AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene022037

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022037.t1 MTR_2g067830 92.383 407 31 0 32 438 1 407 0.0 768
MS.gene022037.t1 MTR_2g105300 86.352 403 33 1 32 434 1 381 0.0 705
MS.gene022037.t1 MTR_6g088760 75.182 411 97 3 1 408 1 409 0.0 612
MS.gene022037.t1 MTR_8g096990 56.720 439 180 9 1 437 1 431 3.32e-169 482
MS.gene022037.t1 MTR_5g080890 55.204 442 184 10 1 437 1 433 1.53e-158 455
MS.gene022037.t1 MTR_8g038280 54.299 442 190 8 1 437 1 435 4.27e-158 454
MS.gene022037.t1 MTR_5g044770 55.220 431 186 6 7 437 7 430 4.98e-157 451
MS.gene022037.t1 MTR_5g080960 54.525 442 187 10 1 437 1 433 1.46e-156 450
MS.gene022037.t1 MTR_8g038250 51.802 444 178 6 1 437 1 415 1.14e-148 429
MS.gene022037.t1 MTR_8g038310 52.620 439 180 11 1 437 1 413 3.68e-141 410
MS.gene022037.t1 MTR_5g038360 49.887 443 209 8 1 437 1 436 2.19e-137 401
MS.gene022037.t1 MTR_3g028120 51.781 421 195 7 22 437 21 438 6.68e-136 397
MS.gene022037.t1 MTR_6g090290 50.113 443 208 8 1 437 1 436 1.37e-135 397
MS.gene022037.t1 MTR_0024s0040 49.038 416 198 8 30 437 1 410 7.58e-122 360
MS.gene022037.t1 MTR_5g044570 43.722 446 206 9 3 438 2 412 1.14e-117 350
MS.gene022037.t1 MTR_4g109760 47.836 439 186 11 1 437 1 398 3.33e-111 333
MS.gene022037.t1 MTR_5g008070 41.148 418 229 7 21 436 23 425 4.48e-99 303
MS.gene022037.t1 MTR_0036s0050 39.667 421 231 9 27 437 28 435 1.02e-93 290
MS.gene022037.t1 MTR_0036s0110 37.168 452 259 10 1 437 1 442 4.93e-93 288
MS.gene022037.t1 MTR_2g059490 39.545 440 219 10 4 436 10 409 4.34e-92 285
MS.gene022037.t1 MTR_6g088765 62.343 239 55 2 167 404 5 209 3.62e-90 273
MS.gene022037.t1 MTR_2g059460 38.389 422 215 10 23 438 25 407 1.88e-88 275
MS.gene022037.t1 MTR_0036s0070 37.560 418 235 10 27 437 28 426 4.28e-88 275
MS.gene022037.t1 MTR_5g008150 36.215 428 219 10 22 437 27 412 8.96e-81 255
MS.gene022037.t1 MTR_5g008110 34.862 436 235 10 10 437 21 415 5.74e-79 251
MS.gene022037.t1 MTR_3g097150 32.864 426 258 11 24 437 39 448 4.05e-68 224
MS.gene022037.t1 MTR_1g060750 33.663 404 233 11 40 437 2 376 4.41e-67 219
MS.gene022037.t1 MTR_4g045857 38.832 291 144 5 9 298 9 266 5.72e-58 192
MS.gene022037.t1 MTR_5g008090 37.895 285 154 7 166 437 40 314 1.85e-51 176
MS.gene022037.t1 MTR_7g117430 29.817 436 258 16 29 435 68 484 4.13e-51 179
MS.gene022037.t1 MTR_1g039270 29.234 431 263 16 28 435 58 469 2.33e-49 174
MS.gene022037.t1 MTR_1g115210 32.995 394 236 16 51 435 112 486 5.19e-49 174
MS.gene022037.t1 MTR_2g028190 30.626 431 262 14 30 435 33 451 7.13e-44 159
MS.gene022037.t1 MTR_3g053710 30.711 394 249 12 55 435 84 466 3.40e-40 150
MS.gene022037.t1 MTR_1g111350 27.064 436 247 16 21 435 82 467 1.36e-38 145
MS.gene022037.t1 MTR_5g091130 28.010 382 226 14 87 436 190 554 8.20e-36 139
MS.gene022037.t1 MTR_4g007740 31.536 371 226 15 79 436 123 478 1.01e-35 137
MS.gene022037.t1 MTR_3g046300 30.645 372 230 13 87 437 96 460 1.73e-35 137
MS.gene022037.t1 MTR_2g070430 27.646 463 288 19 2 432 3 450 2.32e-33 130
MS.gene022037.t1 MTR_6g085010 28.767 365 214 16 88 435 156 491 1.93e-32 129
MS.gene022037.t1 MTR_2g055840 27.105 380 248 8 77 435 2 373 1.18e-31 124
MS.gene022037.t1 MTR_3g053980 29.805 359 228 12 87 435 141 485 1.82e-31 125
MS.gene022037.t1 MTR_7g080230 25.057 435 284 12 30 436 11 431 6.25e-30 121
MS.gene022037.t1 MTR_8g103880 27.344 384 247 17 54 428 88 448 8.28e-27 112
