Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026533.t1 | XP_024638642.1 | 93.8 | 128 | 8 | 0 | 1 | 128 | 1 | 128 | 2.20E-57 | 232.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026533.t1 | Q9CX48 | 55.9 | 34 | 15 | 0 | 86 | 119 | 20 | 53 | 5.8e-06 | 52.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026533.t1 | A0A396IFI3 | 93.8 | 128 | 8 | 0 | 1 | 128 | 1 | 128 | 1.6e-57 | 232.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene066824 | MS.gene026533 | PPI |
| MS.gene045256 | MS.gene026533 | PPI |
| MS.gene75592 | MS.gene026533 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026533.t1 | MTR_1g032030 | 91.045 | 67 | 5 | 1 | 18 | 83 | 15 | 81 | 2.86e-22 | 89.4 |
| MS.gene026533.t1 | MTR_1g032370 | 91.045 | 67 | 5 | 1 | 18 | 83 | 15 | 81 | 2.86e-22 | 89.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026533.t1 | AT5G49400 | 74.803 | 127 | 24 | 2 | 9 | 129 | 16 | 140 | 4.96e-48 | 159 |
Find 52 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTAGTCTGCTTCTTCTTC+TGG | 0.203184 | 1.1:+17328662 | None:intergenic |
| GACACATCCGGCCTCAATTT+AGG | 0.222584 | 1.1:+17328907 | None:intergenic |
| ATCAGATTATTCTTCTAGTT+CGG | 0.272097 | 1.1:-17328740 | MS.gene026533:CDS |
| TAGTAAGAAGGAAGAGATTA+AGG | 0.276310 | 1.1:-17329259 | MS.gene026533:CDS |
| ACACATCCGGCCTCAATTTA+GGG | 0.281606 | 1.1:+17328908 | None:intergenic |
| GGCCGCAGTTGCTGCTCTTT+CGG | 0.294122 | 1.1:+17329194 | None:intergenic |
| TCAGATTATTCTTCTAGTTC+GGG | 0.343157 | 1.1:-17328739 | MS.gene026533:CDS |
| CCATCGATAGTCGACATTGT+AGG | 0.393513 | 1.1:+17329036 | None:intergenic |
| GTCAAAGAGGAAGCATGATT+CGG | 0.395633 | 1.1:-17328800 | MS.gene026533:CDS |
| TCAAAGAGGAAGCATGATTC+GGG | 0.417245 | 1.1:-17328799 | MS.gene026533:CDS |
| CAGCATTAATTGCTGCGAAA+GGG | 0.433572 | 1.1:-17329233 | MS.gene026533:CDS |
| TGTGTCTGTGGATTATGATT+TGG | 0.452148 | 1.1:-17328890 | MS.gene026533:CDS |
| GCGCGTAATGTTAACGCATA+TGG | 0.458903 | 1.1:-17329171 | MS.gene026533:CDS |
| CAGAGGAAGAAAGGAGAAGG+AGG | 0.477099 | 1.1:-17328696 | MS.gene026533:CDS |
| ATGAGCGGGTTTATATTTCA+AGG | 0.478841 | 1.1:-17328957 | MS.gene026533:CDS |
| GCAGCATTAATTGCTGCGAA+AGG | 0.482444 | 1.1:-17329234 | MS.gene026533:CDS |
| CATGATTCGGGTGTTGATAG+TGG | 0.485011 | 1.1:-17328787 | MS.gene026533:CDS |
| CGTCTGAATCAGAGGAAGAA+AGG | 0.505020 | 1.1:-17328705 | MS.gene026533:CDS |
| AGTGTTACCAGGGTGGGCAC+TGG | 0.514214 | 1.1:-17328996 | MS.gene026533:CDS |
| GAATATGTTGAGTAGTAAGA+AGG | 0.518238 | 1.1:-17329271 | None:intergenic |
| CAGTGGATCATCGTCTGCCA+CGG | 0.518690 | 1.1:-17328764 | MS.gene026533:CDS |
| GCAGTGTCAGAAGTGTTACC+AGG | 0.520961 | 1.1:-17329007 | MS.gene026533:CDS |
| TGCTACAGTGGTTGAAAGGA+AGG | 0.521953 | 1.1:-17329067 | MS.gene026533:CDS |
