AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene025314

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025314.t1 MTR_2g043530 98.319 476 8 0 7 482 13 488 0.0 969
MS.gene025314.t1 MTR_2g043530 98.174 438 8 0 7 444 13 450 0.0 893
MS.gene025314.t1 MTR_4g124880 82.255 479 79 2 10 482 13 491 0.0 803
MS.gene025314.t1 MTR_4g124880 88.986 345 38 0 138 482 1 345 0.0 654
MS.gene025314.t1 MTR_1g103020 55.000 460 192 4 27 482 23 471 0.0 522
MS.gene025314.t1 MTR_4g096690 57.111 443 181 4 44 482 32 469 1.18e-180 514
MS.gene025314.t1 MTR_8g099695 52.036 442 210 2 41 481 40 480 6.07e-169 485
MS.gene025314.t1 MTR_8g099715 44.444 441 187 6 42 481 9 392 1.59e-128 379
MS.gene025314.t1 MTR_1g069385 40.774 439 233 7 66 479 32 468 1.85e-99 307
MS.gene025314.t1 MTR_1g069385 43.804 347 188 4 138 479 1 345 4.84e-88 273
MS.gene025314.t1 MTR_8g099700 45.817 251 109 2 60 309 65 289 5.39e-73 233
MS.gene025314.t1 MTR_8g099705 56.303 119 52 0 339 457 1 119 8.20e-38 135
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025314.t1 AT1G74960 84.842 442 66 1 41 482 101 541 0.0 773
MS.gene025314.t1 AT1G74960 84.842 442 66 1 41 482 101 541 0.0 773
MS.gene025314.t1 AT1G74960 84.842 442 66 1 41 482 101 541 0.0 773
MS.gene025314.t1 AT5G46290 54.626 454 202 3 30 482 22 472 0.0 518
MS.gene025314.t1 AT5G46290 52.979 470 201 4 30 482 22 488 3.35e-179 511
MS.gene025314.t1 AT5G46290 42.826 453 202 7 30 482 22 417 2.48e-105 320
MS.gene025314.t1 AT2G04540 38.621 435 237 6 70 479 30 459 8.19e-90 282

Find 124 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAGTTTACCTCAGGATTT+TGG 0.178284 2.2:+43822715 None:intergenic
ACCATTTGCAACAACAAATA+TGG 0.187806 2.2:-43827204 MS.gene025314:CDS
CAGCTTCACCTTTAATTATA+TGG 0.206063 2.2:+43826963 None:intergenic
CTCTGTTTCCAGAGGATTGC+TGG 0.218073 2.2:-43828912 MS.gene025314:intron
GTTGCAAATGGTACACAAAA+AGG 0.243023 2.2:+43827215 None:intergenic
GCTGCAAACCATATAATTAA+AGG 0.245748 2.2:-43826971 MS.gene025314:CDS
GAAAAGGCATTAGCTAATTC+TGG 0.245771 2.2:-43822834 MS.gene025314:CDS
GATTGGGAGAAGAGAGATTA+AGG 0.255347 2.2:+43830947 None:intergenic
TTGTCTAATTCATTTGGTTT+TGG 0.284201 2.2:-43821476 MS.gene025314:CDS
ACCGAGGAACTCTGCATATA+TGG 0.296883 2.2:+43825799 None:intergenic
GAAAGAGAGACTGGACATTA+AGG 0.307184 2.2:-43821504 MS.gene025314:CDS
TAAAGTAATGGCTGTGGCTT+TGG 0.323323 2.2:-43829185 MS.gene025314:CDS
GCTGTGGATATTGAATAGTT+TGG 0.338257 2.2:+43827025 None:intergenic
AAAATCTGTTTCACTTAAAA+GGG 0.341894 2.2:+43821426 None:intergenic
CCCATATTTGTTGTTGCAAA+TGG 0.365943 2.2:+43827203 None:intergenic
TACCTGCATTCCACCCATTC+CGG 0.366985 2.2:+43828716 None:intergenic
CAGGCTTTGATTCATTGCTT+TGG 0.368321 2.2:-43822738 MS.gene025314:CDS
AGTGGGATAAGCGAGATTAA+TGG 0.369472 2.2:-43829028 MS.gene025314:CDS
GCAGGCTTGTCTAATTCATT+TGG 0.370179 2.2:-43821482 MS.gene025314:CDS
TGGGTGGCTTTGTAGCATGC+AGG 0.380536 2.2:-43826674 MS.gene025314:CDS
CTGTGGATATTGAATAGTTT+GGG 0.381332 2.2:+43827026 None:intergenic
TTTGTAATGGGTGAAGGGGC+TGG 0.383948 2.2:-43826162 MS.gene025314:CDS
ACTACTCGTCTTTGCTCTAT+AGG 0.387655 2.2:+43829127 None:intergenic
CACACCTGCTGGAGATCTTA+AGG 0.393542 2.2:-43822766 MS.gene025314:CDS
TGCCATGCTTGCAATGGATC+TGG 0.395136 2.2:-43827177 MS.gene025314:intron