MS.gene022037.t1 MTR_3g068000 27.344 384 222 16 91 435 76 441 2.37e-26 111
MS.gene022037.t1 MTR_4g056420 26.509 381 238 15 83 436 72 437 6.55e-26 110
MS.gene022037.t1 MTR_8g094590 23.936 376 238 13 86 435 71 424 1.33e-25 108
MS.gene022037.t1 MTR_0945s0010 45.161 124 64 3 316 436 1 123 2.67e-25 101
MS.gene022037.t1 MTR_1g078240 25.547 411 257 14 56 436 39 430 2.89e-25 108
MS.gene022037.t1 MTR_7g021510 24.324 407 266 16 60 436 40 434 9.55e-25 106
MS.gene022037.t1 MTR_2g103340 45.000 120 20 1 19 138 32 105 1.34e-24 100
MS.gene022037.t1 MTR_2g103340 48.052 77 35 2 356 432 88 159 4.56e-13 68.2
MS.gene022037.t1 MTR_3g096930 24.675 385 243 14 78 437 74 436 2.87e-24 104
MS.gene022037.t1 MTR_4g008110 49.123 114 34 1 166 279 48 137 3.43e-24 97.8
MS.gene022037.t1 MTR_1g078240 25.735 408 261 15 56 436 39 431 8.84e-24 103
MS.gene022037.t1 MTR_2g063110 24.268 478 278 16 28 435 45 508 1.97e-23 102
MS.gene022037.t1 MTR_2g084550 26.494 385 230 16 85 436 74 438 1.55e-22 100
MS.gene022037.t1 MTR_7g062360 26.302 384 224 16 91 435 62 425 2.30e-22 99.4
MS.gene022037.t1 MTR_7g105850 27.681 401 218 17 87 437 89 467 2.38e-22 99.4
MS.gene022037.t1 MTR_1g061940 25.478 471 287 20 3 436 2 445 2.88e-22 99.4
MS.gene022037.t1 MTR_1g029810 24.011 379 239 15 86 437 55 411 3.33e-22 98.6
MS.gene022037.t1 MTR_1g029810 24.011 379 239 15 86 437 55 411 4.28e-22 98.6
MS.gene022037.t1 MTR_7g102750 26.702 382 230 18 86 436 79 441 8.63e-22 97.8
MS.gene022037.t1 MTR_8g094590 23.810 357 229 13 86 423 71 403 1.69e-21 96.3
MS.gene022037.t1 MTR_4g023650 23.822 382 240 13 87 437 60 421 1.46e-20 94.0
MS.gene022037.t1 MTR_1g078240 26.686 341 217 12 56 378 39 364 1.21e-19 90.5
MS.gene022037.t1 MTR_5g016260 25.806 372 230 16 87 437 87 433 1.21e-19 92.0
MS.gene022037.t1 MTR_5g016260 25.806 372 230 16 87 437 87 433 1.24e-19 91.7
MS.gene022037.t1 MTR_8g075090 27.105 380 215 18 89 437 113 461 1.25e-19 91.7
MS.gene022037.t1 MTR_4g100990 24.675 462 266 20 41 436 26 471 2.01e-19 90.9
MS.gene022037.t1 MTR_7g080230 24.419 344 231 9 112 436 28 361 3.10e-19 89.7
MS.gene022037.t1 MTR_3g065160 22.995 374 241 14 90 437 76 428 4.04e-19 89.4
MS.gene022037.t1 MTR_5g022880 26.582 395 235 18 74 435 89 461 5.90e-19 89.7
MS.gene022037.t1 MTR_8g075090 26.984 378 214 18 89 435 113 459 8.04e-19 89.0
MS.gene022037.t1 MTR_7g102750 27.857 280 164 11 86 341 79 344 2.89e-18 87.0
MS.gene022037.t1 MTR_8g094590 25.385 260 169 9 86 340 71 310 8.44e-18 84.3
MS.gene022037.t1 MTR_3g014290 25.474 369 222 15 89 436 81 417 1.27e-17 84.7
MS.gene022037.t1 MTR_4g095270 26.913 379 217 17 87 437 79 425 1.40e-17 85.5
MS.gene022037.t1 MTR_4g095270 25.668 374 228 16 87 437 79 425 1.58e-17 85.5
MS.gene022037.t1 MTR_8g039540 23.841 453 295 17 9 436 7 434 1.63e-17 84.7
MS.gene022037.t1 MTR_4g023630 22.368 380 248 11 87 437 60 421 2.07e-17 84.3
MS.gene022037.t1 MTR_1g037630 25.474 369 222 15 89 436 93 429 2.49e-17 84.0
MS.gene022037.t1 MTR_5g061690 25.549 364 214 18 95 430 108 442 4.49e-15 77.8
MS.gene022037.t1 MTR_8g075010 23.861 373 222 16 95 435 107 449 3.20e-14 75.1
MS.gene022037.t1 MTR_3g100500 25.193 389 251 17 52 422 58 424 2.56e-11 65.5
MS.gene022037.t1 MTR_5g061690 24.919 309 186 16 140 430 23 303 4.33e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022037.t1 AT5G33340 47.816 412 209 5 27 437 30 436 5.02e-127 375
MS.gene022037.t1 AT1G64830 44.712 416 215 7 27 437 25 430 7.84e-113 338
MS.gene022037.t1 AT2G35615 38.826 443 252 8 9 436 6 444 2.62e-96 296
