AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene023200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023200.t1 MTR_4g094715 96.178 471 8 1 1 471 1 461 0.0 931
MS.gene023200.t1 MTR_2g032710 74.735 471 111 3 1 471 1 463 0.0 707
MS.gene023200.t1 MTR_1g086390 63.333 390 141 1 74 461 80 469 0.0 542
MS.gene023200.t1 MTR_7g110050 60.621 419 161 3 53 469 46 462 0.0 539
MS.gene023200.t1 MTR_5g034090 43.552 473 226 7 21 458 56 522 5.13e-126 377
MS.gene023200.t1 MTR_8g065010 46.604 427 194 7 58 458 91 509 3.49e-125 374
MS.gene023200.t1 MTR_6g005630 46.348 397 195 6 75 470 45 424 6.81e-122 362
MS.gene023200.t1 MTR_6g005630 43.577 397 183 8 75 470 45 401 8.57e-108 325
MS.gene023200.t1 MTR_5g033680 40.250 400 226 6 60 456 50 439 2.19e-104 319
MS.gene023200.t1 MTR_8g065030 41.117 394 219 6 74 465 64 446 4.40e-102 313
MS.gene023200.t1 MTR_1g052315 44.101 356 185 7 70 422 59 403 3.95e-94 292
MS.gene023200.t1 MTR_7g091850 44.413 358 186 6 74 428 75 422 1.35e-92 288
MS.gene023200.t1 MTR_2g103410 40.000 380 215 7 74 450 58 427 2.36e-92 288
MS.gene023200.t1 MTR_2g060350 43.251 363 191 7 69 427 68 419 6.08e-90 281
MS.gene023200.t1 MTR_7g091840 43.769 329 171 5 69 392 59 378 2.31e-86 270
MS.gene023200.t1 MTR_5g034090 41.926 353 167 5 21 339 56 404 1.61e-81 258
MS.gene023200.t1 MTR_2g103650 36.031 383 233 7 75 453 28 402 1.41e-79 253
MS.gene023200.t1 MTR_3g030540 37.963 432 236 9 7 432 10 415 1.98e-77 248
MS.gene023200.t1 MTR_3g054320 37.245 392 220 10 75 461 30 400 4.89e-76 244
MS.gene023200.t1 MTR_6g028080 36.719 384 222 9 75 453 30 397 3.16e-74 239
MS.gene023200.t1 MTR_3g084380 35.714 406 233 10 53 453 5 387 6.43e-73 236
MS.gene023200.t1 MTR_2g049400 37.371 388 224 9 68 453 21 391 1.24e-72 234
MS.gene023200.t1 MTR_3g438050 36.883 385 223 8 74 453 26 395 1.44e-72 234
MS.gene023200.t1 MTR_1g012390 36.104 385 227 9 75 455 28 397 2.13e-70 229
MS.gene023200.t1 MTR_8g010070 35.385 390 223 10 70 455 26 390 1.26e-69 227
MS.gene023200.t1 MTR_4g084050 35.342 365 209 8 75 428 23 371 6.10e-69 225
MS.gene023200.t1 MTR_5g043350 36.020 397 226 12 66 455 23 398 1.33e-68 224
MS.gene023200.t1 MTR_4g083660 37.275 389 223 13 69 453 3 374 4.56e-68 222
MS.gene023200.t1 MTR_8g010080 35.659 387 220 10 70 453 26 386 5.86e-67 220
MS.gene023200.t1 MTR_1g088160 36.387 382 215 12 68 438 28 392 1.13e-66 219
MS.gene023200.t1 MTR_8g030930 35.754 358 210 7 74 424 26 370 1.90e-66 219
MS.gene023200.t1 MTR_8g010040 35.433 381 218 9 75 452 32 387 6.06e-66 217
MS.gene023200.t1 MTR_0157s0060 35.864 382 217 8 80 455 3 362 6.72e-66 216
MS.gene023200.t1 MTR_4g034020 35.864 382 217 8 80 455 3 362 6.72e-66 216
MS.gene023200.t1 MTR_8g008765 35.671 328 186 5 70 394 26 331 4.10e-65 214
MS.gene023200.t1 MTR_3g084360 35.278 360 211 9 75 428 24 367 6.43e-65 214
MS.gene023200.t1 MTR_2g058640 35.475 358 218 8 74 428 24 371 1.17e-64 214
MS.gene023200.t1 MTR_2g058670 34.943 352 217 7 74 422 24 366 3.77e-64 213
MS.gene023200.t1 MTR_8g010110 33.592 387 229 8 70 453 26 387 8.71e-64 211
MS.gene023200.t1 MTR_2g058840 33.983 359 209 8 74 422 24 364 1.27e-62 209
MS.gene023200.t1 MTR_8g010090 32.817 387 232 9 70 453 26 387 1.80e-62 208
MS.gene023200.t1 MTR_7g113910 35.501 369 151 8 74 440 56 339 5.32e-62 206
MS.gene023200.t1 MTR_7g035415 36.508 315 176 7 74 383 39 334 6.61e-62 205
MS.gene023200.t1 MTR_2g435640 34.181 354 217 7 74 422 52 394 6.77e-62 207
MS.gene023200.t1 MTR_8g009810 35.509 383 226 11 75 453 35 400 7.50e-62 207
MS.gene023200.t1 MTR_2g103410 38.806 268 154 4 74 339 58 317 4.58e-61 202
MS.gene023200.t1 MTR_2g029170 39.506 324 175 10 107 422 23 333 5.96e-60 201
MS.gene023200.t1 MTR_5g097860 36.036 333 182 8 74 394 24 337 7.92e-60 200
MS.gene023200.t1 MTR_1g013730 31.937 382 229 9 75 453 27 380 2.74e-57 194
MS.gene023200.t1 MTR_4g120320 33.249 394 242 9 67 450 38 420 4.12e-57 195
MS.gene023200.t1 MTR_1g013740 31.510 384 232 9 75 455 35 390 1.29e-56 192
MS.gene023200.t1 MTR_8g010050 32.216 388 200 11 70 453 26 354 1.94e-56 191
MS.gene023200.t1 MTR_8g077320 30.711 394 196 10 74 452 32 363 1.43e-51 179
MS.gene023200.t1 MTR_5g063430 32.667 300 181 6 74 369 24 306 5.74e-48 167
MS.gene023200.t1 MTR_8g008700 32.817 323 170 8 133 453 13 290 9.29e-48 166
MS.gene023200.t1 MTR_2g100040 30.447 358 183 11 70 421 20 317 7.38e-41 150
MS.gene023200.t1 MTR_8g031180 37.019 208 121 3 222 423 70 273 8.18e-39 142
MS.gene023200.t1 MTR_4g124980 27.273 407 224 15 57 422 61 436 1.81e-24 106
MS.gene023200.t1 MTR_2g042740 27.415 383 227 14 73 422 54 418 1.43e-23 103
MS.gene023200.t1 MTR_1g100040 26.074 349 211 10 77 391 58 393 9.65e-19 89.0
MS.gene023200.t1 MTR_5g090160 27.532 316 188 9 77 364 46 348 2.26e-18 87.8
MS.gene023200.t1 MTR_1g086030 27.726 321 188 9 77 368 84 389 2.42e-17 84.7
