3G10350 | 32.602 | 319 | 172 | 10 | 21 | 308 | 119 | 425 | 5.37e-43 | 154 |
| MS.gene019441.t1 | AT5G60730 | 32.792 | 308 | 171 | 9 | 32 | 308 | 81 | 383 | 1.12e-33 | 129 |
Find 71 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCAACGAAAACCCACTTTA+AGG | 0.170782 | 8.1:+68531654 | None:intergenic |
| AAAACCCCTACTTTGGTTAA+TGG | 0.187000 | 8.1:-68531496 | MS.gene019441:CDS |
| TACAGTTGAGCATGAGGATA+TGG | 0.246895 | 8.1:-68530901 | MS.gene019441:CDS |
| ATGTCTTTGAAGAATAAATT+TGG | 0.261769 | 8.1:-68526517 | MS.gene019441:CDS |
| ATGGGAATTGCAACAGTCTA+AGG | 0.268606 | 8.1:+68526569 | None:intergenic |
| ACTTTAAGGTTTCTTGTTCT+AGG | 0.289287 | 8.1:+68531668 | None:intergenic |
| AAAAGAGGCAGAAGAAGAAT+TGG | 0.294022 | 8.1:-68524578 | MS.gene019441:CDS |
| TTTGGCATGGGTGATGATTT+TGG | 0.298574 | 8.1:-68526004 | MS.gene019441:CDS |
| CATTCTTGCTTTAAGTAACT+TGG | 0.319005 | 8.1:+68525489 | None:intergenic |
| GAGCTAGCTGGTGCAATTCC+TGG | 0.328730 | 8.1:-68530846 | MS.gene019441:CDS |
| TGTAATGTTCCTTCTGGTAA+TGG | 0.356987 | 8.1:+68531697 | None:intergenic |
| TTCATAAAGCGAGAGGAATT+CGG | 0.357721 | 8.1:+68525687 | None:intergenic |
| GAAAATGCAGCAGAAGTATC+TGG | 0.360106 | 8.1:-68525467 | MS.gene019441:CDS |
| ATTTGGAGGTTTGTTTAGTC+AGG | 0.372718 | 8.1:-68526500 | MS.gene019441:intron |
| TCATAAAGCGAGAGGAATTC+GGG | 0.385422 | 8.1:+68525688 | None:intergenic |
| CATCATACAACATGTAAAAC+TGG | 0.392796 | 8.1:+68525443 | None:intergenic |
| CTCATAGCCTCATCAATTCC+AGG | 0.396145 | 8.1:+68530828 | None:intergenic |
| TCACAGATGACTCGCATGTT+TGG | 0.400073 | 8.1:-68526022 | MS.gene019441:intron |
| GAATTGGAGAGAGTCAAAAC+AGG | 0.400385 | 8.1:-68524562 | MS.gene019441:CDS |
| AGTACAAATTGACAGGTTAC+TGG | 0.414299 | 8.1:-68524715 | MS.gene019441:intron |
| TACTTGATCTCTACTGCATA+TGG | 0.414553 | 8.1:+68524642 | None:intergenic |
| GCTTTATGAAACAGAAAGAT+TGG | 0.417282 | 8.1:-68525674 | MS.gene019441:CDS |
| GCAATCCTTGGGAAGCTAGA+AGG | 0.429425 | 8.1:-68525974 | MS.gene019441:CDS |
| TTTGGCGAAGATGCAATCCT+TGG | 0.442246 | 8.1:-68525986 | MS.gene019441:CDS |
| TAGAACAAGAAACCTTAAAG+TGG | 0.452112 | 8.1:-68531666 | MS.gene019441:CDS |
| ACAAACCATGGCATAGAGAT+TGG | 0.454098 | 8.1:+68531468 | None:intergenic |
| GTTGGTGGGAAAGGTGGTGT+TGG | 0.481112 | 8.1:-68531637 | MS.gene019441:CDS |
| GAGAAACCATTAACCAAAGT+AGG | 0.484214 | 8.1:+68531490 | None:intergenic |
| AGAAACCATTAACCAAAGTA+GGG | 0.499369 | 8.1:+68531491 | None:intergenic |