MS.gene022037.t1 AT1G31450 38.288 444 251 7 5 436 10 442 9.39e-91 282
MS.gene022037.t1 AT2G28040 35.068 442 218 16 8 436 6 391 7.34e-72 232
MS.gene022037.t1 AT2G28040 35.198 429 213 15 17 436 10 382 1.45e-71 231
MS.gene022037.t1 AT2G28010 34.459 444 233 13 1 437 1 393 9.71e-69 224
MS.gene022037.t1 AT2G03200 33.718 433 249 11 27 437 44 460 1.41e-67 223
MS.gene022037.t1 AT2G28030 37.006 354 193 9 89 437 61 389 2.12e-64 212
MS.gene022037.t1 AT2G28220 32.143 448 244 14 1 437 1 399 3.03e-62 207
MS.gene022037.t1 AT2G28225 35.754 358 196 11 89 437 67 399 1.24e-56 192
MS.gene022037.t1 AT1G01300 32.075 371 222 14 79 436 132 485 8.01e-47 168
MS.gene022037.t1 AT2G23945 26.940 464 291 15 4 437 5 450 1.09e-45 164
MS.gene022037.t1 AT3G61820 30.645 372 230 12 79 435 125 483 6.15e-45 163
MS.gene022037.t1 AT3G20015 28.409 440 273 17 19 435 50 470 1.37e-43 159
MS.gene022037.t1 AT3G18490 27.803 446 284 16 5 435 78 500 2.64e-42 156
MS.gene022037.t1 AT5G10770 32.869 359 218 14 87 436 130 474 2.64e-41 153
MS.gene022037.t1 AT1G25510 28.348 448 264 18 21 435 60 483 2.81e-41 153
MS.gene022037.t1 AT1G79720 30.201 447 269 19 15 435 53 482 1.14e-39 149
MS.gene022037.t1 AT4G30040 27.500 440 253 17 28 430 17 427 4.43e-39 146
MS.gene022037.t1 AT5G10760 29.138 429 268 14 22 435 57 464 4.66e-39 147
MS.gene022037.t1 AT3G59080 29.457 387 229 14 80 436 161 533 1.37e-35 138
MS.gene022037.t1 AT2G42980 30.077 389 226 14 80 436 151 525 1.64e-35 138
MS.gene022037.t1 AT4G30030 29.428 367 230 14 81 435 70 419 4.48e-35 135
MS.gene022037.t1 AT3G25700 24.882 422 283 12 39 436 39 450 3.12e-33 130
MS.gene022037.t1 AT3G12700 26.870 361 250 10 87 436 104 461 7.34e-32 127
MS.gene022037.t1 AT5G36260 27.660 376 231 16 86 436 75 434 2.59e-31 125
MS.gene022037.t1 AT3G02740 27.968 379 230 17 84 436 79 440 2.86e-29 119
MS.gene022037.t1 AT3G02740 27.614 373 228 16 89 436 10 365 1.03e-28 117
MS.gene022037.t1 AT2G39710 28.418 373 220 15 91 430 67 425 3.62e-28 116
MS.gene022037.t1 AT5G45120 28.010 407 222 18 89 435 83 478 1.73e-27 114
MS.gene022037.t1 AT1G65240 27.005 374 233 15 86 436 71 427 3.40e-27 113
MS.gene022037.t1 AT1G09750 27.007 411 269 14 43 435 51 448 1.31e-26 111
MS.gene022037.t1 AT4G33490 26.260 377 233 15 86 437 57 413 1.63e-26 111
MS.gene022037.t1 AT5G22850 27.368 380 231 18 86 436 78 441 5.67e-25 107
MS.gene022037.t1 AT1G05840 25.066 379 238 14 87 436 78 439 1.50e-24 106
MS.gene022037.t1 AT3G59080 27.532 385 203 15 80 436 161 497 3.34e-24 105
MS.gene022037.t1 AT4G12920 27.411 394 236 18 56 435 30 387 4.52e-24 103
MS.gene022037.t1 AT5G02190 24.202 376 232 16 98 435 82 442 6.63e-23 100
MS.gene022037.t1 AT5G43100 25.886 367 233 14 87 436 74 418 4.20e-22 99.0
MS.gene022037.t1 AT5G43100 25.886 367 233 14 87 436 74 418 1.15e-21 98.2
MS.gene022037.t1 AT4G33490 25.902 305 202 10 78 376 46 332 8.24e-21 94.4
MS.gene022037.t1 AT3G54400 25.895 363 229 14 89 436 88 425 8.47e-21 94.4
MS.gene022037.t1 AT1G44130 24.289 387 243 18 78 437 38 401 1.21e-20 94.0
MS.gene022037.t1 AT2G36670 25.000 376 243 16 86 436 97 458 1.21e-20 94.7
MS.gene022037.t1 AT1G77480 23.117 385 252 16 78 437 56 421 1.52e-20 94.0
MS.gene022037.t1 AT1G08210 26.260 377 236 16 86 436 81 441 1.54e-20 94.4
MS.gene022037.t1 AT1G08210 26.260 377 236 17 86 436 115 475 1.58e-20 94.4
MS.gene022037.t1 AT5G10080 24.788 472 286 22 12 437 7 455 1.88e-20 94.4
MS.gene022037.t1 AT1G08210 26.260 377 236 17 86 436 129 489 1.95e-20 94.4