TCCATCCTCTTTGAAAACTT+TGG 0.397379 2.2:+43828852 None:intergenic
TACTAAAGCTTCACGCCCTT+GGG 0.399669 2.2:-43826625 MS.gene025314:CDS
TTGAAAATTGTAGGGGTGGA+TGG 0.404082 2.2:-43827050 MS.gene025314:intron
CCCAATCGAATGTCCCGTTC+TGG 0.406670 2.2:-43830931 MS.gene025314:intron
CCATTTGCAACAACAAATAT+GGG 0.422822 2.2:-43827203 MS.gene025314:CDS
TTCTACAATAATCTACTTGA+TGG 0.427157 2.2:-43829055 MS.gene025314:CDS
TATCATACCACTGCTTCATC+CGG 0.430956 2.2:+43822373 None:intergenic
TCTGTTTCCAGAGGATTGCT+GGG 0.438070 2.2:-43828911 MS.gene025314:intron
AAGCTTTACGAATCTCATAT+AGG 0.438138 2.2:-43827247 MS.gene025314:CDS
ATCGTTCTCGTCGTCGCAGC+AGG 0.440595 2.2:-43830978 MS.gene025314:CDS
AGATTATTGTAGAAGACATC+TGG 0.441095 2.2:+43829064 None:intergenic
TGTTATTCTTTGCATTGAAA+AGG 0.442258 2.2:-43822850 MS.gene025314:CDS
AAGTGTGAAATTACCAGAAC+GGG 0.446164 2.2:+43830918 None:intergenic
CATGCTCGTGGCGGACAGAT+TGG 0.447314 2.2:-43833750 MS.gene025314:CDS
CGAGTAGTTGTGACGGGTAT+GGG 0.449622 2.2:-43829112 MS.gene025314:CDS
AATCTTGGTGTAGGCTTGGG+TGG 0.449843 2.2:-43826690 MS.gene025314:intron
ACGAGTAGTTGTGACGGGTA+TGG 0.463031 2.2:-43829113 MS.gene025314:CDS
AATCTACTTGATGGTGTTAG+TGG 0.463552 2.2:-43829046 MS.gene025314:CDS
CTACTAAAGCTTCACGCCCT+TGG 0.463863 2.2:-43826626 MS.gene025314:CDS
ATCATTATTTCTTTGTGAGA+GGG 0.464240 2.2:+43826650 None:intergenic
TGAAAATTGTAGGGGTGGAT+GGG 0.466104 2.2:-43827049 MS.gene025314:intron
TTGCAGGACGTGATGCTTTG+TGG 0.471500 2.2:-43826813 MS.gene025314:intron
GGGTGGCTTTGTAGCATGCA+GGG 0.471985 2.2:-43826673 MS.gene025314:CDS
GGAAGCCGTTGCAACTATAC+AGG 0.479199 2.2:-43822542 MS.gene025314:intron
ACCAGAACGGGACATTCGAT+TGG 0.480965 2.2:+43830930 None:intergenic
ATGTTGTATCTGCTGACAGC+TGG 0.481211 2.2:-43828822 MS.gene025314:CDS
ATCAAATCTTTCTCAACTGA+TGG 0.487001 2.2:-43828885 MS.gene025314:CDS
CTCCGGAATGGGTGGAATGC+AGG 0.488440 2.2:-43828718 MS.gene025314:intron
CAAGGGCGTGAAGCTTTAGT+AGG 0.491204 2.2:+43826627 None:intergenic
CATGCCACATCCACACCTGC+TGG 0.494143 2.2:-43822777 MS.gene025314:CDS
GATGCTGCTATAATACCAAT+TGG 0.497951 2.2:-43826783 MS.gene025314:intron
AACCGTGATGGATTTGTAAT+GGG 0.498732 2.2:-43826174 MS.gene025314:CDS
TGAGATTCGTAAAGCTTCAA+TGG 0.501049 2.2:+43827253 None:intergenic
AGCTAATTCTGGAATTTCAA+AGG 0.506190 2.2:-43822823 MS.gene025314:CDS
CAGGACACCAATGTGCTTGT+GGG 0.506351 2.2:-43821533 MS.gene025314:CDS
GACCAGATCCATTGCAAGCA+TGG 0.507024 2.2:+43827175 None:intergenic
TTCATGTAGGCAATAAAGAC+AGG 0.508573 2.2:-43822427 MS.gene025314:intron
ATCTCCAGCAGGTGTGGATG+TGG 0.511310 2.2:+43822773 None:intergenic
TCTATGATTGGTCATCTACT+AGG 0.512790 2.2:-43822580 MS.gene025314:CDS
CCAGAACGGGACATTCGATT+GGG 0.522885 2.2:+43830931 None:intergenic
ATGGGTGTGGTCACACCACT+TGG 0.525435 2.2:-43829094 MS.gene025314:CDS
CTTTGATCCCACAAGCACAT+TGG 0.525820 2.2:+43821526 None:intergenic
CCAGGACACCAATGTGCTTG+TGG 0.525865 2.2:-43821534 MS.gene025314:intron
GATCATTATTTCTTTGTGAG+AGG 0.527650 2.2:+43826649 None:intergenic
AGAAGAGTTAGAGCATGCTA+AGG 0.527971 2.2:-43826127 MS.gene025314:intron
ATCATACCACTGCTTCATCC+GGG 0.529315 2.2:+43822374 None:intergenic
AATTCGACGAAGTCTATGAT+TGG 0.531883 2.2:-43822592 MS.gene025314:CDS