| ACAGTTGAGCATGAGGATAT+GGG | 0.500666 | 8.1:-68530900 | MS.gene019441:CDS |
| GAGGATATGGGTAGTTCTGA+TGG | 0.502699 | 8.1:-68530888 | MS.gene019441:CDS |
| TATGGGTAGTTCTGATGGGA+TGG | 0.510623 | 8.1:-68530883 | MS.gene019441:CDS |
| TTTCTCCAATCTCTATGCCA+TGG | 0.510985 | 8.1:-68531473 | MS.gene019441:intron |
| CAAGTTATCTTCGACGATGA+AGG | 0.511170 | 8.1:-68525601 | MS.gene019441:intron |
| TCATTCCTTCTAGCTTCCCA+AGG | 0.518353 | 8.1:+68525969 | None:intergenic |
| GCTGCCACTGCTTCCGCAAG+AGG | 0.520035 | 8.1:-68525404 | MS.gene019441:intron |
| TTGGCGAAGATGCAATCCTT+GGG | 0.528089 | 8.1:-68525985 | MS.gene019441:CDS |
| AGACCCTTCTCTAAAACTGA+TGG | 0.534091 | 8.1:+68526550 | None:intergenic |
| GTATTTGATACTGCTCCCAC+TGG | 0.540064 | 8.1:-68526598 | MS.gene019441:CDS |
| TTTCAGATTGGTGCAGACAA+TGG | 0.545653 | 8.1:-68526635 | MS.gene019441:intron |
| ATTGCAACAGTCTAAGGGTA+TGG | 0.549459 | 8.1:+68526575 | None:intergenic |
| AGTCTAAGGGTATGGCCAGT+GGG | 0.561604 | 8.1:+68526583 | None:intergenic |
| TGGGAAGCTAGAAGGAATGA+AGG | 0.562365 | 8.1:-68525966 | MS.gene019441:CDS |
| ACAAAACAACTTACCTCTTG+CGG | 0.570439 | 8.1:+68525391 | None:intergenic |
| GACCCTTCTCTAAAACTGAT+GGG | 0.574242 | 8.1:+68526551 | None:intergenic |
| TCTTTGAAGAATAAATTTGG+AGG | 0.574317 | 8.1:-68526514 | MS.gene019441:CDS |
| TTTAGAGAAGGGTCTTGCAA+AGG | 0.584355 | 8.1:-68526542 | MS.gene019441:CDS |
| AATTTGTTCAATGAGCTAGC+TGG | 0.584569 | 8.1:-68530858 | MS.gene019441:CDS |
| AGAACAAGAAACCTTAAAGT+GGG | 0.586075 | 8.1:-68531665 | MS.gene019441:CDS |
| GCATCGCTAAGATTATGAGC+AGG | 0.586661 | 8.1:+68531538 | None:intergenic |
| CAGTCTAAGGGTATGGCCAG+TGG | 0.587770 | 8.1:+68526582 | None:intergenic |
| ATATCCTCATGCTCAACTGT+AGG | 0.592291 | 8.1:+68530903 | None:intergenic |
| TTTCTGTTTCATAAAGCGAG+AGG | 0.594935 | 8.1:+68525680 | None:intergenic |
| GATGACTCGCATGTTTGGCA+TGG | 0.600222 | 8.1:-68526017 | MS.gene019441:CDS |
| GCTAAGATTATGAGCAGGAT+CGG | 0.605307 | 8.1:+68531543 | None:intergenic |
| ATCGGTTGAGATAATGAGAA+CGG | 0.607262 | 8.1:+68531561 | None:intergenic |
| GGATCCTACAGTTGAGCATG+AGG | 0.607923 | 8.1:-68530907 | MS.gene019441:CDS |
| AGTCAAAACAGGGAAGAACA+AGG | 0.609550 | 8.1:-68524551 | MS.gene019441:CDS |
| AGGATATGGGTAGTTCTGAT+GGG | 0.617467 | 8.1:-68530887 | MS.gene019441:CDS |
| GCTACAACACCAGTTAAAAG+AGG | 0.619245 | 8.1:-68524593 | MS.gene019441:CDS |
| TGGGAATTGCAACAGTCTAA+GGG | 0.619370 | 8.1:+68526570 | None:intergenic |