MS.gene022037.t1 AT1G77480 23.117 385 252 16 78 437 56 421 2.49e-20 93.6
MS.gene022037.t1 AT2G36670 25.201 373 240 16 89 436 34 392 2.84e-20 93.2
MS.gene022037.t1 AT2G36670 25.201 373 240 16 89 436 34 392 2.84e-20 93.2
MS.gene022037.t1 AT2G36670 25.201 373 240 16 89 436 105 463 3.94e-20 93.2
MS.gene022037.t1 AT1G49050 24.451 364 228 17 106 437 49 397 8.23e-20 91.3
MS.gene022037.t1 AT1G66180 23.529 374 242 12 90 437 73 428 1.62e-19 90.9
MS.gene022037.t1 AT1G49050 24.675 385 241 18 87 437 201 570 1.85e-19 91.3
MS.gene022037.t1 AT3G51350 26.596 376 222 20 89 435 102 452 1.37e-17 85.5
MS.gene022037.t1 AT3G50050 24.000 375 234 15 87 437 91 438 1.55e-17 85.5
MS.gene022037.t1 AT5G07030 25.197 381 245 16 20 385 43 398 3.18e-17 84.0
MS.gene022037.t1 AT3G52500 25.758 396 233 18 87 436 88 468 4.58e-17 83.6
MS.gene022037.t1 AT5G37540 23.056 373 245 14 90 435 81 438 6.67e-17 82.8
MS.gene022037.t1 AT2G17760 26.075 372 214 20 94 435 109 449 4.32e-16 80.9
MS.gene022037.t1 AT5G24820 27.178 287 176 14 164 435 131 399 8.51e-16 79.3
MS.gene022037.t1 AT1G08210 24.505 404 236 17 86 436 81 468 8.75e-16 79.7
MS.gene022037.t1 AT3G51340 24.590 366 226 16 94 435 108 447 1.69e-14 75.9
MS.gene022037.t1 AT3G51340 24.138 377 226 16 94 435 108 459 6.64e-14 73.9
MS.gene022037.t1 AT3G25700 24.868 189 132 4 39 222 39 222 1.15e-12 69.3
MS.gene022037.t1 AT3G42550 21.558 385 227 11 71 436 62 390 4.25e-12 68.2
MS.gene022037.t1 AT3G51340 25.606 289 168 14 94 360 108 371 7.15e-11 64.3
MS.gene022037.t1 AT3G51340 25.606 289 168 13 94 360 108 371 9.55e-11 63.5

Find 87 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTAACGGCATTGAAATATT+TGG 0.086853 7.1:+79294694 MS.gene022037:CDS
ACCCCACCCTTTAAAGTTTA+TGG 0.118599 7.1:+79293776 MS.gene022037:CDS
GACATTACTGCACACTTTAA+TGG 0.121164 7.1:+79294595 MS.gene022037:CDS
GCTCAAAATAACTTGTTAAT+TGG 0.192086 7.1:+79294724 MS.gene022037:CDS
CATAAAACCATAAACTTTAA+AGG 0.205716 7.1:-79293783 None:intergenic
GGAGAAGATGTTGTTGTTTC+GGG 0.221691 7.1:+79294277 MS.gene022037:CDS
AGTTACTGTGGGATGGTATA+GGG 0.230875 7.1:-79293609 None:intergenic
ATGAGATACTCACCTAATTC+TGG 0.255340 7.1:-79293737 None:intergenic
TCAACTTTAACCCCAGAATT+AGG 0.261888 7.1:+79293725 MS.gene022037:CDS
TCCTCTAGCAAACTTAGTTT+TGG 0.274701 7.1:+79294256 MS.gene022037:CDS
TAGTTACTGTGGGATGGTAT+AGG 0.302051 7.1:-79293610 None:intergenic
TTATTTCCTAACATTGTGAT+AGG 0.323405 7.1:+79294079 MS.gene022037:CDS
TGGAGAAGATGTTGTTGTTT+CGG 0.327653 7.1:+79294276 MS.gene022037:CDS
CTAACGGCATTGAAATATTT+GGG 0.343130 7.1:+79294695 MS.gene022037:CDS
GAGTTAGACTCACTATTAAA+AGG 0.360611 7.1:-79294235 None:intergenic
CTTCTAATGTTAGAAAATAA+TGG 0.362045 7.1:-79294348 None:intergenic
CCACATCCTATCACAATGTT+AGG 0.367847 7.1:-79294085 None:intergenic
TAAAGTTGATACAGGTTGAT+TGG 0.372320 7.1:-79293711 None:intergenic
AGGAACATTTAGTTGTTTCC+CGG 0.404204 7.1:-79294572 None:intergenic
CACATAATCCCCTCTTCAAA+TGG 0.419269 7.1:-79294655 None:intergenic
TCTGGGGTTAAAGTTGATAC+AGG 0.420499 7.1:-79293719 None:intergenic
ATACAGGTAGTAACATAGTT+TGG 0.421442 7.1:+79293807 MS.gene022037:CDS
GTCTAAGTTGGTATCATATG+TGG 0.422594 7.1:+79294483 MS.gene022037:CDS
GATGTTAAGCTAAATTCTAA+TGG 0.429512 7.1:+79294622 MS.gene022037:CDS
ATAAAACCATAAACTTTAAA+GGG 0.430698 7.1:-79293782 None:intergenic
TGTGAATATTCTATTACATA+TGG 0.432694 7.1:+79293983 MS.gene022037:CDS
AACATCTCCGCCATGGGAAC+AGG 0.435864 7.1:-79293960 None:intergenic
TGAGATACTCACCTAATTCT+GGG 0.442431 7.1:-79293736 None:intergenic