MS.gene023200.t1 MTR_7g110710 27.245 323 188 10 77 368 45 351 2.84e-17 84.3
MS.gene023200.t1 MTR_3g449590 26.074 326 200 10 66 364 39 350 3.18e-17 84.3
MS.gene023200.t1 MTR_7g110710 27.329 322 189 9 77 368 45 351 3.31e-17 84.3
MS.gene023200.t1 MTR_1g100050 25.926 351 209 14 77 391 52 387 5.15e-17 83.6
MS.gene023200.t1 MTR_7g088550 26.282 312 185 10 77 358 52 348 5.40e-17 83.6
MS.gene023200.t1 MTR_3g096330 25.526 380 227 13 9 358 21 374 6.70e-17 84.0
MS.gene023200.t1 MTR_8g098840 26.498 317 178 12 77 358 46 342 7.91e-17 83.2
MS.gene023200.t1 MTR_1g100060 24.928 349 215 10 77 391 95 430 8.05e-17 83.2
MS.gene023200.t1 MTR_1g099850 25.000 352 211 12 77 391 57 392 1.68e-16 82.0
MS.gene023200.t1 MTR_8g028725 24.633 341 215 10 80 391 60 387 3.11e-15 78.2
MS.gene023200.t1 MTR_2g435340 27.492 331 185 13 65 362 31 339 7.32e-15 77.0
MS.gene023200.t1 MTR_1g086030 28.159 277 157 8 121 368 22 285 1.94e-14 75.5
MS.gene023200.t1 MTR_4g120730 25.234 321 195 9 77 367 42 347 2.01e-14 75.5
MS.gene023200.t1 MTR_7g078680 25.484 310 190 9 77 358 57 353 6.76e-14 73.9
MS.gene023200.t1 MTR_2g435340 29.353 201 120 4 173 362 85 274 6.57e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023200.t1 AT1G48100 68.898 463 139 3 1 458 1 463 0.0 657
MS.gene023200.t1 AT1G02460 61.381 391 146 3 74 459 88 478 5.26e-180 513
MS.gene023200.t1 AT1G56710 57.213 409 169 4 54 459 28 433 6.43e-170 485
MS.gene023200.t1 AT4G01890 55.583 403 173 3 63 460 53 454 8.25e-167 478
MS.gene023200.t1 AT5G14650 47.179 390 190 6 75 461 51 427 1.06e-120 360
MS.gene023200.t1 AT1G60590 43.614 415 201 6 75 462 123 531 7.14e-118 356
MS.gene023200.t1 AT1G10640 44.743 409 196 6 74 458 118 520 3.19e-116 352
MS.gene023200.t1 AT3G26610 38.925 465 256 10 1 457 7 451 4.21e-107 326
MS.gene023200.t1 AT1G80170 42.932 382 206 6 71 449 49 421 4.82e-107 325
MS.gene023200.t1 AT1G70500 41.667 396 218 6 74 467 66 450 2.23e-106 324
MS.gene023200.t1 AT1G23460 41.294 402 219 7 62 459 30 418 1.12e-100 308
MS.gene023200.t1 AT1G23460 41.294 402 219 7 62 459 54 442 1.74e-100 309
MS.gene023200.t1 AT3G57510 45.299 351 175 7 77 422 70 408 6.47e-94 291
MS.gene023200.t1 AT3G07970 39.173 411 232 9 35 441 33 429 9.47e-91 283
MS.gene023200.t1 AT2G41850 42.857 350 185 6 77 422 70 408 2.01e-88 277
MS.gene023200.t1 AT1G05660 37.887 388 223 8 70 455 23 394 6.99e-78 248
MS.gene023200.t1 AT1G05650 37.179 390 227 8 68 455 21 394 9.98e-76 243
MS.gene023200.t1 AT3G15720 38.718 390 206 12 75 454 23 389 2.96e-75 243
MS.gene023200.t1 AT3G15720 38.718 390 206 12 75 454 24 390 3.01e-75 243
MS.gene023200.t1 AT2G43890 36.979 384 224 11 67 448 21 388 1.14e-74 240
MS.gene023200.t1 AT4G35670 35.955 356 210 6 75 427 24 364 1.15e-74 240
MS.gene023200.t1 AT5G14650 44.737 266 135 3 75 339 51 305 6.28e-74 235
MS.gene023200.t1 AT2G43870 39.316 351 196 7 75 422 21 357 2.05e-73 236
MS.gene023200.t1 AT3G15720 38.974 390 202 13 75 454 23 386 7.44e-73 237
MS.gene023200.t1 AT2G43880 37.076 383 218 10 75 453 31 394 4.39e-72 233
MS.gene023200.t1 AT1G80170 42.804 271 145 4 71 339 49 311 9.16e-72 230
MS.gene023200.t1 AT5G44840 34.492 374 219 8 74 440 23 377 2.22e-71 231
MS.gene023200.t1 AT1G80140 35.143 350 209 6 107 453 2 336 2.31e-70 227
MS.gene023200.t1 AT5G27530 37.222 360 196 7 74 428 52 386 4.74e-70 230
MS.gene023200.t1 AT2G33160 37.624 404 225 11 56 452 19 402 5.29e-70 235
MS.gene023200.t1 AT3G59850 39.377 353 194 10 75 422 23 360 2.52e-69 226
MS.gene023200.t1 AT3G59850 39.377 353 194 10 75 422 67 404 6.56e-69 226
MS.gene023200.t1 AT1G02790 36.872 358 203 8 68 422 45 382 9.74e-69 225
MS.gene023200.t1 AT1G65570 37.500 384 218 12 75 453 31 397 3.04e-68 223
MS.gene023200.t1 AT2G43860 36.979 384 223 11 75 455 33 400 1.93e-67 221
MS.gene023200.t1 AT1G78400 35.733 389 224 10 74 454 34 404 6.52e-64 212
MS.gene023200.t1 AT1G17150 36.104 385 226 8 74 453 32 401 1.02e-63 212
MS.gene023200.t1 AT4G13760 36.134 357 206 9 74 422 30 372 4.19e-63 210
MS.gene023200.t1 AT1G43080 36.415 357 205 9 74 422 30 372 5.64e-63 210
MS.gene023200.t1 AT5G17200 34.063 411 221 12 71 455 35 421 1.54e-62 209
MS.gene023200.t1 AT1G43090 36.695 357 204 9 74 422 30 372 2.37e-62 209
MS.gene023200.t1 AT5G39910 31.328 399 192 10 74 454 35 369 2.85e-61 204
MS.gene023200.t1 AT3G14040 39.660 353 195 9 75 423 71 409 5.08e-61 206
MS.gene023200.t1 AT4G18180 36.413 368 203 8 68 422 32 381 6.39e-61 205
MS.gene023200.t1 AT3G15720 37.356 348 186 11 116 454 2 326 1.44e-60 203
MS.gene023200.t1 AT2G43880 38.851 296 167 5 75 369 31 313 1.90e-60 201
MS.gene023200.t1 AT5G44830 32.840 338 197 6 106 440 8 318 2.10e-60 201
MS.gene023200.t1 AT1G43100 36.134 357 206 9 74 422 30 372 3.09e-60 204