GGGATCAAAGAAAGAGAGAC+TGG 0.532804 2.2:-43821513 MS.gene025314:CDS
GGTCATCTACTAGGAGCAGC+TGG 0.536898 2.2:-43822571 MS.gene025314:CDS
GAACCGTGATGGATTTGTAA+TGG 0.538884 2.2:-43826175 MS.gene025314:CDS
AAAATTTCAAGGTAAAGTAA+TGG 0.539854 2.2:-43829197 MS.gene025314:intron
TGGAGATCTTAAGGAGTACC+AGG 0.543599 2.2:-43822757 MS.gene025314:CDS
GCTCGTGGCGGACAGATTGG+TGG 0.545345 2.2:-43833747 MS.gene025314:CDS
CTTAAGATCTCCAGCAGGTG+TGG 0.546013 2.2:+43822767 None:intergenic
GATGGATTTGTAATGGGTGA+AGG 0.551362 2.2:-43826168 MS.gene025314:CDS
ACTCGTCTTTGCTCTATAGG+AGG 0.556147 2.2:+43829130 None:intergenic
ACCATATATGCAGAGTTCCT+CGG 0.556868 2.2:-43825800 MS.gene025314:CDS
ATGGATTTGTAATGGGTGAA+GGG 0.560592 2.2:-43826167 MS.gene025314:CDS
CATAATATTACAATATCCCA+AGG 0.565400 2.2:+43826609 None:intergenic
AGTTGTGACGGGTATGGGTG+TGG 0.566633 2.2:-43829107 MS.gene025314:CDS
TTGGTGGATCAACCAAGTGC+AGG 0.568673 2.2:-43833731 MS.gene025314:intron
TCAAGGTAAAGTAATGGCTG+TGG 0.568925 2.2:-43829191 MS.gene025314:intron
TGGAATTACTGAAGAAGTAA+TGG 0.572336 2.2:-43828778 MS.gene025314:CDS
CTGTTTCCAGAGGATTGCTG+GGG 0.573853 2.2:-43828910 MS.gene025314:intron
CAAAGCAATGAATCAAAGCC+TGG 0.573865 2.2:+43822739 None:intergenic
ATCTACTTGATGGTGTTAGT+GGG 0.577119 2.2:-43829045 MS.gene025314:CDS
TGGATTTGTAATGGGTGAAG+GGG 0.577451 2.2:-43826166 MS.gene025314:CDS
GAGAGACTGGACATTAAGGC+AGG 0.582501 2.2:-43821500 MS.gene025314:CDS
CAAAGACGAGTAGTTGTGAC+GGG 0.584893 2.2:-43829118 MS.gene025314:CDS
CAATCTGTCCGCCACGAGCA+TGG 0.586128 2.2:+43833751 None:intergenic
GGGTTCTGCCATGCTTGCAA+TGG 0.587872 2.2:-43827183 MS.gene025314:CDS
TAATATCAATCTAGAAAACC+CGG 0.589028 2.2:-43822392 MS.gene025314:CDS
GAGTTAGATAAAACAAAATG+TGG 0.592751 2.2:-43828753 MS.gene025314:CDS
AAATCTGTTTCACTTAAAAG+GGG 0.593441 2.2:+43821427 None:intergenic
ACTAGGAGCAGCTGGTGGTG+TGG 0.593602 2.2:-43822563 MS.gene025314:CDS
TACTCCTTAAGATCTCCAGC+AGG 0.608811 2.2:+43822762 None:intergenic
TGGGAGAAGAGAGATTAAGG+TGG 0.610292 2.2:+43830950 None:intergenic
TGTCACCTGTATAGTTGCAA+CGG 0.612124 2.2:+43822537 None:intergenic
CAGGACGTGATGCTTTGTGG+TGG 0.615515 2.2:-43826810 MS.gene025314:CDS
GAAGTGTGAAATTACCAGAA+CGG 0.615575 2.2:+43830917 None:intergenic
TGATTGTGCACAATATCCAA+CGG 0.615962 2.2:-43829002 MS.gene025314:intron
TTGATTGGCTCCGGAATGGG+TGG 0.619435 2.2:-43828726 MS.gene025314:CDS
TTGCTTTGGCCAAAATCCTG+AGG 0.628055 2.2:-43822724 MS.gene025314:intron
GGGGAATGTATACCTGCACT+TGG 0.629702 2.2:+43833719 None:intergenic
AGAAAACCCGGATGAAGCAG+TGG 0.634880 2.2:-43822380 MS.gene025314:intron
ATTACTTGTAGCACACGCTG+TGG 0.636535 2.2:+43827009 None:intergenic
AGTAATTCCAGCATTGACCA+AGG 0.637726 2.2:+43828791 None:intergenic
GCAAAGACGAGTAGTTGTGA+CGG 0.640661 2.2:-43829119 MS.gene025314:CDS
GTGACTGAACCACGTCCTGA+TGG 0.644994 2.2:-43825749 MS.gene025314:intron
CATCTACTAGGAGCAGCTGG+TGG 0.649400 2.2:-43822568 MS.gene025314:CDS
CACCCATTACAAATCCATCA+CGG 0.660498 2.2:+43826172 None:intergenic
GTAGGCAATAAAGACAGGGT+GGG 0.660808 2.2:-43822422 MS.gene025314:intron
TGTAGGCAATAAAGACAGGG+TGG 0.666418 2.2:-43822423 MS.gene025314:intron
TAATTCTGGAATTTCAAAGG+AGG 0.680514 2.2:-43822820 MS.gene025314:CDS
ACATCTGGATCATGACCAAG+TGG 0.691170 2.2:+43829079 None:intergenic