AGTAAAAGCTCGGGTGTAGT+TGG 0.446985 7.1:+79294133 MS.gene022037:CDS
GAAGAGGGGATTATGTGCTT+TGG 0.453289 7.1:+79294661 MS.gene022037:CDS
CAGAGGCTTAAACGATGATC+AGG 0.461981 7.1:-79294538 None:intergenic
AATGGTTGTTTCAACTCCTA+TGG 0.462681 7.1:+79294303 MS.gene022037:CDS
TGTTGTTGTTTCGGGTGAAA+TGG 0.469051 7.1:+79294285 MS.gene022037:CDS
GCGGTGTGCCTGAGTCAATT+AGG 0.472668 7.1:-79294435 None:intergenic
GTTTACACTCAATCAATACT+TGG 0.472879 7.1:-79294794 None:intergenic
CCTAACATTGTGATAGGATG+TGG 0.482987 7.1:+79294085 MS.gene022037:CDS
GGTATTGAGAGAGAATTGTT+TGG 0.484528 7.1:-79293690 None:intergenic
GTTACTGTGGGATGGTATAG+GGG 0.486491 7.1:-79293608 None:intergenic
TTTACATGTACTAGAAGAGC+AGG 0.487766 7.1:-79293915 None:intergenic
ACCATAAACTTTAAAGGGTG+GGG 0.495087 7.1:-79293777 None:intergenic
TTAACATTAGATTCCACCTC+CGG 0.495583 7.1:+79294046 MS.gene022037:CDS
AACCATAAACTTTAAAGGGT+GGG 0.495874 7.1:-79293778 None:intergenic
CTTTCTCATGCTTCGAACAA+AGG 0.497497 7.1:+79293545 MS.gene022037:CDS
ACTCCTATGGTCAAAGTCAA+TGG 0.497706 7.1:+79294316 MS.gene022037:CDS
AGCTCGGGTGTAGTTGGAAT+GGG 0.501505 7.1:+79294139 MS.gene022037:CDS
CTTTGAAATTTAGTTACTGT+GGG 0.504168 7.1:-79293620 None:intergenic
GGTGTAGTTGGAATGGGAAG+TGG 0.516254 7.1:+79294145 MS.gene022037:CDS
GAAATTTAGTTACTGTGGGA+TGG 0.519912 7.1:-79293616 None:intergenic
TTAAAGTGTGCAGTAATGTC+AGG 0.520794 7.1:-79294592 None:intergenic
AAGCTCGGGTGTAGTTGGAA+TGG 0.530978 7.1:+79294138 MS.gene022037:CDS
AGGAAATAAGACACTAGATC+CGG 0.531296 7.1:-79294065 None:intergenic
AGCCTCTGCTATAATACCAC+CGG 0.532735 7.1:+79294553 MS.gene022037:CDS
GCAAGATGACAGTAAAAGCT+CGG 0.538279 7.1:+79294123 MS.gene022037:CDS
TTTGGTTTCATATCATCTAA+CGG 0.548893 7.1:+79294679 MS.gene022037:CDS
TCCCGGTGGTATTATAGCAG+AGG 0.551173 7.1:-79294555 None:intergenic
ACATTAGAAGCATTTAGTGT+AGG 0.556682 7.1:+79294361 MS.gene022037:CDS
CAAGATGACAGTAAAAGCTC+GGG 0.557182 7.1:+79294124 MS.gene022037:CDS
TTCACAAACATCTCCGCCAT+GGG 0.564260 7.1:-79293966 None:intergenic
AAACCATAAACTTTAAAGGG+TGG 0.565416 7.1:-79293779 None:intergenic
TCTTTGAAATTTAGTTACTG+TGG 0.570276 7.1:-79293621 None:intergenic
TGACCATTGACTTTGACCAT+AGG 0.570387 7.1:-79294319 None:intergenic
TATCTCATTAGTTACTCAGT+TGG 0.570697 7.1:+79293752 MS.gene022037:CDS
CAATACTTGGTGCAATCAGT+AGG 0.571532 7.1:-79294781 None:intergenic
ACACATATCTCCTGTTCCCA+TGG 0.580578 7.1:+79293950 MS.gene022037:CDS
TGAAGGATTAAATATAGGAG+AGG 0.584753 7.1:-79293867 None:intergenic
CGGAACATCCTAATTGACTC+AGG 0.585040 7.1:+79294427 MS.gene022037:CDS
GGTGGTGATGCAAAGTCACA+AGG 0.592249 7.1:+79294004 MS.gene022037:CDS
ATGTCGCTTGTAAAACAAGT+AGG 0.593721 7.1:+79294172 MS.gene022037:CDS
TCCAAAACTAAGTTTGCTAG+AGG 0.604610 7.1:-79294257 None:intergenic
ATTCACAAACATCTCCGCCA+TGG 0.608542 7.1:-79293967 None:intergenic
AAAGAGAAAATGCTTCTACT+CGG 0.611259 7.1:+79294407 MS.gene022037:CDS
TAAGACACTAGATCCGGAGG+TGG 0.611681 7.1:-79294059 None:intergenic
TGATCAGGAGGTTCGATACG+TGG 0.613372 7.1:-79294523 None:intergenic
AAACAAGTAGGCTTGTCAGT+TGG 0.627305 7.1:+79294184 MS.gene022037:CDS
GTGGTGATGCAAAGTCACAA+GGG 0.639435 7.1:+79294005 MS.gene022037:CDS
GCCTCTGCTATAATACCACC+GGG 0.646785 7.1:+79294554 MS.gene022037:CDS
AGATTTGGCAACATAGTAAG+CGG 0.660126 7.1:-79294454 None:intergenic
AACATTTAGTTGTTTCCCGG+TGG 0.662021 7.1:-79294569 None:intergenic
GAGATACTCACCTAATTCTG+GGG 0.666705 7.1:-79293735 None:intergenic
GAATATTCTATTACATATGG+TGG 0.666894 7.1:+79293986 MS.gene022037:CDS