MS.gene023200.t1 AT2G26620 36.056 355 207 9 74 422 30 370 2.19e-59 200
MS.gene023200.t1 AT3G07850 39.093 353 197 9 75 423 70 408 2.58e-59 201
MS.gene023200.t1 AT2G40310 35.955 356 206 9 75 422 31 372 3.29e-59 200
MS.gene023200.t1 AT3G07830 35.503 338 202 7 88 422 34 358 7.28e-59 199
MS.gene023200.t1 AT3G07820 36.337 344 202 8 83 422 27 357 7.48e-59 199
MS.gene023200.t1 AT3G07840 34.971 346 209 7 80 422 26 358 1.05e-58 199
MS.gene023200.t1 AT4G32380 32.976 373 222 8 89 452 41 394 1.18e-58 200
MS.gene023200.t1 AT2G15460 35.475 358 207 9 74 422 30 372 1.41e-58 198
MS.gene023200.t1 AT5G48140 34.911 338 203 7 88 422 34 357 1.59e-58 198
MS.gene023200.t1 AT2G15450 35.196 358 208 9 74 422 30 372 3.48e-58 197
MS.gene023200.t1 AT2G15470 34.916 358 209 9 74 422 30 372 1.30e-56 193
MS.gene023200.t1 AT4G35670 35.185 270 161 3 158 427 2 257 4.08e-53 180
MS.gene023200.t1 AT4G32370 36.860 293 163 6 89 373 49 327 1.26e-52 181
MS.gene023200.t1 AT4G32375 36.667 270 149 4 158 422 13 265 1.82e-50 177
MS.gene023200.t1 AT4G32380 29.459 370 198 8 89 452 41 353 5.96e-46 165
MS.gene023200.t1 AT4G33440 28.363 342 191 12 48 358 52 370 3.00e-23 102
MS.gene023200.t1 AT3G42950 26.842 380 233 12 73 422 64 428 7.44e-21 95.5
MS.gene023200.t1 AT3G62110 26.437 348 210 11 77 393 45 377 6.01e-20 92.8
MS.gene023200.t1 AT3G62110 26.437 348 210 11 77 393 45 377 6.01e-20 92.8
MS.gene023200.t1 AT1G19170 24.627 402 235 14 63 422 75 450 9.43e-20 92.4
MS.gene023200.t1 AT5G41870 26.316 323 189 11 74 364 44 349 4.52e-19 89.7
MS.gene023200.t1 AT5G49215 25.600 375 235 11 77 422 43 402 1.24e-17 85.5
MS.gene023200.t1 AT5G49215 25.600 375 235 11 77 422 45 404 1.26e-17 85.5
MS.gene023200.t1 AT4G23820 25.868 317 191 8 77 364 43 344 1.92e-17 84.7
MS.gene023200.t1 AT3G48950 23.962 313 192 8 77 358 47 344 5.65e-17 83.6
MS.gene023200.t1 AT2G23900 25.641 312 188 10 77 358 58 355 5.22e-16 80.5
MS.gene023200.t1 AT3G61490 24.839 310 192 10 77 358 62 358 1.09e-14 76.6
MS.gene023200.t1 AT3G61490 24.839 310 192 10 77 358 160 456 1.27e-14 76.6
MS.gene023200.t1 AT3G61490 24.839 310 192 10 77 358 47 343 1.34e-14 76.3
MS.gene023200.t1 AT3G61490 24.839 310 192 10 77 358 47 343 1.34e-14 76.3
MS.gene023200.t1 AT3G61490 24.839 310 192 10 77 358 47 343 1.34e-14 76.3
MS.gene023200.t1 AT4G23500 26.537 309 176 11 80 355 73 363 3.30e-13 72.0

Find 108 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCTCCAATTAATTGTTTA+AGG 0.064758 4.3:-18402289 MS.gene023200:CDS
CAGTTCCACATGATATTTAA+TGG 0.196238 4.3:-18403325 MS.gene023200:CDS
CCTTCCCTTAAACAATTAAT+TGG 0.200232 4.3:+18402284 None:intergenic
TTCATAGACTATTTATTCTT+TGG 0.204045 4.3:-18404891 MS.gene023200:CDS
TTAATTACCATTGCTTATTA+AGG 0.227433 4.3:+18403195 None:intergenic
CCATAAGCATTCCAACAAAA+AGG 0.243640 4.3:+18402332 None:intergenic
GTTTACCCTGTGAGGTTTGC+AGG 0.305698 4.3:+18404144 None:intergenic
GACGAATCCCGAGGATCATT+TGG 0.309383 4.3:+18404715 None:intergenic
GTTACATCATTTGGAGCAAT+TGG 0.311153 4.3:-18404673 MS.gene023200:CDS
TAGTCCATTCACATACAAAT+TGG 0.324642 4.3:+18403366 None:intergenic
AACAATAGTATTGGTCTATA+AGG 0.326820 4.3:+18402543 None:intergenic
TGTAGAGTCAGGAGTTCTTC+TGG 0.355959 4.3:-18404593 MS.gene023200:CDS
TTGATCTCCATTTCCTTCAA+TGG 0.361661 4.3:+18404180 None:intergenic
GAAAGATCACCCACAGTCTC+AGG 0.377658 4.3:+18402257 None:intergenic
GGATCATTTGAAGACTTAGA+AGG 0.381774 4.3:+18404784 None:intergenic
GGTGATGATTGCATTTCAAT+TGG 0.384128 4.3:-18403051 MS.gene023200:CDS
ACATGGATTCCATCAGTGTT+GGG 0.385395 4.3:+18403250 None:intergenic
CTGTACCATTAAAACTCATT+TGG 0.389055 4.3:+18404206 None:intergenic
ACCTCCAATTAATTGTTTAA+GGG 0.389356 4.3:-18402288 MS.gene023200:CDS
TCTGCCTCTGGCCATGAATC+AGG 0.393547 4.3:+18404265 None:intergenic
TGATCCTCGGGATTCGTCTT+CGG 0.398088 4.3:-18404710 MS.gene023200:CDS
TCAGTGTTGGGGCTAAGTTT+TGG 0.401262 4.3:+18403262 None:intergenic
GGCCATGAATCAGGTCCATC+TGG 0.406021 4.3:+18404274 None:intergenic
GTGGTCCTAGCCACGGGATT+AGG 0.416076 4.3:-18402990 MS.gene023200:intron
TTCCATGCTTTCTTGAATGC+AGG 0.417079 4.3:+18404628 None:intergenic
TCACTACAAGCAAAATGAAT+AGG 0.422913 4.3:+18402428 None:intergenic
TGATTTCTGCTATCTGCCTC+TGG 0.424057 4.3:+18404253 None:intergenic
CTTACTTGAAATACTAATCC+TGG 0.430828 4.3:+18404514 None:intergenic
ACAGGAACCATTGAAGGAAA+TGG 0.436139 4.3:-18404187 MS.gene023200:CDS
CCTTTGTCAAACAATAGTAT+TGG 0.440502 4.3:+18402534 None:intergenic
CACCAGATGGACCTGATTCA+TGG 0.440641 4.3:-18404276 MS.gene023200:CDS
AGGAACACTAGGAGAAGAGT+TGG 0.444516 4.3:+18404750 None:intergenic
AACATGGATTCCATCAGTGT+TGG 0.447865 4.3:+18403249 None:intergenic
TCCCGAGGATCATTTGGATA+AGG 0.448420 4.3:+18404721 None:intergenic
AATGGTACAGGAACCATTGA+AGG 0.452512 4.3:-18404193 MS.gene023200:CDS
CCCTTATCCAAATGATCCTC+GGG 0.453352 4.3:-18404722 MS.gene023200:CDS