ATAATATTACAATATCCCAA+GGG 0.702702 2.2:+43826610 None:intergenic
TCATGTAGGCAATAAAGACA+GGG 0.721427 2.2:-43822426 MS.gene025314:intron
ATATATGCAGAGTTCCTCGG+TGG 0.726227 2.2:-43825797 MS.gene025314:CDS
CACATGTGAAGCTTCCACCG+AGG 0.767036 2.2:+43825783 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr2.2 gene 43821436 43833771 43821436 ID=MS.gene025314
chr2.2 mRNA 43821436 43833771 43821436 ID=MS.gene025314.t1;Parent=MS.gene025314
chr2.2 exon 43833732 43833771 43833732 ID=MS.gene025314.t1.exon1;Parent=MS.gene025314.t1
chr2.2 CDS 43833732 43833771 43833732 ID=cds.MS.gene025314.t1;Parent=MS.gene025314.t1
chr2.2 exon 43830932 43831030 43830932 ID=MS.gene025314.t1.exon2;Parent=MS.gene025314.t1
chr2.2 CDS 43830932 43831030 43830932 ID=cds.MS.gene025314.t1;Parent=MS.gene025314.t1
chr2.2 exon 43829003 43829208 43829003 ID=MS.gene025314.t1.exon3;Parent=MS.gene025314.t1
chr2.2 CDS 43829003 43829208 43829003 ID=cds.MS.gene025314.t1;Parent=MS.gene025314.t1
chr2.2 exon 43828719 43828922 43828719 ID=MS.gene025314.t1.exon4;Parent=MS.gene025314.t1
chr2.2 CDS 43828719 43828922 43828719 ID=cds.MS.gene025314.t1;Parent=MS.gene025314.t1
chr2.2 exon 43827178 43827288 43827178 ID=MS.gene025314.t1.exon5;Parent=MS.gene025314.t1
chr2.2 CDS 43827178 43827288 43827178 ID=cds.MS.gene025314.t1;Parent=MS.gene025314.t1
chr2.2 exon 43826964 43827059 43826964 ID=MS.gene025314.t1.exon6;Parent=MS.gene025314.t1
chr2.2 CDS 43826964 43827059 43826964 ID=cds.MS.gene025314.t1;Parent=MS.gene025314.t1
chr2.2 exon 43826784 43826829 43826784 ID=MS.gene025314.t1.exon7;Parent=MS.gene025314.t1
chr2.2 CDS 43826784 43826829 43826784 ID=cds.MS.gene025314.t1;Parent=MS.gene025314.t1
chr2.2 exon 43826620 43826699 43826620 ID=MS.gene025314.t1.exon8;Parent=MS.gene025314.t1
chr2.2 CDS 43826620 43826699 43826620 ID=cds.MS.gene025314.t1;Parent=MS.gene025314.t1
chr2.2 exon 43826128 43826196 43826128 ID=MS.gene025314.t1.exon9;Parent=MS.gene025314.t1
chr2.2 CDS 43826128 43826196 43826128 ID=cds.MS.gene025314.t1;Parent=MS.gene025314.t1
chr2.2 exon 43825750 43825834 43825750 ID=MS.gene025314.t1.exon10;Parent=MS.gene025314.t1
chr2.2 CDS 43825750 43825834 43825750 ID=cds.MS.gene025314.t1;Parent=MS.gene025314.t1
chr2.2 exon 43822725 43822879 43822725 ID=MS.gene025314.t1.exon11;Parent=MS.gene025314.t1
chr2.2 CDS 43822725 43822879 43822725 ID=cds.MS.gene025314.t1;Parent=MS.gene025314.t1
chr2.2 exon 43822543 43822623 43822543 ID=MS.gene025314.t1.exon12;Parent=MS.gene025314.t1
chr2.2 CDS 43822543 43822623 43822543 ID=cds.MS.gene025314.t1;Parent=MS.gene025314.t1
chr2.2 exon 43822381 43822440 43822381 ID=MS.gene025314.t1.exon13;Parent=MS.gene025314.t1
chr2.2 CDS 43822381 43822440 43822381 ID=cds.MS.gene025314.t1;Parent=MS.gene025314.t1
chr2.2 exon 43821436 43821552 43821436 ID=MS.gene025314.t1.exon14;Parent=MS.gene025314.t1
chr2.2 CDS 43821436 43821552 43821436 ID=cds.MS.gene025314.t1;Parent=MS.gene025314.t1
Gene Sequence

>MS.gene025314