| GCATATGGTCAGAAGGAAGA+AGG | 0.525781 | 1.1:-17329156 | MS.gene026533:CDS |
| TACTGAGAAAGCTGCTACAG+TGG | 0.535042 | 1.1:-17329079 | MS.gene026533:CDS |
| TCATAATCCACAGACACATC+CGG | 0.535556 | 1.1:+17328895 | None:intergenic |
| AGTGGATCATCGTCTGCCAC+GGG | 0.553302 | 1.1:-17328763 | MS.gene026533:CDS |
| AGAAGGGCCTAGCAGATGGC+AGG | 0.555494 | 1.1:-17329139 | MS.gene026533:CDS |
| TTAAGTTGCTGAGTCCTCGA+TGG | 0.557112 | 1.1:+17328934 | None:intergenic |
| CAGCTCATCGTCTGAATCAG+AGG | 0.559637 | 1.1:-17328713 | MS.gene026533:CDS |
| TCGGGTGTTGATAGTGGCAG+TGG | 0.566877 | 1.1:-17328781 | MS.gene026533:CDS |
| ATTTCAATGTAAGAATGAGC+GGG | 0.569709 | 1.1:-17328971 | MS.gene026533:CDS |
| AGGAAGAAGGGCCTAGCAGA+TGG | 0.572751 | 1.1:-17329143 | MS.gene026533:CDS |
| AAGCTGCTACAGTGGTTGAA+AGG | 0.575356 | 1.1:-17329071 | MS.gene026533:CDS |
| AGCCGAAAGAGCAGCAACTG+CGG | 0.577539 | 1.1:-17329196 | MS.gene026533:CDS |
| TCGTGATGTGAAGGAGGAGA+AGG | 0.580022 | 1.1:-17328842 | MS.gene026533:CDS |
| TGTTAACGCATATGGTCAGA+AGG | 0.582981 | 1.1:-17329163 | MS.gene026533:CDS |
| TAATAATCATCGTGATGTGA+AGG | 0.585747 | 1.1:-17328851 | MS.gene026533:CDS |
| CATTTCAATGTAAGAATGAG+CGG | 0.604438 | 1.1:-17328972 | MS.gene026533:CDS |
| GTCAGAAGTGTTACCAGGGT+GGG | 0.604446 | 1.1:-17329002 | MS.gene026533:CDS |
| CAGACTAAGAAGAAGAGCAA+AGG | 0.608854 | 1.1:-17328649 | MS.gene026533:CDS |
| AATCAGAGGAAGAAAGGAGA+AGG | 0.616394 | 1.1:-17328699 | MS.gene026533:CDS |
| CATATGGTCAGAAGGAAGAA+GGG | 0.616780 | 1.1:-17329155 | MS.gene026533:CDS |
| TAATCATCGTGATGTGAAGG+AGG | 0.624901 | 1.1:-17328848 | MS.gene026533:CDS |
| CCTACAATGTCGACTATCGA+TGG | 0.625467 | 1.1:-17329036 | MS.gene026533:CDS |
| ATTGAGGCCGGATGTGTCTG+TGG | 0.648025 | 1.1:-17328902 | MS.gene026533:CDS |
| GAAATGTCCAGTGCCCACCC+TGG | 0.650305 | 1.1:+17328989 | None:intergenic |
| TGTCAGAAGTGTTACCAGGG+TGG | 0.665235 | 1.1:-17329003 | MS.gene026533:CDS |
| CAGTGTCAGAAGTGTTACCA+GGG | 0.669839 | 1.1:-17329006 | MS.gene026533:CDS |
| AGAAGAATAATCTGATCCCG+TGG | 0.687932 | 1.1:+17328747 | None:intergenic |
| TTTATATTTCAAGGCCATCG+AGG | 0.711495 | 1.1:-17328948 | MS.gene026533:CDS |
| ATATGCGTTAACATTACGCG+CGG | 0.742150 | 1.1:+17329173 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACTTAAAAACCCTAAATTG+AGG | - | chr1.1:17328973-17328992 | MS.gene026533:CDS | 25.0% |
| !! | ATCAGATTATTCTTCTAGTT+CGG | - | chr1.1:17329151-17329170 | MS.gene026533:CDS | 25.0% |
| ATTTCAATGTAAGAATGAGC+GGG | - | chr1.1:17328920-17328939 | MS.gene026533:CDS | 30.0% | |
| CATTTCAATGTAAGAATGAG+CGG | - | chr1.1:17328919-17328938 | MS.gene026533:CDS | 30.0% | |
| TAATAATCATCGTGATGTGA+AGG | - | chr1.1:17329040-17329059 | MS.gene026533:CDS | 30.0% | |