GCAGCTTGTGCTGTAGAGTC+AGG 0.454931 4.3:-18404604 MS.gene023200:CDS
ATCTTCCCTGCAAACCTCAC+AGG 0.456078 4.3:-18404150 MS.gene023200:CDS
AAAATTTGAGAACATACAAA+TGG 0.456117 4.3:-18402585 MS.gene023200:CDS
TCAGGTGGATGGTATGCTAA+TGG 0.461251 4.3:-18404299 MS.gene023200:intron
GCCATTTGAAGGTGAATTGT+TGG 0.464227 4.3:-18402360 MS.gene023200:CDS
TCCCTTAAACAATTAATTGG+AGG 0.466396 4.3:+18402287 None:intergenic
TTTCATATCTATAGATGATA+CGG 0.467936 4.3:-18403402 MS.gene023200:intron
AAGACATGGCAAGGTGGAAT+GGG 0.468398 4.3:-18402623 MS.gene023200:CDS
TATTTCCACATTTGAAGTCC+CGG 0.469866 4.3:+18403026 None:intergenic
CCCGAGGATCATTTGGATAA+GGG 0.470657 4.3:+18404722 None:intergenic
GTTGAAAACTCACAAGATGT+TGG 0.482732 4.3:-18403229 MS.gene023200:CDS
TAAGTTTGAGACACATGCTT+GGG 0.483234 4.3:+18402694 None:intergenic
TTGAAAACTCACAAGATGTT+GGG 0.483533 4.3:-18403228 MS.gene023200:CDS
TTGAAATTTAATCAGGTGGA+TGG 0.487001 4.3:-18404310 MS.gene023200:intron
AGCTCCAATTTGTATGTGAA+TGG 0.494817 4.3:-18403370 MS.gene023200:CDS
CCAATTAATTGTTTAAGGGA+AGG 0.495839 4.3:-18402284 MS.gene023200:CDS
TTGTACAGCATAGGAAGTCT+TGG 0.499986 4.3:-18402728 MS.gene023200:intron
ACTACTTAATTTGAGAAATG+TGG 0.500752 4.3:-18404857 MS.gene023200:CDS
AAAGACATGGCAAGGTGGAA+TGG 0.501829 4.3:-18402624 MS.gene023200:CDS
ACCCTTATCCAAATGATCCT+CGG 0.501972 4.3:-18404723 MS.gene023200:CDS
TCCAACAATTCACCTTCAAA+TGG 0.505935 4.3:+18402359 None:intergenic
AATACTAATCCTGGTTTGCA+TGG 0.507411 4.3:+18404523 None:intergenic
ACATATTGATCATTACTCCT+TGG 0.515439 4.3:+18403299 None:intergenic
TAACTTGTGGTCCTAGCCAC+GGG 0.522097 4.3:-18402996 MS.gene023200:CDS
ACTTCAAATGTGGAAATAGC+TGG 0.529833 4.3:-18403021 MS.gene023200:CDS
TCATTTGGAGCAATTGGAGA+TGG 0.537985 4.3:-18404667 MS.gene023200:CDS
GTAACTTGTGGTCCTAGCCA+CGG 0.538941 4.3:-18402997 MS.gene023200:CDS
ATTTGAGAAATGTGGATGGA+AGG 0.539093 4.3:-18404849 MS.gene023200:CDS
ATGATATTTAATGGCTGCCA+AGG 0.542871 4.3:-18403316 MS.gene023200:CDS
AACATACAAATGGAGAATGT+AGG 0.544153 4.3:-18402575 MS.gene023200:CDS
ATTTCCACATTTGAAGTCCC+GGG 0.546166 4.3:+18403027 None:intergenic
AAGGAAATGGAGATCAATGG+TGG 0.551216 4.3:-18404174 MS.gene023200:CDS
TTAAGTTTGAGACACATGCT+TGG 0.553610 4.3:+18402693 None:intergenic
AAACTTAGCCCCAACACTGA+TGG 0.554244 4.3:-18403259 MS.gene023200:CDS
GATTGCATTTCAATTGGACC+CGG 0.555852 4.3:-18403045 MS.gene023200:CDS
TACAACTCCTTAATAAGCAA+TGG 0.557489 4.3:-18403202 MS.gene023200:intron
TTGAAGACTTAGAAGGAACA+AGG 0.561772 4.3:+18404791 None:intergenic
CACCTGCATTCAAGAAAGCA+TGG 0.563512 4.3:-18404630 MS.gene023200:CDS
CTTAATTTGAGAAATGTGGA+TGG 0.570049 4.3:-18404853 MS.gene023200:CDS
TTGAAGGAAATGGAGATCAA+TGG 0.574015 4.3:-18404177 MS.gene023200:CDS
TTTACCCTGTGAGGTTTGCA+GGG 0.576342 4.3:+18404145 None:intergenic
TCATTTGGATAAGGGTCAGA+AGG 0.578630 4.3:+18404730 None:intergenic
ATGTACCTAATCCCGTGGCT+AGG 0.592436 4.3:+18402985 None:intergenic
CCAATACTATTGTTTGACAA+AGG 0.596001 4.3:-18402534 MS.gene023200:CDS
GGAAGGTGACCCTGAGACTG+TGG 0.598346 4.3:-18402267 MS.gene023200:CDS
TTAAATATCATGTGGAACTG+AGG 0.598983 4.3:+18403328 None:intergenic
TCTTCCCTGCAAACCTCACA+GGG 0.600082 4.3:-18404149 MS.gene023200:intron
AGGCAGATAGCAGAAATCAA+TGG 0.605028 4.3:-18404249 MS.gene023200:CDS
TGGACCCGGGACTTCAAATG+TGG 0.605054 4.3:-18403031 MS.gene023200:CDS
CTCACAGCTGGACTTCCACA+TGG 0.605942 4.3:+18403889 None:intergenic
TCTTATGACTGTGTCACGGA+CGG 0.610881 4.3:+18402670 None:intergenic
CTAAGAATAAAGACATGGCA+AGG 0.613229 4.3:-18402632 MS.gene023200:CDS
GGTATGCTAATGGCACCAGA+TGG 0.619104 4.3:-18404289 MS.gene023200:CDS
GGCAGCCATTAAATATCATG+TGG 0.622918 4.3:+18403320 None:intergenic
CATGGATTCCATCAGTGTTG+GGG 0.624775 4.3:+18403251 None:intergenic
TAAGGGTCAGAAGGAACACT+AGG 0.625203 4.3:+18404739 None:intergenic
ATTGCATTTCAATTGGACCC+GGG 0.630537 4.3:-18403044 MS.gene023200:CDS
TGAAGACTTAGAAGGAACAA+GGG 0.632788 4.3:+18404792 None:intergenic
ACACTAGGAGAAGAGTTGGA+AGG 0.640153 4.3:+18404754 None:intergenic
AAAGATCACCCACAGTCTCA+GGG 0.643345 4.3:+18402258 None:intergenic
ATGTAATGTACCTAATCCCG+TGG 0.645529 4.3:+18402980 None:intergenic
TGGACCTGATTCATGGCCAG+AGG 0.650317 4.3:-18404269 MS.gene023200:CDS
GAAATAGCTGGTGTAACTTG+TGG 0.654498 4.3:-18403009 MS.gene023200:CDS
GTCATAAGAGAATCAGACAA+TGG 0.654639 4.3:-18402656 MS.gene023200:CDS
ATTCTCTTATGACTGTGTCA+CGG 0.659505 4.3:+18402666 None:intergenic
AAAACTTTGTCAGGACCATG+TGG 0.660723 4.3:-18403904 MS.gene023200:CDS
AGGAAATGGAGATCAATGGT+GGG 0.664389 4.3:-18404173 MS.gene023200:CDS
TACAAACATAACACTCTCTG+AGG 0.682981 4.3:-18402393 MS.gene023200:CDS
AGAATAAAGACATGGCAAGG+TGG 0.690276 4.3:-18402629 MS.gene023200:CDS
CAGTCCGAAGACGAATCCCG+AGG 0.710358 4.3:+18404706 None:intergenic
ATGGACTAAGAATAAAGACA+TGG 0.715833 4.3:-18402637 MS.gene023200:CDS