ATGCTCGTGGCGGACAGATTGGTGGATCAACCAAGTGCAGGTATACATTCCCCTTTGTCTCATCGGGAGCTGATGATTAACAAACCTCACAAGAATCATCACTTCATTGCAGTTCAACAGAACCATGCTCATGGCGGACAAATTGGTGGATCAACCAATGAAAACAGTAGATCCAAGTTGCTCAAACCATTCAAACTTGTTGTTCTTTCTTGCAATCTAAGCACCATAATCCTTGTTTCCTCATCCATCCTTTAGTTCCTATCTCCACTCAAAGCTTCCACCACCCAAAACCCTAACTTCTTGATTATTTCCTTGTGATGAGAAAGTTGGAAAACAAGCCCAAATGAAGCTTCTACTTGTTTCTAAATCTTGGAAGTACCATAACATAAGCTTATTCCTTGAGGGCAAGAAGTTTGCAAGCTTGATGAAGAGAGGGAGAGAGAGGGATGGCGTGAGAAGTAGAGAGAAGGAGGAAGAAATTCTTTGACAAAGCTTAGAGAGAGAAAGGATAAGGGAAAAATAAGATATTTTGGGCCAACACCAAATAAATATCTTTTCTCGTGCAATTACCAATTTGCCCTTGCCTTAGATGGGTTTATTACGACACTGATACTTTACCCGTATTAAGCTGACATCTTATAATAAATATTTCCGTCAAACTTTCTTCCCGAAGTATTAATCCTCTGGCATGATCGACTGGTAATATTCCGATACTGACAGGCGGGATATTACAGAGAGTTTGTGGTACAAAGTGTTGGCTGTATGTTATGACGAGGAAGGTATGAGGTTGTGTTTTGGTGGGAGAAGAGGGTTGGTGTGGTGGGAGAATTTGAAAAAAATTAGGACGGAGGCTGGTTTGGTGAATCAGGTGTGGTTAATTGACAATATTTCTCGGTAGGTTGGGAATGAGAGTGATACTTTGTTTTGGAGGGACTTGTGGCATGATGGGGTTTTATTAGAGGTTAGTTTTCGTAGACTTTTTGAGTTGGTGGAAAATAAATTAGTTATTGTTGCGGATGTGAAGGTGATGGGTCGGGATGAATCCGGGGAGGCTTAAAGGTGACATAAGAGGCTGTTTGCGTGGGAGGAAGAGTTGTTGAGGGAGTGTGTGGATAGGTTGTCTAATGTTATTTTGCAAGCGAAAATGAAGGACCGGTGGGCGTGGAATCTTCATTCGTCCAAACGGTACACCGTTAAAAGTGCTTACGAGAACTTGGCTTATGTAGATGTTGGTTATAAACACGTGTTCCGCTTAAATTCAATATTTTTGTTTGGAGATTGATGCTGAACAGTTTACCTCTTAAGGATCTCTTGGTTAGGCGTCATATTTTAGCATTATATGATAATATTTGCTATGATAATATTTGCTATGCTGATTGTGATTATGATGAAGATAAAGACCATCTCTTTGTAAATTGCAAGGTTTTTTTGTTCTCTTTGGTTAGTGATCTCAGGTTGGCTAGATATTTCAACAACTTTACATGGAAACATTCAGGATCATCTTTTATTGTTTGGCGATTTAGGGGGATTTTCTAAAAATTCTTGCATCGCTTTCAATACTATTTAGATTGTAGTTCTTTGGGTCATTTAGAAGGAAAGAAGCGAAAGAATTTTTCAACAAAAGAGTAATGTTATTCAGACTTTGAGTGAAAAGGTTAAACTTCATATTTATTAGTGGTTAAAATCGTATTATGTATCATTCCATTTTGTTTGATTATCTGTTTTAAAGGCAGAATCTGTTGTTTTTCTTAAGAGCTCTGTTGTAATTTTCATTTCGTCACTATGACTTCGGTTTCTTGTAATGTAACTTGTATGCATTGTTAAGCTCTTATAACACACCTTGTGCTAGATGTGCTTTTTGTGTGTTTTAATATAATTTCTTTAGCTTTTTCAAAAATGGGAGGGAGTATGGTGTTTATGGTGACATTTGAATCAATTTTAAGACAAATAATCATTCGATCAAAAATAAAAATCACATACGATTCCGATGACTATTATTTCTAATCATCATGATTTTTTTAAGCTGTTATGATTTTTTTTAAGCTGTTACGACATAACATTAAAGAATCATTCGATTATAAACAAAAATCACATTTGATTGTTAATCTGTTATGACACACCTTGTGAATTTTTTTTAATAGTCATCATGATTTTTTTTTTTTTTACCAAGGTTCTGAGTTCGAATATAACCTTATACATGCAACAGCGTTAAAACTCTTAGGAGAGTTTGCCGTCTATTTATGTCCCACAATGCTCGAAGGATTAATATTCGCAGTTGCACTCGTTAATACCCAGTTTACACCCAAAAATTTAATTTTTTTAAACTGAATAAATGAGATATTCACGATTTGAACCCGACATCTCCATAAACAAACGGGGATTGTGATTTTATTTTGGATTGATTTGGATCTGAATATAAAGTATATTAATTGATTATGATTATTATAATTAAAAAAATGTTATTAAAATGAGAGATTGACTATTTGTTAAGCAGTTAGTGATTTACTACTACTGTTGTACAGAACTCTGCTTCTCATCGCACCCAGTTCTCTTCTCTCACTCAAACACCAACCACAAAAATCAAAATCAAAATCAAAACAAAATTAACTAAAAAAACCTCTTAACCTTTCTTCCTTCATTCTTTCTTTCATCTCTCTCACACCATTCTCATTCATTTCTCTTCATGGCTTCTTCACTTACCTCTCCTCTCTGCACGTGGCTTGTTGCTGCTTGCATGTCAGTCACGTGTGACTCTCACTCTTCTCAATCTCATCGTTCTCGTCGTCGCAGCAGGAACAACCACCTTAATCTCTCTTCTCCCAATCGAATGTCCCGTTCTGGTAATTTCACACTTCTTTTTTCCTATTAATTACTCAATTTTCATTTTTTTTATTCGTTAATTCTGATGTTTATTTTTATTTTTCTTATTTATAGTTTATAATTAATTTTATATCATGATGAATATTCTCATAGTTGGTTTGAAACAATAAGCATTTGATTTTAGTTTTTTCTTTGTTTCTGTATCAGCTTTGTTTATTCATTTCTGTAAATATGCTTGTTGTTTTAATTTATTAATTTGCTTATTAGTTATATCTATATATGAAGCACAGACACCAGACTCGACACTGACACGTCGACCACGTCGACATGGTAATATCCTGAGAAAGTCAATTATTTGAATGTAGTCACTTGTGTTGCGTCGGTCACCAGACACGCCTTTGATCAGAGGTGTCGGTGCTAGCACAGAGTACATAGTTGATTTAAATATGATTTTACTCGCTTACACGCGTTTTTTCATTTTGGTCATGTAAGTTTTTTTGGTTAGATTCAGTCCTTGTAAATTCAAAACTTGTTGTTTTGAGTCCCTGACGTTAGCTTATTGTGTCACGTGGCGTGATTTTGTTGGGCTAGATATAACGTCTGGGATTAAGAGCAACGATTTTTGAATTTGTAGGGATTGAATCTAAACAAAAATACTTACAAGGACTAAAATAAATAAAAAATATAATTGCAAGGGCCATAATCATATTTAAATTTATGGTTTGATTGGTTGGTATCATGGAACATAAATATGGTTGGTTGGTAGTCAGAACTTCAGAGATAAGGACTTGTTAGCTTCTGGTTGTTTGAATTAATTAGTTAGTTAGATAATTAATTTTATTATTATTGACTATTTGTTTTGTTTTCATCTTGTAATGTAGTCATTTAAAGTTTTCATTTTGTATAGTACTATAACTATTTTGCAAATTTCATCTGAAGTAAAAAGTTGAAAATACAGTTTTACCCCCTCTAATCATATCATGGTTGTATGTTAAAAGTTGATTAGCATGCAAAGAGAGTTGCATTTTTCATTTTGGGGAAAACGTTTGAAATTTTTGAAGGATACAAGATATATAATTTCTGGCAGAGACTACATATAAAGCATTGACACGGACACAAACACCAGACATGACTATAACATGTGGATAATGTGACCAACCATCTGTCTAGGTGTCCAATACCGGACACGTCTTAAATATGAAGTGTTGGTGTTACGTAAGAGACTAGTACATGAGTGGTCTTGCTTTAGGAATGTAAATTTAGTCATTGGATATGCATGCATGTTTTGTGACTTGGTATGTTTTGTTCAATGTCATGTATCTATTGTGATCAACTTATCATTCTAAGTACATGTTTGCTTCCATATGATATATGTTTTGAAGCATCTTTTTTCTTAGGTTTATGGTGACATAATTTACTTAAACCTATGCGTATTCTTAGCCATTGATTGCATTCTCTACTATAATTTATCGTGCAACTTTAAGATTATGATTCCCTGGCTGTTAAAATTGTATATCTATTACTTCTTTCTGAATGGTTGGAGCTATGTATTTCACTTTATTGTAAGGTTGTTTGCAATTTGCATTGCTTTCTTGAATGTTTAGTAGGAATGACCTCCAAATTTTAACTCCAAAGTTGTGCTCATGTTGGAGGAAGCCAGATTTGACTCATGATTTACTGTTTAATGTCAATTGTTGTTGTAATAGATATTTACCCATGCCGCACGTTTTGTAAAATTTCAAGGTAAAGTAATGGCTGTGGCTTTGGATTCTGCTCAAGAAGTTACAACAACGAAAAAACCTCCTATAGAGCAAAGACGAGTAGTTGTGACGGGTATGGGTGTGGTCACACCACTTGGTCATGATCCAGATGTCTTCTACAATAATCTACTTGATGGTGTTAGTGGGATAAGCGAGATTAATGGTTTTGATTGTGCACAATATCCAACGGTAAAGAATATTTCTTCCGAAGTTCATTCATATTCGTTGCCTTTTGTAGTTTTTTGATATTAAGTATTCTCTGTTTCCAGAGGATTGCTGGGGAGATCAAATCTTTCTCAACTGATGGCTTTGTAGCACCAAAGTTTTCAAAGAGGATGGATAAATTTATGTTGTATCTGCTGACAGCTGGAAAAAAAGCCTTGGTCAATGCTGGAATTACTGAAGAAGTAATGGATGAGTTAGATAAAACAAAATGTGGTGTTTTGATTGGCTCCGGAATGGGTGGAATGCAGGTAATCACTCTCTTGAATTTTTAGATTATGCTTTAGCACATTGAGATAAGAGACAGTTGTCCCATTCAGCAGTTTACTTTATATTTAGACGAAGCATAATAACATTCGTTTGTAGGAAGGTGTTATTGTTAGTAATAAGCCACCTGGGGGCAGAAGTTATATATACTAGTAAATTGTTTACTTAATATATCATAAACTAGTAACTGTTACATGTGCAGTAACCAATCTGCAGTAACTGTTATCTGCAGAACTTTTATAACTGCTATAGGGAGTTAGTTTTATAAATAAAGATGCAGAGGAAAAGAAGGGAATTCAGTTATTTGGATAAAGGATTGGTAGGGAGAGACCAATCTGTGTAATTCTTGGAACGGAGAGACCCGAGACTCTCAGATTTCTTGGGAACCCATTGTAATTATACCGACACGGCTGAGATATGTTTACGAAAAGTTATTCATAAATACACATTGTTTGTAAAGAGAAGTTCACATTGCTAATTGCGTATTGCTTATTTATACATAAACATATCAATAGTCACTATATAATGATCACAATTATGTAGTTGTGATCAGGACAAGTAGGCACCACATGTTTGTTTTCATGTTCACAACCTATTCACCAAGTATTAATTTGAAGGTTTTAGTCGTAGGTTTGCAACGGGAGTGCGTTAGTCCAATTGGCCACCGTTCCAAACATAATGCTTACATTACATATCTTTCCCCCATCTATATCAGAATATACGATTTCTGTTACTGTAGCCTAGATCCTGAGCTGGCTCATAGATCGTCGATTCTACTATCCACAACCATGCTTCTGTGCAGTGATACAGCCAGGACTGCTGGAGGTGTGCAACGTGGTCGAACTGGCGTCGTGAGTTTGAGGTGGGATTGTAGATCACTCGTTGGGCCAACCGTTTGGCAACGATCCTTGCTGAAATGGGCAGAGTATTCCTTCTTCCTCTTCCCTACGACAGCGTTTGTATTTGCGCTGCCACCAACAGCGACTCCGTTTTCAAGATCCTACGTAGGATCGGGTGGGGGTGAAAAATCGTATCCGGATTTGTGTCGGAAAAACATACAATTTTGTGTCTGTGATTAGTATGATGTAGCAAAGATTTATCTTATCCGGATGTCAATTATGTTTTTAGATGGTAAGTATCAGTGAAAATATTTTTTCATAGAACTAAAGATTATATTAGATACTTTCTAGGTACAAATCTGAAAGTATTGGACGTGTATTCGTATCAGATACGCGTCGGACACTGATAAATTTCCATTTTTGAAGTATTTGAGTTTCAGAGGTTATCTCTGATGTCGTGTAAAATGAAATTACTGTCAAATGCGATCCAAGATGGTGCAATAAGGCTTTAATATTATGTAAGTTGGTTGCTTTGATTTATTGACTATTTAGTTTCCGTTGTTTGTGCTGAACAGGTTTTTCATGATGCCATTGAAGCTTTACGAATCTCATATAGGAAGATGAATCCTTTTTGTGTACCATTTGCAACAACAAATATGGGTTCTGCCATGCTTGCAATGGATCTGGTCTGTTTAAATAAATAAATAAATTTAAATGCCAAGAACATATTATCGACTCCATAGATGCGTGCCTTGTTTTTTGTCTCAAGTTTCCGACAAAAGATTTTTCTTTTGAAAATTGTAGGGGTGGATGGGCCCAAACTATTCAATATCCACAGCGTGTGCTACAAGTAATTTTTGTATATTGAATGCTGCAAACCATATAATTAAAGGTGAAGCTGTAAGTATTCCACTTCATCATTTGCTTGTTGGATATCTCATTTTTTCCAAACTATTTGTCTCTTAGAAGAAGTTTGGGATAGCTATATGGTTATCTTATTAAACGACATTAAAAATTTGATTTTACCTTTGCAGGACGTGATGCTTTGTGGTGGCTCAGATGCTGCTATAATACCAATTGGTATCCTATTTTCACCAAATCTTTTTGGCTTGCATGAAGTTATCTTGGCATCACTGTAATATTAATCCAACAATCTTGGTGTAGGCTTGGGTGGCTTTGTAGCATGCAGGGCCCTCTCACAAAGAAATAATGATCCTACTAAAGCTTCACGCCCTTGGGATATTGTAATATTATGGATCTTTCTTGTTTTCTTTTCCTATTATTATTGCTAAGTTTTCATTAACCATTTTTGACGTTAATGAAAGTTGAACCCTGCAAAATGTTTTAAAAATTTCATGATATCCATAATTTGAAGTGGAAAAATCCTTGAGAAATGAAAAAGAGAAACTTTGGAGGCATCCAACTGAATAAACATTAGTGAAAAGCTTTACTCTATTCCTAAAATGAGCAAATTTGTCTGCACCTGCTTCAAAATCTAAATTTAAATATATAAACAACTTAATTCTTGATGAAGTGATGGATGCATTAAGTGCAAAGTACAAAGGATACTAAACAATTATAGTGCTTGCAGTTTTATTCCATAGTCTTTTGTCTTATTATTCTGCACTGCTGTTGTTAACGATTAGCATACCTGTGTGATTTTACAGAACCGTGATGGATTTGTAATGGGTGAAGGGGCTGGAGTTTTGCTTTTAGAAGAGTTAGAGCATGCTAAGGTACTCTTATTTTATTCTATCAATTTAAAAATAAACGCAACTTAACTTTACAGCCTGATGTTGAATGTTAATGTGATGCTTATTTACCCAATTAATTTTCTATCCTGTCCTTCATTTTCTTTCAAAGGCATAACACCACAGTTATGTATCCATTGTCGTATTTCTTGTTAAAATCATGCTTCATACTTATTGTCATAAGTACTTTATCATTTTCATCACATTACATTGTTTTGGCAAATCATATTCAATTAAGTGAACTAACCGATATTGACATCTAGCTTTTTATATTTCAGAATAGGGGAGCAACCATATATGCAGAGTTCCTCGGTGGAAGCTTCACATGTGATGCTTATCATGTGACTGAACCACGTCCTGATGGTAAAATATTACATCTTCTTCAAGACCAATTCCTTTCGGATGATCTTAAAAACATAAATATTTGGTGGGTTTTGTGACAACTTACGGAGATTGTAGACTAAGGCTCTGTTTGATAAGAAGTAGTGGATGACAGAAACATTAGTTAGTAGCGAAGAAGCTTACAGTCGACAACCTAGTTGATAGTGGATGAACTAACAAATTGAATTTGATGAGTTTGATATAAGTAGTGGTTGAACCAAATGATAGATATAAAATGGCATTAAAAGATATTTTTAATTAAAATTTAATTTTTTTAATTAAGATAATGACGGTAAAAGTGAAAAAACACTATAAGCTCGTGGGAGAAGTACCGTTACTAAACAATTCTATTTTGATCATACGAGCTTATAAGCTATAAGCTAGTGATTTGTCCTTGCCAAACTGAGTCTAATATGTTGATTCAATAATCTTATCTTGGATCTTATAGTTCCTTCCCCCAAGTTTAAGGCCAGGAGAGGAAGGACCAATGAAGTCATGTCTTAGCCTGCGAAAGAATGACACCAACCTCCATCAATTGAAACAATGTCAACGAATTTGTAGATGTTATATTTACCTTAACAACAGAAATCCATTTAAAAAAGGTCAAGAAAACAAGAAAAAATGCATTTGGGGTAGAAAACAAATTAAAGAGAAAGGAGATATTTACTTCTGAACACTTTATTATGATTGTGGAGGAGTAATAATGAGCTAAATCTTCAATATTTTTGTTCAACCTTGGTTAAGGTAATTTGTGTTGCCTACGATAAAATAATACATTGTTGTTCTTATTGACTGCTTTCACTCCATTTTTACCCATAAAGCATAACGTTTTTTAATTTATGCGAAAGCTCTCACCCATGACATGAACTTTCTTACATGTTAATGTCTAAAATGTAGGGCAGCAGAACACTGCATACACATGCATGTTTTGCATATAATATAGGTAGCATATTAGTTGAAAACATAAAAGGTATCGATACCCTTGACTTTTCATATATTGTTATATGATCAAGATTAGCCTCTCCCACTTCTCGTGCTTCACTTGATACGTTCCCTTTATGTGTTGGGTACAAGGATGAAAAGAGGAAGGAGCTGTATTTTTAAATCAAGTGTATTAGACATCTATCTTAGCATTTCTCAAGTTTCAACCTTTTTTTAATATACTTTTGTTTATTTTTTCTAATTTTTTTTTTAAACATTGTGAATTTTATCGTAAGACTAGATGGGATAGGATTGGGAATGAAAATATTAGAGAGGGAGAGAGAGTTGGGGTAGCACCTATAGTAGAAAAGATGGTGGAAAATAGGCTTTAAGTGGTTTGGGGATGTAGAGAGTAGACCTGCAGATTCTATAGTAAGGAGAGTAGATCAGATGGAGGATAGTCAAATCACTATAGGCAGAGGAAGACCTAGAAAAACTATAAGAAATATTAGGAAAAACTTAGAGATTAATGAGTTGGATCAAAATATGGTTTATGATAGAACATTATGGTGTAATTTGATCCATGTAGCTGACCCCACTTAGTGGGACAAGGCTTGGTTGTTGTTGTTGAGTCACGCTTTTCAAGTGTCAAGCCTTATTTAAATTGTTGGCTTTGTGTCCTAGTGCTTGGCGTGCGTCTAAGCTGATGAAAATATACTTCTTTAACAAGACATCTGATGAGGAGTTTTCAACACACAACATATGAGTGTCATATGGTTTCGGTGTTGGAAGTGTTAAACACAACATCTTAAGAAGGAGCTGTGGCTGTGCTTCATAAGAATGGGGAAAAAAAAAAGGCCTGTTTGGAAGATTGTTTTCACCATAAAGACTGATGTTAGAAATTCTGTCTTATTTTCTTTCCGCCCCTAGCGGCCTTAATGGCGATATTTGGGTTCCCTTCATTGCAGCCACCAGCGCCTTCATTGTGCTGGGTGTGAGGGGGTGAGTTTAGTCTCACGCCACTTAGAGATATGACTTTAGTGTTTATAAACTTGGGTAGTCCTTACTTTACCATCCAGTTTTCTATGGTTGAGTAATACTAAGAACTGACCATGATTTTCTGTTTTGTTGTTGTCGAAGAACAGATATCATACCACTTTTTTTTGGTTGACCAAAGAACTAAATATGTTTTCATAATTTTATTAATGGCAAAACTGTAATAATATAAGTATTTCTTTGCATATGTTTTTGTGTTCAATAGTGATTATTTATAATAAAAAATTTACCAAGACATATTTCCCTGTTTTATGGCATCGTGTCATCTATCTAATTTGCACCATTTAGTATGGAATGGCTACTGCTGTTGCTATAGTAATTGTAATTGGTCTCAAATCCCTTCTTGCAAATTGTAAAAACTGTTTTCATGGTTATTTTGCTTGTTTTTTCTTCGTCTATGATAATTACAGGGCGCAATGATCATCATGTATTAGGCTGAGGCATGTTTTGTTAGAAAATAATGTTGTCCTATTACTTTTTTGAAATGGATGCTAAAGCTTTTTGGTCAAAAGCATATTTTGTCACTTATAGTGATAGTTGTTATTTGTATCTTTACAAATTCTCAACATGATTGTAAATTTTTGCACACTAATCATCTTATCTTCTAACTATCGAAATGCAATTTCACATTGTCACTTTGACCATGATAATTTAGAGATTATGCCAACTACATCTTCCAAATGATTCCAAAGTAAAACATTTTCCCATCACTTTCTTATAGGTTTTTAAATACACTTTGTTGACTGTACTGTACTGGGTGAGTATGTGAATCATGAATATCTGTAGATCTCTTAAAGAAAATTTGGTTCTGCATATCCCTGCAAATCATGTCTTGATTATGCTTGCACTTATGTTTCTCTACTCAACAACTACAGGGGCTGGTGTTATTCTTTGCATTGAAAAGGCATTAGCTAATTCTGGAATTTCAAAGGAGGATGTGAACTACATAAATGCACATGCCACATCCACACCTGCTGGAGATCTTAAGGAGTACCAGGCTTTGATTCATTGCTTTGGCCAAAATCCTGAGGTAAACTTTCTTTCATGTGTATTTATTACTATATTCTCCCTAATCACTGTAAAGGTAATTATAATTGTTTGCCCTTTTATTTCTTTCGATTTATGGTACAGCTAAAAGTGAATTCGACGAAGTCTATGATTGGTCATCTACTAGGAGCAGCTGGTGGTGTGGAAGCCGTTGCAACTATACAGGTGACAACTATTGTGCCACTTGTTTATGAATTTGTTCCAGTAACCTAAATGTTCTGACAAAAAAAATGAAGATTTTTGTTCTTTTTGTTTGACTTCATGTAGGCAATAAAGACAGGGTGGGTTCATCCTAATATCAATCTAGAAAACCCGGATGAAGCAGTGGTATGATACTTGTTGCACCATTCTCTCCTATGTTTGAAAATGTGTTGTGTCATACCACCTCCATCCCTTATTATAAGCTTTTATGTTTTCACATATATCAAGAAAAGTAGGGAGTGTATTTAATGTGTAAATTTTGTTTATAGATGTTCCTAAACTACCCTTTTATGTATAGTTGTGTCTAGCTTTGACTTTTCATCAGCCAAGATAAACTTATACTCCCTCCGTCCCTAATTGTAAGACCATTTATAAAAAATGCTTTGTCCCTAAATATAAGACCATCTACCAATTTCTAGATGCATTTATTACATTTTTTTTTTCTTTCAAATATACTCCTTATTGATCCTATCAGGGCCGTATCTGAGGCAGGGCGAAGAGGGCGACCGCCCTAGGCCCCAAAAAATAAGTAAGAAATTCGGGGCCTAAATTTTTATTTTAGGGTTCCATTAGGTCCTAACAAAATTGCTTAGTTATTTTTCTGCTCCAACAACATTATTAAGGGTTCCATTAGGTCCTAACATCCATGACAAATTTAAGGTTCCATTAGGGTTCCATTAGGTCCTAACATCCATGACAAATTTATTACTCCAACAACCTTTCTTAATACCCGTGATTGTGTAAGACGGTCTTATAAAAAGGGATGGCGGGAGTAGTATTAATAAGGGGTTTATTTAAAAAAGTAATAATAAATTTATCCAGTAATTTGAAACTGAACTTATATTTAGGTTTTCTTTTTCCTTTCAAAAATGGCTTATCGTGAGGGACAGAGGTAGTATCATTTATGGGGTCATTATAGTTATCTAATTTTTTTTAATTGAAAACAAAAACCAGGACACCAATGTGCTTGTGGGATCAAAGAAAGAGAGACTGGACATTAAGGCAGGCTTGTCTAATTCATTTGGTTTTGGTGGCCACAATTCTTCAATCATTTTTGCCCCTTTTAAGTGA
Protein sequence

>MS.gene025314.t1

MLVADRLVDQPSAVTCDSHSSQSHRSRRRSRNNHLNLSSPNRMSRSGKVMAVALDSAQEVTTTKKPPIEQRRVVVTGMGVVTPLGHDPDVFYNNLLDGVSGISEINGFDCAQYPTRIAGEIKSFSTDGFVAPKFSKRMDKFMLYLLTAGKKALVNAGITEEVMDELDKTKCGVLIGSGMGGMQVFHDAIEALRISYRKMNPFCVPFATTNMGSAMLAMDLGWMGPNYSISTACATSNFCILNAANHIIKGEADVMLCGGSDAAIIPIGLGGFVACRALSQRNNDPTKASRPWDINRDGFVMGEGAGVLLLEELEHAKNRGATIYAEFLGGSFTCDAYHVTEPRPDGAGVILCIEKALANSGISKEDVNYINAHATSTPAGDLKEYQALIHCFGQNPELKVNSTKSMIGHLLGAAGGVEAVATIQAIKTGWVHPNINLENPDEAVDTNVLVGSKKERLDIKAGLSNSFGFGGHNSSIIFAPFK