| TAGTAAGAAGGAAGAGATTA+AGG | - | chr1.1:17328632-17328651 | MS.gene026533:CDS | 30.0% | |
| !! | TCAGATTATTCTTCTAGTTC+GGG | - | chr1.1:17329152-17329171 | MS.gene026533:CDS | 30.0% |
| TAAAAACCCTAAATTGAGGC+CGG | - | chr1.1:17328977-17328996 | MS.gene026533:CDS | 35.0% | |
| TGAGTTCGAAAAAGTCAAAG+AGG | - | chr1.1:17329078-17329097 | MS.gene026533:CDS | 35.0% | |
| TTTATATTTCAAGGCCATCG+AGG | - | chr1.1:17328943-17328962 | MS.gene026533:CDS | 35.0% | |
| ! | ATGAGCGGGTTTATATTTCA+AGG | - | chr1.1:17328934-17328953 | MS.gene026533:CDS | 35.0% |
| ! | TGTGTCTGTGGATTATGATT+TGG | - | chr1.1:17329001-17329020 | MS.gene026533:CDS | 35.0% |
| AATCAGAGGAAGAAAGGAGA+AGG | - | chr1.1:17329192-17329211 | MS.gene026533:CDS | 40.0% | |
| AGAAGAATAATCTGATCCCG+TGG | + | chr1.1:17329147-17329166 | None:intergenic | 40.0% | |
| AGAAGAGCAAAGGAAAAAGC+AGG | - | chr1.1:17329252-17329271 | MS.gene026533:CDS | 40.0% | |
| ATATGCGTTAACATTACGCG+CGG | + | chr1.1:17328721-17328740 | None:intergenic | 40.0% | |
| CAGACTAAGAAGAAGAGCAA+AGG | - | chr1.1:17329242-17329261 | MS.gene026533:CDS | 40.0% | |
| CAGCATTAATTGCTGCGAAA+GGG | - | chr1.1:17328658-17328677 | MS.gene026533:CDS | 40.0% | |
| CATATGGTCAGAAGGAAGAA+GGG | - | chr1.1:17328736-17328755 | MS.gene026533:CDS | 40.0% | |
| TAATCATCGTGATGTGAAGG+AGG | - | chr1.1:17329043-17329062 | MS.gene026533:CDS | 40.0% | |
| TCATAATCCACAGACACATC+CGG | + | chr1.1:17328999-17329018 | None:intergenic | 40.0% | |
| TCTTAGTCTGCTTCTTCTTC+TGG | + | chr1.1:17329232-17329251 | None:intergenic | 40.0% | |
| TGTTAACGCATATGGTCAGA+AGG | - | chr1.1:17328728-17328747 | MS.gene026533:CDS | 40.0% | |
| ! | GTCAAAGAGGAAGCATGATT+CGG | - | chr1.1:17329091-17329110 | MS.gene026533:CDS | 40.0% |
| ! | TCAAAGAGGAAGCATGATTC+GGG | - | chr1.1:17329092-17329111 | MS.gene026533:CDS | 40.0% |
| AAGCTGCTACAGTGGTTGAA+AGG | - | chr1.1:17328820-17328839 | MS.gene026533:CDS | 45.0% | |
| ACACATCCGGCCTCAATTTA+GGG | + | chr1.1:17328986-17329005 | None:intergenic | 45.0% | |
| AGAGCAAAGGAAAAAGCAGG+AGG | - | chr1.1:17329255-17329274 | MS.gene026533:CDS | 45.0% | |
| CCATCGATAGTCGACATTGT+AGG | + | chr1.1:17328858-17328877 | None:intergenic | 45.0% | |
| CGTCTGAATCAGAGGAAGAA+AGG | - | chr1.1:17329186-17329205 | MS.gene026533:CDS | 45.0% | |
| GCAGCATTAATTGCTGCGAA+AGG | - | chr1.1:17328657-17328676 | MS.gene026533:CDS | 45.0% | |
| GCATATGGTCAGAAGGAAGA+AGG | - | chr1.1:17328735-17328754 | MS.gene026533:CDS | 45.0% | |
| GCGCGTAATGTTAACGCATA+TGG | - | chr1.1:17328720-17328739 | MS.gene026533:CDS | 45.0% | |
| TACTGAGAAAGCTGCTACAG+TGG | - | chr1.1:17328812-17328831 | MS.gene026533:CDS | 45.0% | |
| TAGCAGATGGCAGGAAAAAC+AGG | - | chr1.1:17328761-17328780 | MS.gene026533:CDS | 45.0% | |