GAAGGTGACCCTGAGACTGT+GGG 0.722175 4.3:-18402266 MS.gene023200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAAATAAATGATATATA+TGG + chr4.3:18402677-18402696 None:intergenic 10.0%
!! ATTCTATATGTAATTAATAT+AGG - chr4.3:18403300-18403319 MS.gene023200:CDS 10.0%
!! TCTATAAATAAATATTTACA+TGG + chr4.3:18403047-18403066 None:intergenic 10.0%
!! TTCTATATGTAATTAATATA+GGG - chr4.3:18403301-18403320 MS.gene023200:CDS 10.0%
!!! ATATCCTATTTTTATTTTTA+AGG - chr4.3:18403614-18403633 MS.gene023200:intron 10.0%
!!! ATATTTTGAATAGAAAAATT+AGG + chr4.3:18404263-18404282 None:intergenic 10.0%
!!! TAAAATTCGTTTTATTTTTA+GGG - chr4.3:18403645-18403664 MS.gene023200:intron 10.0%
!!! TTAAAATTCGTTTTATTTTT+AGG - chr4.3:18403644-18403663 MS.gene023200:intron 10.0%
!! AACAAAATTGAAATTTAATC+AGG - chr4.3:18402819-18402838 MS.gene023200:intron 15.0%
!! ATGTCAAAATTATATCAATT+AGG - chr4.3:18403555-18403574 MS.gene023200:intron 15.0%
!! TAGTCCTTAAAAATAAAAAT+AGG + chr4.3:18403621-18403640 None:intergenic 15.0%
!! TCAACTTAATAAAATATGAT+TGG - chr4.3:18402731-18402750 MS.gene023200:intron 15.0%
!! TGTCAAAATTATATCAATTA+GGG - chr4.3:18403556-18403575 MS.gene023200:intron 15.0%
!!! AAAATTCGTTTTATTTTTAG+GGG - chr4.3:18403646-18403665 MS.gene023200:intron 15.0%
!!! AAAATTTTCCATTTTGTTTT+TGG - chr4.3:18403409-18403428 MS.gene023200:intron 15.0%
!! AAAATTGAAATTTAATCAGG+TGG - chr4.3:18402822-18402841 MS.gene023200:intron 20.0%
!! AAAATTTGAGAACATACAAA+TGG - chr4.3:18404551-18404570 MS.gene023200:CDS 20.0%
!! AACATAGAAACAATAAAACT+GGG + chr4.3:18403995-18404014 None:intergenic 20.0%
!! ACATTAGCATAAACATAAAT+TGG + chr4.3:18403445-18403464 None:intergenic 20.0%
!! ATAAACATAAATTGGTCATT+AGG + chr4.3:18403437-18403456 None:intergenic 20.0%
!! GACATTTCTTTAATATAACA+TGG + chr4.3:18403529-18403548 None:intergenic 20.0%
!! TAAACATAAATTGGTCATTA+GGG + chr4.3:18403436-18403455 None:intergenic 20.0%
!! TAACATAGAAACAATAAAAC+TGG + chr4.3:18403996-18404015 None:intergenic 20.0%
!! TAATAAGCAATGGTAATTAA+TGG - chr4.3:18403944-18403963 MS.gene023200:intron 20.0%
!! TTAATTACCATTGCTTATTA+AGG + chr4.3:18403944-18403963 None:intergenic 20.0%
!! TTACTATTGACAAAATTTAG+AGG - chr4.3:18404282-18404301 MS.gene023200:CDS 20.0%
!! TTCATAGACTATTTATTCTT+TGG - chr4.3:18402245-18402264 MS.gene023200:CDS 20.0%
!! TTTCATATCTATAGATGATA+CGG - chr4.3:18403734-18403753 MS.gene023200:intron 20.0%
!!! AAAATGCCAAGTTTTATTTT+TGG + chr4.3:18403197-18403216 None:intergenic 20.0%
!!! AAATGAAACAAAAATTTTCG+AGG - chr4.3:18403584-18403603 MS.gene023200:intron 20.0%
!!! AATGAAACAAAAATTTTCGA+GGG - chr4.3:18403585-18403604 MS.gene023200:intron 20.0%
!!! ACAAAATGGAAAATTTTGAT+AGG + chr4.3:18403406-18403425 None:intergenic 20.0%
!!! ACATCTCGTTTTTATTTTTA+TGG + chr4.3:18403377-18403396 None:intergenic 20.0%
!!! ATAGTGTAATTTGTTTTACA+CGG - chr4.3:18403101-18403120 MS.gene023200:intron 20.0%
!!! ATCACTTCAATTATTTTTTC+AGG - chr4.3:18402589-18402608 MS.gene023200:CDS 20.0%
!!! ATCATAAAAGTGTAATTTTC+TGG + chr4.3:18402571-18402590 None:intergenic 20.0%
! ACCTCCAATTAATTGTTTAA+GGG - chr4.3:18404848-18404867 MS.gene023200:CDS 25.0%
! CACTAACCAAAAATAAAACT+TGG - chr4.3:18403188-18403207 MS.gene023200:intron 25.0%
! CTGGGTAAGTAAATTAATAT+TGG + chr4.3:18403977-18403996 None:intergenic 25.0%
! TACCTCCAATTAATTGTTTA+AGG - chr4.3:18404847-18404866 MS.gene023200:CDS 25.0%
! TGAATAGAAAAATTAGGATC+TGG + chr4.3:18404257-18404276 None:intergenic 25.0%
! TTAGAGGTGAAATATGAAAT+CGG - chr4.3:18404298-18404317 MS.gene023200:CDS 25.0%
! TTTAATATAACATGGACTCA+GGG + chr4.3:18403521-18403540 None:intergenic 25.0%
!! AAATAAAACTTGGCATTTTC+AGG - chr4.3:18403198-18403217 MS.gene023200:intron 25.0%
!! AATAAAACTTGGCATTTTCA+GGG - chr4.3:18403199-18403218 MS.gene023200:intron 25.0%
!! ACTACTTAATTTGAGAAATG+TGG - chr4.3:18402279-18402298 MS.gene023200:CDS 25.0%
!! ATTTTCGATGTTACATCATT+TGG - chr4.3:18402454-18402473 MS.gene023200:CDS 25.0%
!! TTGTTAATTTTGTACAGCAT+AGG - chr4.3:18404399-18404418 MS.gene023200:intron 25.0%
!!! AACAATAGTATTGGTCTATA+AGG + chr4.3:18404596-18404615 None:intergenic 25.0%
!!! AACATGATTTGATTTTGAAC+AGG - chr4.3:18404064-18404083 MS.gene023200:intron 25.0%
!!! CAAGTTTTATTTTTGGTTAG+TGG + chr4.3:18403190-18403209 None:intergenic 25.0%
AACATACAAATGGAGAATGT+AGG - chr4.3:18404561-18404580 MS.gene023200:CDS 30.0%
ACATATTGATCATTACTCCT+TGG + chr4.3:18403840-18403859 None:intergenic 30.0%
ACTAAACTTCGTGACAAAAT+TGG + chr4.3:18404327-18404346 None:intergenic 30.0%
ATTAGGGACCAAAAACAAAA+TGG + chr4.3:18403420-18403439 None:intergenic 30.0%
CAGTTCCACATGATATTTAA+TGG - chr4.3:18403811-18403830 MS.gene023200:intron 30.0%
CATAAGCATTCCAACAAAAA+GGG + chr4.3:18404806-18404825 None:intergenic 30.0%