GGAAACAATAGAATAGAACA+TGG 0.667630 7.1:+79294382 MS.gene022037:CDS
AGGCTTAAACGATGATCAGG+AGG 0.668210 7.1:-79294535 None:intergenic
AAATAAGACACTAGATCCGG+AGG 0.679192 7.1:-79294062 None:intergenic
TGGTGATGCAAAGTCACAAG+GGG 0.681363 7.1:+79294006 MS.gene022037:CDS
CATATCTCCTGTTCCCATGG+CGG 0.685271 7.1:+79293953 MS.gene022037:CDS
TTACATGTACTAGAAGAGCA+GGG 0.689391 7.1:-79293914 None:intergenic
TGATTGAAACATGTATGACA+AGG 0.717741 7.1:-79293842 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAACCATAAACTTTAAA+GGG - chr7.1:79293785-79293804 None:intergenic 15.0%
!! AACTTAGACAAAAAAAGATT+TGG - chr7.1:79294472-79294491 None:intergenic 20.0%
!! CATAAAACCATAAACTTTAA+AGG - chr7.1:79293786-79293805 None:intergenic 20.0%
!! CTTCTAATGTTAGAAAATAA+TGG - chr7.1:79294351-79294370 None:intergenic 20.0%
!! TGTGAATATTCTATTACATA+TGG + chr7.1:79293983-79294002 MS.gene022037:CDS 20.0%
!!! AAATCTTTTTTTGTCTAAGT+TGG + chr7.1:79294471-79294490 MS.gene022037:CDS 20.0%
!!! AAGAGATAATTTCTTTTTCT+AGG - chr7.1:79294756-79294775 None:intergenic 20.0%
!!! CTTTAAAGTTTATGGTTTTA+TGG + chr7.1:79293784-79293803 MS.gene022037:CDS 20.0%
!!! GATTTTGAAGGATTAAATAT+AGG - chr7.1:79293875-79293894 None:intergenic 20.0%
! GAATATTCTATTACATATGG+TGG + chr7.1:79293986-79294005 MS.gene022037:CDS 25.0%
! GATGTTAAGCTAAATTCTAA+TGG + chr7.1:79294622-79294641 MS.gene022037:CDS 25.0%
! TTATTTCCTAACATTGTGAT+AGG + chr7.1:79294079-79294098 MS.gene022037:CDS 25.0%
!! TTGTAACTTGAAGATTTTGA+AGG - chr7.1:79293887-79293906 None:intergenic 25.0%
!! TTTGGTTTCATATCATCTAA+CGG + chr7.1:79294679-79294698 MS.gene022037:CDS 25.0%
!!! AACATTTTTTCCATTTGAAG+AGG + chr7.1:79294645-79294664 MS.gene022037:CDS 25.0%
!!! ACATTTTTTCCATTTGAAGA+GGG + chr7.1:79294646-79294665 MS.gene022037:CDS 25.0%
!!! CTTTGAAATTTAGTTACTGT+GGG - chr7.1:79293623-79293642 None:intergenic 25.0%
!!! GCTCAAAATAACTTGTTAAT+TGG + chr7.1:79294724-79294743 MS.gene022037:CDS 25.0%
!!! TCTTTGAAATTTAGTTACTG+TGG - chr7.1:79293624-79293643 None:intergenic 25.0%
AAAGAGAAAATGCTTCTACT+CGG + chr7.1:79294407-79294426 MS.gene022037:CDS 30.0%
ATACAGGTAGTAACATAGTT+TGG + chr7.1:79293807-79293826 MS.gene022037:CDS 30.0%
GAGTTAGACTCACTATTAAA+AGG - chr7.1:79294238-79294257 None:intergenic 30.0%
GGAAACAATAGAATAGAACA+TGG + chr7.1:79294382-79294401 MS.gene022037:CDS 30.0%
TATCTCATTAGTTACTCAGT+TGG + chr7.1:79293752-79293771 MS.gene022037:CDS 30.0%
TGAAGGATTAAATATAGGAG+AGG - chr7.1:79293870-79293889 None:intergenic 30.0%
TGATTGAAACATGTATGACA+AGG - chr7.1:79293845-79293864 None:intergenic 30.0%
! AAACCATAAACTTTAAAGGG+TGG - chr7.1:79293782-79293801 None:intergenic 30.0%
! AACCATAAACTTTAAAGGGT+GGG - chr7.1:79293781-79293800 None:intergenic 30.0%
! CTAACGGCATTGAAATATTT+GGG + chr7.1:79294695-79294714 MS.gene022037:CDS 30.0%
! TCTAACGGCATTGAAATATT+TGG + chr7.1:79294694-79294713 MS.gene022037:CDS 30.0%
!! ACATTAGAAGCATTTAGTGT+AGG + chr7.1:79294361-79294380 MS.gene022037:CDS 30.0%
!! TAAAGTTGATACAGGTTGAT+TGG - chr7.1:79293714-79293733 None:intergenic 30.0%
!!! CATTTTTTCCATTTGAAGAG+GGG + chr7.1:79294647-79294666 MS.gene022037:CDS 30.0%
!!! GTTTATGGTTTTATGGATAC+AGG + chr7.1:79293791-79293810 MS.gene022037:CDS 30.0%
AGATTTGGCAACATAGTAAG+CGG - chr7.1:79294457-79294476 None:intergenic 35.0%
AGGAAATAAGACACTAGATC+CGG - chr7.1:79294068-79294087 None:intergenic 35.0%
ATGAGATACTCACCTAATTC+TGG - chr7.1:79293740-79293759 None:intergenic 35.0%
GAAATTTAGTTACTGTGGGA+TGG - chr7.1:79293619-79293638 None:intergenic 35.0%