| CTTACCTCTTGCGGAAGCAG+TGG | 0.619602 | 8.1:+68525400 | None:intergenic |
| GAAACCATTAACCAAAGTAG+GGG | 0.628988 | 8.1:+68531492 | None:intergenic |
| CTACTGCATATGGATTCATA+CGG | 0.629456 | 8.1:+68524652 | None:intergenic |
| GCGGTTAGAGAATAGAGTAA+CGG | 0.642769 | 8.1:-68524617 | MS.gene019441:CDS |
| GGGAATGCAAACACAGACAA+AGG | 0.643109 | 8.1:+68525708 | None:intergenic |
| ATGACTCGCATGTTTGGCAT+GGG | 0.644788 | 8.1:-68526016 | MS.gene019441:CDS |
| GAAGATCAACCATTACCAGA+AGG | 0.651887 | 8.1:-68531706 | MS.gene019441:CDS |
| TGCAATTCCTGGAATTGATG+AGG | 0.657803 | 8.1:-68530835 | MS.gene019441:CDS |
| AATTGGAGAGAGTCAAAACA+GGG | 0.661870 | 8.1:-68524561 | MS.gene019441:CDS |
| GCAGTAGAGATCAAGTAGAG+CGG | 0.707129 | 8.1:-68524636 | MS.gene019441:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 68524546 | 68531731 | 68524546 | ID=MS.gene019441 |
| chr8.1 | mRNA | 68524546 | 68531731 | 68524546 | ID=MS.gene019441.t1;Parent=MS.gene019441 |
| chr8.1 | exon | 68531474 | 68531731 | 68531474 | ID=MS.gene019441.t1.exon1;Parent=MS.gene019441.t1 |
| chr8.1 | CDS | 68531474 | 68531731 | 68531474 | ID=cds.MS.gene019441.t1;Parent=MS.gene019441.t1 |
| chr8.1 | exon | 68530810 | 68530934 | 68530810 | ID=MS.gene019441.t1.exon2;Parent=MS.gene019441.t1 |
| chr8.1 | CDS | 68530810 | 68530934 | 68530810 | ID=cds.MS.gene019441.t1;Parent=MS.gene019441.t1 |
| chr8.1 | exon | 68526501 | 68526651 | 68526501 | ID=MS.gene019441.t1.exon3;Parent=MS.gene019441.t1 |
| chr8.1 | CDS | 68526501 | 68526651 | 68526501 | ID=cds.MS.gene019441.t1;Parent=MS.gene019441.t1 |
| chr8.1 | exon | 68525928 | 68526038 | 68525928 | ID=MS.gene019441.t1.exon4;Parent=MS.gene019441.t1 |
| chr8.1 | CDS | 68525928 | 68526038 | 68525928 | ID=cds.MS.gene019441.t1;Parent=MS.gene019441.t1 |
| chr8.1 | exon | 68525602 | 68525740 | 68525602 | ID=MS.gene019441.t1.exon5;Parent=MS.gene019441.t1 |
| chr8.1 | CDS | 68525602 | 68525740 | 68525602 | ID=cds.MS.gene019441.t1;Parent=MS.gene019441.t1 |
| chr8.1 | exon | 68525405 | 68525520 | 68525405 | ID=MS.gene019441.t1.exon6;Parent=MS.gene019441.t1 |
| chr8.1 | CDS | 68525405 | 68525520 | 68525405 | ID=cds.MS.gene019441.t1;Parent=MS.gene019441.t1 |
| chr8.1 | exon | 68524546 | 68524722 | 68524546 | ID=MS.gene019441.t1.exon7;Parent=MS.gene019441.t1 |
| chr8.1 | CDS | 68524546 | 68524722 | 68524546 | ID=cds.MS.gene019441.t1;Parent=MS.gene019441.t1 |
| Gene Sequence |
| Protein sequence |