CCAATACTATTGTTTGACAA+AGG - chr4.3:18404602-18404621 MS.gene023200:CDS 30.0%
CCAATTAATTGTTTAAGGGA+AGG - chr4.3:18404852-18404871 MS.gene023200:CDS 30.0%
CCTTCCCTTAAACAATTAAT+TGG + chr4.3:18404855-18404874 None:intergenic 30.0%
CCTTTGTCAAACAATAGTAT+TGG + chr4.3:18404605-18404624 None:intergenic 30.0%
CTGTACCATTAAAACTCATT+TGG + chr4.3:18402933-18402952 None:intergenic 30.0%
CTTACTTGAAATACTAATCC+TGG + chr4.3:18402625-18402644 None:intergenic 30.0%
CTTTAATATAACATGGACTC+AGG + chr4.3:18403522-18403541 None:intergenic 30.0%
GCTGCTTGCTTATATAAAAA+TGG + chr4.3:18404034-18404053 None:intergenic 30.0%
GTGTCCTACAAAAACATTAA+GGG - chr4.3:18404663-18404682 MS.gene023200:CDS 30.0%
TACAACTCCTTAATAAGCAA+TGG - chr4.3:18403934-18403953 MS.gene023200:intron 30.0%
TAGTCCATTCACATACAAAT+TGG + chr4.3:18403773-18403792 None:intergenic 30.0%
TATTTAATGACTGAGCTGAA+TGG + chr4.3:18403278-18403297 None:intergenic 30.0%
TCACTACAAGCAAAATGAAT+AGG + chr4.3:18404711-18404730 None:intergenic 30.0%
TCCCTTAAACAATTAATTGG+AGG + chr4.3:18404852-18404871 None:intergenic 30.0%
TGTGTCCTACAAAAACATTA+AGG - chr4.3:18404662-18404681 MS.gene023200:CDS 30.0%
TTAAATATCATGTGGAACTG+AGG + chr4.3:18403811-18403830 None:intergenic 30.0%
TTAATATAACATGGACTCAG+GGG + chr4.3:18403520-18403539 None:intergenic 30.0%
TTGAAAACTCACAAGATGTT+GGG - chr4.3:18403908-18403927 MS.gene023200:CDS 30.0%
TTGAAATTTAATCAGGTGGA+TGG - chr4.3:18402826-18402845 MS.gene023200:intron 30.0%
! ATGGACTAAGAATAAAGACA+TGG - chr4.3:18404499-18404518 MS.gene023200:intron 30.0%
! CTTAATTTGAGAAATGTGGA+TGG - chr4.3:18402283-18402302 MS.gene023200:CDS 30.0%
!! CAAATGAGTTTTAATGGTAC+AGG - chr4.3:18402931-18402950 MS.gene023200:intron 30.0%
!! CTTGACCAAATGAGTTTTAA+TGG - chr4.3:18402925-18402944 MS.gene023200:intron 30.0%
!! TGTACCCTTAATGTTTTTGT+AGG + chr4.3:18404670-18404689 None:intergenic 30.0%
ACTTCAAATGTGGAAATAGC+TGG - chr4.3:18404115-18404134 MS.gene023200:intron 35.0%
AGCTCCAATTTGTATGTGAA+TGG - chr4.3:18403766-18403785 MS.gene023200:intron 35.0%
ATGATATTTAATGGCTGCCA+AGG - chr4.3:18403820-18403839 MS.gene023200:intron 35.0%
ATTCTCTTATGACTGTGTCA+CGG + chr4.3:18404473-18404492 None:intergenic 35.0%
ATTGGACGTAAGTGTAAAAC+TGG - chr4.3:18402749-18402768 MS.gene023200:intron 35.0%
ATTTGAGAAATGTGGATGGA+AGG - chr4.3:18402287-18402306 MS.gene023200:CDS 35.0%
CCATAAGCATTCCAACAAAA+AGG + chr4.3:18404807-18404826 None:intergenic 35.0%
CTAAGAATAAAGACATGGCA+AGG - chr4.3:18404504-18404523 MS.gene023200:intron 35.0%
GGATCATTTGAAGACTTAGA+AGG + chr4.3:18402355-18402374 None:intergenic 35.0%
GGTGATGATTGCATTTCAAT+TGG - chr4.3:18404085-18404104 MS.gene023200:intron 35.0%
GTCATAAGAGAATCAGACAA+TGG - chr4.3:18404480-18404499 MS.gene023200:intron 35.0%
GTTGAAAACTCACAAGATGT+TGG - chr4.3:18403907-18403926 MS.gene023200:CDS 35.0%
TAATATAACATGGACTCAGG+GGG + chr4.3:18403519-18403538 None:intergenic 35.0%
TACAAACATAACACTCTCTG+AGG - chr4.3:18404743-18404762 MS.gene023200:CDS 35.0%
TCAAAAAAGTTTACCCTGTG+AGG + chr4.3:18403003-18403022 None:intergenic 35.0%
TCCAACAATTCACCTTCAAA+TGG + chr4.3:18404780-18404799 None:intergenic 35.0%
TGAAGACTTAGAAGGAACAA+GGG + chr4.3:18402347-18402366 None:intergenic 35.0%
TTGAAGACTTAGAAGGAACA+AGG + chr4.3:18402348-18402367 None:intergenic 35.0%
TTGAAGGAAATGGAGATCAA+TGG - chr4.3:18402959-18402978 MS.gene023200:intron 35.0%
TTGATCTCCATTTCCTTCAA+TGG + chr4.3:18402959-18402978 None:intergenic 35.0%
! AATACTAATCCTGGTTTGCA+TGG + chr4.3:18402616-18402635 None:intergenic 35.0%
! CATCTTGTGAGTTTTCAACA+TGG + chr4.3:18403906-18403925 None:intergenic 35.0%
! CCTTTTTGTTGGAATGCTTA+TGG - chr4.3:18404804-18404823 MS.gene023200:CDS 35.0%
! GGAAGATACTTTCTTTTGCT+TGG + chr4.3:18402326-18402345 None:intergenic 35.0%
! GTTACATCATTTGGAGCAAT+TGG - chr4.3:18402463-18402482 MS.gene023200:CDS 35.0%
! TAAGTTTGAGACACATGCTT+GGG + chr4.3:18404445-18404464 None:intergenic 35.0%
! TATTTCCACATTTGAAGTCC+CGG + chr4.3:18404113-18404132 None:intergenic 35.0%
! TTAAGTTTGAGACACATGCT+TGG + chr4.3:18404446-18404465 None:intergenic 35.0%
AAAACTTTGTCAGGACCATG+TGG - chr4.3:18403232-18403251 MS.gene023200:CDS 40.0%
AACATGGATTCCATCAGTGT+TGG + chr4.3:18403890-18403909 None:intergenic 40.0%
AAGGAAATGGAGATCAATGG+TGG - chr4.3:18402962-18402981 MS.gene023200:intron 40.0%
AATGGTACAGGAACCATTGA+AGG - chr4.3:18402943-18402962 MS.gene023200:intron 40.0%
ACAGGAACCATTGAAGGAAA+TGG - chr4.3:18402949-18402968 MS.gene023200:intron 40.0%
ACATGGATTCCATCAGTGTT+GGG + chr4.3:18403889-18403908 None:intergenic 40.0%
ACCCTTATCCAAATGATCCT+CGG - chr4.3:18402413-18402432 MS.gene023200:CDS 40.0%
AGAATAAAGACATGGCAAGG+TGG - chr4.3:18404507-18404526 MS.gene023200:intron 40.0%
AGGAAATGGAGATCAATGGT+GGG - chr4.3:18402963-18402982 MS.gene023200:intron 40.0%