GACATTACTGCACACTTTAA+TGG + chr7.1:79294595-79294614 MS.gene022037:CDS 35.0%
TCAACTTTAACCCCAGAATT+AGG + chr7.1:79293725-79293744 MS.gene022037:CDS 35.0%
TGAGATACTCACCTAATTCT+GGG - chr7.1:79293739-79293758 None:intergenic 35.0%
TTAAAGTGTGCAGTAATGTC+AGG - chr7.1:79294595-79294614 None:intergenic 35.0%
TTAACATTAGATTCCACCTC+CGG + chr7.1:79294046-79294065 MS.gene022037:CDS 35.0%
TTACATGTACTAGAAGAGCA+GGG - chr7.1:79293917-79293936 None:intergenic 35.0%
TTTACATGTACTAGAAGAGC+AGG - chr7.1:79293918-79293937 None:intergenic 35.0%
! AATGGTTGTTTCAACTCCTA+TGG + chr7.1:79294303-79294322 MS.gene022037:CDS 35.0%
! ACCATAAACTTTAAAGGGTG+GGG - chr7.1:79293780-79293799 None:intergenic 35.0%
! AGGAACATTTAGTTGTTTCC+CGG - chr7.1:79294575-79294594 None:intergenic 35.0%
! ATGTCGCTTGTAAAACAAGT+AGG + chr7.1:79294172-79294191 MS.gene022037:CDS 35.0%
! GGTATTGAGAGAGAATTGTT+TGG - chr7.1:79293693-79293712 None:intergenic 35.0%
! TCCTCTAGCAAACTTAGTTT+TGG + chr7.1:79294256-79294275 MS.gene022037:CDS 35.0%
!! GTCTAAGTTGGTATCATATG+TGG + chr7.1:79294483-79294502 MS.gene022037:CDS 35.0%
!! TCCAAAACTAAGTTTGCTAG+AGG - chr7.1:79294260-79294279 None:intergenic 35.0%
!! TGGAGAAGATGTTGTTGTTT+CGG + chr7.1:79294276-79294295 MS.gene022037:CDS 35.0%
!!! ACTTGTTTTACAAGCGACAT+TGG - chr7.1:79294172-79294191 None:intergenic 35.0%
AAACAAGTAGGCTTGTCAGT+TGG + chr7.1:79294184-79294203 MS.gene022037:CDS 40.0%
AAATAAGACACTAGATCCGG+AGG - chr7.1:79294065-79294084 None:intergenic 40.0%
ACTCCTATGGTCAAAGTCAA+TGG + chr7.1:79294316-79294335 MS.gene022037:CDS 40.0%
AGTTACTGTGGGATGGTATA+GGG - chr7.1:79293612-79293631 None:intergenic 40.0%
CAAGATGACAGTAAAAGCTC+GGG + chr7.1:79294124-79294143 MS.gene022037:CDS 40.0%
CACATAATCCCCTCTTCAAA+TGG - chr7.1:79294658-79294677 None:intergenic 40.0%
CCACATCCTATCACAATGTT+AGG - chr7.1:79294088-79294107 None:intergenic 40.0%
CCTAACATTGTGATAGGATG+TGG + chr7.1:79294085-79294104 MS.gene022037:CDS 40.0%
CTTTCTCATGCTTCGAACAA+AGG + chr7.1:79293545-79293564 MS.gene022037:CDS 40.0%
GAGATACTCACCTAATTCTG+GGG - chr7.1:79293738-79293757 None:intergenic 40.0%
GCAAGATGACAGTAAAAGCT+CGG + chr7.1:79294123-79294142 MS.gene022037:CDS 40.0%
TAGTTACTGTGGGATGGTAT+AGG - chr7.1:79293613-79293632 None:intergenic 40.0%
! AACATTTAGTTGTTTCCCGG+TGG - chr7.1:79294572-79294591 None:intergenic 40.0%
! ATGGTATAGGGGTGATTTTG+AGG - chr7.1:79293600-79293619 None:intergenic 40.0%
!! ACCCCACCCTTTAAAGTTTA+TGG + chr7.1:79293776-79293795 MS.gene022037:CDS 40.0%
!! CAATACTTGGTGCAATCAGT+AGG - chr7.1:79294784-79294803 None:intergenic 40.0%
!! GGAGAAGATGTTGTTGTTTC+GGG + chr7.1:79294277-79294296 MS.gene022037:CDS 40.0%
!! TCTGGGGTTAAAGTTGATAC+AGG - chr7.1:79293722-79293741 None:intergenic 40.0%
!! TGACCATTGACTTTGACCAT+AGG - chr7.1:79294322-79294341 None:intergenic 40.0%
!! TGTTGTTGTTTCGGGTGAAA+TGG + chr7.1:79294285-79294304 MS.gene022037:CDS 40.0%
ACACATATCTCCTGTTCCCA+TGG + chr7.1:79293950-79293969 MS.gene022037:CDS 45.0%
AGCCTCTGCTATAATACCAC+CGG + chr7.1:79294553-79294572 MS.gene022037:CDS 45.0%
AGGCTTAAACGATGATCAGG+AGG - chr7.1:79294538-79294557 None:intergenic 45.0%
AGTAAAAGCTCGGGTGTAGT+TGG + chr7.1:79294133-79294152 MS.gene022037:CDS 45.0%
ATTCACAAACATCTCCGCCA+TGG - chr7.1:79293970-79293989 None:intergenic 45.0%
CAGAGGCTTAAACGATGATC+AGG - chr7.1:79294541-79294560 None:intergenic 45.0%
GAAGAGGGGATTATGTGCTT+TGG + chr7.1:79294661-79294680 MS.gene022037:CDS 45.0%
GTTACTGTGGGATGGTATAG+GGG - chr7.1:79293611-79293630 None:intergenic 45.0%