| TGCTACAGTGGTTGAAAGGA+AGG | - | chr1.1:17328824-17328843 | MS.gene026533:CDS | 45.0% | |
| ! | CCTACAATGTCGACTATCGA+TGG | - | chr1.1:17328855-17328874 | MS.gene026533:CDS | 45.0% |
| ! | TGTTTTTCCTGCCATCTGCT+AGG | + | chr1.1:17328762-17328781 | None:intergenic | 45.0% |
| ! | TTAAGTTGCTGAGTCCTCGA+TGG | + | chr1.1:17328960-17328979 | None:intergenic | 45.0% |
| !! | CAGTGTCAGAAGTGTTACCA+GGG | - | chr1.1:17328885-17328904 | MS.gene026533:CDS | 45.0% |
| !! | CATGATTCGGGTGTTGATAG+TGG | - | chr1.1:17329104-17329123 | MS.gene026533:CDS | 45.0% |
| AGGAAAAACAGGAGGCGAAG+AGG | - | chr1.1:17328772-17328791 | MS.gene026533:CDS | 50.0% | |
| CAGAGGAAGAAAGGAGAAGG+AGG | - | chr1.1:17329195-17329214 | MS.gene026533:CDS | 50.0% | |
| CAGATGGCAGGAAAAACAGG+AGG | - | chr1.1:17328764-17328783 | MS.gene026533:CDS | 50.0% | |
| CAGCTCATCGTCTGAATCAG+AGG | - | chr1.1:17329178-17329197 | MS.gene026533:CDS | 50.0% | |
| GACACATCCGGCCTCAATTT+AGG | + | chr1.1:17328987-17329006 | None:intergenic | 50.0% | |
| TCGTGATGTGAAGGAGGAGA+AGG | - | chr1.1:17329049-17329068 | MS.gene026533:CDS | 50.0% | |
| !! | GCAGTGTCAGAAGTGTTACC+AGG | - | chr1.1:17328884-17328903 | MS.gene026533:CDS | 50.0% |
| !! | GTCAGAAGTGTTACCAGGGT+GGG | - | chr1.1:17328889-17328908 | MS.gene026533:CDS | 50.0% |
| !! | TGTCAGAAGTGTTACCAGGG+TGG | - | chr1.1:17328888-17328907 | MS.gene026533:CDS | 50.0% |
| AGCCGAAAGAGCAGCAACTG+CGG | - | chr1.1:17328695-17328714 | MS.gene026533:CDS | 55.0% | |
| AGGAAGAAGGGCCTAGCAGA+TGG | - | chr1.1:17328748-17328767 | MS.gene026533:CDS | 55.0% | |
| AGTGGATCATCGTCTGCCAC+GGG | - | chr1.1:17329128-17329147 | MS.gene026533:CDS | 55.0% | |
| ATTGAGGCCGGATGTGTCTG+TGG | - | chr1.1:17328989-17329008 | MS.gene026533:CDS | 55.0% | |
| CAGTGGATCATCGTCTGCCA+CGG | - | chr1.1:17329127-17329146 | MS.gene026533:CDS | 55.0% | |
| !! | TCGGGTGTTGATAGTGGCAG+TGG | - | chr1.1:17329110-17329129 | MS.gene026533:CDS | 55.0% |
| AGAAGGGCCTAGCAGATGGC+AGG | - | chr1.1:17328752-17328771 | MS.gene026533:CDS | 60.0% | |
| AGTGTTACCAGGGTGGGCAC+TGG | - | chr1.1:17328895-17328914 | MS.gene026533:CDS | 60.0% | |
| GAAATGTCCAGTGCCCACCC+TGG | + | chr1.1:17328905-17328924 | None:intergenic | 60.0% | |
| GGCCGCAGTTGCTGCTCTTT+CGG | + | chr1.1:17328700-17328719 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 17328624 | 17329289 | 17328624 | ID=MS.gene026533 |
| chr1.1 | mRNA | 17328624 | 17329289 | 17328624 | ID=MS.gene026533.t1;Parent=MS.gene026533 |
| chr1.1 | exon | 17328624 | 17329289 | 17328624 | ID=MS.gene026533.t1.exon1;Parent=MS.gene026533.t1 |
| chr1.1 | CDS | 17328624 | 17329289 | 17328624 | ID=cds.MS.gene026533.t1;Parent=MS.gene026533.t1 |
| Gene Sequence |
| Protein sequence |