AGGCAGATAGCAGAAATCAA+TGG - chr4.3:18402887-18402906 MS.gene023200:intron 40.0%
ATGTAATGTACCTAATCCCG+TGG + chr4.3:18404159-18404178 None:intergenic 40.0%
ATTGCATTTCAATTGGACCC+GGG - chr4.3:18404092-18404111 MS.gene023200:intron 40.0%
CACTACCAAGTGAAACAACA+TGG - chr4.3:18404219-18404238 MS.gene023200:CDS 40.0%
CCAGATGGAAAAACTTTGTC+AGG - chr4.3:18403223-18403242 MS.gene023200:CDS 40.0%
GAAATAGCTGGTGTAACTTG+TGG - chr4.3:18404127-18404146 MS.gene023200:intron 40.0%
GATTGCATTTCAATTGGACC+CGG - chr4.3:18404091-18404110 MS.gene023200:intron 40.0%
GCCATTTGAAGGTGAATTGT+TGG - chr4.3:18404776-18404795 MS.gene023200:CDS 40.0%
GGCAGCCATTAAATATCATG+TGG + chr4.3:18403819-18403838 None:intergenic 40.0%
TCATTTGGATAAGGGTCAGA+AGG + chr4.3:18402409-18402428 None:intergenic 40.0%
TTCCATGCTTTCTTGAATGC+AGG + chr4.3:18402511-18402530 None:intergenic 40.0%
TTGTACAGCATAGGAAGTCT+TGG - chr4.3:18404408-18404427 MS.gene023200:intron 40.0%
! ATTTCCACATTTGAAGTCCC+GGG + chr4.3:18404112-18404131 None:intergenic 40.0%
! CCTGACAAAGTTTTTCCATC+TGG + chr4.3:18403226-18403245 None:intergenic 40.0%
! GAAGAGTTGGAAGGATTTTC+TGG + chr4.3:18402376-18402395 None:intergenic 40.0%
! TCATTTGGAGCAATTGGAGA+TGG - chr4.3:18402469-18402488 MS.gene023200:CDS 40.0%
! TGTTGGATGACCCTTTTTGT+TGG - chr4.3:18404793-18404812 MS.gene023200:CDS 40.0%
!! GCAAACCATGTTGTTTCACT+TGG + chr4.3:18404227-18404246 None:intergenic 40.0%
!! GCTAAGTTTTGGTGCAGAAA+TGG + chr4.3:18403866-18403885 None:intergenic 40.0%
!! GTTGAGCTTTTGCCATTTGA+AGG - chr4.3:18404765-18404784 MS.gene023200:CDS 40.0%
!! TGAATGGTTTTACTCACAGC+TGG + chr4.3:18403262-18403281 None:intergenic 40.0%
!!! TCTTTTGCTTGGTGTGAAAG+TGG + chr4.3:18402315-18402334 None:intergenic 40.0%
AAACTTAGCCCCAACACTGA+TGG - chr4.3:18403877-18403896 MS.gene023200:intron 45.0%
AAAGACATGGCAAGGTGGAA+TGG - chr4.3:18404512-18404531 MS.gene023200:intron 45.0%
AAAGATCACCCACAGTCTCA+GGG + chr4.3:18404881-18404900 None:intergenic 45.0%
AAGACATGGCAAGGTGGAAT+GGG - chr4.3:18404513-18404532 MS.gene023200:intron 45.0%
ACACTAGGAGAAGAGTTGGA+AGG + chr4.3:18402385-18402404 None:intergenic 45.0%
AGGAACACTAGGAGAAGAGT+TGG + chr4.3:18402389-18402408 None:intergenic 45.0%
CACCTGCATTCAAGAAAGCA+TGG - chr4.3:18402506-18402525 MS.gene023200:CDS 45.0%
CATGGATTCCATCAGTGTTG+GGG + chr4.3:18403888-18403907 None:intergenic 45.0%
CCCGAGGATCATTTGGATAA+GGG + chr4.3:18402417-18402436 None:intergenic 45.0%
CCCTTATCCAAATGATCCTC+GGG - chr4.3:18402414-18402433 MS.gene023200:CDS 45.0%
TAAGGGTCAGAAGGAACACT+AGG + chr4.3:18402400-18402419 None:intergenic 45.0%
TCAGGTGGATGGTATGCTAA+TGG - chr4.3:18402837-18402856 MS.gene023200:intron 45.0%
TCCCGAGGATCATTTGGATA+AGG + chr4.3:18402418-18402437 None:intergenic 45.0%
TCTTATGACTGTGTCACGGA+CGG + chr4.3:18404469-18404488 None:intergenic 45.0%
TGATTTCTGCTATCTGCCTC+TGG + chr4.3:18402886-18402905 None:intergenic 45.0%
TGTAGAGTCAGGAGTTCTTC+TGG - chr4.3:18402543-18402562 MS.gene023200:CDS 45.0%
TTTACCCTGTGAGGTTTGCA+GGG + chr4.3:18402994-18403013 None:intergenic 45.0%
!! TCAGTGTTGGGGCTAAGTTT+TGG + chr4.3:18403877-18403896 None:intergenic 45.0%
!!! TTTTCAGGTCCATGCAAACC+AGG - chr4.3:18402604-18402623 MS.gene023200:CDS 45.0%
ATCTTCCCTGCAAACCTCAC+AGG - chr4.3:18402986-18403005 MS.gene023200:intron 50.0%
ATGTACCTAATCCCGTGGCT+AGG + chr4.3:18404154-18404173 None:intergenic 50.0%
CACCAGATGGACCTGATTCA+TGG - chr4.3:18402860-18402879 MS.gene023200:intron 50.0%
GAAAGATCACCCACAGTCTC+AGG + chr4.3:18404882-18404901 None:intergenic 50.0%
GACGAATCCCGAGGATCATT+TGG + chr4.3:18402424-18402443 None:intergenic 50.0%
GGTATGCTAATGGCACCAGA+TGG - chr4.3:18402847-18402866 MS.gene023200:intron 50.0%
GTTTACCCTGTGAGGTTTGC+AGG + chr4.3:18402995-18403014 None:intergenic 50.0%
TATAACATGGACTCAGGGGG+TGG + chr4.3:18403516-18403535 None:intergenic 50.0%
TCTTCCCTGCAAACCTCACA+GGG - chr4.3:18402987-18403006 MS.gene023200:intron 50.0%
TGATCCTCGGGATTCGTCTT+CGG - chr4.3:18402426-18402445 MS.gene023200:CDS 50.0%
! GTAACTTGTGGTCCTAGCCA+CGG - chr4.3:18404139-18404158 MS.gene023200:intron 50.0%
! TAACTTGTGGTCCTAGCCAC+GGG - chr4.3:18404140-18404159 MS.gene023200:intron 50.0%
!! TGGCATTTTCAGGGTCCAGA+TGG - chr4.3:18403208-18403227 MS.gene023200:CDS 50.0%
CTCACAGCTGGACTTCCACA+TGG + chr4.3:18403250-18403269 None:intergenic 55.0%
GAAGGTGACCCTGAGACTGT+GGG - chr4.3:18404870-18404889 MS.gene023200:CDS 55.0%
GCAGCTTGTGCTGTAGAGTC+AGG - chr4.3:18402532-18402551 MS.gene023200:CDS 55.0%
GGCCATGAATCAGGTCCATC+TGG + chr4.3:18402865-18402884 None:intergenic 55.0%
TCTGCCTCTGGCCATGAATC+AGG + chr4.3:18402874-18402893 None:intergenic 55.0%
TGGACCCGGGACTTCAAATG+TGG - chr4.3:18404105-18404124 MS.gene023200:intron 55.0%
TGGACCTGATTCATGGCCAG+AGG - chr4.3:18402867-18402886 MS.gene023200:intron 55.0%