TTCACAAACATCTCCGCCAT+GGG - chr7.1:79293969-79293988 None:intergenic 45.0%
! CGGAACATCCTAATTGACTC+AGG + chr7.1:79294427-79294446 MS.gene022037:CDS 45.0%
! GTGGTGATGCAAAGTCACAA+GGG + chr7.1:79294005-79294024 MS.gene022037:CDS 45.0%
! TGGTGATGCAAAGTCACAAG+GGG + chr7.1:79294006-79294025 MS.gene022037:CDS 45.0%
AAGCTCGGGTGTAGTTGGAA+TGG + chr7.1:79294138-79294157 MS.gene022037:CDS 50.0%
AGCTCGGGTGTAGTTGGAAT+GGG + chr7.1:79294139-79294158 MS.gene022037:CDS 50.0%
CATATCTCCTGTTCCCATGG+CGG + chr7.1:79293953-79293972 MS.gene022037:CDS 50.0%
GCCTCTGCTATAATACCACC+GGG + chr7.1:79294554-79294573 MS.gene022037:CDS 50.0%
GGTGTAGTTGGAATGGGAAG+TGG + chr7.1:79294145-79294164 MS.gene022037:CDS 50.0%
TAAGACACTAGATCCGGAGG+TGG - chr7.1:79294062-79294081 None:intergenic 50.0%
TCCCGGTGGTATTATAGCAG+AGG - chr7.1:79294558-79294577 None:intergenic 50.0%
TGATCAGGAGGTTCGATACG+TGG - chr7.1:79294526-79294545 None:intergenic 50.0%
! GGTGGTGATGCAAAGTCACA+AGG + chr7.1:79294004-79294023 MS.gene022037:CDS 50.0%
!! GGGGTGATTTTGAGGAGTCA+CGG - chr7.1:79293592-79293611 None:intergenic 50.0%
AACATCTCCGCCATGGGAAC+AGG - chr7.1:79293963-79293982 None:intergenic 55.0%
GCGGTGTGCCTGAGTCAATT+AGG - chr7.1:79294438-79294457 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 79293488 79294804 79293488 ID=MS.gene022037
chr7.1 mRNA 79293488 79294804 79293488 ID=MS.gene022037.t1;Parent=MS.gene022037
chr7.1 exon 79293488 79294804 79293488 ID=MS.gene022037.t1.exon1;Parent=MS.gene022037.t1
chr7.1 CDS 79293488 79294804 79293488 ID=cds.MS.gene022037.t1;Parent=MS.gene022037.t1
Gene Sequence

>MS.gene022037

ATGTCTCGTTTTTCAGTTTTGACGCTTATTTTCTTTTATCTTTCTTGTTTCATTTATCTTTCTCATGCTTCGAACAAAGGTTTCAGTGTTGAAATGATTCACCGTGACTCCTCAAAATCACCCCTATACCATCCCACAGTAACTAAATTTCAAAGAGCTTATAATATTGTGCATCGTTCGATCAATCGTGTCAATTACTTCACCAAACAATTCTCTCTCAATACCAATCAACCTGTATCAACTTTAACCCCAGAATTAGGTGAGTATCTCATTAGTTACTCAGTTGGCACCCCACCCTTTAAAGTTTATGGTTTTATGGATACAGGTAGTAACATAGTTTGGCTTCAATGTCAGCCTTGTCATACATGTTTCAATCAAACCTCTCCTATATTTAATCCTTCAAAATCTTCAAGTTACAAAAATATTCCCTGCTCTTCTAGTACATGTAAAGATACAAATGAGACACATATCTCCTGTTCCCATGGCGGAGATGTTTGTGAATATTCTATTACATATGGTGGTGATGCAAAGTCACAAGGGGATCTTAGTAATGATACTTTAACATTAGATTCCACCTCCGGATCTAGTGTCTTATTTCCTAACATTGTGATAGGATGTGGACACAACAATGTGCTGCAAGATGACAGTAAAAGCTCGGGTGTAGTTGGAATGGGAAGTGGACCAATGTCGCTTGTAAAACAAGTAGGCTTGTCAGTTGGTAGCAACAAATTCTCTTACTGTTTGATTCCTTTTAATAGTGAGTCTAACTCCTCTAGCAAACTTAGTTTTGGAGAAGATGTTGTTGTTTCGGGTGAAATGGTTGTTTCAACTCCTATGGTCAAAGTCAATGGTCAAGAACACCATTATTTTCTAACATTAGAAGCATTTAGTGTAGGAAACAATAGAATAGAACATGGAGAAAGAGAAAATGCTTCTACTCGGAACATCCTAATTGACTCAGGCACACCGCTTACTATGTTGCCAAATCTTTTTTTGTCTAAGTTGGTATCATATGTGGCACAAGAGATTAAACTACCACGTATCGAACCTCCTGATCATCGTTTAAGCCTCTGCTATAATACCACCGGGAAACAACTAAATGTTCCTGACATTACTGCACACTTTAATGGTGCAGATGTTAAGCTAAATTCTAATGGAACATTTTTTCCATTTGAAGAGGGGATTATGTGCTTTGGTTTCATATCATCTAACGGCATTGAAATATTTGGGAACATAGCTCAAAATAACTTGTTAATTGGCTATGACCTAGAAAAAGAAATTATCTCTTTCAAGCCTACTGATTGCACCAAGTATTGA
Protein sequence

>MS.gene022037.t1

MSRFSVLTLIFFYLSCFIYLSHASNKGFSVEMIHRDSSKSPLYHPTVTKFQRAYNIVHRSINRVNYFTKQFSLNTNQPVSTLTPELGEYLISYSVGTPPFKVYGFMDTGSNIVWLQCQPCHTCFNQTSPIFNPSKSSSYKNIPCSSSTCKDTNETHISCSHGGDVCEYSITYGGDAKSQGDLSNDTLTLDSTSGSSVLFPNIVIGCGHNNVLQDDSKSSGVVGMGSGPMSLVKQVGLSVGSNKFSYCLIPFNSESNSSSKLSFGEDVVVSGEMVVSTPMVKVNGQEHHYFLTLEAFSVGNNRIEHGERENASTRNILIDSGTPLTMLPNLFLSKLVSYVAQEIKLPRIEPPDHRLSLCYNTTGKQLNVPDITAHFNGADVKLNSNGTFFPFEEGIMCFGFISSNGIEIFGNIAQNNLLIGYDLEKEIISFKPTDCTKY