CAGTCCGAAGACGAATCCCG+AGG + chr4.3:18402433-18402452 None:intergenic 60.0%
GGAAGGTGACCCTGAGACTG+TGG - chr4.3:18404869-18404888 MS.gene023200:CDS 60.0%
GTGGTCCTAGCCACGGGATT+AGG - chr4.3:18404146-18404165 MS.gene023200:intron 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 18402235 18404923 18402235 ID=MS.gene023200
chr4.3 mRNA 18402235 18404923 18402235 ID=MS.gene023200.t1;Parent=MS.gene023200
chr4.3 exon 18404519 18404923 18404519 ID=MS.gene023200.t1.exon1;Parent=MS.gene023200.t1
chr4.3 CDS 18404519 18404923 18404519 ID=cds.MS.gene023200.t1;Parent=MS.gene023200.t1
chr4.3 exon 18404150 18404317 18404150 ID=MS.gene023200.t1.exon2;Parent=MS.gene023200.t1
chr4.3 CDS 18404150 18404317 18404150 ID=cds.MS.gene023200.t1;Parent=MS.gene023200.t1
chr4.3 exon 18403894 18403938 18403894 ID=MS.gene023200.t1.exon3;Parent=MS.gene023200.t1
chr4.3 CDS 18403894 18403938 18403894 ID=cds.MS.gene023200.t1;Parent=MS.gene023200.t1
chr4.3 exon 18403203 18403410 18403203 ID=MS.gene023200.t1.exon4;Parent=MS.gene023200.t1
chr4.3 CDS 18403203 18403410 18403203 ID=cds.MS.gene023200.t1;Parent=MS.gene023200.t1
chr4.3 exon 18402991 18403072 18402991 ID=MS.gene023200.t1.exon5;Parent=MS.gene023200.t1
chr4.3 CDS 18402991 18403072 18402991 ID=cds.MS.gene023200.t1;Parent=MS.gene023200.t1
chr4.3 exon 18402235 18402742 18402235 ID=MS.gene023200.t1.exon6;Parent=MS.gene023200.t1
chr4.3 CDS 18402235 18402742 18402235 ID=cds.MS.gene023200.t1;Parent=MS.gene023200.t1
Gene Sequence

>MS.gene023200

ATGGATCTCATTCATAGACTATTTATTCTTTGGATTACATTTATACTACTTAATTTGAGAAATGTGGATGGAAGGAACCACTTTCACACCAAGCAAAAGAAAGTATCTTCCCTTGTTCCTTCTAAGTCTTCAAATGATCCAGAAAATCCTTCCAACTCTTCTCCTAGTGTTCCTTCTGACCCTTATCCAAATGATCCTCGGGATTCGTCTTCGGACTGTATTTTCGATGTTACATCATTTGGAGCAATTGGAGATGGTTCAGCTGATGACACACCTGCATTCAAGAAAGCATGGAAAGCAGCTTGTGCTGTAGAGTCAGGAGTTCTTCTGGTTCCAGAAAATTACACTTTTATGATCACTTCAATTATTTTTTCAGGTCCATGCAAACCAGGATTAGTATTTCAAGTAAGTAAGATAACTTTTTCAATTTCTAATTTCACCATATATATCATTTATTTTGTTACAAACAAATTATTATTTCTTAAATGCATAAATATCAACTTAATAAAATATGATTGGACGTAAGTGTAAAACTGGTTTAACGTTAGTGCGAATTCTTACTATTATAATAATATGAAATTATTAACAAAATTGAAATTTAATCAGGTGGATGGTATGCTAATGGCACCAGATGGACCTGATTCATGGCCAGAGGCAGATAGCAGAAATCAATGGCTTGTGTTCTATAAACTTGACCAAATGAGTTTTAATGGTACAGGAACCATTGAAGGAAATGGAGATCAATGGTGGGATCTTCCCTGCAAACCTCACAGGGTAAACTTTTTTGAACATTATTCAAGCATATCTCACCATGTAAATATTTATTTATAGAGATATTTTAAAAACTAATTTAAGAAAGTGACATTATAGTGTAATTTGTTTTACACGGTGAATGTGTACAAATTAATTTCTATTACTATTGTTGTATGCATCTAATGTTGATTGATACACACCACTAACCAAAAATAAAACTTGGCATTTTCAGGGTCCAGATGGAAAAACTTTGTCAGGACCATGTGGAAGTCCAGCTGTGAGTAAAACCATTCAGCTCAGTCATTAAATAACATTCTATATGTAATTAATATAGGGTGAAATAAATTTTTTTATCGTAAAAATATAGAAATTTTTGATTATATAGTCCATAAAAATAAAAACGAGATGTTTTCTTCCTATCAAAATTTTCCATTTTGTTTTTGGTCCCTAATGACCAATTTATGTTTATGCTAATGTCAACATAATTATGATCATTTTTATGTTTCAGATGTATTCAATGTATCTCCACCCCCTGAGTCCATGTTATATTAAAGAAATGTCAAAAACATGTCAAAATTATATCAATTAGGGACAAAAAATGAAACAAAAATTTTCGAGGGACAAAAATATCCTATTTTTATTTTTAAGGACTAAAATTAAAATTCGTTTTATTTTTAGGGGAAAAAAAACTTATTTAATTCAATAATATATATATTACTAGCTAGTGTCTTAAAGTTGTTTTTAATTTTCATATCTATAGATGATACGGTTCTTCATGAGCTCCAATTTGTATGTGAATGGACTAAAAATTCAAAACAGTCCTCAGTTCCACATGATATTTAATGGCTGCCAAGGAGTAATGATCAATATGTTATCCATTTCTGCACCAAAACTTAGCCCCAACACTGATGGAATCCATGTTGAAAACTCACAAGATGTTGGGATATACAACTCCTTAATAAGCAATGGTAATTAATGGCTATAATCCAATATTAATTTACTTACCCAGTTTTATTGTTTCTATGTTAATTTTATTAATTTTACCATTTTTATATAAGCAAGCAGCTTAATATAATAACATGATTTGATTTTGAACAGGTGATGATTGCATTTCAATTGGACCCGGGACTTCAAATGTGGAAATAGCTGGTGTAACTTGTGGTCCTAGCCACGGGATTAGGTACATTACATAATCTATTCTTGTTTTGTTGATTAATAAAAAAATTAGTGTCACTACCAAGTGAAACAACATGGTTTGCAACAATACCAGATCCTAATTTTTCTATTCAAAATATTACTATTGACAAAATTTAGAGGTGAAATATGAAATCGGTCTCCAATTTTGTCACGAAGTTTAGTTTAGTTTAATTTTCTGTAGTACATATCAGCATGAGTCACTAAACTTAATGTTTTGTTAATTTTGTACAGCATAGGAAGTCTTGGTGTGCACAATTCCCAAGCATGTGTCTCAAACTTAACCGTCCGTGACACAGTCATAAGAGAATCAGACAATGGACTAAGAATAAAGACATGGCAAGGTGGAATGGGATCTGTGTCAAATTTAAAATTTGAGAACATACAAATGGAGAATGTAGGAAACTGCATCCTTATAGACCAATACTATTGTTTGACAAAGGAATGTCTAAATCAAACATCAGCAGTACATGTTAATGATGTGTCCTACAAAAACATTAAGGGTACATATGATGTTAGAACTGCTCCTATTCATTTTGCTTGTAGTGACACTGTTGCTTGTACAAACATAACACTCTCTGAGGTTGAGCTTTTGCCATTTGAAGGTGAATTGTTGGATGACCCTTTTTGTTGGAATGCTTATGGAGATCAAGAGACTTTGACTATACCTCCAATTAATTGTTTAAGGGAAGGTGACCCTGAGACTGTGGGTGATCTTTCTACATATCAATGTAGTAATTAG
Protein sequence

>MS.gene023200.t1

MDLIHRLFILWITFILLNLRNVDGRNHFHTKQKKVSSLVPSKSSNDPENPSNSSPSVPSDPYPNDPRDSSSDCIFDVTSFGAIGDGSADDTPAFKKAWKAACAVESGVLLVPENYTFMITSIIFSGPCKPGLVFQVDGMLMAPDGPDSWPEADSRNQWLVFYKLDQMSFNGTGTIEGNGDQWWDLPCKPHRGPDGKTLSGPCGSPAMIRFFMSSNLYVNGLKIQNSPQFHMIFNGCQGVMINMLSISAPKLSPNTDGIHVENSQDVGIYNSLISNGDDCISIGPGTSNVEIAGVTCGPSHGISIGSLGVHNSQACVSNLTVRDTVIRESDNGLRIKTWQGGMGSVSNLKFENIQMENVGNCILIDQYYCLTKECLNQTSAVHVNDVSYKNIKGTYDVRTAPIHFACSDTVACTNITLSEVELLPFEGELLDDPFCWNAYGDQETLTIPPINCLREGDPETVGDLSTYQCSN